Sonia Malik - Essential Oil Research - Trends in Biosynthesis, Analytics, Industrial Applications and Biotechnological Production-Springer International Publishing (2019)
Sonia Malik - Essential Oil Research - Trends in Biosynthesis, Analytics, Industrial Applications and Biotechnological Production-Springer International Publishing (2019)
Sonia Malik - Essential Oil Research - Trends in Biosynthesis, Analytics, Industrial Applications and Biotechnological Production-Springer International Publishing (2019)
Essential Oil
Research
Trends in Biosynthesis, Analytics,
Industrial Applications and
Biotechnological Production
Essential Oil Research
Sonia Malik
Editor
This Springer imprint is published by the registered company Springer Nature Switzerland AG
The registered company address is: Gewerbestrasse 11, 6330 Cham, Switzerland
Dedicated To my grandparents
Preface
Essential oils obtained from plants are gaining tremendous attention due to their sev-
eral biological properties and use in food, cosmetics, and pharmaceutical industries.
Essential oils are extracted from various plant parts by using different techniques.
The chemical composition and quality of essential oils vary depending on several
genetic and environmental factors. By employing diverse biotechnological meth-
ods, it is possible to improve the production of essential oils from plants. All these
issues have been addressed in this book. Fourteen chapters are written by globally
renowned researchers working in the area of essential oils and natural products.
Chapter 1 by Hanif et al. provides the general overview of essential oils, their
chemistry, extraction methods, analyses, biological activities, applications, risks,
and dangers. The chemical composition of essential oils is influenced by biotic,
abiotic, and genetic factors, which are discussed in Chap. 2 by Boaro et al. Chapter
3 by Sakhanokho and Rajasekaran describes the composition and uses of essential
oils from different species of Hedychium, while the essential oils of family
Burseraceae are presented by DeCarlo et al. in Chap. 4. Chapter 5 by Guha and
Nandi highlights the potential of essential oil of betel leaf in the world food sector,
and Chap. 6 by Desrosiers et al. is focused on essential oils from two different spe-
cies of Artemisia. Activity of essential oils against human oral pathogens has been
detailed in Chap. 7 by Marinković et al. Chapter 8 by Blank et al. is devoted on
chemical diversity and biological activities of essential oils of plants from Northeast
Brazil. Satyal and Setzer discuss about adulteration in essential oils and its analysis
in Chap. 9. Applications of essential oils from pines are presented in Chapter 10 by
Kumar et al. Segura et al. in Chap. 11 address various biotechnological approaches
to improve the yield and quality of essential oil in aromatic plants. The phytochemi-
cal composition, pharmacological activities, and biotechnological production of
essential oils from geranium are summarized in Chap. 12 by Narnoliya et al. Chap.
13 by Banerjee and Roychoudhury presents the potential applications of metabolic
engineering for the enhanced production of aromatic oils in plants. The role of bio-
technology in obtaining essential oils from non-herbaceous plants is documented in
Chap. 14 by Gounaris. Lastly, Chap. 15 by Semenova et al. aims to describe
Eremothecium strains as essential oil producers.
vii
viii Preface
Through this multi-authored book, efforts have been made to provide recent
developments and techniques for extracting essential oils and various applications
of biotechnological methods for their improved production in plants. This book will
be a valuable reference for biotechnologists, pharmacists, food technologists, and
researchers working in the area of natural plant products and medical and healthcare
industries.
Since the beginning of my task in selecting the title of this book and bringing it into
the present form, I have always experienced a special source of inspiration, guid-
ance, and shower of blessings from my grandparents and parents. They have left no
stone unturned in shaping my academic career in an exceptional way. I would also
like to acknowledge my brothers and their families for their love and affection.
A heartfelt appreciation goes to my husband, Dr. Surender Kumar Sharma, for
his valuable advice. I would like to extend my special regards to my mother-in-law
and father-in-law for their kind gesture and encouragement.
I owe my thanks to Springer team for their technical support and time-to-time
suggestions. I also acknowledge all the experienced and renowned authors for their
contributions.
ix
Contents
xi
xii Contents
Index������������������������������������������������������������������������������������������������������������������ 437
Contributors
xiii
xiv Contributors
1.1 Introduction
The attraction of aromatic and medicinal plants grows continuously due to the
increasing demand as well as interest of consumers in these plants for medicinal,
culinary, and other anthropogenic applications. As consumers are increasingly
informed about health, food, and nutrition issues, they are also realizing the poten-
tial and benefits of aromatic and medicinal plants and their metabolites. There are
many secondary metabolites which are produced by these plants; essential oils
(EOs) are among them. Composition of essential oils is very complex. Individual
components present in essential oils have valuable applications in various fields like
agriculture, environment, and human health. Essential oils are found as effective
complements to synthetic compounds which are used in the chemical industry.
The term essential oil dates back to the sixteenth century and derives from the
drug Quinta Essentia, named by Paracelsus von Hohenheim of Switzerland
(Brenner 1993). Essential oils (EOs) get their name because of their flammable
characteristics. According to French Agency for Normalization: Agence Française
It is challenging to find when first essential oil was extracted; actually ancient writ-
ings which tell about the medicinal distilled waters don’t exactly describe the pro-
cedure used. The very first document describes the distillation process dating back
to the ninth century when the Arabs brought essential oils (EOs) into Europe. In the
sixteenth century, the concept of essential oils and fatty oils, as well as methods for
the separation of essences from the aromatic waters, became well known. At that
time, EOs were commercialized with industrial, therapeutic, and cosmetic objec-
tives. By the end of the nineteenth century, chemists managed to isolate, separate,
and reproduce the active molecules of essential oils in perfumery, therapy, and other
industries.
There are more than 200 components present in the mixture of pure essential oils.
Normally, these mixtures contain phenylpropanic derivatives or terpenes (have min-
imal structural and chemical differences) (Rao and Pandey 2007). They can be cat-
egorized into two classes:
• Volatile fraction: Volatile fraction has 90–95% of total oil weight. It contains
monoterpenes, sesquiterpenes, and their oxygenated derivatives. Aliphatic alco-
hols, esters, and aldehydes may also be present in volatile fraction.
• Nonvolatile residue: Nonvolatile residue is 1–10% of total essential oil in weight.
It contains fatty acids, hydrocarbons, sterols, waxes, flavonoids, and
carotenoids.
1.4.1 Hydrocarbon
Essential oils contain chemical compounds that have carbon and hydrogen as their
building blocks. Isoprene is the major basic hydrocarbon unit found in essential oils.
Chemical structure of isoprene is as given below:
1.4.2 Terpenes
1.4.3 Alcohols
1.4.4 Aldehydes
1.4.5 Acids
Acids are anti-inflammatory in nature. In essential oils, organic acids are present in
very small quantity in free form. Plant acids act as components or buffer systems to
control acidity. For example, benzoic and cinnamic acids are present in benzoin.
1.4.6 Esters
Esters present in essential oil have soothing and balancing effects. Esters are effec-
tive antimicrobial agents due to the presence of alcohol in their structure. In medical
field, esters are characterized as sedative and antifungal, with balancing action on
nervous system. Some common esters present in essential oils are linalyl acetate in
the lavender and bergamot and geranyl formate in the geranium.
1.4.7 Ketones
generally (not always) toxic in nature. The most toxic ketone is thujone that is found
in sage, mugwort, tansy wormwood, and thuja oils. Other toxic ketones found in
EOs are pinocamphone in hyssops and pulegone in pennyroyal. Some nontoxic
ketones are fenchone in fennel essential oil, jasmone in jasmine essential oil, men-
thone in peppermint oil, and carvone in spearmint.
1.4.8 Lactones
1.5.1 Maceration
Maceration in fact produces more of “infused oil” rather than that of “essential oil.”
In this technique, plant material is soaked in the vegetable oil and then heated and
strained at a point on which produced product can be used for the massage
purpose.
Cold pressing is a technique used for the extraction of essential oils from the citrus
rinds like lemon, orange, bergamot, and grapefruit. This method encompasses the
simple rind pressing followed by the separation of rinds from the fruit, chopping,
and then pressing. As a result, a watery mixture is produced that contains both
essential oil and liquid present in the source material. These are separated from each
other by using appropriate method. It is significant to note that essential oils pro-
duced from this method have short shelf life as compared to other methods.
In solvent extraction, essential oil is extracted from plant material using a suitable
solvent. Generally, hydrocarbons are added as solvent into the plant material for the
extraction of essential oils. After the addition of solvent into the plant material, the
8 M. A. Hanif et al.
produced solution is filtered and then concentrated by the process of distillation. Oil
is extracted from the concentrate by the addition of pure alcohol which is then
evaporated, and oil is left behind. The main drawback of using this method is that
solvent residue left behind may cause allergies and also affect the immune system.
1.5.4 Enfleurage
Enfleurage is the traditional and intensive method for the extraction of essential oils
from the flowers. In this process, fat is layered over the flower petal for the extrac-
tion purpose. After the absorbance of essential oils by fat from the flower petals,
alcohol is used for the separation and extraction of essential oils from fat. At the end
of the process, pure essential oil is collected by evaporating the alcohol.
1.5.5 Hydrodistillation
Hydrodistillation has become obsolete for the essential oil extraction process. The
use of hydrodistillation in the developed countries is limited due to the production
of essential oils with burnt smell. As in this process, material is overheated which
causes the burning of aromatic compounds that result in the production of desired
product (essential oils) with burnt smell. This process seems to be effective for pow-
ders such as groundwood, spice powders, etc. and for tough materials such as nuts,
wood, or roots.
Turbo distillation process is appropriate for the extraction of coarse and hard plant
material like roots, seeds, and bark. In this process, plant material is soaked into the
water, and then steam is circulated through the plant material and mixture of water.
Throughout the process, same water is recycled through the plant material. This
method allows essential oil at a faster rate from the hard-to-extract plant materials.
Most commonly used technique for the extraction of the essential oil from the plant
material is called distillation. In this type of distillation, flowers or plants are placed
on screen, and steam passed through the material. Later steam is condensed to pro-
duce water and essential oil. At the end, this mixture of essential oil and water is
separated (Cassel et al. 2009).
Qualification and quantification of produced EOs are necessary to ensure its good
quality. Different classical as well as modern analytical techniques are used for the
analysis of produced EOs.
The earliest analytical techniques used for the examination of essential oils (EOs)
were generally focused on the quality aspects that concern only two main proper-
ties, i.e., purity and identity (Marques et al. 2009). Titrimetry and gravimetry are
classical analytical techniques that are used for the analysis of essential oils
(Marques et al. 2009; Guenther 2013). Specific gravity (SG) method is frequently
used for the investigation of physicochemical properties of EOs. Furthermore, clas-
sical methodologies have been also widely used for the analysis of chemical proper-
ties of essential oils (Guenther 2013).
Most of the analytical methods applied for the analysis of EOs are based on the
chromatographic procedures that help in the component identification as well as its
separation. However, other methods are also required for the confirmation to get
10 M. A. Hanif et al.
and parsley are characterized by most vital antioxidant properties (Aruoma 1998).
Most active compounds which show antioxidant properties are carvacrol and thy-
mol. Activity of these compounds is related to their phenolic structure. Due to the
redox properties of the phenolic compounds, they play a vital role in neutralization
of free radicals and also in decomposition of peroxides (Burt 2004). The antioxidant
activity of EOs is also due to other compounds present in essential oils like alcohols,
ketones, aldehydes, ethers, and monoterpenes. Common examples of these com-
pounds are linalool, geranial/neral, 1,8-cineole, isomenthone, menthone, citronellal,
α-terpinolene, α-terpinene, and β-terpinene (Aruoma 1998).
Essential oils show potential activity for the treatment of cancer. Essential oils con-
tain anticancer natural products (Edris 2007) which play a vital role in the preven-
tion and recovery from cancer. There are certain foods like turmeric and garlic
which are considered to be good sources of the anticancer agents (Edris 2007).
Essential oil obtained from garlic has sulfur compounds like diallyl trisulfide, dial-
lyl sulfide, and diallyl disulfide which show preventive effect against cancer (Milner
2001, 2006).
12 M. A. Hanif et al.
1.7.5 Cytotoxicity
There are no specific cellular ligands found in essential oils due to their complex
chemical composition (Carson and Riley 1995). As lipophilic mixtures, EOs have
an ability to degrade cell membrane layers of phospholipids, fatty acids, and poly-
saccharides. Furthermore, EOs may coagulate cytoplasm (Lambert et al. 2001) and
also damage proteins and lipids present in cytoplasm (Ultee et al. 2002; Burt 2004).
Damage to the wall and the cell membrane can lead to the leakage of macromole-
cules and lysis (Turina et al. 2006). Increase in the membrane permeability leads to
the death of the cell by the process of necrosis and apoptosis (Oussalah et al. 2006;
Novgorodov and Gudz 1996).
Essential oils have various structurally diverse chemical compounds with a variety
of repellent and insecticidal mechanisms. There are several factors that affect the
commercialization of essential oils. These include biological activity, intellectual
property value, product quality, regulatory requirements, and product performance
(Ahmed and Eapen 1986). The EOs have toxic effect for both granary insects and
flying insects. Eucalyptus (Myrtaceae) and Gaultheria (Ericaceae) oils showed very
high toxic effect to kill insects (Mateeva and Karov 1983). Generally, EOs can be
ingested, inhaled, or absorbed by the skin of insects. EOs also show fumigant toxic-
ity (Regnault-Roger and Hamraoui 1995). For example, Anopheles funestus
(Culicidae: Diptera), Pediculus capitis (Pediculidae: Anoplura), Periplaneta orien-
talis (Dictyoptera: Blattidae), and Cimex lectularius (Cimicidae: Hemiptera) are
killed by the use of essential oils obtained from Eucalyptus saligna (Myrtaceae)
within 2–30 min.
1 Essential Oils 13
Essential oils have an important part in the medical field due to their extraordinary
medicinal properties. Several EOs show fungicidal, antidepressant, antibacterial,
stimulating, and relaxant effect and can be used as an effective therapeutic agent. As
essential oils exhibited remarkable therapeutic properties, that is why these oils are
used effectively for the treatment of several infections caused by either pathogenic
or nonpathogenic diseases. Pathogenic diseases caused by virus, fungi, and bacteria
can be treated with the use of respective essential oils. Nonpathogenic diseases are
also treated with the appropriate use of essential oils. For example, essential oil
obtained from garlic significantly showed lowering in serum cholesterol and triglyc-
erides (TGs) by raising the level of lipoproteins (high density) in patients with coro-
nary heart diseases (Bordia 1981). Some EOs possess hypotensive activity and are
used for the treatment of hypertension. EOs and their individual aroma constituents
showed anti-cancerous properties and are used in the treatment of breast cancer,
tumors, leukemia, glioma, and many others. Sesquiterpene hydrocarbon elements
present in EOs in very small amounts are effective for the treatment of glioma
(malignant human tumors) (DeAngelis 2001). Antiangiogenic therapy is considered
to be one of the most promising methodologies to control cancer.
There are various EOs like citronella oil which are used as insecticides or as insect
repellents and in veterinary applications. After the ban on the usage of antibiotics in
the feed of animals, EOs have emerged as a potential alternative to antibiotics used
in the feed of animals. EOs used in veterinary field are categorized into the follow-
ing classes:
1 . Essential oils which attract animals
2. Essential oils which repel animals
3. Antiparasitic, pest repellent, and insecticidal essential oils
4. Essential oils used in the feed of animals
5. Essential oils used for the treatment of animal disease/s
Essential oils are used in the feed of animals as an enhancer for pancreatic and gas-
tric juice production, stimulant for the production of saliva, appetite stimulant, and
antioxidant and antimicrobial for the improvement of broiler performance. EOs due
to their effective nature should be used in minute quantities in animal nutrition.
Otherwise, they can cause reduction in feed intake, accumulation in the animal tis-
sues, and disturbance in gastrointestinal microflora. Taste and odor of EOs may
contribute to the refusal of feed by the animals, but encapsulation of EOs is the
14 M. A. Hanif et al.
solution of this problem (Baser and Franz 2010). Generally, essential oils used in
the treatment of the human diseases are also recommended for the treatment of
animal diseases.
1.8.3 Aromatherapy
For many, the word “aromatherapy” originally became related to the idea of the
holistic use of EOs for promoting the health and well-being. With the passage of
time, the psychophysiological effects of EOs have been explored continuously. The
use of EOs to aid sedation and to reduce anxiety is also discussed in aromatherapy.
More significantly, practice of the aromatherapy is firmly related with inhalation of
EOs in small doses and their applications to the skin in highly diluted form as a part
of aromatherapy massage. Aromatherapy is among the complementary therapies
which are used for the treatment of many diseases with the use of EOs as major
therapeutic agents. Inhalation, baths, and local applications are the major approaches
used in “aromatherapy” that utilize EOs to penetrate into the surface of the human
skin with the marked aura. After the entrance of EOs in system, they re-modulated
themselves and work in a friendly manner at affected area or at malfunction site.
Aromatherapy uses several combinations and permutation to get relief from several
ailments like indigestion, depression, insomnia, headache, respiratory problems,
muscular pain, urine-associated complications, swollen joints, skin ailments, etc.
The use of EOs is found to be more favorable when other facets of life and diet are
made due consideration.
The use of essential oils (EOs) at industrial level is a very promising area for the
development of any country. The quick development of flavor and fragrance indus-
try in the nineteenth century was largely based on the EOs and related other natural
products. In 1876, Haarman and Reimer started to synthesize vanillin (synthetic
aroma chemicals) and then anisaldehyde, coumarin, terpineol, and heliotropin.
1 Essential Oils 15
Even though aroma chemicals made revolution in flavors and fragrances with top
discoveries in the twentieth century, for several decades both fragrances and flavors
were synthesized with elements of natural origin, nearly all of which were EOs.
Essential oils have the ability to promote wellness when they are used as a part of
healthy lifestyle. Independently, EOs have various benefits for human body. When
the use of EOs is combined with the physical activities and proper eating manner,
they helped the user to feel better overall. The beauty of EOs is that they may be
tailored to any type of workout by the alternation in the application methods and EO
types to fit the preference and needs of the users. During routine exercise (heavy
lifting, dusty hiking trail, intense cardio and recreational sports), EOs can be used to
keep the body at peak performance. Essential oils are also a healthy part of weight
loss program when their use is combined with the healthy eating and consistent
exercise.
Essential oils (EOs) have very concentrated properties of the plant or herb from
which they are derived. A very small amount of the EOs often have the qualities of
several cups of herbal tea from the same plant. As an example, one drop of pepper-
mint EO is comparable to 26–28 cups of the peppermint tea. This is not to say EOs
shouldn’t be used, but these oils should be utilized with great care and in safe
amounts. However, there are several essential oils which are not safe to use inter-
nally, and others should really be used with great caution. As EOs are the equivalent
to 10–50 cups of herbal tea (depends on the used herb) or 20× the suggested dose of
herbal tincture of the exact same herb, they need to only be taken internally in cir-
cumstances where they are completely needed and with great care. However, there
are many warnings about the safe utilization of EOs. EOs are excellent natural rem-
edies when used in a proper way.
References
Ahmed S, Eapen M (1986) Vapour toxicity and repellency of some essential oils to insect pests.
Indian Perfumer 30:273–278
Aruoma OI (1998) Free radicals, oxidative stress, and antioxidants in human health and disease.
J Am Oil Chem Soc 75:199–212
Baser KHC, Franz C (2010) Essential oils used in veterinary medicine. In: Baser KHC (ed)
Handbook of essential oils. CRC Press, Boca Raton, pp 881–894
16 M. A. Hanif et al.
Bordia A (1981) Effect of garlic on blood lipids in patients with coronary heart disease. Am J Clin
Nutr 34:2100–2103
Brenner DM (1993) Perilla: botany, uses and genetic resources. In: Janick J, Simon JE (eds) New
crops. Wiley, New York, pp 322–328
Burt S (2004) Essential oils: their antibacterial properties and potential applications in foods—a
review. Int J Food Microbiol 94:223–253
Carson C, Riley T (1995) Antimicrobial activity of the major components of the essential oil of
Melaleuca alternifolia. J Appl Bacteriol 78:264–269
Cassel E, Vargas R, Martinez N, Lorenzo D, Dellacassa E (2009) Steam distillation modeling for
essential oil extraction process. Ind Crop Prod 29:171–176
Cox S, Mann C, Markham J, Bell H, Gustafson J, Warmington J, Wyllie S (2000) The mode of
antimicrobial action of the essential oil of Melaleuca alternifolia (tea tree oil). J Appl Microbiol
88:170–175
Dale D, Saradamma K (1981) Insect antifeedant of some essential oils. Pesticides 15:21–22
DeAngelis LM (2001) Brain tumors. N Engl J Med 344:114–123
Denyer S (1991) Biocide-induced damage to the bacterial cytoplasmic membrane. In: Mechanisms
of action of chemical biocides. Blackwell Scientific Publications, Oxford/Boston, pp 171–188
Dorman H, Deans SG (2000) Antimicrobial agents from plants: antibacterial activity of plant vola-
tile oils. J Appl Microbiol 88:308–316
Edris AE (2007) Pharmaceutical and therapeutic potentials of essential oils and their individual
volatile constituents: a review. Phytother Res 21:308–323
Guenther E (2013) The essential oils-vol 1: history-origin in plants-production-analysis. Read
Books Ltd, New York
Gustafson J, Liew YC, Chew S, Markham J, Bell HC, Wyllie SG, Warmington J (1998) Effects of
tea tree oil on Escherichia coli. Lett Appl Microbiol 26:194–198
Isman MB, Koul O, Luczynski A, Kaminski J (1990) Insecticidal and antifeedant bioactivities of
neem oils and their relationship to azadirachtin content. J Agric Food Chem 38:1406–1411
Knobloch K, Weigand H, Weis N, Schwarm H, Vigenschow H (1986) Action of terpenoids on
energy metabolism. Walter de Gruyter, Berlin, Germany
Koh K, Pearce A, Marshman G, Finlay-Jones J, Hart P (2002) Tea tree oil reduces histamine-
induced skin inflammation. Br J Dermatol 147:1212–1217
Lambert R, Skandamis PN, Coote PJ, Nychas GJ (2001) A study of the minimum inhibitory con-
centration and mode of action of oregano essential oil, thymol and carvacrol. J Appl Microbiol
91:453–462
Marques CA, Leitão GG, Bizzo HR, Peixoto AL, Vieira RC (2009) Anatomy and essential oil
analysis of the leaves from Hennecartia omphalandra J. Poisson (Monimiaceae). Rev Bras
19:95–105
Maruyama N, Sekimoto Y, Ishibashi H, Inouye S, Oshima H, Yamaguchi H, Abe S (2005)
Suppression of neutrophil accumulation in mice by cutaneous application of geranium essen-
tial oil. J Inflamm 2:1
Mateeva A, Karov S (1983) Studies on the insecticidal effect of some essential oils. Nauchni
Trudove-Vissh Selskost Inst Vasil Kolarov 28:129–139
Milner JA (2001) A historical perspective on garlic and cancer. J Nutr 131:1027S–1031S
Milner JA (2006) Preclinical perspectives on garlic and cancer. J Nutr 136:827S–831S
Moon T, Wilkinson JM, Cavanagh HM (2006) Antiparasitic activity of two Lavandula essential
oils against Giardia duodenalis, Trichomonas vaginalis and Hexamita inflata. Parasitol Res
99:722–728
Novgorodov SA, Gudz TI (1996) Permeability transition pore of the inner mitochondrial membrane
can operate in two open states with different selectivities. J Bioenerg Biomembr 28:139–146
Oussalah M, Caillet S, Lacroix M (2006) Mechanism of action of Spanish oregano, Chinese cin-
namon, and savory essential oils against cell membranes and walls of Escherichia coli O157:
H7 and Listeria monocytogenes. J Food Prot 69:1046–1055
Pauli A (2001) Antimicrobial properties of essential oil constituents. Int J Aromather 11:126–133
1 Essential Oils 17
Rao VPS, Pandey D (2007). A project report on Extraction of essential oil and its applications
for Bachelor of Technology (Chemical Engineering) at Department of Chemical Engineering
National Institute of Technology Rourkela-769008 Orissa, India
Regnault-Roger C (1997) The potential of botanical essential oils for insect pest control. Integr
Pest Manag Rev 2:25–34
Regnault-Roger C, Hamraoui A (1995) Fumigant toxic activity and reproductive inhibition
induced by monoterpenes on Acanthoscelides obtectus (Say)(Coleoptera), a bruchid of kidney
bean (Phaseolus vulgaris L.). J Stored Prod Res 31:291–299
Reverchon E (1997) Supercritical fluid extraction and fractionation of essential oils and related
products. J Supercrit Fluids 10:1–37
Rim I-S, Jee C-H (2006) Acaricidal effects of herb essential oils against Dermatophagoides farinae
and D. pteronyssinus (Acari: Pyroglyphidae) and qualitative analysis of a herb Mentha pule-
gium (pennyroyal). Korean J Parasitol 44:133
Sikkema J, de Bont JA, Poolman B (1994) Interactions of cyclic hydrocarbons with biological
membranes. J Biol Chem 269:8022–8028
Turina ADV, Nolan M, Zygadlo J, Perillo M (2006) Natural terpenes: self-assembly and membrane
partitioning. Biophys Chem 122:101–113
Ultee A, Bennik M, Moezelaar R (2002) The phenolic hydroxyl group of carvacrol is essential for
action against the food-borne pathogen Bacillus cereus. Appl Environ Microbiol 68:1561–1568
Yoon HS, Moon SC, Kim ND, Park BS, Jeong MH, Yoo YH (2000) Genistein induces apoptosis
of RPE-J cells by opening mitochondrial PTP. Biochem Biophys Res Commun 276:151–156
Zellner BDA, Dugo P, Dugo G, Mondello L (2010) Analysis of essential oils. In: Handbook of
essential oils. CRC Press, Taylor and Francis Group, London, pp 151–184
Chapter 2
Factors Influencing the Production
and Chemical Composition of Essential
Oils in Aromatic Plants from Brazil
Carmen Sílvia Fernandes Boaro, Maria Aparecida Ribeiro Vieira,
Felipe Girotto Campos, Gisela Ferreira, Iván De-la-Cruz-Chacón,
and Márcia Ortiz Mayo Marques
2.1 Introduction
such as light, water, temperature, nutrients, types of soils, pH, and altitude, among
others (Amdouni et al. 2016; Morshedloo et al. 2017; Verpoorte 2000).
Some plants when attack by herbivores, release mixtures of volatile com-
pounds, which attract organisms that feed on the parasites, thereby reducing the
damage and benefiting the plant (Degenhardt et al. 2009; Duarte et al. 2010;
Jamieson et al. 2017).
Biotic factors, related to plant species, and abiotic factors, related to the environ-
ment, influence the production and chemical composition of essential oils (Aslam
et al. 2017; Shao et al. 2007; Silvas et al. 2013).
Among the many species distributed in several families with medicinal and aro-
matic potentials, we will highlight in this chapter some belonging to the families
Lamiaceae (Mentha x piperita L., Ocimum selloi Benth, reclassified as Ocimum
carnosum (Spreng.) Link & Otto ex Benth, Ocimum basilicum L., Origanum vul-
gare L., and Thymus vulgaris L.), Asteraceae (Lychnophora ericoides Mart.,
Lychnophora pinaster Mart., and Baccharis dracunculifolia DC), and Boraginaceae
Varronia curassavica Jacq (synonym: Cordia verbenaceae DC) (Bueno 2004;
Scavroni et al. 2005; Valmorbida et al. 2006; De Fazio 2007; David 2007; Vasques
2007; De Fazio 2011; Carboni 2013; Facanali et al. 2015; Búfalo 2015; Bolina
2015; Búfalo et al. 2016; Haber 2008; Isobe 2012; Silva 2013; Vieira et al. 2014;
Silva 2016; Belini 2015).
Medicinal and aromatic plants are plant species that have one or a group of sub-
stances that have biological activities, such as insecticidal, larvicidal, anticancer,
antifungal, anti-inflammatory, analgesic, antiemetic, antimalarial, carminative,
stimulant, antispasmodic, antiulcer, antimicrobial and antirheumatic, among others
(Park et al. 2009; Abdelwahab et al. 2010; Rana et al. 2011; Stojkovic et al. 2011;
Millezi et al. 2012; Millezi et al. 2013; Oliveira et al. 2012; Oliveira et al. 2013;
Aznar et al. 2015). Many species that exhibit intense odor due to the release of
volatile substances are classified as aromatic plants and may not be medicinal.
Since ancient times, medicinal and aromatic plants have been used as culinary
spices to enhancing the organoleptic properties of foods (Bozin et al. 2006); as natu-
ral medicines, especially due to their antimicrobial properties (Dorman and Deans
2000); and as an alternative to the use of synthetic chemicals in agriculture (Antunes
and Cavaco 2010).
The family Lamiaceae has approximately 245 genera and 7886 species. Among
these, 46 genera and 525 species have been identified in Brazil (Harley et al. 2015).
Many of these genera were brought by the colonizers and acclimated easily, being
grown in gardens and vegetable gardens, such as Mentha (mint), Ocimum (alfavaca),
Origanum (oregano), Rosmarinus (rosemary), and Salvia (salvia) (Joly 1993).
Mentha x piperita L., known as peppermint and mint, is widely grown and con-
tains essential oils used in the pharmaceutical, alcoholic beverage, food, and cos-
22 C. S. F. Boaro et al.
metic industries (Gupta 1991; Munsi 1992). Among the mints, Mentha x piperita L.
presents the higher content of menthol (Loewenfeld and Back 1980), a substance
with important biological activities (Table 2.1). It is an annual or perennial aromatic
plant, semi-erect, and about 30 cm high. Its branches range from dark green to
purplish purple, and its leaves are elliptic-acuminate, jagged, and pubescent
(Goutham 1980; Simões and Spitzer 2000; Lorenzi and Matos 2008).
Table 2.1 Biological activity of essential oil or its volatile substances isolated from aromatic
species
Popular Substance/ Biological
Scientific name name essential oil activity(ies) Reference(s)
Mentha x Mint Menthol Analgesic, Stengel et al. (2007);
piperita antifungal, Edris and Farrag (2003);
antibacterial, and Trombetta et al. (2005);
anticancer Monteith et al. (2007);
Kim et al. (2012); Wang
et al. (2012); Li et al.
(2009)
Essential oil Antifungal, Carreto (2010); Kumar
larvicidal, and et al. (2011); Sousa et al.
antispasmodic (2010)
Mentha x Creeping Essential oil and Antischistosomal Matos-Rocha et al.
villosa mint rotundifolone (2013), (2017)
Ocimum selloi Basil Essential oil Anticancer Cola et al. (2003)
Ocimum Sweet Essential oil Antibacterial Valeriano et al. (2012)
basilicum basil
Ocimum Sweet Essential oil Antifungal Saggiorato et al. (2012)
basilicum basil
Origanum Oregano Essential oil Antibacterial Cattelan (2015)
vulgare
Thymus Thyme Thymol Antibacterial and Giordani et al. (2004);
vulgaris antifungal Klaric et al. (2007)
Lychnophora Arnica Essential oil and Anti-inflammatory Pavarini et al. (2013)
ericoides ortho-acetoxy- and analgesic
bisabolol
Essential oil Acaricidal Baldin et al. (2010)
Lychnophora Arnica Essential oil Antibacterial Queiroz (2012)
pinaster
Baccharis Rosemary Essential oil Antiviral Sforcin et al. (2012)
dracunculifolia
Varronia Erva trans- Anti-inflammatory Fernandes et al. (2007)
curassavica baleeira Caryophyllene
α-Humulene
Psidium Brazilian Essential oil Antioxidant, Nascimento et al. (2017)
guineense guava antiproliferative,
Spathulenol
and
anti-inflammatory
Turnera Chanana Essential oil Antibacterial Fernandes et al. (2014)
subulata
2 Factors Influencing the Production and Chemical Composition of Essential Oils… 23
Ocimum selloi Benth, reclassified as Ocimum carnosum (Spreng.) Link & Otto
ex Benth, a native species occurring in the southeastern and southern regions of
Brazil, is herbaceous, perennial, and up to 1.20 m tall and blossoms throughout the
year. The inflorescence is a terminal spike with purple flowers (Morhy 1973). It is
popularly known in the states of Rio de Janeiro and Espírito Santo as paregoric
elixir, in Minas Gerais as anis and alfavaquinha, and in São Paulo as atroveran
(Martins et al. 1997). The species is used in popular medicine as a digestive and for
treatment of gastritis, vomiting, cough, and bronchitis. Pre-clinical tests (Vanderlinde
et al. 1994) refer to its antidiarrheal, antispasmodic, and anti-inflammatory properties
(Table 2.1).
Ocimum basilicum L. known as basil, alfavaca, great basil, and Saint-Joseph’s
wort is cultivated in many countries, representing a source of raw material (extracts
and essential oils) for the industries that produce cosmetics, perfumes, pesticides,
pharmaceuticals, and food (Umerie et al. 1998; Keita et al. 2001; Pascual-Villalobos
and Ballesta-Acosta 2003; Prasad et al. 1986; Hussain et al. 2008). In popular medi-
cine, the species is used due to its carminative, stimulating, and antispasmodic prop-
erties (Marotti et al. 1996). Basil is an annual herb, used in culinary preparations in
Mediterranean countries (Sifola and Barbieri 2006), native to India and other
regions of Asia (Klimankova et al. 2008), presenting purple or white flowers.
According to the aroma, basil may be classified as sweet, lemon, cinnamon, cam-
phor, anise, and clove (Blank et al. 2004). Basil plants are also used for ornamental,
medicinal, and aromatic purposes, its essential oil being valued in the international
market due to the high content of the compound linalool (Blank et al. 2004). Its
leaves are used dry or fresh, such as flavoring in foods, confectionery, and beverages
(Kopsell et al. 2005).
Some of the essential oil components, such as eucalyptol, linalool, and camphor,
are known to have biological activity (Morris et al. 1979), such as antibacterial
(Elgayyar et al. 2001) and insecticidal (Bowers and Nishida 1980).
Oregano (Origanum vulgare L.) is native to the mountainous regions of Southern
Europe and grown in Brazil as a spice used in culinary. The plant is used in home
medicine, and its essential oil is used in the composition of food flavorings and
perfumes (Lorenzi and Matos 2008). Studies of the subspecies Origanum vulgare L.
ssp. vulgare, very widespread in Italy (Ietswaart 1980), describe essential oils ger-
macrene D, β-ocimene, β-caryophyllene, and sabinene as essential substances
(Mockute et al. 2001; Mockute et al. 2003), whose biological activities are shown in
Table 2.1.
Thymus vulgaris L., a shrub that grows up to 50 cm high, has white or purple
flowers and is an aromatic species, which has a slightly bitter taste and is popu-
larly known as thyme or common thyme. Infusion of leaves and floral buds are
used in popular medicine. Flowers and dried plants are used as tonic, emmena-
gogue, antispasmodic, antiseptic, antiparasitic, sleep inducer and relief of head-
ache (Naghibi et al. 2005; Figueiredo et al. 2008). The major substances of its
essential oil are thymol, p-cymene, carvacrol, 1,8-cineole, borneol, and linalool.
Thymol is a phenolic monoterpene that has several pharmacological properties
(Table 2.1), including antibacterial and antifungal properties (Giordani et al.
24 C. S. F. Boaro et al.
2004; Klaric et al. 2007, Braga et al. 2007; Ahmad et al. 2011; Sienkiewicz
et al. 2012).
Asteraceae, the largest family of eudicotyledons, presents 1911 genera, with
3293 species (Nakajima et al. 2015). In Brazil, its species represent the equivalent
of 10% of vascular plants. They are commonly found in open fields, being uncom-
mon in humid tropical forests (Funk et al. 2005). They have high adaptability to the
most diverse habitats and climatic conditions, and their sizes vary from sub-shrubs,
annual, or perennial grass to shrub or liana. Inflorescence of a flower head type and
fruit of cypsela type, also called achene, are its main characteristics (Funk et al.
2005; Canceli et al. 2007; Heiden et al. 2007; Souza and Lorenzi 2008).
Lychnophora ericoides Mart. and Lychnophora pinaster Mart. are endemic aro-
matic and medicinal species in Brazil, presenting near-morphological characteris-
tics, making it difficult to distinguish between them (Semir 1991; Semir et al. 2011).
Due to the polymorphism and overlays of characters, Coile and Jones (1981) syn-
onymized L. pinaster and L. ericoides. L. pinaster shows polymorphism in the size,
diameter of the branches, shape, length, and width of the leaves. Although L. pinas-
ter usually presents smaller individuals, with sub-bush size and thinner branches, it
may also present individuals with similar dimensions to L. ericoides, who show a
generally larger size and more robust branches. The species present medicinal
potential, being used in popular medicine as anti-inflammatory and analgesic
(Table 2.1).
Lychnophora ericoides, popularly known as arnica, false arnica, or candle, blos-
soms and fruits throughout the year, with reproductive periods that may vary
among populations. L. ericoides occurs in sites with altitudes between 950 m and
1800 m, in the states of Minas Gerais and Goiás. In the Minas Gerais it is distrib-
uted along of the Serra do Espinhaço, Planalto de Diamantina, Furnas, Serra da
Canastra and in some places in southeast of state. In Goiás occurs in Cristalina city,
Chapada dos Veadeiros, Serra Dourada, Serra dos Pireneus, and Serras near Brasília
(Semir et al. 2011).
Lychnophora pinaster Mart., also popularly known as arnica, may be found in
rocky, dirty, and clean fields (Rodrigues 1988). In addition, it also occurs in canga
fields, between blocks of rocks or on top of small hills exposed to intense sunshine,
and in xeric environments, where winters are humid and cold and summers are dry
and warm (Semir 1991). The species is common in southeast of Serra do Espinhaço,
in the state of Minas Gerais, southeast region of Brazil (Flora do Brasil 2020).
Baccharis dracunculifolia DC, also known as rosemary, field rosemary, and field
broom, occurs in Brazil, Paraguay, Argentina, Uruguay, and in the high valleys of
Bolivia, reaching up to 3280 m in altitude (Cassel et al. 2000). Woody shrub, mea-
suring up to 4.0 m high, presents rapid growth and occurs in southeast, central-west,
and southern regions of Brazil (Nakajima et al. 2015). The origin of one of its popu-
lar names is due to the fact that the species has been very used in the making of
rustic brooms. It is also used as an ornamental plant, being important for the local
population for its use in home medicine to mainly combat gastric disorders, physi-
cal fatigue, inappetence, febrile affections, and organic weakness (Carneiro and
Fernandes 1996; Mors et al. 2000). The essential oil extracted from the field rosemary
2 Factors Influencing the Production and Chemical Composition of Essential Oils… 25
leaves is valued in the fragrance industry (Molt and Trka 1983), due to the high
content of trans-nerolidol (Table 2.1), and is also the main botanical source for the
production of green propolis in southeast Brazil (Park et al. 2004). The species
should be highlighted in the regeneration of natural vegetation after disturbances,
such as fire (Tabarelli and Mantovani 1999; Galindez et al. 2009).
The family Boraginaceae presents 4 subfamilies, namely Ehretioideae,
Cordioideae, Helitropioideae, and Boraginoideae (Miller and Gottschling 2007),
130 genera, and 2500 species (Al-Shehbaz 1991), distributed in tropical, subtropi-
cal, and temperate regions, being rare in the temperate zones of the Northern
Hemisphere (Al-Shehbaz 1991). It was from this family the production of the first
Brazilian commercial herbal medicine with anti-inflammatory activity (Table 2.1)
from the essential oil of Varronia curassavica Jacq (synonymy: Cordia verbenaceae
DC), medicinal plant native to Brazil, which occurs in coastal regions (Vaz et al.
2006; Passos et al. 2007), from Amazon region (Akisue et al. 1983) to Rio Grande
do Sul (Montanari Jr 2000).
It is popularly known as erva baleeira, salicina, catinga-de-barão, cordia, erva-
balieira, balieira-cambará, erva-preta, maria-milagrosa, maria-preta, catinga-
preta, maria-rezadeira, camarinha, camaramoneira-do-brejo, and pimenteira
(Montanari Jr 2000; Carvalho Júnior et al. 2004; Lorenzi and Matos 2008). Other
botanical synonyms are Cordia curassavica, Cordia salicina DC., Cordia cylindro-
stachya, Lithocardium fresenii, Lithocardium salicinum, and Lithocardium verba-
ceum (Carvalho Júnior et al. 2004). The aerial part of the species has a strong and
persistent odor (Passos et al. 2007). Standing bush, very branched, with the end of
the hanging branches and stems covered by fibrous bark, the plant reaches between
1.5 m and 2.5 m high. It has simple, alternating, coriaceous, aromatic leaves that are
5 to 9 cm in length (Lorenzi and Matos 2008). It is commonly used in the form of
alcoholic extracts, decoctions, and infusions due to its antiulcer, antimicrobial,
anti-inflammatory, antirheumatic, analgesic, and tonic properties (Akisue et al.
1983; Vaz et al. 2006; Medeiros et al. 2007; Passos et al. 2007).
The medicinal property of the species is due to the presence of α-humulene in its
essential oil, a sesquiterpene substance that presents anti-inflammatory action
(Montanari Jr 2000).
Excluding the extraction of essential oils from citrus fruits, the main methods of
extraction of essential oils are steam distillation and hydrodistillation, which con-
sist of the vaporization of essential oil, which is drawn together with steam into a
condenser, where it is cooled, returns to the liquid phase, and is stored in a separa-
tor vessel.
In the process steam distillation, the steam is generated either in a boiler sepa-
rated from the vegetal material. The water vapor stream generated is passaged in
matriz vegetal and the mixture of water and essential oils is condenses and collected
26 C. S. F. Boaro et al.
The quantitative diversity, expressed by yield, and the qualitative diversity, related
to the chemical composition of essential oils, may be influenced by genetic and
epigenetic (Gupta et al. 2016; Pandotra et al. 2013; Trapp and Croteau 2001;
Richards 2006; Bird 2007) and abiotic factors, including mineral nutrition, water,
light, temperature, and soil types, and biotic factors, such as attacks of pathogens,
pests, and herbivores. All these factors may act and increase or decrease both the
yield and the chemical composition of essential oils. In addition, the factors must be
considered together, characterizing the seasonality, potential causes of stresses that
may influence the primary metabolism, and, consequently, the specialized metabo-
lism (Amdouni et al. 2016; Figueiredo et al. 2007; Gouinguené and Turlings 2002;
Kamanula et al. 2017; Lima et al. 2014; Ormeno and Fernandez 2012).
In this chapter, we will emphasize some of these factors that interfere with the
production and chemical composition of essential oils.
selection, or drift. The patterns of genetic diversity in populations are the result
of a variety of forces that act to eliminate or increase and disperse new mutant
alleles and chromosome rearrangements between individuals and populations
(Frankham et al. 2008).
The concern with the loss of variability and the extinction of species with poten-
tial biological activity, due to extractivism and deforestation among other causes,
has generated demand for works that allow the study of these species in order to
preserve them (Karp et al. 1997).
The study of population genetics allows the knowledge of the distribution, struc-
ture, and genetic diversity between and within populations, making possible the
choice of efficient strategies for the conservation of the biodiversity of the species.
In this context, molecular markers are important tools for the calculation of
population estimations (Allendorf et al. 2010; Haber 2008; Primack and Rodrigues
2001; Silva et al. 2013), allowing the evaluation of gene flow. These markers may
be simple sequence repeats (SSRs), random amplification of polymorphic DNA
(RAPD), amplified fragment length polymorphism (AFLP), and single nucleotide
polymorphisms (SNP) (Ferreira 2001; Milach 1998; Cho et al. 1999). In the
sequence, we will present studies involving population genetics and the essential
oils of native aromatic and medicinal species of the families Asteraceae
(Lychnophora pinaster Mart., Baccharis dracunculifolia DC.) and Lamiaceae
(Ocimum selloi Benth).
Haber (2008) characterized the chemical composition of essential oil and genetic
diversity of four native populations of Lychnophora pinaster collected in Cerrado in
the state of Minas Gerais, Brazil, from different geographical regions: Carrancas
city (populations: ERE and ERE1), Lavras (population: ANT) and between the cit-
ies of Lavras and Ingaí (population: PBO). The author noted hight genetic diversity
within populations (90.5%) and only 9.43% between them. The populations ERE,
ERE1 and PBO presented a similar chemical profile, the major substance being
methyl trans-cinnamate with a relative average abundance per population of 59.1%,
77.1%, and 79.7%, respectively. These populations differed from the population
ANT, whose main constituent was cedr-8(15)-en-9-alpha-ol (27.9%), with the pres-
ence of methyl trans-cinnamate, with a significantly lower content (12.5%) than
that observed for the other populations.The divergence in the chemical profile of
essential oil of the population ANT is a result of the response of the genotypes to
the environmental variations between the collection and cultivated regions.
Silva (2016) evaluated the characterization of genetic diversity patterns and pop-
ulation structure of seven other natural populations of Lychnophora pinaster, using
a microsatellite marker (SSR), being a population of occurrence in the southeast
region of the state of Minas Gerais, called Ouro Branco (OB), whose soil is rich in
iron ore, and six populations in the south region, called Poço Bonito (PB), Serra do
Sofá (SS), Serra da Arnica (SA), Serra do Salto (SSa), Serra Branca (SB), and Areia
Branca (AB), a region whose soil is rich in aluminum. The author carried out a
chemical characterization of the essential oils of the leaves of the populations
Ouro Branco (OB), Areia Branca (AB), and Serra do Salto (SSa). The results
revealed the existence of genetic diversity within and between populations, with
28 C. S. F. Boaro et al.
special divergence in genetic diversity among the population of the southeast region
(Ouro Branco) in relation to those occurring in the southern regions PB, SS, AS,
SSa, SB, and AB. The chemical composition of the essential oils between the popu-
lations was divergent. The essential oils of populations of southern regions pre-
sented methyl trans-cinnamate (phenylpropanoid), as the most abundant substance
(about 70% of essential oil), while in the essential oil of southeast region (OB), the
presence of metabolites belonging to the phenylpropanoid class was not detected,
with a greater abundance of sesquiterpenes (about 60% of essential oil). The
observed divergence in the chemical composition of essential oils may be attributed
to the genetic divergence and environmental conditions of the sites of occurrence of
L. pinaster.
Vieira et al. (2014) evaluated the structure and genetic diversity of native popula-
tions of Lychnophora collected in the State Minas Gerais, with microsatellite
marker (SSR). Three populations of Lychnophora ericoides were collected in the
cities of São Roque de Minas (population P1) and Capitólio (populations P2 and
P3). The geographic distance between the populations of L. ericoides was 80 km
between P1 and P2, 40 km between P2 and P3, and 120 km between P1 and P3. The
populations of L. ericoides showed a greater genetic diversity within than between
populations (62% and 37%, respectively) and showed that the populations have a
genetic structure (FST = 0,324), indicating a low gene flow among the populations
evaluated. The author carried out the chemical characterization of essential oils of
the leaves of the populations of São Roque de Minas (P1) and Capitólio (P2), with
distance at 80 km and with altitude at 1118 m and 933 m, respectively (Vieira et al.
2017). Sesquiterpenes were the main chemical class identified in the essential oil of
population P1 (São Roque de Minas), with the majority being spathulenol (mean
value of the population, 5.6%), γ-eudesmol (9.2%), epi-α-cadinol (8.6%),
α-muurolol (17.0%), and α-eudesmol (16.3%). On the other hand, essential oil of
population P2 (Capitólio) presented monoterpenes and sesquiterpenes, the main
substances being α-pinene (mean value, 4.6%), limonene (5.6%), terpinen-4-ol
(9.3%), β-atlantol (18.8%), and ortho-acetoxy-bisabolol (21.4%). The divergence
in the chemical composition of essential oils among the populations of L. ericoides
may be associated with the modulation of the biosynthesis of the specialized metab-
olites due to the interaction genotype-environment of the different regions of the
species occurrence.
The characterization of the chemical composition of essential oils and genetic
diversity using RAPD marker was made with three native populations of Ocimum
selloi occurring in contrasting climatic and altitude regions – the populations were
collected in the cities of Iporanga (PQ; altitude, 192 m), Piquete (VR; altitude,
908 m) in the state of São Paulo, and Adrianópolis (ADR; altitude, 247 m) in the
state of Paraná. The region VR was classified as Cwa (Köppen) with an average
temperature of 21.3 °C, annual rainfall of 1672.5 mm, and dry and cold winter
(average temperature of 11 °C), distinct from the two other regions (PQ and ADR)
classified as Af (Köppen), with a mean temperature of 23.6 °C, annual rainfall of
2033.8 mm, and winter with average temperature of 20 °C, without dry season. The
populations presented genetic divergence as a function of the geographical region,
with a lower diversity between the populations ADR and PQ, whose regions are
2 Factors Influencing the Production and Chemical Composition of Essential Oils… 29
geographically close, probably due to the occurrence of gene flow among the
accessions. The major substances of the population Piquete (PQ) were germacrene D,
elemicin, trans-α-bergamotene, and bicyclogermacrene. For the populations
Adrianópolis and Iporanga, the major substances were elemicin, β-selinene, and
β-4-copaen-α-ol. The results showed that the chemical composition of essential oils
was influenced by geographical region and genetic factor (Facanali et al. 2015).
Belini (2015) evaluated the genetic diversity through a microsatellite marker
(SSR) and the chemical composition of the essential oils of the native populations
of Baccharis dracunculifolia DC., which occurs in regions with an altitudinal gradi-
ent in Brazil. The populations were collected in the cities of Campos do Jordão
(population J; altitude, 1620 m), Ubatuba (population U; altitude, 2 m), and
Campinas (population C; altitude, 680 m). The population Campos do Jordão
(greatest altitude) presented greater genetic diversity when compared to the other
populations. All populations presented trans-nerolidol as the major substance in
essential oil, ranging from 21.6% to 40.8%. Despite this, the population Campos do
Jordão diverged from the others as to the relative proportions of the substances in the
essential oil. The results suggest the influence of the altitudinal gradient on the
genetic and chemical diversity.
Plants are autotrophic organisms that remove CO2 from the atmosphere and water
and mineral nutrients from the soil. The mineral nutrients are absorbed by plants
mainly by the root system in the inorganic ion form. The acquisition of nutrients by
plants may result from the contribution of fungi (mycorrhizal) and nitrogen-fixing
bacteria. The absorbed ions are transported to the various parts of the plant, where
they are assimilated and used in important biological functions. Mineral nutrition is
the study of how plants absorb, transport, assimilate, and utilize the ions, called
essential elements, without which the plants may not complete their life cycle.
These essential mineral elements are usually classified as macro- or micronutrients,
according to their relative concentration in the tissue or according to the concentra-
tion required for proper growth of the plant. In general, the concentrations of the
macronutrients (N, P, K, Si, Ca, Mg, and S) are higher than those of the micronutri-
ents (Fe, Cu, Zn, Mn, Mo, B, Cl, and Ni) (Marschner 2012).
Inadequate supply of an essential element (excess or deficiency) results in nutri-
tional disorder. These disorders are related to the physiological actions of the ele-
ment in the normal functioning of the plant. Thus, mineral nutrition influences the
primary and specialized metabolism (Marschner 2012).
Related to primary metabolism, Ye et al. (2014) reports that mineral nutrition
plays a key role in photosynthesis since plants require macro- and micronutrients at
some stage of the photosynthetic process (Marschner 2012; Maathuis 2009). Related
to specialized metabolism, several studies have shown that macro- and micronutri-
ents play an influence on the production and profile of volatile substances (Farzadfar
et al. 2017; Pal et al. 2016).
30 C. S. F. Boaro et al.
Our studies, conducted with different plant species, have revealed the influence
of macronutrients on plant growth and development, on gas exchange, and, as a
consequence, on the yield and chemical composition of essential oils produced by
them. Some of these studies are highlighted below.
Bueno (2004) evaluated the development, yield, and chemical composition of
essential oils extracted from the leaves of Thymus vulgaris (thyme) grown in nutri-
ent solution containing different levels of phosphorus (15.5, 31.0, and 46.5 mg.L−1).
The physiological indexes, length, and fresh mass of the aerial part, dry matter of
the root, aerial and total part, aerial part/root relation, and absolute and relative
growth rate were evaluated 62, 83, 104, 125, and 146 days after sowing, while the
yield and chemical composition were determined 65, 95, and 125 days after sowing.
The plants cultivated with the highest level of phosphorus in all crops presented, in
general, greater length, fresh and dry mass of the aerial part, dry mass of the root,
total dry mass, and yield of essential oil. The essential oil of thyme presented 29
substances, and thymol, which has great economic importance, was the main sub-
stance in plants grown with 31.0 mg.L−1 of phosphorus. Plants cultivated in
46.5 mg.L−1 of this nutrient presented higher carvacrol content, showing that the
yield and quality of essential oil of thyme were influenced by an abiotic factor,
represented in this study by phosphorus.
Menthol is the most abundant component of essential oil of adult mint leaves.
The quality and commercial value of essential oils are determined by the balance
between the quantities of their constituents. In general, in mint, quality oils contain
higher amounts of menthol, intermediate amounts of menthone, and low levels of
pulegone and menthofuran. In conditions of abiotic stress, including light, tempera-
ture, and relative humidity, plants of Mentha x piperita L. exhibit accumulation of
pulegone and menthofuran (Brun et al. 1991; Voirin et al. 1990; Mahmoud and
Croteau 2002). The metabolites and intermediate reactions are of great importance
because they are the main determinants of the final production of menthol and its
by-products (Bertea et al. 2001).
The evaluation of the plant development and yield and composition of essential
oil of Mentha x piperita L. leaves grown in nutrient solution with nitrogen levels
(abiotic factor that influences the essential oil) equal to 210 mg.L−1 (complete dose
of N), 263 mg.L−1 (complete dose plus 25% of N), and 315 mg.L−1 (complete dose
plus 50% of N) showed that the plants cultivated with lower nitrogen level
(210/105 mg.L−1) presented higher leaf area and total dry mass, which resulted in
higher photosynthetic efficiency and better yield and quality of essential oil, which
presented higher amount of menthol (Leal 2001).
Mentha x piperita was also grown in nutrient solution containing magnesium
levels equal to 48.6 mg.L−1 (complete dose), 24.3 mg.L−1 (reduction of 50%),
12.1 mg.L−1 (reduction of 75%), and 2.4 mg.L−1 (reduction of 95%). Plant develop-
ment and the essential oil yield were evaluated 21, 49, 63, 77, and 92 days after
transplanting the seedlings to the nutrient solution. The gas exchanges were per-
formed 9, 34, 91, and 109 days after transplanting the seedlings. Plants cultivated
with a reduction of 50% in magnesium compared to the full-level treatment, equal
to 24.3 mg.L−1, presented satisfactory results. Reduction of magnesium to lower
2 Factors Influencing the Production and Chemical Composition of Essential Oils… 31
levels (12.1 and 2.4 mg.L−1), mainly from 77 days after transplantation, impaired
development, gas exchange, and essential oil yield. It should be noted that 2.4 mg.
L−1 of magnesium caused an increase in the essential oil yield before 63 days after
transplantation, which may have occurred due to the low concentration of the min-
eral and consequent stress. In addition to the magnesium level, the harvesting period
also influenced the essential oil yield (Vasques 2007).
David et al. (2007) evaluated the development and the yield of essential oil of
Mentha x piperita L., grown in complete nutrient solution No. 2 of Hoagland and
Arnon (1950) and in the same solution with decrease and increase of 50% of phos-
phorus. The results showed that the variations in phosphorus levels interfered with
the development of Mentha x piperita L. Although the plants showed behavioral
variation for many of the evaluated variables, when submitted to the different levels
of phosphorus, they adapted to this condition, which may be confirmed by the rela-
tive growth rate, which reflects growth. However, those cultivated with the lowest
level of phosphorus, indicative of stress due to the low concentration of this nutrient,
presented higher essential oil yield 60 days after planting, a condition that once
again may be indicative of stress due to low phosphorus concentration. The sub-
stances identified in essential oils in descending order of their contents were men-
thofuran, menthol, menthol acetate, menthone, 1,8-cineole, pulegone, limonene,
β-pinene, isomenthol, α-pinene, and myrcene. The highest contents of menthone
and menthofuran were obtained in cultivated plants at phosphorus levels of 23 and
46.5 mg.L−1, respectively. The highest yield and content of menthol, menthyl ace-
tate, and pulegone were obtained in plants grown at the lowest phosphorus level,
equal to 7.5 mg.L−1 until the first harvest, 60 days after planting (David 2004, 2007;
David, Boaro, and Marques 2006). Phosphorus as an abiotic factor interfered with
the essential oil in mint plants.
Plant development, yield, and chemical composition of Mentha x piperita essen-
tial oil grown in a nutrient solution with N/P/K/Mg variation in different treatments
(50% of N, P, K, and 25% of Mg, containing 94.0/15.5/107.5/12.15 mg.L−1; 50% of
N, P, K, and Mg, containing 94.0/15.5/107.5/24.3 mg.L−1; 65% of N, 50% of P, 25%
of K, and 100% of Mg, containing 124.0/15.5/53.6/48.6 mg.L−1; and with complete
solution containing 189.0/31.0/214.5/48.6 mg.L−1 of N/P/K/Mg) were evaluated 20,
35, 50, 65, and 85 days after transplanting (DAT) the seedlings for nutrient solution.
Higher net assimilation rate (NAR) was verified in plants grown in the complete
nutrient solution up to 35 DAT and lower specific leaf area (SLA) at the beginning
of the cycle. The relative growth rate, in general, was the same in the plants grown
with the different treatments. The highest essential oil yield was obtained in the
plants grown in 65% of N, 50% of P, 25% of K, and 100% of Mg. The major sub-
stances identified in the essential oil were menthol, menthone, menthofuran, neom-
enthol, and menthyl acetate at 69 DAT, not differing among treatments, being,
therefore, the best time for extracting essential oil. The results allow us to conclude
that plants grown with 65% of N, 50% of P, 25% of K, and 100% of Mg showed a
trend of higher mass production, essential oil yield, and menthol content, indicating
that the cultivation of Mentha x piperita L. with these nutrient levels and harvest
season was adequate (David 2007). This study demonstrated that the influence of
32 C. S. F. Boaro et al.
abiotic factors when considering mineral nutrition should take into account the
relationship between nutrients for the fertilization of the species in the production
and chemical composition of the essential oil.
Valmorbida et al. (2006) evaluated the influence of potassium level on the yield
and chemical composition of essential oils extracted from the leaves of Mentha x
piperita L. The plants were cultivated until the 21st day in a nutrient solution of
Hoagland and Arnon (1950) diluted in 50%, passing the complete solution after this
date. The treatments were as follows: T1, complete nutrient solution; T2, nutrient
solution with reduction of 50%; and T3, nutrient solution with reduction of 75%,
harvested at 60, 105, and 120 days after transplantation. Changes in potassium lev-
els and harvesting times did not influence the yield and menthone content, while the
relative percentage of menthol was influenced by potassium levels and harvest
times, with lower content at 120 DAT at the concentration of 58.50/117.00 mg.L−1
(T2, nutrient solution with reduction of 50%).
According to De Fazio (2007, 2011), calcium has been extensively studied, and
the metabolism of this macronutrient needs to be better evaluated since this element
acts as a specialized messenger in routes of signal transduction in plant cells, and,
due to variations in its cellular concentration, acts through modulating proteins and
its target molecules, regulating several cellular processes, from the control of ionic
transport to gene expression.
In this context, there are doubts about the effect of calcium on plant develop-
ment, gas exchange, and the route of production of essential oils, especially yield
and chemical composition.
De Fazio (2007) evaluated the development, yield, and chemical composition of
the essential oil of Mentha x piperita L. grown in a nutrient solution No. 2 of
Hoagland and Arnon (1950) containing 160 mg.L−1 of calcium and in the same
solution with its reduction to 50%, 80 mg.L−1 and 90%, 16 mg.L−1 and subjected to
leaf spraying with 100, 200, and 400 mg.L−1 of ethephon, where they remained until
the harvesting dates, which were performed at 46, 76, 106, and 136 DAT of seed-
lings to the nutrient solution. The length of the aerial part; leaf area; dry matter of
leaf blades, petiole, stems and roots; leaf area ratio (LAR); specific leaf area (SLA);
absolute growth rate (AGR); net assimilation rate (NAR) and relative growth rate
(RGR) and essential oil yield were influenced by the decrease of the calcium level
and by ethephon using. Plants grown with 160 mg.L−1 of calcium showed higher
essential oil yield. Ethephon used in the dosages of 200 and 400 mg.L−1, associated
with all levels of calcium and, mainly, lower and equal to 16 mg.L−1, decreased all
evaluated variables, impairing the development of mint and the yield of its essential
oil, mainly from 106 DAT. Based on the results obtained, it is suggested that the
influence of the calcium level associated with different doses of ethephon in the
development and production of essential oil of mint plants may have occurred due
to the relationship between the cellular concentration of this ion and ethylene bio-
synthesis, considering the participation of calcium in the activity of 1-aminocyclop
ropane- 1-carboxylic acid synthase and 1-aminocyclopropane-1-carboxylic acid
enzymes, which play an important role in ethylene biosynthesis (Kende 1993).
However, future studies should be carried out to confirm this hypothesis.
2 Factors Influencing the Production and Chemical Composition of Essential Oils… 33
When considering the possibility of agricultural use of industrial and urban wastes for
the cultivation of plant species, the contents of heavy metals should be evaluated.
In addition, in Brazil, the application of biosolids in agricultural areas depends on
the prior consent of the governmental body responsible for local environmental
control – Companhia de Tecnologia de Saneamento Ambiental (CETESB) is the
agency of the state of São Paulo responsible for the standards and licensing of use.
Considering only the development of peppermint in the presence of biosolids,
but not discussing the possibility of producing aromatic species in this substrate,
Scavroni et al. (2005) evaluated the effects of biosolid concentrations (0, 28, 56, and
112 ton.ha−1) from the Barueri Sewage Treatment Plant, state of São Paulo, on the
yield and chemical composition of essential oils extracted from Mentha x piperita
leaves at different stages of development. The treatments influenced discreetly the
essential oil yield, increasing when the plants were cultivated with 28 ton.ha−1, a
condition that did not result in better quality, and the presence of biosolids favored
the formation of menthofuran. Menthyl acetate was the major substance in all treat-
ments and menthol was the second largest substance observed (42.3%) at 90 DAT in
plants cultivated without biosolids. With the development of the plants, there was a
decrease of menthol and menthone. It is recommended to harvest mint at 90 DAP, at
which point the menthol level was higher. Although the cultivation with 28 ton.ha−1
is within the limits allowed by the legislation, this condition, which increased the oil
yield, did not improve its quality. Thus, the biosolids of the Barueri Plant are not
recommended for the cultivation of mint.
Water stress in plant species is an abiotic factor which influences the physiological
processes, changes the metabolic homeostasis, and imposes adjustment of the meta-
bolic routes (Shulaev et al. 2008; Ciarmiello et al. 2011). Water limitation has a
negative effect on plant growth and development; however, moderate water defi-
ciencies in some medicinal, aromatic, or spice species may result in the accumula-
tion of bioactive substances due to the adverse condition. Under these conditions,
the plant continues to perform photosynthesis, but its growth decreases and the
excess photoassimilates produced are redirected to specialized metabolism
(Marchese and Figueira 2005). Moreover, the detrimental effects of the stress may
be compensated by the plants through several mechanisms that operate at different
time scales, depending on the nature of the stress and the physiological processes
that are affected (Lambers et al. 1998). In this way, evaluating and understanding
the metabolic responses of medicinal species under the influence of water suppres-
sion may provide important information on the management of these plants under
adverse conditions and enable the higher production of target substances.
Búfalo et al. (2016) evaluated the influence of osmotic stress by applying two
concentrations of polyethylene glycol (50 g.L−1 and 100 g.L−1 of PEG) over a short
period of time in the anatomy and leaf ultrastructure in the physiological pattern of
2 Factors Influencing the Production and Chemical Composition of Essential Oils… 35
M. x piperita and in the essential oil profile. The results indicated that the responses
to osmotic stress were dose-dependent, since the plants submitted to 50 g.L−1 of
PEG maintained the structural aspects and metabolic functions similar to the control
treatment plants (0 g.L−1 of PEG), without changes in structural characteristics and
gas exchange. The increased activity of antioxidant enzymes reduced the presence
of free radicals and protected the membranes, including those of chloroplasts and
mitochondria. The osmotic stress caused by 100 g.L−1 of PEG inhibited the gas
exchange, reduced the yield of essential oil, and caused a change in its composition,
with a decrease of menthone and increase of menthofuran.
Bolina (2015) evaluated the physiological, chemical, and biochemical responses
of micropropagated plants of Varronia curassavica Jacq. (erva baleeira) in function
of the suppression of irrigation. The treatments were represented by the control
(daily irrigated) and by three levels of water deficiency, all expressed by water
potential in the leaf xylem (Ψw): T1, control (Ψw~ −0.3 MPa), T2 (Ψw~ −1.0 MPa),
T3 (Ψw~ −1.7 MPa), and T4 (Ψw~ −2.5 MPa). The results showed a reduction in
plant height, leaf area and yield of fresh leaf, and stem and total mass, due to water
suppression. The gaseous exchanges were similar between treatments. The relative
water content decreased linearly in relation to the different levels of water potential,
presenting a reduction of about 80%. The lower water potential (−2.5 MPa) pro-
vided a higher yield of essential oil (0.18%). The potential of −1.7 MPa led to the
limit of activity of the enzyme superoxide dismutase and the greater accumulation
of proline. The suppression of irrigation led to an increase in the essential oil of V.
curassavica. There was no quantitative difference in essential oils between treat-
ments, except for γ-(E)-bisabolene. The relative proportions of the active principles
(E)-caryophyllene (25.2%) and α-humulene (4.4%) did not differ between
treatments.
Allelopathy may be defined as the science that studies the processes involving spe-
cialized metabolites produced by living organisms that have influence in inhibiting
or stimulating the growth and development. Allelopathic substances synthesized by
routes of specialized metabolism and released to the environment are called allelo-
chemicals (Rice 1984) and may influence the development and establishment of
agricultural crops and plant communities (Torres et al. 1996; Reigosa et al. 2013).
Considering the concentration and structure of the molecules, the use of allelo-
pathic extracts in the cultivation of medicinal plants may provide different and
advantageous responses in their development and in the yield and composition of
the essential oil produced. The interaction of the molecules with the routes of the
specialized metabolism may explain the effects of allelochemicals in the develop-
ment of different species and elucidate the chemical-ecological mechanisms of
the plant-plant interaction. On the other hand, nutritional conditions combined
with allelopathic effects may shape the chemical profile of the substances of the
36 C. S. F. Boaro et al.
The seasonality and the edaphoclimatic factors (light, water, temperature, and soil)
that characterize it may influence physiological and biochemical processes and/or
metabolic routes, interfering with the synthesis of important specialized compounds,
such as terpenes (Shao et al. 2001; Gobbo-Neto and Lopes 2007).
The period of the year in which a substance of specialized metabolism is identi-
fied is of great importance since it depends on the quantity and nature of these
substances, not constant during the year. There are many reports of variations of
classes of specialized metabolites, such as essential oils, due to seasonality and
edaphoclimatic factors.
Isobe (2012) evaluated the influence of seasonality, summer and winter, on the
yield and chemical composition of essential oils of three native populations of
Lychnophora pinaster Mart., collected in the cities of Carrancas (population Areia
Branca), Lavras (population Poço Bonito), and Itumirim (population Serra do Sofá),
state of Minas Gerais. The two evaluated periods did not influence the average yield
of essential oils within the three populations. Regardless of the season, the major
substances in the leaves of essential oils of the three populations of L. pinaster were
methyl trans-cinnamate and trans-caryophyllene, which presented different relative
proportions between the populations. During summer, essential oils presented a
higher relative proportion of methyl trans-cinnamate, and during winter the highest
relative proportion was trans-caryophyllene for all populations.
Silva (2013) evaluated the influence of seasonality (summer, autumn, winter, and
spring) on the yield and chemical composition of essential oils of the leaves from
three native populations of Lychnophora pinaster – two collected in the city of
Carrancas (MG), named Serra Branca and Serra do Salto, and one in the city of
Ingaí (MG), named Serra da Arnica. The average yield of essential oil of the popu-
lations Serra Branca and Serra do Salto was not influenced by seasonality, and the
population of Serra do Salto showed a higher average yield (0.43% vs. 0.61%) in all
seasons during the year. The average yield of essential oil of the population Serra da
2 Factors Influencing the Production and Chemical Composition of Essential Oils… 37
Arnica was influenced by seasonality, and the highest yield occurred in winter
(0.79%). The major substances of essential oil in the three populations were methyl
trans-cinnamate and trans-caryophyllene, and their relative proportions varied with
seasonality. In Serra Branca, trans-caryophyllene presented the highest relative
proportion during spring, in Serra da Arnica during autumn, and in Serra do Salto
during summer (14.2%), which did not differ from autumn (12.3%).
It should be emphasized that the sites where the above-registered populations
were evaluated have different edaphoclimatic conditions, which, although not con-
sidered in the studies, interact with seasonality and interfere with essential oils.
2.5 B
iological Activity of the Essential Oil and Isolated
Substances
The species Mentha x piperita L.; Ocimum selloi Benth, reclassified as Ocimum
carnosum (Spreng.) Link and Otto ex Benth; Ocimum basilicum L.; Origanum vul-
gare L.; Thymus vulgaris L.; Lychnophora ericoides Mart.; Lychnophora pinaster
Mart;, Baccharis dracunculifolia DC; and Varronia curassavica Jacq (synonym:
Cordia verbenacea DC) were also evaluated by other authors, who evaluated the
biological activities of their oils or chemical substances, and were isolated and
observed their antibacterial, analgesic, antispasmodic, antiviral, anticancer, larvici-
dal, antifungal, and acaricidal actions, as presented in Table 2.1.
Besides these, other studies conducted may be identified in the specialized
literature.
The biological activity of essential oils of several species and isolated substances
has been described in the literature. The essential oil from Mentha x villosa Hudson
and the substance rotundifolone showed larvicidal activity (Lima et al. 2014).
Essential oil of Croton heliotropiifolius, Kunth revealed antibacterial activity
(Araújo et al. 2017; Alencar Filho et al. 2017) and essential oil of Schinus molle an
insecticidal activity (López et al. 2014).
Lima et al. (2014) evaluated the larvicidal activity of essential oil of Mentha x
villosa and its major substance, rotundifolone (70%), against Aedes aegypti larvae.
The essential oil had excellent larvicidal activity (LC50 = 45.0 ppm), while rotundi-
folone presented moderate larvicidal activity (LC50 = 62.5 ppm).
Essential oils of 11 species of Piper collected in Mata Atlântica, state of São
Paulo, were evaluated for antimicrobial activity. Essential oils of most species
showed up to 30% inhibitory activity against pathogenic in vitro bacteria (E. coli,
S. epidermidis, S. aureus, and C. xerosis) in relation to commercial antibiotics. In
these essential oils, the proportion of substances bicyclogermacrene and γ-muurolene
were positively associated with inhibition of E. coli, whereas the proportions of
limonene and cis-β-ocimene inhibited Staphylococcus aureus, and those of ger-
macrene D and trans-caryophyllene were associated with inhibitory activity against
all evaluated pathogens. The results demonstrated a chemical diversity of Piper’s
38 C. S. F. Boaro et al.
essential oils and their potential as new antibacterial agents for several industrial
applications (Perigo et al. 2016).
Essential oils extracted from the leaves of Duguetia gardneriana and Duguetia
moricandiana were tested for antimicrobial activity against 11 pathogenic microor-
ganisms using the standard gel diffusion method. The major substances identified in
this essential oil of D. gardneriana leaves were germacrene D (28.1%), viridiflorene
(24.0%), β-pinene (12.6%), α-pinene (9.1%), and β-caryophyllene (5.6%) and of
D. moricandiana leaves were germacrene D (44.3%), α-pinene (13.0%), viridiflo-
rene (9.3%), β-pinene (9.2%), and β-caryophyllene (6.8%). Essential oil of D. gard-
neriana showed activity against Staphylococcus aureus and Candida guilliermondii,
and essential oil of D. moricandiana showed higher activity against Staphylococcus
aureus and Candida albicans (Almeida et al. 2010).
2.6 Conclusions
The studies show that variations occur in the chemical compositions of essential oils
of plant species cultivated or grown under conditions whose abiotic factors vary. In
addition, the studies show a genetic and chemical diversity of essential oils among
populations of native species of natural occurrence in different regions of Brazil.
Acknowledgments The authors thank Fundação de Apoio à Pesquisa do Estado de São Paulo
(FAPESP), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), and
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for financial support for
research and scholarship.
References
Abdelwahab SI, Faridah QZ, Mariod AA et al (2010) Chemical composition, antioxidant and
antibacterial properties of the essential oils of Etlingera elatior and Cinnamomum pubescens
Kochummen. J Sci Food Agric 90:2682–2688
Adams RP (2017) Identification of essential oil components by gas chromatography, Texensis
Publishing Gruver, TX USA.
Ahmad A, Khan A, Akhtar F et al (2011) Fungicidal activity of thymol and carvacrol by disrupting
ergosterol biosynthesis and membrane integrity against Candida. Eur J Clin Microbiol Infect
Dis 30:41–50
Akisue MK, Oliveira F, Moraes MS et al (1983) Caracterização farmacognóstica da droga e da tin-
tura de Cordia verbenacea AL. DC. Boraginaceae. Revista de Ciências Farmacêuticas 5:69–82
Alencar Filho JMT, Araújo LC, Oliveira AP et al (2017) Chemical composition and antibacterial
activity of essential oil from leaves of Croton heliotropiifolius in different seasons of the year.
Rev Bras Farmacogn 27:440–444
Allendorf FW, Hohenlohe PA, Luikart G (2010) Genomics and the future of conservation genetics.
Nat Rev Genet 11:697–709
Almeida JRGS, Facanali R, Vieira MAR (2010) Composition and antimicrobial activity of the leaf
essential oils of Duguetia gardneriana Mart. and Duguetia moricandiana Mart. (Annonaceae).
