Scribd Logo
Upload

Welcome to Scribd!

  • 8K views

    Salkowski Test & Conclusion

    Uploaded by

    JezKagandahan
    The document summarizes the Salkowski reaction, which is the reaction of cholesterol with concentrated sulfuric acid. In this reaction, sulfuric acid removes two water molecules from two cholesterol molecules, connecting them at position 3 to form bicholestadien. The acid also sulfonates the bicholestadien molecule at the 7,7' positions of the aromatic ring, ultimately forming a red bissulfonic acid of bicholestadiene. The Salkowski reaction involves adding sulfuric acid to a chloroform solution of cholesterol, which causes dehydration and formation of a bisteroid that appears red in the interphase between layers.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd

    Salkowski Test & Conclusion

    Uploaded by

    JezKagandahan
    8K views3 pages
    The document summarizes the Salkowski reaction, which is the reaction of cholesterol with concentrated sulfuric acid. In this reaction, sulfuric acid removes two water molecules from two cholesterol molecules, connecting them at position 3 to form bicholestadien. The acid also sulfonates the bicholestadien molecule at the 7,7' positions of the aromatic ring, ultimately forming a red bissulfonic acid of bicholestadiene. The Salkowski reaction involves adding sulfuric acid to a chloroform solution of cholesterol, which causes dehydration and formation of a bisteroid that appears red in the interphase between layers.

    Original Description:

    Salkowski Test & Conclusion

    Copyright

    © © All Rights Reserved

    Available Formats

    DOCX, PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    The document summarizes the Salkowski reaction, which is the reaction of cholesterol with concentrated sulfuric acid. In this reaction, sulfuric acid removes two water molecules from two cholesterol molecules, connecting them at position 3 to form bicholestadien. The acid also sulfonates the bicholestadien molecule at the 7,7' positions of the aromatic ring, ultimately forming a red bissulfonic acid of bicholestadiene. The Salkowski reaction involves adding sulfuric acid to a chloroform solution of cholesterol, which causes dehydration and formation of a bisteroid that appears red in the interphase between layers.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    8K views3 pages

    Salkowski Test & Conclusion

    Uploaded by

    JezKagandahan
    The document summarizes the Salkowski reaction, which is the reaction of cholesterol with concentrated sulfuric acid. In this reaction, sulfuric acid removes two water molecules from two cholesterol molecules, connecting them at position 3 to form bicholestadien. The acid also sulfonates the bicholestadien molecule at the 7,7' positions of the aromatic ring, ultimately forming a red bissulfonic acid of bicholestadiene. The Salkowski reaction involves adding sulfuric acid to a chloroform solution of cholesterol, which causes dehydration and formation of a bisteroid that appears red in the interphase between layers.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    You are on page 1of 3

    Salkowski reaction is the reaction of cholesterol with concentrated sulphuric acid.

    Concentrated
    sulphuric acid is highly hygroscopic and it removes two molecules of water from two molecules of
    cholesterol, it causes a connection at position 3, forming bi- cholestadien . Simultaneously the sulphuric
    acid sulphonates the molecule of bi-cholestadien at positions 7,7’ of aromatic ring and, as a final
    product, red colour bi-sulphonic acid of bi-cholestadiene is formed

    This taste is named after Leopold Salkowski, a German physiological chemist. In this test, only sulfuric
    acid was added in a chloroform solution of the sample. In the presence of the acid, dehydration occurs
    forming a bisteroid which gives a red color interphase (Espino-Cabatit, 1988). The red interphase can
    also be blue. This interphase is observed in the chloroform layer while in the acid layer a green
    fluorescence. This test is done in the sample and the color of interphase was noted.

    Sample Documetation Observation


    Cholesterol Dark red at the junction

    Raizaalmirah. (n.d.). Salkowski reaction is the reaction of cholesterol with concentrated sulphuric:
    Course Hero. Retrieved from https://www.coursehero.com/file/p359l0k/Salkowski-reaction-is-the-
    reaction-of-cholesterol-with-concentrated-sulphuric/.

    bianca5195. (n.d.). The second test performed is the Salkowski test This test is similar to: Course Hero.
    Retrieved from https://www.coursehero.com/file/p77u2la/The-second-test-performed-is-the-
    Salkowski-test-This-test-is-similar-to/.

    Procedure:

    1. A small pinch of cholesterol was placed in a test tube.


    2. Chloroform was added then added drop-by-drop until it is dissolved.
    3. 5 drops of Sulphuric acid was then slowly added at the side of the inclined test tube.
    4. The students stood the test tube slowly without shaking and observed the color at the juncture
    of the two liquids.

    Small pinch The change


    Chloroform 5 drops of
    of of color at
    was added Sulphuric
    cholesterol the
    added acid was
    was placed junction
    drop-by- then slowly
    in a tes was
    drop added
    tube observed
    Conclusion:

    Based on the experiment, the students concluded that all the test results of the samples of cholesterol
    and bile complements the results of the control hence, ensuring that the sample do all contain the
    properties that were in specific tests. In addition, the students determined the chemical reactions
    undergone by cholesterol such as the Leibermann-Burchard Test and Salkowski Test which yielded
    positive results. The properties of bile was also analyzed such as pH value, emulsification value and
    presence of bile salt through Petten Kofer’s Test, which all yielded positive results.

    You might also like