J Essent Oil Res 22:275–278
2 Factors Influencing the Production and Chemical Composition of Essential Oils… 39
Al-Shehbaz IA (1991) The genera of Boraginaceae in the southeastern United States. J Arnold
Arboretum Suppl 1:1–169
Amdouni T, Ben Abdallah S, Msilini N et al (2016) Effect of salt stress on the antimicrobial activity
of Ruta chalepensis essential oils. Acta Physiol Plant 38:147
Antunes MDC, Cavaco AM (2010) The use of essential oils for postharvest decay control. A review.
Flavour Fragr J 25:351–366
Antunes T, Sevinate-Pinto I, Barroso JG et al (2004) Micromorphology of trichomes and composi-
tion of essential oil of Teucrium capitatum. Flavour Fragr J 19:336–340
Araújo FM, Dantas MCSM, Silva LS et al (2017) Antibacterial activity and chemical composition
of the essential oil of Croton heliotropiifolius Kunth from Amargosa, Bahia, Brazil. Ind Crop
Prod 105:203–206
Aslam F, Khaliq A, Matloob A et al (2017) Allelopathy in agro-ecosystems: a critical review of
wheat allelopathy-concepts and implications. Chemoecology 27:1–24
Aznar A, Fernandez PS, Periago PM et al (2015) Antimicrobial activity of nisin, thymol, carvacrol
and cymene against growth of Candida lusitaniae. Food Sci Technol Int Ibaraki 21:72–79
Baldin ELL, Dal Pogetto MHFA, Pavarini DP et al (2010) Composição química e atividade
acaricida do óleo essencial de Lychnophora ericoides Mart. sobre Tetranychus urticae Koch
(Acari: Tetranychidae). Boletín de Sanidad Vegetal, Plagas 36:125–132
Belini CMB (2015) Baccharis dracunculifolia DC. (Asteraceae): composição do óleo essencial,
diversidade e parâmetros genéticos. Thesis, Universidade Estadual Paulista “Júlio de Mesquita
Filho” Faculdade de Ciências Agronômicas
Bertea CM, Schalk M, Karp F et al (2001) Demonstration that menthofuran synthase of mint
(Mentha) is a cytochrome P450 monooxygenase: cloning, functional expression, and charac-
terization of the responsible gene. Arch Biochem Biophys 390:279–286
Bird A (2007) Perceptions of epigenetics. Nature (London) 447:396–398
Blank AF, Carvalho Filho JLS, Neto S et al (2004) Caracterização morfológica e agronômica de
acessos de manjericão e alfavaca. Hortic Bras 22:113–116
Bolina C (2015) Metabolismo, Desenvolvimento e composição química de Varronia curassavica
Jacq. em função da supressão da irrigação. Thesis, Universidade Estadual Paulista “Júlio de
Mesquita Filho” Faculdade de Ciências Agronômicas
Bowers WS, Nishida R (1980) Juvocimenes: potent juvenile hormones mimics from sweet basil.
Science 209:1030–1332
Bozin B, Mimica-Dukic N, Simin N, Anackov G (2006) Characterization of the volatile composi-
tion of essential oils of some Lamiaceae spices and the antimicrobial and antioxidant activities
of the entire oils. J Agric Food Chem 54:1822–1828
Braga PC, Alfieri M, Culici M, Dal Sasso M (2007) Inhibitory activity of thymol against the
formation and viability of Candida albicans hyphae. Mycoses 50:502–506
Brun N, Colson M, Perrin A, Voirin B (1991) Chemical and morphological studies ofThe
effects of ageing on monoterpene composition in Mentha x piperita leaves. Can J Bot
69:2271–2278
Buchanan BB, Gruissem W, Jones RL (2015) Biochemistry & molecular biology of plants. Wiley,
Chichester
Bueno MAS (2004) Níveis de fósforo no desenvolvimento e produção de óleo essencial de Thymus
vulgaris L. cultivado em solução nutritiva. Dissertação, Universidade Estadual Paulista Júlio
de Mesquita Filho
Búfalo J (2015) Mentha X Piperita, Ocimum Basilicum e Salvia Deserta, (Lamiaceae):
Abordagens Fisiológicas E Fitoquímicas Mentha X Piperita, Ocimum Basilicum E Salvia
Deserta, (Lamiaceae): Abordagens fisiológicas e fitoquímicas. thesis Universidade Estadual
Paulista Júlio de Mesquita Filho
Búfalo J, Rodrigues TM, de Almeida LFR et al (2016) PEG-induced osmotic stress in Mentha
x piperita: structural features and metabolic responses. Plant Physiol Biochem 105:174–184
Canceli RR, Evaldt ACP, Bauermann SG (2007) Contribuição da morfologia polínica da famí-
lia Asteraceae Martinov. no Rio Grande do Sul – Parte I. Pesquisas Botânica São Leopoldo
58:347–374
40 C. S. F. Boaro et al.
Carboni TR (2013) Análise de crescimento, trocas gasosas, potencial antioxidante e óleo essencial
de Origanum vulgare L. ssp. vulgare. 2013. Dissertation, Universidade Estadual Paulista Júlio
de Mesquita Filho
Carneiro MAA, Fernandes GW (1996) As relações conflituosas entre plantas e insetos, Herbivoria.
Ciência Hoje 20:32–35
Carreto CFP (2010) Atividade antimicrobiana de Mentha piperita L. sobre leveduras do gênero
Candida. Dissertation, Faculdade de Odontologia de São José dos Campos
Carvalho Júnior PM, Rojasa LB, Velasco J et al (2004) Chemical composition and antimicrobial
activity of the essentialoil of Cordia verbenacea D.C. J Ethnopharmacol 95:297–301
Cassel E, Frizzo CD, Vanderlinde R, Atti-Serafini L, Lorenzo D, Dellacassa E (2000) E. Extraction
of Baccharis oil by supercritical CO2. Montevideo, Uruguai. Ind Eng Chem Res 39:4803–4805
Cattelan MG (2015) Atividade antibacteriana de óleo essencial de orégano (Origanum vulgare):
ações in vitro e in situ para preservação de alimento. Thesis, Universidade Estadual Paulista
“Júlio de Mesquita Filho”
Chacón IV, Riley-Saldaña CA, González-Esquinca AR (2013) Secondary metabolites during early
development in plants. Phytochemistry Reviews 12(1):47–64
Chezem WR, Clay NK (2016) Regulation of plant secondary metabolism and associated special-
ized cell development by MYBs and bHLHs. Phytochemistry 131:26–43
Cho RJ, Mindrinos M, Richards DR et al (1999) Genome-wide mapping with biallelic markers in
Arabidopsis thaliana. Nat Genet 23:203–207
Ciarmiello LF, Woodrow P, Fuggi A et al (2011) Chapter 13: Abiotic stress in plants – mechanisms
and adaptations. In: Plant Genes for abiotic stress. InTech, Rijeka
Coile NC, Jones SB (1981) Lychnophora (Compositae: Vernonieae), a genus endemic to the
Brazilian Planalto. Briffonia 33:528–542
Cola M et al (2003) Óleo essencial de Ocimum selloi Benth.: atividade antiulcerogênica.
Documentos IAC 74:120
David EFS (2004) Níveis de fósforo no desenvolvimento e produção de óleo essencial de Mentha
piperita L. cultivada em solução nutritiva. Dissertation, Universidade Estadual Paulista Júlio
de Mesquita Filho
David EFS (2007) Desenvolvimento, trocas gasosas, rendimento e composição de óleo essencial
de Mentha Piperita L cultivada em solução nutritiva com variação dos níveis de N, P, K E Mg.
2007. Thesis, Universidade Estadual Paulista Júlio de Mesquita Filho
David EF, Boaro CSF, Marques MOM (2006) Rendimento e composição do óleo essencial de
Mentha piperita L., cultivada em solução nutritiva com diferentes níveis de fósforo. Rev Bras
Plantas Med 8:183–188
David EFS, Pirozzi DCZ, Braga JF et al (2007) Desenvolvimento do manjericão (Ocimum basili-
cum L.) cultivado em solução nutritiva com diferentes níveis de magnésio. Revista Brasileira
de Plantas Medicinais 9:15–22
De Fazio JL (2007) Influência de cálcio e de ethephon no desenvolvimento e produção de óleo
essencial de menta (Mentha piperita L.), cultivada em solução nutritiva 2007. Dissertation,
Universidade Estadual Paulista Júlio de Mesquita Filho
De Fazio JL (2011) Mentha piperita cultivada com variação de cálcio. Trocas gasosas e óleo essen-
cial. 2011. Thesis, Universidade Estadual Paulista Júlio de Mesquita Filho
De Queiroz ACM, Rabello AM, Braga DL et al (2017) Cerrado vegetation types determine how
land use impacts ant biodiversity. Biodivers Conserv 26:1–18
De Vries J, Evers JB, Poelman EH (2017) Dynamic plant-plant-herbivore interactions govern plant
growth–defence integration. Trends Plant Sci 22:329–337
Degenhardt J, Hiltpoldb I, Köllner TG et al (2009) Restoring a maize root signal that attracts
insectkilling nematodes to control a major pest. Proc Natl Acad Sci U S A 106:13213–13218
Dewick PM (2009) Medicinal natural products: a biosynthetic approach, 3rd edn. Wiley, Chichester
Dickison WC (2000) A Intergrative plant anatomy. Academic Press, London
Dorman HJD, Deans SG (2000) Antimicrobial agents from plants: antibacterial activity of plant
volatile oils. J Appl Microbiol 88:308–316
2 Factors Influencing the Production and Chemical Composition of Essential Oils… 41
Duarte AR, Naves RR, Santos SC et al (2010) Genetic and environmental influence on essential oil
composition of Eugenia dysenterica. J Braz Chem Soc 21:1459–1467
Edris AE, Farrag ES (2003) Antifungal activity of peppermint and sweet basil essential oils and
their major aroma constituents on some plant pathogenic fungi from the vapor phase. Nahrung
47:117–121
Elgayyar M, Draughon FA, Golden DA, Mount JR (2001) Antimicrobial activity of essen-
tial oils from plants selected pathogenic and saprophytic microorganisms. J Food Prot
64:1019–1024
Facanali R, Colombo CA, Teixeira JPF et al (2015) Genetic and chemical diversity of native popu-
lations of Ocimum selloi Benth. Ind Crop Prod 76:249–257
Farzadfar S, Zarinkamar F, Hojati M (2017) Magnesium and manganese affect photosynthesis,
essential oil composition and phenolic compounds of Tanacetum parthenium. Plant Physiol
Biochem 112:207–217
Fernandes ES, Passos GF, Medeiros R et al (2007) Anti-inflammatory effects of compounds alpha-
humulene and (−)-trans-caryophyllene isolated from the essential oil of Cordia verbenacea.
Eur J Pharmacol 569:228–236
Fernandes MG, Gomes RA, Brito-Filho SG (2014) Characterization and anti-staphylococcal activ-
ity of the essential oil from Turnera subulata Sm. Revista Brasileira de Plantas Medicinais
16:534–538
Ferraz RPC, Cardoso GMB, Silva TB et al (2013) Antitumour properties of the leaf essential oil of
Xylopia frutescens Aubl. (Annonaceae). Food Chem 141:542–547
Ferreira ME (2001) Técnicas e estratégias para a caracterização molecular e uso de recursos gené-
ticos. In: Garray I, Dias BFS (eds) Conservação da Biodiversidade em ecossistemas tropicais:
avanços conceituais e revisão de novas metodologias de avaliação e monitoramento. Petrópolis,
Vozes, p 2001
Figueiredo C, Barroso J, Pedro L et al (2007) Factors affecting secondary metabolite production in
plants: volatile components and essential oils. Flavour Fragr J 22:206–213
Figueiredo A, Barroso JG, Pedro LG et al (2008) Portuguese Thymbra and Thymus species
volatiles: chemical composition and biological activities. Curr Pharm Des 14:3120–3140
Flora do Brasil (2020) Flora do Brasil. http://floradobrasil.jbrj.gov.br/reflora/floradobrasil/
FB110219. Accessed 31 July 2017
Frankham R, Ballou JD, Briscoe DA, Mclnnes KH (2008) Fundamentos de Genética da
Conservação. Sociedade Brasileira de Genética, Ribeirão Preto.
Funk VA, Bayer RJ, Keeley S et al (2005) Everywhere but Antarctica: using a super tree to under-
stand the diversity of the Compositae. Biologiske Skrifter 55:343–374
Galindez G, Biganzoli F, Ortega-Baes P et al (2009) Fire responses of three co-occurring Asteraceae
shrubs in a temperature savanna in South America. Plant Ecol 202:149–158
Giordani R, Regli P, Kaloustian J et al (2004) Antifungal effect of various essential oils against
Candida albicans. Potentiation of antifungal action of amphotericin B by essential oil from
Thymus vulgaris. Phytother Res 18:990–995
Gobbo-Neto L, Lopes NP (2007) Plantas Medicinais: Fatores de Influência no Conteúdo de
Metabólitos Secundários. Química Nova 30:374–381
Goggin FL (2007) Plant-aphid interactions: molecular and ecological perspectives. Curr Opin
Plant Biol 10:399–408
Gouinguené SP, Turlings TCJ (2002) The effects of abiotic factors on induced volatile emissions
in corn plants. Plant Physiol 129:1296–1307
Goutham MP (1980) Activity of some essential oils against dermatophytes. In: Lorenzi H, Matos
FJA (eds) Plantas medicinais do Brasil: nativas e exóticas cultivadas. Nova Odessa: Instituto
Plantarum, 2002
Guenther E (1948) The essential oils: history-origin in plants production-analysis. Krieger
Publishing, New York
Gupta R (1991) Agrotechnology of medicinal plants. In: Wijessekera ROB (ed) The medicinal
plant industry. CRC Press, Boca Raton
42 C. S. F. Boaro et al.
Gupta AK, Mishra R, Singh AK et al (2016) Genetic variability and correlations of essential oil
yield with agro-economic traits in Mentha species and identification of promising cultivars. Ind
Crop Prod 95:726–732
Haber LL (2008) Caracterização da diversidade genética , via marcador microssatélite, e constitu-
intes do óleo essencial de lychnophora pinaster mart. Thesis, Universidade Estadual Paulista
Júlio de Mesquita Filho
Harley R, França F, Santos EP, Santos JS, Pastore JF (2015) Lamiaceae in Lista de Espécies da
Flora do Brasil. Jardim Botânico do Rio de Janeiro. http://floradobrasil.jbrj.gov.br/jabot/flora-
dobrasil/FB142. Accessed: 18 outubro 2016.
Heiden G, Barbieri RL, Wasum RA et al (2007) A família Asteraceae em São Mateus do Sul,
Paraná. Revista Brasileira de Biociências 5:249–251
Hoagland DR, Arnon DI (1950) The water: culture method for growing plants without soil.
Berkeley, California
Hussain AI, Anwar F, Sherazi STH, Przybylski R (2008) Chemical composition, antioxidant and
antimicrobial activities of basil (Ocimum basilicum) essential oils depends on seasonal varia-
tions. Food Chemistry 108(3):986–995
Ietswaart JH (1980) A taxonomic revision of the genus Origanum (Labiatae). Leiden University,
Leiden
Isobe MTC (2012) Lychnophora pinaster: estudo anatômico, propagação e composição química
dos óleos essenciais de populações. Dissertation, Universidade Estadual Paulista Júlio de
Mesquita Filho
Jamieson MA, Burkle LA, Manson JS et al (2017) Global change effects on plant–insect interac-
tions: the role of phytochemistry. Curr Opin Insect Sci 23:70–80
Joly AB (1993) Botânica: introdução à taxonomia vegetal. Companhia Editora Nacional, São Paulo
Kamanula JF, Belmain SR, Hall DR et al (2017) Chemical variation and insecticidal activity of
Lippia javanica (Burm. f.) Spreng essential oil against Sitophilus zeamais Motschulsky. Ind
Crops Prod 110:75–82
Karp A, Kresovich S, Bhat KV et al (1997) Molecular tools in plant genetic resources conserva-
tion a guide in the technologies. IPGRI, Rome
Keita SM, Vincent C, Schmit JP et al (2001) Efficacy of essential oil of Ocimum basilicum L. and
O. gratissimum L. applied as an insecticidal fumigant and powder to control Callosobruchus
maculatus (Fab) (Coleoptera: Bruchidae). J Stored Prod Res 37:339–349
Kende H (1993) Ethylene biosynthesis. Annu Rev Plant Physiol Plant Mol Biol 44:283–307
Kim SH, Lee S, Piccolo SR et al (2012) Menthol induces cell-cycle arrest in PC-3 cells by
down-regulating G2/M genes, including polo-like kinase 1. Biochem Biophys Res Commun
422:436–441
Klaric MS, Kosalec I, Mastelic J et al (2007) Antifungal activity of thyme (Thymus vulgaris L.)
essential oil and thymol against moulds from damp dwellings. Lett Appl Microbiol 44:36–42
Klimánková E, Holadová K, Hajslová J et al (2008) Aroma profiles of five basil (Ocimum basili-
cum L.) cultivars grown under conventional and organic conditions. Food Chem 107:464–472
Kopsell DA, Kopsell DE, Curran-Celentano J (2005) Carotenoid and chlorophyll pigments in
sweet basil grown in the field and greenhouse. Hortscience 40:1230–1233
Kroymann J (2011) Natural diversity and adaptation in plant secondary metabolism. Curr Opin
Plant Biol 14:246–251
Kumar S, Wahab N, Warikoo R (2011) Bioefficacy of Mentha piperita essential oil against dengue
fever mosquito Aedes aegypti L. Asian Pac J Trop Biomed 1:85–88
Lambers H, Chapin FS, Pons TL (1998) Plant physiological ecology. Springer, New York
Langenheim JH (2003) Plant Resins: Chemistry, Evolution, Ecology, and Ethnobotany. Timber
Press, Oregon
Lawrence BM (1995) The isolation of aromatic materials from natural plant products. In: Tuley de
Silva K (ed) A manual on the essential oil industry. Proceedings of the 3rd UNIDO workshop
on essential oil and aroma chemical industries, pp 57–154
Leal FP (2001) Desenvolvimento, produção e composição de óleo essencial da Mentha piperita L.,
cultivada em solução nutritiva com diferentes níveis de nitrogênio. Dissertation, Universidade
Estadual Paulista Júlio de Mesquita Filho
2 Factors Influencing the Production and Chemical Composition of Essential Oils… 43
Li Q, Wang X, Yang Z et al (2009) Menthol induces cell death via the TRPM8 channel in the
human bladder cancer cell line T24. Oncology 77:335–341
Lima TC, Silva TKM, Silva FL et al (2014) Larvicidal activity of Mentha × villosa Hudson essen-
tial oil, rotundifolone and derivatives. Chemosphere 104:37–43
Loewenfeld C, Back F (1980) Guia de las hierbas y especias. Ediciones Omega, Barcelona
López A, Castro S, Andina MJ et al (2014) Insecticidal activity of microencapsulated Schinus
molle essential oil. Ind Crop Prod 53:209–216
Lorenzi H, Matos FJA (2008) Plantas medicinais no Brasil: nativas e exóticas, 2nd edn. Instituto
Plantarum, Nova Odessa
Maathuis FJM (2009) Physiological functions of mineral macronutrients. Curr Opin Plant Biol
12:250–258
Mahmoud SS, Croteau RB (2002) Strategies for transgenic manipulation of monoterpene biosyn-
thesis in plants. Trends Plant Sci 7:366–373
Marchese JÁ, Figueira GM (2005) O uso de tecnologias pré e pós-colheita e boas práticas agríco-
las na produção de plantas medicinais e aromáticas. Revista Brasileira de Plantas Medicinais
7:86–96
Marin M, Koko V, Duletić-Laušević S et al (2006) Glandular trichomes on the leaves of Rosmarinus
officinalis: morphology, stereology and histochemistry. S Afr J Bot 72:378–382
Marotti M, Piccaglia R, Giovanelli E (1996) Differences in essential oil composition of Basil
(Ocimum basilicum L.) Italian cultivars related to morphological characteristics. J Agric Food
Chem 44:3926–3929
Marques MOM, Facanali R, Haber LL et al (2012) Essential oils: history, biosynthesis, and agro-
nomic aspects. In: Medicinal essential oils: chemical, pharmacological and therapeutic aspects.
Nova Science Publishers, New York
Marques MOM, Facanali R, Haber LL et al (2013) Óleos essenciais. In: Haber LL, Clemente
FMVT (eds) Plantas aromáticas e condimentares: uso aplicado na horticultura. Embrapa,
Brasília
Marschner H (2012) Mineral nutrition of higher plants, 3rd edn. Academic Press, San Diego
Martins ER, Casali VWD, Barbosa LCA et al (1997) Essential oil in the taxonomy of Ocimum
selloi Benth. J Braz Chem Soc 8:29–32
Matos-Rocha T, Cavalcanti MGS, Barbosa-Filho JM et al (2013) In vitro evaluation of schistoso-
micidal activity of essential oil of Mentha × villosa and some of its chemical constituents in
adult worms of Schistosoma mansoni. Planta Med 79:1307–1312
Matos-Rocha TJ, Cavalcanti MGS, Veras DL et al (2016) Ultrastructural changes in Schistosoma
mansoni male worms after in vitro incubation with the essential oil of Mentha × villosa Huds.
Revista Do Instituto de Medicina Tropical de Sao Paulo 58:2–7
Matos-Rocha TJ, Cavalcanti MGS, Barbosa-Filho JM et al (2017) Ultrastructural study of mor-
phological changes in Schistosoma mansoni after in vitro exposure to the monoterpene rotun-
difolone. Sociedade Brasileira De Medicina Tropical 50:86–91
Medeiros R, Passos GF, Vitor CE et al (2007) Effect of two active compounds obtained from the
essential oil of Cordia verbenacea on the acute inflammatory responses elicited by LPS in the
rat paw. Br J Pharmacol 151:618–627
Meldau S, Erb M, Baldwin IT (2012) Defence on demand: mechanisms behind optimal defence
patterns. Ann Bot 110:1503–1514
Melo WJ, Marques MOM (2000) Potencial do lodo de esgoto como fonte de nutrientes para as
plantas. In: Bettiol W, Camargo OA (eds) Impacto ambiental do uso agrícola do lodo de esgoto.
Embrapa Meio Ambiente, Jaguariúna, pp 109–141
Milach SCK (1998) Principais tipos de marcadores moleculares e suas características. In: Milach
SCK (ed) Marcadores moleculares em plantas. UFRGS, Porto Alegre
Miller JS, Gottschling M (2007) Generic classification in the Cordiaceae (Boraginales): resurrec-
tion of the genus Varronia P Br. Taxon 56:163–169
Millezi FM, Pereira MO, Batista NN et al (2012) Susceptibility of monospecies and dual-species
biofilms Staphylococcus aureus and Escherichia coli to essential oils. J Food Saf 32:351–359
Millezi FM, Cardoso MG, Alves E et al (2013) Reduction of Aeromonas hydrophila biofilm on
stainless steel surface by essential oils. Braz J Microbiol 44:73–80
44 C. S. F. Boaro et al.
Mockute D, Bernotiene G, Judzentiene A (2001) The essential oil of Origanum vulgare L. ssp.
vulgare growing wild in Vilnius district (Lithuania). Phytochemistry 57:65–69
Mockute D, Bernotiene G, Judzentiene A (2003) The β-ocimene chemotype of essential oils of the
inflorescences and the leaves with stems from Origanum vulgare ssp. vulgare growing wild in
Lithuania. Biochem Syst Ecol 31:269–278
Molt O, Trka A (1983) Parfum Kostmet 64: 488. apud Verdi LG 2005.
Montanari I Jr (2000) Cultivo comercial de erva-baleeira. Revista Agroecologia Hoje 3:14–15
Monteith GR, McAndrew D, Faddy HM et al (2007) Calcium and cancer: targeting Ca2+ trans-
port. Nat Rev Cancer 7:519–530
Morhy L (1973) Metil-chavicol, cis e trans-Anetol no Óleo Essencial de Ocimum selloi Benth. An
Acad Bras Ciênc 45:401–412
Morris JA, Khettry A, Seitz EWM (1979) Antimicrobial activity of aroma chemicals and essential
oils. J Am Oil Chem Soc 56:595–603
Mors WB, Rizzini CT, Pereira NA (2000) Medicinal plants of Brazil. Reference Publications,
Algonac
Morshedloo MR, Craker LE, Salami A et al (2017) Effect of prolonged water stress on essential
oil content, compositions and gene expression patterns of mono- and sesquiterpene synthesis in
two oregano (Origanum vulgare L.) subspecies. Plant Physiol Biochem 111:119–128
Munsi OS (1992) Nitrogen and phosphorus nutrition response in Japanese mint cultivation. Acta
Hortic 306:436–443
Nagegowda DA (2010) Plant volatile terpenoid metabolism: biosynthetic genes, transcriptional
regulation and subcellular compartmentation. FEBS Letters 584(14):2965–2973
Naghibi F, Mosaddegh M, Motamed SM et al (2005) Labiatae Family in folk medicine in Iran:
from Ethnobotany to pharmacology. Iran J Pharm Res 2:63–79
Nakajima J, Loeuille B, Heiden, G et al (2015) Asteraceae in Lista de Espécies da Flora do Brasil.
Jardim Botânico do Rio de Janeiro. http://floradobrasil.jbrj.gov.br/jabot/floradobrasil/FB55
Accessed 03 out. 2017
Nascimento KF, Moreira FMF, Santos JA, Kassuia CAL, Croda JHR, Cardoso CAL, Vieira
MC, Ruiz ALTG, Foglio MA, de Carvalho JE, Formagio ASN (2017) Antioxidant, anti-
inflammatory, antiproliferative and antimycobacterial activities of the essential oil of Psidium
guineense Sw and spathulenol. J Ethnopharmacol 210:351–358
Oliveira VB, Yamada LT, Fagg CW, Brandão MGL (2012) Native foods from Brazilian biodiver-
sity as a source of bioactive compounds. Food Res Int 48:170–179
Oliveira MMM, Brugnera DF, Piccoli RH (2013) Essential oils of thyme and Rosemary in the
control of Listeria monocytogenes in raw beef. Braz J Microbiol 44:1181–1188
Ormeno E, Fernandez C (2012) Effect of soil nutrient on production and diversity of volatile
terpenoids from plants. Curr Bioact Compd 8:71–79
Pal PK, Mahajan M, Agnihotri VK (2016) Foliar application of plant nutrients and kinetin modi-
fies growth and essential oil profile in Rosa damascena under acidic conditions. Acta Physiol
Plant 38:176
Pandotra P, Gupta AP, Gandhiram et al (2013) Genetic and chemo-divergence in eighteen core
collection of Zingiber officinale from North-West Himalayas. Sci Hortic 160:283–291
Park YK, Paredes-Guzman JF, Aguiar CL et al (2004) Chemical constituents in Baccharis dracun-
culifolia as the main botanical origin of southeastern Brazilian propolis. J Agric Food Chem
52:1100–1103
Park MJ, Gwak KS, Yang I et al (2009) Effect of citral, eugenol, nerolidol and alpha-terpineol on
the ultrastructural changes of Trichophyton mentagrophytes. Fitoterapia 80:290–296
Pascual-Villalobos MJ, Ballesta-Acosta MC (2003) Chemical variation in an Ocimum basilicum
germplasm collection and activity of the essential oils on Callosobruchus maculatus. Biochem
Syst Ecol 31:673–679
Passos GF, Fernandes ES, da Cunha FM et al (2007) Anti-inflammatory and anti-allergic prop-
erties of the essential oil and active compounds from Cordia verbenacea. J Ethnopharmacol
110:323–333
2 Factors Influencing the Production and Chemical Composition of Essential Oils… 45
Pavarini DP, Nogueira EF, Callejon DR et al (2013) Novel bisabolane derivative from “arnica-da-
serra” (Vernonieae: Asteraceae) reduces pro-nociceptive cytokines levels in LPS-stimulated rat
macrophages. J Ethnopharmacol 148:993–998
Perigo CV, Torres RB, Bernacci LC et al (2016) The chemical composition and antibacterial activ-
ity of eleven Piper species from distinct rainforest areas in Southeastern Brazil. Ind Crop Prod
94:528–539
Prasad G, Kuman A, Singh AK, Bhattacharya AK et al (1986) Antimicrobial activity of essential
oils of some Ocimum species and clove oil. Fitoterapia 57:429–432
Primack RB, Rodrigues E (2001) Biologia da conservação. Planta, Londrina
Queiroz VS (2012) Avaliação in vitro do efeito dose-resposta da nanoemulsão do extrato etanólico
de Lychnophora pinaster sobre células planctônicas e biofilme de Streptococcus mutans e sobre
a desmineralização do esmalte dental ao redor de braquetes ortodônticos. Thesis, Universidade
Estadual de Campinas
Rana IS, Rana AS, Rajak RC (2011) Evaluation of antifungal activity in essential oil of the
Syzygium aromaticum (L.) by extraction, purification and analysis of its main component euge-
nol. Braz J Microbiol 42:1269–1277
Rehman R, Hanif MA (2016) Biosynthetic factories of essential oils: the aromatic plants. Nat Prod
Chem Res 04(04)
Reigosa M, Gomes AS, Ferreira AG, Borghetti F (2013) Allelopathic research in Brazil. Acta
Botanica Brasilica 27(4):629–646
Richards EJ (2006) Inherited epigenetic variation-revisiting soft inheritance. Mycorrhiza
7:395–401
Rodrigues VEG (1988) Levantamento florístico e etnobotânico de plantas medicinais dos cerrados
na região do Alto Rio Grande – Minas Gerais. Dissertation, Universidade Federal de Lavras
Rice EL (1984) Alelopatia. Academic Press, New York
Saggiorato AG, Gaio I, Treichel H et al (2012) Antifungal activity of basil essential oil (Ocimum
basilicum L.): evaluation in vitro and on an Italian-type sausage surface. Food Bioproc Technol
5:378
Scavroni J, Boaro CSF, Marques MOM, Ferreira LC (2005) Yield and composition of the essential
oil of Mentha piperita L. (Lamiaceae) grown with biosolid. Braz J Plant Physiol 17:345–352
Schilmiller AL, Last RL, Pichersky E (2008) Harnessing plant trichome biochemistry for the
production of useful compounds. Plant J 54:702–711
Semir J (1991) Revisão taxonômica de Lychnophora Mart. (Vernoniaceae: Compositae). Thesis,
Universidade de Campinas
Semir J, Rezende AR, Monge M, Lopes NP (2011) As arnicas endêmicas das Serras do Brasil:
Uma visão sobre a biologia e a química das espécies de Lychnophora (Asteraceae). UFOP,
Ouro Branco (MG)
Sforcin JM, Souza JPB, Silva Filho AA et al (2012) Baccharis dracunculifolia: Uma das principais
fontes vegetais da própolis brasileira. Editora UNESP, São Paulo
Shao M, Czapiewski KV, Heiden AC et al (2001) Volatile organic compound emissions from Scots
pine: mechanisms and description by algorithms. J Geophys Res 106:483–491
Shao H, Guo Q, Chu L et al (2007) Understanding molecular mechanism of higher plant plasticity
under abiotic stress. Colloids Surf B: Biointerfaces 54:37–45
Shulaev V, Cortes D, Miller G, Mittler R (2008) Metabolomics for plant stress response. Physiol
Plant 132:199–208
Sienkiewicz M, Lysakowska M, Denys P, Kowalczyk E (2012) The antimicrobial activity of
Thyme essential oil against multidrug resistant clinical bacterial strains. Microb Drug Resist
18:137–148
Sifola MI, Barbieri G (2006) Growth, yield and essential oil content of three cultivars of basil
grown under different levels of nitrogen in the field. Sci Hortic 108:408–413
Silva PSS (2013) Caracterização da composição química dos óleos essenciais de Lychnophora
pinaster Mart. em função da sazonalidade Dissertation, Universidade Estadual Paulista Júlio
de Mesquita Filho.
46 C. S. F. Boaro et al.
Vieira MAR, Marques MOM, Haber LL et al (2017) Perfil do óleo essencial de folhas de
Lychnophora ericoides Mart coletadas em duas localidades de Minas Gerais. In: 9° Simpósio
Brasileiro de Óleos Essenciais
Voirin B, Brun N, Bayet C (1990) Effects of daylength on the monoterpene composition of leaves
of Mentha x piperita. Phytochemistry 29:749–755
Wang Y, Wang X, Yang Z et al (2012) Menthol inhibits the proliferation and motility of prostate
cancer DU145 cells. Pathol Oncol Res 18:903–910
Wink M (2016) Secondary metabolites, the role in plant diversification of Encyclopedia of. Evol
Biol 4:1–9
Ye Y, Liang X, Chen Y et al (2014) Carbon, nitrogen and phosphorus accumulation and partition-
ing, and C: N: P stoichiometry in late-season rice under different water and nitrogen manage-
ments. PLoS One 9:7
Zucchi MI (2009) Diversidade genética em espécies medicinais. http://www.infobibos.com/
Artigos/2009_4/DiversidadeGenetica/index.htm. Accessed: 11/6/2018
Chapter 3
Hedychium Essential Oils: Composition
and Uses
Hamidou F. Sakhanokho and Kanniah Rajasekaran
3.1 Introduction
H. F. Sakhanokho
USDA-ARS, Thad Cochran Southern Horticultural Laboratory, Poplarville, MS, USA
K. Rajasekaran (*)
USDA-ARS, Southern Regional Research Center, New Orleans, LA, USA
e-mail: [email protected]
Fig. 3.1 Hedychium species are used for multiple purposes, one of which is their ornamental
value. Hedychium species are gaining popularity as ornamental plants because of their attractive
foliage, diverse and showy flowers, and sweet fragrance. (a) Hedychium “Kinkaku”; (b) Hedychium
“Dr. Moy”; (c) Hedychium “Betty Ho”; (d) H. anguistifolium; (e) H. coronarium; (f) H. coccineum;
(g) Hedychium “Daniel Weeks”; (h) H. gardnerianum; (i) H. longicornutum; (j) Hedychium
“Orange Brush”
In addition to the beneficial traits mentioned above, Hedychium spices, like many
members of Zingiberaceae, are widely used in ethnomedicine to treat various
ailments including nausea, asthma, flu, diarrhea, snake bites, and leishmaniasis
(Chen et al. 2008; Valadeau et al. 2009; Tushar et al. 2010; Tiuman et al. 2011).
Further, the essential oils extracted from various Hedychium plant parts have shown
antimicrobial activities in various studies, so they offer the potential to be used as
safer alternatives to synthetic antibiotics, antifungals, and insecticides. For example,
essential oils in the leaves and flowers of H. gardnerianum have been found to have
antimicrobial activity against certain bacteria, namely, Staphylococcus aureus and
S. epidermis (Medeiros et al. 2003), while Gopanraj et al. (2005) reported
antibacterial activity of the essential oil from the rhizomes of H. larsenii against
both Gram-positive and Gram-negative bacteria. Others have reported antifungal,
insecticidal, and antioxidant properties of essential oils from various Hedychium
species (Rajasekaran et al. 2012; Sakhanokho et al. 2013; Ray et al. 2018).
Essential oils can be defined as a mixture of volatile and natural substances, charac-
terized by a strong odor and produced by aromatic plants as secondary metabolites
(Sá et al. 2013). Increasingly, plant essential oils are recognized as important sources
of biopesticides, against which insects and microbes do not acquire induced resis-
tance and have limited toxic effects on human or animal health and non-target
organisms (AlShebly et al. 2017). The antimicrobial properties of Hedychium
essential oils, in particular, have been well documented, and their use as “biocides”
is gaining popularity (Sakhanokho et al. 2013). Listed in Table 3.1 are Hedychium
3 Hedychium Essential Oils: Composition and Uses 51
Table 3.1 (continued)
Compound percentage (w/w)
Taxa Major compound Leaves Flowers Rhizomes Stems Reference
Hedychium Linalool 29.3 Prakash et al.
coronarium Limonene 20.3 (2010)
trans-Meta- 12.9
mentha,2,8-diene
γ-Terpinene 8.9
10-Epi-γ-eudismol 3.8
Hedychium 1,8-Cineole 17.6 Prakash et al.
spicatum α-Eudesmol 17 (2010)
10-Epi-γ-eudismol 9.7
δ-Cadinene 7.5
Eugenol 6.9
Germacrene D-4-ol 6.8
γ-Cadinene 5.4
Hedychium β-Pinene 33.9 23.0 Ho (2011)
coronarium α-Pinene 14.7
1,8-Cineole 13.3 37.3
r-Elemene 11.0
Carotol 9.1
α-Terpineol 10.4
α-Pinene 9.9
Hedychium β-Pinene 46.9 41.5 Miranda et al.
coronarium 1,8-Cineole 23.6 (2014)
α-Pinene 19.2 13.1
β-Caryophyllene 13.2
Hedychium α-Pinene 52.5 5.0 Van Thanh
stenopetalum β-Pinene 31.8 et al. (2014)
Linalool 45.2
(E)-Nerolidol 8.7
Hedychium β-Pinene 20.0 23.6 Van Thanh
coronarium Linalool 15.8 et al. (2014)
1,8-Cineole 10.7
α-Pinene 10.1
α-Terpineol 8.6
α-Humulene 17.1
β-Caryophyllene 13.0
Hedychium β-Pinene 22.5 21.8 11.2 Van Thanh
flavum α-Humulene 15.7 18.9 et al. (2014)
β-Caryophyllene 10.4 11.8
Linalool 17.5
1,8-Cineole 13.5
(continued)
3 Hedychium Essential Oils: Composition and Uses 53
Table 3.1 (continued)
Compound percentage (w/w)
Taxa Major compound Leaves Flowers Rhizomes Stems Reference
Hedychium (E)-Nerolidol 15.9 Van Thanh
ellipticum β-Pinene 11.8 11.0 et al. (2014)
Bomyl acetate 9.2
1,8-Cineole 40.8
α-Pinene 18.3
Hedychium 1,8-Cineole 37.7 Abdo et al.
malayanum β-Pinene 35.2 (2015)
α-Pinene 10.9
Hedychium β-Pinene 18.3 Thomas and
forrestii β-Linalool 17.8 Mani (2016)
1,8-Cineole 12.0
4-Terpineol 5.5
Hedychium Linalool 83.01 Kumar et al.
aurantiacum Limonene 4.81 (2017)
α-Terpinene 2.69
trans-Linalool oxide 1.55
cis-Linalool oxide 1.53
Hedychium Linalool 45.6 Thomas and
matthewiii β-Pinene 6.5 Mani (2018)
Camphene 3.3
α-Pinene 2.5
Hedychium α-Fenchyl acetate 45.85 Hartati et al.
roxburghii Alloaromadendrene 8.83 (2015)
β-Maaliene 4.88
Spathulenol 4.42
Hedychium (+)-Linalool 80.6 Pant et al.
aurantiacum (1992)
Hedychium β-Caryophyllene 43.0 Dos Santos
coronarium Caryophyllene oxide 12.1 et al. (2010)
β-Pinene 11.6 16.7
1,8-Cineole 34.8
α-Terpineol 13.1
Hedychium 1,8-Cineole 30.84 Semwal et al.
spicatum β-Eudesmol 14.50 (2015)
β-Pinene 9.24
Limonene 6.42
Linalool 5.29
Hedychium 1,8-Cineole 42 Sakhanokho
“Dr. Moy” et al. (2013)
Hedychium Linalool 56 Sakhanokho
forrestii et al. (2013)
(continued)
54 H. F. Sakhanokho and K. Rajasekaran
Table 3.1 (continued)
Compound percentage (w/w)
Taxa Major compound Leaves Flowers Rhizomes Stems Reference
Hedychium α-Pinene 17 Sakhanokho
“Tai Emperor” et al. (2013)
Hedychium β-Pinene 31 Sakhanokho
bousigonianum et al. (2013)
Hedychium (E)-Nerolidol 20 Sakhanokho
“Dave Case” et al. (2013)
Hedychium α-Cadinol 26.22 Medeiros et al.
gardnerianum α-Pinene 18.37 (2003)
β-Pinene 14.53
Hedychium Linalool 62.26 Gopanraj et al.
larsenii 1,8-Cineole 14.41 (2005)
Hedychium ar-Curcumene 28.6 AlShebly et al.
larsenii epi-β-Bisabolol 10.3 (2017)
taxa along with the major constituents of their essential oils, but this list is far from
being exhaustive. In addition to published chemical constituents as cited in Table 3.1,
other minor chemical compounds were also identified but not listed here. Plant parts
used for essential oil extraction include leaves, stems (pseudo-stems), flowers, roots,
and rhizomes (Arruda et al. 2012; Sakhanokho et al. 2013; Verma and Padalia
2010). The rhizomes appear to be the plant material most often used for Hedychium
essential oil extraction. This is most likely because once Hedychium plants are
grown and well established, their rhizomes offer readily available vegetal parts
throughout the year. Among the taxa listed in Table 3.1 are the variegated Hedychium
cultivar “Dr. Moy” (Fig. 3.1b), H. coronarium (Fig. 3.1e), H. coccineum (Fig. 3.1f),
and H. gardnerianum (Fig. 3.1h).
Monoterpenes and sesquiterpenes constitute the main compounds as reported in
most of the studies on Hedychium essential oils (Table 3.1). This is particularly true
for essential oil studies in H. coronarium where the monoterpene 1,8-cineole is
ubiquitous and found in both rhizomes and leaves. In an essential oil study involving
19 Hedychium genotypes, the compound 1,8-cineole was, by far, the most ubiquitous
constituent of the oils being found in 16 out of the 19 Hedychium genotypes. In 13
of those genotypes, 1,8-cineol was the dominant constituent (Sakhanokho et al.
2013). Other monoterpene compounds found by the same authors included linalool
(<0.1–56%), α-pinene (3–17%), and β-pinene (4–31%) followed by sesquiterpene
constituents such as (E)-nerolidol (0.1–20%). Further, Abdo et al. (2015) reported
that 99% of the essential oil compounds found in Hedychium malayanum rhizomes
were monoterpenes, including 1,8-cineole, β-pinene, and α-pinene. Similarly,
Gopanraj et al. (2005) found that 99% of the essential oils found in Hedychium
larsenii were monoterpenes, with linalool and 1,8-cineole as major constituents.
Recently, however, AlShebly et al. (2017) found that, instead of monoterpene com-
pounds, the major compounds found in essential oils of the same species, H. larsenii,
were the sesquiterpene compounds ar-curcume (28.6%) and epi-β-bisabolol (10.3%).
3 Hedychium Essential Oils: Composition and Uses 55
Differences in oil constituents of the same Hedychium species have been reported
before (Verma and Padalia 2010; Ray et al. 2018), and these differences are attrib-
uted to various factors. Chemical composition of the essential oil of the same plant
species can vary depending on factors such as genotype, season, vegetal parts, and
environment. For example, Zheljazkov et al. (2008) reported differences in oil con-
stituents of the same cultivars of two other species, Ocimum basillicum L. and O.
sanctum L., grown in two different locations and attributed these differences to dif-
ferent environmental conditions such as temperature, soil characteristics, and pro-
duction system at the two locations of their study. Ray et al. (2018) studied the
chemical diversity, antioxidant, and antimicrobial activities of the essential oils
from various Indian populations of Hedychium coronarium and found that
geographic origin greatly influenced not only the chemical composition of essential
oils but also their associated bioactivities. Furthermore, production of a particular
type of oil is highly influenced by the physiology of plant, which undergoes, for
example, crucial changes during the transition from the vegetative stage to flowering
(Sangwan et al. 2001; Argyropoulou et al. 2007).
Table 3.2 (continued)
Taxa Organism Efficacya Reference
Hedychium larsenii Bacillus cereus + Gopanraj et al.
Bacillus subtilis + (2005)
Staphylococcus aureus +
Escherichia coli +
Klebsiella pneumoniae −
Proteus vulgaris +
Pseudomonas aeruginosa +
Pseudomonas fluorescens +
Salmonella typhii +
Serratia marcescens +
Hedychium spicatum Pediculus humanus capitis + Jadhav et al. (2007)
Pthirus pubis +
Pediculus humanus +
Hedychium forrestii Stephanitis pyrioides + Sakhanokho et al.
(2013)
Hedychium elatum Stephanitis pyrioides +
Hedychium bousigonianum Stephanitis pyrioides +
Hedychium “Tai Golden Stephanitis pyrioides −
Goddes”
Hedychium bousigonianum First instar larvae of Aedes aegypti + Sakhanokho et al.
(2013)
Hedychium coccineum First instar larvae of Aedes aegypti +
Hedychium “Kinkaku” First instar larvae of Aedes aegypti +
Hedychium “Tai Golden First instar larvae of Aedes aegypti +
Goddess”
Hedychium forrestii Colletotrichum gloeosporioides − Sakhanokho et al.
Colletotrichum fragariae − (2013)
Colletotrichum acutatum −
Hedychium elatum Colletotrichum gloeosporioides −
Colletotrichum fragariae −
Colletotrichum acutatum −
Hedychium gardnerianum Staphylococcus aureus + Medeiros et al.
Staphylococcus epidermis + (2003)
Pseudomonas aeruginosa −
Hedychium forrestii Aspergillus flavus + Rajasekaran et al.
Fusarium verticillioides + (2012)
Hedychium elatum Aspergillus flavus + Rajasekaran et al.
Fusarium verticillioides + (2012)
Hedychium “Tai Monarch” Aspergillus flavus + Rajasekaran et al.
Fusarium verticillioides + (2012)
a
The + sign indicates the essential oil compound is effective or partially effective against the
targeted organism; the − sign signifies that the essential oil compound has no effect on the targeted
organism
58 H. F. Sakhanokho and K. Rajasekaran
potent antimicrobial activity than the individual main components mixed together,
proving the significant role played by the minor compounds in the antimicrobial
effectiveness of essential oils (Marino et al. 2001; Delaquis et al. 2002; Gill et al.
2002; Mourey and Canillac 2002; Ait-Ouazzou et al. 2012).
3.4 Conclusions
Hedychium species are multiple-purpose plants that are generally grown as orna-
mental plants, but they are also used for their industrial (perfumery, manufacturing
paper) and medicinal (antimicrobial and oxidant properties) properties. In addition,
Hedychium extracts are widely used in traditional medicine to treat a multitude of
ailments. Furthermore, Hedychium essential oils have been shown to inhibit fungal
growth and aflatoxin secretion, so they can potentially be used in formulations of
plant-based preservatives for improvement of stored foods.
References
Abdo MY, Ahmad WYW, Din LB, Ibrahim N (2015) Composition of Hedychium malayanum
rhizome oil and its antioxidant activity. Malays J Anal Sci 19:1147–1155
Ahmad BF, Jantan BI, Jalil J (2004) Constituent of the rhizome oil of Hedychium cylindricum Ridl.
J Essent Oil Res 16:299–301
Ait-Ouazzou A, Loran S, Arakrak A, Laglaoui A, Rota C, Herrera A, Pagán R, Conchello P
(2012) Evaluation of the chemical composition and antimicrobial activity of Mentha pule-
gium, Juniperus phoenicea, and Cyperus longus essential oils from Morocco. Food Res Int
45:313–319
AlShebly MM, AlQahtani FS, Govindarajan M, Gopinah K, Vijayan P, Benelli G (2017) Toxicity
of ar-curcumene and epi-ß-bisabolol from Hedychium larsenii (Zingiberaceae) essential oil
on malaria, chikungunya and St. Louis encephalitis mosquito vectors. Ecotoxicol Environ Saf
137:149–157
Argyropoulou C, Daferera D, Tarantilis PA, Fasseas C, Polissiou M (2007) Chemical composition
of the essential oil from leaves of Lippia citriodora H.B.K. (Verbenaceae) at two developmen-
tal stages. Biochem Syst Ecol 35:831–837
Arruda M, Viana H, Rainha N, Neng NR, Rosa JS, Nogueira JMF, Barreto MC (2012) Anti-
acetylcholinesterase and antioxidant activity of essential oils from Hedychium gardnerianum
Sheppard ex Ker-Gawl. Molecules 17:3082–3092
Branney TME (2005) Hardy gingers: including Hedychium, Roscocea, and Zingiber,
Royal Horticultural Society Plant Collector Guide. Timber Press, Portland, 267p. ISBN:
0881926779
Chen IN, Chang CC, Ng CC, Wang CY, Shyu YT, Chang TL (2008) Antioxidant and antimicrobial
activity of Zingiberaceae plants in Taiwan. Plant Foods Hum Nutr 63:15–20
Chimnoi N, Pisutiaroenpong S, Ngiwsara L, Dechtrirut D, Chokchaichamnanki D,
Khunnawutmanotham N, Mahidol C, Techasakul S (2008) Labdane diterpenes from rhizomes
of Hedychium coronarium. Nat Prod Res 22:1249–1255
Delaquis PJ, Stanich K, Girard B, Mazza G (2002) Antimicrobial activity of individual and
mixed fractions of dill, cilantro, coriander and eucalyptus essential oils. Int J Food Microbiol
74:101–109
3 Hedychium Essential Oils: Composition and Uses 59
Ding HB, Bin Y, Zhou SS, Li R, Maw MB, Kyaw WM, Tan YH (2018) Hedychium putaoense
(Zingiberaceae), a new species from Putao, Kachin State, Northern Myanmar. PhytoKeys
94:51–57
dos Santos BCB, Barata LES, Marques FA, Baroni ACM, Kanos BAC, de Oliveira PR, Guerrero
PG Jr (2010) Composition of leaf and rhizome essential oils of Hedychium coronarium Koen.
from Brazil. J Essent Oil Res 22:305–306
Gill AO, Delaquis P, Russo P, Holley RA (2002) Evaluation of antilisterial action of cilantro oil on
vacuum packed ham. Int J Food Microbiol 73:83–92
Gopanraj G, Dan M, Shihuraj S, Sethuraman MG, George V (2005) Chemical composition and
antibacterial activity of the rhizome oil of Hedychium larsenii. Acta Pharma 55:315–320
Gurib-Fakim A, Maudarbaccus N, Leach D, Doimo L, Vohlmuth H (2002) Essential oil compo-
sition of Zingiberaceae species from Mauritius. J Essent Oil Res 14:271–273
Hartati R, Suganda AG, Fidrianny I (2015) Chemical composition and antimicrobial activity of
diterpene and essential oils of Hedychium roxburghii Blume rhizome. Asian J Pharm Clin Res
8:209–214
Ho J-C (2011) Antimicrobial, mosquito larvicidal and antioxidant properties of the leaf and rhi-
zome of Hedychium coronarium. J Chin Chem Soc 58:563–567
Jadhav V, Kore A, Kadam VJ (2007) In-vitro pediculicidal activity of Hedychium spicatum essen-
tial oil. Fitoterapia 78:470–473
Kumar R, Prakash O, Singh SP, Pant AK, Isidorov V, Szczepaniak L (2017) Chemical composi-
tion, antioxidant and myorelaxant activity of essential oil of Hedychium aurantiacum. Asian
J Chem 29:2587–2591
Lechat-Vahirua I, François P, Menut C, Lamaty G, Bessiere J-M (1993) Aromatic plants of French
Polynesia. I. Constituents of the essential oils of rhizomes of three Zingiberaceae: Zingiber zerum-
bet Smith, Hedychium coronarium Koenig and Etlingera cevuga Smith. J Essen Oil Res 5:55–59
Marino M, Bersani C, Comi G (2001) Impedance measurements to study the antimicrobial activity
of essential oils from Lamiacea and Compositae. Int J Food Microbiol 67:187–195
Medeiros J, Campos LB, Mendonca SC, Davin LB, Lewis NG (2003) Composition and antimicro-
bial activity of the essential oils from invasive species of the Azores, Hedychium gardnerianum
and Pittosporum undulatum. Phytochemistry 64:561–565
Miranda CASF, Cardoso MG, Mansanares ME, Gomes MS, Marcussi S (2014) Preliminary
assessment of Hedychium coronarium essential oil on fibrinogenolytic and coagulant activity
induced by Bothrops and Lachesis snake venoms. J Venom Anim Toxins Incl Trop Dis 20:39
Mourey A, Canillac N (2002) Anti-Listeria monocytogenes activity of essential oils components of
conifers. Food Control 13:289–292
Mukherjee I (1970) Chromosome studies of some species of Hedychium. Bot Mag Tokyo
83:237–241
Nazzoro F, Fratianni F, De Martino L, Coppola R, De Feo V (2013) Effect of essential oils on
pathogenic bacteria. Pharmaceuticals 6:1461–1474
Pant AK, Mathela CS, Dev V, Bottini AT (1992) Rhizome essential oil of Hedychium aurantiacum
(Zingiberaceae), a potential source of (+)-linalool. J Essent Oil Res 4:129–131
Pawar VC, Thaker VS (2006) In vitro efficacy of 75 essential oils against Aspergillus niger.
Mycoses 49:316–323
Prakash O, Rajput M, Kumar M, Pant AK (2010) Chemical composition and antibacterial activity
of rhizome oils from Hedychium coronarium Koenig and Hedychium spicatum Buch-Ham.
J Essent Oil Bearing Plants 13:250–259
Rajasekaran K, Sakhanokho HF, Tabanca N (2012) Antifungal activities of Hedychium essential
oils and plant extracts against mycotoxigenic fungi. J Crop Improv 26:389–396
Ray A, Dash B, Sahoo A, Nasim N, Panda PC, Patnaik J, Ghosh B, Nayak S, Kar B (2017a)
Assessment of the terpenic composition of Hedychium coranarium oil from eastern India. Ind
Crop Prod 97:49–55
Ray A, Jena S, Kar B, Patnaik J, Panda PC, Nayak S (2017b) Chemical composition and antioxi-
dant activities of essential oil Hedychium greenii and Hedychium gracile from India. Nat Prod
Res:1–4. https://doi.org/10.1080/14786419.2017.1416384
60 H. F. Sakhanokho and K. Rajasekaran
Ray A, Jena S, Dash B, Kar B, Halder T, Chatterjee T, Ghos B, Panda PC, Nayak S, Mahapatra
N (2018) Chemical diversity, antioxidant and antimicrobial activities of the essential oils from
Indian populations of Hedychium coronarium Koen. Ind Crop Prod 112:353–362
Rodrigues DF, Velasquez AMA, Cavaleiro C, Salgueiro L, Martins GZ, Magalhaes NO, Martins
MBG, Cicarelli RMB, Moreira RRD (2013) Chemical composition and trypanocidal activity
of the essential oils from Hedychium coronarium J. Koenig (Zingiberaceae). ISRN Infect Dis
2013:1–6. https://doi.org/10.5402/2013/639275
Sá RCS, Andrade LN, Sousa DP (2013) A review on ant-inflammatory activity of monoterpenes.
Molecules 18:1227–1254
Sakhanokho HF, Sampson BJ, Tabanca N, Wedge DE, Demirci B, Baser KHC, Bernier UR,
Tsikolia M, Agramonte NM, Becnel JJ, Chen J, Rajasekaran K, Spiers JM (2013) Chemical
composition, antifungal and insecticidal activities of Hedychium essential oils. Molecules
18:4308–4327
Sangwan NS, Farroqi AHA, Shabih F, Sangwan RS (2001) Regulation of essential oil production
in plants. Plant Growth Regul 34:3–21
Sarangthem N, Talukdar NC, Thongan B (2013) Collection and evaluation of Hedychium species
of Manipur, Northeast India. Genet Resour Crop Evol 60:13–21
Semwal RB, Semwal DK, Mishra SP, Semwal R (2015) Chemical composition and antibacte-
rial potential of essential oils from Artemisia capillaris, Artemisia nilagirica, Citrus limon,
Cymbopogon flexuous, Hedychium spicatum and Ocimum tenuiflorum. Nat Prod J 5:199–205
Thomas S, Mani B (2016) Chemical composition, antibacterial and antioxidant properties of
essential oil from rhizomes of Hedychium forrestii var. planiense Sanoj and M. Sabu. Ind
J Pharm Sci 78:452–457
Thomas S, Mani B (2018) Composition, antibacterial and anti-oxidant potentials of the essential
oil of Hedychium matthewii. Bangladesh J Pharmacol 12:173–179
Tiuman TS, Santos AO, Ueda-Nakamura T, Filho BPD, Nakamura CV (2011) Recent advances in
leishmaniasis treatment. Int J Infect Dis 15:e525–e532
Trombetta D, Castelli F, Sarpietro MG, Venuti V, Cristani M, Daniele C, Saja A, Mazzanti G,
Bisignano G (2005) Mechanisms of antibacterial action of three monoterpenes. Antimicrob
Agents Chemother 49:2474–2478
Tushar BS, Sarma GC, Rangan L (2010) Ethnomedical uses of zingiberaceous plants of Northeast
India. J Ethnopharmacol 132:286–296
Valadeau C, Pabon A, Deharo E, Alban-Castillo J, Estevez Y, Lores FA, Rojas R, Gamboa D,
Sauvain M, Castillo D, Bourdy G (2009) Medicinal plants from the Yanesha (Peru): evalu-
ation of the leishmanicidal and antimalarial activity of selected extracts. J Ethnopharmacol
123:413–422
Van Thanh B, Dai DN, Thang TD, Binh NQ, Anh LDN, Ogunwande IA (2014) Composition
of essential oils of four Hedychium species from Vietnam. Chem Cent J 8:54. https://doi.
org/10.1186/s13065-014-0054-3
Verma RS, Padalia RC (2010) Comparative essential oil composition of different vegetative parts
of Hedychium spicatum Smith from Uttarakhand, India. Int J Green Pharm 4:292–295
Weyerstahl P, Marshall H, Schneider S (1995) Constituents of the essential oil from the rhizomes
of Hedychium acuminatum Roscoe. Flavour Fragr J 10:179–185
Wood T (1999) Ginger lilies. The American Gardener 78:40–45
Zheljazkov V, Cantrell CL, Tekwani B, Khan SI (2008) Content, composition, and bioactivity of the
essential of three basil genotypes as a function of harvesting. J Agric Food Chem 56:380–385
Chapter 4
The Essential Oils of the Burseraceae
Abbreviations
DCM Dichloromethane
HD Hydrodistillation
IC50 Median inhibitory concentration
LC50 Median lethal concentration
LD50 Median lethal dose
MeOH Methanol
MIC Minimum inhibitory concentration
MRSA Methicillin-resistant Staphylococcus aureus
SAFE Solvent-assisted flavor evaporation
SC CO2 Supercritical carbon dioxide extraction
SD Steam distillation
SPME Solid-phase micro extraction
A. DeCarlo
Aromatic Plant Research Center, Lehi, Utah, USA
Department of Environmental Studies, Saint Michael’s College, Colchester, VT, USA
e-mail: [email protected]
N. S. Dosoky · P. Satyal · A. Sorensen
Aromatic Plant Research Center, Lehi, Utah, USA
dōTERRA International, Pleasant Grove, UT, USA
e-mail: [email protected]; [email protected];
[email protected]
W. N. Setzer (*)
Aromatic Plant Research Center, Lehi, Utah, USA
Department of Chemistry, University of Alabama in Huntsville, Huntsville, AL, USA
e-mail: [email protected]
The genus Bursera comprises around 100 species of deciduous aromatic trees and
shrubs distributed from southern United States through Peru (Espinosa et al. 2006;
Mabberley 2008; De-Nova et al. 2011; Gigliarelli et al. 2015). Bursera species have
played a prominent role in the ethnomedicine throughout their ranges (Morton
1981; Duke et al. 2009). Members of Bursera produce oleoresins and essential oils
that not only serve as protection of the plants against herbivory and infection but can
also be attributed to the numerous medicinal properties of this genus, including
antimicrobial, antineoplastic, anti-inflammatory, and analgesic (Noguera et al.
2004; Murthy et al. 2016). The resins, known as “copal,” have been burned as
incense since pre-Columbian times. Bursera resins are also used for making
ointments, treating scorpion bites, relieving cold symptoms, and as a remedy for
headaches (Peters et al. 2003). The essential oil compositions and biological
activities of Bursera species are summarized in Table 4.1.
Table 4.1 Chemical compositions and biological activities of Bursera essential oils
Bursera speciesa Collection site Essential oil Major components (>5%) Bioactivity of essential oil Reference
B. aloexylon (Schiede ex Guerrero, Mexico Bark (SD) Linalool (49.3%), α-terpineol – Zúñiga et al. (2005)
Schltdl.) Engl. [syn. (20.2%), linalyl anthranilate
Bursera linanoe (La (14.5%), neryl acetate
Llave) Rzed., Calderón & (5.1%)
Medina] Zapotitlan, Puebla, Leaf (DCM extract) Linalyl acetate (92.2%) – Becerra and Noge
Mexico (2010)
Tucson, Arizona Leaf (DCM extract) (R)-(−)-Linalyl acetate – Noge et al. (2010)
(greenhouse) (57.6%), (S)-(−)-germacrene
D (39.3%)
Campinas, SP Brazil Leaf (HD) Linalool (96.7%) Antibacterial, broth Queiroga et al.
microdilution assay (2007)
4 The Essential Oils of the Burseraceae
Bursera speciesa Collection site Essential oil Major components (>5%) Bioactivity of essential oil Reference
Trelawny, Jamaica Leaf (HD) Nonane (14.7%), α-copaene – Junor et al. (2010b)
(15.8%), β-caryophyllene
(21.7%), δ-cadinene
(11.3%), viridiflorol (5.9%)
B. bipinnata (DC.) Engl. Commercial Oleoresin (HD) α-Copaene (14.5%), – Case et al. (2003)
β-bourbonene (6.1%),
β-caryophyllene (8.5%),
germacrene D (13.8%),
spathulenol (5.1%)
B. chemapodicta Rzed. & Guerrero, Mexico Leaf (SPME) Heptane (22.5%), 2-heptanol – Evans and Becerra
Evangelina Ortiz (26.4%), 2-heptyl acetate (2006)
(40.0%)
Guerrero, Mexico Twig (SPME) Heptane (19.4%), nonane – Evans and Becerra
(6.0%), 4-methyl-3-hexyl (2006)
acetate (15%), 2-heptyl
acetate (51.0%)
B. copallifera (DC) Tucson, Arizona Leaf (DCM extract) β-Caryophyllene (9.6%), – Noge and Becerra
Bullock (greenhouse) α-humulene (12.5%), (2009)
germacrene D (56.2%),
bicyclogermacrene (6.2%)
B. delpechiana Poisson, Bangalore, India Leaf (DCM extract) Linalyl acetate (90.3%) – Becerra and Noge
Henri Louis ex Engl. [syn (2010)
B. citronella McVaugh &
Rzed.]
B. excelsa (Kunth) Engl. Tucson, Arizona Leaf (DCM extract) β-Caryophyllene (15.0%), – Noge and Becerra
(greenhouse) germacrene D (50.5%), (2009)
bicyclogermacrene (8.8%)
A. DeCarlo et al.
B. fagaroides var. purpusii Tucson, Arizona Leaf (DCM extract) α-Pinene (67.8%), β-pinene – Noge and Becerra
(Brandegee) McVaugh & (greenhouse) (5.7%), germacrene D (2009)
Rzed. (15.1%)
B. glabrifolia (Kunth) Guerrero, Mexico Bark (SD) α-Thujene (7.4%), limonene – (Zúñiga et al. (2005)
Engl. (13.4%), terpinen-4-ol
(34.5%), α-terpineol
(31.6%), verbenone (7.5%)
B. grandifolia (Schltdl.) Guerrero, Mexico Bark (SD) α-Thujene (23.5%), Anti-inflammatory (mouse Zúñiga et al. (2005)
Engl. limonene (64.0%) ear edema, 2.0% inhibition
with a dose of 2.5 μg ear−1)
B. graveolens (Kunth) Limones, Ecuador Fruit (HD) α-Phellandrene (37.6%), Acaricidal (Rhipicephalus Rey-Valeirón et al.
Triana & Planch. limonene (49.9%), (Boophilus) microplus (2017)
menthofuran (6.1%) larvae, IC50 0.87%)
4 The Essential Oils of the Burseraceae
Havana, Cuba Leaf (HD) Limonene (26.5%), Antiprotozoal (Leishmania Monzote et al.
(E)-β-ocimene (13.0%), amazonensis amastigotes, (2012)
menthofuran (5.1%), IC50 36.7 μg mL−1)
β-elemene (14.1%)
San José de Ancon, Stem (HD) Viridiflorol (70.8%), – Manzano Santana
Ecuador α-cadinol (5.5%) et al. (2009)
Piura, Peru Wood (SD) Limonene (9.1%), – Yukawa et al. (2006)
α-terpineol (8.1%),
β-bisabolene (5.7%)
Puerto Lopez, Wood (SD) Limonene (58.6%), – Young et al. (2007)
Ecuador menthofuran (6.6%),
α-terpineol (10.9%)
Commercial (Young Wood (HD) Limonene (67%), α-terpineol – Satyal (unpublished)
Living Essential (10%)
Oils, Lehi, Utah)
Commercial (Santoil Oleoresin (HD) Limonene (41%), – Satyal (unpublished)
S.A., Quito, menthofuran (35%),
Ecuador) germacrene D (16%)
65
(continued)
Table 4.1 (continued)
66
Bursera speciesa Collection site Essential oil Major components (>5%) Bioactivity of essential oil Reference
B. hollickii Fawc. & St. Andrew, Jamaica Bark (HD) α-Pinene (34.8%), β-pinene Insecticidal (Cylas Junor et al. (2008b)
Rendle (10.6%), terpinolene formicarius elegantulus,
(13.4%), α-terpineol (8.9%) LD50 = 11 μg g−1 insect)
St. Andrew, Jamaica Leaf (HD) α-Pinene (49.8%), β-pinene Insecticidal (Cylas Junor et al. (2008b)
(11.0%), α-terpineol (5.7%) formicarius elegantulus,
LD50 = 59 μg g−1 insect)
B. lancifolia (Schltdl.) Guerrero, Mexico Bark (SD) Limonene (14.5%), Anti-inflammatory (mouse Zúñiga et al. (2005)
Engl. terpinen-4-ol (7.3%), ear edema, 16.6% inhibition
α-terpineol (15.2%) elemol with a dose of 2.5 μg ear−1)
(6.0%), agarospirol (6.1%),
β-eudesmol (14.4%),
3,8-dimethylundecane
(5.6%), docosane (18.0%)
B. lunanii (Spreng.) St. Andrew, Jamaica Bark (HD) α-Pinene (51.2%), – Junor et al. (2010a)
C.D. Adams & Dandy ex α-terpineol (6.5%)
Proctor
St. Andrew, Jamaica Fruit (HD) trans-Pinocarveol (7.0%), – Junor et al. (2010a)
trans-verbenol (13.6%),
myrtenal (8.7%), verbenone
(14.7%), caryophyllene
oxide (5.6%)
Trelawny, Jamaica Fruit (HD) – Antibacterial, disk diffusion Junor et al. (2007)
assay (Escherichia coli,
Staphylococcus aureus,
MRSA)
St. Andrew, Jamaica Leaf (HD) α-Pinene (42.7%), – Junor et al. (2010a)
β-caryophyllene (14.2%),
caryophyllene oxide (12.2%)
A. DeCarlo et al.
B. microphylla A. Gray South Mountain Oleoresin (HD) δ-3-Carene (0.1–8.3%), – Tucker et al. (2009)
Park, Arizona myrcene (0.4–14.4%),
β-caryophyllene (35.7–
72.9%), caryophyllene oxide
(4.8–8.8%)
B. mirandae C.A. Toledo Tucson, Arizona Leaf (DCM extract) α-Pinene (6.6%), – Noge and Becerra
(greenhouse) α-phellandrene (15.0%), (2009)
β-caryophyllene (14.4%),
germacrene D (36.6%)
B. morelensis Ramirez Puebla, Mexico Bark (SD) α-Thujene (14.9%), – Zúñiga et al. (2005)
limonene (56.5%), terpinen-
4-ol (8.4%), α-terpineol
(7.2%), carvotanacetone
4 The Essential Oils of the Burseraceae
(6.5%)
Cañada ragion, Aerial parts (HD) α-Pinene (8.3%), Anti-inflammatory (rat paw Carrera-Martinez
Teotitlán de Flores α-phellandrene (51.9%), edema, 86.8% inhibition et al. (2014)
Magón, Oaxaca, p-cymene (5.0%), with dose of 0.5 mg kg−1)
Mexico β-phellandrene (10.8%),
β-caryophyllene (5.6%)
San Rafael, Stems and bark α-Pinene (5.8%), Antibacterial, broth Canales-Martinez
Coxcatlan, Mexico (HD) α-phellandrene (32.7%), microdilution assay et al. (2017)
o-cymene (8.7%), (Streptococcus pneumoniae,
β-phellandrene (14.8%), MIC 125 μg mL−1; Vibrio
β-caryophyllene (7.5%) cholerae, MIC
125 μg mL−1; Escherichia
coli, MIC 125 μg mL−1)
B. rupicola León de la Tucson, Arizona Leaf (DCM extract) α-Thujene (5.3%), α-pinene – Noge and Becerra
Luz (greenhouse) (10.3%), β-pinene (21.9%), (2009)
β-caryophyllene (18.3%),
germacrene D (31.9%)
(continued)
67
Table 4.1 (continued)
68
Bursera speciesa Collection site Essential oil Major components (>5%) Bioactivity of essential oil Reference
B. serrata Wall. ex Colebr. Lucknow, India Leaf (HD) Linalool (53.4%), α-terpineol – Sharma et al. (1996)
(26.4%)
B. simaruba (L.) Sarg. Monteverde, Costa Bark (HD) α-Thujene (11.9%), – Setzer (2014)
Rica α-phellandrene (29.1%),
o-cymene (13.1%),
β-caryophyllene (19.3%)
St. Andrew, Jamaica Bark (HD) α-Pinene (32.1%), β-pinene – Junor et al. (2008a,
(13.5%), p-mentha-1(7),8- b)
diene (5.6%), viridiflorol
(7.1%)
Costa Rica Fruit (HD) α-Terpinene (26.2%), – Rosales-Ovares and
δ-terpinene (20.4%), Cicció-Alberti
α-pinene (18.2%), p-cymene (2002)
(15.9%)
Trelawny, Jamaica Fruit (HD) – Antibacterial, disk diffusion Junor et al. (2007)
assay (Staphylococcus
aureus, MRSA)
St. Andrew, Jamaica Fruit (HD) α-Pinene (27.6%), sabinene – Junor et al. (2008a)
(8.1%), β-pinene (24.1%),
terpinen-4-ol (13.3%)
Monteverde, Costa Leaf (HD) α-Phellandrene (6.3%), – Setzer (2014)
Rica o-cymene (65.2%),
germacrene D (5.3%)
St. Andrew, Jamaica Leaf (HD) α-Pinene (10.2%), myrcene – Junor et al. (2008a)
(5.2%), β-elemene (5.6%),
β-caryophyllene (9.0%),
γ-muurolene (6.2%),
trans-cadina-1(6),4-diene
(9.7%)
A. DeCarlo et al.
Fouillole, Pointe-à- Leaf (HD) Limonene (46.7%), Cytotoxic (A-549 human Sylvestre et al.
Pître, Guadeloupe β-caryophyllene (14.7%), adenocarcinomic alveolar (2007)
α-humulene (13.3%), basal epithelial cells, IC50
germacrene D (7.6%) 42 μg mL−1; DLD-1 human
colon adenocarcinoma cells,
IC50 48 μg mL−1).
α-Humulene is the active
agent (IC50 62 μM and
71 μM on A-549 and
DLD-1 cells, respectively)
Trelawny, Jamaica Stem (HD) – Antibacterial, disk diffusion Junor et al. (2007)
assay (Staphylococcus
aureus, MRSA)
4 The Essential Oils of the Burseraceae
B. submoniliformis Engl. Puebla, Mexico Bark (SD) cis-p-Menth-2-en-1-ol – Zúñiga et al. (2005)
(6.9%), cuminol (5.4%),
docosane (8.0%)
B. tomentosa (Jacq.) Cabudare, Lara, Bark (HD) Nonane (6.4%), (Z)-β- – Moreno et al.
Triana & Planch. Venezuela ocimene (7.3%), (2010b)
bicyclogermacrene (6.6%),
spathulenol (11.4%),
globulol (8.9%), τ-cadinol
(8.8%)
Cabudare, Lara, Fruit (HD) Nonane (28.2%), (Z)-β- Antibacterial, disk diffusion Moreno et al.
Venezuela ocimene (47.6%), undecane assay (Staphylococcus (2010a)
(5.5%), germacrene D aureus, MIC 80 μg mL−1;
(11.1%) Enterococcus faecalis, MIC
120 μg mL−1;
Staphylococcus typhi, MIC
100 μg mL−1)
(continued)
69
Table 4.1 (continued)
70
Bursera speciesa Collection site Essential oil Major components (>5%) Bioactivity of essential oil Reference
B. velutina Bullock Guerrero, Mexico Bark (SD) Limonene (15.7%), – Zúñiga et al. (2005)
α-terpineol (10.7%),
trans-carveol (5.5%),
carvone (5.7%), spathulenol
(12.5%), β-eudesmol
(12.9%)
Altamirano, Leaf (DCM extract) α-Phellandrene (28.8%), – Noge et al. (2011)
Guerrero, Mexico β-phellandrene (11.0%),
2-phenylethanol (29.5%),
phytol (5.3%)
a
There is apparently some confusion regarding synonymous Bursera taxa (B. aloexylon, B. linanoe, B. delpechiana, B. citronella); the synonyms reported in
this table are based on those reported by the Missouri Botanical Garden (Missouri Botanical Garden 2017)
A. DeCarlo et al.
4 The Essential Oils of the Burseraceae 71
Bursera simaruba (L.) Sarg., “gumbo limbo,” “indio desnudo,” ranges from
south Florida, the Florida Keys, and the Bahamas throughout the West Indies and
Central America from southern Mexico to Colombia (Morton 1981). The bark,
gum, and leaves of this tree are used as traditional medicines throughout its range
(Morton 1981; Duke et al. 2009). For example, the plant is used in Belize to treat
wounds, insect bites, and skin sores (Arvigo and Balick 1993), leaves are used as a
bath by the Yucatec Maya to treat fever (Ankli et al. 1999), a leaf decoction is taken
in Cuba as a carminative (Beyra et al. 2004), and the leaf decoction is used in the
Bahamas to treat poisonwood (Metopium toxiferum) dermatitis (Higgs 1978). The
tree is commonly used as living fence posts in Costa Rica (Budowski and Russo
1993). There is wide variation in the essential oils of B. simaruba, depending not
only on the plant tissue but also the geographical origin of the plant (see Table 4.1).
Thus, for example, the leaf essential oil from Monteverde, Costa Rica, was
dominated by o-cymene (65.2%) (Setzer 2014), but the leaf oil from Fouillole,
Pointe-à-Pître, Guadeloupe, was rich in limonene (46.7%) (Sylvestre et al. 2007).
The leaf oil from St. Andrew, Jamaica, on the other hand, had α-pinene (10.2%),
β-caryophyllene (9.0%), and trans-cadina-1(6),4-diene (9.7%), as major components
(Junor et al. 2008a, b). Similarly, the bark essential oil from Costa Rica had abundant
α-phellandrene (29.1%) and β-caryophyllene (19.3%) (Setzer 2014), while the bark
oil from Jamaica showed α- and β-pinenes as major components (32.1% and 13.5%,
respectively) (Junor et al. 2008a, b). Clearly the geographical location plays a role
in the chemistry of these essential oils and must affect the biological activities and
likely affects the traditional uses as well.
Bursera tomentosa (Jacq.) Triana & Planch., “bálsamo de incienso,” is native to
the Lesser Antilles, Central America, northern Venezuela, and Colombia (Morton
1981; Moreno et al. 2010a, b). In northern South America, a decoction of the bark
is used to treat sciatica, pulmonary complaints, asthma, epilepsy, and venereal
diseases; the oleoresin is applied to ulcers and wounds (Morton 1981).
trees from infection, herbivory, and insect attack, but they have been prominent
aspects of human ethnobotanical medicine and religious practice throughout their
ranges for millennia (Langenheim 2003; Pichersky and Raguso 2018). Frankincense
features prominently in Indian Ayurvedic medicine and Chinese traditional medi-
cine, as well as having been used locally for oral hygiene, dressing wounds, calm-
ing/psychoactive effects, and anti-inflammatory treatment, among a variety of other
uses (Getahon 1976; Michie and Cooper 1991; Thulin 1999; Burkill 2000; Mies
et al. 2000; Frawley and Lad 2001; Dannaway 2010; Price et al. 2016; Acıduman
et al. 2017).
In modern times the resins and essential oils are still used in religious ceremonies
and are appreciated for their antiseptic, antimicrobial, anti-inflammatory, and psy-
choactive properties yielded by 300+ chemical constituents (Mertens et al. 2009;
Moussaieff and Mechoulam 2009). The resin is also of considerable modern interest
as the basis for cancer therapies (Roy et al. 2016). The most extensive studies on
components of frankincense have focused on the boswellic acids contained in the
resin. These boswellic acids have been shown to have effective anti-inflammatory
properties. Similar studies have shown the components of frankincense resin to
have a sedative activity, significantly reduce inflammation markers, induce apopto-
sis in human leukemia and prostate cells, and improve arthritis and many pharma-
cological therapies (Moussaieff and Mechoulam 2009). Recent studies have
provided scientific justification regarding the analgesic effects of frankincense
extracts and essential oils. This research concludes that frankincense is as effective
as the commercially available painkillers when comparing their analgesic properties
and bioactivity (Al-Harrasi et al. 2014). The essential oil compositions and biologi-
cal activities of each Boswellia species are summarized in Table 4.2.
Resins excreted from the wounds of the Boswellia species, often called oliba-
num, incense, or perhaps most commonly known as frankincense, have been traded
in the Arabic and African regions for more than 5000 years (Sultana et al. 2013;
Pickenhagen 2017). Frankincense has been used in numerous religious and cultural
ceremonies throughout the documented trade history of this traditionally important
commodity, and these uses of frankincense continue today. Frankincense was
burned in Assyria and Egypt as early as 3000 BC (Pickenhagen 2017). Chemical
analyses of archaeological samples taken from Egyptian tombs confirm the identity
of frankincense (Archier and Vieillescazes 2000; Mathe et al. 2004); between
2500 BC and 600 BC, frankincense was a major object of trade between Egypt and
the mysterious Land of Punt, likely located in the Horn of Africa (Kitchen 1971;
Phillips 1997). Following the decline of Punt, the center of trade shifted to Arabian
trade routes (B. sacra) and the Axumite Empire (B. papyrifera) (Butzer 1981; Hull
2008). Frankincense was considered the “scent of the gods” and was widely used in
wealthy households (Pickenhagen 2017). The same was true of Greece and Rome,
where it became hugely popular (Groom 1981). Frankincense also appears as a
component of medical and religious practice throughout the Middle East and in
Chinese medicine.
Boswellia sacra Flueck. is native to southern Oman, Yemen, Somaliland, and
Somalia (Thulin and Warfa 1987). The African populations are frequently referred
Table 4.2 Chemical compositions and biological activities of Boswellia essential oils
74
Boswellia
species Collection site Essential oil Major components (> 5%) Bioactivity of essential oil Reference
B. ameero Soqotra Island, Oleoresin α-Campholenal (13.4%), cosmene Inhibitor of acetylcholinesterase (IC50 Ali et al.
Balf. f. Yemen (HD) (34.9%), 3,4-dimethylstyrene 217 μg mL−1) (2008)
(17.3%), α-terpineol (12.4%),
1-(2,4-dimethylphenyl)ethanol
(20.3%)
Soqotra Island, Oleoresin α-Thujene (37.5%), α-pinene – Maděra et al.
Yemen (MeOH (20.5%), sabinene (6.8%), p-cymene (2017)
extract) (6.0%)
Soqotra Island, Oleoresin α-Thujene (26.4%), α-pinene (7.2%), – Maděra et al.
Yemen (MeOH terpinen-4-ol (26.1%), (E)-β- (2017)
extract) farnesene (6.1%)
Soqotra Island, Oleoresin α-Thujene (41.6%), terpinen-4-ol – Maděra et al.
Yemen (MeOH (14.1%), (E)-β-farnesene (8.9%), (2017)
extract) cembrene (9.2%)
Soqotra Island, Oleoresin α-Thujene (72.1%), sabinene (6.1%), – Maděra et al.
Yemen (MeOH (E)-β-farnesene (5.0%), cembrene (2017)
extract) (5.2%)
B. bullata Soqotra Island, Oleoresin β-Caryophyllene (7.2%), (E)-β- – Maděra et al.
Thulin Yemen (MeOH farnesene (7.5%), δ-cadinene (8.6%), (2017)
extract) guaiol (8.4%), α-cadinol (6.9%)
B. carteri Banaras Hindu Bark (HD) δ-3-Carene (5.6%), 2-phenylethanol Antifungal (Aspergillus niger, IC50 Prakash et al.
Birdw. University, Varanasi, (12.3%), benzyl acetate (13.4%), 616 μg mL−1; Alternaria alternata, IC50 (2014)
India limonene (9.5%), citronellol (8.0%) 354 μg mL−1; Cladosporium cladosporioides,
IC50 848 μg mL−1; Curvularia lunata,
IC50 < 250 μg mL−1; Fusarium oxysporum,
IC50 < 250 μg mL−1)
A. DeCarlo et al.
Commercial (drug Oleoresin Octanol (12.7%), octyl acetate Antibacterial, disk diffusion assay Abdel Wahab
market in Cairo, (HD) (60.0%)a (Staphylococcus aureus, Sarcina lutea, et al. (1987)
Egypt; from Mycobacterium phlei)
Somalia)
Not reported Oleoresin – Spasmogenic effect on guinea pig ileum Lis-Balchin
(HD) smooth muscle and Hart
(1997)
Commercial Oleoresin – Weakly antimicrobial (Staphylococcus aureus, Hammer et al.
(Sunspirit Oils Pty (HD) MIC 5000 μg mL−1; Candida albicans, MIC (1999)
Ltd., Byron Bay, 5000 μg mL−1)
NSW, Australia)
Ethiopia Oleoresin α-Pinene, 1,8-cineole, (E)-β-ocimene, – Basar et al.
(HD) octanol, linalool, octyl acetate, (2001)
4 The Essential Oils of the Burseraceae
(continued)
Table 4.2 (continued)
76
Boswellia
species Collection site Essential oil Major components (> 5%) Bioactivity of essential oil Reference
Commercial Oleoresin – Nematicidal (Bursaphelenchus xylophilus, LC50 Choi et al.
(Kyungdong (HD) 2100 μg mL−1) (2007)
medical market,
Seoul, Korea)
Commercial (White Oleoresin α-Thujene (7.3%), α-pinene (15.1%), Antibacterial, broth dilution assay Camarda et al.
Lotus Aromatics (HD) myrcene (8.2%), p-cymene (6.2%), (Staphylococcus aureus, MIC 3.52– (2007)
Ltd., Port Angeles, limonene (18.2%), α-cedrene (6.1%) 54.6 μg mL−1; Staphylococcus epidermidis,
WA, USA; from MIC 17.6 μg mL−1; Escherichia coli, MIC
Somalia) 54.6 μg mL−1; Pseudomonas aeruginosa, MIC
6.6 μg mL−1); antifungal (Candida albicans,
MIC 6.16 μg mL−1; Candida tropicalis, MIC
6.16 μg mL−1)b
Commercial Oleoresin α-Pinene (17.0%), myrcene (6.8%), Antibacterial, broth dilution assay Van Vuuren
(“various herbal (HD) p-cymene (6.2%), limonene (14.9%), (Staphylococcus aureus, MIC 5000 μg mL−1; et al. (2010)
shops”) β-caryophyllene (7.0%) Bacillus cereus, MIC 4000 μg mL−1;
Escherichia coli, MIC 8000 μg mL−1; Proteus
vulgaris, MIC 3000 μg mL−1; Candida
albicans, MIC 12000 μg mL−1)
Commercial Oleoresin α-Thujene (8.3%), α-pinene (22.8%), Antibacterial, broth dilution assay Van Vuuren
(“various herbal (HD) myrcene (5.5%), limonene (14.9%), (Staphylococcus aureus, MIC 8000 μg mL−1; et al. (2010)
shops”) β-caryophyllene (8.0%) Bacillus cereus, MIC 1500 μg mL−1;
Escherichia coli, MIC 8000 μg mL−1; Proteus
vulgaris, MIC 12800 μg mL−1; Candida
albicans, MIC 8000 μg mL−1)
A. DeCarlo et al.
Commercial Oleoresin α-Pinene (30.1%), myrcene (6.0%), Antibacterial, broth dilution assay Van Vuuren
(“various herbal (HD) limonene (20.4%), β-caryophyllene (Staphylococcus aureus, MIC 8000 μg mL−1; et al. (2010)
shops”) (6.9%) Bacillus cereus, MIC 4000 μg mL−1;
Escherichia coli, MIC 8000 μg mL−1; Proteus
vulgaris, MIC 3000 μg mL−1; Candida
albicans, MIC 6600 μg mL−1)
Commercial Oleoresin α-Thujene (7.9%), α-pinene (27.6%), Antibacterial, broth dilution assay Van Vuuren
(“various herbal (HD) myrcene (6.8%), limonene (14.6%), (Staphylococcus aureus, MIC 6000 μg mL−1; et al. (2010)
shops”) β-caryophyllene (5.6%) Bacillus cereus, MIC 2000 μg mL−1;
Escherichia coli, MIC 12000 μg mL−1; Proteus
vulgaris, MIC 3000 μg mL−1; Candida
albicans, MIC 8000 μg mL−1)
Commercial Oleoresin α-Thujene (7.7%), α-pinene (40.4%), Antibacterial, broth dilution assay Van Vuuren
4 The Essential Oils of the Burseraceae
(“various herbal (HD) myrcene (8.8%), p-cymene (5.3%), (Staphylococcus aureus, MIC 6000 μg mL−1; et al. (2010)
shops”) limonene (15.8%) Bacillus cereus, MIC 3000 μg mL−1;
Escherichia coli, MIC 6000 μg mL−1; Proteus
vulgaris, MIC 3000 μg mL−1; Candida
albicans, MIC 8000 μg mL−1)
Commercial Oleoresin α-Thujene (52.4%), sabinene (5.6%) Antibacterial, broth dilution assay Van Vuuren
(“various herbal (HD) (Staphylococcus aureus, MIC 16000 μg mL−1; et al. (2010)
shops”) Bacillus cereus, MIC 4000 μg mL−1;
Escherichia coli, MIC 6000 μg mL−1; Proteus
vulgaris, MIC 3000 μg mL−1; Candida
albicans, MIC 8000 μg mL−1)
Commercial Oleoresin α-Pinene (22.3%), limonene (11.9%), Antibacterial, broth dilution assay Van Vuuren
(“various herbal (HD) β-caryophyllene (7.8%) (Staphylococcus aureus, MIC 10400 μg mL−1; et al. (2010)
shops”) Bacillus cereus, MIC 4000 μg mL−1;
Escherichia coli, MIC 6000 μg mL−1; Proteus
vulgaris, MIC 3000 μg mL−1; Candida
albicans, MIC 5300 μg mL−1)
(continued)
77
Table 4.2 (continued)
78
Boswellia
species Collection site Essential oil Major components (> 5%) Bioactivity of essential oil Reference
Commercial Oleoresin α-Thujene (12.0%), α-pinene Antibacterial, broth dilution assay Van Vuuren
(“various herbal (HD) (23.6%), limonene (18.3%), (Staphylococcus aureus, MIC 8000 μg mL−1; et al. (2010)
shops”) β-caryophyllene (6.3%) Bacillus cereus, MIC 8300 μg mL−1;
Escherichia coli, MIC 4000 μg mL−1; Proteus
vulgaris, MIC 3000 μg mL−1; Candida
albicans, MIC 8000 μg mL−1)
B. carteri Commercial Oleoresin α-Thujene (5.3%), α-pinene (12.0%), Antibacterial, broth dilution assay Van Vuuren
Birdw. (“various herbal (HD) myrcene (9.9%), limonene (12.7%), (Staphylococcus aureus, MIC 10400 μg mL−1; et al. (2010)
shops”) β-caryophyllene (10.5%) Bacillus cereus, MIC 4000 μg mL−1;
Escherichia coli, MIC 6000 μg mL−1; Proteus
vulgaris, MIC 3000 μg mL−1; Candida
albicans, MIC 5300 μg mL−1)
Commercial Oleoresin α-Terpinene (7.9%), p-cymene – Yang et al.
(Skinmate, Puchon, (HD) (11.8%)c, γ-terpinene (16.9%), (2010)
Gyeonggido, Korea) p-menth-2-en-1-ol (34.5%)
Ethiopia Oleoresin Octyl acetate (34.7%), Cytotoxic (MCF-7, IC50 40.7 μg mL−1; Chen et al.
(HD) nardoeudesmol C (5.6%), nerolidol HepG2, IC50 57.0 μg mL−1; HeLa, IC50 (2013)
isobutyrate (18.3%) 55.5 μg mL−1; HS-1, IC50 39.7 μg mL−1; A459,
IC50 60.3 μg mL−1)
Commercial (Young Oleoresin – Cytotoxic (J82 human bladder tumor cells, IC50 Dozmorov
Living Essential (HD) 910 μg mL−1) et al. (2014)
Oils; from Somalia)
Commercial (herbal Oleoresin α-Pinene (23.0%), limonene (10.9%), Weak anticandidal activity (Candida albicans, Nikolić et al.
pharmacy, (HD) β-caryophyllene (8.3%), δ-cadinene MIC 1250 μg mL−1) (2016)
Rotterdam, (5.8%), caryophyllene oxide (11.1%)
Netherlands)
A. DeCarlo et al.
Commercial Oleoresin α-Thujene (12.0%), α-pinene Weak anticandidal activity (Candida albicans, Nikolić et al.
(Sensient Essential (HD) (31.8%), sabinene (5.4%), p-cymene MIC 2500 μg mL−1) (2016)
Oils, Bremen, (6.0%), limonene (17.9%),
Germany) β-caryophyllene (5.4%)
Commercial Oleoresin Dihydrocitronellyl acetate (55.6%), – Dounchaly
(Tehran market) (HD) α-santonin (9.0%) et al. (2016)
Commercial (Ameo, Oleoresin p-Cymene (10.0%), limonene – Setzer
Zija International) (HD) (22.4%), α-copaene (5.8%), (unpublished)
β-caryophyllene (22.2%), δ-cadinene
(9.4%)
Commercial (Scents Oleoresin α-Pinene (23.2%), limonene (22.4%), – Hamm et al.
of the Earth, Sun (headspace β-caryophyllene (6.9%) (2005)
City, USA; from SPME)
4 The Essential Oils of the Burseraceae
Somalia)
Commercial (Scents Oleoresin α-Pinene (6.3%), limonene (10.2%), – Hamm et al.
of the Earth, Sun (headspace β-caryophyllene (66.9%), α-humulene (2005)
City, USA; from SPME) (5.2%), 1(10,5-germacradien-4-ol
Aden) (5.7%), caryophyllene oxide (13.1%)
B. dalzielii Gombi, Adamawa Leaf (HD) α-Pinene (45.7%), γ-terpinene – Kubmarawa
Hutch. state, Nigeria (11.5%) et al. (2006)
Ségbana region, Leaf (HD) α-Pinene (15.2%), myrcene (5.7%), Enzyme inhibition (acetylcholinesterase, IC50 Kohoude et al.
Benin δ-3-carene (27.7%), p-cymene 67.1 μg mL−1; 5-lipoxygenase, IC50 (2017)
(9.5%), β-phellandrene (8.5%), 70.0 μg mL−1)
isolongifolene (6.2%)
B. Soqotra Island, Bark (HD) α-Thujene (9.3%), α-pinene (8.3%), Antibacterial, broth dilution assay Mothana et al.
dioscoridis Yemen camphor (5.5%), β-caryophyllene (Staphylococcus aureus, MIC 3620 μg mL−1; (2011)
Thulin (5.5%), caryophyllene oxide (5.0%) Bacillus subtilis, MIC 3620 μg mL−1)
Soqotra Island, Oleoresin α-Thujene (56.6%), α-pinene (8.3%), – Maděra et al.
Yemen (MeOH sabinene (10.0%), myrcene (6.0%), (2017)
extract) terpinen-4-ol (14.0%)
79
(continued)
Table 4.2 (continued)
80
Boswellia
species Collection site Essential oil Major components (> 5%) Bioactivity of essential oil Reference
Soqotra Island, Oleoresin α-Thujene (60.3%), terpinen-4-ol – Maděra et al.
Yemen (MeOH (26.5%) (2017)
extract)
Soqotra Island, Oleoresin α-Thujene (69.5%), α-pinene – Maděra et al.
Yemen (MeOH (10.5%), sabinene (7.4%), β-pinene (2017)
extract) (6.0%)
B. elongata Soqotra Island, Bark (HD) Verticilla-4(20),7,11-triene (8.2%), Antibacterial, broth dilution assay Mothana et al.
Balf. f. Yemen incensole (14.8%), incensole acetate (Staphylococcus aureus, MIC 4310 μg mL−1; (2011)
(7.4%) Bacillus subtilis, MIC 4310 μg mL−1)
Soqotra Island, Oleoresin β-Caryophyllene (39.1%), methyl – Ali et al.
Yemen (HD) cycloundecanecarboxylate (7.9%)d, (2008)
verticillol (52.4%)
Soqotra Island, Oleoresin α-Thujene (59.1%), (E)-β-farnesene – Maděra et al.
Yemen (MeOH (5.9%), cembrene (13.1%) (2017)
extract)
Soqotra Island, Oleoresin α-Pinene (34.3%), β-pinene (12.5%), – Maděra et al.
Yemen (MeOH p-cymene (6.4%), terpinen-4-ol (2017)
extract) (15.8%), (E)-β-farnesene (7.7%)
Soqotra Island, Oleoresin α-Thujene (49.2%), myrcene (9.4%), – Maděra et al.
Yemen (MeOH terpinen-4-ol (13.1%) (2017)
extract)
Soqotra Island, Oleoresin α-Thujene (16.2%), sabinene – Maděra et al.
Yemen (MeOH (16.1%), cembrene (28.5%) (2017)
extract)
B. frereana Commercial (Willy Oleoresin α-Thujene (8.1%), α-pinene (38.0%), – Basar (2005)
Birdw. Benecke GmbH, (HD) p-cymene (11.0%), β-caryophyllene
Hamburg, Germany) (5.3%)
A. DeCarlo et al.
Commercial Oleoresin α-Pinene (64.7%), sabinene (7.0%), Antibacterial, broth dilution assay Van Vuuren
(“various herbal (HD) p-cymene (5.4%) (Staphylococcus aureus, MIC 8000 μg mL−1; et al. (2010)
shops”) Bacillus cereus, MIC 3000 μg mL−1;
Escherichia coli, MIC 6000 μg mL−1; Proteus
vulgaris, MIC 3000 μg mL−1; Candida
albicans, MIC 6000 μg mL−1)
Commercial Oleoresin α-Thujene (19.9%), α-pinene Antibacterial, broth dilution assay Van Vuuren
(“various herbal (HD) (24.7%), p-cymene (12.3%), (Staphylococcus aureus, MIC 4000 μg mL−1; et al. (2010)
shops”) β-caryophyllene (5.3%) Bacillus cereus, MIC 1500 μg mL−1;
Escherichia coli, MIC 4000 μg mL−1; Proteus
vulgaris, MIC 3000 μg mL−1; Candida
albicans, MIC 6000 μg mL−1)
Commercial Oleoresin α-Thujene (33.1%), sabinene (5.1%), Antibacterial, broth dilution assay Van Vuuren
4 The Essential Oils of the Burseraceae
(“various herbal (HD) p-cymene (16.9%), β-caryophyllene (Staphylococcus aureus, MIC 12000 μg mL−1; et al. (2010)
shops”) (6.9%) Bacillus cereus, MIC 6600 μg mL−1;
Escherichia coli, MIC 4000 μg mL−1; Proteus
vulgaris, MIC 12800 μg mL−1; Candida
albicans, MIC 12000 μg mL−1)
Commercial (Scents Oleoresin α-Thujene (9.8%), α-pinene (12.4%), – (Hamm et al.
of the Earth, Sun (headspace p-cymene (7.8%) (2005)
City, USA; from SPME)
Somalia)
B. nana Soqotra Island, Oleoresin α-Thujene (57.6%), α-pinene (5.9%), – Maděra et al.
Hepper Yemen (MeOH sabinene (10.4%), β-pinene (10.2%) (2017)
extract)
B. neglecta Dibluk, Sidamo Oleoresin α-Pinene (16.7%), α-thujene (19.2%), – Başer et al.
S. Moore province, Ethiopia (HD) p-cymene (9.5%), terpinen-4-ol (2003)
(12.5%)
(continued)
81
Table 4.2 (continued)
82
Boswellia
species Collection site Essential oil Major components (> 5%) Bioactivity of essential oil Reference
Ethiopia Oleoresin α-Thujene (21.3%), α-pinene – Basar (2005)
(HD) (21.3%), p-cymene (11.8%),
terpinen-4-ol (5.3%)
Commercial Oleoresin α-Pinene (43.4%), β-pinene (13.1%), Antibacterial, broth dilution assay Van Vuuren
(“various herbal (HD) p-cymene (8.6%), terpinen-4-ol (Staphylococcus aureus, MIC 6000 μg mL−1; et al. (2010)
shops”) (12.5%) Bacillus cereus, MIC 2000 μg mL−1;
Escherichia coli, MIC 6000 μg mL−1; Proteus
vulgaris, MIC 3000 μg mL−1; Candida
albicans, MIC 6600 μg mL−1)
Borena region, Oleoresin – Antibacterial, broth dilution assay de Rapper
southern Ethiopia (HD) (Pseudomonas aeruginosa, MIC et al. (2012)
1300 μg mL−1); antifungal (Candida albicans,
MIC 1800 μg mL−1; Cryptococcus neoformans,
MIC 1300 μg mL−1)
Dubuluk, Borana Oleoresin α-Thujene (16.5%), α-pinene – Bekana et al.
zone, Oromiya (HD) (42.0%), terpinen-4-ol (28.2%) (2014)
region Ethiopia
Mega, Borana zone, Oleoresin α-Thujene (13.0%), α-pinene – Bekana et al.
Oromiya region, (HD) (32.6%), p-cymene (5.1%), terpinen- (2014)
Ethiopia 4-ol (29.9%)
Wachile, Borana Oleoresin α-Thujene (12.7%), α-pinene – Bekana et al.
zone, Oromiya (HD) (50.7%), terpinen-4-ol (17.5%), (2014)
region, Ethiopia verbenone (6.6%)
B. Metema, north Oleoresin Limonene (6.5%), octanol (8.0%), – Dekebo et al.
papyrifera Ethiopia (HD) octyl acetate (56.0%) (1999)
Hochst.
A. DeCarlo et al.
Commercial (White Oleoresin α-Pinene (5.2%), sabinene (6.0%), Antibacterial, broth dilution assay Camarda et al.
Lotus Aromatics (HD) octanol (17.8%), octyl acetate (Staphylococcus aureus, MIC 22.6– (2007)
Ltd., Port Angeles, (63.5%) 27.0 μg mL−1; Staphylococcus epidermidis,
WA, USA; from MIC 22.6 μg mL−1; Escherichia coli, MIC
Ethiopia) 27.0 μg mL−1; Pseudomonas aeruginosa, MIC
27.0 μg mL−1); antifungal (Candida albicans,
MIC 6.09 μg mL−1; Candida tropicalis, MIC
6.09 μg mL−1)b
Northern Ethiopia Oleoresin – Antibacterial, broth dilution assay de Rapper
(HD) (Staphylococcus aureus, MIC 1500 μg mL−1; et al. (2012)
Bacillus cereus, MIC 1600 μg mL−1;
Pseudomonas aeruginosa, MIC 1500 μg mL−1);
antifungal (Candida albicans, MIC
4 The Essential Oils of the Burseraceae
Boswellia
species Collection site Essential oil Major components (> 5%) Bioactivity of essential oil Reference
Commercial (Scents Oleoresin Octanol (13.9%), octyl acetate – Hamm et al.
of the Earth, Sun (headspace (64.6%), incensole acetate (10.8%) (2005)
City, USA; from SPME)
Ethiopia)
B. pirottae Gibe Valley, Oleoresin Terpinen-4-ol (14.6%), trans- – Başer et al.
Chiov. Ethiopia (HD) verbenol (15.5%) (2003)
B. Soqotra Island, Oleoresin α-Thujene (55.3%), p-cymene – Maděra et al.
popoviana Yemen (MeOH (9.0%), terpinen-4-ol (6.4%), (2017)
Hepper extract) cembrene (12.8%)
Soqotra Island, Oleoresin α-Thujene (29.9%), p-cymene – Maděra et al.
Yemen (MeOH (10.9%), terpinen-4-ol (8.0%) (2017)
extract)
Soqotra Island, Oleoresin α-Thujene (51.5%), p-cymene – Maděra et al.
Yemen (MeOH (9.0%), terpinen-4-ol (7.0%), (2017)
extract) (E)-β-farnesene (9.2%)
Soqotra Island, Oleoresin α-Thujene (67.3%), cembrene (6.3%) – Maděra et al.
Yemen (MeOH (2017)
extract)
B. rivae Bulagere, Ogaden Oleoresin α-Pinene (5.3%), δ-3-carene (9.6%), – Başer et al.
Engl. Region, Ethiopia (HD) limonene (14.8%), trans-verbenol (2003)
(6.8%)
Ethiopia Oleoresin α-Pinene (16.7%), δ-3-carene – Basar (2005)
(HD) (17.3%), limonene (21.1%)
A. DeCarlo et al.
Commercial (White Oleoresin α-Pinene (13.3%), δ-3-carene Antibacterial, broth dilution assay Camarda et al.
Lotus Aromatics (HD) (15.7%), p-cymene (7.1%), limonene (Staphylococcus aureus, MIC 13.3– (2007)
Ltd., Port Angeles, (28.0%), trans-verbenol (5.8%) 54.8 μg mL−1; Staphylococcus epidermidis,
WA, USA; from MIC 23.0 μg mL−1; Escherichia coli, MIC
Ethiopia) 3.53 μg mL−1; Pseudomonas aeruginosa, MIC
54.8 μg mL−1); antifungal (Candida albicans,
MIC 2.65 μg mL−1; Candida tropicalis, MIC
27.4 μg mL−1)b
Ogaden region, Oleoresin – Antibacterial, broth dilution assay de Rapper
southeastern (HD) (Pseudomonas aeruginosa, MIC et al. (2012)
Ethiopia 1000 μg mL−1); antifungal (Cryptococcus
neoformans, MIC 800 μg mL−1)
Filtu district, Oleoresin α-Pinene (37.3%), o-cymene (5.6%), – Bekana et al.
4 The Essential Oils of the Burseraceae
Boswellia
species Collection site Essential oil Major components (> 5%) Bioactivity of essential oil Reference
Commercial Oleoresin α-Pinene (22.5%), myrcene (5.5%), Antibacterial, broth dilution assay Van Vuuren
(“various herbal (HD) sabinene (6.9%), p-cymene (5.9%), (Staphylococcus aureus, MIC 4000 μg mL−1; et al. (2010)
shops”) limonene (11.2%), β-caryophyllene Bacillus cereus, MIC 3000 μg mL−1;
(7.6%) Escherichia coli, MIC 4000 μg mL−1; Proteus
vulgaris, MIC 3000 μg mL−1; Candida
albicans, MIC 8000 μg mL−1)
Commercial Oleoresin α-Thujene (11.2%), α-pinene Antibacterial, broth dilution assay Van Vuuren
(“various herbal (HD) (18.3%), limonene (13.1%), (Staphylococcus aureus, MIC 8000 μg mL−1; et al. (2010)
shops”) β-caryophyllene (7.2%) Bacillus cereus, MIC 2000 μg mL−1;
Escherichia coli, MIC 6000 μg mL−1; Proteus
vulgaris, MIC 3000 μg mL−1; Candida
albicans, MIC 8000 μg mL−1)
Hasik area, Oman Oleoresin α-Pinene (59.4–65.5%), myrcene Cytotoxic to breast tumor cells (MCF10-2A, Suhail et al.
(HD) (5.4–7.5%), limonene (8.4–9.0%) IC50 1470 μg mL−1; T47D IC50 690 μg mL−1; (2011)
MCF7, IC50 556 μg mL−1; MDA-MB-231 IC50
769 μg mL−1)
Hasik area, Oman Oleoresin α-Pinene (59.4–78.5%), myrcene Cytotoxic to pancreatic tumor cells (MIA Ni et al. (2012)
(HD) (2.6–5.4%), limonene (5.6–9.0%) MaCa-2, IC50 833 μg mL−1; Panc-28, IC50
813 μg mL−1; DANG, IC50 641 μg mL−1;
BxPC-3, IC50 741 μg mL−1)
B. sacra Dhofar region, “Hoojri” α-Pinene (76.0%) Antibacterial to Gram-positive organisms Al-Saidi et al.
Flueck. Oman oleoresin (Bacillus subtilis, Micrococcus luteus, (2012)
(HD) Staphylococcus aureus, MIC 5000 μg mL−1);
inactive to Gram-negative bacteria
Dhofar region, “Shaabi” α-Pinene (68.5%) Antibacterial to Gram-positive organisms Al-Saidi et al.
Oman oleoresin (Bacillus subtilis, Micrococcus luteus, (2012)
(HD) Staphylococcus aureus, MIC 5000 μg mL−1);
inactive to Gram-negative bacteria
A. DeCarlo et al.
Dhofar region, “Najdi” α-Pinene (46.8%), myrcene (8.9%), Antibacterial to Gram-positive organisms Al-Saidi et al.
Oman oleoresin limonene (15.9%) (Bacillus subtilis, Micrococcus luteus, (2012)
(HD) Staphylococcus aureus, MIC 5000 μg mL−1);
inactive to Gram-negative bacteria
Dhofar region, “Shathari” α-Pinene (64.7%), limonene (8.1%) Antibacterial to Gram-positive organisms Al-Saidi et al.
Oman oleoresin (Bacillus subtilis, Micrococcus luteus, (2012)
(HD) Staphylococcus aureus, MIC 5000 μg mL−1);
inactive to Gram-negative bacteria
Commercial Oleoresin α-Pinene (11.4%), myrcene (8.0%), – Niebler and
(J. Ertelt, (SAFE limonene (12.8%) Buettner
AureliaSan, distillation) (2015)
Bisingen, Germany;
from Oman)
4 The Essential Oils of the Burseraceae
Boswellia
species Collection site Essential oil Major components (> 5%) Bioactivity of essential oil Reference
Commercial Oleoresin α-Pinene (24.1%), p-cymene (7.0%), – Niebler and
(J. Ertelt, (SAFE limonene (11.3%) Buettner
AureliaSan, distillation) (2015)
Bisingen, Germany;
from Somalia)
Commercial Oleoresin α-Pinene (13.8%), myrcene (5.4%), – Niebler and
(C.E. Roeper, (SAFE p-cymene (9.7%), limonene (10.4%) Buettner
Hamburg, Germany; distillation) (2015)
from Somalia)
Salalah, Oman Oleoresin α-Pinene (61.6%) – Hakkim et al.
(hexane (2015)
extract)
Commercial Oleoresin – Rotundone (woody, coniferous) and mustakone Niebler et al.
(Premaveral Life (HD) (spicy, woody) identified as highly potent (2016)
GmbH, Mittelberg, odorants
Germany; from
Somalia)
Commercial (Scents Oleoresin α-Pinene (13.9%), myrcene (5.2%), – Hamm et al.
of the Earth, Sun (headspace p-cymene (5.5%), limonene (6.9%), (2005)
City, USA; from SPME)
Oman)
B. serrata Sokoto state, Bark (HD) α-Pinene (73.3%) – Kasali et al.
Roxb. ex Nigeria (2002)
Colebr.
Commercial (Willy Oleoresin α-Thujene (12.0%), α-pinene (8.0%), – Basar (2005)
Benecke GmbH, (HD) myrcene (38.0%), estragole (11.6%)
Hamburg, Germany)
A. DeCarlo et al.
Jabalpur area, Oleoresin α-Thujene (22.7%), tetrahydrolinalool – Singh et al.
Madhya Pradesh, (HD) (10.6%)e, benzyl tiglate (5.5%), (2007)
India epi-cubenol (5.2%), 10-epi-γ-
eudesmol (5.3%)
Commercial Oleoresin α-Thujene (47.4%), δ-3-carene – Singh et al.
(market in Amritsar, (HD) (9.6%), tetrahydrolinalool (7.0%)e, (2007)
Punjab India) epi-cubenol (9.1%)
Commercial Oleoresin α-Thujene (26.2%), δ-3-carene – Singh et al.
(market in Khari (HD) (7.9%), limonene (6.3%), (2007)
Baoli, New Delhi, tetrahydrolinalool (8.8%)e,
India) α-terpineol (5.8%)
Commercial Oleoresin α-Pinene (11.2%), α-thujene (29.5%), – Singh et al.
(market in Karol (HD) (2007)
4 The Essential Oils of the Burseraceae
Boswellia
species Collection site Essential oil Major components (> 5%) Bioactivity of essential oil Reference
Commercial Oleoresin α-Thujene (43.5%), α-pinene (7.2%), Antimicrobial, disk diffusion assay Sadhasivam
(Konark Herbals (HD) sabinene (7.8%), p-cymene (8.7%), (Propionibacterium acnes, Malassezia furfur, et al. (2016)
and Health Care) thujol (7.2%) Malassezia globosa, Trichophyton rubrum,
Trichophyton mentagrophytes)
Shivpuri forest area, Oleoresin α-Thujene (22.5%), α-pinene Antibacterial (Klebsiella pneumoniae, MIC Gupta et al.
Madhya Pradesh, (HD) (10.9%), myrcene (8.9%), α-terpineol 18.8 μg mL−1; Escherichia coli, MIC (2017)
India (7.8%), terpinyl isobutyrate (5.1%), 300 μg mL−1; Salmonella typhi MIC
eudesmol (11.5%) 37.5 μg mL−1; Streptococcus mutans, MIC
300 μg mL−1; Pseudomonas aeruginosa MIC
37.5 μg mL−1; Staphylococcus aureus, MIC
37.5 μg mL−1)
Commercial Oleoresin α-Thujene (61.4%), sabinene (5.5%) Antibacterial (Klebsiella pneumoniae, MIC Gupta et al.
(market, Mandsaur (HD) 150 μg mL−1; Escherichia coli, MIC (2017)
district, Madhya 150 μg mL−1; Salmonella typhi MIC
Pradesh, India 150 μg mL−1; Streptococcus mutans, MIC
150 μg mL−1; Pseudomonas aeruginosa MIC
37.5 μg mL−1; Staphylococcus aureus, MIC
37.5 μg mL−1)
Commercial Oleoresin α-Thujene (63.6%), α-pinene (5.5%), Antibacterial (Klebsiella pneumoniae, MIC Gupta et al.
(market, Mandsaur (HD) sabinene (5.9%) 37.5 μg mL−1; Escherichia coli, MIC (2017)
district, Madhya 300 μg mL−1; Salmonella typhi MIC
Pradesh, India 300 μg mL−1; Streptococcus mutans, MIC
300 μg mL−1; Pseudomonas aeruginosa MIC
75 μg mL−1; Staphylococcus aureus, MIC
75 μg mL−1)
A. DeCarlo et al.
B. serrata Commercial Oleoresin α-Thujene (65.6%), α-pinene (8.1%), Antibacterial (Klebsiella pneumoniae, MIC Gupta et al.
Roxb. ex (market, Neemuch (HD) sabinene (5.1%) 75 μg mL−1; Escherichia coli, MIC (2017)
Colebr. district, Madhya 300 μg mL−1; Salmonella typhi MIC
Pradesh, India) 150 μg mL−1; Streptococcus mutans, MIC
300 μg mL−1; Pseudomonas aeruginosa MIC
150 μg mL−1; Staphylococcus aureus, MIC
37.5 μg mL−1)
Commercial Oleoresin α-Thujene (69.8%) Antibacterial (Klebsiella pneumoniae, MIC Gupta et al.
(market, Neemuch (HD) 300 μg mL−1; Escherichia coli, MIC (2017)
district, Madhya 300 μg mL−1; Salmonella typhi MIC
Pradesh, India) 300 μg mL−1; Streptococcus mutans, MIC
300 μg mL−1; Pseudomonas aeruginosa MIC
300 μg mL−1; Staphylococcus aureus, MIC
4 The Essential Oils of the Burseraceae
300 μg mL−1)
Commercial (Scents Oleoresin α-Thujene (11.7%), myrcene (7.0%), – Hamm et al.
of the Earth, Sun (headspace kessane (8.0), incensole (6.9) (2005)
City, USA; from SPME)
India)
B. socotrana Soqotra Island, Oleoresin p-Cymene (7.1%), 2-thujen-4-ol Inhibitor of acetylcholinesterase (IC50 Ali et al.
Balf. f. Yemen (HD) (31.3%), (E)-2,3-epoxycarene 141 μg mL−1) (2008)
(51.8%)
Soqotra Island, Bark (HD) α-Thujene (7.6%), p-cymene Antibacterial, broth dilution assay Mothana et al.
Yemen (13.0%), camphor (11.6%), terpinen- (Staphylococcus aureus, MIC 1870 μg mL−1; (2011)
4-ol (6.1%), 2′-hydroxy-5′- Bacillus subtilis, MIC 1870 μg mL−1)
methoxyacetophenone (16.3%)
(continued)
91
Table 4.2 (continued)
92
Boswellia
species Collection site Essential oil Major components (> 5%) Bioactivity of essential oil Reference
Soqotra Island, Oleoresin α-Pinene (55.2%), myrcene (11.4%), – Maděra et al.
Yemen (MeOH limonene (8.0%), terpinen-4-ol (2017)
extract) (6.8%)
Soqotra Island, Oleoresin α-Thujene (46.0%), α-pinene (5.4%), – Maděra et al.
Yemen (MeOH myrcene (18.5%), p-cymene (7.1%), (2017)
extract) terpinen-4-ol (15.8%)
Soqotra Island, Oleoresin α-Pinene (92.4%) – Maděra et al.
Yemen (MeOH (2017)
extract)
B. thurifera Commercial Oleoresin α-Thujene (5.8%), α-pinene (41.2%), Antibacterial, agar diffusion assay (Beneckea Baratta et al.
Roxb. ex (Lothian Herbs, (HD) p-cymene (5.6%), limonene (16.7%), natriegens, Citrobacter freundii, Salmonella (1998)
Fleming Edinburgh, UK) trans-sabinene hydrate (10.8%) pullorum); antifungal (Aspergillus niger)
Commercial Oleoresin α-Pinene (28.0%), myrcene (5.6%), Antibacterial, broth dilution assay Van Vuuren
(“various herbal (HD) limonene (14.6%), β-caryophyllene (Staphylococcus aureus, MIC 10000 μg mL−1; et al. (2010)
shops”) (5.8%) Bacillus cereus, MIC 4000 μg mL−1;
Escherichia coli, MIC 6000 μg mL−1; Proteus
vulgaris, MIC 2000 μg mL−1; Candida
albicans, MIC 6000 μg mL−1)
a
The high concentrations of octanol and octyl acetate in this sample suggest that the plant may be B. papyrifera rather than B. carteri
b
It is likely that the MIC determinations have incorrect units, reported as μg mL−1 rather than mg mL−1 (see Schillaci et al. 2008), and that may account for the
large discrepancies in antimicrobial activities
c
This compound was reported as 1-ethyl-3,5-dimethylbenzene, but it is probably incorrect; 1-ethyl-3,5-dimethylbenzene is not a natural product and not listed
in the Dictionary of Natural Products (Dictionary of Natural Products 2017)
d
This component is likely incorrect; methyl cycloundecane carboxylate is not known to be a natural product and is not listed in the Dictionary of Natural
Products (Dictionary of Natural Products 2017)
e
This component is likely incorrect; dihydrolinalool is not known to be a natural product and is not listed in the Dictionary of Natural Products (Dictionary of
Natural Products 2017)
A. DeCarlo et al.
4 The Essential Oils of the Burseraceae 93
Historical uses of frankincense from a medicinal perspective are varied and numer-
ous. Some of the ailments treated by frankincense included coughing, vomiting,
gastrointestinal issues, ulcers, arthritis, and other various inflammatory diseases,
just to name a few of the traditional remedies of frankincense (Ammon 2008).
Oral Uses Frankincense resin has been chewed as a gum in many places. In the
Arab states, the resin of Boswellia frereana, endemic to Somalia/Somaliland, is
particularly prized. Somalis also chew the resin locally (Thulin 1999). In Soqotra,
the resin from several species is chewed locally for oral hygiene due to its antiseptic
qualities (Mies et al. 2000). In Egypt, fumigation with both frankincense and myrrh
was believed to treat toothaches (Acıduman et al. 2017). Boswellia papyrifera resin
is chewed in Ethiopia to quench thirst, while the bark is chewed to settle stomach
issues (Gebremedhin 1997; Gebrehiwot et al. 2003).
Treatment of Wounds Frankincense resin has been sprinkled over open wounds,
particularly those on the head, to prevent hemorrhaging (Price et al. 2016). Taken
4 The Essential Oils of the Burseraceae 95
with leek juice, it was thought to have a similar effect internally (Michie and Cooper
1991). It is also used in Kenya to dress wounds (Moussaieff and Mechoulam 2009).
Psychoactive Effects Sources from both the Middle East and Ethiopia mention the
resin’s calming effect, leading to it being used as a tranquilizer (Getahon 1976;
Epstein 1990). The psychoactive properties of the resin were well known:
frankincense was key to many religious ceremonies, likely acting as an entheogen
and mild narcotic (Dannaway 2010). Ibn Sina (Avicenna, Persia) mentions it as
being beneficial for amnesia and amentia (Hameed 1983). Indian Ayurvedic
medicine also acknowledges its impact on the nervous system (Frawley and Lad
2001).
Assorted Other Medicinal Uses Boswellia have been used for a variety of other
ailments as well. Boswellia dalzielii bark in West Africa is used for rheumatism,
septic sores, venereal diseases, and gastrointestinal issues (Burkill 2000; Evans
2009). Frankincense is used as a cancer/antitumoral therapy in Chinese traditional
medicine (Shen and Lou 2008), for leprosy (Tucker 1986), as well as to reduce fever
in Ethiopia (Fichtl and Addi 1994). In Egypt, frankincense was highly prized as a
fumigant and deodorizer. It was used in embalming bodies, as was its sister resin,
myrrh (Pickenhagen 2017).
The 19 species of Boswellia are broadly distributed across the lower elevation
(0–1500 m) frost-free tropics of Africa, Arabia, and the Indian subcontinent. Most
species inhabit arid environments, but a small number (B. dalzielii, B. serrata, B.
ovalifoliolata) require larger amounts of water and are found in the humid tropics;
however, many arid tropical specialists inhabit areas with significant levels of mist
or oceanic fog, which likely provides a large degree of their total water intake
(Somalia/Somaliland, Oman, Soqotra) (Thulin and Warfa 1987; Attorre et al. 2011;
Eslamieh 2017).
While many Boswellia are capable of growing on a variety of substrates, a subset
of the genus is specifically lithophilic, dwelling primarily on rocks and most often
cliffs. The most specialized cliff-dwellers are found in Soqotra (B. nana, B. bullatta,
B. dioscordis, B. popoviana). Soqotra represents a particularly interesting case, as at
least seven species of Boswellia have evolved in a relatively small area, four of
which specialize on cliffs and three of which are found in flat areas (Mies et al.
2000; Miller and Morris 2004). The reasons for this divergence in specialization are
96 A. DeCarlo et al.
not well understood. Other species, such as B. sacra and B. frereana in Somalia and
B. papyrifera in Ethiopia/Eritrea, may grow on either soil or on rock, flat, or cliff
areas (Thulin and Warfa 1987; Thulin 1999; Eshete et al. 2005). Boswellia sacra in
Somalia and B. frereana especially seem to specialize on jagged rocks, germinating
in small crevices filled with water and detritus and then forming swollen, disk-
shaped base to “sucker” themselves onto the rock (Thulin and Warfa 1987).
Boswellia trees are often reported as growing on limestone, while this may certainly
be true, in Somalia/Somaliland and Oman, they seem to prefer a layer of volcanic
rock overlying the limestone (DeCarlo, pers. obs.).
Members of the genus rarely exceed 10–12 m in height, even under ideal condi-
tions, although the growth form is highly dependent on the environment, and some
species occasionally reach up to 20 m (Thulin and Warfa 1987; Lemenih and Kassa
2011; Eslamieh 2017). Boswellia sacra, for instance, is highly variable morphologi-
cally throughout Somalia and Arabia; a Somali coastal population was observed to
have distinct trunks, swollen bases, barely undulating leaves, and panicle inflores-
cences. By contrast, a population in the interior featured a non-swollen base from
which the trees branched directly, distinctly undulating leaves and racemes. This
has led to multiple species being described, but the presence of varying intermedi-
ates between extremes has led to classification as a single species (Thulin and Warfa
1987). Boswellia also hybridize readily, leading to further confusion about species
distinctions (Eslamieh 2017).
Like the majority of the Burseraceae, Boswellia trees have resin canals in their
inner bark that contain a complex terpenoid gum-oleoresin known commercially as
frankincense. The gum-oleoresin protects the trees from both disease and boring
insects, which are a major cause of mortality (Langenheim 2003; Eshete 2011;
Tolera et al. 2013).
The trees are deciduous; some species flower without leaves, while others flower
while in leaf (Thulin and Warfa 1987; Mies et al. 2000; Lemenih and Kassa 2011).
The variation in floral coloration across species suggests possible variation in
pollination syndrome. However, this has been poorly studied. Boswellia papyrifera,
B. sacra, B. serrata, and B. ovalifoliolata appear to be pollinated primarily by bees
and to a lesser extent by wasps, ants, flies, and butterflies (Sunnichan et al. 2005;
Lippi et al. 2011; Raju et al. 2012). Boswellia ovalifoliolata may also be facultatively
ornithophilous, and B. ameero on Soqotra Island seems to specifically attract
sunbirds (Mies et al. 2000; Raju et al. 2012). Boswellia trees are anemochorous,
producing large sets of winged seeds. Fruits are non-fleshy, sometimes dehiscent
pods with three to five seeds (Thulin and Warfa 1987; Raju et al. 2012; Eslamieh
2017).
Many Boswellia populations that have been assessed in recent years show some
degree of threat and/or decline (Farah 2008; Attorre et al. 2011; Alaamri 2012;
Groenendijk et al. 2012). Declines are caused by a variety of factors, frequently
including uncontrolled ungulate grazing particularly by livestock such as goats
which kills seedlings and in several cases has completely blocked natural
regeneration of the trees (Attorre et al. 2011; Groenendijk et al. 2012). High levels
of tapping for resin deplete tree resources resulting in less reproductive effort;
4 The Essential Oils of the Burseraceae 97
fewer fruits, flowers, and seeds; reduced germination success; and overall reduction
in foliage production, annual carbon gain, and carbon stock (Rijkers et al. 2006;
Mengistu 2011; Eshete et al. 2012; Mengistu et al. 2012). Tapping also opens
wounds and depletes the amount of resin available to fight off microbial and
arthropod attack, possibly increasing mortality from natural enemies. Fire, cutting
for wood, and land clearing for other activities further increase adult mortality
(Groenendijk et al. 2012). Climate change may also pose a significant threat, though
this has not been well-investigated.
Due to the anthropogenic nature of the threats, cliff-dwelling Boswellia seem to
do better than species in flat areas (Attorre et al. 2011), with less grazing and a
higher level of regeneration. In species in which some individuals are located on
cliffs and others in flat areas, cliff-dwelling populations may act as genetic reserves,
with limited tapping and grazing, and higher regeneration (Attorre et al. 2011;
Eshete et al. 2012; DeCarlo, pers. obs.).
The genus Commiphora comprises between 150 and 200 species of resiniferous
shrubs or trees characterized by peeling bark, soft wood, small leaves, production of
aromatic gum-oleoresin, and a tendency toward pachycauly (Thulin 1999; Mahr
2012). Commiphora species are broadly distributed across tropical and subtropical
areas of sub-Saharan Africa, Madagascar, Arabia, across to Iran, Pakistan, and
India. A single species, C. leptophloeos, is found in southeastern Brazil (Mahr
2012). Commiphora produce aromatic gum-oleoresins, the most common of which
are known as myrrh and opopanax (Tucker 1986; Thulin and Claeson 1991). The
resin has been used religiously and medicinally throughout the ancient world
including ancient Greece, Egypt, China, and in the Middle East (Hanuš et al. 2005;
Pickenhagen 2017). The resin functions as protection for the trees by sealing wounds
and aiding in defense against invasive insects, disease, herbivory, and infection
(Langenheim 2003; Pichersky and Raguso 2018). It has been traditionally utilized
to treat gastrointestinal diseases, inflammatory disease, fractures, obesity, blood
stagnation, and as an analgesic (Shen et al. 2012). Today it is a valuable commodity
4 The Essential Oils of the Burseraceae 99
for its use in aromatherapy and as a perfume additive (Shen et al. 2012). The
essential oil compositions and biological activities of the Commiphora species are
summarized in Table 4.3.
Commiphora myrrha (T. Nees) Engl./Commiphora molmol (Engl.) Engl. ex
Tschirch predominantly grow in the dry forests of Africa, India, and the Arabian
Peninsula (Eslamieh 2016). The resin oil chemistry is variable but often contains
furanoeudesma-1,3-diene (940%), lindestrene (6–15%), curzerene (15–40%), and
sometimes 2-acetoxyfuranodiene (6–10%) (Başer et al. 2003; Morteza-Semnani
and Saeedi 2003; Marongiu et al. 2005; Hanuš et al. 2008; Nikolić et al. 2016). The
resin has traditionally been used for controlling inflammation and pain, as well as
treatment of blood stagnation, dermatological care, and treatment of trauma (Shen
and Lou 2008; Shen et al. 2012). The resin has been shown to have antimicrobial,
antiviral, anti-inflammatory, and analgesic effects (Hammer et al. 1999; Ali 2007;
Shen and Lou 2008; Shen et al. 2012; Adam and Selim 2013).
Commiphora mukul (Hook. ex Stocks) Engl. [syn. Commiphora wightii (Arn.)
Bhandari] grows predominantly in India and Pakistan (Eslamieh 2016). The oil
contains curzerene, furanoeudesma-1,3-diene, lindestrene, and curzerenone (Saeed
and Sabir 2004). It is predominantly used in Indian and Arabian traditional medi-
cine for its anti-inflammatory, anticoagulant, and antibacterial properties, as well
as for atherosclerosis (Sarup et al. 2015; Ur Rehman et al. 2017). It has also been
noted to treat bone fractures, arthritis, cardiovascular disease, and lipid disorders
(Shen et al. 2012).
Commiphora guidotii Chiov., a source of opopanax, is endemic to Ethiopia and
Somalia (Gebrehiwot et al. 2015) where it grows on rocky slopes between 70 and
800 m (Thulin and Claeson 1991). The essential oil contains α-santalene (15–20%),
(E)-β-ocimene (6–30%), and (Z)-α-bisabolene (20–30%) as major constituents
(Craveiro et al. 1983; Başer et al. 2003; Gebrehiwot et al. 2015; Yeo et al. 2016).
In Somalian traditional medicine, it is mostly used to treat stomach ailments,
wounds, and diarrhea (Shen et al. 2012). In Ethiopia, the resin is sometimes fed to
cattle to improve dairy production (Gebrehiwot et al. 2015).
Commiphora africana (A. Rich.) Engl. is widely distributed across sub-Saharan
Africa. The oil consists of bisabolone, β-sesquiphellandrene, curcumenes, and
α-oxobisabolene (Ayédoun et al. 1998; Avlessi et al. 2005). In many African
countries, the plant is used for cancer treatment, malaria, and inflammatory disease
(Compaoré et al. 2016). It is also commonly used in Nigeria for removing tapeworms
from the body and Uganda for treating wounds (Shen et al. 2012).
Commiphora exudates have been used for their therapeutic, religious, and medici-
nal values throughout the ancient world, including Rome, Greece, China, Babylon,
and India for at least 3000 years (Shen et al. 2012; Pickenhagen 2017). Commiphora
products are also used locally where they occur for a variety of medicinal purposes.
Myrrh was a highly valuable commodity in the ancient world, where it was used
Table 4.3 Chemical compositions and biological activities of Commiphora essential oils
100
Commiphora Essential
species Collection site oil Major components (>5%) Bioactivity of EO Reference
C. africana Abomey-Calavi, Leaf (HD) (Z)-γ-Bisabolene (10.0%), α-oxobisabolene – Ayédoun et al.
(A. Rich.) Engl. Benin (61.6%) (1998)
Bohicon, Benin Leaf (HD) ar-Curcumene (8.2%), γ-curcumene (6.7%), – Avlessi et al. (2005)
β-bisabolene (5.2%), β-sesquiphellandrene
(19.1%), (6S,7R)-bisabolone (38.4%)
C. erythraea Engl. Commercial Oleoresin 1(10),4-Furanodien-6-one (21.5%), – Marcotullio et al.
(Agarsu Liben (HD) 1,10(15)-furanogermacradien-6-one (14.3%), (2009)
Cooperative) 3R-methoxy-4S-furanogermacra-1E,10(15)-
dien-6-one (7.4%)
Commercial Oleoresin Camphene (8.2%), β-elemene (8.2%), Antifungal (Alternaria Fraternale et al.
(Agarsu Liben (HD) α-gurjunene (6.0%), 1(10),4-furanodien-6-one solani, MIC (2011)
Cooperative) (9.0%) 3000 μg mL−1; Fusarium
culmorum, MIC
5500 μg mL−1;
Phytophtora cryptogea,
MIC 5500 μg mL−1)
Commercial Oleoresin β-Elemene (5.4%), 1(10),4-furanodien-6-one – Fraternale et al.
(Agarsu Liben (SD) (20.6%), 1,10(15)-furanogermacradien-6-one (2011)
Cooperative) (10.4%)
C. gileadensis (L.) Makkah, Saudi Aerial parts Terpinen-4-ol (8.5%), δ-cadinene (5.0%), Cytotoxic (SK-Mel, IC50 Al-Massarany et al.
C. Chr. [syn. C. Arabia (HD) α-calacorene (9.4%) 97 μg mL−1; KB, IC50 (2007)
opobalsamum (L.) 70 μg mL−1; BT-549, IC50
Engl.] 48 μg mL−1; SK-OV3,
IC50 82 μg mL−1)
Ein Gedi Botanical Aerial parts α-Pinene (7.2%), sabinene (21.1%), Cytotoxic (BS-24-1 Amiel et al. (2012)
Garden, Israel (HD) β-caryophyllene (20.1%), germacrene D mouse lymphoma cells,
(19.6%), terpinen-4-ol (5.3%) MoFir human B
lymphocytes)
A. DeCarlo et al.
Almog, Dead Sea, Aerial parts α-Pinene (18.4%), sabinene (29.1%), β-pinene – Dudai et al. (2017)
Israel (HD) (8.2%), p-cymene (6.4%), γ-terpinene (5.9%),
terpinen-4-ol (6.9%)
Ein Gedi Botanical Fruit (HD) α-Pinene (15.9%), sabinene (35.9%), β-pinene – Dudai et al. (2017)
Garden, Israel (18.0%), p-cymene (5.3%), limonene (6.2%),
terpinen-4-ol (5.3%)
C. gileadensis (L.) Ein Gedi Botanical Leaf (HD) α-Pinene (11.2%), sabinene (15.8%), β-pinene – Dudai et al. (2017)
C. Chr. [syn. C. Garden, Israel (5.8%), p-cymene (8.4%), γ-terpinene (5.8%),
opobalsamum (L.) terpinen-4-ol (18.5%)
Engl.]
Ein Gedi Botanical Stem (HD) α-Pinene (15.4%), sabinene (28.5%), β-pinene – Dudai et al. (2017)
Garden, Israel (7.5%), γ-terpinene (8.1%), terpinen-4-ol
(11.6%)
4 The Essential Oils of the Burseraceae
C. guidottii Chiov. South-eastern Oleoresin α-Santalene (major), β-santalene, epi-β- – Craveiro et al.
Somalia (SD) santalene, β-bergamotene, (E)-β-farnesene, (1983)
α-bisabolene (major), β-bisabolene, furanodiene
(major) (percentages not reported)
Bulagere, Ogaden Oleoresin (E)-β-Ocimene (33.0%), α-santalene (15.8%), – Başer et al. (2003)
region, Ethiopia (HD) β-trans-bergamotene (6.6%), α-cis-bisabolene
(22.2%)
(continued)
101
Table 4.3 (continued)
102
Commiphora Essential
species Collection site oil Major components (>5%) Bioactivity of EO Reference
Ogaden region, Oleoresin (E)-β-Ocimene (6.7%), α-santalene (19.5%), Antibacterial (Bacillus Gebrehiwot et al.
eastern Ethiopia (SD) α-trans-bergamotene (9.3%), curcerene pubilus, MIC 50 μg mL−1; (2015)
(11.4%), furanoeudesma-1,3-diene (18.6%), Bacillus subtilis, MIC
isofuranodiene (6.8%) 100 μg mL−1; Escherichia
coli, MIC 25 μg mL−1;
Salmonella typhi, MIC
10 μg mL−1; Shigella
boydii, MIC 50 μg mL−1;
Shigella dysenteriae,
MIC 50 μg mL−1;
Shigella flexneri, MIC
50 μg mL−1; Shigella
soneii, MIC 50 μg mL−1;
Staphylococcus aureus,
MIC 25 μg mL−1; Vibrio
cholerae, MIC
10 μg mL−1). Antifungal
(Aspergillus niger, MIC
1000 μg mL−1; Candida
albicans, MIC
400 μg mL−1; Penicillium
funiculosum, MIC
1000 μg mL−1;
Penicillium notatum, MIC
1000 μg mL−1)
A. DeCarlo et al.
C. guidottii Chiov. Commercial Oleoresin (E)-β-Ocimene (11.5%), α-santalene (21.9%), β-Bisabolene cytotoxic Yeo et al. (2016)
(Sigma-Aldrich) (SD) trans-α-bergamotene (9.0%), (Z)-α-bisabolene (human breast tumor cell
(27%), β-bisabolene (5.1%) lines, MCF7, IC50
66.9 μg mL−1; MCF10A,
IC50 114 μg mL−1;
MDA-MB-231, IC50
98.4 μg mL−1; SKBR3,
IC50 70.6 μg mL−1;
BT474, IC50
74.3 μg mL−1);
cytotoxicity due to
induction of apoptosis
C. habessinica Hujarriyah district, Oleoresin β-Elemene (32.1%), α-selinene (18.9%), – Awadh Ali et al.
4 The Essential Oils of the Burseraceae
Commiphora Essential
species Collection site oil Major components (>5%) Bioactivity of EO Reference
C. leptophloeos Catimbau National Leaf (HD) α-Phellandrene (26.3%), β-phellandrene Oviposition deterrent Da Silva et al.
(Mart.) J.B. Gillett Park, Pernambuco (12.9%), β-caryophyllene (18.0%), α-humulene (Aedes aegypti, 59% (2015)
state, Brazil (5.5%), germacrene D (6.0%) reduction at 25 μg mL−1);
larvicidal (Aedes aegypti,
LC50 99.4 μg mL−1);
activity due to
β-caryophyllene and
α-humulene
C. molmol (Engl.) Shiraz, Fars Oleoresin β-Elemene (8.4%), curzerene (40.1%), – Morteza-Semnani
Engl. ex Tschirch province, Iran (HD) furanoeudesma-1,3-diene (15.0%), and Saeedi (2003)
(syn. C. myrrha var. acetoxyfuranodiene (6.5%)
molmol Engl.)
Commercial (market Oleoresin – Antifungal, disk diffusion Ali (2007)
in Baghdad, Iraq) (HD) assay (Aspergillus niger,
Aspergillus flavus)
C. molmol (Engl.) Commercial Oleoresin Limonene (12.3%), benzyl alcohol (5.6%), Larvicidal (Culex pipiens, Habeeb et al. (2009)
Engl. ex Tschirch (El-Captain (HD) carvone (21.1%) LC50 0.99 μL mL−1)
(syn. C. myrrha var. Company, Egypt
molmol Engl.)
Commercial (market Oleoresin – Antibacterial (Bacillus Adam and Selim
in Al Jouf, Saudi (HD) cereus, MIC (2013)
Arabia) 250 μg mL−1; Bacillus
subtilis, MIC
250 μg mL−1;
Staphylococcus aureus,
MIC 100 μg mL−1;
Escherichia coli, MIC
250 μg mL−1; Klebsiella
A. DeCarlo et al.
pneumoniae, MIC
50 μg mL−1)
Commercial (Tamar Oleoresin Isofuranogermacrene (6.7%), furanoeudesma- – Hanuš et al. (2008)
Ltd., Israel) (EtOH 1.3-diene (9.0%), 2-acetoxyfuranodiene (9.8%)
extract)
Commercial (Pamir Oleoresin Isofuranogermacrene (17.9%), furanoeudesma- – Hanuš et al. (2008)
Ltd., Israel) (EtOH 1.3-diene (20.6%), lindestrene (6.2%),
extract) 2-methoxyfuranodiene (7.3%),
2-acetoxyfuranodiene (8.8%)
C. myrrha (T. Nees) Commercial (Améo, Oleoresin α-Pinene (6.8%), neryl acetate (6.3%), – Setzer
Engl. Zija International) (HD) curzerene (16.1%), furanoeudesma-1,3-diene (unpublished)
(18.1%), lindestrene (6.9%)
Somaliland Oleoresin β-Elemene (20.2%), curzerene (23.7%), – Satyal
(HD) furanoeudesma-1,3-diene (24.6%), lindestrene (unpublished)
(6.7%)
4 The Essential Oils of the Burseraceae
Commiphora Essential
species Collection site oil Major components (>5%) Bioactivity of EO Reference
C. myrrha (T. Nees) Commercial (Harraz Oleoresin Analysis in doubt Antimicrobial (Bacillus Mohamed et al.
Engl. Herbs Co., Cairo, (HD) circulans, MIC (2014)
Egypt) 600 μg mL−1; Bacillus
subtilis, MIC
200 μg mL−1; Escherichia
coli, MIC 100 μg mL−1;
Pseudomonas
aeruginosa, MIC
200 μg mL−1;
Streptococcus faecalis,
MIC 100 μg mL−1;
Saccharomyces
cerevisiae, MIC
100 μg mL−1)
Commercial Oleoresin Curzerene (34.7%), furanoeudesma-1,3-diene Weakly antifungal Nikolić et al. (2016)
(Sensient Essential (HD) (32.8%), lindestrene (10.2%) (Candida albicans, MIC
Oils, Germany) 2500 μg mL−1)
C. ornifolia Soqotra Island, Bark (HD) endo-Fenchol (15.5%), camphor (27.3%), Antibacterial, broth Mothana et al.
J.B. Gillett Yemen caryophyllene oxide (6.5%), thunbergol (6.4%) dilution assay (Bacillus (2010)
subtilis, MIC
400 μg mL−1;
Staphylococcus aureus,
MIC 810 μg mL−1)
Soqotra Island, Oleoresin α-Thujene (14.5%), terpinen-4-ol (10.6%), – Maděra et al. (2017)
Yemen (MeOH β-caryophyllene (8.2%), α-humulene (24.8%)
extract)
C. parvifolia Engl. Soqotra Island, Bark (HD) Camphor (9.1%), caryophyllene oxide (14.2%), – Mothana et al.
Yemen β-eudesmol (7.7%), bulnesol (5.7%), palmitic (2010)
A. DeCarlo et al.
Treatment of Wounds Myrrh can be applied to wounds and lesions due to its anti-
septic properties (El Ashry et al. 2003; Walsh et al. 2010; Gebrehiwot et al. 2015).
In India, a paste of the resin is applied to cracks in the feet (Reddy et al. 2009).
Commiphora guidottii resin is applied topically to wounds in Somalia (Thulin and
Claeson 1991), while C. erythraea, C. kua, and C. habessinica are used by the
Borana people of southern Ethiopia for burns, wounds on cattle, and killing cattle
ticks (Gemedo-Dalle et al. 2005). Commiphora holtziana is likewise used for ecto-
parasites in Kenya (Birkett et al. 2008).
Antitumoral Uses Commiphora resin has long been used in Arabian traditional
medicine to treat tumors of the liver, stomach, breast, and head (Ageel et al. 1987;
El Ashry et al. 2003; Amin and Mousa 2007; Evans 2009).
congealed blood and promoting blood circulation (Shen et al. 2012). In India, the
leaves of C. caudata are used to reduce inflammation and pain (Annu et al. 2010),
while the resin is used throughout Eastern Africa and Arabia for inflammation and
rheumatism (Iwu 2014). The traditional uses of myrrh to treat inflammation are
supported by in vivo anti-inflammatory screening of myrrh resin extracts in mice
(Su et al. 2011).
Assorted Other Uses Commiphora resin and oil have been and are currently used
for a myriad of other uses. In India, it is used to alleviate pain from bone fractures,
cardiovascular disease, stomach aches, and the common cold (Shen et al. 2012). In
Iran, myrrh is also used to protect women who are in labor against infection
(Mahboubi and Kashani 2016). In the ancient cultures surrounding the Fertile
Crescent, myrrh was primarily used for ointment, perfumes, and the embalming of
Egyptian mummies (Northrup et al. 2005). Commiphora are also used to treat
diarrhea and stomach ailments in Somalia (Thulin and Claeson 1991) and by Bapedi
healers in South Africa (Semenya and Maroyi 2012).
The word Canarium is derived from the Malay word “Kanari.” There are 77 species
of the Canarium genus, mostly found in tropical Asia and the Pacific, but two
species are found in tropical Africa (Mabberley 2008). Members of the Canarium
genus represent medium to large trees up to 40–50 m in height or, rarely, shrubs
(Mogana and Wiart 2011), and they are important sources of timber, food, oils, and
traditional medicines (Thomson and Evans 2006).
Canarium schweinfurthii Engl. ranges in equatorial forest regions in tropical
Africa and is used in various traditional medicinal practices (Tcheghebe et al. 2016).
The leaves are used as stimulant, against malarial fever, postpartum pain, constipa-
tion, and diarrhea (Tcheghebe et al. 2016). Traditionally, the stem bark decoction is
Table 4.4 Chemical compositions and biological activities of Aucoumea klaineana essential oils
110
Aucoumea
species Collection site Essential oil Major components (>5%) Bioactivity of essential oil Reference
A. klaineana Libreville, Gabon Oleoresin (HD) α-Pinene (20.6%), α-phellandrene – Liang et al. (1988a)
Pierre (11.2%), p-cymene (30.2%),
limonene (5.4%), α-terpineol (5.2%)
Sebang Oleoresin (HD) δ-3-Carene (72.3%), terpinolene – Koudou et al. (2009)
Herbarium, (6.3%)
Libreville, Gabon
Lolodorf, Oleoresin (HD) α-Pinene (29.3%), α-phellandrene – Dongmo et al. (2010)
Cameroon (30.9%), p-cymene (9.2%),
1,8-cineole (9.0%)
Mekuê forest, Oleoresin (HD) – Antibacterial, disk diffusion assay Obame et al. (2014)
Mebane Endama, (Bacillus cereus, Escherichia coli,
Oyem, Gabon Staphylococcus aureus, MIC
10,000 μg mL−1)
Lolodorf, Oleoresin (HD) α-Pinene (29.3%), α-phellandrene Not antifungal (Aspergillus spp.) Ambindei et al.
Cameroon (30.9%), p-cymene (9.2%), (2014)
1,8-cineole (9.0%)
N’Toum, Estuaire Oleoresin α-Pinene (5.9%), δ-3-carene (8.6%), – Medzegue et al.
province, Gabon (headspace) α-phellandrene (63.4%), limonene (2013)
(6.1%), β-phellandrene (5.9%),
p-cymene (5.4%)
Cocobeach, Oleoresin α-Pinene (5.8%), δ-3-carene (40.1%), – Medzegue et al.
Estuaire province, (headspace) α-phellandrene (12.9%), limonene (2013)
Gabon (16.5%), p-cymene (10.3%)
Bokoué, Estuaire Oleoresin α-Pinene (5.3%), δ-3-carene (8.1%), – Medzegue et al.
province, Gabon (headspace) limonene (24.1%), p-cymene (2013)
(42.5%), α-terpineol (7.2%)
Bokoué, Estuaire Oleoresin α-Pinene (5.4%), δ-3-carene (12.8%), – Medzegue et al.
province, Gabon (headspace) α-phellandrene (19.6%), limonene (2013)
A. DeCarlo et al.
used as a remedy for roundworms, colic, stomach pains, postpartum pains, dysen-
tery, and gonorrhea (Tcheghebe et al. 2016). Sore throat is treated from a drink
made from burnt seed of C. schweinfurthii (Tcheghebe et al. 2016). Decoctions of
the tree bark are used in the Ivory Coast against cough, to treat chest pain in Sierra
Leone, venereal diseases in Cameroon, and remedies for abscesses and dysentery in
Nigeria (Dongmo et al. 2010). In the Congo and the Central African Republic, the
plant is used as a stimulant, emollient, and as a treatment for rheumatism (Bouquet
1969). Scientifically, extracts from the tree have demonstrated several biological
activities, including antimalarial, antineoplastic, antioxidant, antimicrobial, antidia-
betic, analgesic, nephroprotective, anthelmintic, and termiticidal activities
(Tcheghebe et al. 2016). The oleoresin produced by C. schweinfurthii has an odor
reminiscent of lavender, and it is used as incense in Uganda (Nagawa et al. 2015).
The essential oil (EO) from the oleoresin has shown significant analgesic effects in
mouse models of pain (acetic acid-induced writhing and hot plate test) (Koudou
et al. 2005). The resin oil was tested for anti-termitic activity against Macrotermes
bellicosus and was found to be remarkably active, and its major components were
also tested to confirm its anti-termitic property (Nagawa et al. 2015). The resin oil
has also shown antifungal activity against several Aspergillus species (Ambindei
et al. 2014).
A popular commercial essential oil, “elemi,” obtained from the oleoresin of
Canarium luzonicum (Blume) A. Gray (Villanueva et al. 1993), is used as an expec-
torant in addition to treatment of stomach disorders (Rajagopal 2014). Traditionally
the dried powdered oleoresin from Canarium strictum Roxb., known in India as
black dammer resin, is used to treat skin diseases, hernia, syphilis, asthma, rheuma-
tism, and fevers. The resin has shown anti-inflammatory activities (Ragunathan and
Senthamarai 2013). Canarium bengalenese Roxb. is locally called “tram hong” in
Vietnam, and its bark and leaves are used externally in rheumatic swellings (Thang
et al. 2004). The chemical compositions and biological activities of Canarium
essential oils are summarized in Table 4.5.
(7.5%)
C. schweinfurthii Boukoko, Central African Oleoresin n-Octanol (9.5%), octyl acetate (60.0%), Antinociceptive, mouse Koudou et al. (2005)
Engl. Republic (HD) (E)-nerolidol (14.0%) (acetic acid-induced
writhing, ED50
1.6 mL kg−1; hot plate
ED50 1.4 mL kg−1)
C. schweinfurthii Boukoko, Central African Oleoresin – Antibacterial, broth Obame et al. (2007b)
Engl. Republic (HD) dilution assay (Listeria
innocua,
Staphylococcus aureus,
Staphylococcus
camorum, MIC
2500 μg mL−1),
antifungal (Candida
albicans, MIC
4 The Essential Oils of the Burseraceae
2500 μg mL−1)
Lolodorf, Cameroon Oleoresin p-Cymene (9.8%), limonene (42.7%), Inhibitor of Dongmo et al. (2010)
(HD) α-terpineol (34.4%) 5-lipoxygenase (IC50
62.6 μg mL−1)
Mbouda, Cameroon Oleoresin p-Cymene (9.8%), limonene (42.7%), – Dongmo et al. (2010)
(HD) α-terpineol (34.4%)
Côte d’Ivoire Root (HD) δ-2-Carene (14.5%), limonene (20.0%), – Affouet et al. (2012)
terpinolene (42.6%)
Gabon Oleoresin α-Pinene (10.7%), sabinene (19.2%), – Engonga et al. (2012)
(HD) limonene (52.1%)
Lolodorf, Cameroon Oleoresin p-Cymene (9.8%), limonene (42.7%), Weakly antifungal, Ambindei et al. (2014)
(HD) α-terpineol (34.4%) disk diffusion assay
(Aspergillus flavus,
MIC 1800 μg mL−1;
Aspergillus niger, MIC
2800 μg mL−1;
Aspergillus fumigatus,
113
MIC 1300 μg mL−1)
(continued)
Table 4.5 (continued)
114
Table 4.6 Chemical compositions and biological activities of Dacryodes essential oils
Dacryodes Collection Essential Major components Bioactivity of
species site oil (> 5%) essential oil Reference
D. Sebang Oleoresin α-Pinene (13.2%), Antibacterial, broth Obame
buettneri Herbarium, (HD) β-pinene (42.0%), microdilution assay et al.
(Engl.) Libreville, p-cymene (19.0%), (Shigella (2007a)
H.J. Lam Gabon terpinen-4-ol dysenteriae, MIC
(27.3%) 2500 μg mL−1)
Gabon Fruit (HD) α-Pinene (29.2%), – Cravo
β-pinene (7.7%), et al.
limonene (24.3%), (1992)
α-copaene (5.2%),
germacrene D
(5.4%)
D. edulis University of Fruit (HD) α-Pinene (8.8%), – Onocha
(G. Don) Ibadan, myrcene (45.3%), et al.
H.J. Lam Nigeria α-terpineol + (1999)
germacrene D
(12.5%)
University of Leaf (HD) β-Caryophyllene – Onocha
Ibadan, (26.4%), germacrene et al.
Nigeria D (7.5%), palmitic (1999)
acid (12.7%)
University of Bark (HD) α-Thujene + – Onocha
Ibadan, α-pinene (25.2%), et al.
Nigeria limonene (12.5%), (1999)
γ-terpinene (8.6%),
terpinen-4-ol
(25.6%)
University of Root (HD) α-Pinene (7.3%), – Onocha
Ibadan, β-pinene (8.7%), et al.
Nigeria α-phellandrene (1999)
(26.5%), limonene
(10.2%),
β-phellandrene
(6.6%), p-cymene
(6.6%)
Ngaoundere, Fruit α-Pinene (60.3%), – Jirovetz
Cameroon (headspace β-pinene (8.2%), et al.
SPME) myrcene (15.0%) (2003)
Ngaoundere, Fruit (HD) α-Pinene (22.3%), – Jirovetz
Cameroon β-pinene (13.7%), et al.
α-phellandrene (2003)
(10.8%), limonene
(7.2%), (2E,4E)-
decadienal (6.7%)
(continued)
116 A. DeCarlo et al.
Table 4.6 (continued)
Dacryodes Collection Essential Major components Bioactivity of
species site oil (> 5%) essential oil Reference
Ngaoundere, Seed (HD) α-Pinene (21.5%), – Jirovetz
Cameroon β-pinene (19.7%), et al.
α-phellandrene (2003)
(12.1%), limonene
(27.5%)
Sebang Oleoresin α-Pinene (17.5%), Weakly Obame
Herbarium, (HD) sabinene (21.8%), antibacterial (MIC et al.
Libreville, p-cymene (11.3%), ≥1%) (2008)
Gabon limonene (5.7%),
γ-terpinene (5.8%),
terpinen-4-ol
(19.8%)
Etoug-Ebe, Leaf (HD) trans-Carveol Weakly Riwom
Yaoundé, (11.8%), elemol antibacterial et al.
Cameroon (29.2%), spathulenol (Bacillus cereus, (2015)
(6.3%), Salmonella typhi,
caryophyllene oxide Staphylococcus
(5.1%), ishwarone aureus, Shigella
(15.3%) sp., Escherichia
coli, MIC
18.8 mg mL−1)
D. edulis Etoug-Ebe, Bark (HD) α-Thujene (14.9%), Weakly Riwom
(G. Don) Yaoundé, β-phellandrene antibacterial et al.
H.J. Lam Cameroon (8.7%), p-cymene (Bacillus cereus, (2015)
(35.1%), trans- Salmonella typhi,
carveol (22.6%), Staphylococcus
β-elemene (5.2%) aureus, Shigella
sp., Escherichia
coli, MIC
50 mg mL−1)
Etoug-Ebe, Oleoresin α-Thujene (28.6%), Weakly Riwom
Yaoundé, (HD) α-phellandrene antibacterial et al.
Cameroon (27.1%), (Bacillus cereus, (2015)
β-phellandrene Salmonella typhi,
(10.2%), p-cymene Staphylococcus
(30.3%) aureus, Shigella
sp., Escherichia
coli, MIC
200 mg mL−1)
D. Gabon Fruit (HD) Limonene (6.9%), – Cravo
igaganga α-copaene (15.5%), et al.
Aubrév. & β-elemene (6.5%), (1992)
Pellegr. β-caryophyllene
(8.3%), α-humulene
(13.8%), germacrene
D (8.1%)
4 The Essential Oils of the Burseraceae 117
The genus Santiria consists of about 24 species of tall resiniferous trees distributed
in the Old World tropics (Mabberley 2008). Santiria trimera (Oliv.) Aubrév. (syn.
Pachylobus trimerus (Oliv.) Guillaumin), “Krio,” is a very large dioecious tree
found in the tropical rainforests of West Africa (from Sierra Leone to Nigeria, and
extending to Zaïre) (Bikanga et al. 2010). S. trimeria is one of the important medici-
nal species in this area for its antiseptic properties. The tree bark has a balsamic
odor and yields an oleoresin. In Gabon, the bark is used traditionally for wound
healing and for treating infectious diseases, while in São Tomé and Príncipe islands,
it is used for the treatment of pulmonary problems including tuberculosis and vene-
real diseases (Martins et al. 2003; Bikanga et al. 2010). The powdered bark is used
to treat yaws and to treat children’s whooping cough when mixed with palm oil and
salt. The bark is also employed as a purgative and vermifuge. A decoction of the
bark is used in vapor baths to treat fever and eczema. S. trimera bark extract con-
tains more than 60% terpenes with antimicrobial properties and is considered a
good source of lanostane derivatives including 20(R),24(E)-6β-acetoxy-3-oxo-9β-
lanosta-7,24-dien-26-oic acid and 6β-acetoxy-3,23-dioxo-9β-20β-lanost-7,24-dien-
26-oic acid (da Silva et al. 1990). The bark essential oils are rich in monoterpenoids.
A sample from Fraternidade, São Tomé and Príncipe, was dominated by α-pinene
(66.6%) and β-pinene (20.0%) (Martins et al. 2003), while a bark essential oil from
Franceville, Gabon, showed α-pinene (51.5%), β-pinene (5.8%), terpinen-4-ol
(8.5%), and α-terpineol (16.2%) (Bikanga et al. 2010). The leaf essential oil from
Gabon was dominated by the sesquiterpene hydrocarbons β-caryophyllene (14.9%)
and α-humulene (34.6%), along with α-pinene (9.4%) and humulene epoxide II
(5.6%) (Bikanga et al. 2010). Both leaf essential oil and bark oil possess weak anti-
microbial effects with the bark oil being more active (Martins et al. 2003; Bikanga
et al. 2010). In addition to its medicinal uses, the wood of S. trimeria is used in
Gabon for carving and for the production of personal items, musical instruments,
and toys.
There are around 18 species of Trattinnickia found in Central America and northern
South America (Daly 1999; Mabberley 2008; Daly and Melo 2017). Like other
members of the family, Trattinnickia produces oleoresins rich in tirucallane, ursane,
4 The Essential Oils of the Burseraceae 119
and oleanane triterpenoids (Lima et al. 2004). The Tembé people of the Amazon use
the resins (breu) from Protium and Trattinnickia to make ceremonial smoke or as
medicine for treatment of skin infections and parasites and to relieve nasal conges-
tion (Plowden et al. 2002). The resin of T. aspera (Standl.) Swart is apparently used
by white-nosed coatis (Nasua narica) in Panama to rub into their own fur and/or
that of conspecifics (Gompper and Hoylman 1993).
Very little research has been published on the essential oil compositions or
biological activities of Trattinnickia. The hydrodistilled essential oil from the
oleoresin of T. rhoifolia Willd., collected from the Adolfo Ducke Biological
Reserve, Amazonas, Brazil, has been analyzed. The major components in the resin
oil were the monoterpenoids α-pinene (23–25%), α-phellandrene (4.3–8.1%),
α-terpinene (4.3–5.8%), p-cymene (40–49%), β-phellandrene (7.6–8.7%), trans-
dihydro-α-terpineol (4.2–6.4%), and α-terpineol (1.7–5.4%) (Ramos et al. 2003).
The essential oils from T. rhoifolia branches, on the other hand, were dominated by
sesquiterpenoids, α-cubebene (12.4%), α-copaene (16.4%), β-caryophyllene
(29.6%), cis-calamenene (5.3%), δ-cadinene (15.1%), and 1-epi-cubenol (5.1%)
(de Carvalho et al. 2009).
The genus Protium is the largest genus of Burseraceae in the Neotropics, with about
150 species (Mabberley 2008). Some Protium species are found in Madagascar and
Malaysia (Mabberley 2008). The important characteristic feature of Protium spe-
cies, like their Old World counterparts Boswellia and Commiphora, is the abun-
dance of aromatic resinous exudates from wounds in the bark. The resins of Protium
species are known as “copal” in Spanish (Stacey et al. 2006) and “breu” in
Portuguese (Siani et al. 2017). Protium oleoresins have been characterized based on
their age and color as well as volatile and nonvolatile chemical constituents (Siani
et al. 2012; da Silva et al. 2013; Siani et al. 2017). Throughout their ranges, Protium
species have been used by native peoples to treat various diseases and conditions,
including wounds, skin infections, toothache, headache, pain, rheumatism, and
coughs and colds (Morton 1981; Schultes and Raffauf 1990; Rüdiger et al. 2007;
Lago et al. 2016). For example, native people of the Unini River communities in the
Amazon forest biome burn the oleoresin of P. amazonicum (Cuatrec.) Daly and
inhale the smoke to relieve headache and anxiety, while the oleoresin of P. decan-
drum (Aubl.) Marchand is used to treat skin problems such as boils and wounds
(Santos et al. 2012; Lago et al. 2016). The Yucatan Mayas used the resin of P. copal
Engl. as a styptic on infections, wounds, and sores (Morton 1981; Duke et al. 2009).
The resins of Protium species are also used as varnishes and calking and burned as
incense (Morton 1981; Duke et al. 2009).
120 A. DeCarlo et al.
Table 4.7 Chemical compositions and biological activities of Protium essential oils
Protium Collection Essential Major components Bioactivity of
species site oil (>5%) essential oil Reference
P. altsonii Erepecuru Aged p-Cymene (16.3%), – da Silva
Sandwith River, oleoresin γ-gurjunene (5.2%), et al.
Oriximiná, (HD), γ-cadinene (9.5%) (2016)
Brazil “black
breu”
Adolfo Oleoresin α-Pinene (11.0%), – Zoghbi
Ducke Forest (HD) p-cymene (31.5%), et al.
Reserve, p-menthene (13.1%), (2005)
Amazonas, trans-dihydro-α-
Brazil terpineol (25.8%)
P. Quito, Fresh (−)-δ-3-Carene Antifungal Satyal
amazonicum Ecuador oleoresin (47.9%), limonene (Cryptococcus et al.
(Cuatrec.) (HD) (5.1%), α-terpineol neoformans, MIC (2017)
Daly (5.5%) 156 μg mL−1;
Candida
albicans, MIC
313 μg mL−1)
(continued)
4 The Essential Oils of the Burseraceae 121
Table 4.7 (continued)
Protium Collection Essential Major components Bioactivity of
species site oil (>5%) essential oil Reference
P. apiculatum Porto Alegre Oleoresin p-Menthane (12.2%), – Silva
Swart Farm, (hexane p-cymene (36.3%) et al.
Amazonas extract) (2009)
state, Brazil
P. bahianum Guadalupe, Fruit α-Pinene (34.0%), Acaricidal Pontes
Daly Pernambuco, (HD) β-pinene (10.3%), (Tetranychus et al.
Brazil γ-terpinene (7.3%), urticae, LC50 (2010)
dillapiole (10.6%) 9.07 mL L−1 of
air after 24 h)
Guadalupe Leaf α-Pinene (7.8%), Acaricidal Pontes
Biological (HD) β-cubebene (16.5%), (Tetranychus et al.
Reserve, aromadendrene urticae, LC50 (2010)
Pernambuco, (20.3%), cis-β- 3.54 mL L−1 of
Brazil guaiene (9.9%), air after 24 h)
α-cadinene (10.1%)
Guadalupe Fresh Tricyclene (11.4%), Acaricidal Pontes
Biological oleoresin β-pinene (6.6%), (Tetranychus et al.
Reserve, (HD) α-phellandrene urticae, LC50 (2007b)
Pernambuco, (14.0%), p-cymene 9.08 μL L−1 of air
Brazil (18.3%), after 48 h)
β-phellandrene
(9.1%), terpinen-4-ol
(7.4%)
Guadalupe Aged (E)-β-Santalol acetate Acaricidal Pontes
Biological oleoresin (83.1%) (Tetranychus et al.
Reserve, (HD) urticae, LC50 (2007b)
Pernambuco, 7.45 μL L−1 of air
Brazil after 72 h)
P. Cocorná, Fruit α-Pinene (2.3–9.5%), – Carvajal
colombianum Colombia (HD) sabinene (51.8– et al.
Cuatrec. 70.6%), α-terpinene (2016)
(2.3–5.0%),
γ-terpinene (4.2–
7.4%), terpinen-4-ol
(6.0–13.4%)
San Luis, Fruit α-Thujene (9.4– Antifungal Carvajal
Colombia (HD) 20.4%), α-pinene (Fusarium et al.
(17.5–25.5%), oxysporum, MIC (2016)
sabinene (7.7–15.0%), 625 μg mL−1)
β-pinene (4.0–5.0%),
limonene (21.5–
32.7%), p-mentha-
2,4(8)-diene
(trace-7.6%)
(continued)
122 A. DeCarlo et al.
Table 4.7 (continued)
Protium Collection Essential Major components Bioactivity of
species site oil (>5%) essential oil Reference
P. Adolpho Leaf α-Copaene (19.6%), – de
crassipetalum Ducke Forest (HD) β-caryophyllene Carvalho
Cuatrec. Reserve, (16.4%), spathulenol et al.
Amazonas, (13.9%), τ-cadinol (2013)
Brazil (5.5%)
Adolpho Stem α-Copaene (15.2%), – de
Ducke Forest (HD) β-caryophyllene Carvalho
Reserve, (10.1%), trans-α- et al.
Amazonas, bergamotene (6.2%), (2013)
Brazil (E)-β-farnesene
(9.2%), ar-curcumene
(10.2%), β-bisabolene
(5.3%), δ-cadinene
(6.3%), khusimone
(7.9%)
P. decandrum Museu Aerial α-Pinene (78.6%), – Zoghbi
(Aubl.) Paraense parts β-pinene (5.1%), et al.
Marchand Emílio (HD) limonene (7.3%) (2005)
Goeldi,
Belém, Pará,
Brazil
Adolfo Leaf Terpinen-4-ol – de
Ducke Forest (HD) (33.0%), Carvalho
Reserve, β-caryophyllene et al.
Amazonas, (22.8%) (2010)
Brazil
Adolfo Stem Terpinen-4-ol – de
Ducke Forest (HD) (13.2%), trans-α- Carvalho
Reserve, bergamotene (22.0%), et al.
Amazonas, caryophyllene oxide (2010)
Brazil (10.5%)
Adolfo Aged cis-α-Bergamotene – de
Ducke Forest oleoresin (6.5%), Carvalho
Reserve, (HD) β-caryophyllene et al.
Amazonas, (5.9%), trans-α- (2010)
Brazil bergamotene (47.7%),
(E)-β-farnesene
(5.5%), ar-curcumene
(5.2%)
Erepecuru Aged δ-3-Carene + – da Silva
River, oleoresin iso-sylvestrene et al.
Oriximiná, (HD), (40.9%), p-cymene (2016)
Brazil “black (13.4%), limonene +
breu” β-phellandrene
(20.3%)
(continued)
4 The Essential Oils of the Burseraceae 123
Table 4.7 (continued)
Protium Collection Essential Major components Bioactivity of
species site oil (>5%) essential oil Reference
Erepecuru Aged α-Pinene (19.0%), – da Silva
River, oleoresin α-phellandrene et al.
Oriximiná, (HD), (21.0%), p-cymene (2016)
Brazil “white (32.4%), limonene +
breu” β-phellandrene
(12.0%)
P. elegans Adolfo Leaf β-Caryophyllene – de
Engl. Ducke Forest (HD) (35.9%), α-humulene Carvalho
Reserve, (12.6%), β-selinene et al.
Amazonas, (5.9%), caryophyllene (2009)
Brazil oxide (27.1%)
Adolfo Stem β-Caryophyllene – de
Ducke Forest (HD) (6.8%), caryophyllene Carvalho
Reserve, oxide (55.8%) et al.
Amazonas, (2009)
Brazil
P. Porto Alegre Oleoresin α-Pinene (20.9%), – Silva
grandifolium Farm, (hexane p-cymene (55.8%), et al.
Engl. Amazonas extract) α-cubebene (14.6%) (2009)
state, Brazil
P. hebetatum Porto Alegre Oleoresin α-Pinene (12.2%), – Silva
Daly Farm, (hexane p-cymene (14.2%), et al.
Amazonas extract) α-cubebene (14.4%) (2009)
state, Brazil
P. Crato, Ceara, Fruit α-Pinene (71.2%), – Bandeira
heptaphyllum Brazil (HD) β-pinene (8.6%), et al.
(Aubl.) limonene (5.2%) (2001)
Marchand
Timon, Fruit (Z)-β-Ocimene – Citó et al.
Maranhão (HD) (5.0%), limonene (2006)
state, Brazil (92.7%)
Tamandaré Fruit α-Terpinene (47.6%), Acaricidal Pontes
Beach, (HD) α-terpinyl acetate (Tetranychus et al.
Pernambuco, (5.0%) urticae, LC50 (2007a)
Brazil 6.85 μL L−1 of air
after 72 h)
Crato, Ceara, Leaf Myrcene (18.6%), – Bandeira
Brazil (HD) β-caryophyllene et al.
(18.6%), α-humulene (2001)
(8.0%),
bicyclogermacrene
(7.3%)
(continued)
124 A. DeCarlo et al.
Table 4.7 (continued)
Protium Collection Essential Major components Bioactivity of
species site oil (>5%) essential oil Reference
Timon, Leaf (E)-β-Ocimene – Citó et al.
Maranhão (HD) (15.7%), α-copaene (2006)
state, Brazil (5.2%),
β-caryophyllene
(32.1%), viridiflorene
(14.6%), germacrene
B (16.7%)
Manaus, Leaf Terpinolene (15.5%), – Zoghbi
Amazonas, (SD) β-elemene (22.1%), et al.
Brazil β-caryophyllene (1995)
(11.1%), α-humulene
(7.2%)
Tamandaré Leaf α-Copaene (7.3%), Acaricidal Pontes
Beach, (HD) 9-epi-(E)- (Tetranychus et al.
Pernambuco, caryophyllene urticae, LC50 (2007a)
Brazil (21.4%), trans- 10.0 μL L−1 of air
isolongifolanone after 72 h)
(10.3%), 14-hydroxy-
9-epi-(E)-
caryophyllene
(16.7%)
Manaus, Stem Terpinolene (40.3%), – Zoghbi
Amazonas, (SD) β-elemene (9.0%) et al.
Brazil (1995)
Valença, Aerial α-Pinene (40.3%), Gastroprotection Araujo
Bahia state, parts α-phellandrene (Wistar rat, ED50 et al.
Brazil (SD) (10.3%), δ-3-carene 23.6 mg kg−1) (2011)
(5.8%), p-cymene
(9.6%), m-mentha-
1,8-diene (8.9%),
p-mentha-1,4(8)-
diene (12.1%)
Timon, Oleoresin α-Phellandrene Anti- Amaral
Maranhão (HD) (10.0%), (E)-β- inflammatory et al.
state, Brazil ocimene (11.8%), (Wistar rat paw (2009)
p-cymene (10.8%), edema, ED50
limonene (50.0%), 75.1 mg kg−1)
1,8-cineole (10.9%)
Guriri, São Oleoresin Tricyclene (11.1%), Antibacterial Pinto
Mateus, (HD) p-cymene (26.7%), (Streptococcus et al.
Espírito terpinolene (35.8%), mutans, MIC (2015)
Santo, Brazil p-cymen-8-ol (10.1%) 0.13 μg mL−1)
Teresina, PI, Oleoresin δ-3-Carene (5.1%), Vasorelaxant (rat Mobin
Brazil (HD) p-cymene (17.0%), upper mesenteric et al.
limonene (34.5%), artery ring, IC50 (2017)
1,8-cineole (20.6%), 316 μg mL−1)
α-terpineol (9.8%)
(continued)
4 The Essential Oils of the Burseraceae 125
Table 4.7 (continued)
Protium Collection Essential Major components Bioactivity of
species site oil (>5%) essential oil Reference
Timon, MA, Oleoresin α-Phellandrene – Mobin
Brazil (HD) (7.0%), p-cymene et al.
(26.9%), limonene (2017)
(28.9%), α-terpineol
(18.4%)
Timon, Oleoresin α-Phellandrene Antinociceptive Rao et al.
Maranhão (HD) (10.4%), α-terpinene (capsaicin mouse (2007)
state, Brazil (13.7%), 1,8-cineole paw assay,
(58.7%), γ-terpineol 50 mg kg−1; rat
(7.7%) tail flick assay,
100 mg kg−1)
Porto Alegre Oleoresin α-Pinene (5.6%), – Silva
Farm, (hexane p-cymene (26.4%), et al.
Amazonas extract) terpinolene (20.3%), (2009)
state, Brazil α-cubebene (5.6%),
apiole (16.2%)
Crato, Ceara, Fresh α-Pinene (10.5%), – Bandeira
Brazil oleoresin α-phellandrene et al.
(HD) (16.7%), p-cymene (2001)
(6.0%), limonene
(16.9%), terpinolene
(28.5%)
Reserva da Fresh α-Terpinene (18.0%), – Siani
Campina, oleoresin p-cymene (36.0%), et al.
Amazonas, (HD) γ-terpinene (12.0%) (1999)
Brazil
Restinga of Fresh α-Pinene (27.0%), Cytotoxic (SP2/0 Siani
Carapebus, oleoresin sabinene (11.0%), murine et al.
Atlantic (HD) myrcene (35.0%), plasmocytoma (2011)
Forest, Rio β-caryophyllene cell line)
de Janeiro, (7.2%)
Brazil
Crato, Ceara, Fresh α-Pinene (10.5%), Antimicrobial Bandeira
Brazil oleoresin α-phellandrene (Candida et al.
(HD) (16.7%), p-cymene albicans, MIC (2006)
(6.0%), limonene 1.25 μg mL−1;
(16.9%), terpinolene Klebsiella
(28.5%) pneumoniae, MIC
5.0 μg mL−1;
Proteus mirabilis,
MIC
10.0 μg mL−1;
Serratia
marcescens, MIC
5.0 μg mL−1;
Staphylococcus
aureus, MIC
1.25 μg mL−1)
(continued)
126 A. DeCarlo et al.
Table 4.7 (continued)
Protium Collection Essential Major components Bioactivity of
species site oil (>5%) essential oil Reference
Restinga of Freshly α-Pinene (8.7%), Cytotoxic Siani
Carapebus, tapped α-terpinene (6.6%), (Neuro-2a murine et al.
Atlantic oleoresin p-cymene (16.0%), neuroblastoma, (2011)
Forest, Rio (HD) limonene (5.5%), SP2/0 murine
de Janeiro, terpinolene (28.0%), plasmocytoma,
Brazil p-cymen-8-ol (5.6%) J774 murine
monocytic
macrophage cell
lines)
Reserva da Aged p-Cymene (11.0%), – Siani
Campina, oleoresin terpinolene (15.0%), et al.
Amazonas, (HD) p-cymenene (5.3%), (1999)
Brazil p-cymen-8-ol
(11.0%), dillapiole
(16.0%)
Erepecuru Aged δ-3-Carene + – da Silva
River, oleoresin iso-sylvestrene et al.
Oriximiná, (HD), (69.0%), p-cymene (2016)
Brazil “black (6.4%), limonene +
breu” β-phellandrene (5.7%)
Erepecuru Aged δ-3-Carene + – da Silva
River, oleoresin iso-sylvestrene et al.
Oriximiná, (HD), (79.5%) (2016)
Brazil “black
breu”
Erepecuru Aged δ-3-Carene + – da Silva
River, oleoresin iso-sylvestrene et al.
Oriximiná, (HD), (56.4%), p-cymene (2016)
Brazil “black (14.0%), limonene +
breu” β-phellandrene (6.8%)
Erepecuru Aged δ-3-Carene + – da Silva
River, oleoresin iso-sylvestrene et al.
Oriximiná, (HD), (14.7%), p-cymene (2016)
Brazil “black (33.0%)
breu”
P. Cruzeiro do Oleoresin α-Phellandrene – Marques
heptaphyllum Sul, Acre (HD) (7.4%), p-cymene et al.
subs. state, Brazil (39.9%), dihydro-4- (2010)
heptaphyllum carene (11.7%),
(Aubl.) tetradecane (13.4%)
Marchand
P. Adolpho Leaf α-Copaene (11.8%), – de
heptaphyllum Ducke Forest (HD) β-caryophyllene Carvalho
subs. ulei Reserve, (16.9%), germacrene et al.
(Swart) Daly Amazonas, D (7.7%), δ-cadinene (2013)
Brazil (5.4%), germacrene B
(12.8%)
(continued)
4 The Essential Oils of the Burseraceae 127
Table 4.7 (continued)
Protium Collection EssentialMajor components Bioactivity of
species site oil (>5%) essential oil Reference
Cruzeiro do OleoresinLimonene (11.9%), – Marques
Sul, Acre (HD) terpinolene (42.3%), et al.
state, Brazil p-cymen-8-ol (13.6%) (2010)
P. icicariba Carapebus, Leaf α-Terpinene – Siani
(DC.) Rio de (HD) (1.9–5.8%), et al.
Marchand Janeiro, terpinolene (4.4– (2004)
Brazil 12%), α-copaene
(7.5–12%), γ-elemene
(5.6–9.7%),
germacrene D
(14–23%),
bicyclogermacrene
(6.6–12%),
δ-cadinene (5.6–
8.3%), germacrene B
(9.3–16%)
Carapebus, Fruit α-Terpinene – Siani
Rio de (HD) (21–30%), p-cymene et al.
Janeiro state, (2.2–7.9%), (2004)
Brazil γ-terpinene (9.8–
12%), terpinolene
(33–35%), terpinen-
4-ol (3.9–6.1%)
Carapebus, Oleoresin α-Pinene (5.6– – Siani
Rio de (HD) 7.7%), p-cymene et al.
Janeiro, (20–40%), limonene (2004)
Brazil (5.8–8.0%),
α-terpinolene
(5.8–31%),
p-cymen-8-ol
(10–26%)
P. neglectum Maturin, Fresh p-Cymene (5.2%), Antibacterial, Suárez
Swart Monagas oleoresin durenol (15.6%), disk diffusion et al.
state, (HD) α-terpineol (6.9%), assay (Bacillus (2007)
Venezuela piperitenone (25.4%), subtilis,
thymol (17.5%), Staphylococcus
methyl eugenol aureus)
(9.2%)
P. occultum Erepecuru Aged α-Pinene (8.0%), – da Silva
Daly River, oleoresin p-cymene (10.4%), et al.
Oriximiná, (HD), limonene + (2016)
Brazil “white β-phellandrene
breu” (41.1%), α-terpineol
(30.9%)
(continued)
128 A. DeCarlo et al.
Table 4.7 (continued)
Protium Collection Essential Major components Bioactivity of
species site oil (>5%) essential oil Reference
P. opacum Erepecuru Aged p-Cymene (6.6%), – da Silva
Swart River, oleoresin α-neo-clovene (5.3%), et al.
Oriximiná, (HD), α-neo-callitropsene (2016)
Brazil “black (7.3%), γ-cadinene
breu” (14.4%),
P. Porto Alegre Oleoresin α-Pinene (10.8%), – Silva
paniculatum Farm, (hexane p-cymene (6.4%), et al.
Engl. Amazonas extract) β-caryophyllene (2009)
state, Brazil (6.8%)
P. Museu Aerial α-Pinene (31.7%), – Zoghbi
pilosissimum Paraense parts α-phellandrene et al.
Engl. Emílio (HD) (24.1%), p-cymene (2005)
Goeldi, (31.2%)
Belém, Pará,
Brazil
Adolpho Leaf α-Copaene (11.6%), – de
Ducke Forest (HD) β-caryophyllene Carvalho
Reserve, (12.6%), et al.
Amazonas, β-sesquiphellandrene (2013)
Brazil (24.3%), (E)-nerolidol
(6.9%)
Adolpho Stem Caryophyllene oxide – de
Ducke Forest (HD) (9.8%), selin-11-en- Carvalho
Reserve, 4α-ol (56.5%) et al.
Amazonas, (2013)
Brazil
P. Adolpho Stem β-Caryophyllene – de
polybotryum Ducke Forest (HD) (15.0%), Carvalho
(Turcz.) Engl. Reserve, caryophyllene oxide et al.
Amazonas, (11.9%), khusimone (2013)
Brazil (35.9%)
P. Museu Aerial Sabinene (56.3%), – Zoghbi
spruceanum Paraense parts γ-terpinene (6.3%), et al.
(Benth.) Engl. Emílio (HD) terpinen-4-ol (12.2%), (2005)
Goeldi, β-caryophyllene
Belém, Pará, (10.9%)
Brazil
P. strumosum Adolfo Oleoresin α-Pinene (6.3%), – Zoghbi
Daly Ducke Forest (HD) limonene (75.5%), et al.
Reserve, α-terpineol (7.7%) (2005)
Amazonas,
Brazil
Porto Alegre Oleoresin p-Cymene (26.1%), – Silva
Farm, (hexane limonene (14.7%) et al.
Amazonas extract) (2009)
state, Brazil
(continued)
4 The Essential Oils of the Burseraceae 129
Table 4.7 (continued)
Protium Collection Essential Major components Bioactivity of
species site oil (>5%) essential oil Reference
Erepecuru Aged α-Pinene (57.7%), – da Silva
River, oleoresin β-pinene (9.3%), et al.
Oriximiná, (HD), p-cymene (9.2%), (2016)
Brazil “white limonene +
breu” β-phellandrene
(10.8%)
P. Manaus, Oleoresin α-Pinene (8.5%), – Zoghbi
subserratum Amazonas, (hexane α-phellandrene et al.
(Engl.) Engl. Brazil extract) (20.8%), (1998)
β-phellandrene
(56.3%)
P. Manaus, Leaf Limonene (24.2%), – Zoghbi
unifoliolatum Amazonas, (SD) α-copaene (6.2%), et al.
Engl. Brazil β-caryophyllene (1993)
(37.5%), α-humulene
(9.9%)
source origins may or may not be correct. Nevertheless, they were included for a
completeness of this review. It is hoped that this review will become a reference
source for future studies on oils from this fascinating plant family.
Acknowledgments This work was carried out as part of the activities of the Aromatic Plant
Research Center (APRC, https://aromaticplant.org/). The authors are grateful to dōTERRA
International (https://www.doterra.com/US/en) for financial support of the APRC. A careful editing
of the chapter by Brian M. Lawrence is acknowledged by the authors.
Conflicts of Interest The authors declare no conflicts of interest. The funding sponsor, dōTERRA
International, played no role in preparation or in the decision to publish this review.
References
Abdel Wahab SM, Aboutabl EA, El-Zalabani SM, Fouad HA, De Pooter HL, El-Fallaha B (1987)
The essential oil of olibanum. Planta Med 53(4):382–384
Acıduman A, Tuzcu K, Belen D (2017) One of the earliest accounts of head wounds in Firdaws
al-Ḥikma by al-Ṭabar from the 9th century. World Neurosurg 107:834–838
Adam ME, Selim SA (2013) Antimicrobial activity of essential oil and methanol extract from
Commiphora molmol (Engl.) resin. Int J Curr Microbiol Appl Sci 2(12):1–6
Adams RP (2007) Identification of essential oil components by gas chromatography/mass spec-
trometry, 4th edn. Allured Publishing, Carol Stream, IL
Adorisio S, Fierabracci A, Gigliarelli G, Muscari I, Cannarile L, Liberati AM, Marcotullio MC,
Riccardi C, Curini M, Robles Zepeda RE, Delfino DV (2017) The hexane fraction of Bursera
microphylla A. Gray induces p21-mediated antiproliferative and proapoptotic effects in human
cancer-derived cell lines. Integr Cancer Ther 16(3):426–435
Affouet KM, Tonzibo ZF, Attioua BK, Chalchat JC (2012) Chemical investigations of volatile oils
from aromatic plants growing in Côte d’Ivoire: Harisonia abyssinica Oliv., Canarium schwer-
furthii Engl., Zanthoxylum gilletti (De wild) Waterm. and Zanthoxylum zanthoxyloides Lam.
Anal Chem Lett 2(6):367–372
Agbor GA, Kuate D, Oben JE (2007) Medicinal plants can be good sources of antioxidants: case
study in Cameroon. Pak J Biol Sci 10(4):537–544
Ageel AM, Tariq M, Mossa JS, Ai-Yahya MA, AI-Said MS (1987) Plants used in Saudi folk medi-
cine. King Saud University Press, Riyadh
Ahmed HH, Abd-Rabou AA, Hassan AZ, Kotob SE (2015) Phytochemical analysis and anti-
cancer investigation of Boswellia serrata bioactive constituents in vitro. Asian Pac J Cancer
Prev 16(16):7179–7188
Ajibesin KK, Ekpo BA, Bala DN, Essien EE, Adesanya SA (2008) Ethnobotanical survey of Akwa
Ibom State of Nigeria. J Ethnopharmacol 115:387–408
Alaamri MMH (2012) Distribution Boswellia sacra in Dhofar Mountains, Sultinate of Oman:
economic value and environmental role. J Life Sci 6(6):632–636
Al-Harrasi A, Al-Saidi S (2008) Phytochemical analysis of the essential oil from botanically certi-
fied oleogum resin of Boswellia sacra (Omani luban). Molecules 13:2181–2189
Al-Harrasi A, Ali L, Hussain J, Ur Rehman N, Mehjabeen AM, Al-Rawahi A (2014) Analgesic
effects of crude extracts and fractions of Omani frankincense obtained from traditional
medicinal plant Boswellia sacra on animal models. Asian Pac J Trop Med 7(Suppl 1):
S485–S490
Ali BZ (2007) Evaluation of myrrh (Commiphora molmol) essential oil against some storage
fungi. J Al-Nahrain Univ 10(2):107–111
4 The Essential Oils of the Burseraceae 131
Ayédoun MA, Sohounhloué DK, Menut C, Lamaty G, Molangul T, Cassanova J, Tomi F (1998)
Aromatic plants of Tropical West Africa. VI. α-Oxobisabolene as main constituent of the
leaf essential oil of Commiphora africana (A. Rich.) Engl. from Benin. J Essent Oil Res
10(1):105–107
Bakır B, Him A, Özbek H, Düz E, Tütüncü M (2008) Investigation of the anti-inflammatory and
analgesic activities of β-caryophyllene. Int J Essent Oil Therap 2:41–44
Bandaranayake WM (1980) Terpenoids of Canarium zeylanicum. Phytochemistry 19:255–257
Bandeira PN, Machado MIL, Cavalcanti FS, Lemos TLG (2001) Essential oil composi-
tion of leaves, fruits and resin of Protium heptaphyllum (Aubl.) March. J Essen Oil Res
13(1):33–34
Bandeira PN, Fonseca AM, Costa SMO, Lins MUDS, Pessoa ODL, Monte FJQ, Nogueira NAP,
Lemos TLG (2006) Antimicrobial and antioxidant activities of the essential oil of resin of
Protium heptaphyllum. Nat Prod Commun 1(2):117–120
Baratta MT, Dorman HJD, Deans SG, Figueiredo AC, Barrosi JG, Ruberto G (1998) Antimicrobial
and antioxidant properties of some commercial essential oils. Flavour Fragr J 13:235–244
Basar S (2005) Phytochemical investigations on Boswellia species. Dissertation, Universität
Hamburg
Basar S, Koch A, König WA (2001) A verticillane-type diterpene from Boswellia carterii essential
oil. Flavour Fragr J 16(5):315–318
Başer KHC, Demirci B, Dekebo A, Dagne E (2003) Essential oils of some Boswellia spp., myrrh
and opopanax. Flavour Fragr J 18:153–156
Becerra JX, Noge K (2010) The Mexican roots of the Indian lavender tree. Acta Bot Mex
(91):27–36
Becerra JX, Noge K, Venable DL (2009) Macroevolutionary chemical escalation in an ancient
plant–herbivore arms race. Proc Natl Acad Sci U S A 106(43):18062–18066
Bekana D, Kebede T, Assefa M, Kassa H (2014) Comparative phytochemical analyses of resins of
Boswellia species (B. papyrifera (Del.) Hochst., B. neglecta S. Moore, and B. rivae Engl.) from
northwestern, southern, and southeastern Ethiopia. ISRN Anal Chem 2014:374678
Ben Yehoshua S, Rachmilevitch S, Amiel E, Ofir R, Dudai N, Soloway E (2015) Revival of the
extinct balm of Gilead in Istrael: studying its anti-cancer activity. Acta Hortic (1088):509–514
Benelli G, Rajeswary M, Vijayan P, Senthilmurugan S, Alharbi NS, Kadaikunnan S, Khaled JM,
Govindarajan M (2017) Boswellia ovalifoliolata (Burseraceae) essential oil as an eco-friendly
larvicide? Toxicity against six mosquito vectors of public health importance, non-target mos-
quito fishes, backswimmers, and water bugs. Environ Sci Pollut Res 2017:1–8
Berenbaum MR, Zangerl AR (2008) Facing the future of plant-insect interaction research: Le
retour à la “raison d’être”. Plant Physiol 146(3):804–811
Beyra A, León MC, Iglesias E, Ferrándiz D, Herrera R, Volpato G, Godínez D, Guimarais M,
Álvarez R (2004) Estudios etnobotánicos sobre plantas medicinales en la provincia de
Camagüey (Cuba). An Jardín Bot Madrid 61(2):185–204
Bikanga R, Makani T, Agnaniet H, Obame LC, Abdoul-Latif FM, Lebibi J, Menut C (2010)
Chemical composition and biological activities of Santiria trimera (Burseraceae) essential oils
from Gabon. Nat Prod Commun 5(6):961–964
Birkett MA, Al Abassi S, Kröber T, Chamberlain K, Hooper AM, Guerin PM, Pettersson J, Pickett
JA, Slade R, Wadhams LJ (2008) Antiectoparasitic activity of the gum resin, gum haggar, from
the East African plant, Commiphora holtziana. Phytochemistry 69(8):1710–1715
Bouquet A (1969) Féticheurs et Médecines Traditionnelles du Congo (Brazzaville). Office de la
Recherche Scientifique et Technique Outre-Mer, Paris
Brown D (2001) New encyclopedia of herbs & their uses, revised ed. Dorling Kindersley, London
Budowski G, Russo RO (1993) Live fence posts in Costa Rica. J Sustain Agric 3(2):65–87
Burkill HM (2000) Useful plants of West Tropical Africa, vol. 4: Families M - R., 2nd edn. Royal
Botanic Gardens, Kew
Butzer KW (1981) Rise and fall of Axum, Ethiopia: a geo-archaeological interpretation. Am Antiq
46(3):471–495
4 The Essential Oils of the Burseraceae 133
Daly DC, Harley MM, Martínez-Habibe MC, Weeks A (2010) Burseraceae. In: Kubitzki K (ed)
The families and general of vascular plants, vol. X. Flowering plants. Eudicots. Springer,
Heidelberg, pp 76–104
Dannaway FR (2010) Strange fires, weird smokes and psychoactive combustibles: entheogens and
incense in ancient traditions. J Psychoactive Drugs 42(4):485–497
de Carvalho LE, Pinto DS, Lima MP, Marques MOM, Facanali R (2009) The chemistry of essen-
tial oils of Crepidospermum rhoifolium, Trattinnickia rhoifolia and Protium elegans of the
Amazon region. J Essent Oil Bear Plants 12(1):92–96
de Carvalho LE, Pinto DS, Magalhães LAM, Lima MP, Marques MOM, Facanali R (2010)
Chemical constituents of essential oil of Protium decandrum (Burseraceae) from western
Amazon. J Essent Oil Bear Plants 13:181–184
de Carvalho LE, Magalhães LAM, Lima MP, Marques MOM, Facanali R (2013) Essential oils of
Protium of the Adolpho Ducke Forest Preserve: Protium crassipetalum, P. heptaphyllum subs.
ulei, P. pilosissimum and P. polybotryum. J Essent Oil Bear Plants 16(4):551–554
de Rapper S, Van Vuuren SF, Kamatou GPP, Viljoen AM, Dagne E (2012) The additive and syn-
ergistic antimicrobial effects of select frankincense and myrrh oils – a combination from the
pharaonic pharmacopoeia. Lett Appl Microbiol 54(4):352–358
Dekebo A, Zewdu M, Dagne E (1999) Volatile oils of frankincense from Boswellia papyrifera.
Bull Chem Soc Ethiopia 13(1):93–96
De-Nova JA, Medina R, Montero JC, Weeks A, Rosell JA, Olson ME, Eguiarte LE, Magallón
S (2011) Insights into the historical construction of species-rich Mesoamerican seasonally
dry tropical forests: the diversification of Bursera (Burseraceae, Sapindales). New Phytol
193:276–287
Dictionary of Natural Products (2017) Dictionary of natural products on DVD. CRC Press, Boca
Raton, FL
Dike IP, Obembe OO, Adebiyi FE (2012) Ethnobotanical survey for potential anti-malarial plants
in south-western Nigeria. J Ethnopharmacol 144(3):618–626
Djilani A, Dicko A (2012) The therapeutic benefits of essential oils. In: Bouayed J (ed) Nutrition,
well-being and health. InTech, Rijeka, Croatia, pp 155–178
Dongmo PMJ, Tchoumbougnang F, Ndongson B, Agwanande W, Sandjon B, Zollo PHA, Menut
C (2010) Chemical characterization, antiradical, antioxidant and anti-inflammatory potential of
the essential oils of Canarium schweinfurthii and Aucoumea klaineana (Burseraceae) growing
in Cameroon. Agric Biol J North Am 1(4):606–611
Dounchaly AR, Jaimand K, Mozaffari S (2016) Comparison of essential oils compositions of
Boswellia carteri Birdwood as a food and non-food in different distillation from Iranian mar-
ket. J Med Plants By Prod 1:39–44
Dozmorov MG, Yang Q, Wu W, Wren J, Suhail MM, Woolley CL, Young DG, Fung K, Lin H
(2014) Differential effects of selective frankincense (ru xiang) essential oil versus non-selective
sandalwood (tan xiang) essential oil on cultured bladder cancer cells: a microarray and bioin-
formatics study. Chin Med 9:18
Dudai N, Shachter A, Satyal P, Setzer WN (2017) Chemical composition and monoterpenoid
enantiomeric distribution of the essential oils from apharsemon (Commiphora gileadensis).
Medicines 4(3):66
Duke JA (1970) Ethnobotanical observations on the Chocó Indians. Econ Bot 24(3):344–366
Duke JA, Bogenschutz-Godwin MJ, Ottesen AR (2009) Duke’s handbook of medicinal plants of
Latin America. CRC Press, Boca Raton, FL
Ehrlich PR, Raven PH (1964) Butterflies and plants: a study in coevolution. Evolution
18(4):586–608
El Ashry ESH, Rashed N, Salama OM, Saleh A (2003) Components, therapeutic value and uses of
myrrh. Pharmazie 58(3):163–168
Eldridge J (1975) Bush medicine in the Exumas and Long Island, Bahamas: a field study. Econ
Bot 29(4):307–332
Engonga PE, Abdoul-Latif FM, Obame LC, Mewono L (2012) Volatile constituents of Canarium
schweinfurthii Engl. essential oil from Gabon. Int J Agri Sci 2(3):200–203
4 The Essential Oils of the Burseraceae 135
Epstein I (1990) The Babylonian Talmud, 30 volumes. Bloch Publishing Company, New York
Erukainure OL, Mopuri R, Oyebode OA, Koorbanally NA, Islam MS (2017) Dacryodes edulis
enhances antioxidant activities, suppresses DNA fragmentation in oxidative pancreatic and
hepatic injuries; and inhibits carbohydrate digestive enzymes linked to type 2 diabetes. Biomed
Pharmacother 96:37–47
Eshete A (2011) The frankincense tree of ethiopia: ecology, productivity and population dynamics.
Dissertation, Wageningen University
Eshete A, Teketay D, Hulten H (2005) The socio-economic importance and status of populations
of Boswellia papyrifera (Del.) Hochst. in northern Ethiopia: the case of North Gonder Zone.
Forests Trees Livelihoods 15:55–74
Eshete A, Sterck FJ, Bongers F (2012) Frankincense production is determined by tree size and
tapping frequency and intensity. Forest Ecol Manag 274:136–142
Eslamieh J (2016) The genus Commiphora; preservation through horticulture. A Book’s Mind,
Fort Collins, CO
Eslamieh J (2017) Cultivation of Boswellia, 2nd edn. A Book’s Mind, Fort Collins, CO
Espinosa D, Llorente J, Morrone JJ (2006) Historical biogeographical patterns of the species of
Bursera (Burseraceae) and their taxonomic implications. J Biogeogr 33:1945–1958
Evans WC (2009) Trease and evans pharmacognosy, 16th edn. Saunders Ltd, Edinburgh
Evans PH, Becerra JX (2006) Non-terpenoid essential oils from Bursera chemapodicta. Flavour
Fragr J 21:616–618
Farah MH (2008) Non-Timber Forest Production (NTFP) extraction in arid environments: land-use
change, frankincense production and the sustainability of Boswellia sacra in Dhofar (Oman).
Dissertation, University of Arizona
Ferrara L (2016) Phytotherapy as a preventive and adjuvant for the rhinitis. IOSR J Pharm
6(1):6–11
Fichtl R, Addi A (1994) Honeybee flora of ethiopia: some 500 common herbs, shrubs and trees.
Margraf Publishers GmbH, Weikersheim
Firn RD, Jones CG (2003) Natural products – a simple model to explain chemical diversity. Nat
Prod Rep 20(4):382–391
Fonkeng LS, Mouokeu RS, Tume C, Njateng GSS, Kamcthueng MO, Ndonkou NJ, Kuiate
JR (2015) Anti-Staphylococcus aureus activity of methanol extracts of 12 plants used in
Cameroonian folk medicine. BMC Res Notes 8:710
Fraternale D, Sosa S, Ricci D, Genovese S, Messina F, Tomasini S, Montanari F, Marcotullio MC
(2011) Anti-inflammatory, antioxidant and antifungal furanosesquiterpenoids isolated from
Commiphora erythraea (Ehrenb.) Engl. resin. Fitoterapia 82(4):654–661
Frawley D, Lad V (2001) The yoga of herbs: an ayurvedic guide to herbal medicine, 2nd edn.
Lotus Press, Twin Lakes, WI
Gazzaneo LRS, de Lucena RFP, de Albuquerque UP (2005) Knowledge and use of medicinal plants
by local specialists in an region of Atlantic Forest in the state of Pernambuco (Northeastern
Brazil). J Ethnobiol Ethnomed 8:9
Gebrehiwot K, Muys B, Haile M, Mitloehner R (2003) Introducing Boswellia papyrifera (Del.)
Hochst and its non-timber forest product, frankincense. Int Forest Rev 5(4):348–353
Gebrehiwot M, Asres K, Bisrat D, Mazumber A, Lindemann P, Bucar F (2015) Evaluation of the
wound healing property of Commiphora guidottii Chiov. ex. Guid. BMC Complemen Altern
Med 15:282
Gebremedhin T (1997) Boswellia papyrifera (Del.) Hochst. from Western Tigray: opportunities,
constrains and seed germination responses. Dissertation, Swedish University of Agricultural
Sciences
Gemedo-Dalle T, Maass BL, Isselstein J (2005) Plant biodiversity and ethnobotany of Borana
pastoralists in southern Oromia, Ethiopia. Econ Bot 59(1):43–65
Gertsch J, Leonti M, Raduner S, Racz I, Chen JZ, Xie XQ, Altmann KH, Karsak M, Zimmer A (2008)
Beta-caryophyllene is a dietary cannabinoid. Proc Natl Acad Sci U S A 105(26):9099–9104
Getahon A (1976) Some common medicinal and poisonous plants used in ethiopian folkmedicine.
Addis Abeba University, Addis Abebe
136 A. DeCarlo et al.
Ghelardini C, Galeotti N, Mannelli LDC, Mazzanti G, Bartolini A (2001) Local anaesthetic activ-
ity of β-caryophyllene. Farmaco 56:387–389
Giang PM, Konig WA, Son PT (2006) Chemical composition of the resin essential oil of Canarium
album from Vietnam. Chem Nat Comp 42(5):523–524
Gigliarelli G, Becerra JX, Curini M, Marcotullio MC (2015) Chemical composition and biological
activities of fragrant Mexican copal (Bursera spp.). Molecules 20:22383–22394
Gompper ME, Hoylman AM (1993) Grooming with Trattinnickia resin: possible pharmaceutical
plant use by coatis in Panama. J Trop Ecol 9(4):533–540
Groenendijk P, Eshete A, Sterck FJ, Zuidema PA, Bongers F (2012) Limitations to sustainable
frankincense production: blocked regeneration, high adult mortality and declining populations.
J Appl Ecol 49:164–173
Groom N (1981) Frankincense and myrrh: a study of the Arabian incense trade. Longman Group,
London
Gupta M, Rout PK, Misra LN, Gupta P, Singh N, Darokar MP, Saikia D, Singh SC, Bhakuni RS
(2017) Chemical composition and bioactivity of Boswellia serrata Roxb. essential oil in rela-
tion to geographical variation. Plant Biosyst 151(4):623–629
Habeeb SM, El-Namaky AH, Salama MA (2009) Efficiency of Allium cepa and Commiphora
molmol as a larvicidal agent against fourth stage larvae of Culex pipiens (Diptera: Culicidae).
Am Euras J Agric Environ Sci 5(2):196–203
Hakkim FL, Al-Buloshi M, Al-Sabahi J (2015) Frankincense derived heavy terpene cocktail boost-
ing breast cancer cell (MDA-MB-231) death in vitro. Asian Pac J Trop Biomed 5(10):824–828
Hameed HA (1983) Avicenna’s tract on cardiac drugs and essays on Arab cardiotherapy. Hamdard
Foundation, Karachi
Hamm S, Bleton J, Connan J, Tchapla A (2005) A chemical investigation by headspace SPME and
GC–MS of volatile and semi-volatile terpenes in various olibanum samples. Phytochemistry
66:1499–1514
Hammer KA, Carson CF, Riley TV (1999) Antimicrobial activity of essential oils and other plant
extracts. J Appl Microbiol 86:985–990
Hanuš LO, Řezanka T, Dembitsky VM, Moussaieff A (2005) Myrrh – Commiphora chemistry.
Biomed Pap 149(1):3–28
Hanuš LO, Rosenthal D, Řezanka T, Dembitsky VM, Moussaief A (2008) Fast and easy GC/MS
identification of myrrh resins. Pharmaceut Chem J 42(12):719–720
Hausen BM, Reichling J, Harkenthal M (1999) Degradation products of monoterpenes are the
sensitizing agents in tea tree oil. Am J Cont Dermat 10(2):68–77
Higgs L (1978) Bush medicine in the Bahamas. Nassau Guardian, Nassau
Hoe VB, Siong KH (1999) The nutritional value of indigenous fruits and vegetables in Sarawak.
Asia Pac J Clin Nutr 8(1):24–31
Hull BZ (2008) Frankincense, myrrh, and spices: the oldest global supply chain? J Macromark
28(3):275–288
İşcan G, Kırımer N, Demirci F, Demirci B, Noma Y, Başer KHC (2012) Biotransformation of
(−)-(R)-α-phellandrene: antimicrobial activity of its major metabolite. Chem Biodivers
9:1525–1532
Iwu MM (2014) Handbook of African medicinal plants, 2nd edn. CRC Press, Boca Raton, FL
Jirovetz L, Buchbauer G, Geissler M, Ngassoum MB, Parmentier M (2003) Pulp aroma com-
pounds of untreated, boiled and roasted African pear [Dacryodes edulis (G. Don) H.J. Lam]
fruits from Cameroon by HS-SPME analysis coupled with GC/FID and GC/MS. Eur Food Res
Technol 218:40–43
Junor GO, Porter RBR, Facey PC, Yee TH (2007) Investigation of essential oil extracts from four
native Jamaican species of Bursera for antibacterial activity. West Indian Med J 56:22–25
Junor GAO, Porter RBR, Yee TH (2008a) The leaves, bark and fruits of Bursera simaruba (L.)
Sarg. from Jamaica. J Essent Oil Res 20(5):426–429
Junor GAO, Porter RBR, Yee TH, Williams LAD (2008b) Chemical composition and insecticidal
activity of the essential oils from Bursera hollickii (Britton) Found in Jamaica. J Essent Oil
Res 20(6):560–566
4 The Essential Oils of the Burseraceae 137
Junor GAO, Porter RBR, Yee TH (2010a) Chemical composition of essential oils from the aerial
parts of Jamaican Bursera lunanii Spreng. J Essent Oil Res 22(6):602–606
Junor GAO, Porter RBR, Yee TH, Waugh T (2010b) The volatile constituents from the
leaves, bark and fruits of Bursera aromatica (Proctor) found in Jamaica. J Essent Oil Res
22(1):19–23
Kalenda DT, Missang CE, Kinkela TT, Krebs HC, Renard CMGC (2002) New developments in
the chemical characterisation of the fruit of Dacryodes edulis (G. Don) H.J. Lam. Forests Trees
Livelihoods 12(1–2):119–123
Karnick CR (1995) Pharmacopoeial standards of herbal plants. Sri Satguru Publications, Delhi
Kasali AA, Adio AM, Oyedeji AO, Eshilokun AO, Adefenwa M (2002) Volatile constituents of
Boswellia serrata Roxb. (Burseraceae) bark. Flavour Fragr J 17:462–464
Katsuyama S, Mizoguchi H, Kuwahata H, Komatsu T, Nagaoka K, Nakamura H, Bagetta G,
Sakurada T, Sakurada S (2013) Involvement of peripheral cannabinoid and opioid receptors in
β-caryophyllene-induced antinociception. Eur J Pain 17(5):664–675
Khalid SA (1983) Chemistry of the Burseraceae. In: Waterman PG, Grundon MF (eds) Chemistry
and chemical taxonomy of the Rutales. Academic Press, New York, pp 281–299
Kitchen KA (1971) Punt and how to get there. Orientalia 40(2):184–207
Kohoude MJ, Gbaguidi F, Agbani P, Ayedoun MA, Cazaux S, Bouajila J (2017) Chemical compo-
sition and biological activities of extracts and essential oil of Boswellia dalzielii leaves. Pharm
Biol 55(1):33–42
Kong KW, Chew LY, Prasad KN, Lau CY, Ismail A, Sun J, Hosseinpoursarmadi B (2011) Nutritional
constituents and antioxidant properties of indigenous kembayau (Dacryodes rostrata (Blume)
H. J. Lam) fruits. Food Res Int 44(7):2332–2338
Koudou J, Abena AA, Ngaissona P, Bessière JM (2005) Chemical composition and pharmacologi-
cal activity of essential oil of Canarium schweinfurthii. Fitoterapia 76:700–703
Koudou J, Obame LC, Kumulungui BS, Edou P, Figueredo G, Chalchat JC, Traore AS (2009)
Volatile constituents and antioxidant activity of Aucoumea klaineana Pierre essential oil.
African J Pharm Pharmacol 3(6):323–326
Kubmarawa D, Ogunwande IA, Okorie DA, Olawore NO, Kasali AA (2006) Constituents of the
essential oils of Boswellia dalzielii Hutch. from Nigeria. J Essent Oil Res 18(2):119–120
Lago JHG, Tezoto J, Yazbek PB, Cassas F, Santos JFL, Rodrigues E (2016) Exudates used as
medicine by the “caboclos river-dwellers” of the Unini River, AM, Brazil – classification based
in their chemical composition. Rev Bras Farmacogn 26:379–384
Langenheim JH (2003) Plant resins: chemistry, evolution, ecology, and ethnobotany. Timber Press,
Portland, OR
Lebaka PAR, Ratnam KV, Lepakshi BM, Bhumi NR, Lebaka VR (2015) Chemical profile, antioxi-
dant and antimicrobial activity of essential oils from Boswellia ovalifoliolata Bal. et. Henry. Int
J Pharmaceut Clin Res 7(1):96–101
Lemenih M, Kassa H (2011) Management guide for sustainable production of frankincense.
Center for International Forestry Research, Bogor
Lemenih M, Teketay D (2003) Frankincense and myrrh resources of Ethiopia: II. Medicinal and
industrial uses. Ethiop J Sci 26(2):161–172
Li ZF, Li K, Li FY, Chen H, Zhao HH, Du YY, Li QP (2015) [GC-MS analysis and antioxidant
activity of volatile oil from leaves of Canarium pimela Leenh. in Rongxian county]. J South
Agric 46(2):317–321
Liang GY, Bates CD, Gray AI, Waterman PG (1988a) The volatile oil of the oleo resin of Aucoumea
klaineana collected in Gabon. Planta Med 54(4):368–369
Liang GY, Gray AI, Waterman PG (1988b) Tirucallane and oleanane triterpenes from the resin of
Aucoumea klaineana. Phytochemistry 27(7):2283–2286
Lima MP, Braga PAC, Macedo ML, da Silva MFGF, Ferreira AG, Fernandes JB, Vieira PC (2004)
Phytochemistry of Trattinnickia burserifolia, T. rhoifolia, and Dacryodes hopkinsii: chemosys-
tematic implications. J Braz Chem Soc 15(3):385–394
Lippi MM, Giuliani C, Gonnelli T, Bini LM (2011) Floral color changes in Boswellia sacra Flueck.
(Burseraceae): a dialogue between plant and pollinator. Flora 206(9):821–826
138 A. DeCarlo et al.
Lis-Balchin M (2010) Aromatherapy with essential oils. In: Başer KHC, Buchbauer G (eds)
Handbook of essential oils. CRC Press, Boca Raton, FL, pp 549–583
Lis-Balchin M, Hart S (1997) A preliminary study of the effect of essential oils on skeletal and
smooth muscle in vitro. J Ethnopharmacol 58:183–187
Lugo AE, Wadsworth FH (1990) Dacryodes excelsa Vahl Tabonuco. In: Burns RM, Honkala BH
(eds) Silvics of North America, vol. 2, Hardwoods. United States Department of Agriculture,
Washington, DC, pp 284–287
Mabberley DJ (2008) Mabberley’s plant-book, 3rd edn. Cambridge University Press, Cambridge
Maděra P, Paschová Z, Ansorgová A, Vrškový B, Lvončík S, Habrová H (2017) Volatile com-
pounds in oleo-gum resin of Socotran species of Burseraceae. Acta Univ Agric Silvic Mendel
Brun 65(1):73–90
Mahboubi M, Kashani LMT (2016) The anti-dermatophyte activity of Commiphora molmol.
Pharm Biol 54(4):720–725
Mahr D (2012) Commiphora: an introduction to the genus. Cactus Succulent J 84(3):140–154
Manguro LOA, Mukonyi KM, Githiomi JK (1996) Bisabolenes and furanosesquiterpenoids of
Kenyan Commiphora kua resin. Planta Med 62:84–85
Manzano Santana P, Miranda M, Gutiérrez Y, Garcia G, Orellana T, Orellana A (2009) Efecto
antiinflamatorio y composición química del aceite de ramas de Bursera graveolens Triana &
Planch. (palo santo) de Ecuador. Rev Cub Plant Med 14(3):45–53
Marcotullio MC, Santi C, Mwankie GNOM, Curini M (2009) Chemical composition of the essen-
tial oil of Commiphora erythraea. Nat Prod Commun 4(12):1751–1754
Marongiu B, Piras A, Porcedda S, Scorciapino A (2005) Chemical composition of the essential
oil and supercritical CO2 extract of Commiphora myrrha (Nees) Engl. and of Acorus calamus.
J Agric Food Chem 53:7939–7943
Marongiu B, Piras A, Porcedda S, Tuveri E (2006) Extraction of Santalum album and Boswellia
carterii Birdw. volatile oil by supercritical carbon dioxide: influence of some process param-
eters. Flavour Fragr J 21:718–724
Marques DD, Sartori RA, Lemos TLG, Machado LL, de Souza JSN, Monte FJQ (2010) Chemical
composition of the essential oils from two subspecies of Protium heptaphyllum. Acta Amazon
40(1):227–230
Martins AP, Salgueiro LR, Gonçalves MJ, Proença da Cunha A, Vila R, Cañigueral S (2003)
Essential oil composition and antimicrobial activity of Santiria trimera bark. Planta Med
69(1):77–79
Mathe C, Culioli G, Archier P, Vieillescazes C (2004) Characterization of archaeological frankin-
cense by gas chromatography–mass spectrometry. J Chromatogr A 1023:277–285
Medzegue MJ, Stokes A, Gardrat C, Grelier S (2013) Analysis of volatile compounds in Aucoumea
klaineana oleoresin by static headspace/gas chromatography/mass spectrometry. J Nat Prod
(India) 6:81–89
Mengistu T (2011) Physiological ecology of the frankincense tree. Dissertation, Wageningen
University
Mengistu T, Sterck FJ, Anten NPR, Bongers F (2012) Frankincense tapping reduced photosyn-
thetic carbon gain in Boswellia papyrifera (Burseraceae) trees. For Ecol Manag 278:1–8
Mertens M, Buettner A, Kirchhoff E (2009) The volatile constituents of frankincense – a review.
Flavour Fragr J 24:279–300
Michie CA, Cooper E (1991) Frankincense and myrrh as remedies in children. J Roy Soc Med
84(10):602–605
Mies BA, Lavranos JJ, James GJ (2000) Frankincense on Soqotra Island (Boswellia, Burseraceae;
Yemen). Cactus Succulent J 72(5):265–278
Mikhaeil BR, Maatooq GT, Badria FA, Amer MMA (2003) Chemistry and immunomodulatory
activity of frankincense oil. Z Naturforsch C 58(3–4):230–238
Miller A (2015) Boswellia sp. A., The IUCN Red List of Threatened Species 2015:
e.T44811A69307248
Miller AG, Morris M (2004) Ethnoflora of the Soqotra Archipelago. Royal Botanic Gardens,
Edinburgh
4 The Essential Oils of the Burseraceae 139
Pinto FE, Heringer OA, Silva MA, Uggere T, Ribeiro JS, Lenz D, Campos F, Lessa R, Endringer
DC (2015) Stability and disinfecting proprieties of the toothbrush rinse of the essential oil of
Protium heptaphyllum. African J Pharm Pharmacol 9(6):173–181
Plowden C, Uhl C, Oliveira FA (2002) Breu resin harvest by Tembé Indians and its dependence on
a bark-boring beetle. In: Stepp JR, Wyndham FS, Zarger RK (eds) Ethnobiology and biocultural
diversity. International Society of Ethnobiology, Athens, GA, pp 365–380
Pontes WJT, de Oliveira JCG, da Cámara CAG, Lopes ACHR, Gondim Junior MGC, de Oliveira
JV, Barros R, Schwartz MOE (2007a) Chemical composition and acaricidal activity of the
leaf and fruit essential oils of Protium heptaphyllum (Aubl.) Marchand (Burseraceae). Acta
Amazon 37(1):103–110
Pontes WJT, de Oliveira JCS, da Camara CAG, Lopes ACHR, Gondim Júnior MGC, de Oliveira
JV, Schwartz MOE (2007b) Composition and acaricidal activity of the resin’s essential oil of
Protium bahianum Daly against two spotted spider mite (Tetranychus urticae). J Essent Oil
Res 19:379–383
Pontes WJT, Silva JMO, da Camara CAG, Gondim-Júnior MGC, Oliveira JV, Schwartz MOE
(2010) Chemical composition and acaricidal activity of the essential oils from fruits and leaves
of Protium bahianum Daly. J Essent Oil Res 22(3):279–283
Prakash B, Mishra PK, Kedia A, Dubey NK (2014) Antifungal, antiaflatoxin and antioxidant
potential of chemically characterized Boswellia carterii Birdw essential oil and its in vivo
practical applicability in preservation of Piper nigrum L. fruits. LWT Food Sci Technol
56(2):240–247
Price C, Forshaw R, Chamberlain A, Nicholson P (2016) Mummies, magic and medicine in
Ancient Egypt: multidisciplinary essays for Rosalie David. Manchester University Press,
Manchester
Queiroga CL, Duarte MCT, Ribeiro BB, Melillo P, de Magalhães PM (2007) Linalool production
from the leaves of Bursera aloexylon and its antimicrobial activity. Fitoterapia 78:327–328
Ragunathan M, Senthamarai R (2013) Anti-inflammatory activity of essential oil of Canarium
strictum Roxb. Iranian J Pharmaceut Sci 9(2):13–21
Rajagopal MS (2014) Canarium patentinervium Miq. (Burseraceae Kunth.): a phytochemical and
pharmacological study. Dissertation, University of Nottingham (Malaysia Campus)
Raju AJS, Lakshmi PV, Ramana KV, Chandra PH (2012) Entomophily, ornithophily and ane-
mochory in the self-incompatible Boswellia ovalifoliolata Bal. & Henry (Burseraceae), an
endemic and endangered medicinally important tree species. J Threat Taxa 4(7):2673–2684
Ramos MFS, Guimarães AC, Siani AC (2003) Volatile monoterpenes from the oleoresin of
Trattinnickia rhoifolia. Biochem Systemat Ecol 31:309–311
Rao VS, Maia JL, Oliveira FA, Lemos TLG, Chaves MH, Santos FA (2007) Composition and anti-
nociceptive activity of the essential oil from Protium heptaphyllum resin. Nat Prod Commun
2(12):1199–1202
Reddy CS, Sudhakar KN, Murthy EN, Raju VS (2009) Traditional medicinal plants in Seshachalam
Hills, Andhra Pradesh, India. J Med Plants Res 3(5):408–412
Rey-Valeirón C, Guzmán L, Saa LR, López-Vargas J, Valarezo E (2017) Acaricidal activity of
essential oils of Bursera graveolens (Kunth) Triana & Planch and Schinus molle L. on unen-
gorged larvae of cattle tick Rhipicephalus (Boophilus) microplus (Acari: Ixodidae). J Essent
Oil Res 29(4):344–350
Richards LA, Dyer LA, Forister ML, Smilanich AM, Dodson CD, Leonard MD, Jeffrey CS (2015)
Phytochemical diversity drives plant–insect community diversity. Proc Natl Acad Sci U S A
112(35):10973–10978
Rijkers T, Ogmazghi W, Wessel M, Bongers F (2006) The effect of tapping for frankincense on
sexual reproduction in Boswellia papyrifera. J Appl Ecol 43:1188–1195
Riwom SH, Ndoye Foe FMC, Nyegue MA, Ambe Wanki R, Voundi Olugu S, Etoa FX (2015)
Chemical composition and in vitro antibacterial activity of the essential oils of the leaves, resin
and stem-barks of Dacryodes edulis (G. Don) H. J Lam growing in Cameroon on diarrhea
associated strains. J Appl Pharmaceut Sci 5(10):6–11
142 A. DeCarlo et al.
Rosales-Ovares KM, Cicció-Alberti JF (2002) Aceite volátil de los frutos de Bursera simaruba
(L.) Sarg. (Burseraceae) de Costa Rica. Ing Ciencia Quím 20(2):60–61
Roy NK, Deka A, Bordoloi D, Mishra S, Kumar AP, Sethi G, Kunnumakkara AB (2016) The poten-
tial role of boswellic acids in cancer prevention and treatment. Cancer Lett 377:74–86
Rüdiger AL, Siani AC, Viega Junior VF (2007) The chemistry and pharmacology of the South
America genus Protium Burm. f. (Burseracea). Pharmacogn Rev 1(1):93–104
Sadhasivam S, Palanivel S, Ghosh S (2016) Synergistic antimicrobial activity of Boswellia serrata
Roxb. ex Colebr. (Burseraceae) essential oil with various azoles against pathogens associated
with skin, scalp and nail infections. Lett Appl Microbiol 63:495–501
Saeed MA, Sabir AW (2004) Antibacterial activities of some constituents from oleo-gum-resin of
Commiphora mukul. Fitoterapia 75:204–208
Santos JFL, Pagani E, Ramos J, Rodrigues E (2012) Observations on the therapeutic practices of
riverine communities of the Unini River, AM, Brazil. J Ethnopharmacol 142:503–515
Sarup P, Bala S, Kamboj S (2015) Pharmacology and phytochemistry of oleo-gum resin of
Commiphora wightii (guggulu). Scientifica 2015:138039
Satyal P, Pappas RS (2016) First reporting on the chemistry and biological activity of a novel
Boswellia chemotype: the methoxy alkane frankincense. Global J Sci Front Res B Chem
16(2):1–9
Satyal P, Powers CN, Parducci R, Mcfeeters RL, Setzer WN (2017) Chemical composition,
enantiomeric distribution, and antifungal activity of the oleoresin essential oil of Protium
amazonicum from Ecuador. Medicines 4(4):70
Sawamura M, Son US, Choi HS, Kim MSL, Phi NTL, Fears M, Kumagai C (2004) Compositional
changes in commercial lemon essential oil for aromatherapy. Int J Aromather 14(1):27–63
Schillaci D, Arizza V, Dayton T, Camarda L, Di Stefano V (2008) In vitro anti-biofilm activity of
Boswellia spp. oleogum resin essential oils. Lett Appl Microbiol 47(5):433–438
Schultes RE, Raffauf RF (1990) The healing forest: medicinal and toxic plants of Northwest
Amazonia. Dioscorides Press, Portland, OR
Semenya SS, Maroyi A (2012) Medicinal plants used by the Bapedi traditional healers to treat diar-
rhoea in the Limpopo Province, South Africa. J Ethnopharmacol 144(2):395–401
Setzer WN (2014) Leaf and bark essential oil compositions of Bursera simaruba from Monteverde,
Costa Rica. Am J EssentOils Nat Prod 1(3):34–36
Sharma ML, Chowdhury AR, Raina RM (1996) Essential oil from the leaves of Bursera serrata
Wall. J Non Timber For Prod 3:86–88
Shen T, Lou HX (2008) Bioactive constituents of myrrh and frankincense, two simultaneously
prescribed gum resins in Chinese traditional medicine. Chem Biodivers 5:540–553
Shen T, Li GH, Wang XN, Lou H (2012) The genus Commiphora: a review of its traditional uses,
phytochemistry and pharmacology. J Ethnopharmacol 142(2):319–330
Siani AC, Ramos MFS, Guimarães AC, Susunaga GS, Zoghbi MGB (1999) Volatile constituents
from oleoresin of Protium heptaphyllum (Aubl.) March. J Essent Oil Res 11:72–74
Siani AC, Garrido IS, Monteiro SS, Carvalho ES, Ramos MFS (2004) Protium icicariba as a
source of volatile essences. Biochem Systemat Ecol 32:477–489
Siani AC, Ramos MFS, Monteiro SS, Ribeiro-dos-Santos R, Soares ROA (2011) Essential oils
of the oleoresins from Protium heptaphyllum growing in the Brazilian southeastern and their
cytotoxicity to neoplastic cell lines. J Essent Oil Bear Plants 14(3):373–378
Siani AC, Nakamura MJ, Tappin MRR, Monteiro SS, Guimarães AC, Ramos MFS (2012) Chemical
composition of South American Burseraceae non-volatile oleoresins and preliminary solubility
assessment of their commercial blend. Phytochem Anal 23:529–539
Siani AC, Moraes R, Veiga Junior VF (2017) Toward establishing the productive chain for trit-
erpene-based Amazonian oleoresins as valuable non-timber forest products. Open J Forestry
7:188–208
Silva JRDA, Zoghbi MGB, Pinto AC, Godoy RLO, Amaral ACF (2009) Analysis of the hexane
extracts from seven oleoresins of Protium species. J Essent Oil Res 21(4):305–308
Singh BB, Mishra LC, Vinjamury SP, Aquilina N (2003) The effectiveness of Commiphora mukul
for osteoarthritis of the knee: an outcomes study. Altern Ther Health Med 9(3):74–79
4 The Essential Oils of the Burseraceae 143
Singh B, Kumar R, Bhandari S, Pathania S, Lal B (2007) Volatile constituents of natural Boswellia
serrata oleo-gum-resin and commercial samples. Flavour Fragr J 22:145–147
Stacey RJ, Cartwright CR, McEwan C (2006) Chemical characterization of ancient Mesoamerican
“copal” resins: preliminary results. Archaeometry 48(2):323–340
Su S, Wang T, Duan JA, Zhou W, Hua YQ, Tang YP, Yu L, Qian DW (2011) Anti-inflammatory
and analgesic activity of different extracts of Commiphora myrrha. J Ethnopharmacol
134:251–258
Suárez AI, Compagnone RS, Acosta D, Vásquez L, Diaz B, Canelón DJ (2007) Chemical compo-
sition and antimicrobial activity of the essential oil from oleoresin of Protium neglectum S. J
Essent Oil Bear Plants 10(1):70–75
Suhail MM, Wu W, Cao A, Mondalek FG, Fung KM, Shih PT, Fang YT, Woolley C, Young G, Lin
HK (2011) Boswellia sacra essential oil induces tumor cell-specific apoptosis and suppresses
tumor aggressiveness in cultured human breast cancer cells. BMC Complement Altern Med
11:129
Sultana A, Ur Rahman K, Padmaja AR, Ur Rahman S (2013) Boswellia serrata Roxb. A tra-
ditional herb with versatile pharmacological activity: a review. Int J Pharmaceut Sci Res
4(6):2106–2117
Sunnichan VG, Mohan Ram HY, Shivanna KR (2005) Reproductive biology of Boswellia serrata,
the source of salai guggul, an important gum-resin. Bot J Linnean Soc 147:73–82
Sylvestre M, Longtin APA, Legault J (2007) Volatile leaf constituents and anticancer activity of
Bursera simaruba (L.) Sarg. essential oil. Nat Prod Commun 2(12):1273–1276
Tadesse M, Demissew S (1992) Medicinal Ethiopian plants: inventory, identification, and classi-
fication. In: Botany 2000: East and Central Africa. Plants used in Africal traditional medicine
as practiced in Ethiopia and Uganda. NAPRECA, Addis Ababa University, Addis Ababa,
pp 1–19
Tcheghebe OT, Seukep AJ, Tatong FN (2016) A review on traditional uses, phytochemi-
cal composition and pharmacological profile of Canarium schweinfurthii Eng. Nature Sci
14(11):17–22
Tee LH, Yang B, Nagendra KP, Ramanan RN, Sun J, Chan ES, Tey BT, Azlan A, Ismail A, Lau
CY, Jiang Y (2014) Nutritional compositions and bioactivities of Dacryodes species: a review.
Food Chem 165:247–255
Tee LH, Yang B, Tey BT, Chan ES, Azlan A, Ismail A, Sun J, Lau CY, Palanisamy UD, Ramanan
RN, Prasad KN (2017) Valorization of Dacryodes rostrata fruit through the characterization of
its oil. Food Chem 235:257–264
Tessier AM, Delaveau P, Piffault N (1982) Oléo-résine d’Aucoumea klaineana. Planta Med
44(4):215–217
Thang TD, Luu HV, Dung NX (2004) Chemical composition of the leaf oil of Canarium benga-
lense Roxb. from Vietnam. J Essent Oil Bear Plants 7(1):43–48
Thang TD, Dai DN, Luong NX, Ogunwande IA (2014) Constituents of essential oils from the
leaves, stem barks and resins of Canarium parvum Leen., and Canarium tramdenanum Dai et
Yakovl. (Burseracea) grown in Vietnam. Nat Prod Res 28(7):461–466
Thomson LAJ, Evans B (2006) Canarium indicum var. indicum and C. harveyi (canarium nut).
In: Elevitch CR (ed) Species profiles for pacific island agroforestry. Permanent Agriculture
Resources, Hōlualoa, HI
Thulin M (1999) Flora of Somalia: volume 2, Angiospermae (Tiliaceae-Apiaceae). Royal Botanic
Gardens, Kew
Thulin M, Claeson P (1991) The botanical origin of scented myrrh (blssabol or habak hadi). Econ
Bot 45(4):487–494
Thulin M, Warfa AM (1987) The frankincense trees (Boswellia spp., Burseraceae) of northern
Somalia and southern Arabia. Kew Bull 42(3):487–500
Thulin M, Beier BA, Razafimandimbison SG, Banks HI (2008) Ambilobea, a new genus from
Madagascar, the position of Aucoumea, and comments on the tribal classification of the frank-
incense and myrrh family (Burseraceae). Nordic J Bot 26:218–229
144 A. DeCarlo et al.
Tolera M, Menger D, Sass-Klaassen U, Sterck FJ, Copini P, Bongers F (2013) Resin secre-
tory structures of Boswellia papyrifera and implications for frankincense yield. Ann Bot
111:61–68
Trevizan LNF, do Nascimento KF, Santos JA, Kassuya CAL, Cardoso CAL, Vieira MC, Moreira
FMF, Croda J, Formagio ASN (2016) Anti-inflammatory, antioxidant and anti-Mycobacterium
tuberculosis activity of viridiflorol: the major constituent of Allophylus edulis (A. St.-Hil.,
A. Juss. & Cambess.) Radlk. J Ethnopharmacol 192:510–515
Tucker AO (1986) Frankincense and myrrh. Econ Bot 40:425–433
Tucker AO, Maciarello MJ, Brown RC, Landrum LR, Lafferty D (2009) Essential oils from the
oleo-gum-resins of elephant tree or torote (Bursera microphylla A. Gray, Burseraceae) from
Arizona. J Essent Oil Res 21(1):57–58
Turek C, Stintzing FC (2012) Impact of different storage conditions on the quality of selected
essential oils. Food Res Int 46(1):341–353
Tyldesley J (1998) Hatchepsut: the female pharaoh, revised. Penguin Books, London
Ur Rehman N, Hussain H, Csuk R, Abbas G, Green IR, Al-Harrasi A (2017) A norterpenoid
and tripenoids from Commiphora mukul: isolation and biological activity. Z Naturforsch B
72(1):11–15
Van Vuuren SF, Kamatou GPP, Viljoen AM (2010) Volatile composition and antimicrobial activity
of twenty commercial frankincense essential oil samples. S African J Bot 76:686–691
Villanueva MA, Torres RC, Başer KHC, Özek T, Kürkçüoğlu M (1993) The composition of Manila
elemi oil. Flavour Fragr J 8:35–37
Walsh ME, Reis D, Jones T (2010) Integrating complementary and alternative medicine: use of
myrrh in wound management. J Vasc Nurs 28(3):102
Weeks A, Daly DC, Simpson BB (2005) The phylogenetic history and biogeography of the
frankincense and myrrh family (Burseraceae) based on nuclear and chloroplast sequence
data. Mol Phylogen Evol 35:85–101
Woolley CL, Suhail MM, Smith BL, Boren KE, Taylor LC, Schreuder MF, Chai JK, Casabianca
H, Haq S, Lin HK, Al-Shahri AA, Al-Hatmi S, Young DG (2012) Chemical differentiation of
Boswellia sacra and Boswellia carterii essential oils by gas chromatography and chiral gas
chromatography-mass spectrometry. J Chromatogr A 1261:158–163
Xie J, Wang S, Sun B, Zheng F (2008) Preparative separation and purification of β-caryophyllene
from leaf oil of Vitex negundo L. var. heterophylla (Franch.) Rehd. by high speed countercurrent
chromatography. J Liq Chromatogr Relat Technol 31:2621–2631
Yang SA, Jeon SK, Lee EJ, Shim CH, Lee IS (2010) Comparative study of the chemical com-
position and antioxidant activity of six essential oils and their components. Nat Prod Res
24(2):140–151
Yeo SK, Ali AY, Hayward OA, Turnham D, Jackson T, Bowen ID, Clarkson R (2016) β-Bisabolene,
a sesquiterpene from the essential oil extract of opoponax (Commiphora guidottii) exhibits
cytotoxicity in breast cancer cell lines. Phytother Res 30(3):418–425
Young DG, Chao S, Casabianca H, Bertrand MC, Minga D (2007) Essential oil of Bursera graveo-
lens (Kunth) Triana et Planch from Ecuador. J Essent Oil Res 19:525–526
Yukawa C, Iwabuchi H, Kamikawa T, Komemushi S, Sawabe A (2004) Terpenoids of the volatile
oil of Bursera graveolens. Flavour Fragr J 19:565–570
Yukawa C, Imayoshi Y, Iwabuchi H, Komemushi S, Sawabe A (2006) Chemical composition of
three extracts of Bursera graveolens. Flavour Fragr J 21:234–238
Zofou D, Tematio EL, Ntie-Kang F, Tene M, Ngemenya MN, Tane P, Titanji VPK (2013) New
antimalarial hits from Dacryodes edulis (Burseraceae) - Part I: isolation, in vitro activity, in
silico “drug-likeness” and pharmacokinetic profiles. PLoS One 8(11):e79544
Zoghbi MGB, da Cunha EVL, Filho WW (1993) Essential oil of Protium unifoliolatum
(Burseraceae). Acta Amazon 23(1):15–16
Zoghbi MGB, Maia JGS, Luz AIR (1995) Volatile constituents from leaves and stems of Protium
heptaphyllum (Aubl.) March. J Essent Oil Res 7(5):541–543
Zoghbi MGB, Andrade EHA, Santos AS, Luz AIR, Maia JGS (1998) Volatile constituents of the
resins from Protium subserratum (Engl.) Kuntz. J Essent Oil Res 10(3):325–326
4 The Essential Oils of the Burseraceae 145
Zoghbi MGB, Andrade EHA, Lima MP, Silva TMD, Daly DC (2005) The essential oils of five
species of Protium growing in the north of Brazil. J Essent Oil Bear Plants 8(3):312–317
Zúñiga B, Guevara-Fefer P, Herrera J, Contreras JL, Velasco L, Pérez FJ, Esquivel B (2005)
Chemical composition and anti-inflammatory activity of the volatile fractions from the bark of
eight Mexican Bursera species. Planta Med 71:825–828
Part II
Uses of Essential Oils in Various Industries
Chapter 5
Essential Oil of Betel Leaf (Piper betle L.):
A Novel Addition to the World Food Sector
Proshanta Guha and Sujosh Nandi
5.1 Introduction
Essential oils are also called volatile oils or ethereal oils (Guenther 1948), but the
term is a misnomer. This is because it apprises some substances which are neither
essential nor oil in relation to food sector, wherein an oil is supposed to be a mixture
of mixed fatty acids. That apart, unlike essential fatty acids, the essential oils are not
required for maintaining good health or sound mind. They are not indispensable for
the producer organisms as well because the organisms can survive and complete
their life cycle without any such oils. Though not strictly necessary, rather optional,
the essential oils can help the producer organisms in various ways such as protection
from competitors, pathogens, insects, etc. It can also help the plants in various other
ways such as reduction in transpiration losses, communication with other plants and
microbes, and attraction of the pollinating agents like insects, birds, etc., when pres-
ent in the plants. In the true sense, the word essential relates to essence that means
concentrated scent, and the word has its roots in the Latin word essentia-ae which
means perfumes (Bevilacqua et al. 2018). Therefore, any original volatile substance
which has any distinct odour or scent can be termed as essential substance.
Further, the second part of the term essential oil is the oil for which no proper
definition exists though in food sectors it represents merely a mixture of mixed fatty
acids. However, oil can ordinarily be identified by some of its principal properties
such as liquid at room temperature, lighter than water, slippery to touch, ability to
produce a greasy mark on white paper, inflammability, immiscibility in water,
saponification, hydrophobicity, solubility in organic solvents, lipophilicity, etc. For
example, edible oils (olive oil, coconut oil, mustard oil, sesame oil, etc.), but inclusion
of fuel oils (kerosene oil; petroleum, petra+oleum meaning rock oil; diesel oil; etc.),
mineral oil (liquid paraffins), lubricant oil (motor oils), etc., in the domain of oils
could make it very confusing, but still, the meaning of oil in common sense is well
understood in the realm of food production, processing, and consumption.
In the above context, the essential oils are called oils perhaps due to the fact that
they possess some of the above-mentioned empirical properties. Therefore, the sim-
plest definition of essential oil could be reduced to merely a pure scented oil or vola-
tile scented oil of direct biological origin. However, some authors have defined
essential oil as any oily and aromatic volatile liquid that can be harvested from any
part of the plant (Burt 2004; Speranza and Corbo 2010; Böhme et al. 2014). The
International Organization for Standardization (ISO), on the other hand, defined
essential oil as—“product obtained from natural raw material, either by distillation
with water and steam, or from the epicarp of citrus fruits by mechanical processing,
or by dry distillation” (quoted by Sadgrove and Jones 2015).
There are about 17,500 aromatic plants (Regnault-Roger et al. 2012) belonging
to approximately 60 families (Raut and Karuppayil 2014) producing about 3000
different essential oils, but only 300 essential oils are commercially available for
different uses such as perfumes, dentistry, agriculture, food preservation, house-
keeping, natural remedies, aromatherapy, and so on (Van de Braak and Leijten
1999; Speranza and Corbo 2010). These oils consist mainly of hydrocarbons includ-
ing some functional compounds. The number of such compounds may be as high as
300 in a single essential oil (Sandra and Bicchi 1987) though mostly restricted to
20–30 detectable compounds with the help of latest scientific technologies like
LC-MS, GC-MS, GC × GC-MS, DART-MS, NMR, and so on.
It is a common belief that essential oils are secondary metabolites of the plants
or their specific parts such as flowers (rose), buds (clove), seeds (cardamom), bark
(cinnamon), wood (sandal), peels (orange), roots (vetiver), leaf (betel leaf), etc.
However, it cannot be conclusively said that the essential oils originate only in the
plants. This is because there are many other producers of essential oils like animals
and microorganisms. The animal sources include musk deer which belong to the
family Moschidae that encompasses seven species including Moschus leucogaster,
commonly known as white-bellied musk deer or Himalayan musk deer in India
(TNEB 1974; Wikipedia 2018a). In these species, some strong odoriferous sub-
stance, muscone or 3-methylcyclopentadecanone (Fig. 5.1), is produced in the naval
Fig. 5.1 Molecular structure, formula, and weight of muscone and civetone (Rana 2015)
5 Essential Oil of Betel Leaf (Piper betle L.): A Novel Addition to the World Food… 151
gland of the adult male deer which is supposed to attract its female counterparts.
The gland, when fully grown and functional, makes the deer insane probably in
search of the source of the highly attractive fragrance, and ultimately, it dies due to
accidents by crashing against some trees, hard objects, or in any other ways. The
odoriferous substance is so strong that a grain of musk can distinctly provide fra-
grance to millions of cubic feet of air without any perceptible loss of weight (Kraft
2004). This scent is not only most penetrating but also most persistent among all the
known substances which emit odour. In India, some of the rich and wealthy persons,
kings, and emperors only could afford this musk for various purposes, such as cos-
metics, medicines, and aphrodisiac. Obviously, it was one of the most expensive
cosmetic articles in the history. Even in the present day also, it is still in use, though
rarely, for religious purposes in the Lord Shiva temples, for instance, Lord
Pashupatinath Temple in Nepal. However, use of this substance is coming down to
an end since the musk deer is facing extinction. Therefore, they are enlisted as
endangered species, and now finding a live specimen in India or Nepal is extremely
difficult. Similar is the case with another endangered animal commonly known as
civet. This carnivorous mammal looks like a big cat and is mostly found in Asia,
Europe, and Africa. There are about 15–20 species of civet which are grouped into
10–12 genera under Viverridae family (Rafferty 2012). Both the male and female
civets produce an oil-like substance in their perineal gland (pouches below the tail)
with a strong musky odour which is highly valued as fragrance and a stabilizing
agent for perfumes. When pure, the odour is strong and putrid, but once diluted it
becomes pleasant with sweet odour. The African civet (Civettictis civetta) is reared
in Africa for obtaining civet oil which contains about 2.5–3.4% civetone or
9-cycloheptadecen-1-one (Fig. 5.1) as the major odoriferous ingredient. The molec-
ular structure, formula, and weight of muscone and civetone are given in Fig. 5.1.
Apart from the animals, some microorganisms are also capable of producing
attractive odoriferous substances from which scent is manufactured. It may be a
matter of debate if such scented materials may be termed essential oil or not, but
they also originate in the lower plants (Barnett 2015). Such odoriferous substance is
extracted in Kannauj situated in the Uttar Pradesh province of India, where it is
termed as Mitti Attar meaning earthy fragrant material. Here, Mitti means earth and
Attar means concentrated odoriferous liquid in Indian language (Hindi). This earthy
smell is supposed to be due to a by-product produced by a typical bacteria or acti-
nomyces. The by-product is called geosmin (Wikipedia 2018b, c). This attar is
stored in a special leather bag called Kuppi for retaining its original fragrance.
The essential oils can be classified into various ways such as quality of aroma,
time taken for evaporation or persistence of odour, etc. The classification based on
the quality of aroma puts the fragrant and flavouring substances into different cate-
gories, such as citrus, earthy, floral, herbaceous, camphorous, medicinal, minty,
oriental, spicy, woody, etc. On the other hand, the oils, when classified on the basis
of time taken for evaporation or persistence, are categorized into three groups, such
as top note (1–2 h), middle note (2–4 h), and base note (a few days). However, in the
current scenario, the essential oils are designated directly by the name of the source
material such as rose oil, sandalwood oil, mint oil, betel leaf oil, etc.
152 P. Guha and S. Nandi
5.2 O
rigin, Taxonomy, and Nomenclature of Betel
Leaf (Piper betle L.)
History of betel leaf chewing dates back to the antiquity. However, both the archae-
ologists and anthropologists could trace it to only 7000 BC (Pradhan et al. 2013)
and the Malay Archipelago is generally recognized as the place of origin of the crop
(de Candolle 1884; Burkill 1966; Chattopadhyay and Maity 1967).
Betel vine (Fig. 5.2) is a perennial root climber which belongs to the family
Piperaceae (Guenther 1952), the black pepper family that includes several herbs,
shrubs, small trees, and hanging vines (Ferreres et al. 2014). The family Piperaceae
has 10 genera and 2000 species, of which 30 are found in India and 18 in Sri
Lanka, and 3 are endemic (Chakraborty and Shah 2011; Gupta and Singh 2016).
The taxonomic position of betel vine in the plant kingdom is given below (Pradhan
et al. 2013):
Kingdom: Plantae
Division: Magnoliophyta
Class: Magnoliopsida
Order: Piperales
Family: Piperaceae
Genus: Piper
Species: betle
Binomial Name: Piper betle L.
Synonyms: Chavica betle (L.) Miquel; Piper pinguispicum C. DC. and Koord
(MHMC 2015); Piper peepuloides Wall, Piper chavya Ham (Periyanayagam
et al. 2011)
The betel vine is also known by various other names in various countries of the
world. A list of some of the important vernacular names of the vine in different
countries of the World and also in India is given in Tables 5.1 and 5.2,
respectively.
In India, betel leaf is known by more than 150 names in various local languages,
such as Nagavalli, Nagarvel, Saptaseera, Sompatra, Tamalapaku, Tambul, Tambuli,
Vaksha Patra, Vetrilai, Voojangalata, and so on in different parts of the country
(CSIR 1969; Guha and Jain 1997). However, Paan is the most popular name of betel
leaf in all parts of India and adjacent countries such as Nepal, Pakistan, and
Bangladesh. There are about 100–150 varieties of betel leaf in the world, of which
40 are found in India and 30 in West Bengal (Maity 1989; Samanta 1994; Guha and
Jain 1997; Ravindran et al. 2002). However, on the basis of morphology and micro-
metrical traits, all these varieties can be grouped into six different categories,
namely, Bangla, Desawari, Kapoori, Khasi, Sanchi (or Chhaanchi), and Mitha
(Rawat et al. 1989).
Table 5.1 Common names of betel leaf in different languages in different countries
S. S.
no. Language (country) Name no. Language (country) Name
1 Afrikaans (Africa) Betel blad 12 Khmer (Cambodia) Sloek phne I
2 Arabic (Saudi Arabia) Tambol 13 Lao/Laotian (Laos) Mark
3 Burmese (Myanmar) Kwm rwat 14 Latin (Malta) Piperis
folium
4 Chamorro (Guam) Papulu 15 Malay (Indonesia, Daun sirih
Malaysia)
5 Dhivehi (Maldives) Foah 16 Persian (Iran) Burg-e-tanbol
6 English (Europe, USA) Betel leaf 17 Sinhalese (Sri Lanka) Bulath
7 French (France) Feuille de betel 18 Spanish (Spain) Hoja de betel
8 Filipino (Philippines) Sirang dahon 19 Tetum (East Timor) Malus, Malu
9 German (Germany) Betel blatt 20 Thai (Thailand) Phlu, Bai
pluu
10 Javanese (parts Sirih, Suruh, 21 Tok Pisin (Papua New Buai
Of Indonesia) Bodeh Guinea)
11 Kapampangan Bulung samat 22 Vietnam (Vietnamese) Trau
(Philippines)
154 P. Guha and S. Nandi
Importance and widespreadness of betel leaf can be comprehended from the fact
that it has specific synonyms in almost all languages of the world (Table 5.2). This
indicates that the betel leaf is known in almost all parts of the world where it is
either cultivated or used for different purposes after import. This practically proves
that betel leaf is obviously growing more and more popular with passage of time,
obviously due to its aromatic, medicinal, stimulant, and other beneficial attributes
contributed mostly by the essential oil present in the leaves (Guha 1997; Khanra
1997). That apart, intercontinental migration of the Asian population has also con-
tributed towards dissemination of knowledge and information about the leaves.
Betel leaf (Fig. 5.3) is a heart-shaped dorsiventral green leaf. However, the leaves
may attain various shades of green with yellow tinge or very dark green with black-
ish hue. The vine has weak cylindrical stem with green colour which becomes semi-
woody when old with earthy or mixed brown or yellow colour. The stem may have
many other shades of colour such as green with parallel red lines when young.
From each node of the stem, adventitious roots develop which enable the vine to
climb up along the host plants or the inert support (Fig. 5.2). This way, the vines
may grow 15–50 feet or more in a year with profuse branching which are removed
from time to time for improving quality of the leaves. Some varieties called Gaach
Paan or “Tree-betel vine” may grow beyond even 50 feet along the support which
5 Essential Oil of Betel Leaf (Piper betle L.): A Novel Addition to the World Food… 155
is mainly a living areca nut tree (Areca catechu L.). The leaves are petiolated, gla-
brous, and alternate. The length of the leaves may range from 5 to 20 cm and width
may range from 3 to 15 cm, which may vary beyond these ranges due to edapho-
climatic, management, and genetic factors. The leaves have one main rib (midrib)
and 4–8 additional ribs which emerge from the base (petiolar side) and converge
towards the apex. The leaf apex is acute or acuminate, and the leaf margin is entire.
The stout petiole may attain a length of 10–50% of the leaf blade. The male and
female flowers are separate on dioecious plants, and both are spiky, dense, cylindri-
cal, and off-white in colour measuring from 3 to 10 cm in length or more but with-
out any sepals or petals. Fruits are rarely produced under Indian agroclimatic
conditions.
The vine is a tropical shade-loving plant. It requires hot and humid climate.
Temperature may range from 15 to 40 °C, relative humidity from 40% to 80%, and
rainfall from 2250 to 4750 mm resembling to the ecological conditions of a tropical
forest. A well-drained fertile sandy or sandy loam or sandy clay soil with pH range
156 P. Guha and S. Nandi
of 5.6–8.2 is suitable for its cultivation. Normally, the male plants are raised for
harvesting the fresh leaves (CSIR 1969; Guha and Jain 1997). However, in low
rainfall or hot and dry areas, this crop can be cultivated with assured irrigation and
shade net technology. Otherwise also, most of the cultivated varieties except Gaach
Paan are vegetatively propagated inside a hut-like structure called Boroj or Bareja
or Bouroj, which is traditionally constructed with materials like bamboo, banana
leaves, straw, jute sticks, dry grasses, and even iron rods in recent times providing a
shady and humid environment in side. It is a voracious feeder of nutrients and water
and also requires huge investment in terms of pesticides since it is highly suscepti-
ble to insect pests and diseases. The nutritional requirement of the crop may be as
high as 600, 300, and 250 kg NPK/ha per year, and the crop requires irrigation
weekly and fortnightly during summer and winter, respectively, or sooner or later
depending upon soil and agroclimatic conditions. The initial cost of cultivation, as
was calculated during 2006, was about ₹1–2 lakh/ha at the minimum during the first
year which came down to ₹0.5–0.6 lakh/ha during the subsequent years (Guha
2006). The current values of these figures may range from four to six times higher.
However, the betel vine is cultivated in small fields, as small as 0.04 ha, which is
sufficient for providing proper employment and sufficient income for maintaining a
small family of five members in rural India (Jana 1995; SDAMM 1996).
In India, it is cultivated on 40,000–55,000 ha of land with a production worth of
₹7000–10,000 million (Rawat et al. 1989; Guha 2006; Das et al. 2016b) amounting
to about 0.20 million tons of betel leaf annually which is the highest known figure
in the world. On the other hand, two reports indicate that the crop is also cultivated
in Bangladesh with an area production of about 12,660 ha and about 0.06 million
tons (Rawat et al. 1989) and 18,247 ha and 0.10 million tons (Islam et al. 2015).
However, consolidated global area production data is not available. Therefore, com-
pilation of this requires joint efforts by all concerned governments, scientists, farm-
ers, traders, exporters, and the importers as well, but the scanty data available so far
indicate that India exports betel leaves to many Asian, African, Australian, American,
and European countries including Afghanistan, Canada, France, Germany, UAE,
UK, USA, etc. (Singh et al. 1990; Jana 1996) and earned about $4 million during
2013–2014 (TOI 2014).
The economic potentiality of the crop can be judged by the data given in the previ-
ous section (Agrotechnology of Betel Vine). Further, the potentiality of this crop
can also be judged by the fact that about 15–20 million people consume betel leaves
on a regular basis in India alone (Jana 1996) besides over two billion consumers in
other countries of the world (Jeng et al. 2002). The leaves are also used as a mark of
respect and in auspicious occasions in social, cultural, and religious events regularly
in India and many other Asian countries (Guha 2006; Sengupta and Banik 2013;
Mohanto et al. 2017). Further, it is estimated that about 20 million people derived
5 Essential Oil of Betel Leaf (Piper betle L.): A Novel Addition to the World Food… 157
Guha (2006) reported the biochemical composition of the leaves from different
sources including his own work as shown in Table 5.3.
However, previously, Gopalan et al. (1984) have reported 12 analytical data out
of 21 parameters presented in Table 5.3 which are slightly different but within the
range of reported data shown in the above table. In addition, Sarma et al. (2018)
reported that the leaves also contained vitamin E in the range of 3.20–3.69 mg/100 g
of the dried leaves and Periyanayagam et al. (2012) reported that leaves contained
11.73% (w/w) ash. The above information proves that the leaves are very nutritive
and contain substantial amount of vitamins and minerals, and therefore, six leaves
with a little bit of slaked lime is said to be comparable to about 300 ml of cow milk
particularly for the vitamin and mineral nutrition (Guha 2006). The calcium content
of betel leaf gets further elevated when slaked lime is added to it as one of the ingre-
dients of a betel quid before consumption in India and other Asian countries. It is
also interesting to note that the leaves contain potassium nitrate ranging from 0.26%
to 0.42% on dry weight basis (CSIR 1969). This may be one of the reasons for use
of betel leaf extracts in food preparations for augmenting sensory qualities of the
native dishes in some countries like India and Bangladesh. This compound is also
supposed to be good for the teeth because it relives toothache due to hypersensitiv-
ity of the damaged or diseased teeth (caries) to a great extent. This information has
been commercially exploited by some toothpaste manufacturing companies who are
marketing toothpaste which soothes the sensitive teeth.
The origin of betel leaf though relates to the antiquity, but essential oil of betel leaf
came into major public domain only when Guenther (1952) published some scien-
tific details of the oil. However, commercialization of essential oil of betel leaf
started in a massive way in India after the recent advent of the betel leaf oil extractor
designed and developed at IIT Kharagpur, India (Guha 2006). Shukla (2015)
reported that about 120 million kg of essential oils are produced globally from
nearly 300 crops which worth about $4 billion, including 4% production from India
amounting to about 21%–22% of the total revenue (Devi et al. 2015). However, no
such data relating to essential oil of betel leaf is available in the public domain till
today, but still, India holds a great promise for production, utilization, and export of
essential oil of betel leaf because the world’s largest quantity and finest quality
(organoleptically superior) leaves are produced in India, particularly in the East and
West Medinipur districts of West Bengal province of India. This promise becomes
more obvious when 10–70% of the leaves are wasted every year in India (Rao and
Narasimham 1977; Guha and Jain 1997; Guha 2006), amounting to a minimum of
₹900 million every year in monetary terms which could be converted into oil, a
measure of generating wealth from waste. In any case, even today, at least 10% of
the total production remains unsold or sold at a throwaway price at any point of
time, and essential oil may be extracted from these surplus leaves (Guha 2006), be
it dried (Hemalatha 2017); fresh, stale, or dechlorophylled; or even partially decayed
and rejected for consumption (Guha 2008). That apart, at least 25% of the leaves are
rejected during curing or bleaching of the leaves in the cottage industries in India.
Moreover, frequently, a large number of the export consignments are destroyed by
incineration mainly due to contamination of Salmonella spp. for avoiding public
health hazards. All these rejected leaves can also be utilized for generating wealth
5 Essential Oil of Betel Leaf (Piper betle L.): A Novel Addition to the World Food… 159
by extraction of essential oil. Such extracted oil would carry the pertinent fragrance
and flavour of the source plants or varieties numbering over 100, but all these oils
could be grouped into six different categories as mentioned previously ranging from
Bangla to Sanchi.
Essential oil from betel leaf may be extracted by the common methods employed
for any other essential oil-bearing crops, such as expression, percolation, macera-
tion, enfleurage, solvent extraction, distillation, supercritical fluid extraction, phy-
tonic extraction (using 1,1,1,2-tetrafluoroethane), etc. Among these methods,
distillation has become more popular due to several inherent advantages, and as a
result, several types of distillation techniques have been attempted, such as hydro-
distillation, steam distillation, microwave-assisted hydro-distillation, ultrasound-
assisted hydro-distillation, vacuum distillation, and so on. Among these,
hydro-distillation has become the most popular method because of its simplicity,
easy repair and maintenance, cheapness, and purity of the extracted oil. Mostly, the
Clevenger’s apparatus is used for extraction of essential oil from betel leaf, but it
takes a long time (3–8 h) (Jantan et al. 1994; Arambewela et al. 2005; Arambewela
et al. 2006; Guha 2010; Periyanayagam et al. 2011; Saxena et al. 2014; Das et al.
2016a; Preethy et al. 2017). This is mainly because of the near-water density of
essential oil of betel leaf (0.958–1.057 g/cc, Gildemeister and Hoffmann 1929)
which makes this oil extremely difficult to separate from water present in the
receiver tube of the Clevenger’s apparatus. In contrast, in cases of other crops, the
oils either float above or sink below the water column facilitating separation and
collection of essential oils from water. That apart, very rapid emulsifying ability of
betel oil with water to form a milky emulsion further makes the process of distilla-
tion very difficult. This essential oil does not separate out easily from the milky
emulsion, and therefore, redistillation may be required. Thus, it takes a long time for
completion of the entire process. Over and above, distillation with the Clevenger’s
apparatus has several inherent drawbacks with respect to essential oil of betel leaf,
such as slow extraction process, poor cooling efficiency, escapement of uncon-
densed oil vapour, etc. (Guha 1998, 1999; Guha 2003).
Therefore, suitable modifications were made in the Clevenger’s apparatus, and
the betel leaf oil extractor (modified Clevenger’s apparatus) was designed, devel-
oped, and patented (Indian Patent number 202600, dated 2.3.2007). The compara-
tive designs of both the above-mentioned apparatuses are shown in Figs. 5.4 and
5.5, respectively, and the material and process flowchart for extraction of essential
oil from betel leaf is shown in Fig. 5.6. This extractor saved time and energy to the
extent of 43.85% and 29.80%, respectively, besides increasing the oil yield by
16.20% compared to the Clevenger’s apparatus. The efficiency of the betel leaf oil
extractor could be further enhanced by insulating the heat-radiating portions of the
apparatus by using cheap, readily available, and efficient insulating materials such
160 P. Guha and S. Nandi
Fig. 5.4 Schematic diagram of Clevenger’s apparatus. (Reproduced from Guha 2010)
as asbestos ropes and also by using cold water (15 °C) as cooling agent. Further, the
fuel requirement can also be reduced substantially if the by-product (i.e. the de-
oiled exhausted leaves) are used as fuel after drying the leaves (Guha 2010). This
would further economize the extraction process particularly in the large installa-
tions in the rural areas. This extractor consumed about 2.1 kWh of electrical energy
in about 2.5 h time for each charge (Guha 2007a, b). Therefore, it is possible to carry
out multiple charging in a single day which will also substantially reduce the cost of
production. Further, when the density of the betel oil obtained from any particular
variety is exactly the same as that of water, then it becomes extremely difficult to
separate out the oil from water. In those cases, 15% saline water may be used to
separate out betel oil from water with the help of a separating funnel (Guha 2010).
5 Essential Oil of Betel Leaf (Piper betle L.): A Novel Addition to the World Food… 161
Fig. 5.5 Schematic diagram of Betel leaf oil extractor. (Reproduced from Guha 2010)
However, this extractor was envisaged to be affordable to the betel leaf growers
since the cost of fabrication of the extractor was calculated to be ₹10,000 and
₹20,000 for 10 and 20 L sizes, respectively (Guha 2007a). This could be easily
maintained by the small farmers and would also be sufficient for processing of sur-
plus leaves in any average-sized Boroj (~0.02 ha) on a daily or weekly basis.
In an attempt to further improve the efficiency of the extraction process and to
increase the yield of essential oil, Amaresh et al. (2017) explored extraction of
essential oil from betel leaves with the help of microwave (2.45 GHz)-assisted
hydro-distillation using modified Clevenger’s apparatus (MAHD). In this attempt,
the extraction process was completed within about 50 minutes compared to about
3.5 h required by the conventional hydro-distillation method using Clevenger’s
apparatus (CHD). The power level and leaf-to-water ratio for maximum oil yield
was 500 W and 0.33 for MAHD, whereas it was 500 W and 0.2 for CHD. The total
power consumed by MAHD was 0.4 kWh, whereas it was 0.7 kWh for CHD. In
both the cases, oil yields were about 1.46% on dry weight basis from Mitha variety
of betel leaf. Thus, there was substantial saving of time (about 76%) and energy
(about 43%) by using MAHD compared to CHD. Moreover, there was no difference
162 P. Guha and S. Nandi
Fig. 5.6 Process and material flow chart for extraction of essential oil from betel leaves of
Ramnagar Mitha variety with the betel leaf oil extractor. (Reproduced from Guha 2010)
in the quality of the essential oil extracted by both the methods, as in both the cases,
15 compounds were detected by GC-MS analysis and the major compounds identi-
fied were 4-allyl-1,2-diacetoxybenzene, caryophyllene, chavibetol, chavicol, and
estragole, whereas anethole, camphene, ả-cardinol, cubenol, eucalyptol, globulol,
linalool, and ô-muurolene were also present in trace amount. The physical proper-
ties of the oils extracted by both the methods were also almost the same except that
the oil extracted by CHD was yellow in colour, whereas it was colourless to yellow
in case of MAHD. Further, the antioxidant activity of both the oils was also almost
5 Essential Oil of Betel Leaf (Piper betle L.): A Novel Addition to the World Food… 163
the same and was not statistically different. Obviously, microwave-assisted irradia-
tion did not interfere with the radical scavenging activity of the extracted essential
oils. This is also confirmed from the fact that the compositions of both the oils were
also the same, and these common constituents contributed to the radical scavenging
activity in the same way.
In another study, to further improve the efficiency of the extraction process and
also to increase the yield of essential oil, Hans (2017) attempted extraction of essen-
tial oil from betel leaves by ultrasound-assisted hydro-distillation (UAHD). This
method involves application of high-intensity and high-frequency sound waves.
Ultrasound is produced by electrical equipment that vibrates with extremely high
frequency. Crystals of some materials such as quartz vibrate very fast when electric-
ity passes through it. As the crystal vibrates, it pushes and pulls air around it produc-
ing ultrasound waves (>20 kHz). This vibration passes through the medium which
is water in this case. As a result, the intermolecular forces are not able to hold the
molecular structure leading to breakdown of the bonds among the water molecules,
and cavitation process takes place. Thus, bubbles are formed and imploded in an
incomprehensively fast manner leading to very high and low pressure points. This
damages the plant cell walls and sucks the contents (including oil) into the sur-
rounding medium and also generates heat. Thus, it facilitates much quicker extrac-
tion of essential oil. In this study, a Toshniwal ultrasound bath (US bath) of 2 L size
with maximum power level of 100 W was used, producing a high-frequency sound
waves of 37 ± 3 kHz. About 200 g of de-petiolated betel leaves were placed in the
US bath for different duration of 30, 60, 90, and 180 minutes with leaf-to-water
ratios of 1:1, 1:2, and 1:3 as a measure of pre-treatment. Subsequently, these leaves
were hydro-distilled to obtain essential oil. The highest oil yield of 0.25% (db) was
obtained with a pre-treatment of 90 minutes and leaf-to-water ratio of 1:2. On the
other hand, the highest oil yield of 0.20% (db) was obtained in all the leaf-to-water
ratios with CHD. However, by decreasing the pre-treatment duration from 90 min-
utes to 60 or 30 minutes, the oil yields were decreased. The subsequent distillation
time, however, was reduced to 1.5 h as compared to 4 h required for CHD due to
ultrasound pre-treatment. This was due to the fact that the ultrasonication damaged
and ruptured the cell structures of the leaves including the epidermal layer which
was confirmed by observation with scanning electron microscope. Such damage
facilitated enhanced migration of the oil micro-globules from the cells to the outside
medium, i.e. water, by the process of cavitation. The CHD consumed 0.67 kWh of
electrical energy, while the UAHD consumed 0.46 kWh for completion of the
extraction process including pre-treatment and subsequent hydro-distillation. This
clearly shows that there was 31% saving of energy in UAHD compared to CHD.
However, UAHD can be proved to be much more advantageous compared to CHD
if the power level of the US bath can be increased beyond 100 W to a suitably higher
level which remains open for further investigation.
It is also possible to employ solvent extraction method for obtaining essential
oil from betel leaf. The different solvents tried were hexane, methanol, ethanol,
acetone, etc. Hexane when used in combination with Soxhlet apparatus and rotary
vacuum evaporator also yielded about the same amount of essential oil as with
164 P. Guha and S. Nandi
CHD, but it contained chlorophyll and all other components that were soluble in
organic solvents, such as wax, fat, vitamin, etc. Therefore, the solvent-extracted
essential oil had a green colour and was not pure in nature, rather a mixture, though
flavour and fragrance were comparable to those extracted by distillation methods.
This method may be costlier than CHD due to inclusion of cost of the solvent.
Therefore, it is not recommended for commercial purpose unless properly
modified.
Crop yield is a very wide term and hence, sometimes becomes confusing unless
defined properly for a particular objective. Yield of essential oil can be defined in
terms of unit oil produced (volume or mass) per ha or oil produced per unit weight
of raw material. The most common unit of expression of essential oil yield is per-
cent of volume of oil per unit mass of raw material (% v/w). However, moisture
percentage of the raw materials plays an important role in calculating yield; hence,
it is expressed as fresh weight basis (wet basis, wb) or dry weight basis (db) and
rarely on air dry basis (adb) of the raw materials. When neither the fresh nor the dry
weights are available, the moisture content of the raw materials is required to be
mentioned along with the oil yield. This is more so because except a few, all the
essential oils are very costly, for example, 1 litre of rose oil may cost as high as ₹5
lakh, and that of pure sandal wood oil may cost more than ₹50 lakh depending upon
the quality of the products. However, db is the most commonly understood form of
expression when it is not mentioned otherwise.
Yield of any crop may take the highest or the lowest value depending upon sev-
eral factors like genetic factor besides soil, climate, and management factors which
interact intricately, sometimes even incomprehensively. Like any other crop, yield,
composition, and quality of essential oil-bearing crops also vary due to several fac-
tors that may also include age of the plant, age of the leaves or plant parts, time of
harvest, method of extraction, process parameters, duration of extraction, nature of
solvent used, pre-treatment of the raw materials, duration of storage of the raw
materials before extraction, etc. However, yield of essential oil of betel leaf gener-
ally varies widely from 0.09% to 0.80% on fresh weight basis (Sankar et al. 1996;
Periyanayagam et al. 2011). This depends mainly on the variety of the leaves and
the local conditions where the vines are grown like any other crop. The local condi-
tions of East and West Medinipur districts of West Bengal province of India are
most suitable for luxuriant growth of betel vines. The world’s highest yield and fin-
est quality leaves are found in these areas. From these areas, leaves of five promi-
nent commercial varieties were collected and then oil was extracted by betel leaf oil
extractor in about 2.5 h (Guha 2007a). The oil yields were 0.8%, 1.7%, 1.7%, 2.0%,
and 2.0% for Sanchi, Sada Bangla, Kali Bangla, Ramnagar Mitha, and Tamluk
Mitha varieties, respectively, on dry weight basis. Sharma et al. (1996) also col-
lected 84 types of betel vines from different parts of India and grew them at National
Botanical Research Institute (NBRI), Lucknow, India. After examination of the
5 Essential Oil of Betel Leaf (Piper betle L.): A Novel Addition to the World Food… 165
quality of the extracted essential oils, these varieties were categorized into five dis-
tinguished flavour groups, namely Mitha, Sanchi, Bangla, Desawari, and Kapoori.
The oil yields of the corresponding groups were approximately 0.25%, 0.18%,
0.16%, 0.14%, and 0.11%, respectively, on fresh weight basis. In another attempt,
from the same institute, NBRI, Lucknow, Rawat et al. (1989) extracted essential oil
from five varieties of betel leaf, namely Mitha, Sanchi, Bangla, Desawari, and
Kapoori and found that the oil yields were 0.85%, 0.19%, 0.16%, 0.12%, and
0.10%, respectively, on fresh weight basis. The yields of both the studies were com-
parable except that of Mitha which may be attributed to the difference in locality
wherefrom the original cuttings were collected for growing the vines. Both the vari-
eties though were named as Mitha, but their exact identity could have been different,
as there are several Mitha varieties mentioned in the literature such as Mitha cum
Bangla, Mitha Calcutta (Sankar et al. 1996), Tamulk Mitha (Guha 2006; Basak and
Guha 2015), Ramnagar Mitha (Guha 2007a), etc.
Das et al. (2016a) extracted essential oil from eight landraces of betel leaf col-
lected from coastal areas of Odisha (India). The essential oil yields varied from
0.10% to 0.42%. The highest yield was obtained from Chandrakala (0.42%) fol-
lowed by Godi Bangla (0.37%), Balia (0.35%), Desibangla (0.32%), Maghai
(0.30%), Dandabalunga (0.20%), Nahua (0.15%), and Karpada local (0.15%).
Surprisingly, contrary to the above, Rayaguru et al. (2007) reported that Godi
Bangla variety from Odisha yielded 9.52% essential oil on dry weight basis, which
is beyond the range found in the contemporary literature and, therefore, needs
confirmation.
Sankar et al. (1996) also collected 13 different cultivars of betel vines from dif-
ferent regions of India and raised them at Guntur, Andhra Pradesh, India. The
mature leaves were used for the extraction of essential oil by the Clevenger’s appa-
ratus, and the oil yields ranged from 0.09% to 0.51% on fresh weight basis. The
minimum oil yield was obtained from Tellaku of Utukur variety (0.09%), whereas
the maximum oil yield was obtained from Mitha Calcutta (0.51%) followed by
Godi Bangla (0.47%), Maghi (0.39%), Mitha cum Bangla (0.33%), Gaach Paan
(0.31%), Kakair (0.26%), Karapaku (0.23%), Pachaikodi (0.20%), Bangla (0.16%),
Kariele (0.18%), Tellaku of Punnur (0.13%), and Tellaku of Chennur (0.11%) vari-
eties, in order.
Preethy et al. (2017) extracted essential oils from five varieties of Indian betel
leaves pertaining to Kerala, namely, Muvattupuzha local, Karinadan, Puthukodi,
Nadan, and Chelan and the corresponding oil yields were 0.57%, 0.52%, 0.50%,
0.47%, and 0.45%, respectively, on fresh weight basis. These yields were positively
correlated to pungency of the leaves which was established from the fact that
Muvattupuzha local with 0.57% oil was the most pungent variety, whereas Chelan
with 0.45% oil was the least pungent variety. In another study, Muhammed (2007)
extracted essential oils from three varieties of betel leaves from Kerala, namely,
Nadan, Selan, and Kuzikkodi, and obtained oil yields of 1% on fresh weight basis in
all the three varieties which was higher than that obtained in the previous study for
the first two varieties and the reason behind it remains open for explanation.
However, oil yields from these three Keralian varieties were comparatively much
higher than those from all other varieties of betel leaf reported so far, except that
166 P. Guha and S. Nandi
reported by Dastane et al. (1958) and Jantan et al. (1994) who obtained 4.20% and
5.10% oil on dry basis, respectively. However, Dastane et al. (op. cit.) reported that
the oil yield increased due to curing treatment of the leaves which is explained in the
foregoing lines.
The CSIR (1969) reviewed oil yields of different varieties of betel leaves, namely,
Calcutta, Gorakhpuri, Saugor, and Ramtek (bleached) and found oil yields as
1.20%, 0.70%, 0.70%, and 2.60%, respectively. It may be noted that oil yield
increased due to curing (bleaching) of the leaves in Ramtek variety. Similar results
were also obtained by Dastane et al. (1958) who obtained 4.20% oil yield from the
bleached leaves in contrast to 1.23% from the fresh leaves of the same variety. Such
increase in oil yield may be explained by the corresponding decrease in other com-
ponents during bleaching such as nonreducing sugar decreased from 1.30% to
0.29%, starch 3.10% to 1.44%, tannin 2.05% to 1.8%, and ether extract 15.7% to
13.5% which cumulatively decreased the total weight of the leaves and, conse-
quently, increased oil yield proportionately compared to the fresh leaves. However,
the phenomenon is not well understood and needs further investigation for elabora-
tion of the results. It is also pertinent to mention here that bleaching of betel leaves
is synonymous with curing. It is a process by which the green leaves of some par-
ticular variety, e.g. Bangla or Maghi, are treated with smoke at moderately high
temperature (36°–45 °C) and pressure (stacking weight loads of baskets full of betel
leaves placed one above the other in rotation). This smoke treatment is given for
30–36 h in a closed chamber and repeated for 4–5 cycles. In absence of light (dark
conditions) and presence of heat and smoke (mainly CO and CO2), the leaves gradu-
ally loose chlorophyll and become light yellow or whitish in colour (Sengupta 1996;
Guha and Jain 1997) known as cured leaves or bleached leaves or Banarasi leaves
with greater organoleptic properties (Guha 2009; Sadhukhan and Guha 2011)
together with a longer shelf life.
The oil yields from other Indian varieties of betel leaf popular in Tamil Nadu
were 0.31% wb (Vellaikodi, Sugumaran et al. 2011), 0.80% adb (Sirugamani-1,
Periyanayagam et al. 2011), and 1.30% adb (Pachaikodi, Vasantha-Srinivasan et al.
2017). Similarly, the oil yields of betel leaf varieties popular in Lucknow area of
Uttar Pradesh province of India, such as Bangladesi and Desawari, were 0.12%
(wb) and 0.15% (wb), respectively (Saxena et al. 2014). Similarly, essential oil
yields of foreign varieties of betel leaves also match with the Indian counterparts,
such as the Philippines (1.44%, Caburian and Osi 2010), Sri Lanka (0.84–1.12% db,
Arambewela et al. 2005, 3.30% w/w adb, Arambewela et al. 2006), and Nepal
(0.10%, Satyal and Setzer 2012), but a report from Malaysia shows a pretty high
value (5.10% db, Jantan et al. 1994). However, one of the earliest reports on essen-
tial oil of betel leaf was published by Guenther during 1958 who reported that the
yields ranged from 0.60% to 1.80%, highest being in the young leaves which are
more pungent than the older leaves. Similar finding was also reported by Pradhan
et al. (2013) and Bhalerao et al. (2013). It may be true that the younger leaves may
contain higher amount of essential oil, but there may be about fivefold (or more)
difference between the fresh weights of the young and fully matured leaves, and
because of this reason, the older leaves are used for extraction of essential oil from
betel leaf on commercial scale.
5 Essential Oil of Betel Leaf (Piper betle L.): A Novel Addition to the World Food… 167
Essential oil of betel leaf is a slightly viscous, greasy, and slippery liquid at room
temperature (Guha 2003). The oil is though colourless immediately after extraction
in most of the cases, but it may vary from faint yellow to yellowish brown (Guenther
1952; Guha 2003). Such difference in colour may be attributed to genetic or varietal,
environmental, edaphic, managerial, processing, and other factors. However, the
colourless oil samples may turn to slightly yellowish after a few hours and dark cof-
fee colour after a few years. It may also turn into dark yellow or orange on exposure
to light or heat (Caburian and Osi 2010), but its aromatic properties remain almost
unaltered during the period of storage. However, the oil possesses a sharp burning
taste (Chakraborty and Shah 2011), burning flavour, and odour reminiscent of creo-
sote and tea (Guenther 1952). On the other hand, Guha (2007a) reported that the oil
extracted from Mitha variety had a pleasant sweet and spicy fragrance, while the
Bangla variety had pungent and spicy fragrance, and the Sanchi variety had the most
intense spicy-pungent odour. Sankar et al. (1996) also reported that fennel
(Foeniculum vulgare Mill)-like aroma and sweet taste in the Mitha Calcutta cultivar
was due to the presence of anethole (54.93%), while the characteristic clove-like
aroma and pungency of the Bangla group of varieties was due to the high concentra-
tion of eugenol (45.30%–57.30%) in the oil. Rawat et al. (1989) also reported that
the essential oil extracted from different varieties of betel leaf was light to dark yel-
low in colour with some kind of spicy fragrance. More specifically, the oil extracted
from Mitha variety was yellowish brown in colour with a fennel-like odour and
sweet taste, while the oil of Kapoori had yellow colour with a greenish tinge and an
aromatic flavour, but the oil of Bangla variety had a clove-like spicy odour and a
sharp pungent taste. However, colour of the essential oil extracted by CHD from
Nepalese variety had a pale yellow colour (Satyal and Setzer 2012), but the oil
extracted from Sirugamani-1 variety had a golden yellow colour with aromatic odour
besides pungent taste and refractive index of 1.505 (Periyanayagam et al. 2011).
168 P. Guha and S. Nandi
Dubey and Tripathi (1987) reported some of the important physical properties of
the oil extracted from unspecified varieties of betel leaf, such as specific gravity
(1.04), refractive index (1.52), acid value (2.50), saponification value (140.25), ester
value (137.75), pH (3.35), and solubility (soluble in organic solvents such as ace-
tone, hexane, benzene, butanol, methanol, and solvent ether). In addition, Guha
(2003) reported that the freezing point of the oil of Sada Bangla variety was very
low and ranged from 0 to −5 °C. Gildemeister and Hoffmann (1929) also studied
the properties of essential oils extracted from a large number of varieties of betel
leaf and found that the specific gravity of the oils varied from 0.958 to 1.057. On the
other hand, Arambewela et al. (2005) reported that the specific gravity of five Sri
Lankan varieties of betel leaf ranged from 1.03 to 1.05 only, but in a subsequent
study, Sugumaran et al. (2011) found that specific gravity of essential oil of
Vellaikodi variety was 1.0010 only. Caburian and Osi (2010) also studied physical
properties of the oil and published the results which are more or less the same as
above. In all the cases, the specific gravity of essential oil of betel leaf is mentioned
to be equal or nearly equal to that of water. However, current literatures lack infor-
mation about emulsifying ability of essential oil of betel leaf except a little that was
provided by Guha (2010) who stated that this oil formed milky emulsion very rap-
idly with water. This phenomenon hinders extraction and separation of oil from
aqueous medium in hydro-distillation or steam distillation process. However, such
striking ability of formation of emulsion may lead to formation of micro- and nano-
emulsions, whereby useful physical, chemical, and biological properties of the oil
may be augmented by many folds, for example, the antimicrobial efficacy of the oil
may be significantly enhanced (Basak and Guha 2017a; Roy and Guha 2018), and
the latter authors along with Basak (2017) are also attempting to enhance bioavail-
ability of the active components of the oil by encapsulation, active packaging, fumi-
gation (vapour phase delivery system), etc., and studies in this direction are in
progress. This is expected to evolve more appropriate and economically viable uti-
lization strategy in future.
Sharma et al. (1996) concluded that the particular flavours of Bangla, Desawari,
Kapoori, Mitha, and Sanchi cultivars could be attributed, respectively, to the pres-
ence of high concentration of phenolics, phenolic ether, and terpenes in combina-
tion with isoeugenol, anethole, and phenolic ethers such as safrole.
5.12 B
iochemical Composition and other relevant details
of Essential Oil of Betel Leaf
It is a widely accepted fact that there are over 100 varieties of betel leaf (Peter 2004;
Guha 2006), but according to Ravindran et al. (2002), it accounts to about 150.
Among them, some varieties have different names in different geographical loca-
tions, for example, Bangla variety is variously known as Godi Bangla, Simurali
Bangla (Ramamurthi and Rani 2012), Calcutta Bangla (Dhongle and Kogje 2013),
5 Essential Oil of Betel Leaf (Piper betle L.): A Novel Addition to the World Food… 169
Sada Bangla, Kali Bangla, Ramtek Bangla, Desi Bangla, Desi Paan, Bangla Deshi
(Saxena et al. 2014), and so on in different parts of India, and their exact identity
remains mostly doubtful. That apart, plants grown asexually from cuttings (for pre-
serving their genetic makeup) also change their morphological and other character-
istics so significantly that they achieve a completely new identity and, hence, known
by different names in different geographical locations with different agro-climatic
conditions. Such changes in the vine can also occur like any other crops merely due
to different management practices, particularly that pertaining to agronomy or hor-
ticulture. Therefore, identification of a particular variety becomes a very difficult
task. In view of that, several workers have tried to identify a specific variety with the
help of biochemical composition of essential oil extracted from the leaves of that
particular variety, treating it as a bio-marker. Some scientists have also tried to iden-
tify the actual active ingredients, i.e. the biochemical compounds responsible for
specific characteristics of any particular variety, such as particular taste or aroma,
antimicrobial activity, insecticidal properties, or medicinal effects. Some scientists
have also tried to examine the toxic effects of any particular variety by detecting
presence of possible harmful biochemical compounds in the plant or in the oil.
Further, in search of commercially valuable biochemical compounds, such as euge-
nol acetate, hydroxychavicol, or chlorogenic acid, some scientists took up studies
on biochemical composition of essential oil of betel leaf with the help of advanced
technologies such as GC-MS, LC-MS, co-GLC, co-TLC, and NMR technologies,
while some others had taken up such studies purely in pursuit of their academic
interest.
It is a matter of common understanding that essential oil is not a pure substance
in the sense that it is not consisted of just one biochemical compound, rather, it is a
mixture of several compounds, which may go up to 300 in betel leaf oil, but it is
generally restricted to 20–30 compounds (Sandra and Bicchi 1987), though 40–50
compounds are also not very infrequent (Basak and Guha 2015). However, if merely
any one of these compounds is removed partly or totally, then the oil may not retain
its original aroma or other characteristics. All these organic compounds can be
grouped into about nine classes for the sake of convenience (Table 5.4), such as
monoterpenes, sesquiterpenes, alcohols, aldehydes, acids, oxides, phenols, phenolic
ether, esters, besides others like ketone as reported by Das et al. (2016a). Examples
of some of the biochemical compounds pertaining to essential oil of betel leaf clus-
tered in these groups are also shown in Table 5.4. Additionally, the organoleptic,
functional, and biological properties of some of the important compounds are given
in Table 5.5, while relevant synonyms are given in Table 5.6, and the molecular
structures of some of the prominent compounds with molecular formula and molec-
ular weight are shown in Fig. 5.7.
The earliest report on the examination of essential oil of betel leaf by Guenther
(1952) showed that chemical composition of betel oil varied with origin of the leaf and
the oils contained up to 55% phenols, mostly chavibetol and sometimes chavicol.
Garg and Jain (1996) identified 21 constituents in the essential oil extracted from
Sagar Bangla cultivar which was rich in chavicol (48%).
Table 5.4 Classification of biochemical compounds present in the essential oil of betel leaf
170
S. Phenolic
no. Monoterpenes Sesquiterpenes Alcohols Aldehydes Acids Oxides Phenols ethers Esters
1 α-Thujeneb,c γ-Cadineneb,c Linaloolb,c Decanal (capric Hexadeconoic 1,8-cineolb,c Eugenolb,c,e Methyl Eugenol
aldehyde)b,c acidb,c eugenolb,c,e acetateb,c
2 Campheneb,c Δ-cadineneb,c,e α-Terpineolb,c Decanal (laural Terpinyl Caryophyllene Iso-eugenolb,c Methyl Methyl
aldehyde)b,c acetatec oxideb,c chavicolb benzoateb
3 Sabineneb,c α-Cadineneb,c Terpinol-1-olb,c Stearaldehydeb,c Chlorogenic Chavicolb,c,e Anetholeb,c Methyl
acidd salicylatee
4 Trans-sabinene β-Salineneb,c α-Costolb n-Decanole Chavibetolb,c,e Safroleb,c Chavibetol
hydratee acetatee
5 β-Myrceneb,c β-Slemeneb,c,f Δ-Cardinolb,c Hydroxy- Allylpyrocatecol
chavicola diacetatee
6 Trans-β- γ-Elemeneb,c 3,7,11,15-tetra-
ocimeneb,c methyl-2-
hexadecane-olb
7 Bornyleneb,c Cis- Geraniolb,c
caryophylleneb,c,e
8 β-Pineneb,c Trans- α-Cardinole
Caryophylleneb,c,e
9 Trans-β- Aromadendreneb,c τ-Muurolole
ocimeneb,c
10 γ-Terpenineb,c α-Cubebeneb,c α-Selinenolf
11 Terpinoleneb,c β-Cubebeneb,c
12 Allo-ocimeneb,c α-Humulenee
13 α-Terpeneneb,c γ-Muurolenee
14 β-Phellandreneb,c Germacrene Df
15 Limoneneb,c Lepidozenef
P. Guha and S. Nandi
16 p-cymeneb,c
17 2,6,6-Trimethyl-
1-methyl-cyclo-
hex-2-eneb
18 α-Pineneb,c
a
Amonkar et al. (1986)
b
Rawat et al. (1989)
c
Sharma et al. (1996)
d
Guha (2006)
e
Satyal and Setzer (2012)
f
Periyanayagam et al. (2011)
5 Essential Oil of Betel Leaf (Piper betle L.): A Novel Addition to the World Food…
171
172 P. Guha and S. Nandi
Table 5.5 Organoleptic, functional, and biological properties of major components of essential oil
of betel leaf
S. Name of the Organoleptic Functional and biological
no. compounds properties properties References
1 Eugenol Pleasant, Anti-inflammatory, Rai et al. (2011); Foo
spicy, antimicrobial, analgesic, et al. (2015); Das et al.
clove-like antioxidant, antiviral, (2016b); Kudva et al.
odour anti-carcinogenic, (2018); HMDB (2018c)
antidepressant, antiseptic,
anaesthetic in dentistry,
anti-mutagenic, insecticidal,
fungicides
2 Chavibetol Spicy odour Noncentral analgesic, NCBI (2018); Das et al.
anti-pyretic, anti- (2016b)
inflammatory, antibacterial,
antineoplastic,
antiperspirants
3 Hydroxy- Light smell of Anti-carcinogenic, Nagabhushan et al.
Chavicol creosote anti-nitrosation, (1989); Sharma et al.
antimutagenic, anti- (2009); Rathee et al.
inflammatory, antioxidant, (2006); Chakraborty et al.
antibacterial, antiplatelet, (2012); Vikash et al.
antithrombotic, xanthine (2012); Bhalerao et al.
oxidase inhibitory, and (2013); Kumar et al.
gastric ulcer-healing activity (2015); Abdullah et al.
(2016); Singh et al.
(2018)
4 β-Caryophyllene Spiciness of Anti-inflammatory, Calleja et al. (2013);
black pepper, anti-carcinogenic, pain Cheng et al. (2014);
woody-spicy, relief, primary therapeutic Mahmoud et al. (2014);
dry, clove-like against atherosclerosis and Bahi et al. (2014);Chang
aroma osteoporosis, prevents et al. (2013); Gertsch
diabetes, endometriosis et al. (2008)
cerebral ischaemia, anxiety
and depression, liver
fibrosis, and Alzheimer-like
disease
5 Methyl eugenol Clove-like Used in aroma therapy and GOC (2010); Joshi
aroma massage oil, anti- (2013); Das et al. (2016b)
inflammatory, cytotoxic
against human cell line,
insecticidal activity, used as
fragrance ingredients in
perfumes, toiletries, and
detergents
6 Cubebene Citrus type Anti-inflammatory, Lee et al. (2012); HMDB
antiseptic, antioxidant, (2018b); Zahin et al.
immunomodulatory (2018)
activities, neuroprotection
against glutamate-induced
oxidative injury, used as
surfactant and emulsifier
(continued)
5 Essential Oil of Betel Leaf (Piper betle L.): A Novel Addition to the World Food… 173
Table 5.5 (continued)
S. Name of the Organoleptic Functional and biological
no. compounds properties properties References
7 Estragole Odour Antimutagenic, antifungal EU (2001); Chang et al.
reminiscent of against some bacteria, (2009); Zielińska and
anise, sweet flavouring agent, food Matkowski (2014); PF
taste additive (2018)
8 Anethole Sweet Antimicrobial, antifungal, Balasubramanyam and
anise-like anthelmintic, insecticidal, Rawat (1990); Marinov
flavour, 13 anti-inflammatory, and Valcheva-Kuzmanova
times sweeter antinociceptive, (2015)
than sugar gastroprotective and
anti-implantation,
secretolytic, expectorant,
spasmolytic, estrogenic,
sedative, flavouring agent in
food industry
9 Iso-eugenol Floral odour Antimicrobial, antioxidant, Atsumi et al. (2005);
reminiscent of anti-inflammatory, Khan (2014); Hyldgaard
carnation flavouring agent et al. (2015)
innonalcoholic drinks,
baked foods, and chewing
gums
10 Safrole Spicy odour Anti-inflammatory, Nagabhushan et al.
detoxifying agent, (1989); Parise-Filho et al.
antioxidant, antimicrobial, (2011); Da Silveira et al.
antimutagenic, (2014); Madrid et al.
immunosuppressive, (2014); Das et al.
beverage and candy (2016b); Marko (2017)
preparation but carcinogenic
to rat (not to humans)
11 α-Copaene Woody flavour Antimicrobial, Brito et al. (2005)
antiproliferative,
antioxidant, antigenotoxic,
and anticytotoxic activities
12 Chavicol Phenolic Antimicrobial, antioxidant, Nagori et al. (2011);
odour antiseptic Murakami et al. (2015);
HMDB (2018a)
Thahn et al. (2002) described a new chemotype of betel leaf from Hue area of
Vietnam that contained isoeugenol (72%) as the chief component which was fol-
lowed by isoeugenol acetate (12%). Therefore, the variety may be named isoeuge-
nol chemotype of Piper betle.
Sharma et al. (1981) examined essential oil extracted from Kapoori variety of
betel leaf and found that terpinyl acetate (21.98%) and eugenol (15.83%) were the
prominent ingredients.
Sugumaran et al. (2011) examined essential oil obtained from Vellaikodi variety
of betel leaf which is popular in Tamil Nadu (India) and identified a total of 65 com-
pounds. The major compound was safrole (25.67%) which was followed by eugenol
174 P. Guha and S. Nandi
Table 5.6 Names and synonyms of prominent compounds found in the essential oil of betel leaf
S. Name of the CAS
no. compounds no. Synonyms
1 Eugenol 97-53-
4-Allyl-2-methoxyphenol; p-Allylguaiacol; p-eugenol;
0 eugenic acid
2 Eugenyl acetate 93-28-
Acetyleugenol; 4-Allyl-2-methoxyphenyl acetate; phenol,
7 2-methoxy-4-(2-propenyl)-, acetate; Aceto eugenol;
1-Acetoxy-2-methoxy-4-allylbenzene
3 Chavibetol 501- m-eugenol; 5-Allyl-2-methoxyphenol;
19-9 3-Allyl-6-methoxyphenol
4 Hydroxy-chavicol 1126- 4-Allylpyrocatechol, 4-Allylbenzene-1,2-diol;
61-0 4-Allylcatechol; Desmethyleugenol
5 β-Caryophyllene 87-44- 8-Methylene-4,11,11-(trimethyl)bicyclo (7.2.0) undec-4-ene
5
6 Methyl eugenol 93-15- Eugenol methyl ether; 4-Allyl-1,2-dimethoxybenzene;
2 benzene, 1,2-dimethoxy-4-(2-propenyl)-
7 α-Cubebene 1769- 3,7-Dimethyl-4-(propan-2-yl)-3a,3b,4,5,6,7-
14-8 hexahydro-1 h-cyclopenta[1,3]cyclopropa[1,2]benzene
8 Estragole 140- 4-Allylanisole, methyl chavicol; tarragon; anisole, p-allyl-;
67-0 Chavicol, O-methyl-; p-Allylanisole; p-Methoxyallylbenzene
9 Anethole 4180- 4-Propenylanisole, Isoestragole, (E)-1-Methoxy-4-(1-
23-8 propenyl)benzene
10 Iso-eugenol 97-54- 2-Methoxy-4-propenylphenol, 4-Propenylguaiacol;
1 2-Methoxy-4-(1-propenyl)phenol
11 Safrole 94-59- 4 allyl 1,2 methylenedioxybenzene; 1,3-Benzodioxole,
7 5-(2-propenyl)-; Shikomol; 4-Allylpyrocatechol
formaldehyde acetal
12 α-Copaene 3856- Copaene; Tricyclo[4.4.0.02,7]dec-3-ene,1,3-dimethyl-8-(1-
25-5 methylethyl)-, stereoisomer
13 Chavicol 501- 4-Allylphenol; p-Hydroxyallylbenzene;
92-8 4-(2-propenyl)-phenol
14 Isoxylic acid 610- Benzoic acid, 2,5-dimethyl-; 2-Carboxy-1,4-
72-0 dimethylbenzene; 2,5-Dimethylbenzoic acid
15 β-Sitosterol 83-46- Cupreol; Quebrachol; α-Dihydrofucosterol; β-Sitosterin;
5 Angelicin; Triastonal; 5-Cholesten-24β-ethyl-3β-ol
(18.27%) and eugenol acetate (8.00%). Another report from Mysore (India) also
showed high safrole content (39.74%) in the oil obtained from a local variety
(Ramalakshmi et al. 2002).
Basak and Guha (2015) characterized the chemical compounds present in Tamluk
Mitha variety of East Medinipur district of West Bengal (India) and identified 46
different compounds, among which prominent compounds were chavibetol
(22.00%), estragole (15.80%), β-cubebene (13.60%), chavicol (11.80%), and caryo-
phyllene (11.30%). The compositional study showed that the natural sweetening
compound anethole was totally absent in this variety. Therefore, it may be possible
that estragole contributed (Table 5.5) the peculiar sweet aroma and taste to this vari-
5 Essential Oil of Betel Leaf (Piper betle L.): A Novel Addition to the World Food… 175
Fig. 5.7 Molecular structure, formula and weight of major compounds of essential oil of different
varieties of betel leaf
176 P. Guha and S. Nandi
ety. Rawat et al. (1989) found that essential oil of Mitha variety contained anethole
(19.31%) and cis-caryophyllene (10.64%), whereas the oil of Sanchi variety con-
tained stearaldehyde (2.69%) which was not present in any other variety examined
together, namely, Bangla, Mitha, Desawari, and Kapoori. Therefore, presence of
stearaldehyde may be used as a biomarker for identification of Sanchi cultivar.
Mohottalage et al. (2007) reported that Sri Lankan betel leaf oil contained safrole
(52.70%) as the major constituent which was followed by allylpyrocatechol diace-
tate (15.40%), eugenol (6.40%) and eugenol acetate (5.85%).
Prakash et al. (2010) identified 32 compounds from essential oil of Maghi variety
of betel leaf from Varanasi (India). The major constituent was eugenol (63.39%)
which was followed by acetyl eugenol (14.05%), while the other components cumu-
latively contributed less than three percent.
Karak et al. (2018) analysed essential oils from seven varieties of betel leaf from
West Bengal, India. They identified 45 constituents in total, which included 14
monoterpenes, 23 sesquiterpenes, and 8 phenyl propanes. In Bangla, Bagerhati,
Manikdanga, and Ghanagate varieties, the prominent components were eugenol
acetate (31.46–43.97%) and eugenol (13.13–33.06%), whereas in Mitha variety, it
was chavicol (23.85%), while in Chhaanchi variety, safrole (42.77%) was the chief
ingredient. In absence of a large proportion of anethole in Mitha variety, the sweet
fragrance was probably contributed by the presence of estragole (Basak and Guha
2015) since it is also reported to possess similar organoleptic characteristics
(Table 5.5).
Sugumaran et al. (2011) extracted essential oil from Sirugamani variety of betel
leaf and found 67 compounds, among which the major compound was safrole
(32.79%) followed by eugenol (16.17%), eugenol acetate (8.01%), τ-gurjunene
(4.14%), and sabinene (3.43%). On the other hand, Periyanayagam et al. (2011)
extracted essential oil from a similarly named betel leaf variety of Tamil Nadu
(India), i.e. Sirugamani-1, and found that the oil was constituted of 59 biochemical
compounds. The major compound was germacrene-D (16.07%) followed by lepi-
dozene (14.99%), β-caryophyllene (9.86%), 1,3,4-eugenol (7.17%), β-elemene
(5.75%), γ-murrolene (3.18%), α-seleninol (3.07%), β-cadinene (2.82%), and cineol
(2.80%). By comparing the composition of the above two varieties, it may be con-
cluded that similarity in the varietal names does not ensure similarity in composition
of essential oils of the varieties. This also indicates that the nomenclature of the betel
leaf varieties has not been scientifically accomplished. Therefore, research work in
this area becomes essential for proper identification of the varieties of betel leaf.
Saxena et al. (2014) identified 25 and 35 components in the essential oils of
Bangladeshi and Desawari varieties of betel leaf representing only 85.40% and
86.11% of the oil, respectively. The prominent components identified were eugenol,
α-celinene, α- farnesene, p-celinene, methyl eugenol, and germacrene-D in both the
varieties, but safrole and isosafrole were present only in Desawari variety. Safrole is
commonly thought to be a potential carcinogenic agent, but that is a misconception.
This is because safrole is quickly metabolized in human body into di-hydroxychavicol
and eugenol, which are excreted along with urine (Chang et al. 2002).
5 Essential Oil of Betel Leaf (Piper betle L.): A Novel Addition to the World Food… 177
Preethy et al. (2017) extracted essential oils from five varieties of betel leaves
available in Malappuram district of Kerala (India). They identified a maximum of
56 compounds in total. In all the varieties, hydroxychavicol (39.50–45.50%) was
the principal compound followed by eugenol (11.00–20.80%) though other com-
pounds like methyl isoeugenol (0.10%–1.50%), methyl eugenol (0–0.80%), and
isoeugenol (0.80–1.00%) were also present. The names of the varieties, percentage
of hydroxychavicol, and number of compounds therein were as follows: Karinadan
(45.50%, 39), Pathukodi (45.30%, 55), Nadan (44.60%, 56), Muvattupuzha local
(41.10%, 55), and Chelan (39.50%, 56).
Das et al. (2016a) studied essential oils of eight landraces of betel leaf collected
from coastal areas of Odisha (India) and identified 50 compounds in total, among
which eugenol was the chief ingredient in all the landraces; however, its percentage
varied, such as in Chandrakala (34.61%), Karpada local (39.84%), Godibangla
(44.04%), Nahua (44.96%), Balia (45.85%), Desibangla (46.47%), Dandabalunga
(55.49%), and Maghai (71.87%). From this work of Das et al. (op. cit.), it may be
concluded that all these varieties possess clove-like pungency which increased with
increasing proportion of eugenol in these varieties. Therefore, these results may
serve as an excellent example of increasing order of organoleptic properties, i.e.
clove-like pleasant pungency, which may be introduced in different products requir-
ing appropriate amount of pungency. For example, a new product, a lozenge, for
soothing throat problems (Vijay 2015), for infants should have minimum pungency,
but for the elderly, the maximum amount of pungency may be introduced. This is
because senses in the infants are very acute, whereas it becomes gradually blunt
with age in the elderly.
Sankar et al. (1996) examined essential oil from 13 cultivars of betel vines of
Andhra Pradesh (India) and found that in most of the varieties, eugenol (45.33–
57.39%) was the chief ingredient present in different proportions as shown in paren-
theses of the foregoing lines. The varieties included Karapaku (57.39%), Pachaikodi
(56.09%), Bangla (54.66%), Kariele (50.89%), Maghai (50.77%), Kakair (47.20%),
Gaachi Paan (47.13%), Mitha cum Bangla (45.33%), and Godi Bangla (45.10%).
However, in some varieties, anethole (54.93%) was the chief ingredient as in Mitha
Calcutta variety, wherein proportion of eugenol (20.27%) was the second most abun-
dant component next to anethole. On the other hand, terpinyl acetate, in different
proportions, as shown in the parentheses, was the chief ingredient in some other
varieties, such as Tellaku of Pannur (48.76%), Tellaku of Utukur (37.43%), and
Tellaku of Chennur (34.21%). However, these three varieties also contained eugenol
to the extent of 17.00%, 23.16%, and 18.42%, respectively. In all these 13 varieties,
methyl eugenol and α-terpineol were also present in the proportions ranging from
5.11% to 0.07%. Based on the chemical constituents of betel leaf oils, all these 13
cultivars can be classified into four groups, namely, Bangla, Mitha, Sanchi, and
Kapoori. However, this grouping is not complete, since there are some overlapping.
Muhammed (2007) extracted essential oil from three varieties of betel leaf of
Keralian (India) origin, namely, Nadan, Kuzhikkodi, and Selan, and identified 40,
43, and 38 compounds, respectively. The major compound in Nadan variety was
178 P. Guha and S. Nandi
Table 5.7 Classification of different varieties of betel leaf into the major chemotypic groups
S. Chemotypic Name of variety and percentage of chief
no. group components References
1 Chavicol Sagar Bangla (48%) Garg and Jain (1996)
Mitha (23.85%) Karak et al. (2018)
2 Germacrene-D Sirugamani-1 (16.07%) Periyanayagam et al.
(2011)
3 Isoeugenol Vietnamese variety (72%) Thahn et al. (2002)
4 Chavibetol Betel leaf from the Philippines (53.10%) Rimando et al. (1986)
Malaysian variety (69.0%) Jantan et al. (1994)
Nepal variety (80.50%) Satyal and Setzer (2012)
5 Eugenol Bangla (63.56%) Rawat et al. (1989)
Kapoori (33.22%)
6 Anethole Mitha (19.30%) Rawat et al. (1989)
7 Safrole Sri Lankan variety (52.70%) Mohottalage et al.
(2007)
Taiwanese (inflorescence) varieties (28%) Dwivedi and Tripathi
(2014)
Desawari (45.34%) Rawat et al. (1989)
Sanchi (22.75%)
Chhaanchi or Sanchi (42.77%) Karak et al. (2018)
8 Eugenol acetate Kali Bangla (22.16%) Karak et al. (2018)
Manikdanga (44.03%)
Bangla (35.77%)
Ghanagete (43.97%)
Bagerhati (31.46%)
This edible leaf has achieved an esteemed position in human society right from the
dawn of civilization, particularly in most of the Asian countries (Khoshoo 1981;
Samanta 1994; Jana 1996; Sharma et al. 1996; Guha 2006), and also in different
other countries of the world among the Asian immigrants. The leaves are tradition-
ally used for chewing in their natural raw condition along with many taste-enhancing
ingredients like sliced areca nuts, Kattha (thick paste of wood extract of Acacia
catechu L.), slaked lime, etc., for obtaining mainly refreshing, stimulating, mood-
elevating, digestive, and aphrodisiac effects (CSIR 1969; Garg and Jain 1996; Guha
1997, 2006, Chu 2001). These beneficial effects may be attributed mainly to the
essential oil present in the leaves (Guha 1997; Khanra 1997) which is constituted of
a large number of biochemical compounds with distinct bioactivity (Pradhan et al.
2013; Basak and Guha 2015; Roy and Guha 2018). Such useful properties of the oil
indicate a promising industrial future for manufacturing of a large number of cos-
metics, medicines, pharmaceuticals (Guha 1997, 2000, 2002), insecticides (Tabacchi
180 P. Guha and S. Nandi
and Guerin 2007; Vasantha-srinivasan et al. 2017), fungicides (Ansari et al. 2017),
food preservatives (Basak 2018a, b, c; Basak and Guha 2017a), food flavouring
agents (Roy and Guha 2015), and food products, such as paan masala (spiced and
processed betel leaf), cold drinks, gutkha (non-tobacco-based chewable mouth
freshener), chocolates (Guha 1997, 2000), suji halwa (Bhagath and Guha 2014),
chili bo (Wendy et al. 2014), cupcake (Roy and Guha 2015), and many other novel
products.
The distinct bioactivities of essential oil of betel leaf which are most relevant to
the food sector are antioxidant and antimicrobial activities which are discussed in
the foregoing paragraphs.
The antioxidants in relation to food are the substances which minimize, delay, or
prevent oxidation of the target molecules (Halliwell and Gutteridge 1995; Halliwell
2007) like lipids, proteins, nucleic acids, and polysaccharides (Duan and Kasper
2011). The concentration of the antioxidants is normally very low compared to that
of the target molecules. The examples of common (nonenzymatic) antioxidants are
quercetin, vitamin E (α-tocopherol), vitamin C, β-carotene, lycopene, lutein, sele-
nium, polyphenols, carotenoids, etc.
Oxidation of components of food changes the physical, biochemical, nutritional,
and organoleptic properties of the food articles and make it unacceptable and, hence,
renders it unfit for human consumption. Oxidation is caused by the free radicals,
and it is one of the most prominent ways of spoilage of food that reduces the shelf
life of the food articles. The free radicals are ions, atoms, or molecules with unpaired
electrons which make them highly reactive and thereby unstable. These radicals
may originate from elements like oxygen, nitrogen, and sulphur, which produce
reactive oxygen species (ROS), reactive nitrogen species (RNS), and reactive sul-
phur species (RSS), respectively. Free radicals have a variety of chemical mecha-
nisms such as electron donation, reduction of radicals, electron acceptance,
oxidation of radicals, hydrogen abstraction, addition reaction, self-annihilation, and
so on (Slater 1984). These mechanisms are disrupted by nonenzymatic antioxidants,
while the antioxidant enzymes (e.g. catalase) destroy the free radicals by various
other ways like metabolization, neutralization, catalytic break down, etc. in pres-
ence of some co-factors like copper, zinc, manganese, etc. When these mechanisms
are disrupted or the free radicals are destroyed, shelf life of the food articles is
enhanced. For these purposes, synthetic antioxidants such as butylated hydroxytolu-
ene (BHT), butylated hydroxyanisole (BHA), propyl gallate (PG), and tert-
butylhydroxyquinone (TBHQ) are used in a large scale in the commercial food
products. Unfortunately, all these synthetic antioxidants have one or more adverse
effects (Kahl and Kappus 1993; Lorenzo et al. 2018), many of which have not been
explored or disclosed in order to protect commercial interest. Therefore, some other
potential natural antioxidants like essential oils are being explored as an alternative
5 Essential Oil of Betel Leaf (Piper betle L.): A Novel Addition to the World Food… 181
to the synthetic chemicals. Such antioxidant properties are also reported with respect
to essential oil of betel leaf (Arambewela et al. 2006; Suppakul et al. 2006; Prakash
et al. 2010; Das et al. 2016a). Several components of the leaf and its essential oil,
such as vitamin C, vitamin A, vitamin E, terpenes and its derivatives, phenols and
its derivatives, etc., are reported to be the active ingredients contributing to such
antioxidant property (Guha 2006; Rathee et al. 2006; Swapna et al. 2012; Nagababu
et al. 2014; Bhargava et al. 2015; Chauhan et al. 2016; Chitnis 2017; Sarma et al.
2018). Therefore, it may be concluded that there is a general agreement among the
scientists that betel leaf and its oil possess strong antioxidant activity. Consequently,
several workers have attempted to explore antioxidant capability of the essential oil
of betel leaf for extending shelf life of several food products like apple juice (Basak
2018a), tomato paste (Basak 2018b), ghee (Gupta and Guha 2018), and several
other products. That apart, manufacturing of some novel products can be taken up
like food supplements and pharmaceuticals for treatment of cancer (Shukla et al.
2018; Kudva et al. 2018).
Another distinct bioactivity of essential oil of betel leaf, which is also very much
relevant to the food sector, is the antimicrobial activity which is discussed in the
foregoing paragraph.
We need food for mitigating hunger and for obtaining nutrition, and also for various
other purposes like treatment of illness, convalescence, fun, pass time, social enter-
tainment of guest, etc. Therefore, continuous supply of food is needed, for which it
has to be stored for a long time that requires enhancement of shelf life. However, the
microbes pose a serious threat to this. They not only spoil the food items but also
release microbial toxins into the food which cause several types of diseases and
even death. Therefore, attempts have been made to stop or minimize the harmful
microbial growth to an acceptable limit in food by various means including incor-
poration of antimicrobial agents in the food or in the packaging materials. Some of
the most common synthetic antimicrobial agents and their permissible limits are as
follows: benomyl (<100 μg/kg/day body weight, EHC 1993; Malhotra et al. 2015),
benzoic acid (<1000 ppm, FSSAI 2011), chlorotetracycline (<5 ppm, Sivasankar
2003), imazalil (<61 μg/kg/day, EPA 2005; Malhotra et al. 2015), natamycin
(<500 ppm, HMDB 2011), nisin (<12.5 ppm, FSSAI 2011), oxytetracycline
(<5 ppm, Sivasankar 2003), paraben (<10 ppm of body weight per day, EFSA 2004;
Anand and Sati 2013), etc. These and many more antimicrobial agents are used dur-
ing production of primary food or its processing, packaging, and storage to make
the food articles safe for human consumption. These compounds though effective
may pose several side effects ranging from simple ailments to incurable chronic
diseases like cancer (EPA 2005; HMDB 2011; Anand and Sati 2013) which ulti-
mately leads to painful unnatural death. That apart, many of the synthetic preserva-
tives have pollutive effect and undesirably long persistence conducive to development
182 P. Guha and S. Nandi
Prakash et al. (2010) examined efficacy of essential oil of betel leaf against 1651
fungal isolates belonging to 14 species including six species of Aspergillus and
found that the minimum inhibitory concentration (MIC) was as low as 0.70 μl/
ml and aflatoxin production was completely inhibited at a concentration of
0.60 μl/ml.
5 Essential Oil of Betel Leaf (Piper betle L.): A Novel Addition to the World Food… 183
Dubey and Tripathi (1987) found that the oil was toxic at a very low concentra-
tion of 0.20–0.50 μl/ml to 13 fungi including Alternaria, Aspergillus, Botryodiplodia
(syn. Lasiodiplodia), Penicillium, and Rhizopus species. In a similar study, Garg
and Jain (1992) reported that the essential oil of betel leaf (var. Sagar Bangla) was
effective against nine strains of fungi including five strains of Aspergillus along with
A. niger at a very low concentration (5 μl/ml). Apart from being effective against
fungi, the oil was also found by them to be effective against three strains of gram-
positive bacteria (Bacillus subtilis, B. pumilus, and Staphylococcus aureus) and two
strains of gram-negative bacteria (Salmonella typhi and Vibrio cholerae). In further
studies, the oil was also found to be toxic to Penicillium expansum in the experi-
ments conducted by Basak and Guha (2015) who estimated the Emax (minimum
concentration of oil at which mold growth was inhibited) and MIC values to be in
the order of 0.56 and 0.74 μl/ml in both the potato dextrose agar medium (PDA) and
apple juice agar medium. In continuation of this study, Basak (2018c) also found
that essential oil of betel leaf (var. Tamluk Mitha) inhibited germination of both A.
flavus and P. expansum at very low concentrations of 0.65 and 0.54 μl/ml, respec-
tively, in PDA.
Apart from the Indian reports as above, similar reports are also available from
foreign countries. Suppakul et al. (2006) found that essential oil of betel leaf from
Thailand was highly effective against 13 strains of microorganisms including 5
strains of gram-positive bacteria, 5 strains of gram-negative bacteria, and 3 strains
of yeast at a very low concentration (12.50–100 μl/ml) except Pseudomonas aeru-
ginosa which was not sensitive to this oil even at the highest concentration (200 μl/
ml). The susceptible microorganisms included Bacillus cereus, Escherichia coli,
Salmonella enteritidis, and Staphylococcus aureus besides Candida albicans and
Saccharomyces cerevisiae. It is, however, suggested that anti-quorum sensing prop-
erties of the active ingredients of essential oil may be explored for reducing the viru-
lency of P. aeruginosa (Umar et al. 2018) which is reported to be resistant to this oil
(Suppakul et al. 2006).
Caburian and Osi (2010) also explored the essential oil of Filipino betel leaf and
concluded that the oil was effective against four harmful microorganisms. The mini-
mum inhibitory concentrations were 250.00, 125.00, 15.60, and 1.95 μg/ml for
Candida albicans, Staphylococcus aureus, Streptococcus pyogenes, and
Trichophyton mentagrophytes, respectively.
Satyal and Setzer (2012) experimented with chavicol-rich Nepalese betel oil and
found that several microorganisms were susceptible to the oil at a very low concen-
tration (MIC values shown in parentheses as μg/ml) such as Candida albicans
(1250), Escherichia coli (625), Pseudomonas aeruginosa (625), Staphylococcus
aureus (625), and Aspergillus niger (313). In another study from India on yeast,
Rath and Mohapatra (2015) also found that essential oil of betel leaf was highly
effective against four strains of Candida species at a very low concentration and the
MIC ranged from 31.25 to 125.00 μl/ml.
184 P. Guha and S. Nandi
Roy and Guha (2018) reported further that the antimicrobial efficacy of the oil
(var. Tamluk Mitha) was enhanced by formulating a nano-emulsion with Tween 20
dispersed in water (3% oil, 3–6% Tween 20, and rest water) compared to macro-
emulsion against three strains of gram-negative bacteria, namely, Escherichia coli,
Klebsiella pneumonia, and Pseudomonas aeruginosa. For nano-emulsion, the MIC
ranged from 0.50 to 1.00 μl/ml, whereas for macro-emulsion, it was 0.75–1.25 μl/
ml. Similarly, for nano-emulsion, the minimum bactericidal concentration ranged
from 1 to 2 μl/ml, whereas for macro-emulsion, it was 1.50–2.50 μl/ml. In another
study on two strains of fungi (Aspergillus flavus and Penicillium expansum), Basak
and Guha (2017b) concluded that micro-emulsion of essential oil of betel leaf (var.
Tamluk Mitha) was highly toxic at a lower concentration (0.40 μl/ml), whereas
sporicidal activity was found at a relatively higher concentration (15 μl/ml).
Basak and Guha (2017b) studied the ultramicroscopic structures of the treated
spores and found that the oil affected the membrane integrity of the spores causing
release of the internal cellular materials. Vintilla (2018) also reported modification
of permeability or integrity of the cytoplasm or cell structures. That apart, there
were cytoplasmic coagulation, shrinkage, granulation, and serious morphological
damages. Several other authors also observed the destruction of cytoplasmic mem-
brane leading to coagulation of cellular materials and other serious damages (Pauli
2001; Suppakul et al. 2006; Rath and Mohapatra 2015; Roy and Guha 2018). It is
clear from these studies that the mode of antimicrobial action is similar in all the
cases irrespective of type of cells and microorganisms, such as fungal hyphae,
spores, both gram-positive and gram-negative bacterial cells, and even the budded
cells of the yeasts. In all the cases, detrimental effects, deformation, and death are
caused which is reported to take just a minute in case of Candida (Rath and
Mohapatra 2015), but the time required for other species needs to be investigated
and quantified. The active ingredients responsible for such detrimental effects are
reported to be the derivatives of terpenes and phenols such as carvacrol, caryophyl-
lene, chavibetol, eugenol, limonene, methyl eugenol, pinene, safrole, and some ste-
rols (α-cardinol, linalool, β-sitosterol, etc.), which are present in the essential oil of
betel leaf in different proportions, such as chavicol (0.40–48.00%), eugenol (6.40–
63.56%), safrole (22.75–52.70%), etc. depending upon the varieties and environ-
mental, edaphic, and managerial factors.
There are only a few reports available in the current literature for the confirma-
tion of efficacy and mode of action of the supposed active ingredients discussed
above. Therefore, the active ingredients in pure form and its systematic dilutions
should be experimented with different strains of microorganisms separately. That
apart, studies on the combination of active ingredients should also be taken up for
exploring their synergistic, additive, or antagonistic effects. Such experiments will
generate relevant information conducive for commercial exploitation of antimicro-
bial formulations from natural sources like betel leaf in future.
5 Essential Oil of Betel Leaf (Piper betle L.): A Novel Addition to the World Food… 185
Betel leaf is intended for human consumption due to its nutritional, organoleptic,
and several other useful qualities. Therefore, it comes within the domain of food.
The essential oil contained in the leaves has multiple unique natural characteristics
relevant to the food industries, such as unique aroma and taste besides antimicrobial,
antioxidant, and many other characteristics of high therapeutic significance. These
characteristics provide a challenging opportunity to the food scientists and tech-
nologists to develop novel food products with enhanced food safety, extended shelf
life, attractive sensory, therapeutic, prophylactic, functional, and other desirable
qualities. However, research work in this domain is very limited. Therefore, research
work was taken up at IIT, Kharagpur, and a few novel products have been developed
with incorporation of essential oil of betel leaf ranging from 0.005% to 0.50% of the
main ingredients. These developed products include ice cream (Jain 2012), choco-
late (Godbole 2013), suji halwa (Bhagath and Guha 2014), cupcake (Roy and Guha
2015), lozenge (Vijay 2015), rosogolla, i.e. sweet balls of milk solids (Rajput 2017),
etc. These products are not only novel but also expected to include most of the ben-
eficial effects of the oil including the digestive one contributing to better health
benefits to the consumers. That apart, the textural and organoleptic properties of all
these developed products were also found to be better than or comparable to the
bestselling commercial products available in the market. Such product development
is envisaged to be economic, self-sustaining, and profitable to the entrepreneurs.
This is also envisaged to minimize the wastage of surplus leaves which may range
from 10% to 70% of the total annual production (Rao and Narasimham 1977; Guha
and Jain 1997; Guha 2006) mainly during the glut season and thereby increase prof-
itability of the betel vine farmers and the traders as well (Guha 2014).
Roy and Guha (2015) manufactured a novel cupcake with common ingredients
using essential oil of betel leaf as a novel food additive (0.005% v/w). The textural,
sensory, and economic data indicated that the betel-flavoured cupcake was compa-
rable to the bestselling commercial cupcakes available in the market. Similar is the
case with biscuit (Maurya 2014). This is the first time that two bakery products have
been developed with essential oil of betel leaf and that such entrepreneurship could
be profitable and self-sustaining.
Basak (2018b) reported that essential oil of betel leaf (var. Tamluk Mitha) has an
enormous potential as a natural preservative in the food sector due to its safety and
antimicrobial effectivity at a very low concentration (0.25 mg/g in micro-emulsion
form) without adversely affecting sensory qualities of the food products. At this
concentration, shelf life of the product (tomato paste) could be extended by 14 more
days compared to control in an accelerated storage study (temperature 39 °C ± 1 °C
and relative humidity 89% ± 1.2%). Therefore, it is envisaged that this oil has a
tremendous future potential in the food processing industries dealing with tomato
and its preparations. Similarly, this oil has also a tremendous future potential in
another food processing industry dealing with apple and its products, particularly
the apple juice which is a highly demanded nutritional drink all over the world.
This industry is under a great threat of a bio-toxin called Patulin produced by the
186 P. Guha and S. Nandi
Acknowledgments The authors are grateful to the present and the former Directors of IIT
Kharagpur, Prof. Partha Pratim Chakrabarty and Prof. Sishir Kumar Dubey, respectively, for kind
encouragement, funds, and facilities for this work. They are also highly grateful to Prof. V. K.
Tewari, and Prof. N. Mallick, former and current Heads of the Department of Agricultural and
Food Engineering, IIT Kharagpur, India. Thanks are also due to Prof. P. P. Srivastav, Prof. S. L.
Shrivastava, Prof. K. N. Tiwari, and Prof. Rintu Banerjee of the same department. Thanks are also
due to Mr. Arnab Roy, Ms. Mitali Madhumita, and Mr. Jagan S. Karthik, the research scholars
working with the senior author, and to Mr. Anas Ejaz Shaikh (M. Tech. student of the same depart-
ment) for their help in preparation of this manuscript. The authors are grateful to Dr. Yoshisuke
Kishida, President and Editor in Chief of the journal, Agricultural Mechanization in Asia, Africa,
and Latin America, and also to the Editor in Chief of the Journal of Human Ecology, New Delhi,
for providing permission to reproduce small portions from the previously published papers con-
tributed by the senior author. The authors are also grateful to Dr. P. C. Singh, NBRI, Lucknow,
India for providing literature on anticancer plants. The authors are also grateful to Prof. Sonia
Malik, Federal University of Maranhão, Sao Luis, Brazil, for her encouragement without which
this work would have not seen the light of the day. The second author is grateful to his family
members for providing continuous support. The last but not the least, the senior author is grateful
to his better half, Srimati Chhaya Guha, for her constant inspiration, encouragement, and
sacrifice.
References
Abdullah NF, Mohamad Hussain R, Amom Z (2016) Effect of APC on killing of Staphylococcus
aureus by oxidative stress agents. J Teknol 78(5-5):39–44
Agarwal T, Singh R, Shukla AD et al (2012) Comparative analysis of antibacterial activity of four
Piper betle varieties. Adv Appl Sci Res 3(2):698–705
Amaresh A, Guha P, Khan S et al (2017) Comparative study of microwave assisted hydro-
distillation with conventional hydro-distillation for extraction of essential oil from Piper betle
L. Biosci Biotechnol Res Asia 14(1):401–407
Amonkar AJ, Nagabhushan M, D’Souza AV et al (1986) Hydroxychavicol: a new phenolic antimu-
tagen from betel leaf. Food Chem Toxicol 24(12):1321–1324
Anand SP, Sati N (2013) Artificial preservatives and their harmful effects: looking towards nature
for safer alternatives. Int J Pharmaceu Sci Res 4(7):2496–2501
Ansari MJ, Al-ghamdi A, Usmani S et al (2017) In vitro evaluation of the effects of some plant
essential oils on Ascosphaera apis, the causative agent of Chalkbrood disease. Saudi J Biol Sci
24:1001–1006
Arambewela L, Kumaratunga KGA, Dias K (2005) Studies on Piper betle of Sri Lanka. J Natl Sci
Found Sri Lanka 33(2):133–139
Arambewela L, Arawwawala M, Rajapaksa D (2006) Piper betle: a potential natural antioxidant.
Int J Food Sci Technol 41(1):10–14
Atsumi T, Fujisawa S, Tonosaki K (2005) A comparative study of the antioxidant/pro-oxidant
activities of eugenol and isoeugenol with various concentrations and oxidation conditions.
Toxicol In Vitr 19(8):1025–1033
Bahi A, Mansouri SA, Al Memari E et al (2014) β-caryophyllene, a CB2 receptor agonist pro-
duces multiple behavioral changes relevant to anxiety and depression in mice. Physiol Behav
135:119–124
Balasubramanyam VR, Rawat AKS (1990) Studies on morphology and chemistry of Piper betle
L. J Plantation Crops 18:78–87
Barnett C (2015) Making perfume from the rain. Available at: https://www.theatlantic.com/inter-
national/archive/2015/04/making-perfume-from-the-rain/391011/. Retrieved on 01/07/2018
188 P. Guha and S. Nandi
Basak S (2017) Antifungal efficacy of essential oil of betel leaf (Piper betle L.) on Aspergillus
flavus and Penicillium expansum and its potential as food preservative. PhD thesis (Under the
guidance of Prof. P. Guha), Agricultural and Food Engineering Department, Indian Institute of
Technology, Kharagpur (W. B.), India
Basak S (2018a) The use of fuzzy logic to determine the concentration of betel leaf essential oil
and its potency as a juice preservative. Food Chem 240:1113–1120
Basak S (2018b) Shelf life extension of tomato paste through organoleptically acceptable con-
centration of betel leaf essential oil under accelerated storage environment. J Food Sci
83(5):1396–1403
Basak S (2018c) Modelling the effect of betel leaf essential oil on germination time of Aspergillus
flavus and Penicillium expansum spore population. LWT-Food Sci Technol 95:361–366
Basak S, Guha P (2015) Modelling the effect of essential oil of betel leaf (Piper betle L.) on ger-
mination, growth, and apparent lag time of Penicillium expansum on semi-synthetic media. Int
J Food Microbiol 215:171–178
Basak S, Guha P (2017a) Betel leaf (Piper betle L.) essential oil microemulsion: characterization
and antifungal activity on growth, and apparent lag time of Aspergillus flavus in tomato paste.
LWT-Food Sci Technol 75:616–623
Basak S, Guha P (2017b) Use of predictive model to describe sporicidal and cell viability efficacy
of betel leaf (Piper betle L.) essential oil on Aspergillus flavus and Penicillium expansum and
its antifungal activity in raw apple juice. LWT-Food Sci Technol 80:510–516
Bevilacqua A, Speranza B, Perricone M et al (2018) Bioactivity of essential oils towards fungi
and bacteria: mode of action and mathematical tools. In: Hashemi SMB, Khaneghah AM, de
Sant’Ana AS (eds) Essential oils in food processing: chemistry, safety and application. Wiley,
New York
Bhagath BVVB, Guha P (2014) Development of novel Sooji Halwa with unique properties of
essential oil of betel leaf. Int J Agric Food Sci Technol 5:87–93
Bhalerao SA, Verma DR, Gavankar RV et al (2013) Phytochemistry, pharmacological profile and
therapeutic uses of Piper betle Linn. – an overview. J Pharmacogn Phytochem 1(2):10–19
Bhargava P, Uppoor AS, Pralhad S et al (2015) An in-vitro study of determination of anti-bacterial,
antioxidant, anti-inflammatory potential of Piper betle essential oil. Natl J Integr Res Med
6(2):37–44
Böhme K, Barros-Velázquez J, Calo-Mata P et al (2014) Antibacterial, antiviral and antifungal
activity of essential oils: mechanisms and applications. In: Villa TG, Veiga-Crespo P (eds)
Antimicrobial compounds. Springer, Berlin, pp 51–81
Brito MVH, de Moreira RJ, Tavares MLC et al (2005) Copaiba oil effect on urea and creatinine
serum levels in rats submitted to kidney ischemia and reperfusion syndrome. Acta Cir Bras
20(3):243–246
Burdock GA, Carabin IG (2004) Generally recognized as safe (GRAS): history and description.
Toxicol Lett 150(1):3–18
Burkill IH (1966) A dictionary of economic products of the Malaysian peninsula, Vol. II. Ministry
of Agriculture, Malaysia, pp 1767–1772 (Reprint)
Burt S (2004) Essential oils: their antibacterial properties and potential applications in foods – a
review. Int J Food Microbiol 94:223–253
Caburian AB, Osi MO (2010) Characterization and evaluation of antimicrobial activity of the
essential oil from the leaves of Piper betle L. E-Intl Sci Res J 2(1):2–13
Calleja MA, Vieites JM, Montero-Meterdez T et al (2013) The antioxidant effect of β-caryophyllene
protects rat liver from carbon tetrachloride-induced fibrosis by inhibiting hepatic stellate cell
activation. Brit J Nutr 109(3):394–401
de Candolle A (1884) Origin of cultivated plants. Kegan P, London (Quoted from Handbook of
Herbs and Spices Vol. 2 edited by Peter KV, CRC Press)
Chakraborty D, Shah B (2011) Antimicrobial, anti-oxidative and anti-hemolytic activity of Piper
betle leaf extracts. International J Pharm Pharmaceu Sci 3:192–198
Chakraborty JB, Mahato SK, Joshi K et al (2012) Hydroxychavicol, a Piper betle leaf component,
induces apoptosis of CML cells through mitochondrial reactive oxygen species-dependent
5 Essential Oil of Betel Leaf (Piper betle L.): A Novel Addition to the World Food… 189
JNK and endothelial nitric oxide synthase activation and overrides imatinib resistance. Cancer
Sci 103(1):88–99
Chang MC, Uang BJ, Wu HL et al (2002) Inducing the cell cycle arrest and apoptosis of oral
KB carcinoma cells by hydroxychavicol: roles of glutathione and reactive oxygen species. Br
J Pharmacol 135:619–630
Chang CL, Cho IK, Li QX (2009) Insecticidal activity of basil oil, trans-anethole, estragole, and
linalool to adult fruit flies of Ceratitis capitata, Bactrocera dorsalis, and Bactrocera cucurbi-
tae. J Econ Entomol 102:203–209
Chang HJ, Kim JM, Lee JC et al (2013) Protective effect of β-caryophyllene, a natural bicyclic
sesquiterpenes, against cerebral ischemic injury. J Med Food 16(6):471–480
Chattopadhyay SB, Maity S (1967) Diseases of betel vine and spices. ICAR, New Delhi
Chauhan ES, Aishwarya J, Singh A et al (2016) A review: nutraceuticals properties of Piper betle
(Paan). Am J Phytomedicine Clin Ther 4(2):28–41
Cheng Y, Dong Z, Liu S (2014) β-Caryophyllene ameliorates the Alzheimer-like phenotype in
APP/PS1 mice through CB2 receptor activation and the PPARγ pathway. Pharmacology
94(1–2):1–12
Chitnis KS (2017) Quantitation of eugenol in betel leaf varieties by HPTLC. Int J Pharm Sci Res
8(11):4858–4862
Chu NS (2001) Effects of betel chewing on the central and autonomic nervous systems. J Biomed
Sci 8(3):229–236
CSIR (Council of Scientific and Industrial Research, New Delhi) (1969) The Wealth of India.
CSIR, New Delhi, pp 84–94
Da Silveira ESRDC, Andrade LN, De Oliveira RDRB et al (2014) A review on anti-inflammatory
activity of phenylpropanoids found in essential oils. Molecules 19(2):1459–1480
Das S, Parida R, Sandeep IS et al (2016a) Chemical composition and antioxidant activity of some
important betel vine landraces. Biologia 71(2):128–132
Das S, Parida R, Sriram IS et al (2016b) Biotechnological intervention in betel vine (Piper betle
L.): a review on recent advances and future prospects. Asian Pac J Trop Med 9(10):938–946
Dastane NG, Patil RG, Chaugule BA (1958) This business of bleaching betel leaves. Indian
Farming 7(10):10–12
Devi MP, Chakrabarty S, Ghosh SK et al (2015) Essntial oil: its economic aspect, extraction,
importance, uses, hazards and quality. In: Sharangi AB, Datta S (eds) Value addition of horti-
cultural crops: recent trends and future directions. Springer, India, pp 269–273
Dhongle SS, Kogje KK (2013) Comparative pharmacognostic study of leaves of two varieties
of Piper betle L. namely Calcutta Bangla and Assam Kapoori. Int J Herb Med 1(3):115–119
Duan J, Kasper DL (2011) Oxidative depolymerization of polysaccharides by reactive oxygen/
nitrogen species. Glycobiology 21(4):401–409
Dubey P, Tripathi SC (1987) Studies on antifungal, physico-chemical and phytotoxic properties of
the essential oil of Piper betle. Dis Prot 94(3):235–241
Dwivedi V, Tripathi S (2014) Review study on potential activity of Piper betle L. J Pharmacogn
Phytochem 93(34):93–98
EFSA (2004) EFSA advises on the safety of paraben usage in food. Available at: https://www.efsa.
europa.eu/en/press/news/040929. Retrieved on 27/08/2018
EHC (1993) Environmental Health Criteria 148, Benomyl. Available at: http://www.inchem.org/
documents/ehc/ehc/ehc148.htm. Retrieved on 27/08/2018
Ekka A (2015) Development of novel toothpaste with essential oil of betel leaf. B. Tech the-
sis (under the supervision of Prof. P. Guha), Agricultural and Food Engineering Department,
Indian Institute of Technology, Kharagpur (W. B.), India
EPA (US Environmental Protection Agency) (2005) US EPA archive document, R.E.D Facts.
Imazalil, pp 1–8
EU (European Commission) (2001) Opinion of the scientific committee on food on estragole
(1-allyl-4-methoxybenzene). SCF/CS/FLAV/FLAVOUR/6 ADD2 FINAL. Available at:
https://ec.europa.eu/food/sites/food/files/safety/docs/fs_food-improvement-agents_flavour-
ings-out104.pdf. Retrieved on 01/08/2018
190 P. Guha and S. Nandi
Ferreres F, Oliveira AP, Gil-Izquierdo A et al (2014) Piper betle leaves: profiling pheno-
lic compounds by HPLC/DAD-ESI/MS and anti-cholinesterase activity. Phytochem Anal
25(5):453–460
Foo LW, Salleh E, Nur S et al (2015) Extraction and qualitative analysis of Piper betle leaves for
antimicrobial activities. Int J Eng Technol Sci Res 2:1–8
FSSAI (2011) Food Safety and Standards (Food Product Standards and Food Additives) Regulation,
2011. Available at: http://fsdaup.gov.in/writereaddata/images/pdf/act-and-rules/fss-regulation/
Food-safety-and-standards-Food-product-standards-and-Food-Additives-regulation-2011.pdf.
Retrieved on 27/08/2018
Garg SC, Jain R (1992) Biological activity of the essential oil of Piper betle L. J Essent Oil Res
4(6):601–606
Garg SC, Jain R (1996) Chavicol rich essential oil of Piper betle L., cultivar Sagar Bangla. Euro
Cosmet 5:27–28
Gertsch J, Leonti M, Raduner S et al (2008) β-caryophyllene is a dietary cannabinoid. Proc Natl
Acad Sci 105(26):9099–9104
Gildemeister E, Hoffmann F (1929) Die Atherischen Ole, vol Vol. II, 3rd edn. Wiley, New York,
p 474
GOC (Government of Canada) (2010) Risk management scope for Benzene, 1,2-dimethoxy-4-(2-
propenyl)-methyl eugenol. Chemical Abstract Service Registry Number (CAS RN): 93-15-2.
Environment Canada Health. Available at: http://www.ec.gc.ca/substances/ese/eng/challenge/
batch9/batch9_93-15-2_rm_en.pdf. Retrieved on 19/07/2018
Godbole AH (2012) Development of novel shampoo with essential oil of betel leaf. B. Tech the-
sis (under the supervision of Prof. P. Guha), Agricultural and Food Engineering Department,
Indian Institute of Technology, Kharagpur (W. B.), India
Godbole AH (2013) Development of novel chocolate with unique properties of essential oil of
betel leaf (Piper betle L.). M. Tech thesis (under the supervision of Prof. P. Guha), Agricultural
and Food Engineering Department, Indian Institute of Technology, Kharagpur (W. B.), India
Gopalan C, Rama SBV, Balasubramanian SC (1984) Nutritive value of Indian food. National
Institute of Nutrition, ICMR, Bangalore, India, p 108
Guenther E (1948) The essential oils, vol I. D. Van Nostrand & Co., Inc., New York
Guenther E (1952) The essential oils, 5th edn. D. Van Nostrand & Co, Inc., New York, pp 160–161
Guha P (1997) “Paan Theke Kutir Silpa Sambhabana” (In Bengali). “Exploring Betel Leaves for
cottage industry”, pp. 15–19, In: Krishi, Khadya-O- Gramin Bikash Mela – a booklet pub-
lished by the Agricultural and Food Engineering Department, Indian Institute of Technology,
Kharagpur, India
Guha P (1998) Extraction of essential oil from betel leaves cultivated in and around Medinipur dis-
trict. In: Annual report of All India Coordinated Research Project on Postharvest Technology
(ICAR). Indian Institute of Technology, Kharagpur, pp 53–58
Guha P (1999) Extraction of essential oil from betel leaves. In: Annual report of All India
Coordinated Research Project on Postharvest Technology (ICAR). Indian Institute of
Technology, Kharagpur, pp 41–57
Guha P (2000) Commercial exploitation of oil from betel leaves. In Proc. 6th Regional workshop
on oil seeds and oils, held at IIT, Kharagpur, West Bengal, India on 26th February, 2000,
pp 56-57
Guha P (2002) Essential oil extraction from betel leaves: A promising options for rural employ-
ment. In: National Symposium on Information Technology for Sustainable Agriculture held
during Jan 28–30, 2002 at Agricultural and Food Engineering Department, Indian Institute of
Technology, Kharagpur, pp 174–175
Guha P (2003) Extraction of essential oil from betel leaves grown in and around Medinipur dis-
trict. In: Annual report of All India Coordinated Research Project on Postharvest Technology
(ICAR). Indian Institute of Technology, Kharagpur, pp 15–23
Guha P (2006) Betel leaf: the neglected green gold of India. J Hum Ecol 19(2):87–93
Guha P (2007a) Extraction of essential oil: an appropriate rural technology for minimizing wast-
age of surplus betel leaves. Agric Mech Asia, Africa Lat Am 38(4):47–50
5 Essential Oil of Betel Leaf (Piper betle L.): A Novel Addition to the World Food… 191
Guha P (2007b) Extraction of essential oil from betel leaf for manufacturing of food, medicinal, and
cosmetics products. In: Proceedings of Indian Convention of Food Scientist and Technologists
held during 31/12/2007 to 02/01/2008 at Indian Institute of Technology, Kharagpur, p 44
Guha P (2008) Processing, packaging and marketing of betel leaf (Piper betle L.). In national
seminar on Piperaceae-harnessing agro-technologies for accelerated production of economi-
cally important Piper species, IISR, Calicut, Kerala, India, pp 258–269
Guha P (2009) Postharvest physiology of cured betel leaf. In: National Conference on Frontiers in
Plant Physiology. Jorhat, Assam, India, p 25
Guha P (2010) Design, development, testing and comparative evaluation of the betel leaf oil
extractor. Agric Mech Asia, Africa Lat Am 41(4):28–35
Guha P (2012) Employment generation through cultivation and processing of betel leaves in
Chhattisgarh. In: National Seminar on role of Science and Technology for sustainable develop-
ment of Chhattisgarh, Bhilai, India, p 01
Guha P (2014) Postharvest management of betel leaf (Piper betle L.): a review. In: Compendium
of national meet on betel vine held at IIHR, Bangalore, India, pp 72–75
Guha P, Jain RK (1997) Status report on production, processing and marketing of betel leaf (Piper
betle L.). Agricultural and Food Engineering Department, Indian Institute of Technology,
Kharagpur, India
Gupta N, Guha P (2018) Efficacy of essential oil of betel leaf (Piper betle L.) essential oil as a nat-
ural antioxidant for ghee preservation. International conference on Recent Advances in Food
Processing Technology held at Indian Institute of Food Processing Technology, Thanjavur,
Tamil Nadu, India, during 17–19th August, 2018. Poster Presentation. Poster code- C163
Gupta D, Singh A (2016) Piper betle and some Indian plant for antidepressant activity: a review.
Res J Pharm Biol Chem Sci 7(2):1670–1678
Gutierrez J, Bourke P, Lonchamp J et al (2009) Impact of plant essential oils on microbiological,
organoleptic and quality markers of minimally processed vegetables. Innov Food Sci Emerg
Technol 10(2):195–202
Halliwell B (2007) Biochemistry of oxidative stress. Biochem Soc Trans 35:1147–1150
Halliwell B, Gutteridge JM (1995) The definition and measurement of antioxidants in biological
systems. Free Radic Biol Med 18:125–126
Hans N (2017) Comparison of ultrasound assisted and conventional methods of extraction of
essential oil from betel leaf (Piper betle L.). M. Tech thesis (under the supervision of Prof.
P. Guha), Agricultural and Food Engineering Department, Indian Institute of Technology,
Kharagpur (W.B.), India
Hemalatha DH (2017) Microwave assisted extraction of essential oil from dried betel leaves
(Piper betle L.). B. Tech thesis (under the supervision of Prof. P. Guha), Agricultural and Food
Engineering Department, Indian Institute of Technology, Kharagpur (W.B.), India
HMDB (The Human Metabolome Database) (2011) Natamycin, Material Safety Data Sheet
Version 2.0 (Revised on 28/09/2011). Available at: http://www.hmdb.ca/system/metabolites/
msds/000/013/627/original/Natamycin_MSDS.pdf?1368650610. Retrieved on 27/08/2018
HMDB (The Human Metabolome Database) (2018a). Chavicol. Available at: http://www.hmdb.
ca/metabolites/HMDB0034107. Retrieved on 28/07/2018
HMDB (The Human Metabolome Database) (2018b). Cubebene. Available at: http://www.hmdb.
ca/metabolites/HMDB0036413. Retrieved on 28/07/2018
HMDB (The Human Metabolome Database) (2018c). Eugenol. Available at: http://www.hmdb.ca/
metabolites/HMDB0005809. Retrieved on 28/07/2018
Hyldgaard M, Mygind T, Piotrowska R et al (2015) Isoeugenol has a non-disruptive detergent-like
mechanism of action. Front Microbiol 6:1–14
Islam QMS, Martin MA, Rashid MA et al (2015) Profitability of betel leaf (Piper betle L.) cultiva-
tion in some selected sites of Bangladesh. Bangladesh J Agric Res 40:409–420
Jain G (2012) Development of Novel Ice-cream with unique properties of essential oil of betel leaf
(Piper betle L.). M. Tech thesis (under the supervision of Prof. P. Guha), Agricultural and Food
Engineering Department, Indian Institute of Technology, Kharagpur (W.B.), India
Jana BL (1995) Gram Banglar Arthakari Phasal-Paan (In Bengali). Betel leaf: a cash crop of
villages of Bengal. Asaboni, Flat 203, 184, B B Chatterji Road, Calcutta, West Bengal, India.
192 P. Guha and S. Nandi
Jana BL (1996) Improved technology for betel leaf cultivation. A paper presented in the Seminar-cum-
Workshop on betel leaf marketing held at state cashew nut farm, Directorate of Agricultural
Marketing, Digha, Midnapur (W.B.), India, June 5–6, 1996
Janaiah K (2017) Development of a novel Kathi Goja fortified with discarded petioles of betel
leaf industry. M. Tech thesis (under the supervision of Prof. P. Guha), Agricultural and Food
Engineering Department, Indian Institute of Technology, Kharagpur (W.B.), India
Jantan I, Ahmad AR, Ahamd AS et al (1994) Comparative study of the essential oils of five Piper
species from Peninsular Malaysia. Flavour Fragr J 9:339–342
Jeng JH, Chen SY, Liao CH et al (2002) Modulation of platelet aggregation by areca nut and betel
leaf ingredients: roles of reactive oxygen species and cyclooxygenase. Free Radic Biol Med
32(9):860–871
Joshi RK (2013) Chemical composition, in-vitro antimicrobial and antioxidant activities of the
essential oils of Ocimum gratissimum, O. sanctum and their major constituents. Indian J Pharm
Sci 75(4):457–462
Kahl R, Kappus H (1993) Toxicology of the synthetic antioxidants BHA and BHT in comparison
with the natural antioxidant vitamin E. Z Lebensm Unters Forsch 196:329–338
Karak S, Acharya J, Begum S et al (2018) Essential oil of Piper betle L. leaves: chemical com-
position, anti-acetylcholinesterase, anti-β-glucuronidase and cytotoxic properties. J Appl Res
Med Aromat Plants. Available at: https://doi.org/10.1016/j.jarmap.2018.06.006. Retrieved on
08/09/2018
Khan TH (2014) Isoeugenol accelerates wound healing by attenuating pro-inflammatory markers
and chemokine expression in diabetic mice. In: 5th World Congress on Bioavailability and
Bioequivalence held at DoubleTree by Hilton Baltimore-BWI Airport, USA from September
29 to October 01, 2014, p 4172.
Khanra S (1997) Paan Vittik Silpakendra (In Bengali). “Betel leaf based industry”. Nabanna
Bharati 30(2):169
Khoshoo TN (1981) Welcome address, pp. xvii–xx. In: Proc of group discussion on Improvement
of Betel Vine Cultivation. Khanduja SD, Balasubrahmanyam VR (eds.). National Botanical
Research Institute, Lucknow, India
Kraft P (2004) Aroma Chemicals (4th ed): Musks. In: Rowe DJ (ed) Chemistry and technology of
flavours and fragrances. Blackwell, UK, pp 143–145
Kudva AK, Rao S, Rao P et al (2018) Piper betle Linn. in cancer: past, present and future. In:
Akhtar MS, Swamy MK (eds) Anticancer plants: properties and application, vol 1. Springer
Singapore Pte Ltd., pp 327–348
Kumar S, Pathania AS, Satti NK et al (2015) Synthetic modification of hydroxychavicol by
Mannich reaction and alkyne-azide cycloaddition derivatives depicting cytotoxic potential. Eur
J Med Chem 92:236–245
Laji S (2015) Development of novel medicinal biscuit incorporated with betel leaf powder (Piper
betle L.). M. Tech thesis (under the supervision of Prof. P. Guha), Agricultural and Food
Engineering Department, Indian Institute of Technology, Kharagpur (W.B.), India
Lee SK, Kim SD, Lee HY et al (2012) Iso-cubebene, a natural compound isolated from Schisandra
chinensis fruit, has therapeutic benefit against polymicrobial sepsis. Biochem Biophys Res
Commun 426(2):226–231
Liewen MB, Bullerman LB (1992) Toxigenic fungi and fungal toxins. In: Vanderzant C,
Splittstoesser DF (eds) Compendium of methods for the microbiological examination of foods.
American Public Health Association, Washington, DC, pp 811–819
Lohra S (2015) Development of novel soap with essential oil of betel leaf. B. Tech thesis (under the
supervision of Prof. P. Guha), Agricultural and Food Engineering Department, Indian Institute
of Technology, Kharagpur (W.B.), India
Lorenzo JM, Pateiro M, Domínguez R et al (2018) Berries extracts as natural antioxidants in meat
products: a review. Food Res Int 106:1095–1104
Madrid A, Espinoza L, Pavéz C et al (2014) Antioxidant and toxicity activity in vitro of twelve
safrole derivatives. J Chil Chem Soc 59(3):2598–2601
5 Essential Oil of Betel Leaf (Piper betle L.): A Novel Addition to the World Food… 193
Mahmoud MF, S-El S, Hasan RA et al (2014) Role of cannabinoid receptors in hepatic fibrosis and
apoptosis associated with bile duct ligation in rats. Eur J Pharmacol 742:118–124
Maity S (1989) Extension bulletin: The Betel Vine. All India Coordinated Research Project on
betel vine. Indian Institute of Horticultural Research, Hessarghatta, Bangalore, India
Malhotra B, Keshwani A, Kharkwal H (2015) Antimicrobial food packaging: potential and pitfalls.
Front Microbiol 6:1–9
Marinov V, Valcheva-Kuzmanova S (2015) Review on the pharmacological activities of anethole.
Scr Sci Pharm 2(2):14–19
Marko K (2017) Sassafras reduces inflammation, pain, toxins and more. Available at: https://www.
thealternativedaily.com/sassafras-reduces-inflammation-and-pain/. Retrieved on 21/07/2018
Maurya S (2014) Development of novel biscuit with unique properties of essential oil of betel leaf
(Piper betle L.). M. Tech thesis (under the supervision of Prof. P. Guha), Agricultural and Food
Engineering Department, Indian Institute of Technology, Kharagpur (W.B.), India
Mazumder S, Roychowdhury A, Banerjee S (2016) An overview of betel leaf (Piper betle L.): A
review. Ann Food Sci Technol 17(2):367–376
MHMC (Malaysian Herbal Monograph Committee) (2015) Malaysian Herbal Monograph, Sireh
Leaves. Institute of Medical Research, Ministry of Health, Malaysia, pp 416–427
Mohanto S, Datta S, Mandal S (2017) Piper betle Linn: a brief study. Int J Curr Med Pharm Res
3(2):1290–1296
Mohottalage S, Tabacchi R, Guerin PM (2007) Components from Sri Lanka Piper betle L. leaf
oil and their analogues showing toxicity against the housefly Musca domestica. Flavour Fragr
J 22:130–138
Muhammed AM (2007) Isolation, structure elucidation and properties of secondary metabolites in
plants. PhD thesis (under the guidance of Prof. P. Mohamed Shafi), Department of Chemistry,
University of Calicut, Kerala, India
Murakami Y, Kawata A, Ito S et al (2015) The radical scavenging activity and cytotoxicity of res-
veratrol, orcinol and 4-allylphenol and their inhibitory effects on Cox-2 Gene expression and
Νf-ĸb activation in RAW264. 7 cells stimulated with Porphyromonas gingivalis-fimbriae. In
Vivo 29:341–350
Nagababu E, Rifikind JM, Boindala S et al (2014) Assessment of antioxidant activity of eugenol
in vivo and in vitro. In: Rao MU, Subramanyam NM, William AP, Narasimham LP (eds) Free
radicals and antioxidant protocol. Humana Press, New York, pp 1–5
Nagabhushan M, Amonkar AJ, Nair UJ, D'Souza AV, Bhide SV (1989) Hydroxychavicol: a new
anti-nitrosating phenolic compound from betel vine leaf. Mutagenesis 4:200–204
Nagori K, Singh MK, Alexander A et al (2011) Piper betle L.: a review on its ethnobotany, phy-
tochemistry, pharmacological profile and profiling by new hyphenated technique DART-MS
(Direct Analysis in Real Time Mass Spectrometry). J Pharm Res 4(9):2991–2997
Nalkar P (2015) Development of novel herbal Tea from processed betel leaf. M. Tech thesis (under
the supervision of Prof. P. Guha), Agricultural and Food Engineering Department, Indian
Institute of Technology, Kharagpur (W.B.), India
NCBI (National Center for Biotechnology Information) (2018). Chavibetol. Available at: https://
alsaha.ru/C10H12O2.pdf. Retrieved on 24/07/2018
Nouri L, Nafchi AM (2014) Antibacterial, mechanical, and barrier properties of sago starch film
incorporated with betel leaves extract. Int J Biol Macromol 66:254–259
Nouri L, Nafchi AM, Karim AA (2015) Mechanical and sensory evaluation of noodles incorpo-
rated with betel leaf extract. Int J Food Eng 11(2):221–227
Parise-Filho R, Pastrello M, Pereira CCE et al (2011) The anti-inflammatory activity of dillapiole
and some semisynthetic analogues. Pharm Biol 49(11):1173–1179
Pauli A (2001) Antimicrobial properties of essential oil constituents. Int J Aromather
11(3):126–131
Periyanayagam K, Dhanalakshmi S, Karthikeyan V et al (2011) Phytochemical studies and GC/
MS analysis on the isolated essential oil from the leaves of Piper betle L. var. Sirugamani1
(SGM1). J Nat Prod Plant Resour 4(7):2411–2413
194 P. Guha and S. Nandi
Sadhukhan S, Guha P (2011) Post-harvest technology of betel leaf (Piper betle L.): effect of curing
on quality and physiological parameters. Agric Mech Asia Africa Lat Am 42(3):47–51
Samanta C (1994) Paan chaser samasyabali-o-samadhan: Ekti samikkha (In Bengali): A report
on the problems and solutions of betel vine cultivation. A booklet published by Adhikari HR,
C-2/16, Karunamoyee, Salt Lake City, Kolkata-64 (W.B.), India
Sandra P, Bicchi C (1987) Capillary gas chromatography in essential oil analysis. Alfred Huethig
Verlag, Heidelberg/Basel/New York
Sankar CR, Sridevi D, Babu MK (1996) Studies on essential oil and oil constituents of betel vine
cultivars. Andhra Agric J 43(1):24–26
Sarma C, Kumari S, Rasane P et al (2018) Exploring nutraceutical potential of selected varieties of
Piper betle L. Carpathian J Food Sci Technol 10(1):64–73
Satyal P, Setzer WN (2012) Chemical composition and biological activities of Nepalese Piper
betle L. Int J Prof Holist Aromather 1(2):23–26
Saxena M, Khare NK, Saxena P et al (2014) Antimicrobial activity and chemical composition of
leaf oil in two varieties of Piper betle from northern plains of India. J Sci Ind Res 73(2):95–99
SDAMM (Superintendent, Directorate of Agricultural Marketing, Medinipure) (1996) Paaner
Kotipoy Tathya (In Bengali). Some aspects of betel leaf. A paper presented in the Seminar-cum-
Workshop on betel leaf marketing held at the state cashew nut farm, Directorate of Agricultural
Marketing, Digha, Medinipure (W.B.), India
Sengupta K (1996) Paan Patar Sangraksan (in Bengali). Nabana Bharati 28(12):580–582
Sengupta R, Banik JK (2013) A review on betel leaf (Pan). Int J Pharm Sci Res 4(12):4519–4524
Shah B (2015) Development of a novel incense cone using unique properties of essential oil
extracted from surplus betel leaves (Piper betle L.). M. Tech thesis (under the supervision of
Prof. P. Guha), Agricultural and Food Engineering Department, Indian Institute of Technology,
Kharagpur (W.B.), India
Sharma ML, Rawat AKS, Balasubraminan VR et al (1981) Essential oil of betel leaf (Piper betle
L.) cv. Kapoori. In: Khanduja SD, Balasubrahmanyam VR (eds) Improvement in betel vine
cultivation. Proc of group discussion on Improvement of Betel Vine Cultivation. National
Botanical Research Institute, Lucknow, pp 161–164
Sharma ML, Rawat AKS, Khanna RK et al (1996) Flavour characteristics of betel leaves. Euro
Cosmet 5:22–24
Sharma S, Khan IA, Ali I et al (2009) Evaluation of the antimicrobial, antioxidant, and anti-
inflammatory activities of hydroxychavicol for its potential use as an oral care agent. Antimicrob
Agents Chemother 53(1):216–222
Shukla SV (2015). Global Scenario, market potential & business opportunities in essential oil,
fragrance & flavour. In: Flavour and Fragrance Development Center, Kannuaj, UP, India,
(ed) A report on training cum workshop on essential oil, perfumery and aromatherapy held
at New Delhi 14–16, March 2015. Available at: http://www.ffdcindia.org/pdf/global_sce-
nario_19032015.pdf. Retrieved on 04/07/2018
Shukla D, Johri J, Srivastava S et al (2018) Assessment of anticancer properties of betel vine. In:
Akhtar MS, Swamy MK (eds) Anticancer plants: properties and application, vol 1. Springer
Singapore Pte Ltd., pp 195–206
Singh KK, Balasubrahmanyam VR, Kochhar VK (1990) Effect of different packing methods, tem-
perature conditions, treatment with chemicals on the senescence and storage behaviour of betel
(Piper betle L.) leaves. J Plant Crop 18(1):23–28
Singh D, Narayanamoorthy S, Gamre S et al (2018) Hydroxychavicol, a key ingredient of Piper
betle induces bacterial cell death by DNA damage and inhibition of cell division. Free Radic
Biol Med 120:62–71
Sivasankar B (2003) Food processing and preservation. Prentice Hall of India Private Limited,
New Delhi, p 118
Slater TF (1984) Free-radical mechanisms in tissue injury. Biochem J 222:1–15
Speranza B, Corbo MR (2010) Essential oils for preserving perishable foods: possibilities and
limitations. In: Bevilacqua A, Corbo MR, Sinigaglia M (eds) Application of alternative food
preservation technologies to enhance food safety & stability. Bentham Publisher, Sharjah
(UAE), pp 35–57
196 P. Guha and S. Nandi
Sriharsha Y (2017) Utilization of spent betel leaves from essential oil industry. M. Tech thesis
(under the supervision of Prof. P. Guha), Agricultural and Food Engineering Department,
Indian Institute of Technology, Kharagpur (W.B.), India
Sugumaran M, Gandhi S, Sankarnarayanan M et al (2011) Chemical composition and antimi-
crobial activity of Vellaikodi variety of Piper betle Linn leaf oil against dental pathogens. Int
J Pharm Tech Res 3:2135–2139
Suppakul P, Sanla-ead N, Phoopuritham P (2006) Antimicrobial and antioxidant activities of betel
oil. Kasetsart J Natural Sci 40:91–100
Swapna NL, Ammani K, Prasad SHKR (2012) Antioxidant activity of Mokkathotapapada leaves
of Piper betle L. cv. Kapoori. Free Rad Antiox 2(4):68–72
Tabacchi SMR, Guerin PM (2007) Components from Sri Lankan Piper betle L. leaf oil and
their analogues showing toxicity against the housefly, Musca domestica. Flavour Fragrance
22:130–138
Thahn L, Dung NX, Luu HV et al (2002) Chemical composition of the leaf oil from Piper betle L.
cultivated in Vietnam. J Essent oil Bear plants 5(1):38–42
TNEB (The New Encyclopedia Britannica) (1974) Musk Deer. p 448
TOI (Times of India) (2014) Sterilization could make betel leaves bacteria-free: experts. Available
at: https://timesofindia.indiatimes.com/india/Sterilization-could-make-betel-leaves-bacteria-
free-Experts/articleshow/37189046.cms. Retrieved on 08/07/2018
Umar RA, Sanusi NA, Zahary MN et al (2018) Chemical composition and the potential biological
activities of Piper betle – a review. Malaysian J Appl Sci 3(1):1–8
Van de Braak SAAJ, Leijten GCJJ (1999) Essential oils and oleoresins: a survey in the Netherlands
and other major markets in the European Union. Centre for the Promotion of Imports from
Developing Countries, Rotterdam, p 116
Vasantha-srinivasan P, Senthil-nathan S, Selin-rani S et al (2016) Developmental response of
Spodoptera litura Fab. to treatments of crude volatile oil from Piper betle L. and evaluation of
toxicity to earthworm, Eudrilus eugeniae Kinb. Chemosphere 155:336–347
Vasantha-srinivasan P, Senthil-nathan S, Ponsankar A et al (2017) Comparative analysis of mos-
quito (Diptera: Culicidae: Aedes aegypti Liston) responses to the insecticide Temephos and
plant derived essential oil derived from Piper betle L. Ecotoxicol Environ Saf 139:439–446
Vijay N (2015) Development of herbal novel Lozenge with flavour of essential oil of betel leaf.
B. Tech thesis (under the supervision of Prof. P. Guha), Agricultural and Food Engineering
Department, Indian Institute of Technology, Kharagpur (W.B.), India
Vikash C, Tripati S, Verma NK et al (2012) PIPER BETEL: Phytochemistry, traditional use &
pharmacological activity – a review. Int J Pharm Res Dev 4(4):216–223
Vintilla L (2018) Basic structure, nomenclature, classification and properties of organic compound
of essential oil. In: Hashemi SMB, Khaneghah AM, de Sant’Ana AS (eds) Essential oils in food
processing: chemistry, safety and application. Wiley, New York, pp 173–177
Welke JE, Hoeltz M, Dottori HA et al (2011) Patulin accumulation in apples during storage by
Penicillium expansum and Penicillium griseofulvum strains. Braz J Microbiol 42:172–180
Wendy VWY, Ghali NA, Rukayadi Y et al (2014) Application of betel leaves (Piper betle L.)
extract for preservation of homemade chili bo. Int Food Res J 21(6):2399–2403
Wikipedia (2018a) Deer Musk. Available at: https://en.wikipedia.org/wiki/Deer_musk. Retrieved
on 01/07/2018
Wikipedia (2018b) Kannuaj perfume. Available at: https://en.wikipedia.org/wiki/Kannauj_
Perfume. Retrieved on 26/08/2018
Wikipedia (2018c) Geosmin. Available at: https://en.wikipedia.org/wiki/Geosmin. Retrieved on
26/08/2018
Zahin M, Khan MS, Qais FA et al (2018) Antioxidant properties and anti-mutagenic potential of
Piper cubeba fruit extract and molecular docking of certain bioactive compounds. Drug Chem
Toxicol 41(3):358–367
Zielińska S, Matkowski A (2014) Phytochemistry and bioactivity of aromatic and medicinal plants
from the genus Agastache (Lamiaceae). Phytochem Reviews 13(2):391–416
Chapter 6
Artemisia annua and Artemisia afra
Essential Oils and Their Therapeutic
Potential
Abbreviations
6.1 Introduction
For millennia, Artemisia annua L. was used by the Chinese to treat fever, which was
often thought to be malaria (Hsu 2006; Tu 2011). The sesquiterpene lactone,
artemisinin, is considered the main antimalarial phytochemical in A. annua
(Fig. 6.1). However, many constituents of the plant’s essential oils (EOs) including
1,8-cineole (eucalyptol), limonene, myrcene, α- and β-pinenes, and nerolidol also
are known to be antimalarial as isolated chemicals, albeit with much less effective
inhibitory concentrations (IC50 values) than artemisinin (see reviews by Weathers
et al. 2014, 2017). There is evidence, however, suggesting that at least in some
cases, the EO fraction per se is more potent than its individual constituents
(Radulović et al. 2013). A. afra has also been used by native Africans to treat malaria
(Liu et al. 2009; Watt and Breyer-Brandwijk 1962). While considerable information
is known about the breadth of the medicinal properties of A. annua to treat not only
malaria as well as other diseases, A. afra has only recently attracted more attention
for its healing properties (Patil et al. 2011). Although their composition is somewhat
different, constituents of the EOs of each species seem to play a role in the therapeutic
efficacy of both plant species. Here, we summarize what is currently known about
the EO fraction of these two important medicinal plant species and how the
phytochemicals therein may affect therapeutic outcomes.
Fig. 6.1 Representative leaves of the same developmental age from three cultivars of Artemisia
annua and one A. afra along with structure of artemisinin. Clockwise from top left: A. annua SAM,
#15, GLS, A. afra
One of the major difficulties in studying EOs from A. annua is the inconsistency of
the oil contents. From cultivar to cultivar, the major constituents of the oil can
change dramatically. Even within cultivars, factors including geographic location,
growth conditions, method of propagation, and stage of development at harvest can
change the contents of the oil. For example, our lab performed a phytochemical
analysis of A. annua essential oil sourced from the United States or China. The
resulting peak areas from the GC-MS analysis can be seen in Table 6.1 (Desrosiers
and Weathers 2017). As expected, both oils contained mostly monoterpenes;
however, the oil sourced in the United States contained almost 31% thujone, a
compound not detectable in the Chinese-sourced oil. Although thujone is
characteristically found in A. afra and A. absinthium, A. annua is consistently
reported to be thujone-free, which aids in its validation as a generally recognized as
safe (GRAS) plant (Duke 2001). The presence of thujone, therefore, suggests the
US-sourced EO was adulterated with oil from other Artemisia species. The
US-sourced oil contained about 30% camphor, while the Chinese oil only contained
about 14% of the same. Such dramatic differences in EO composition even within
the same plant species (see below) make studying the oil and establishing reliable
scientific conclusions difficult. Comparisons between labs are nearly impossible,
and this raises questions about therapeutic uses of the oil if a consistent product
cannot be produced on a large scale. This also highlights the dangers of using
unregulated EO products to treat medical conditions. An EO of undefined chemical
makeup cannot be trusted to consistently treat any medical condition and may even
have undesirable health effects.
6 Artemisia annua and Artemisia afra Essential Oils and Their Therapeutic… 199
Table 6.1 Relative abundance of some phytochemicals identified by GC-MS in A. annua essential
oil from US and Chinese sources (Desrosiers and Weathers 2017)
US EO source Chinese EO source
Phytochemical % of total peak area % of total peak area
1,8-Cineole (eucalyptol) 16.5 27.4
α-Pinene 0.3 17.5
β-Pinene 2.5 4.7
Borneol 3.7 1.8
Camphene 13.2 5.5
Camphor 30.3 14.1
Carene Nd 2.2
Caryophyllene 1.0 6.2
Caryophyllene oxide 0.9 Nd
Copaene Nd 1.1
Humulene Nd 5.5
Limonene Nd 5.2
Myrcene 0.1 Nd
Phellandrene Nd 8.5
Santolina triene 0.1 0.2
Stigmasterol 0.1 Nd
Terpineol 0.2 Nd
Thujone 30.9 Nd
EO essential oil, Nd not detected
Why A. afra? This species is native to southern Africa and has been used as a tea
infusion by indigenous peoples to treat fevers, especially associated with malaria
(Watt and Breyer-Brandwijk 1962). Many other medicinal properties are ascribed to
the plant; these are well summarized in two relatively recent reviews (Liu et al.
2009; Patil et al. 2011). Recently, tea infusions of A. afra and A. annua performed
faster than praziquantel in treating schistosomiasis in a human clinical
trial (Munyangi et al. 2018). The oil also shows antimicrobial activity against some
bacteria and yeast species. Unfortunately, most of the studies express antibiotic
activity through a series of oil dilutions or by zones of inhibition; only a few studies
provide IC50 values, statistical analysis of minimum inhibitory concentration (MIC),
or minimum inhibitory percentage (MIP). In a recent malaria clinical trial, a tea
infusion of A. afra performed similarly to A. annua, and both were better than
artemisinin combination therapy (ACT) in their therapeutic efficacy and reduction
of gametocyte carriage (Munyangi et al. 2019). Interestingly, A. afra used in that
study only had trace amounts of artemisinin.
Over 130 volatile chemicals constituting A. afra EO were identified and docu-
mented in a review by Liu et al. (Liu et al. 2009). The most common volatiles in A.
afra include artemisyl acetate, 1,8-cineole, α- and β-thujone, artemisia ketone,
α-copaene, camphor, santolina alcohol, borneol, and camphene (Liu et al. 2009). As
observed for A. annua, these EO phytochemicals change in quantity and quality
with plant part, among cultivars, throughout development, with cultivation, and
with processing method. For example, surveying some of the volatile constituents
of dried leaf samples of three cultivars analyzed in our lab, camphor can vary by
threefold and thujone can be present or absent (Table 6.2). Drying methods also
substantially alter volatile components from 0.18% to 1.88% from fresh to dried
material (Asekun et al. 2007). Considering that fresh material has about ten times
the water of dried material, these percent values are not particularly different.
However, drying did change the relative composition of individual phytochemicals
within the oil. As an example, artemisia ketone was present in fresh material but
absent in air- and sun-dried material (Asekun et al. 2007). When comparing the
effects of microwave-drying against air-, sun-, and oven-dried material, the
monoterpenes, 1,8-cineole and β-thujone (but not α-thujone) decreased, while
several other compounds increased, particularly trans-caryophyllene (Ashafa and
Pitso 2014).
Artemisinin from A. annua has been widely studied for its antimalarial activity, and
several derivatives have been developed as ACTs and are in use to combat malaria
worldwide. However, the EO produced by Artemisia species also has wide-ranging
antimicrobial properties. Many have pondered the evolutionary benefits of producing
202 M. R. Desrosiers et al.
EO, as well as artemisinin, to the A. annua plant. Some have speculated these
compounds acted as antimicrobials and insecticides to deter herbivorous insects and
pathogenic microbes. There is indeed evidence for these hypotheses, as several
studies have shown A. annua EO to have activity against common bacterial and
fungal strains, with examples shown in Table 6.3.
While the EO of A. annua has shown some promise in vitro, there are few studies
in vivo and there are still several questions surrounding the use of EOs as a
therapeutic. For example, the proper way to deliver EOs is not clear. For certain
external infections, topical application may suffice, but in vivo studies and clinical
trials would have to be performed to establish efficacy, dosage, and safety.
Furthermore, it is unclear whether or not it is economically feasible to produce EOs
on a large enough scale to be used as a therapeutic. Large-scale production of EOs
requires steam distillation of large amounts of plant material to produce a small
amount of oil, and for this reason, it may simply be too expensive to rely on EOs as
antimicrobials.
Although any pure EO can be toxic, consumption of A. annua EOs as part of dried
leaf material or a tea infusion is nontoxic; the plant is GRAS, and tea infusions have
been consumed for millennia. A. afra may contain thujone in its EO, which is
considered toxic and, therefore, regulated. Thujones act on the central nervous
system as antagonists of γ-aminobutyric acid-A (GABAA) (Höld et al. 2000) and
5-hydroxytryptamine (5-HT3, or serotonin) (Deiml et al. 2004), but the toxicity of
A. afra is debatable. Interestingly, the EU restricts thujone to 0.5 mg kg−1 in food
prepared with Artemisia species, but in those made with sage, which also contains
the monoterpene, the limit is 25 mg kg−1. There are also similar types of restrictions
for foods and beverages in the USA and Canada, again with exceptions for use of
the herb, sage. In rats, the per os LD50 is 500 mg kg−1, and thus, naturally occurring
levels of thujone in A. afra are too low from orally consumed plant material either
as powdered dried leaves or as tea infusions to be considered toxic (Table 6.2).
Additionally, only a small fraction of the thujone present in plant material is
extracted into water compared to an ethanolic formulation (Tegtmeier and
Harnischfeger 1994).
6.3.3 P
rocess Caveats: Losses with Drying, Storage,
Powdering, Tableting
Fresh A. annua plant material has a different EO profile versus dried, processed
leaves, mainly due to the volatile nature of the monoterpene components. After
fresh leaves were dried, sieved, and powdered, camphor and 1,8-cineole content
decreased. Only camphor remained detectable after powdered leaves were processed
into tablets via mechanical compression (Table 6.4; adapted from Weathers and
Towler 2014). Powdering dried leaf material with a blade mill generates only slight
heat when performed in short pulses. However, operation of the machinery required
to form the tablets can cause an increase in processing temperature, which can
Table 6.4 Selected compounds in A. annua and effect of drying, granulation, and tablet formation
Fresh leavesa Dried and sieved Powdered Tablets
Compound mg g−1 DW−1 mg g−1 DW−1 mg g−1 DW−1 mg g−1 DW−1
1,8-cineole 0.30 0.03 0.03 Nd
Camphor 3.57 2.10 1.67 0.19
Artemisinin 11.38 15.90 17.31 17.18
Total flavonoids # 1.55 2.78 5.05 10.97
a
DW calculated using DW/FW ratio of 0.25. Methylene chloride extract assayed by GC-MS,
except total flavonoids assayed by colorimetric aluminum chloride assay. Each condition had
n ≥ 6; nd = not detected; #, expressed as quercetin equivalents; all others quantified with authentic
standards and identified using NIST library
204 M. R. Desrosiers et al.
6.4 Bioavailability
Beyond its direct bioactivity against microbes, the EO of A. annua has effects on the
bioavailability of the main drug of interest in A. annua, artemisinin, at least indirectly
increasing the therapeutic efficacy of dried leaf treatment. The oral bioavailability
of any drug is influenced by several factors as illustrated in Fig. 6.2. An orally
delivered drug is first subject to low pH and digestive enzymes in the stomach. It is
then subject to more enzymatic activity, bile, and more neutral pH in the small
intestine. Most absorption occurs in the intestine where solubility of the drug has
significant effects on how well the drug is absorbed. The drug also is subjected to
limited first-pass metabolism as it is transported across the intestine and then travels
Fig. 6.2 Schematic of the steps orally delivered drugs take to reach systemic circulation
6 Artemisia annua and Artemisia afra Essential Oils and Their Therapeutic… 205
through the hepatic portal vein to the liver where it is then subjected to significant
metabolism before reaching systemic circulation. A. annua EO may have effects at
each stage in this process, and those effects are discussed here. For a more
comprehensive review of oral drug bioavailability, please see Song et al. (2004).
A. annua EOs are largely found in the glandular trichomes where they co-localize
with artemisinin, allowing it to stay in solution. Traditionally, artemisinin is known
to have low bioavailability due largely in part to its low aqueous solubility. In its
pure form, the drug does not readily solubilize in aqueous environments like the
stomach or intestine, leading to very low absorption into the blood. As a result, pure
artemisinin treatment is only marginally effective against malaria because most of
the drug does not reach systemic circulation where it is required to act on the
Plasmodium parasites. Consequently, the derivatives of artemisinin currently used
in ACTs were developed to have increased aqueous solubility. Interestingly, when
artemisinin is delivered as plant material, artemisinin solubility is about fourfold
higher in simulated intestinal fluid (Desrosiers and Weathers 2016). EO from the
plant is largely responsible for this effect. When A. annua EO was combined with
pure artemisinin and subjected to simulated digestion experiments, the solubility in
simulated intestinal fluid increased 2.5-fold (Desrosiers and Weathers 2016). This
effect on solubility partially explains why artemisinin delivered as dried A. annua
leaves was >40× more bioavailable than pure drug in mice (Weathers et al. 2011).
Other antimalarial phytochemicals in A. annua also have low aqueous solubility; for
example, the flavonoids, which are reported to synergistically enhance antimalarial
efficacy of artemisinin (Liu et al. 1992; Suberu et al. 2013). Bioavailability of these
phytochemicals may also be increased. Thus, by increasing solubility of artemisinin
and other phytochemicals, the EO in the plant also increases the bioavailability of
these compounds, further enhancing the antiplasmodial efficacy of artemisinin
delivered as powdered dried plant leaves.
artemisinin were digested together with pure artemisinin using the simulated
system, the permeability of artemisinin was unchanged compared to pure artemisinin
controls (Desrosiers and Weathers 2017). These data suggested that A. annua EO
decreased permeability on its own and that this decrease was nullified by the bulk of
the plant matrix.
The full story of how A. annua EOs affect artemisinin bioavailability is not com-
plete without understanding how EOs affect metabolism in the liver, where artemis-
inin is known to undergo significant first-pass metabolism. In this scenario,
artemisinin is metabolized into four metabolites: deoxyartemisinin, deoxydihydro-
artemisinin, 9,10-dihydrodeoxyartemisinin, and crystal 7 (Lee and Hufford 1990).
None has any antimalarial activity mainly due to the loss of the endoperoxide bridge
responsible for the drug’s potent activity against Plasmodium. In addition to solubil-
ity concerns, the high first-pass metabolism of artemisinin is another reason for the
development of the semi-synthetic artemisinin derivatives currently used in ACTs.
These semi-synthetic derivatives are metabolized in the liver into dihydroartemis-
inin, which retains the endoperoxide bridge and potent antiplasmodial activity (Lee
and Hufford 1990; Navaratnam et al. 2000).
Several compounds found in the EO of A. annua may, however, modulate first-
pass liver metabolism of artemisinin in a way that allows more of the drug to reach
systemic circulation. For example, camphor, one of the components found in both
A. annua and A. afra EOs, inhibits CYP2B6, the main enzyme responsible for
artemisinin metabolism (Seo et al. 2008; Svensson and Ashton 1999). By inhibiting
CYP2B6, camphor present in the oil would allow more artemisinin to bypass
metabolism in the liver and reach systemic circulation. Borneol, limonene, and
cineol also inhibited CYP2B6. More studies are needed to determine if other
components in A. annua EO inhibit CYP2B6 as well as CYP3A4, which is also
partially involved in artemisinin metabolism.
Table 6.5 Repellant and insecticidal activity of A. annua essential oil and essential oil components
on various insect species
A. annua component Species Activity Source
A. annua alcoholic Cydia pomonella Repellant Durden et al.
extract (2011)
Artemisinin
1,8-Cineole
A. annua EO Calliphora vomitoria Insecticidal Bedini et al.
(2017)
A. annua EO Tribolium castaneum Repellant and Tripathi et al.
insecticidal (2000)
Callosobruchus Insecticidal
maculatus
however, to our knowledge, no reliable studies have been conducted to date to vali-
date this claim. Many of the EOs found in A. annua are also in A. afra and would be
expected to provide similar responses.
6.6 Conclusions
While EOs per se are not recommended as direct therapeutic agents, their inclusion
in an herbal or other medicinal preparation may have profound effects on therapeutic
outcomes. Evidence shows that EOs from A. annua and A. afra may not only have
direct therapeutic effects against various ailments including infectious diseases but
also enhance the bioavailability of more potent phytochemical drugs, e.g.,
artemisinin. The mechanism of action of EO effects include improved solubility of
otherwise poorly soluble compounds and possible inhibition of liver metabolism by
cytochrome P450s. Overall, this information enhances our understanding of the role
of EOs in therapeutic medicinal applications.
References
Asekun OT, Grierson DS, Afolayan AJ (2007) Variations in the quality and yield of the essential oil
from Artemisia afra using different drying methods. J Essent Oil Bear Pl 10(1):5–9
Ashafa AOT, Pitso TR (2014) Effects of microwave drying on the yield and chemical composition
of essential oils of Artemisia afra Jacq. Ex willd from the Eastern Free State South Africa.
J Essent Oil Bear Pl 17(6):1087–1093
208 M. R. Desrosiers et al.
Augustijns P, D’Hulst A, Van Daele J, Kinget R (1996) Transport of artemisinin and sodium arte-
sunate in Caco-2 intestinal epithelial cells. J Pharm Sci 85(6):577–579
Bagchi GD, Haider F, Dwivedi PD, Singh A, Naqvi AA (2003) Essential oil constituents of
Artemisia annua during different growth periods at monsoon conditions of subtropical north
Indian plains. J Essent Oil Res 15(4):248–250
Bedini S, Flamini G, Cosci F, Ascrizzi R, Echeverria MC, Guidi L, Landi M, Lucchi A, Conti B
(2017) Artemisia spp. essential oils against the disease-carrying blowfly Calliphora vomitoria.
Parasit Vectors 10(1):80
Deiml T, Haseneder R, Zieglgänsberger W, Rammes G, Eisensamer B, Rupprecht R, Hapfelmeier
G (2004) α-Thujone reduces 5-HT 3 receptor activity by an effect on the agonist-induced
desensitization. Neuropharmacology 46(2):192–201
Desrosiers MR, Weathers PJ (2016) Effect of leaf digestion and artemisinin solubility for use in oral
consumption of dried Artemisia annua leaves to treat malaria. J Ethnopharmacol 190:313–318
Desrosiers MR, Weathers PJ (2017) Artemisinin permeability via Caco-2 cells increases after
simulated digestion of Artemisia annua leaves. J Ethnopharmacol
Duke JA (2001) Handbook of phytochemical constituents of GRAS herbs and other economic
plants. CRC Press LLC, Boca Raton, FL
Durden K, Sellars S, Cowell B, Brown JJ, Pszczolkowski MA (2011) Artemisia annua extracts,
artemisinin and 1, 8-cineole, prevent fruit infestation by a major, cosmopolitan pest of apples.
Pharm Biol 49(6):563–568
Hsu E (2006) The history of qing hao in the Chinese materia medica. Trans R Soc Trop Med Hyg
100(6):505–508
Höld KM, Sirisoma NS, Ikeda T, Narahashi T, Casida JE (2000) α-Thujone (the active component
of absinthe): γ-aminobutyric acid type A receptor modulation and metabolic detoxification.
Proc Natl Acad Sci 97(8):3826–3831
Juteau F, Masotti V, Bessière JM, Dherbomez M, Viano J (2002) Antibacterial and antioxidant
activities of Artemisia annua essential oil. Fitoterapia 73(6):532–535
Lee I-S, Hufford CD (1990) Metabolism of antimalarial sesquiterpene lactones. Pharmacol Ther
48(3):345–355
Li Y, Hu H-b, Zheng X-d, Zhu J-h, Liu L-p (2011) Composition and antimicrobial activity of
essential oil from the aerial part of Artemisia annua. J Med Plant Res 5(16):3629–3633
Liu KC-SC, Yang S-L, Roberts MF, Elford BC, Phillipson JD (1992) Antimalarial activity of
Artemisia annua flavonoids from whole plants and cell cultures. Plant Cell Rep 11(12):637–640
Liu NQ, Van der Kooy F, Verpoorte R (2009) Artemisia afra: a potential flagship for African
medicinal plants? S Afr J Bot 75(2):185–195
Massiha A, Majid Khoshkholgh-Pahlavian M, Issazadeh K, Bidarigh S, Zarrabi S (2013)
Antibacterial activity of essential oils and plant extracts of Artemisia (Artemisia annua L.)
in vitro. Zahedan J Res Med Sci 15(6):14–18
Munyangi J, Cornet-Vernet L, Idumbo M, Lu C, Lutgen P, Perronne C, Ngombe N, Bianga
J, Mupenda B, Lalukala P, Mergeai G, Mumba D, Towler M, Weathers P (2018) Effect of
Artemisia annua and Artemisia afra tea infusions on schistosomiasis in a large clinical trial.
Phytomedicine 51:233–240
Munyangi J, Cornet-Vernet L, Idumbo M, Lu C, Lutgen P, Perronne C, Ngombe N, Bianga J,
Mupenda B, Lalukala P, Mergeai G, Mumba D, Towler M, Weathers P (2019) Artemisia annua
and Artemisia afra tea infusions vs. artesunate-amodiaquine (ASAQ) in treating Plasmodium
falciparum malaria in a large scale, double blind, randomized clinical trial. Phytomedicine
57:49–56
Navaratnam V, Mansor SM, Sit N-W, Grace J, Li Q, Olliaro P (2000) Pharmacokinetics of artemis-
inin-type compounds. Clin Pharmacokinet 39(4):255–270
Patil GV, Dass SK, Chandra R (2011) Artemisia afra and modern diseases. J Pharmacogenomics
Pharmacoproteomics 2(3)
Radulović NS, Randjelović PJ, Stojanović NM, Blagojević PD, Stojanović-Radić ZZ, Ilić IR,
Djordjević VB (2013) Toxic essential oils. Part II: chemical, toxicological, pharmacological
and microbiological profiles of Artemisia annua L. volatiles. Food Chem Toxicol 58:37–49
6 Artemisia annua and Artemisia afra Essential Oils and Their Therapeutic… 209
Rana VS, Abirami K, Blázquez MA, Maiti S (2013) Essential oil composition of Artemisia annua
L. at different growth stages. J Spices Aromat Crops 22(2):181–187
Seo K-A, Kim H, Ku H-Y, Ahn H-J, Park S-J, Bae SK, Shin J-G, Liu K-H (2008) The monoterpe-
noids citral and geraniol are moderate inhibitors of CYP2B6 hydroxylase activity. Chem Biol
Interact 174(3):141–146
Song N-N, Zhang S-Y, Liu C-X (2004) Overview of factors affecting oral drug absorption. Asian
J Drug Metab Pharmacokinet 4(3):167–176
Suberu JO, Gorka AP, Jacobs L, Roepe PD, Sullivan N, Barker GC, Lapkin AA (2013) Anti-
plasmodial polyvalent interactions in Artemisia annua L. aqueous extract – possible synergistic
and resistance mechanisms. PLoS One 8(11):e80790
Svensson USH, Ashton M (1999) Identification of the human cytochrome P450 enzymes involved
in the in vitro metabolism of artemisinin. Br J Clin Pharmacol 48(4):528–535
Tegtmeier M, Harnischfeger G (1994) Methods for the reduction of thujone content in pharma-
ceutical preparations of Artemisia, Salvia and Thuja. Eur J Pharm Biopharm 40(5):337–340
Towler MJ, Weathers PJ (2015) Variations in key artemisinic and other metabolites throughout plant
development in Artemisia annua L. for potential therapeutic use. Ind Crop Prod 67:185–191
Tripathi AK, Prajapati V, Aggarwal KK, Khanuja SPS, Kumar S (2000) Repellency and toxicity
of oil from Artemisia annua to certain stored-product beetles. J Econ Entomol 93(1):43–47
Tu Y (2011) The discovery of artemisinin (qinghaosu) and gifts from Chinese medicine. Nat Med
17(10):1217–1220
Tzenkova R, Kamenarska Z, Draganov A, Atanassov A (2010) Composition of Artemisia annua
essential oil obtained from species growing wild in Bulgaria. Biotechnol Biotechnol Equip
24(2):1833–1835
Verdian-Rizi MR, Sadat-Ebrahimi E, Hadjiakhoondi A, Fazeli MR, Pirali Hamedani M (2008)
Chemical composition and antimicrobial activity of Artemisia annua L. essential oil from Iran.
J Med Plant 1(25):58–62
Viuda-Martos M, El Gendy AE-NGS, Sendra E, Fernandez-Lopez J, Abd El Razik KA, Omer EA,
Pérez-Alvarez JA (2010) Chemical composition and antioxidant and anti-Listeria activities
of essential oils obtained from some Egyptian plants. J Agric Food Chem 58(16):9063–9070
Watt JM, Breyer-Brandwijk MG (1962) The medicinal and poisonous plants of Southern Africa
and Eastern Africa: being an account of their medicinal and other uses, chemical composition,
pharmacological effects and toxicology in man and animal. E.S. Livingstone, Edinburgh
Weathers PJ, Arsenault PR, Covello PS, McMickle A, Teoh KH, Reed DW (2011) Artemisinin
production in Artemisia annua: studies in planta and results of a novel delivery method for
treating malaria and other neglected diseases. Phytochem Rev 10(2):173–183
Weathers PJ, Cambra HM, Desrosiers MR, Rassias D, Towler MJ (2017) Artemisinin the nobel
molecule: from plant to patient. In: Studies in natural products chemistry. Elsevier, Amsterdam,
pp 193–229
Weathers PJ, Towler M, Hassanali A, Lutgen P, Engeu PO (2014) Dried-leaf Artemisia annua:
a practical malaria therapeutic for developing countries? World Journal of Pharmacology
3(4):39–55
Weathers PJ, Towler MJ (2014) Changes in key constituents of clonally propagated Artemisia
annua L. during preparation of compressed leaf tablets for possible therapeutic use. Ind Crop
Prod 62:173–178
Yang Z-n, Zhu S-q, Yu Z-w (2012) Comparison of terpene components from flowers of Artemisia
annua. Bangladesh J Pharmacol 7(2):114–119
Chapter 7
Outstanding Efficacy of Essential Oils
Against Oral Pathogens
7.1 Introduction
The oral cavity is a favorable environment for various microorganisms where they
exist in multispecies communities (Borges et al. 2015). Bacterial community, which
is attached to organic or inorganic surfaces, is called biofilm. Bacteria are embedded
in an extracellular polymeric matrix which they produce themselves (Mashima and
Nakazawa 2014).
An exquisite example of naturally formed biofilm is a dental plaque (Mancl et al.
2013). It is considered the main cause of tooth decay (caries) that can lead to root
canal infections; and also biofilm can affect the tooth supporting tissues and cause
J. Marinković
“Vinča” Institute of Nuclear Sciences, Mike Petrovića Alasa 12,
University of Belgrade, Belgrade, Serbia
e-mail: [email protected]
T. Marković
Institute for Medicinal Plant Research “Dr. Josif Pančić”, Tadeuša Koščuška 1,
Belgrade, Serbia
e-mail: [email protected]
B. Miličić
Department for Medical Statistics and Informatics, School of Dental Medicine,
Dr. Subotića 1, University of Belgrade, Belgrade, Serbia
e-mail: [email protected]
M. Soković (*) · A. Ćirić
Institute for Biological Research “Siniša Stanković”, Bulevar Despota Stefana 142,
University of Belgrade, Belgrade, Serbia
e-mail: [email protected]; [email protected]
D. Marković
Department of Pediatric and Preventive Dentistry, School of Dental Medicine,
Dr. Subotića 11, University of Belgrade, Belgrade, Serbia
e-mail: [email protected]
Fig. 7.2 S. sanguinis and S. salivarius: members of biofilm community. Both species have an
important role in first-stage development of disease of tooth-supporting tissues. An example of
gingivitis and gingival hypertrophy
Fig. 7.3 Streptococcus salivarius (first row) and Streptococcus sanguinis (second row)
7.2 M
ultiple Roles of Streptococcus sanguinis
and Streptococcus salivarius
As specific behavior of these oral pathogens and their interactions with other bacte-
ria make them very important in dental biofilm formation, their multiple roles
deserve to be described in more detail.
According to Caufield et al. (2000) and Kreth et al. (2005), there is antagonism
between S. sanguinis and S. mutans. Bearing in mind that different Streptococcus
species compete for similar surviving and growing conditions (Zhu and Kreth
2010), the role of S. sanguinis in caries development might be defined as antagonis-
tic toward S. mutans; its activity is mainly based on inhibition of S. mutans which is
achieved by the release of hydrogen peroxide it produces (Carlsson et al. 1983;
Kreth et al. 2005), in addition to the fact that it also secrets antimicrobial agent
named bacteriocin, sanguicin (Fujimura and Nakamura 1979). Similarly, S. mutans
developed anti-S. sanguinis strategy which includes production of antimicrobial
peptides mutacin I and IV, both capable of inhibiting S. sanguinis (Kreth et al.
2005). An obvious antagonism between these two pathogens, particularly pro-
nounced under the aerobic conditions, is controlled by the fact which one will be
first inoculated, and it is crucial for the entire caries development procedure (Kreth
et al. 2008). The synthesis of hydrogen peroxide by S. sanguinis lies in its capability
to metabolize carbohydrates and produce lactate and pyruvate via glycolytic path-
ways. The enzyme pyruvate oxidase catalyzes conversion of pyruvate, inorganic
phosphate, and oxygen to hydrogen peroxide, carbon dioxide, and acetyl phosphate;
this provides S. sanguinis an effective H2O2 mechanism which enables it to resist
other bacteria, at the same time being resistant to itself (Carlsson and Edlund 1987;
Zheng et al. 2011; Zhu and Kreth 2012).
Antagonism of S. sanguinis is not specific only against cariogenic bacteria S.
mutans but is also observed toward the periodontopathogens which cause the most
common oral disease, periodontitis. This might be illustrated by the prevalence of S.
sanguinis in healthy subjects in comparison with patients with periodontitis (Stingu
et al. 2008). Periodontopatoghens, such as Porphyromonas gingivalis, Prevotella
intermedia, and Aggregatibacter actinomycetemcomitans, showed to be sensitive to
the hydrogen peroxide produced by S. sanguinis; the achieved inhibition seems to
be time dependent and was only observed when S. sanguinis were spotted 24 h
before spotting the other pathogens. Also, the inhibition was favored under the aero-
bic then anaerobic condition (Herrero et al. 2016).
serious and potentially fatal infection obtained through interaction of the two oral
pathogens, S. sanguinis and S. salivarius, with matrix molecules and platelets at
sites of endocardial cell damage (Di Filippo et al. 2006; Kao et al. 2013; Wilson
et al. 2008).
The oral cavity is the entrance gate for these two bacteria. Bacteremia can be
provoked by some every day routine activities, such as tooth brushing, flossing,
usage of wooden toothpicks and chewing a food (Wilson et al. 2008), or it can
appear following a common dental treatment such as a tooth cleaning (Di Filippo
et al. 2006; Laura et al. 2014) or tooth extraction (Wilson et al. 2008). Also, impor-
tant source and provoking cause of bacteremia may be a dental infection, originat-
ing weather from the tooth or its supporting tissues, and it is considered as a dental
focus (Di Filippo et al. 2006; Wilson et al. 2008; Wisniewska-Spychala et al. 2012).
S. sanguinis and S. salivarius related to subacute endocarditis were isolated from
the following dental foci: teeth with necrotic pulp or with its necrotic decay, resected
teeth with poorly filled root canals due to anatomical or other difficulties, and teeth
without evident periapical lesions and with seemingly well-filled root canal, such as
endo-perio syndrome (Wisniewska-Spychala et al. 2012). All these lead to the fact
that endocarditis is certainly related to untreated or badly treated S. sanguinis- and
S. salivarius-rich infected root canals.
If we consider that only sterile root canal is the healthy one, then none of the
beneficial bacteria nor their interaction with more or less pathogenic bacteria may
find its place or role in the root canal system. With regard to this, the dental root
canal resembles the blood stream; only the sterile ones may guarantee survival.
Streptococcus spp., including S. sanguinis and S. salivarius, are well known by its
presence in infected root canal (Fouad et al. 2003). They use to be found in primary
infected dental root canals where infection is caused by microorganisms that ini-
tially invade and colonize the necrotic pulp tissue (Gomes et al. 2004; Lew et al.
2015; Siqueira and Rôças 2009) or they use to be isolated from the root canals of
already treated teeth (Gomes et al. 2004; Łysakowska et al. 2016, Rocas and Siqueira
2012). Further, they can be part of a canal flora in cases of canal infection compli-
cated with symptomatic or asymptomatic, acute or chronic periapical periodontitis
(Jacinito et al. 2003; Provenzano et al. 2015; Rolph et al. 2001; Rocas and Siqueira
2012; Siqueira et al. 2007; Tatikonda et al. 2017).
S. sanguinis proved to be capable of infecting the root canal system within a
week time period (Shovelton 1959). The SEM microscopy analysis confirmed that,
while in combination with Fusobacterium nucleatum or with Actinomyces oris, it
penetrates into the dental tubules (Stauffacher et al. 2017), which usually occurs
between the 20th and 28th day. Its penetration length ranges from 150 to 792 μm
7 Outstanding Efficacy of Essential Oils Against Oral Pathogens 217
(Berkiten et al. 2000; Perez et al. 1993). Bearing in mind that bacteria localized
deep inside the dentin tubules could not be removed by the endodontic procedure,
as they are not easily accessed by the instruments or irrigation solutions (Matsuo
et al. 2003), it’s easy to understand why the members of Streptococcus spp. repre-
sent crucial portion of dental flora associated to root infections (Gajan et al. 2009;
Gomes et al. 2004; Lysakowska et al. 2016; Siqueira and Rocas 2008).
Resistance of S. sanguinis and S. salivarius to endodontic treatment is related to
their capabilities to survive chemo-mechanical root canal preparation (Lew et al.
2015; Sakamoto et al. 2007), while the intra-canal medication with Ca(OH)2 paste
following the chemo-mechanical procedure represents the last stage of defense
against these two bacteria prior to dental canal filling (Siqueira and Rocas 2008).
Apart from already known resistance of Enterococcus faecalis (Abbaszadegan et al.
2016), S. sanguinis and S. salivarius also proved to be alkaline-tolerant and capable
to survive Ca(OH)2 dressing (Lew et al. 2015; Sakamoto et al. 2007), which leads to
a conclusion that standard endodontic procedure lacks in efficiency with regard to
the mentioned canal bacteria. Although not every patient with infected root canal
necessarily suffer bacteremia, severity of described systemic diseases makes bacte-
rial eradication very important.
Bearing in mind emerging antimicrobial resistance to antimicrobial agents,
accompanied with a fact that EOs are proved to be good alternative toward standard
antimicrobial therapy with no resistance issues, we are searching for EO which will
possess high potential toward both S. sanguinis and S. salivarius and be capable of
disabling their ability of forming biofilm and consequently avoid most common oral
diseases.
7.3 P
rocedure for Screening the Literature on High-Efficacy
Essential Oils Against Two Oral Pathogens
Original papers have been selected from the available index bases, the Web of
Science, Scopus, and Medline, in order to select results from available scientific
literature and study them together with results from our previous investigation
(Nikolić 2015; Nikolić et al. 2014, 2016). The keywords used in the searching pro-
cedure were essential oil and Streptococcus sanguinis/Streptococcus sanguis;
essential oil and S. sanguinis/sanguis; essent* oil* and S. sanguinis/sanguis; essen-
tial oil and Streptococcus salivarius; essential oil and S. salivarius; and essent* oil*
and S. salivarius. There was no time span limitation. All manuscripts presenting
antimicrobial effect of EOs on S. sanguinis and/or S. salivarius, fulfilling the fol-
lowing pre-set criteria were included:
• With chemical composition of tested EOs
• With clinical isolates of S. sanguinis and/or its ATCC 10556 referent strain
• With clinical isolates of S. salivarius subsp. salivarius and/or its ATCC 9222
referent strain
218 J. Marinković et al.
• With the CLSI recommendation standard for Streptococcus spp. Viridans group
(CLSI 2013) (which includes ATCC referent strains of S. sanguinis and S.
salivarius)
• With Streptococcus sanguinis ATCC® 10556™ manufacturer strain product
sheet recommendation
• With S. salivarius subsp. salivarius ATCC® 9222™ manufacturer strain product
sheet recommendation
• With the use of microdilution method (Fig. 7.4) for MIC values determination, in
which the MIC values were ≤250 μL of EO mL−1 of growing medium
According to the achieved MIC values, selected EOs were divided into two
groups: the EOs with strong antimicrobial activity (MIC ranged from 0.0001 to
10 μL mL−1) and the EOs with good antimicrobial activity (MIC values ranged from
10 to 250 μL mL−1).
7.4 A
ntimicrobial Activity of Essential Oils and Their
Constituents Toward Streptococcus salivarius
and Streptococcus sanguinis, Clinical and ATCC Strains
7.4.1 G
eneral Variation in Response of Streptococcus
sanguinis and Streptococcus salivarius to Selected
Essential Oils
Out of 21 selected EOs (Table 7.1), 12 EOs showed identical MIC values against
both pathogens: ten EOs (Thymus serpyllum, T. algeriensis, Leptospermum peterso-
nii, Eucalyptus citriodora, Melaleuca quinquenervia, Rosa centifolia, Syzygium
aromaticum, Hyssopus officinalis, Rosmarinus officinalis, and Pelargonium graveo-
lens) against the clinical isolates and the remaining two EOs (Satureja khuzestanica
and Carum copticum) against the referent ATCC strains.
Table 7.1 Comparative presentation of the MIC values of 21 EOs with satisfactory efficacy
(MIC£250 μg mL1) on two oral Streptococcus species (clinical isolates and corresponding ATCC
strains)
Essential oils MIC values Reference
(EOs) S. salivarius S. sanguinis
Clinical Thymus serpyllum 3 μg mL−1 3 μg mL−1 Nikolic et al. (2014)
isolates Thymus algeriensis 40 μg mL−1 40 μg mL−1 Nikolic et al. (2014)
Satureja montana 60 μg mL−1 30 μg mL−1 Nikolic (2015)
Leptospermum 60 μg mL−1 60 μg mL−1 Nikolic (2015)
petersonii
Thymus vulgare 80 μg mL−1 160 μg mL−1 Nikolic et al. (2014)
Eucalyptus citriodora 80 μg mL−1 80 μg mL−1 Nikolic (2015)
Melaleuca 130 μg mL−1 130 μg mL−1 Nikolic (2015)
quinquernervia
Rosa centifolia 130 μg mL−1 130 μg mL−1 Nikolic (2015)
Syzygium aromaticum 130 μg mL−1 130 μg mL−1 Nikolic (2015)
Hyssopus officinalis 160 μg mL−1 160 μg mL−1 Nikolic et al. (2016)
Rosmarinus officinalis 160 μg mL−1 160 μg mL−1 Nikolic et al. (2016)
Pelargonium 250 μg mL−1 250 μg mL−1 Nikolic (2015)
graveolens
ATCC Trachyspermum 0.06 μL mL−1 1 μL mL−1 Mahboubi and
strains copticum Kazempour (2011)
Satureja hortensis 0.125 μL mL−1 2 μL mL−1 Mahboubi and
Kazempour (2011)
Satureja khuzestanica 0.125 μL mL−1 0.125 μL mL−1 Zomorodian et al. (2015)
Zataria multiflora 0.125 μL mL−1 0.25 μL mL−1 Zomorodian et al. (2015)
Carum copticum 0.25 μL mL−1 0.25 μL mL−1 Zomorodian et al. (2015)
Satureja bachtiarica 0.25 μL mL−1 0.5 μL mL−1 Zomorodian et al. (2015)
Salvia mirzayanii 0.5 μL mL−1 0.125 μL mL−1 Zomorodian et al. (2015)
Ocimum sanctum 0.5 μL mL−1 0.25 μL mL−1 Zomorodian et al. (2015)
Artemisia sieberi 4 μL mL−1 1 μL mL−1 Zomorodian et al. (2015)
220 J. Marinković et al.
Analysis of the MIC values revealed the following major observations with
regard to differences in response of tested pathogens to selected EOs:
1. S. sanguinis was more sensitive than S. salivarius on EOs of S. montana, S.
mirzayanii, O. sanctum, and A. sieberi; their major constituent was 1,8-cineole
whose content ranged from 20.8% to 49.3%.
2. S. salivarius was more sensitive than S. sanguinis on EOs of T. vulgare, T. copti-
cum, S. hortensis, Z. multiflora, and S. bachtiarica EOs; their major constituent
was thymol whose content ranged from 28.0% to 49.1%.
3. Interestingly, none of the above mentioned four1,8-cineole-rich EOs also con-
tained thymol nor the other five thymol-rich EOs also contained 1,8-cineole.
Comparing to S. sanguinis, the two cineole-rich oils, S. Montana (49.3%) and O.
sanctum (20.8%), showed about 2 times higher MIC values against S. salivarius,
while those from S. mirzayanii and A. sieberi with similar cineole content (41.2% and
21.1%, respectfully) showed about 4 times higher MIC values. These observations
suggest that the difference in susceptibility between the two Streptococcus species is
not only due to their cineole content; it would be wiser to assume that the cineole-rich
oils lacking thymol are more efficient against S. sanguinis than S. salivarius. The
exact sensitivity ratio to the oils between the two pathogens has to be precisely deter-
mined as it could be useful not only for their possible practical implementation but
also for avoiding undesired defects (Nikolic 2015; Zomorodian et al. 2015).
Similar observation was noticed in the group of even more efficient five EOs; all
of them were thymol-rich but had no 1,8-cineole content. Regardless of their vari-
ability in thymol content (T. vulgare 49.1%, Z. multiflora 37.8%, and S. bachtiarica
28.0%), their MIC values revealed that S. sanguinis was always about twice more
sensitive than S. salivarius. In case of other two thymol-rich oils (T. copticum 45.9%
and S. hortensis 28.2%), the observed sensitivity pattern between the two
Streptococci was even more pronounced (S. sanguinis was about 17 and 16 times
more sensitive to these two oils, respectively). Comparative analysis confirmed that
the thymol-rich oils are highly efficient against the two pathogenic oral Streptococcus
species, so determination of their exact sensitivity ratio to the oils would be crucial
due to many practical reasons (Nikolic et al. 2014; Mahboubi and Kazempour 2011;
Zomorodian et al. 2015).
7.4.2 D
ifferences Between Clinical and Referent ATCC Strains
of Streptococcus sanguinis and Streptococcus salivarius
in Their Sensitivity to Essential Oils
The best efficacy toward S. sanguinis (ATCC 10556) exhibited EOs from Salvia
mirzayanii and Satureja khuzestanica, both with MIC = 0.125 μL mL−1 (Zomorodian
et al. 2015), while the most efficient against corresponding clinical isolates was the
EO of Thymus serpyllum with MIC = 3 μg mL−1 (Nikolic et al. 2014); comparison
7 Outstanding Efficacy of Essential Oils Against Oral Pathogens 221
of efficacy of these two EOs revealed that clinical isolates required 24 times higher
MIC then their corresponding referent ATCC strain (Table 7.1).
Similar pattern in sensitivity between the clinical isolates and corresponding ref-
erent strain was observed for S. salivarius; the most efficient oil against the referent
strain was Trachyspermum copticum with MIC = 0.06 μL mL−1 (Zomorodian et al.
2015), which proved to be about 50 times lower than the most efficient against cor-
responding clinical isolates, the oil of Thymus serpyllum with MIC = 3 μg mL−1
(Nikolic et al. 2014) (Table 7.1).
The least efficient EOs included in this study, with MIC = 250 μg mL−1, were
Pelargonium graveolens against the clinical isolates of both pathogens, as well as
Cymbopogon martinii against the S. sanguinis isolate (Nikolic 2015) and Cyperus
articulatus EO against S. sanguinis ATCC 10556 (Bersan et al. 2014), while the
least efficient on S. salivarius ATCC 9222 was Artemisia sieberi EO, with a quite
lower MIC = 4 μL mL−1 (Zomorodian et al. 2015).
Comparison of selected results confirmed that antimicrobial activity of selected
EOs is statistically different between oils tested on clinical and ATCC strain types,
implicating that the clinical isolates of both Streptococcus species generally show
lower sensitivity to EOs than their corresponding referent strains (Table 7.2).
From the total EOs tested, 39% achieved strong antimicrobial activity toward
ATCC 10556 and ATCC 9222 strains, while in case of clinical isolates, that percent-
age was only 9.3%.
Although Becerril et al. (2012) reported no significant differences in sensitivity,
between the clinical isolates and referent strains of some gram-negative bacteria (E.
coli, S. marcescens, M. morganii, P. mirabilis, and P. aeruginosa) to the activity of
Origanum vulgaris and Cinnamomum zeylanicum EOs, data summarized in our
study show the opposite trend, regardless the strength of the achieved antimicrobial
activity (Table 7.2). The most efficient EOs toward the both referent strains (S. san-
guinis and S. salivarius) had MIC values several times lower (Zomorodian et al.
2015; Mahboubi and Kazempour 2011) than those of their corresponding clinical
isolates (Nikolić et al. 2014), which is also confirmed in our other study on E. fae-
calis (Bogojevic et al. 2016).
As it is quite known that the clinical isolates between themselves may vary in
their sensibility toward any tested agent, and that testing on the referent strains
revealed that they are quite different in sensitivity than the clinical ones, any con-
clusion based on results on either of them separately may bring us to a dead end.
Table 7.2 Response of two Streptococcus spp. strain types to selected antimicrobial essential oils
(EOs)
Antimicrobial activity of EOs
(X ± SD) (Med; min-max)/n (%)
Parameters Strong Good Statistical significance
Strain type ATCC strain 32 (39.0%) 50 (61.0%) p = 0.000a,b
Clinical isolates 16 (9.3%) 156 (90.7%)
a
Chi square test
b
Statistically significant
222 J. Marinković et al.
On the other hand, results that came out from simultaneous testing on both, the
clinical isolates and corresponding referent strains, and application of the same
agent (i.e., essential oil of a known chemical composition) would be more confident
and useful for further studies. Since, to the best of our knowledge, there were no
reports on simultaneous testing of facultative anaerobes such as Streptococcus spp.,
with EOs of presented chemical composition, once again we propose simultaneous
testing procedure with regard to their antimicrobial activity, expressing results on
the ATCC strains just as a referent point in comparison.
7.4.3 S
ome Other Parameters Influencing Essential Oils
Efficacy Toward Two Streptococcus spp.
Further statistical analysis explains weather the content of EO constituents and the
chemical class to which they belong to (the terpene class) influence antimicrobial
activity of EOs (Tables 7.3 and 7.4).
Content of EO constituents is statistically different between strong and good EOs
tested on both ATCC strains and clinical isolates. Strong EOs in general had higher
constituent amount compared with good EOs (Table 7.3).
Apart from the previously described influence of the strain types (clinical isolate/
referent strain) and the amount of the oil component (Fig. 7.5), the chemical class
(terpenes) to which components chemically corresponds was also analyzed as pos-
sible influencing parameter (Table 7.4).
Chemical class, to which EOs constituents chemically belong to, statistically
showed no difference between the groups of EOs with strong and with good antimi-
crobial efficacy tested on both ATCC strains and clinical isolates (Table 7.4).
Logistic regression analysis was used for identification of parameters that may
predict differences between EOs with strong and good antimicrobial activity.
Logistic regression analysis showed influence of EOs, content of single oil con-
stituents, strain type, and chemical class to which oil constituents belong to, on
antimicrobial activity of selected EOs tested toward two Streptococcus spp. When
univariate predictors (Table 7.5) were introduced in a multivariate model, the
Table 7.3 Influence of the amount of EO constituent (%) on antimicrobial activity of tested EOs
towards two Streptococcus spp.
EO antimicrobial efficacy (MIC
values)
(X ± SD) (Med; min-max)
Parameters Strong Good Statistical significance
Content of EO constituent (%) 15.30 ± 16.69 8.74 ± 14.22 p = 0.003a,b
(10.86; (3.25;
1.00–87.70) 1.00–83.10)
a
Mann–Whitney test
b
Statistically significant
7 Outstanding Efficacy of Essential Oils Against Oral Pathogens 223
Table 7.4 Influence of the chemical class to which components belong to (terpene class) on
antimicrobial activity of selected EOs tested towards two Streptococcus spp.
Antimicrobial activity of EOs
Chemical class (terpenes) (%) Strong Good Statistical significancea
Monoterpene hydrocarbons 19 (26.8%) 52 (73.2%) 0.075
Oxygenated monoterpenes 23 (19.3%) 96 (80.7%)
Sesquiterpene hydrocarbons 5 (10.9%) 41 (89.1%)
Oxygenated sesquiterpenes 1 (5.6%) 17 (94.4%)
Chi square test
a
Fig. 7.5 Constituent amount impact on antimicrobial activity of tested EOs toward two
Streptococcus spp
amount of the oil constituents, together with the strain types and classes to which
components chemically belong, proved to be predictor of difference. Results
implicate that higher amounts of constituents obtain better antimicrobial efficiency.
The strain type as a predictor of difference may be explained with a fact that antimi-
crobial efficacy of oils tested on ATCC strains was seven times stronger than the
efficiency on clinical isolates (Table 7.6). Also, the EOs rich in monoterpene hydro-
carbons were the most efficient ones, while the less efficient were EOs with pre-
dominating content of oxygenated sesquiterpenes (Table 7.6).
224 J. Marinković et al.
Table 7.5 Logistic regression analysis of the impact of observed factors on antimicrobial activity
of selected EOs tested towards two Streptococcus spp.
Logistic regression analysis
Uni- Multi-
Parameters expB (95% C.I.) p expB (95% C.I.) p
EO 0.983 (0.951–1.017) 0.327 / /
Components amount 0.976 (0.959–0.994) 0.009a 0.979 (0.959–0.999) 0.040a
Strain type 6.240 (3.163–12.310) 0.000a 6.801 (3.341–13.845) 0.000a
Class of terpenes 1.728 (1.140–2.620) 0.010a 1.984 (1.259–3.126) 0.003a
a
Statistically significant
Table 7.6 Influence of the chemical classes of EOs on the type of Streptococcus spp. strains
(clinical isolate/referent strain)
Antimicrobial power of EO
Referent ATCC strain Clinical isolate
Chemical class/terpene class (%) Strong Good Strong Good
Monoterpene hydrocarbons 12 (50%) 12 (50%) 7 (14.9%) 40 (85.1%)
Oxygenated monoterpenes 16 (50%) 16 (50%) 7 (8.0%) 80 (92.0%)
Sesquiterpene hydrocarbons 3 (16.7%) 15 (83.3%) 2 (7.1%) 26 (92.9%)
Oxygenated sesquiterpenes 1 (12.5%) 7 (87.5%) 0 (0.0%) 10 (100.0%)
Statistical significance p = 0.030a p = 0.379
a
Statistically significant
7.4.4 S
ingle Essential Oil Constituents Influencing Efficacy
Toward Two Streptococcus spp.
If we want to get a general clue what would be the most favorable composition of
EO of the outstanding efficacy on both Streptococcus spp., we might decide to study
them just on the referent strains, as in case of clinical isolates there is only one MIC-
strong EO (Thymus serpyllum) tested against both clinical isolates of targeted oral
pathogens that fulfilled pre-set criteria (Table 7.1). However, it is interesting to
underline that thymol was common constituent in both, the MIC-strong and the
MIC-good oils, and always with high contribution (28–65%), implicating it does
play certain role, possibly in controlled interaction with other components.
Monoterpenoides (monoterpene hydrocarbons and oxygenated monoterpenes),
present in all EOs effective against referent strains of both oral pathogens, are pre-
sented in Table 7.9.
Analysis of data for MIC-strong group of EOs revealed that, within the class of
monoterpene hydrocarbons, we should look for the presence of myrcene, α-thujone,
α-phellandrene and o-cymene, while within the oxygenated monoterpenes, that
would be camphor, 1,8-cineole, carvacrol, eugenol and linalyl acetate. γ-terpinene,
p-cymene and thymol are common in all EOs, regardless they belongs to MIC-
strong or MIC-good group of EOs; their interaction with the above mentioned
9 constituents should be a subject of a further investigation.
7 Outstanding Efficacy of Essential Oils Against Oral Pathogens 227
Camphor. This constituent, while isolated from EO Artemisia feddei, alone exhib-
ited low antimicrobial activity (12.8 mg mL−1) against S. sanguinis ATCC 10556
(Cha et al. 2007), while when isolated from the oil of R. officinalis, it showed to be
quite stronger (400 μg mL−1) against both referent strains, S. salivarius ATCC 25975
and S. sanguinis ATCC 10556 (Bernardes et al. 2010). Differences in antimicrobial
activity on S. sanguinis ATCC 10556 strain, together with the high MIC values,
comparing to the MIC values of selected EOs in our study, again implicate on hid-
den synergistic interactions of camphor with other constituents present in the oils.
7.5 Conclusions
Study on essential oils with pronounced antimicrobial efficacy against target oral
pathogens, S. sanguinis and S. salivarius, associated to standard endodontic proce-
dure obstacles lead us to several statements we would like to outline.
The essential oils rich in 1,8-cineole but lacking thymol are more efficient against
S. sanguinis then S. salivarius, while S. salivarius proved to be more sensitive than
S. sanguinis on thymol-rich oils lacking cineole.
Clinical isolates of both Streptococcus species generally show lower sensitivity
to EOs than their corresponding referent strains.
7 Outstanding Efficacy of Essential Oils Against Oral Pathogens 229
References
Berkiten M, Okar I, Berkiten R (2000) In vitro study of the penetration of Streptococcus sanguis
and Prevotella intermedia strains into human dentinal tubules. J Endod 26:236–239
Bernardes WA, Lucarini R, Tozatti MG et al (2010) Antibacterial activity of the essential oil from
Rosmarinus officinalis and its major components against oral pathogens. Z Naturforsch C
65:588–593
Bersan SMF, Galvao LCC, Goes VFF et al (2014) Action of essential oils from Brazilian native
and exotic medicinal species on oral biofilms. BMC Complement Altern Med 14:451
Bogojevic J, Nikolic M, Markovic T, Ciric A, Markovic D (2016) Analysis of chemical composi-
tion of the most efficient essential oils towards Enterococcus faecalis referent strain ATCC
29212 and clinical isolates. Med Raw Mater 36:3–25
Borges MCL, Sesso MLT, Roberti LR, Oliveira M, Nogueira RD, Geraldo-Martins VR, Ferriani
VP (2015) Salivary antibody response to streptococci in preterm and fullterm children: a pro-
spective study. Arch Oral Biol 60:116–125
Burton JP, Drummond BK, Chilcott CN, Tagg JR, Thomson WM, Hale JDF, Wescombe PA (2013)
Influence of the probiotic Streptococcus salivarius strain M18 on indices of dental health in
children: a randomized double-blind, placebo-controlled trial. J Med Microbiol 62:875–884
Carlsson J, Edlund MB (1987) Pyruvate oxidase in Streptococcus sanguis under various growth
conditions. Oral Microbiol Immunol 2:10–14
Carlsson J, Iwami Y, Yamada T (1983) Hydrogen peroxide excretion by oral Streptococci and
effect of lactoperoxidase–thiocyanate–hydrogen peroxide. Infect Immun 40:70–80
Caufield PW, Dasanayake AP, Li Y, Pan Y, Hsu J, Hardin JM (2000) Natural history of Streptococcus
sanguinis in the oral cavity of infants: evidence for a discrete window of infectivity. Infect
Immun 68:4018–4023
Cha JD (2007) Chemical composition and antibacterial activity against oral bacteria by the essen-
tial oil of Artemisia iwayomogi. J Bacteriol Virol 37:129–136
Cha JD, Jung EK, Kil BS, Lee AY (2007) Chemical composition and antibacterial activity of
essential oil from Artemisia freddei. J Microbiol Biotechnol 17:2061–2065
Chen YY, Clancy KA, Burne RA (1996) Streptococcus salivarius urease: genetic and biochemical
characterization and expression in a dental plaque streptococcus. Infect Immun 64:585–592
CLSI (2013) Performance standards for antimicrobial susceptibility testing; twenty-third infor-
mational supplement. CLSI document M100-S23. Clinical and Laboratory Standards Institute,
Wayne
Cosentino S, Tuberoso CI, Pisano B, Satta M, Mascia V, Arzedi E et al (1999) In-vitro antimicro-
bial activity and chemical composition of Sardinian Thymus essential oils. Lett Appl Microbiol
29:130–135
Crevelin EJ, Caixeta SC, Dias H, Groppo M, Cunha WR, Martins CHG, Crotti AEM (2015)
Antimicrobial activity of the essential oil of Plectranthus neochilus against cariogenic bacteria.
Evid Based Complement Alternat Med 2015:102317
Daniels S (2015) Health Canada approves BLIS K12 Probiotic. Nutraingredients-usa.com; http://
www.nutraingredients-usa.com/Suppliers2/Health-Canada-approves-BLIS-K12-probiotic. 1
Sep 2017
Delgado B, Fernandez PS, Palop A, Periago PM (2004) Effect of thymol and cymene on Bacillus
cereus vegetative cells evaluated through the use of frequency distributions. Food Microbiol
21:327–334
Di Filippo S, Delahaye F, Semiond B et al (2006) Current patterns of infective endocarditis in
congenital heart disease. Heart 92:1490–1495
Di Pierro F, Colombo M, Zanvit A, Rottoli AS (2016) Positive clinical outcomes derived from
using Streptococcus salivarius K12 to prevent streptococcal pharyngotonsillitis in children: a
pilot investigation. Drug Healthc Patient Saf 8:77–81
Didry N, Dubreuil L, Pinkas M (1994) Activity of thymol, carvacrol, cinnamaldehyde and eugenol
on oral bacteria. Pharm Acta Helv 69:25–28
Dorman HJD, Deans SG (2000) Antimicrobial agents from plants: antibacterial activity of plant
volatile oils. J Appl Microbiol 88:308–316
7 Outstanding Efficacy of Essential Oils Against Oral Pathogens 231
Fouad AF, Kum K-Y, Clawson ML, Barry J, Abenoja C, Zhu Q, Caimano M, Radolf JD (2003)
Molecular characterization of the presence of Eubacterium spp. and Streptococcus spp. in end-
odontic infections. Oral Microb Immunol 18:249–255
Fujimura S, Nakamura T (1979) Sanguicin, a bacteriocin of oral Streptococcus sanguis. Antimicrob
Agents Chemother 16:262–265
Gajan EB, Aghazadeh M, Abashov R, Milani AS, Moosavi Z (2009) Microbial flora of root canals
of pulpally-infected teeth: Enterococcus faecalis a prevalent species. J Dent Res Dent Clin
Dent Prospects 3:24–27
Ge Y, Caufield PW, Fisch GS, Li Y (2008) Streptococcus mutans and Streptococcus sanguinis
colonization correlated with caries experience in children. Caries Res 42:444–448
Gomes B, Pinheiro ET, Gade-Neto CR et al (2004) Microbiological examination of infected dental
root canals. Oral Microbiol Immunol 19:71–76
Gregori G, Righi O, Risso P et al (2016) Reduction of group A beta-hemolytic streptococcus
pharyngo-tonsillar infections associated with use of the oral probiotic Streptococcus salivarius
K12: a retrospective observational study. Ther Clin Risk Manag 12:87–92
Heng NC, Haji-Ishak NS, Kalyan A et al (2011) Genome sequence of the bacteriocin producing
oral probiotic Streptococcus salivarius strain M18. J Bacteriol 193:6402–6403
Herrero ER, Slomka V, Bernaerts K et al (2016) Antimicrobial effects of commensal oral species
are regulated by environmental factors. J Dent 47:23–33
Hyink O, Wescombe PA, Upton M, Ragland N, Burton JP, Tagg JR (2007) Salivaricin A2 and the
novel lantibiotic salivaricin B are encoded at adjacent loci on a 190-kilobase transmissible
megaplasmid in the oral probiotic strain Streptococcus salivarius K12. Appl Environ Microbiol
73:1107–1113
Jacinito RC, Gomes BP, Ferraz CC, Zaia AA, Filho FJ (2003) Microbiological analysis of infected
root canal from symptomatic and asymptomatic teeth with periapical periodontitis and the
antimicrobial susceptibility of some isolated anaerobic bacteria. Oral Microbiol Immunol
18:285–292
Juliano C, Mattana A, Usai M (2000) Composition and in vitro antimicrobial activity of the essen-
tial oil of Thymus herba-barona Loisel growing wild in Sardinia. J Essent Oil Res 12:516–522
Juven BJ, Kanner J, Schved F, Weisslowicz H (1994) Factors that interact with the antibacterial
action of thyme essential oil and its active constituents. J Appl Bacteriol 76:626–631
Kaci G, Goudercourt D, Dennin V et al (2014) Anti-inflammatory properties of Streptococcus sali-
varius, a commensal bacterium of the oral cavity and digestive tract. Appl Environ Microbiol
80:928–934
Kadowaki M, Hashimoto M, Nakashima M, Fukata M, Odashiro K, Uchida Y, Shimono N (2013)
Radial mycotic aneurysm complicated with infective endocarditis caused by Streptococcus
sanguinis. Intern Med 52:2361–2365
Kaneko F, Togashi A, Saito S et al (2011) Behcet’s disease (Adamantiades-Behcet’s disease). Clin
Dev Immunol 2011:681956
Kao YT, Shih CM, Tsao NW, Lin FY, Chang NC, Huang CY (2013) Subacute bacterial endocardi-
tis presenting as left upper quadrant abdominal pain. J Chin Med Assoc 76:521–523
Kreth J, Merritt J, Shi W, Qi F (2005) Competition and coexistence between Streptococcus mutans
and Streptococcus sanguinis in the dental biofilm. J Bacteriol 187:7193–7203
Kreth J, Zhang Y, Herzberg MC (2008) Streptococcal antagonism in oral biofilms: Streptococcus
sanguinis and Streptococcus gordonii interference with Streptococcus mutans. J Bacteriol
190:4632–4640
Lambert RJW, Skandamis PN, Coote PJ, Nychas GJE (2001) A study of the minimum inhibi-
tory concentration and mode of action of oregano essential oil, thymol and carvacrol. J Appl
Microbiol 91:453–462
Laura DM, Quinones A, Benenstein R et al (2014) Giant nonfamilial left atrial myxoma presenting
with eye embolism and nonvalvular Streptococcus sanguinis endocarditis. J Am Coll Cardiol
63:2049
Lew HP, Quah SY, Lui JN, Bergenholtz G, Yu VSH, Tan KS (2015) Isolation of alkaline-tolerant
bacteria from primary infected root canals. J Endod 41:451–456
232 J. Marinković et al.
Li J, Helmerhorst EJ, Leone CW, Troxler RF et al (2004) Identification of early microbial colonizers
in human dental biofilm. J Appl Microbiol 97:1311–1318
Lysakowska ME, Ciebiada-Adamiec A, Sienkiewicz M, Sokolowski J, Banaszek K (2016) The
cultivable microbiota of primary and secondary infected root canals, their susceptibility to
antibiotics and association with the signs and symptoms of infection. Int Endod J 49:422–430
Mahboubi M, Kazempour N (2011) Chemical composition and antimicrobial activity of Satureja
hortensis and Trachyspermum copticum essential oil. Iran J Microbiol 3:194–200
Mancl AK, Kirsner SR, Ajdic D (2013) Wound biofilms: lessons learned from oral biofilms.
Wound Repair Regen 21:352–362
Marković T (2011) Etarska ulja i njihova bezbedna primena. Institut za proučavanje lekovitog bilja
„dr Josif Pančić“, Beograd (in Serbian)
Masdea L, Kulik EM, Hauser-Gerspach I, Ramseier AM, Filippi A, Waltimo T (2012) Antimicrobial
activity of Streptococcus salivarius K12 on bacteria involved in oral malodour. Arch Oral Biol
57:1041–1047
Mashima I, Nakazawa F (2014) The influence of oral Veillonella species on biofilms formed by
Streptococcus species. Anaerobe 28:54–61
Matsuo T, Shirakami T, Ozaki K, Nakanishi T, Yumoto H, Ebisu S (2003) An immunohistologic al
study of the localization of bacteria invading root pulpal walls of teeth with periapical lesions.
J Endod 29:194–200
McCarthy C, Snyder M, Parker RB (1965) The indigenous oral flora of man. The newborn to the
1-year old infant. Arch Oral Biol 10:61–70
Nikolić M (2015) Biological activity of selected essential oils towards Staphylococcus,
Streptococcus, Lactobacillus, Pseudomonas, Enterococcus and Candida species isolated from
human oral cavity. Ph.D. Thesis Dissertation, Faculty of Biology, University of Belgrade
Nikolic M, Glamoclija J, Ferreira I et al (2014) Chemical composition, antimicrobial, antioxi-
dant and antitumor activity of Thymus serpyllum L., Thymus algeriensis Boiss. and Reut and
Thymus vulgaris L. essential oils. Ind Crop Prod 52:183–190
Nikolić M, Marković T, Marković D, Glamočlija J, Ćirić A, Smiljković M, Soković M (2016)
Antimicrobial activity of three Lamiaceae essential oils against common oral pathogens. Balk
J Dent Med 20:160–167
Ohnishi Y, Kubo S, Ono Y et al (1995) Cloning and sequencing of the gene coding for dextranase
from Streptococcus salivarius. Gene 156:93–96
Okahashi N, Nakata M, Sakurai A et al (2010) Pili of oral Streptococcus sanguinis bind to fibro-
nectin and contribute to cell adhesion. Biochem Biophys Res Commun 391:1192–1196
Perez F, Calas P, de Falguerolles A, Maurette A (1993) Migration of a Streptococcus sanguis strain
through the root dentinal tubules. J Endod 19:297–301
Power DA, Burton JP, Chilcott CN, Dawes PJ, Tagg JR (2008) Preliminary investigations of the
colonization of upper respiratory tract tissues of infants using a pediatric formulation of the
oral probiotic Streptococcus salivarius K12. Eur J Clin Microbiol Infect Dis 27:1261–1263
Provenzano JC, Rocas IN, Tavares LFD, Neves BC, Siqueira JF (2015) Short-chain fatty acids
in infected root canals of teeth with apical periodontitis before and after treatment. J Endod
41:831–835
Rocas IN, Siqueira JF (2012) Characterization of microbiota of root canal-treated teeth with post-
treatment disease. J Clin Microbiol 50:1721–1724
Rolph HJ, Lennon A, Riggio MP, Saunders WP, MacKenzie D, Coldero L, Bagg J (2001) Molecular
identification of microorganisms from endodontic infections. J Clin Microbiol 39:3282–3289
Rosan B, Lamont RJ (2000) Dental plaque formation. Microbes Infect 2:1599–1607
Sakamoto M, Siqueira JF Jr, Rôças IN, Benno Y (2007) Bacterial reduction and persistence after
endodontic treatment procedures. Oral Microbiol Immunol 22:19–23
Seow WK, Lam JHC, Tsang AKL, Holcombe T, Bird PS (2009) Oral Streptococcus species in pre-
term and full-term children—a longitudinal study. Int J Paediatr Dent 19:406–411
Shovelton DA (1959) Bacterial invasion of dentine around infected pulp canals: a preliminary
report. Alabama Dent Rev 7:7–12
7 Outstanding Efficacy of Essential Oils Against Oral Pathogens 233
Siqueira JF Jr, Rocas IN (2008) Clinical implications and microbiology of bacterial persistence
after treatment procedures. J Endod 34:1291–1301
Siqueira JF Jr, Rôças IN (2009) Diversity of endodontic microbiota revisited. J Dent Res
88:969–981
Siqueira JF, As I, Paiva SSM, Magalhaes KM, Guimaraes-Pinto T (2007) Cultivable bacteria in
infected root canals as identified by 16S rRNA gene sequencing. Oral Microbiol Immunol
22:266–271
Stauffacher S, Lussi A, Nietzsche S, Neuhaus KW, Eick S (2017) Bacterial invasion into radicular
dentine—an in vitro study. Clin Oral Investig 21:1743
Stingu CS, Eschrich K, Rodloff AC, Schaumann R, Jentsch H (2008) Periodontitis is associated
with a loss of colonization by Streptococcus sanguinis. J Med Microbiol 57:495–499
Tagg JR (2008) Streptococci as effector organism for probiotic and replacement therapy. In:
Versalovic J, Wilson M (eds) Therapeutic microbiology: probiotics and related strategies. ASM
Press, Washington, DC, pp 61–81
Tagg JR, Dierksen KP (2003) Bacterial replacement therapy: adapting “germ warfare” to infection
prevention. Trends Biotechnol 21:217–223
Tatikonda A, Sudheep N, Biswas KP, Gowtham K, Pujari S, Singh P (2017) Evaluation of bacte-
riological profile in the apical root segment of the patients with primary apical periodontitis.
J Contemp Dent Pract 18:44–48
Thenisch NL, Bachmann LM, Imfeld T, Leisebach Minder T, Steurer J (2006) Are mutans strep-
tococci detected in preschool children a reliable predictive factor for dental caries risk? A
systematic review. Caries Res 40:366–374
Ultee A, Gorris LGM, Smid EJ (1998) Bactericidal activity of carvacrol towards the food-borne
pathogen Bacillus cereus. J Appl Microbiol 85:211–218
Ultee A, Bennik MHJ, Moezelar R (2002) The phenolic hydroxyl group of carvacrol is essential for
action against the food-borne pathogen Bacillus cereus. Appl Environ Microbiol 68:1561–1568
Walker GV, Heng NCK, Carne A, Tagg JR, Wescombe PA (2016) Salivaricin E and abundant
dextranase activity may contribute to the anti-cariogenic potential of the probiotic candidate
Streptococcus salivarius JH. Microbiology 162:476–486
Wescombe PA, Hale JD, Heng NC et al (2012) Developing oral probiotics from Streptococcus
salivarius. Future Microbiol 7:1355–1371
Wilson W, Taubert KA, Gewitz M et al (2008) Prevention of infective endocarditis: guidelines
from the American Heart Association. J Am Dent Assoc 139:3S
Wisniewska-Spychala B, Sokalski J, Grajek S et al (2012) Dentigenous infectious foci – a risk
factor of infective endocarditis. Med Sci Monit 18:CR93–CR104
Yang TS, Liou ML, Hu TF, Peng CW, Liu TT (2013) Antimicrobial activity of the essential oil of
Litsea cubeba on cariogenic bacteria. J Essent Oil Res 25(2):120–128
Zheng LY, Itzek A, Chen ZY, Kreth J (2011) Oxygen dependent pyruvate oxidase expression and
production in Streptococcus sanguinis. Int J Oral Sci 3:82–89
Zhu L, Kreth J (2010) Role of Streptococcus mutans eukaryotic-type serine/threonine protein
kinase in interspecies interactions with Streptococcus sanguinis. Arch Oral Biol 55:385–390
Zhu L, Kreth J (2012) The role of hydrogen peroxide in environmental adaptation of oral microbial
communities. Oxid Med Cell Longev 2012:717843
Zomorodian K, Ghadiri P, Saharkhiz MJ et al (2015) Antimicrobial activity of seven essential
oils from iranian aromatic plants against common causes of oral infections. Jundishapur
J Microbiol 8:17766e
Chapter 8
Chemical Diversity and Insecticidal
and Anti-tick Properties of Essential Oils
of Plants from Northeast Brazil
8.1 Introduction
Northeast Brazil, the third largest region of the country, is made up of nine states:
Alagoas (AL), Bahia (BA), Ceará (CE), Maranhão (MA), Paraíba (PB), Pernambuco
(PE), Piauí (PI), Rio Grande do Norte (RN), and Sergipe (SE) (Fig. 8.1). Most of the
Northeast is characterized by scarce rainfall, areas with pronounced spatial-temporal
irregularity, and prolonged drought periods, where the annual accumulated rainfall
is less than 500 mm. Conversely, the East Coast presents rainy climate, with annual
accumulated rainfall greater than 1500 mm. The climatic variability and the diverse
biomes of the region favor the occurrence of significant plant diversity, including
medicinal and aromatic species. Part of this diversity is found in the main
northeastern biome, the Caatinga, where several endemic species occur. The
unfavorable climatic conditions, marked by water scarcity and high temperatures,
might have favored the emergence of better-adapted species, which have improved
their biosynthetic capacity in response to the environment over their evolutionary
process. The essential oils of some species found in the Caatinga have a particularly
different chemical composition from those plants found in regions with more
favorable survival conditions. The availability of these plants, together with the
need to obtain natural products to be used as alternatives to the toxic synthetic
products widely applied to crops—which are harmful to man and the environment—
has made them the subject of research on their biotechnological potentialities. In
this sense, the Federal University of Sergipe (UFS) has been developing studies on
Fig. 8.1 Map of Northeast Brazil and its states, with emphasis on the state of Sergipe
aromatic species from Sergipe and other northeastern states, especially regarding
the chemical diversity of essential oils and their biological activities. The essential
oils are analyzed by a mass GC-MS/FID (QP2010 Ultra, Shimadzu Corporation,
Kyoto, Japan), equipped with an autosampler AOC-20i (Shimadzu). The results
enable developing technologies for the sustainable use of these resources in
agriculture and livestock, focusing on natural pesticides and new cultivars of
bioactive plants.
This chapter describes some knowledge about the chemical diversity of the
essential oil of species from Northeast Brazil, their biotechnological potentialities,
and the main factors that have already been described regarding their chemical
diversity.
8.2 C
hemical Diversity of Essential Oils of Plants
from Northeast Brazil
Essential oils are substances derived from plants’ secondary metabolites. In the
past, they were believed to be primary metabolism by-products. Despite not being
part of the essential units of cells, secondary metabolites perform very specific
functions in plants, being advantageous to them for survival and adaptation to
adverse conditions. These substances are synthesized in complex routes, involving
energy expenditure and several chemical reactions using specific enzymes. Plants
produce a true biosynthetic arsenal with a vast diversity of substances, varying both
between and within the same species. Harborne (1988) states that the diversity of
secondary metabolites is partly justified by the fact that plants cannot move nor
respond to the environment as animals can.
8 Chemical Diversity and Insecticidal and Anti-tick Properties of Essential Oils… 237
Essential oils are constituted mostly by a set of volatile and low molecular weight
substances—the terpenes (mono- and sesquiterpenes) and phenylpropanoids. They
are lipophilic, liquid, and unstable in the presence of light and heat, with characteristic
aroma. They usually present a predominant compound, denominated as major
compound. Species are known to have different chemotypes when the essential oil
of their plants contains different major compounds. The essential oil of some species
is formed by ten or fewer compounds; in other cases, it can be formed by 60 or
more. The literature review “Monoterpene and sesquiterpene synthases and the
origin of terpene skeletal diversity in plants” by Degenhardt et al. (2009) reports
that part of this diversity is due to the ability of monoterpene and sesquiterpene
synthase enzymes to form multiple products. All these characteristics make essential
oils the subject of studies focused on their chemical diversity and potentialities as a
source of useful molecules to humans. Several plants are conserved in the collections
maintained at the Active Germplasm Bank of Medicinal and Aromatic Plants of the
Federal University of Sergipe (Table 8.1), located in the municipality of São
Cristóvão, state of Sergipe (lat. 11°00′S, long. 37°12′W). The following topics
describe a few species that occur in Northeast Brazil and their biotechnological
potentialities.
Lippia gracilis (alecrim de tabuleiro) and Lippia sidoides (alecrim de pimenta) are
shrubby plants endemic to the Caatinga, with quite aromatic leaves. L. gracilis is
mainly found in the states of Bahia, Sergipe, and Piauí, while L. sidoides
predominantly occurs in the states of Ceará, Rio Grande do Norte, Bahia, and
Sergipe. Since 2008, the Federal University of Sergipe has carried out the
conservation of genotypes of these species in field collections, with seven accessions
of L. gracilis and ten accessions of L. sidoides. These plants have a high potential of
use owing to their diverse biological activities and are a good example of native
plants that could become a cultivated species once they receive the appropriate
investments for research and technological development. The essential oils of these
Table 8.1 Species, number of accessions, and biological activities detected in aromatic medicinal
plants maintained at the Active Germplasm Bank of Medicinal and Aromatic Plants of the Federal
University of Sergipe, São Cristóvão, state of Sergipe, Brazil
Species No. of accessions Proven biological properties
Lippia gracilis Schauer 07 Insecticide, acaricide, and fungicide
Lippia sidoides Cham. 10 Insecticide, acaricide, and fungicide
Lippia alba (Mill.) N. E. Brown 48 Insecticide, acaricide, and fungicide
Varronia curassavica Jacq. 42 Antiprotozoal
Eplingiella fruticosa Salzm. ex Benth 23 Insecticide
Croton tetradenius Baill. 40 Bactericide and insecticide
238 A. F. Blank et al.
species are rich in monoterpenes, especially thymol and carvacrol. These compounds
are isomers, structurally differing only by the position of the hydroxyl group of the
aromatic ring. Despite having the same molecular formula (C10H14O) and molecular
weights (150.22 g.mol−1) at room temperature, carvacrol is found in the liquid form
while thymol is crystallized (Nostro and Papalia 2012; Holland et al. 2014). This
chapter will further discuss the differences in their biological activities.
Six of the seven L. gracilis accessions maintained at the Active Germplasm Bank
have carvacrol as major compound; the other one has thymol as major compound.
Conversely, nine accessions of L. sidoides present thymol as the major compound;
the remaining one presents carvacrol as major compound. Therefore, L. gracilis and
L. sidoides contain two well-defined chemotypes—thymol and carvacrol.
Although genetic factors determine the ability to synthesize specific compounds,
some quantitative and qualitative variations may occur due to environmental
factors—such as geographic location and soil and climate characteristics,
agricultural practices, development stage, seasonality, and insect attack—and post-
harvest factors. Each of these factors or the interaction between them will act in a
particular way depending on the species. Younger plants can produce essential oil at
a higher quantity than older plants as they can perform the full biosynthetic activity.
Conversely, older plants may, sometimes, produce more essential oils due to their
mature, fully developed biosynthetic “machinery”. Obtaining essential oils
continuously and with standardized quantitative and qualitative characteristics is
still a challenge. Thus, the knowledge about the effects of genetic and environmental
factors on the essential oil characteristics is fundamental.
In L. gracilis, plant age is a critical factor in essential oil production and can cause
quantitative and qualitative changes in this metabolite (Santos et al. 2016). Four-year-
old plants produce on average twice the amount of essential oil produced by 1-year-
old plants. Regarding the chemical composition, the contents of the major compounds
thymol and carvacrol tend to decrease with plant aging, while the contents of second-
ary compounds—mainly myrcene, p-cymene, 1,8-cineole, and γ-terpinene—tend to
increase. Other factors, such as seasonality and water availability, do not cause sig-
nificant changes in the chemical composition of the essential oil of L. gracilis.
However, a mean increment of 35% in essential oil yield (mL.plant−1) is obtained
when plants are harvested in the dry season. A significant advantage of this species is
the stability of the chemical compounds of their essential oil, regardless of the har-
vesting season or the use or not of artificial irrigation (Cruz et al. 2014).
Significant variations in the content and chemical composition may also occur in
the essential oils of older plants of L. sidoides when compared with younger ones
(Santos et al. 2015). Unlike L. gracilis, the essential oil content of L. sidoides
reduces with plant aging. Eight-year-old plants produce, on average, 18% less
essential oil than 2-year-old plants. Carvacrol content tends to increase while thymol
content tends to decrease in older plants of L. sidoides. Plant age also interferes with
the secondary compounds. The contents of terpinen-4-ol, thymol methyl ether, (E)-
caryophyllene, and caryophyllene oxide may increase or remain the same in older
plants. In older plants, the contents of p-cymene and myrcene may reduce or remain
the same, while the content of γ-terpinene may reduce or increase.
8 Chemical Diversity and Insecticidal and Anti-tick Properties of Essential Oils… 239
The variations in the essential oil contents and the percentages of essential oil
compounds are in fact phenotypic variations of the plant. The phenotypic variation is
the result of the genetic variation between the plants, associated with the effects of
non-genetic factors. Understanding the factors that affect essential oil production can
help in obtaining products with preferable chemical characteristics and at the desired
quantity. In L. sidoides, environmental factors have more influence on the essential oil
content than genetic factors when comparing 2- and 8-year-old plants (Santos et al.
2015). Conversely, genetic factors act more strongly than environmental factors on
thymol, carvacrol, thymol methyl ether, (E)-caryophyllene, and caryophyllene oxide.
Although the different factors mentioned above may have a greater or lesser
influence on the chemical content and essential oil composition of L. gracilis and L.
sidoides, the chemotypes of these species do not vary. Regardless of the culture
conditions, both species will always present the chemotypes thymol and carvacrol.
The Myrtaceae family is found in Australia, Asia, Africa, and America and consists
of a large number of species, distributed within 132 genera. Among the 985 species
of the 23 genera that occur in Brazil, 744 are endemic (Sobral et al. 2012). M. lundi-
ana and M. ovata restrictively occur in only two regions—Northeast and Southeast.
In the state of Sergipe, M. lundiana can be found in the Atlantic forest, shrub rest-
inga, high restinga, and white sand restinga. Several plants of this species occur
mainly in the National Park of Serra de Itabaiana (municipality of Areia Branca). M.
ovata is found in the municipality of Japaratuba, in a sandy vegetation area with
intense anthropic activity. Plants of both species are visually similar, and most of the
times their identification requires a taxonomist specialized in this botanical family.
These two forest species are not conserved at the Active Germplasm Bank of
Medicinal and Aromatic Plants of the Federal University of Sergipe due to their
difficult vegetative propagation. Also, seeds and seedlings do not easily develop
when removed from their natural habitat. However, much information has been
obtained from the study on natural populations of these species. Both plants have
essential oils with high antimicrobial potential owing to their compounds. Some
compounds are present in both M. lundiana and M. ovata.
The essential oils of M. lundiana, found in the National Park of Itabaiana, and M.
ovata, found in the municipality of Japaratuba, have a mean content of 1.10% and
1.76%, respectively, considering the volume of oil obtained from leaves dried at
40 °C for 5 days. The essential oil of M. lundiana is classified into three chemotypes,
based on the most abundant compounds: (1) chemotype nerolic acid + 1,8-cineole;
(2) chemotype 1,8-cineole + citral (neral + geranial); and (3) chemotype
1,8-cineole + isopulegol and/or iso-isopulegol. Conversely, with a higher variability,
the essential oil of M. ovata is classified into six chemotypes: (1) nerolic acid, (2)
linalool + nerolic acid, (3) geraniol, (4) citral (neral + geranial), (5) nerolic
acid + E-nerolidol, and (6) linalool + isopulegol + nerolic acid (Alves et al. 2016;
Sampaio et al. 2016).
Among the major compounds identified in the essential oil of these two species,
nerolic acid [(Z)-3,7-dimethyl-2,6-octadienoic acid] stands out for being a very rare
compound, reported in few aromatic species, whose content ranges from 0.8% to
7.7%. Nevertheless, some plants from natural populations of M. lundiana and M.
ovata from the state of Sergipe have been reported to present 33.75% and 72.11%
of nerolic acid, respectively, being the first report of this chemical compound in the
genus Myrcia. Both species have great potential to control phytopathogenic fungi,
especially those responsible for the post-harvest deterioration in tropical fruits.
Between 2013 and 2015, three patents of antimicrobial products and formulations
developed with the use of the essential oil of M. lundiana and M. ovata were
deposited by the Federal University of Sergipe at the National Institute of Industrial
Property of Brazil. Despite the need for basic studies on the propagation of these
species, both for conservation and large-scale production, the potential of these
species to generate safer plant-based antimicrobial products to be used in freshly
consumed foods is evident.
8 Chemical Diversity and Insecticidal and Anti-tick Properties of Essential Oils… 241
lower water and energy consumption are more expensive and require that several
parameters be established and standardized for each species, such as power and
time in microwave-assisted extraction. In V. curassavica, lower water volume and
longer time lead to higher essential oil contents extracted by hydrodistillation.
Conversely, in microwave-assisted extraction, the time and power must be pre-
adjusted to obtain higher essential oil contents. Also, water is not necessary when
using fresh leaves. In both methods, the sesquiterpene hydrocarbon compounds
tend to decrease—e.g., α-humulene and (E)-caryophyllene—when using long
extraction times and/or high water volumes. Oxygenated compounds, however, tend
to increase under these conditions (Nizio et al. 2018).
The knowledge about the effects of different factors, whether involved or not in
the processing stages, on the chemical compounds of the essential oils of a species
is fundamental since they influence the biological activity of the metabolite. The
essential oil of V. curassavica must contain at least 2.3% α-humulene to be used by
the pharmaceutical industry in the production of herbal medicine for inflammation
treatment (Quispe-Condori et al. 2008).
For being commercially exploited, V. curassavica plants from Southeast and
southern Brazil are well known. However, those from Northeast Brazil might
present other potentialities still to be discovered and exploited, whether for medicinal
or agricultural use. A recent study has confirmed the antiprotozoal potential of the
essential oil of V. curassavica to control fish parasites (Nizio et al. 2017). Several
studies on the insecticide activity of the essential oil of this species have been
conducted, and some of them have shown substantial evidence of its potential to
control insect-pests.
and caryophyllene oxide as major compounds. The second group has 1,8-cineole,
α-pinene, and camphor as major compounds, in addition to those of the first group,
but at lower contents. The insecticidal potential to control leaf-cutting ants was
recently confirmed in a study on the essential oils of plants of the second group
(Silva 2017).
Despite its popular use, scientific studies on E. fruticosa are still scarce. The spe-
cies has been maintained in a field collection of the Federal University of Sergipe,
which enables the study on factors that interfere with the characteristics of its essen-
tial oils and the development of conservation strategies, as the species occurs in
environments with intense anthropic activity. E. fruticosa has excellent potential to
combat insect-pests and be used in the production of human medicine.
Understanding the chemical diversity of the essential oils of species already used
in folk medicine is fundamental as they might present several other potentialities.
Besides providing information on the establishment of genetic conservation and
breeding strategies, the knowledge about the essential oil compounds will be useful
in directing further studies. This chapter will report the insecticidal potential of the
essential oil of H. pectinata to control leaf-cutting ants.
After World War II, more precisely with the emergence of the technologies of the
“Green Revolution,” countless synthetic chemicals were used in agriculture. The
development and use of organoclorine, organophosphorus, and carbamate insecticide
stand out among the novelties of that time. Such products, together with other
technologies, have leapfrogged food production in the face of the high demand of
the postwar period. However, these products have had significant negative impacts
on the environment and human health.
Although several of these active principles have been banned, until today, the
principal control method for various insect-pests is based on synthetic chemicals.
Despite the efficiency and reduction of agricultural damage, the use of these
products excessively and indiscriminately continues to damage the ecosystems.
Therefore, studies on plant-based products aimed at alternative pest-control methods
and the sustainable use of natural resources have gained prominence for being
environmentally safer and less toxic to non-target organisms, including humans.
The essential oils are among the plant metabolites with high potential for use in
agriculture. In addition to medicinal, antimicrobial, antiparasitic, and acaricidal
properties, they also have insecticidal properties, which can cause insect deaths or
affect their development, behavior and reproduction.
The following topics address the activities of some essential oils of plants that
occur in Northeast Brazil on ants, termites, and stored-grain insects.
Ants play an important role in ecosystems, as they act in the processes of nutrient
cycling, soil aeration, and seed dissemination (Galitzki et al. 2013; Leal et al. 2015).
Nevertheless, due to the increased use of areas for cultivation and several other
environmental changes caused by anthropic action, ant species have been considered
as “pests” due to the significant damage caused to crops. Leaf-cutting ants, mainly
those of the genus Atta and Acromyrmex, are considered as pests. These ants cause
direct damage to agricultural and forestry crops mainly for their intense leaf-cutting
activity at any stage of plant development, leading to considerable economic losses.
All the plant material is taken to the subterranean nest to serve as a substrate for the
cultivation of fungi with which they maintain a symbiotic relationship (Buratto
et al. 2012).
Chemical control is the most efficient and commonly used method against leaf-
cutting ants. Moreover, certifying entities are abolishing the use of these products in
pest control of planted forests, aiming at economic viability, environmental balance,
and social safety (Jung et al. 2013; FSC 2016).
246 A. F. Blank et al.
Essential oils and their major compounds have been studied to understand their
potential to control leaf-cutting ants. These substances can be directly used as active
ingredients in formulations, mixed with other active ingredients, or serve as a model
for the synthesis of new molecules, as an alternative to the few commercially
available active principles. Several advantages are attributed to the use of plant-
based products as bioinsecticides when compared with synthetic products. For
instance, they originate from renewable resources, present less or no residual effect
on foods—as they are biodegradable—and have minor harmful effects on non-
target organisms. Moreover, for being composed of complex mixtures of compounds,
the possibility of the emergence of resistant individuals is much lower, which
enables the use of the essential oil for a longer period (Gonzalez et al. 2014).
Studies on the essential oils of medicinal and aromatic species from Northeast
Brazil have been carried out, aiming at knowing the toxicity against species of leaf-
cutting ants. A recent study revealed that the exposure pathway is crucial when
evaluating the toxicity of essential oils. The sensitivity to essential oils also varies
between species of leaf-cutting ants. Considering the essential oil of H. pectinata,
the chemotype calamusenone + caryophyllene oxide was more toxic to Acromyrmex
balzani than the chemotype (E)-caryophyllene + caryophyllene oxide (Feitosa-
Alcantara et al. 2017). The doses required to kill 90% of the ants (lethal doses, LD90)
were 9.84 and 31.16 μg.mg−1, respectively, by contact. The opposite was observed
for the species Atta sexdens rubropilosa, where the chemotype (E)-
caryophyllene + caryophyllene oxide was more toxic than the chemotype
calamusenone + caryophyllene oxide, with LD90 of 11.67 and 25.80 μg.mg−1,
respectively, considering the same exposure pathway. When testing the toxicity of
the same chemotypes by fumigation, for both species, the chemotype
calamusenone + caryophyllene oxide was more toxic, with LC90 of 1.35 and 3.52 μL.
L−1 for A. balzani and A. sexdens, respectively. The chemotype (E)-
caryophyllene + caryophyllene oxide had an LC90 of 6.34 and 6.15 μL.L−1,
respectively. The major compounds (E)-caryophyllene, calamusenone, and
caryophyllene oxide tested individually showed lower toxicity levels against species
of leaf-cutting ants than the essential oils tested by contact. These compounds were
not toxic to the ants when applied by fumigation.
The essential oils of E. fruticosa are also toxic to A. balzani when applied by
fumigation, with LC90 values similar to those of the essential oils of H. pectinata. In
addition to insect deaths, the essential oils and major compounds of E. fruticosa also
change the behavior of A. balzani workers (Silva 2017).
H. pectinata and E. fruticosa are potential raw material sources for the develop-
ment of formulations and bioproducts to be used in the management of leaf-cutting
ants as an alternative to synthetic insecticides.
8 Chemical Diversity and Insecticidal and Anti-tick Properties of Essential Oils… 247
The world population’s diet is primarily based on the consumption of seeds and
grains. Faced with the high demand, billions of tons of cereals, oilseeds, and legumes
are produced worldwide. Nevertheless, significant losses have been recorded, owing
to insects’ attacks to stored grains. These insect-pests feed on the grains, resulting
in grain weight loss, low germination, and increased temperature and moisture in
8 Chemical Diversity and Insecticidal and Anti-tick Properties of Essential Oils… 249
the grain mass. Also, they can contaminate the grains by feces residues and body
fragments and obstruct agricultural machinery (Negrisoli et al. 2013).
These insect-pests are mainly controlled by the use synthetic chemicals applied
by contact, fumigation, or spraying with organophosphates, pyrethroids, and growth
regulators. Despite their effectiveness, these products have led to the selection of
resistant populations after their continuous use, requiring higher doses to achieve
the same efficiency. Thus, in addition to the damage caused to the environment and
the applicators’ health, residues of these products have been found in foods at levels
beyond the limits acceptable for consumption (Li et al. 2013; Wei et al. 2014).
Sitophilus zeamais (Coleoptera: Curculionidae) and Tribolium castaneum
(Coleoptera: Tenebrionidae) are two of the main stored grain pests. S. zeamais,
known as maize weevil, can damage grains both in the field and after storage. This
insect-pest decreases grain weight by over 15%, and losses may reach 60% in
6 months (Sousa and Conte 2013). T. castaneum, known as red flour beetle, is a
cosmopolitan species that usually appears after the occurrence of primary pests.
They can infest peanuts, coffee, cocoa, soybeans, dried fruits, nuts, spices,
cottonseed, and all types of ground cereals, such as bran, animal feed, flour, and
meal (Gallo et al. 2002), causing substantial losses.
The essential oils of L. alba (chemotypes citral and carvone) maintained at the
Active Germplasm Bank of the Federal University of Sergipe and the major
compounds (carvone and citral) are toxic to the insect-pests S. zeamais and T. cas-
taneum (Peixoto et al. 2015a). For these two species, the essential oils of carvone
chemotype were more toxic than those of citral chemotype. The mean doses of citral
and carvone required to kill 90% of S. zeamais were 29.65 and 141.5 μL.L−1, respec-
tively. T. castaneum showed to be more tolerant, especially to the essential oils of
the citral chemotype, whose mean LC90 value was 299.0 μL.L−1. For this species,
the essential oils of carvone chemotypes had a mean LC90 of 33.65 μL.L−1. Similarly,
both for S. zeamais and T. castaneum, the major compound carvone was more toxic
(LC90 of 24.0 and 22.8 μL.L−1) than the compound citral (LC90 38.0 and 40.5 μL.
L−1). Besides being toxic, the essential oil of the carvone chemotype has a repellent
effect on these two species. Conversely, the major compound carvone has a repel-
lency effect only on T. castaneum.
Studies have reported the resistance of S. zeamais populations to synthetic insec-
ticides. Depending on the insect origin, it might be more or less resistant to these
products. Likewise, different levels of sensitivity to essential oils may occur within
the species. This fact has been recently confirmed in a study developed by the
Federal University of Sergipe, using the essential oil of L. sidoides (Oliveira et al.
2017) and S. zeamais populations from different states of Brazil. The toxicity of the
essential oil of L. sidoides and its major compound thymol varied according to the
S. zeamais population. Lethal doses (LD90) ranged from 26.88 to 79.16 μg.mg−1 for
the essential oil and from 41.12 to 172.78 μg.mg−1 for thymol. The time required to
kill 50% of the insects (LT50) ranged from 6.8 to 32.4 h and from 26.3 to 46.4 h for
the essential oil and thymol, respectively. These results evidenced the different
sensitivity levels of S. zeamais populations to these metabolites.
250 A. F. Blank et al.
Essential oils have lipophilic characteristics (low water solubility) and are formed
by volatile substances (low molecular weight). Therefore, some obstacles to the
direct application of these plant-based products still have to be overcome. Some
formulations or “prototypes” have been proposed. However, these formulations
must ensure that the essential oils maintain the toxicity level against the target
organism, even in the presence of other products. Nanoformulations obtained from
the essential oil of L. sidoides presented to be toxic to S. zeamais populations. The
LD90 ranged from 81.72 to 114.23 μg.mg−1 and from 40.55 to 61.04 μg.mg−1 for the
nanoformulations of essential oil and thymol, respectively (Oliveira et al. 2017).
The LD90 values for the nanoformulation of the essential oil were higher than those
previously described. Nonetheless, the nanoformulation has only 18% of essential
oil in its composition. The LD90 values of the nanoformulation of thymol were lower
than that of pure thymol. This fact is explained by the nanoparticles of essential oil
and thymol, which are formed during the obtainment of the nanoformulations,
increasing the contact surface of these substances and consequently potentiating
their toxicity to insects.
The essential oils of L. alba, L. gracilis, and L. sidoides—which occur in
Northeast Brazil and are maintained at the Active Germplasm Bank of Medicinal
and Aromatic Plants of the Federal University of Sergipe—are promising sources
for the development of bioproducts to be used in the management of stored grain
insect-pests.
Fig. 8.2 Selection of resistant ticks (red ticks) population after sequential acaricide use (red
arrow)
microplus but with some variations in the activity levels, based on the genotype used
(Monteiro et al. 2014; Soares et al. 2016). Isolated carvacrol has a higher activity on
R. microplus larvae resistant to pyrethroid and amidinic synthetic compounds than
its isomer thymol (Cruz et al. 2013). Even with the higher activity of isolated
carvacrol when compared with isolated thymol, the genotypes with higher carvacrol
contents are not more active than those with higher thymol content. Therefore, the
major compounds of the different genotypes are not correlated with acaricide
activity (Soares et al. 2016).
The essential oil of L. gracilis has also been reported to present activity on R.
microplus (Cruz et al. 2013; Chagas et al. 2016). R. microplus-resistant strains to
organophosphates are more susceptible to the essential oils of L. gracilis when
compared with ticks sensitive to synthetic compounds. This fact is independent of
the L. gracilis genotype and its major compound thymol or carvacrol (Fig. 8.3)
(Costa-Junior et al. 2016). The isolated monoterpene thymol shows no difference in
the acaricidal activity, according to the susceptibility of the R. microplus population
to the synthetic compounds. However, when exposed to isolated carvacrol,
organophosphate-resistant ticks are 3.2 times more susceptible than organophosphate-
sensitive ticks (Costa-Junior et al. 2016).
One of the greatest challenges to the use of essential oils or monoterpenes to
control parasites is their high volatility of these substances, resulting in the low
residual power of the antiparasitic activity. Pharmaceutical formulations reduce the
volatility of essential oils and terpenes (Pham-Hoang et al. 2013), allowing the
252 A. F. Blank et al.
Fig. 8.3 Efficacy of essential oils of Lippia gracilis genotypes 106 and 201 and their major com-
pounds, thymol and carvacrol, on susceptible (green ticks) and organophosphate-resistant larvae
(red ticks) of Rhipicephalus microplus. (Adapted from Costa-Junior et al. 2016)
residual effect of these substances. Yeast cell wall, a by-product of the alcohol
industry, has been used to reduce the volatility of essential oils and monoterpenes
(Martins 2009). Works have demonstrated the efficiency of the encapsulation of
hydrophobic compounds, such as essential oils and monoterpenes (Pannell 1990;
Normand et al. 2005; Shi et al. 2008; Paramera et al. 2011). Carvacrol, the major
monoterpene of the essential oils of several L. gracilis and L. sidoides genotypes,
showed higher acaricidal activity on R. microplus when encapsulated in yeast cell
wall. This formulation had acaricidal activity up to 50 h after application, whereas
the effect of unencapsulated carvacrol lasted only up to 5 h (Fig. 8.4) (Lima et al.
2017).
As previously described, the essential oils of Lippia alba are formed by several
chemotypes, including carvone and citral. The chemotype citral of the essential oils
of L. alba has higher activity on R. microplus larvae. However, they have no activity
on engorged females (Peixoto et al. 2015b). The chemotype carvone of the essential
oil of L. alba shows no acaricidal activity. The isolated monoterpenes citral and
carvone have acaricidal activity on the larvae, with no difference between them;
however, only citral has efficient acaricidal activity on engorged females of R.
microplus (Peixoto et al. 2015b).
8 Chemical Diversity and Insecticidal and Anti-tick Properties of Essential Oils… 253
Fig. 8.4 Lethal concentration (LC50) and the scanning electron image of carvacrol encapsulated
with yeast cell walls (encap carvacrol) and unencapsulated carvacrol against Rhipicephalus
microplus larvae. *Encapsulated carvacrol was significantly different from unencapsulated
carvacrol at p < 0.05. (Adapted from Lima et al. 2017)
Even the chemotypes that have no acaricidal activity may be useful for tick con-
trol, owing to their repellency power (Jaenson et al. 2005). Essential oils with repel-
lent power may be used to increase the residual period of natural or synthetic
compounds that have only the knock-down effect (Novelino et al. 2007). The
carvone chemotype of the essential oils of L. alba, which presents no activity on
ticks, demonstrated repellency power over R. microplus larvae. Nevertheless, the
repellency power varies among essential oils, and slight differences in the chemical
composition interfere with the repellency activity (Lima et al. 2017).
These results evidence the importance of standardizing the entire essential oil
production chain, from cultivation to post-harvest techniques. Further studies on the
biotechnological bias must be developed by testing formulations for the most
diverse activity to control ticks, aiming at producing a commercial formulation for
tick control.
8.5 Conclusions
Contrary to most people’s beliefs, Northeast Brazil is rich in plant biodiversity. The
vegetation of the Caatinga biome is not as lush as that of other Brazilian biomes.
However, a closer look reveals the biological relevance and peculiar beauty of the
species that occur in the northeastern region. Faced with the adverse conditions
imposed by the semi-arid climate, plants had to develop evolutionary strategies to
overcome these unfavorable circumstances. Perhaps these challenges have made
these species even more fascinating. The medicinal and aromatic species exposed in
this chapter represent a small part of the plant richness found in this region. These
plant resources deserve attention for their relevance and potential of use, especially
of their essential oils. Owing to their biological activities, essential oils of plant
254 A. F. Blank et al.
Acknowledgments The authors thank the Federal University of Sergipe, the Federal University
of Maranhão, CNPq, FAPITEC/SE, CAPES, FINEP, and RENORBIO for their financial support
for the projects.
References
Albuquerque AC, Matias GRRS, Couto AAVO, Oliveira MAP, Vasconcelos A (2012) Urban ter-
mites of Recife, Northeast Brazil (Isoptera). Sociobiology 59:183–188
Almeida-Pereira CS (2017) Diversidade química, genética e atividade antimicrobiana do óleo
essencial de Croton tetradenius Baill. Thesis, Universidade Federal de Sergipe
Alves MF, Nizio DAC, Sampaio TS, Nascimento-Junior AF, Brito FA, Melo JO, Arrigoni-Blank
MF, Gagliardi PR, Machado SMF, Blank AF (2016) Myrcia lundiana Kiaersk native popu-
lations have different essential oil composition and antifungal activity against Lasiodiplodia
theobromae. Ind Crop Prod 85:266–273
Anjos CS (2014) Contribuicão química para os voláteis de acessos e extratos por fluido super-
crítico de Cordia verbenaceae DC. Dissertation, Universidade Federal de Sergipe
Apel MA, Ribeiro VLS, Bordignon SAL, Henriques AT, Poser GV (2009) Chemical compo-
sition and toxicity of the essential oils from Cunila species (Lamiaceae) on the cattle tick
Rhipicephalus (Boophilus) microplus. Parasitol Res 105:863–868
Araújo LX, Novato TPL, Zeringota V, Maturano R, Melo D, Silva BC, Daemon E, Carvalho MG,
Monteiro CMO (2016) Synergism of thymol, carvacrol and eugenol in larvae of the cattle tick,
Rhipicephalus microplus, and brown dog tick, Rhipicephalus sanguineus. Med Vet Entomol
30:377–382
Bacci L, Lima JKA, Araújo APA, Blank AF, Silva IMA, Santos AA, Santos ACC, Alves PB,
Picanço MC (2015) Toxicity, behavior impairment, and repellence of essential oils from pep-
per-rosmarin and patchouli to termites. Entomol Exp Appl 156:66–76
Blank AF, Camêlo LCA, Arrigoni-Blank MF, Pinheiro JB, Andrade TM, Niculau ES, Alves
PB (2015) Chemical diversity in Lippia alba (Mill.) N. E. Brown germplasm. Sci World
J 2015:1–11
Borges LMF, Ferri PH, Silva WJ, Silva WC, Silva JC (2003) In vitro efficacy of extracts of Melia
azedarach against the tick Boophilus microplus. Med Vet Entomol 172:28–31
Borges LMF, Sousa LAD, Barbosa CS (2011) Perspectives for the use of plant extracts to control
the cattle tick Rhipicephalus (Boophilus) microplus. Rev Bras Parasitol Vet 20:89–96
Brandão DS, Mendes ADR, Santos RR, Rocha SMG, Leite GLD, Martins ER (2015) Biologia
floral e sistema reprodutivo da erva-baleeira (Varronia curassavica Jacq.). Rev Bras Plantas
Med 17:562–569
Buratto DA, Cardoso JT, Rolim FA, Filho WR (2012) Avaliação dos danos causados por formigas
cortadeiras do gênero Acromyrmex (Hymenoptera) aos plantios de Pinus taeda no planalto
Sul-Catarinense. Floresta 2:683–690
8 Chemical Diversity and Insecticidal and Anti-tick Properties of Essential Oils… 255
Chagas AC, Oliveira MC, Giglioti R, Santana RC, Bizzo HR, Gama PE, Chaves FC (2016)
Efficacy of 11 Brazilian essential oils on lethality of the cattle tick Rhipicephalus (Boophilus)
microplus. Ticks Tick Borne Dis 7:427–432
Costa-Júnior LM, Miller RJ, Alves PB, Blank AF, Li AY, Pérez de León AA (2016) Acaricidal effi-
cacies of Lippia gracilis essential oil and its phytochemicals against organophosphate-resistant
and susceptible strains of Rhipicephalus (Boophilus) microplus. Vet Parasitol 228:60–64
Cruz EMO, Costa-Junior LM, Pinto JAO, Santos DA, Araujo SA, Arrigoni-Blank MF, Bacci L,
Alves PB, Cavalcanti SCH, Blank AF (2013) Acaricidal activity of Lippia gracilis essential
oil and its major constituents on the tick Rhipicephalus (Boophilus) microplus. Vet Parasitol
195:198–202
Cruz EMO, Pinto JAO, Fontes SS, Arrigoni-Blank MF, Bacci L, Jesus HCR, Santos DA, Alves
PB, Blank AF (2014) Water deficit and seasonality study on essential oil constituents of Lippia
gracilis Schauer germplasm. Sci World J 2014:1–9
Degenhardt J, Köllner TG, Gershenzon J (2009) Monoterpene and sesquiterpene synthases and the
origin of terpene skeletal diversity in plants. Phytochemistry 70:1621–1637
Evans TA, Iqbal N (2015) Termite (order Blattodea, infraorder Isoptera) baiting 20 years after
commercial release. Pest Manag Sci 7:897–906
Feitosa-Alcantara RB (2017) Conservação in vitro, diversidade genética, química e atividade for-
micida de Hyptis pectinata L. Poit. Thesis, Universidade Federal de Sergipe
Feitosa-Alcantara RB, Bacci L, Blank AF, Alves PB, Silva IMDA, Soares CA, Sampaio TS,
Nogueira PCL, Arrigoni-Blank MF (2017) Essential oils of Hyptis pectinata chemotypes: iso-
lation, binary mixtures and acute toxicity on leaf-cutting ants. Molecules 22:1–13
FSC-Forest Stewardship Council. Available online: https://br.fsc.org/pt-br. Accessed 9 Dec 2016.
Galitzki EL, Cereto CE, Scherer KZ (2013) Formigas visitantes de inflorescências de
Actinocephalus polyanthus (Bong.) Sano (Eriocaulaceae). Biotemas 26:75–83
Gallo D, Nakano O, Silveira-Neto S, Carvalho RPL, Batista GC, Berti-Filho E, Parra JRP, Zucchi
RA, Alves SB, Vendramim JD, Marchini LC, Lopes JRS, Omoto C (2002) Entomologia agrí-
cola. FEALQ, Piracicaba
Gomes GA, Monteiro CMO, Senra TO, Zeringota V, Calmon F, Matos RS, Daemon E, Gois RW,
Santiago GM, De Carvalho MG (2012) Chemical composition and acaricidal activity of essen-
tial oil from Lippia sidoides on larvae of Dermacentor nitens (Acari: Ixodidae) and larvae and
engorged females of Rhipicephalus microplus (Acari: Ixodidae). Parasitol Res 111:2423–2430
Gomes GA, Monteiro CMO, Julião LS, Maturano R, Senra TO, Zeringota V, Calmon F, Matos
RS, Daemon E, De Carvalho MG (2014) Acaricidal activity of essential oil from Lippia sidoi-
des on unengorged larvae and nymphs of Rhipicephalus sanguineus (Acari: Ixodidae) and
Amblyomma cajennense (Acari: Ixodidae). Exp Parasitol 137:41–45
Gonzalez MS, Lima BG, Oliveira AFR, Nunes DD, Fernandes CP, Santos MG, Tietbohl LAC,
Mello CB, Rocha L, Feder D (2014) Effects of essential oil from leaves of Eugenia sulcataon
the development of agricultural pest insects. Rev Bras Farmacogn 24:413–418
Gupta A, Agarwal AK, Shukla GS (2000) Effect of quinalphos and cypermethrin exposure on
developing blood-brain barrier: role of nitric oxide. Environ Toxicol Pharmacol 8:73–78
Harborne JB (1988) Introduction to ecology biochemistry. Academic, London
Hernandez LE, Parra DG, Marin AC (1987) Accion repelente y acaricida del Melinis minutiflora
sobre el Boophilus microplus. Rev Colomb Cienc Quim Farm 16:17–21
Holland RD, Wilkes JG, Cooper WM, Alusta P, Williams A, Pearce B, Beaudoin M, Buzatu D
(2014) Thymol treatment of bacteria prior to matrix-assisted laser desorption/ionization time-
of-flight mass spectrometric analysis aids in identifying certain bacteria at the subspecies level.
Rapid Commun Mass Spectrom 28:2617–2626
Hu D, Coats J (2008) Evaluation of the environmental fate of thymol and phenethyl propionate in
the laboratory. Pest Manag Sci 64:775–779
Jaenson TGT, Palsson K, Borg-Karlson AK (2005) Evaluation of extracts and oils of tick-repellent
plants from Sweden. Med Vet Entomol 19:345–352
256 A. F. Blank et al.
Jesus AS, Blank AF, Alves MF, Arrigoni-Blank MF, Lima RN, Alves PB (2016) Influence of stor-
age time and temperature on the chemical composition of the essential oil of Hyptis pectinata
L. Poit. Rev Bras Plantas Med 18:336–340
Jung PH, Silveira AC, Nieri EM, Potrich M, Silva ERL, Refatti M (2013) Atividade Inseticida
de Eugenia uniflora L. e Melia azedarach L. sobre Atta laevigata Smith. Floresta Ambient
20:191–196
Leal IR, Leal LC, Andersen AN (2015) The benefits of myrmecochory: a matter of stature.
Biotropica 47:281–285
Lebouvier N, Hue T, Hnawia E, Lesaffre L, Menut C, Nour M (2013) Acaricidal activity of
essential oils from five endemic conifers of New Caledonia on the cattle tick Rhipicephalus
(Boophilus) microplus. Parasitol Res 112:1379–1384
Li SG, Li MY, Huang YZ, Hua RM, Lin HF, He YJ, Wei LL, Liu ZQ (2013) Fumigant activity
of Illicium verum fruit extracts and their effects on the acetylcholinesterase and glutathione S
-transferase activities in adult Sitophilus zeamais. J Pest Sci 86:677–683
Lima JKA, Albuquerque ELD, Santos ACC, Oliveira AP, Araújo APA, Blank AF, Arrigoni-Blank
MF, Alves PB, Santos DA, Bacci L (2013) Biotoxicity of some plant essential oils against the
termite Nasutitermes corniger (Isoptera: Termitidae). Ind Crop Prod 47:246–251
Lima AS, Maciel AP, Mendonça CJS, Costa-Junior LM (2017) Use of encapsulated carvacrol with
yeast cell walls to control resistant strains of Rhipicephalus microplus (Acari: Ixodidae). Ind
Crop Prod 108:190–194
Martins MS (2009) Leveduras de cerveja e Cana-de-Açúcar (Saccharomyces cerevisiae), autoli-
sada e íntegra, na dieta de cães. Dissertation, Universidade Estadual Paulista, Faculdade de
Ciências Agrárias e Veterinárias
Massard CL, Fonseca AH (2004) Carrapatos e doenças transmitidas, comuns ao homem e aos
animais. A Hora Veterinária 135:15–23
Menezes IAC, Marque MS, Santos TC, Dias KS, Silva ABL, Mello ICM, Lisboa ACCD, Alves PB,
Cavalcanti SCH, Marçal RM, Antoniolli AR (2007) Antinociceptive effect and acute toxicity of
the essential oil of Hyptis fruticosa in mice. Fitoterapia 78:192–195
Monteiro CM, Araújo LX, Gomes GA, Senra TO, Calmon F, Daemon E, de Carvalho MG,
Bittencourt VR, Furlong J, Prata MC (2014) Entomopathogenic nematodes associated with
essential oil of Lippia sidoides for control of Rhipicephalus microplus (Acari: Ixodidae).
Parasitol Res 113:189–195
Negrisoli CRCB, Negrisoli AS Jr, Bernardi D, Garcia MS (2013) Activity of eight strains of
entomopathogenic nematodes (Rhabditida: Steinernematidae, Heterorhabditidae) against five
stored product pests. Exp Parasitol 134:384–388
Nizio DAC, Brito FA, Sampaio TS, Melo JO, Silva FLS, Gagliardi PR, Arrigoni-Blank MF, Anjos
CS, Alves PB, Wisniewski-Junior A, Blank AF (2015) Chemical diversity of native populations
of Varronia curassavica Jacq. and antifungal activity against Lasiodiplodia theobromae. Ind
Crop Prod 76:437–448
Nizio DAC, Fujimoto RY, Maria AN, Carneiro PCF, França CCS, Sousa NC, Brito FA, Sampaio
TS, Arrigoni-Blank MF, Blank AF (2017) Essential oils of Varronia curassavica accessions
have different activity against white spot disease in freshwater fish. Parasitol Res 117:97–105
Nizio DAC, Blank AF, Sampaio TS, Brito FA, Matos TM, Arrigoni-Blank MF, Maria AN (2018)
Distillation methods affect the chemical composition of Varronia curassavica Jacq. essential
oil? Biosci J 34:629–639
Normand V, Dardelle G, Bouquerand PE, Nicolas L, Johnston DJ (2005) Flavor encapsulation in
yeasts: limonene used as a model system for characterization of the release mechanism. J Agric
Food Chem 53:7532–7543
Nostro A, Papalia T (2012) Antimicrobial activity of carvacrol: current progress and future pro-
spectives. Recent Pat Antiinfect Drug Discov 7:28–35
Novelino MAS, Daemon E, Soares GLG (2007) Avaliação da atividade repelente do timol, mentol,
salicilato de metila e ácido salicilico sobre larvas de Boophilus microplus (Canestrini, 1887)
(Acari: Ixodidae). Arq Bras Med Vet Zootec 59:700–704
8 Chemical Diversity and Insecticidal and Anti-tick Properties of Essential Oils… 257
Oliveira AP, Santana AS, Santana EDR, Lima APS, Faro RRN, Nunesb RS, Lima AD, Blank
AF, Araújo APA, Paulo CF, Bacci L (2017) Nanoformulation prototype of the essential oil
of Lippia sidoides and thymol to population management of Sitophilus zeamais (Coleoptera:
Curculionidae). Ind Crop Prod 107:198–205
Pannell NA (1990) Microencapsulation in microorganisms. European Patent EP 0242 135 B1
Paramera EI, Konteles SJ, Karathanos VT (2011) Microencapsulation of curcumin in cells of
Saccharomyces cerevisiae. Food Chem 125:892–902
Passos GF, Fernandes ES, Da Cunha FM, Ferreira J, Pianowski LF, Campos MM, Calixto JB
(2007) Anti-inflammatory and anti-allergic properties of theessential oil and active compounds
from Cordia verbenacea. J Ethnopharmacol 110:323–333
Peixoto MG, Bacci L, Blank AF, Araújo APA, Alves PB, Silva JHS, Santos AA, Oliveira AP,
Costa AS, Arrigoni-Blank MF (2015a) Toxicity and repellency of essential oils of Lippia alba
chemotypes and their major monoterpenes against stored grain insects. Ind Crop Prod 71:1–36
Peixoto MG, Costa-Júnior LM, Blank AF, Lima ADS, Menezes TS, Santos DA, Alves PB,
Cavalcanti SC, Bacci L, Arrigoni-Blank MF (2015b) Acaricidal activity of essential oils
from Lippia alba genotypes and its major components carvone, limonene, and citral against
Rhipicephalus microplus. Vet Parasitol 210:118–122
Pham-Hoang BN, Romero-Guido C, Phan-Thi H, Waché Y (2013) Encapsulation in a natural,
preformed, multi-component and complex capsule: yeast cells. Appl Microbiol Biotechnol
97:6635–6645
Quispe-Condori S, Foglio MA, Rosa PTV, Meireles MAA (2008) Obtaining β-caryophyllene from
Cordia verbenacea de Candolle by supercritical fluid extraction. J Supercrit Fluids 46:27–32
Raymundo LJRP, Guilhon CC, Alviano DS, Matheus ME, Antoniolli AR, Cavalcanti SCH, Alves
PB, Alviano CS, Fernandes PD (2011) Characterization of the anti-inflammatory and antinoci-
ceptive activities of the Hyptis pectinata (L.) Poit essential oil. J Ethnopharmacol 134:725–732
Sampaio TS, Nizio DAC, White LAS, Melo JO, Almeida CS, Alves MF, Gagliardi PR, Arrigoni-
Blank MF, Wisniewski-Junior A, Sobral MEG, Blank AF (2016) Chemical diversity of a wild
population of Myrcia ovata Cambessedes and antifungal activity against Fusarium solani. Ind
Crop Prod 86:196–209
Santos CP, Oliveira TC, Pinto JAO, Fontes SS, Cruz EMO, Arrigoni-Blank MF, Andrade TM,
Matos IL, Alves PB, Innecco R, Blank AF (2015) Chemical diversity and influence of plant
age on the essential oil from Lippia sidoides Cham. germplasm. Ind Crop Prod 76:416–421
Santos CP, Pinto JAO, Santos CA, Cruz EMO, Arrigoni-Blank MF, Andrade TM, Santos DA,
Alves PB, Blank AF (2016) Harvest time and geographical origin affect the essential oil of
Lippia gracilis Schauer. Ind Crop Prod 79:205–210
Santos AA, Oliveira BMS, Melo CR, Lima APS, Santana EDR, Blank AF, Picanço MC, Araújo
APA, Cristaldo PF, Bacci L (2017) Sub-lethal effects of essential oil of Lippia sidoides on
drywood termite Cryptotermes brevis (Blattodea: Termitoidea). Ecotoxicol Environ Saf
145:436–441
Shi G, Rao L, Yu H, Xiang H, Yang H, Ji R (2008) Stabilization of photosensitive resveratrol within
yeast cell. Int J Pharm 349:83–93
Silva JS (2009) O gênero Croton L. (Euphorbiaceae) em Pernambuco, com ênfase nas espécies da
microrregião do Vale do Ipanema. Dissertation, Universidade Federal Rural de Pernambuco
Silva DC (2017) Diversidade química, genética e estudo do potencial formicida do óleo essen-
cial de Eplingiella fruticosa (Salzm. ex Benth.) Harley & J.F.B. Pastore. Thesis, Universidade
Federal de Sergipe
Silva WJ, Dória GAA, Maia RT, Nunes RS, Carvalho GA, Blank AF, Alves PB, Marçal RM,
Cavalcanti SCH (2008) Effects of essential oils on Aedes aegypti larvae: alternatives to envi-
ronmentally safe insecticides. Bioresour Technol 99:3251–3255
Silva DC, Blank AF, Nizio DAC, Sampaio TS, Nogueira PCL, Arrigoni-Blank MF (2018) Chemical
diversity of essential oils from native populations of Eplingiella fruticosa. Crop Breed Appl
Biotechnol 18:205–214
258 A. F. Blank et al.
Soares AM, Penha TA, Araújo SA, Cruz EMO, Blank AF, Costa-Junior LM (2016) Assessment
of different Lippia sidoides genotypes regarding their acaricidal activity against Rhipicephalus
(Boophilus) microplus. Rev Bras Parasitol Vet 25:401–406
Sobral M, Grippa CR, Souza MC, Aguiar OT, Bertoncello R, Guimarães TB (2012) Fourteen new
species and two taxonomic notes on Brazilian Myrtaceae. Phytotaxa 50:19–50
Sousa G, Conte H (2013) Midgut morphophysiology in Sitophilus zeamais Motschulsky, 1855
(Coleoptera: Curculionidae). Micron 51:1–8
Tabari MA, Youssefi MR, Maggi F, Benelli G (2017) Toxic and repellent activity of selected mono-
terpenoids (thymol, carvacrol and linalool) against the castor bean tick, Ixodes ricinus (Acari:
Ixodidae). Vet Parasitol 245:86–91
Verma M, Sharma S, Prasad R (2009) Biological alternatives for termite control: a review. Int
Biodeterior Biodegradation 63:959–972
Wei L, Hua R, Li M, Huang Y, Li S, He Y, Shen Z (2014) Chemical Composition and biological
activity of star anise Illicium verum extracts against maize weevil, Sitophilus zeamais adults.
J Insect Sci 14:1–13
Part III
Extraction and Bioanalytical Techniques
Chapter 9
Adulteration Analysis in Essential Oils
Prabodh Satyal and William N. Setzer
According to Harrewijn et al. (2000), essential oils are complex mixtures of volatile
compounds that are produced by living organisms and isolated by physical means,
such as pressing and distillation from a whole plant or plant portion of known
taxonomic origin. Controversy surrounds the definition of essential oils from the
point of view of distillation (Sadgrove and Jones 2015). Therefore, the ISO
(International Organization for Standardization) has defined a universally accepted
definition for essential oil as “Products obtained from natural raw material, either by
distillation with water and steam, or from the epicarp of citrus fruits by mechanical
processing, or by dry distillation” (Schnaubelt 1999; International Organization for
Standardization 2013).
Essential oils are stored in specialized cells/glandular cells or organelles within
any plant tissue. The first recorded method of extracting essential oils was written
by Andalusian physician and chemist Ibn al-Baitar in the thirteenth century
(Firenzuoli et al. 2014). Modern techniques rely on pressing, rubbing, or heating
particular regions to rupture the cells and release the aromatic compounds.
Therefore, essential oils are mostly obtained by cold pressing, steam distillation,
and hydrodistillation.
The essential oil components are mainly produced through four major biosyn-
thetic pathways: (1) the mevalonate pathway leading to sesquiterpenes and triter-
penes, (2) the methyl-erythritol pathway leading to mono- and diterpenes, (3) the
shikimic acid pathway yielding phenylpropanoids, and (4) the acetate pathway for
fatty acid-derived compounds. With only these pathways, there are still large num-
The formation of synthetic markers can be caused by oxidation (e.g., neric acid as a
synthetic marker for neral), reduction (e.g., dihydro linalool as a synthetic marker of
linalool, dihydro linalyl acetate as a synthetic marker of linalyl acetate (Frey 1988)
(see below)), thermal rearrangement (e.g., citrollene, 2,6-dimethyloctane, and
isolimonene for monoterpene hydrocarbons (Stolle et al. 2009); plinol as a synthetic
marker of linalool and plinyl acetate as a synthetic marker of linalyl acetate, derived
from α-pinene as a natural precursor (see below) (Sell 2003; Leiner et al. 2013)),
intermediate isomerization (e.g., α-linalool (see below) (Radulović et al. 2013)),
acetylated markers (e.g., terpin diacetate as a synthetic marker of terpinyl acetate
(Suga et al. 1983)), and product–reactant interaction markers (e.g., phenylpentadienal
as a synthetic marker of (E)-cinnamaldehyde (see below) (Frey 1988)).
Many individual essential oil components are synthetically accessible with mod-
ern synthetic techniques, but the cost of mass production and the purity of the final
product dictate the selection of synthetic adulterants based on economic viability. In
fact, few sesquiterpenes are synthesized from natural or synthetic precursors. Thus,
despite having large numbers of sesquiterpenoids, adulteration of essential oils with
synthetic or semisynthetic sesquiterpenoids is not generally a problem, so monitor-
ing synthetic markers has not been pursued. However, emerging genetically engi-
neered biosynthetic chemicals are in the process of being industrially produced
(Diaz-Chavez et al. 2013), and thus this will introduce an added level of concern in
the years to come as these processes become more refined and cost effective.
In almost all of the literature reports, the discovered synthetic markers were
found in very minor quantities (<0.5%) in the oils they were used to adulterate.
Therefore, the selected ion monitoring analysis (SIM) method (Frey 1988) should
be used for efficiently detecting synthetic markers in complex adulterated essential
oils.
Linalool and linalyl acetate are the main constituents of several commercial essen-
tial oils, such as coriander (Coriandrum sativum) leaf and seed, ho wood
(Cinnamomum camphora), rosewood (Aniba rosaeodora), lavender (Lavandula
angustifolia), bergamot (Citrus bergamia), lavandin (Lavandula spp.), and clary
sage (Salvia sclarea). Synthetic linalool contains α-linalool, which is produced by
the incomplete acid-catalyzed isomerization of 6-methyl-6-hepten-2-one to
6-methyl-5-hepten-2-one, followed by the base-catalyzed ethynylation and selec-
tive hydrogenation of the resulting mixture, resulting in α-linalool, a synthetic
marker, and β-linalool, the target compound (Fig. 9.1) (Radulović et al. 2013).
Similarly, over-hydrogenation of linalool or linalyl acetate gives rise to dihydro
linalool or dihydro linalyl acetate as synthetic impurities (Fig. 9.1). Synthetic
9 Adulteration Analysis in Essential Oils 265
Fig. 9.1 Generation of the synthetic marker of linalool and linalyl acetate, following a petrochem-
ical-based precursor, 6-methyl-5-hepten-2-one
linalool has also been prepared from α-pinene by pyrolysis of the intermediate
pinane hydrate (Fig. 9.2). Intramolecular ene reaction leads to a mixture of plinols
as synthetic markers, which can be converted to the corresponding plynyl acetates.
Fig. 9.2 Generation of the synthetic marker of linalool and linalyl acetate, following a natural
precursor, α-pinene
The major component of thyme (Thymus vulgaris) and oregano (Origanum vulgare)
essential oils is thymol. Thymol is industrially synthesized from m-cresol as the
starting material by electrophilic aromatic substitution of m-cresol with propene
(Fig. 9.4) to give 6-isopropyl-3-methylphenol (thymol) at 1290 RI on a DB-5 col-
umn as the major, thermodynamically favored, product (Biedermann et al. 1978;
Wimmer et al. 1991). However, a less thermodynamically favored, minor component,
9 Adulteration Analysis in Essential Oils 267
Fig. 9.4 Preparation of thymol from m-cresol, a petroleum-based precursor, as the starting material
Cinnamon (Cinnamomum zeylanicum) bark and cassia (C. cassia) bark essential oils
are often adulterated with synthetic trans-cinnamaldehyde. Synthetic trans-cinnamal-
dehyde is produced by the base-catalyzed aldol condensation of benzaldehyde and
acetaldehyde (Fig. 9.5) (Richmond 1950). In this synthesis, a common impurity is
5-phenylpentadienal, which is confirmed by the presence of 129, 128, and 158 m/z
peaks in the mass spectrum. 5-Phenylpentadienal is formed by the crossed-aldol
reaction of cinnamaldehyde with acetaldehyde (Frey 1988).
Wintergreen (Gaultheria procumbens) and birch (Betula lenta) essential oils are
similarly adulterated by synthetic methyl salicylate, which can be characterized
by the presence of phenol and an isomer of dimethyl-2-hydroxyterephthalate
(Frey 1988). Methyl salicylate is prepared by Kolbe-Schmitt carboxylation of phenol
268 P. Satyal and W. N. Setzer
Nerolidol is a major component in the neroli (Citrus auranthus floral) and nerolina
(Melaleuca quinquenervia) essential oils. Synthetic nerolidol has been prepared
using linalool as a starting material (Fig. 9.7) (Surburg and Panten 2006; Chan et al.
2016). Linalool is reacted with ethyl acetoacetate; the acetoacetic ester interme-
diate undergoes thermal rearrangement with decarboxylation to give (E)- and (Z)-
geranylacetone. Nucleophilic addition of acetylene to the geranylacetones gives
the corresponding (E)- and (Z)-dehydronerolidols, which can be partially hydroge-
nated to give (E)-nerolidol and (Z)-nerolidol. Adulteration with synthetic nerolidol
generally shows trace amounts of dehydronerolidol and geranylacetone.
In the synthesis of organic compounds, 100% pure desired yield is never achieved
since there are always side reactions that occur yielding at least trace by-products.
Identifying them is challenging since they are not thoroughly researched nor even
incorporated into any commercial libraries. However, identifying and characterizing
the peaks of these trace markers is perhaps the best overall strategy of all the
currently available technologies for detecting essential oil adulteration. This work is
270 P. Satyal and W. N. Setzer
perhaps the first significant overview that has been published on the synthetic
markers of essential oil components. Sometimes essential oil components are also
degraded, or the wrong distillation technique is applied, which results in unusual
essential oil components, but synthetic markers are most always distinct from those
arising from unintended degradations (Turek and Stintzing 2013). As knowledge
increases on the synthetic markers in essential oil adulteration, manufacturers of the
various commercially available components are continually developing new ways
to remove the identifying trace markers by using various purification techniques.
Target synthesized compounds and trace markers have similar boiling points and
retention properties, so they present a considerable challenge to current purification
methodologies.
There are two types of precursor molecules used in synthesizing essential oil
components: natural component-based and petrochemical-based. Sometimes,
natural component-based synthesis produces enantioselective products (e.g., the
synthesis of carvone from limonene (Fig. 9.3) (Surburg and Panten 2006)). As a
result, it passes isotopic ratio testing and enantioselective testing. Petrochemical-
based synthetic products, however, do not pass the isotopic ratio analysis.
Petrochemical-based synthetics may also fail enantioselective measurements, but in
some cases, there are no enantiomeric aspects to consider. For example, in the
synthesis of thymol (Fig. 9.4), we see that the thymol molecule has no chiral centers
and thus not amenable to enantioselective testing. With chiral GC-MS being of no
value in this case, it leaves only the issue of isotopic ratios to be concerned about
from an adulteration standpoint. To pass isotopic ratio testing, most of the so-called
natural essential oil producers prefer to use natural precursor-based synthetic
compounds rather than those derived from a petrochemical sources. While the
naturally derived components can be more expensive than petrochemical-based
compounds, oftentimes they are still considerably cheaper than the essential oils
themselves, thus making them very attractive additives. Compounds like α-pinene,
limonene, geraniol, 1,8-cineole (eucalyptol), and citronellal are frequently used as
natural isolates to synthesize nature-identical chemicals (Surburg and Panten 2006),
which would pass isotopic testing.
In many cases the naturally derived precursors are actually less expensive than the
same compounds if they were to be made synthetically. For example, α-pinene is
obtained from turpentine sulfate (a waste product of paper mills), limonene can be
extracted from cheap orange oils (US$5 kg−1), 1,8-cineole (eucalyptol) is extracted
from Eucalyptus essential oil, and citral is extracted from lemongrass essential oils
(Sell 2003). But when the natural precursors are isolated, there are typically charac-
teristic pyrolyzed markers that arise from the isolation process. There are poten-
tially more markers found in natural precursor-based synthetic compounds than in
petrochemical-based synthetic compounds; therefore achieving an acceptable level
of purification of the natural precursors can be challenging unless the precursor can
be crystallized. Trace impurities in the natural precursors are also affected by the
reaction carried out in synthesizing essential oil molecules.
9 Adulteration Analysis in Essential Oils 271
References
Aprotosoaie AC, Hăncianu M, Costache I, Miron A (2014) Linalool: a review on a key odorant mol-
ecule with valuable biological properties. Flavour Fragr J 29:193–219. https://doi.org/10.1002/
ffj.3197
Biedermann W, Koller H, Wedemeyer K (1978) Process for preparing thymol. United States Patent
4,086,283
Bordenca C, Allison RK (1951) l-Carvone from d-limonene. Ind Eng Chem 43(5):1196–1198
Brumer M (2014) The mystery of drugs and perfumed olive oil: “Samim” and “besamim” in
incense and holy anointment oil. J Intercult Interdiscip Archaeol 2014(1):89–102
Carpenter MS, Easter WM (1955) The isopropyl cresols. J Org Chem 20(4):401–411
Chan WK, Tan LTH, Chan KG, Lee LH, Goh BH (2016) Nerolidol: a sesquiterpene alcohol
with multi-faceted pharmacological and biological activities. Molecules 21:529. https://doi.
org/10.3390/molecules21050529
Culp RA, Noakes JE (1990) Identification of isotopically manipulated cinnamic aldehyde and
benzaldehyde. J Agric Food Chem 38:1249–1255
Diaz-Chavez ML, Moniodis J, Madilao LL, Jancsik S, Keeling CI, Barbour EL, Ghisalberti
EL, Plummer JA, Jones CG, Bohlmann J, Cirino PC (2013) Biosynthesis of sandalwood oil:
Santalum album CYP76F cytochromes P450 produce santalols and bergamotol. PLoS One
8:e75053
Do TKT, Hadji-Minaglou F, Antoniotti S, Fernandez X (2015) Authenticity of essential oils.
Trends Anal Chem 66:146–157. https://doi.org/10.1016/j.trac.2014.10.007
Ernst E (2002) Adulteration of Chinese herbal medicines with synthetic drugs: a systematic review.
J Intern Med 252:107–113
Everstine K, Spink J, Kennedy S (2013) Economically motivated adulteration (EMA) of
food: common characteristics of EMA incidents. J Food Prot 76(4):723–735. https://doi.
org/10.4315/0362-028X.JFP-12-399
Firenzuoli F, Jaitak V, Horvath G, Bassolé IHN, Setzer WN, Gori L (2014) Essential oils: new per-
spectives in human health and wellness. Evid Based Complement Alternat Med 2014:467363
Frey C (1988) Detection of synthetic flavorant addition to some essential oils by selected ion
monitoring GC/MS. In: Lawrence BM, Mookherjee BD, Willis BJ (eds) Flavors and fragrance:
a world perspective. Elsevier BV, Amsterdam, pp 517–524
Guenther E (1992) The essential oils, volumes I–VI. Krieger Publishing Company, Malabar
Harrewijn P, van Oosten AM, Piron PGM (2000) Natural terpenoids as messengers. Springer-
Science+Business Media, Dordrecht
Heuberger E, Hongratanaworakit T, Böhm C, Weber R (2001) Effects of chiral fragrances on
human autonomic nervous system parameters and self-evaluation. Chem Senses 26(3):281–292
Höld KM, Sirisoma NS, Ikeda T, Narahashi T, Casida JE (2000) α-Thujone (the active component
of absinthe): γ-aminobutyric acid type A receptor modulation and metabolic detoxification.
Proc Natl Acad Sci U S A 97(8):3826–3831
Huang WF, Wen KC, Hsiao ML (1997) Adulteration by synthetic therapeutic substances of tradi-
tional Chinese medicines in Taiwan. J Clin Pharmacol 37(4):344–350
International Organization for Standardization, ISO (2013) ISO 9235:2013: aromatic natural raw
materials—vocabulary. https://www.iso.org/standard/51017.html. Accessed 28 Sep 2017
Krock KA, Ragunathan N, Wilkins CL (1994) Multidimensional gas chromatography coupled
with infrared and mass spectrometry for analysis of Eucalyptus essential oils. Anal Chem
66(4):425–430
Kubeczka KH (2010) History and sources of essential oil research. In: Başer KHC, Buchbauer G
(eds) Handbook of essential oils. CRC Press, Boca Raton, pp 3–38
Lawrence BM (2002) Commercial essential oils: truths and consequences. In: Swift KAD (ed)
Advances in flavours and fragrances. Royal Society of Chemistry, Cambridge, pp 57–83
Leiner J, Stolle A, Ondruschka B, Netscher T, Bonrath W (2013) Thermal behavior of pinan-2-ol
and linalool. Molecules 18:8358–8375. https://doi.org/10.3390/molecules18078358
272 P. Satyal and W. N. Setzer
Marriott PJ, Shellie R, Cornwell C (2001) Gas chromatographic technologies for the analysis of
essential oils. J Chromatogr A 936:1–22
Merriam-Webster.com (n.d.) Adulterate, Merriam-Webster Dictionary. https://www.merriam-web-
ster.com/dictionary/adulterate. Accessed 25 Sep 2017
NIIR Board (2004) Modern technology of perfumes, flavours and essential oils, 2nd edn. National
Institute of Industrial Research, New Delhi
Patočka J, Dvořák A (2004) Biomedical aspects of chiral molecules. J Appl Biomed 2:95–100
Radulović NS, Blagojević PD, Miltojević AB (2013) α-Linalool – a marker compound of forged/
synthetic sweet basil (Ocimum basilicum L.) essential oils. J Sci Food Agric 93(13):3292–
3303. https://doi.org/10.1002/jsfa.6175
Rao NR, Kiran SS, Prasanthi NL (2010) Pharmaceutical impurities: an overview. Indian J Pharm
Educ Res 44(3):301–310
Remaud GS, Martin YL, Martin GG, Martin GJ (1997) Detection of sophisticated adulterations
of natural vanilla flavors and extracts: application of the SNIF-NMR method to vanillin and
p-hydroxybenzaldehyde. J Agric Food Chem 45:859–866
Richmond HH (1950) Preparation of cinnamaldehyde. United States Patent 2,529,186
Roy J (2002) Pharmaceutical impurities—A mini-review. AAPS PharmSciTech 3(2):1–6
Royals EE, Horne SE (1951) Conversion of d-limonene to l-carvone. J Am Chem Soc
73(12):5856–5857
Sadgrove N, Jones G (2015) A contemporary introduction to essential oils: chemistry, bioactiv-
ity and prospects for Australian agriculture. Agriculture 5:48–102. https://doi.org/10.3390/
agriculture5010048
Schipilliti L, Dugo P, Bonaccorsi I, Mondello L (2012) Authenticity control on lemon essential
oils employing gas chromatography – combustion-isotope ratio mass spectrometry (GC –
C-IRMS). Food Chem 131:1523–1530. https://doi.org/10.1016/j.foodchem.2011.09.119
Schmidt BM (2016) Regulatory and quality issues with the essential oils supply chain. In: Jeliazkov
VD, Cantrell CL (eds) Medicinal and aromatic crops: production, phytochemistry, and uti-
lization. American Chemical Society, Washington, DC, pp 27–48. https://doi.org/10.1021/
bk-2016-1218.ch003
Schnaubelt K (1999) Medical aromatherapy: healing with essential oils. Frog Books, Berkeley
Sell CS (2003) A fragrant introduction to terpenoid chemistry. Royal Society of Chemistry,
Cambridge
Shu CK, Lawrence BM (1997) Reasons for the variation in composition of some commercial
essential oils. In: Risch SJ, Ho CT (eds) Spices. American Chemical Society, Washington, DC,
pp 138–159. https://doi.org/10.1021/bk-1997-0660.ch012
Singh AG, Hamal JP (2013) Traditional phytotherapy of some medicinal plants used by Tharu
and Magar communities of western Nepal against dermatological disorders. Sci World
11(11):81–89
Stipanovic RD, Puckhaber LS, Bell AA, Percival AE, Jacobs J (2005) Occurrence of (+)- and
(−)-gossypol in wild species of cotton and in Gossypium hirsutum var. marie-galante (Watt).
J Agric Food Chem 53:6266–6271
Stolle A, Ondruschka B, Hopf H (2009) Thermal rearrangements of monoterpenes and monoterpe-
noids. Helv Chim Acta 92:1673–1719
Suga T, Lee YS, Hirata T (1983) The hydroxylation of β-terpineol and its acetate with the cultured
cells of Nicotiana tabacum. Bull Chem Soc Jpn 56(3):784–787
Sugawara Y, Hara C, Aoki T, Sugimoto N, Masujima T (2000) Odour distinctiveness between
enantiomers of linalol: difference in perception and responses elicited by sensory test and fore-
head surface potential wave measurement. Chem Senses 25:77–84
Surburg H, Panten J (2006) Common fragrance and flavor materials, 5th edn. Wiley-VCH,
Weinheim
Tang F, Zhang Q, Nie Z, Chen B, Yao S (2009) Sample preparation for analyzing traditional Chinese
medicines. Trends Anal Chem 28(11):1253–1262. https://doi.org/10.1016/j.trac.2009.09.004
Tisserand R, Young R (2014) Essential oil safety, 2nd edn. Churchill Livingstone Elsevier,
Edinburgh
9 Adulteration Analysis in Essential Oils 273
Tranchida PQ, Bonaccorsi I, Dugo P, Mondello L, Dugo G (2012) Analysis of Citrus essential
oils: state of the art and future perspectives. A review. Flavour Fragr J 27:98–123. https://doi.
org/10.1002/ffj.2089
Traynor S (2001) The musk dilemma. Perfumer Flavorist 26(5):28–39
Turek C, Stintzing FC (2013) Stability of essential oils: a review. Compr Rev Food Sci Food Saf
12:40–53. https://doi.org/10.1111/1541-4337.12006
Wimmer P, Buysch HJ, Puppe L (1991) Process for the preparation of thymol. United States Patent
5,030,770
World Health Organization, WHO (n.d.) Traditional and complementary medicine. http://who.int/
medicines/areas/traditional/en/. Accessed 25 Sep 2017
Chapter 10
Essential Oils from Pines: Chemistry
and Applications
10.1 Introduction
Essential oils (EOs), also referred as ethereal or volatile oils, have been used for
extensive applications in pharmaceutical, medical, and perfume industries. They are
aromatic oily liquids and obtained from different parts of plant, i.e., leaves, seeds,
fruits, buds, flowers, wood, herbs, barks, and roots (Burt 2004). Till now, around
3000 EOs have been reported by scientific community, of which about 300 are of
commercial importance (van de Braak and Leijten 1994). EOs are aromatic oily
liquids and therefore have low solubility in water, however are soluble in alcohols,
organic solvents, fats, and other hydrophobic substances. They are generally liquid
at room temperature (Thormar 2011). Essential oils contribute only a small propor-
tion of the wet weight of plant material, which is usually 1% or less (Pengelly
2004). Essential oils’ content can vary in quality, quantity, and composition accord-
ing to geographical conditions, soil composition, specific parts of plant, and plant
age (Masotti et al. 2003; Angioni et al. 2006). Further, the chemical compositions of
essential oils not only vary in terms of the number of molecules but also in the ste-
reochemical types of molecules extracted, or oil extraction method. Hence, the
essential oil extraction method is chosen accordingly for different applications.
Steam distillation is among the most conventional methods used to extract EOs for
commercial production.
Essential oils are generally described as secondary plant metabolites, which are
synthesized by the plant however not necessarily be essential for plant growth and
development (Croteau et al. 2000). Further, all plants do not universally synthesize
essential oils, though primary metabolites are synthesized by all plants and take part
Caryophyllene C15H24 0.2(β) 6.51 1.0(β) 0.2(β) 3.8(β) 2.2(β) 5.01(β) 21.9 2.87(β) –
Terpinene- C10H18O 0.3 0.22 – – – 0.1 0.26 0.2 0.01 –
4-ol
γ-Muurolene – 0.2 2.62 – – 1.4 0.1 – – – –
Phellandrene C10H16 – 0.25 – – 0.4(α) – – 0.7(β) – 0.46(β)
α-Terpinene C10H16 0.1 0.59 – – – – – – – –
Thujene C10H16 – 1.07(β) – – – – – – – –
γ -Terpinene C10H16 0.2 0.30 – 1.8 – – – 0.2 – –
p-Cymene C10H14 0.1 0.42 – 0.6 0.2 0.1 0.37 1.9 0.47 –
Germacrene D C15H24 0.3 – 0.9 1.1 12.2 4.9 0.38 – 0.01 –
Spathulenol – – – – – – – – – – –
277
(continued)
278
Table 10.1 (continued)
P. P. P. henryi P. P. peuce
densiflora P. pinea P. halepensis tabulaeformis (Xie P. monticola massoniana P. wallichiana (Hajdari
Molecular (Hong (Macchioni (Macchioni (Xie et al. et al. (Dambolena (Xie et al. (Dambolena et al.
Component formula et al. 2004) et al. 2002) et al. 2002) 2015) 2015) et al. 2016) 2015) et al. 2016) 2016)
β-Pinene C10H16 9.82 1.1 0.7 2.29 0.35 22.8 2.99 34.0 10.9
Camphene C10H16 3.86 0.1 0.6 0.38 0.34 3.5 0.63 1.0 5.8
α-Pinene C10H16 14.44 3.9 61.8 11.08 9.68 21 8.16 14.8 29.0
Sabinene C10H16 0.35 – – 0.15 0.18 – – – 0.06
3-Carene C10H16 – – – 0.31 0.13 4.2 0.13 – 0.4
Myrcene C10H16 12.19 2.5 20.1 – 1.04 4.2 – 1.3 0.9
α-Terpineol C10H18O 0.50 0.8 0.2 3.43 1.32 1.3 3.34 0.3 3.4
Terpinolene C10H16 2.87 – – 0.24 (α) 0.59 (α) 5.1 0.17(α) – 0.13
Limonene C10H16 4.34 75.3 0.8 0.41 0.09 14.0 – 17.8 –
Bornyl acetate – 5.67 – – 4.13 2.96 – 3.83 – 7.4
Caryophyllene C15H24 3.26 3.7 8.5 22.36 (β) 18.26(β) 0.5(β) 18.48(β) 1.8(β) 0.1(β)
Terpinene- C10H18O 0.30 – – 0.15 – – 0.2 0.1
4-ol
γ-Muurolene – 0.64 – – 1.06 – – – – –
Phellandrene C10H16 0.45 – – 0.12(β) 0.37 1.8(α) 0.66(β) 0.3(α) 45.1(α)
α-Terpinene C10H16 0.33 – – – 0.12 0.3 0.29 0.6 0.06
Thujene C10H16 19.33 – – 0.13 (α) 1.72(α) 0.4(α) 2.02(α) 0.1(α) –
γ-Terpinene C10H16 0.33 0.1 0.1 0.48 0.13 0.6 0.52 – 0.3
p-Cymene C10H14 0.49 0.1 0.1 – – – – 0.1 –
Germacrene D C15H24 – 0.2 0.6 7.43 2.71 – 9.78 – 16.6
Spathulenol – – – – 0.23 0.65 – – – 0.1
All the concentrations are shown on % basis. In literature, variation in constituent’s concentrations may exist due to change in geographical and climate con-
ditions
G. K. Silori et al.
10 Essential Oils from Pines: Chemistry and Applications 279
10.2 P
ine Essential Oils: Major Constituent
and Corresponding Yields
Pine essential oils contain varieties of organic constituents. Though the number of
constituents is more than 50 (Tumen et al. 2010), 20 major constituents have been
classified by different sources to investigate their respective yields in different pine
species across the world. The major components in pine EOs are β-pinene, camphene,
α-pinene, sabinene, 3-carene, myrcene, α-terpineol, terpinolene, limonene, bornyl
acetate, caryophyllene, terpinene-4-ol, γ-muurolene, phellandrene, α-terpinene, thu-
jene, γ-terpinene, p-cymene, germacrene D, and spathulenol. Figure 10.1 shows
chemical structures of 20 major contributors in pines’ essential oils. Variations in the
yield of selected constituent as per their origin and species can be seen from Table 10.1.
It can be stated that five constituents, namely, α-pinene, β-pinene, camphene, 3-carene,
and myrcene, are the major contributors in essential oil formation in pines.
According to Royal Botanic Gardens and Kew and Missouri Botanical Garden,
there are around 126 recognized species, with 35 unresolved species and many
more synonyms (http://www.missouribotanicalgarden.org/ 2018; https://www.kew.
org/ 2018). As mentioned earlier, pine has a very large family, and they are widely
spread in most parts of the globe. Forty major pine species and their availability
across the globe has been mentioned in Table 10.2, of which 25 species belongs to
Pinus subgenus while 15 are from Strobus. Figure 10.2 shows availability percent-
age of investigated species in different continents. It is evident from Fig. 10.2 that
Asian, European, and North American continents together comprise around 40% of
major pine species found across the globe.
Fig. 10.1 Major constituents and their chemical structure in pine’s essential oil
10 Essential Oils from Pines: Chemistry and Applications 281
Table 10.2 Pine species and their availability across the globe
Section Species Common name Country/region
Pinus P. brutia Eastern Mediterranean or Turkey, Crimea, Iran
Calabrian pine
P. halepensis Aleppo pine Morocco, Algeria, Spain
P. heldreichii Bosnian pine Southern Italy, Croatia
P. pinaster Maritime or cluster pine Portugal, Northern Spain
P. pinea Mediterranean stone or Israel, South Africa, New South
umbrella pine Wales
P. roxburghii Chir pine India, Nepal, Tibet, Pakistan
P. densiflora Japanese red pine Japan, Northeastern China,
Southeast Russia
P. massoniana Masson pine Taiwan, Hong Kong, Northern
Vietnam
P. mugo Dwarf mountain pine Southwest Europe, Austria,
Switzerland
P. mugo var. Dwarf mountain pine North and Central Italy
prostrata
P. nigra subsp. Corsican pine France and Italy
laricio
P. sylvestris subsp. Scot pine Western Europe to Eastern
scotica Siberia
P. thunbergii Japanese black pine Japan, South Korea
P. attenuata Knobcone pine Southern Oregon
P. patula Mexican weeping pine Mexico, Bolivia, Kenya
P. coulteri Coulter or bigcone pine Southern California, Mexico
P. jeffreyi Jeffrey pine California, Oregon
P. torreyana Torrey pine San Diego, Santa Rosa Island
P. teocote Mexican small-cone or Aztec Mexico
pine
P. sylvestris Scots pine Eurasia, Siberia
P. tabuliformis Chinese red pine China, North Korea
P. elliottii Slash pine Southeastern United States
P. radiata Monterey pine Central Coast of California
P. ponderosa Ponderosa or western yellow Western United States and
pine Canada
P. banksiana Jack pine Canada, Minnesota
(continued)
282 G. K. Silori et al.
Table 10.2 (continued)
Section Species Common name Country/region
Strobus P. aristata Colorado bristlecone pine United States
P. cembroides Mexican pinyon Texas, Mexico
P. monophylla Single leaf pinyon Arizona, Northwest Mexico
P. bungeana Lacebark pine Northeastern and Central China
P. gerardiana Chilgoza or Gerard’s pine Afghanistan, Pakistan, and
northwest India
P. armandii Chinese white or Armand’s China, Taiwan
pine
P. cembra Swiss stone or Arolla pine Poland, Austria, Germany
P. flexilis Limber or Rocky Mountain Western United States, Canada
white pine
P. koraiensis Korean stone pine Korea, Northeastern China,
Mongolia
P. monticola Western white pine United States, Canada
P. parviflora Japanese white pine Korea and Japan
P. pumila Dwarf stone pine Eastern Siberia, Northern Japan,
and Korea
P. peuce Macedonian or Balkan (white) Bulgaria, Albania
pine
P. strobus Eastern white pine Eastern North America
P. wallichiana Himalayan blue pine Himalaya, Bhutan
Farjon 2005; Ioannou et al. 2014; Gernandt et al. 2005; Richardson and Rundel 1998
Fig. 10.2 Percentage availability of considered pine species across the globe
10 Essential Oils from Pines: Chemistry and Applications 283
Fig. 10.3 Progress in essential oil’s extraction techniques in last four decades
The vapor produced by the steam generator passes through the ground plant mate-
rial, stored in apparatus. The vapors generated are then condensed using a con-
denser, and the condensate is collected in a flask. The collected oil is dried with
anhydrous Na2SO4 and stored at particular temperature until used. Extraction needs
to be performed at least three times (Sahraoui et al. 2008). Figure 10.4 shows a typi-
cal setup of steam distillation extraction process.
10.4.2 Maceration
The technique involves soaking of raw plant material, which is generally grinded/
powdered form, in a close container with a solvent for a period of over 72 h. The
container is frequent agitated, the process is held at room temperature. Conduction
and convections are the two mediums of heat transfer in this method. The solvent is
chosen according to desired component, for example, methanol is best solvent for
extraction of secondary metabolites (Handa et al. 2008).
284 G. K. Silori et al.
Supercritical fluid (SF), which is also known as dense-gas, is a special class of sol-
vents containing the solvating property of liquids but behaves like a gas, simultane-
ously (Orav et al. 1998). The plant material is extracted with supercritical fluid
which is generally carbon dioxide (Schaneberg and Khan 2002). For supercritical
CO2, extraction temperature and pressure are maintained above 304 K and 74 bar,
respectively. The properties of supercritical fluid can be altered through change in
temperature and pressure. However, high pressure requirement in this process
makes it costly when compared to conventional extraction processes.
10 Essential Oils from Pines: Chemistry and Applications 285
It is also referred as sonication extraction and involves the use of ultrasound irradia-
tion, which generally ranges from 20 kHz to 2000 kHz. The extraction is performed
in an ultrasound cleaning bath with the help of indirect sonication. The ground
material is charged with the batch of organic solvent (polar or nonpolar as per sam-
ple requirement). The sonication is held for a particular time, which is generally
10.4.9 M
icrowave Hydro-Diffusion and Gravity (MHG)
Extraction
MHG is among newly developed green techniques for extraction of EOs. It is based
on two phenomena, i.e., hydro-diffusion and gravity. It is alike SFME till release of
extract (water and EOs). Further, hydro-diffusion phenomenon allows the extract to
diffuse outside the plant material. The extract is collected and separated in a vessel
10 Essential Oils from Pines: Chemistry and Applications 287
based on their specific gravity. Because of its least energy requirements, MHG has
established its superiority over existing MAE, SFME, or modified hydro-distillation
techniques. In a study, MHG was compared with a conventional technique, hydro-
distillation (HD), for the extraction of essential oil from two aromatic herbs. The
essential oils extracted through MHG were quantitatively (yield) and qualitatively
(aromatic profile) similar to those obtained by conventional hydro-distillation while
reducing extraction time by six times (Vian et al. 2008). When it comes to industrial
implications, MHG technique is highly recommended having least amount of
greenhouse gases. A schematic of MHG extraction process is shown in Fig. 10.6.
Being a key precursor for flavonoids and lignin, shikimic acid holds position as a
key synthetic intermediate for plants (Bu’Lock 1965; Mann et al. 1994). While
flavonoids act as antioxidants and UV protector, lignin is responsible for woody
tissue formation in plants. Phosphoenolpyruvate and erythrose-4 phosphate are the
two major synthesizers of shikimic acid in plants as shown in Fig. 10.7. Benzoic
acid and its derivate, widespread for their use as ester, are formed by the aromatiza-
tion of shikimic acid provided no further addition of three carbon atoms from phos-
phoenolpyruvate (Mann et al. 1994).
As far as EOs are concerned, terpenoid is among the most important pathways exist-
ing in pine species (Singh and Sharma 2015; Eggersdorfer 2012). When first formed,
terpene structure contains a multiple of five carbon atoms. Terpenoid with 10 carbon
atoms per molecule are referred as monoterpenoids. Further, terpenoids with 5, 15,
From various literature resources, it has been found that pines contain several medic-
inal properties, which can be utilized for the interest of mankind. Various efforts have
been made over last few decades to identify antimicrobial and antiviral properties of
pines. Moreira et al. (2005) examined the antimicrobial activities of essential oils
against four strains of E. coli. On the basis of findings, it was suggested that the EO
can be used as food antimicrobial preservatives (Moreira et al. 2005). Bhalla et al.
(2013) successfully reviewed the possibility of essential oils’ constituents for the
treatment of cancerous disease. It was reported that EOs have potential to enhance
activity of white blood cells, which are responsible for removing foreign materials
and microbes from the body (Bhalla et al. 2013).
It has been reported that the pine needles comprise about 1.5–2.5% shikimic acid,
although a little change with season and age has also been observed. The studies car-
ried out in available literature confirm that few pine species contain a good enough
amount of a key precursor which is used in the production of Tamiflu®, an antiviral
drug used in the treatment of H5N1 flu (Xie et al. 2012; Sui 2008). Chen et al. (2014)
achieved about a 6% yield of shikimic acid from Masson pine needles, which is
possibly the highest extracted yield from any pine species till now (Chen et al. 2014).
Since pine needles are inexpensive and readily available in North Asia, North
America, and Europe, there is a strong possibility to utilize them as a drug manufac-
turer against less available star anise species.
Another major ingredient in pine EOs are terpenoids, which have antimicrobial,
antifungal, antiparasitic, antiviral, anti-allergenic, antispasmodic, antihyperglyce-
mic, anti-inflammatory, and immunomodulatory properties and have been found
useful in the prevention and therapy of several diseases, including cancer (Theis and
Lerdau 2003; Medeiros Leite et al. 2007). Terpenoids have also been found effective
as natural insecticides and can be used in storing agriculture products. Various stud-
ies have suggested to pay substantial attention to introduce terpenes into modern
therapies (Paduch et al. 2007). Wound-healing mechanism can be enhanced by
incorporating EOs in polymer matrix.
Resin is a viscous liquid generally obtained from the pine tree bark. Rosin,
which is obtained from the distillation of resin, has many applications. Three
esters of rosin, viz., glycerol, sorbitol, and mannitol, are proposed to be used in
chewing gums and sweeteners as they contain antidiabetic properties (Fulzele
et al. 2004). Rosins have been utilized for manufacturing microcapsules and
nanoparticles, and their properties have been examined in vitro and ex vivo studies
(Lee et al. 2005).
10 Essential Oils from Pines: Chemistry and Applications 291
While pines contain several medicinal properties, they have industrial significance
too. Terpenes, one of the major constituents of pine’s essential oil, have use in the
preparation of perfumes and insect repellants. Turpentine oil is used in the prepara-
tion of cosmetics, further the characteristic fragrances of terpenes make them useful
in aromatherapy too. Terpenes like limonene and pinene are used as air fresheners
(Kandi et al. 2015).
Rosin is useful in the manufacturing of adhesives, printing ink, soldering fluxes,
varnishes, and sealing waxes. It has also been used as a glazing agent in many con-
sumables including medicines and chewing gums. Other uses of rosin include plas-
ters and ointments where it forms a key ingredient. Rosin has a good electric
isolation and thus has suitability to be used in electric cables. Rosin has been mixed
with pitch to be used in optical component making (Silori et al. 2013).
Besides essential oils from pines, pine needles have also been used to produce
electricity at large scale through gasification process. A 10 KV plant setup has
been installed to produce electricity from dry pine needles in Uttarakhand state of
India (Mishra and Vlosky 2015; http://avani-kumaon.org/ n.d.). As pine species
are sufficiently available across the globe, the work has fair chance to meet huge
electricity demand of beneficiaries. Table 10.3 shows applications of the major
EOs’ constituents.
10.7 Summary
As evident from the present study and available literature, it can be clearly stated that
pines have contributed extensively in essential oils. Essential oils from pines and
pine needles have been used for diverse range of applications varying from pharma-
ceuticals, medical, etc. to energy production for domestic (in rural areas) as well as
industrial applications. Essential oils from pine contain more than 50 components in
which five constituents, namely, α-pinene, β-pinene, camphene, 3-carene, and myr-
cene, are major. Further, with the advent in process intensification in process industry
new technologies have been developed for the extraction of EOs from pine. A few of
them, such as ultra-sonication irradiation and microwave-assisted techniques, are six
to ten times faster than the conventional techniques. However, these techniques are
still mainly used in the laboratories and not much attention has been made at the
industrial level. Further, two major metabolite pathways for shikimate and terpenoids
were discussed in brief as they have high medicinal values.
292 G. K. Silori et al.
Table 10.3 Major constituents of pines’ essential oil and their applications
Pines’ EO
constituents Uses Ref.
β-Pinene Cleaning solvent, antimicrobial Kelkar et al. (2006); Rivas da Silva
properties against bacterial cells et al. (2012)
Camphene Food additives, plasticizer for resins Verschueren (2001); Sell (2006)
and lacquers, feedstock for
preparation of fragrance components
α-Pinene Cosmetics and odor agents, O’Neil (2006); Lewis Sr. (2007)
insecticides, camphene, esters,
lubricating oil solvents, and
plasticizers
Sabinene Exhibits anti-inflammatory, Valente et al. (2013)
antimicrobial, and antifungal
properties
3-Carene Feedstock for perfumes, cosmetics, Ocete et al. (1989); Cavaleiro et al.
flavors, and terpene resins, serves as (2006)
an antihistamine by reducing excess
menstrual flow or perspiration,
delta-3-carene is used as a central
nervous system depressant
Myrcene Production of 7-hydroxygeranyl- Eggersdorfer (2012); Lewis Sr. (2007)
neryl dialkylamine
Feedstock for geraniol, nerol,
linalool, and isophytol. Preparation
of perfume chemicals and flavoring
α-Terpineol Solvent for resins and cellulose Lewis Sr. (2007); Tobin et al. (1976)
esters and ethers
Perfumes, soaps, disinfectant,
antioxidant, flavoring agent
Terpinolene Solvent for resins, manufacturing of Lewis Sr. (2007); Eggersdorfer (2012)
synthetic resins and synthetic flavors,
improvement the odor of industrial
and household products
Limonene Starting material for the synthesis of Sell (2006); Lewis Sr. (2007);
(R)-(−)-carvone, gallstone Shepard (1986); Bingham et al.
solubilizer, used in many food, soap, (2001)
and perfume products for its
lemon-like flavor. Has antimicrobial,
antiviral, antifungal, antilarval, insect
attractant, and repellent properties
Bornyl acetate Used in perfumes and for flavoring. de Cássia da Silveira e Sá et al.
Bornyl acetate exhibit anti- (2013)
inflammatory property and it is used
as an analgesic
Caryophyllene Anti-inflammatory and analgesic, Bahi et al. (2014); Klauke et al.
antianxiety, and antidepressant (2014)
Terpinene-4-ol Pepper oils and in perfumery, Fahlbusch et al. (2003)
medication, and therapeutics
Phellandrene Flavoring and perfumery Hawley (1977)
(continued)
10 Essential Oils from Pines: Chemistry and Applications 293
Table 10.3 (continued)
Pines’ EO
constituents Uses Ref.
α -Terpinene Maintain the oxidative stability of Rudback et al. (2012)
food, cosmetics, and medicaments,
for odor purposes in industrial fluids
Thujene Antimicrobial activity Sadhasivam et al. (2016)
γ -Terpinene In the production of pharmaceutical Soukoulis and Hirsch (2004);
drugs and perfumes. Cytotoxic, Sharifi-Rad et al. (2015)
antimicrobial, and anti-inflammatory
properties
p-Cymene Manufacturing of p-cresol and Sell (2006); Lewis Sr. (2007);
carvacrol, used as a solvent and heat Eggersdorfer (2012); Fahlbusch et al.
transfer fluid, feedstock for the (2003)
polycyclic musk (fixolide or
tonalide), perfumery, solvent for dyes
and varnishes
Germacrene D Antibacterial property, cytotoxicity Salvador et al. (2011); Olajuyigbe and
Ashafa (2014)
Spathulenol Anesthetic in nature and used to https://www.ebi.ac.uk/chebi/searchId.
manufacture a drug which is used to do?chebiId=CHEBI:132824 (2018)
cause dilation of the blood vessels
References
Angioni A, Barra A, Coroneo V, Dessi S, Cabras P (2006) Chemical composition, seasonal vari-
ability, and antifungal activity of Lavandula stoechas L. ssp. stoechas essential oils from stem/
leaves and Flowers. J Agric Food Chem 54:4364–4370
Bahi A, Al Mansouri S, Al Memari E, Al Ameri M, Nurulain SM, Ojha S (2014) beta-
Caryophyllene, a CB2 receptor agonist produces multiple behavioral changes relevant to
anxiety and depression in mice. Physiol Behav 135:119–124
Bakkali F, Averbeck S, Averbeck D, Idaomar M (2008) Biological effects of essential oils – a
review. Food Chem Toxicol 46:446–475
K. Başer, G. Buchbauer, Handbook of essential oils: science, technology, and applications, 2010,
CRC Press (Taylor & Francis), Boca Ration
Behr A, Johnen L (2009) Myrcene as a natural base chemical in sustainable chemistry: a critical
review. ChemSusChem 2:1072–1095
Bhalla Y, Gupta VK, Jaitak V (2013) Anticancer activity of essential oils: a review. J Sci Food
Agric 93:3643–3653
Bigley WS, Plapp FW, Hanna RL, Harding JA (1981) Effect of toxaphene, camphene, and cedar
oil on methyl parathion residues on cotton. Bull Environ Contam Toxicol 27:90–94
Bingham E, Cohrssen B, Powell CH (2001) Patty’s Toxicology, 5th edn. Wiley, New York
Bu’Lock JD (1965) The biosynthesis of natural products; an introduction to secondary metabo-
lism. McGraw-Hill, London/New York
Burt S (2004) Essential oils: their antibacterial properties and potential applications in foods—a
review. Int J Food Microbiol 94:223–253
de Cássia da Silveira e Sá R, Andrade LN, de Sousa DP (2013) A review on anti-inflammatory
activity of monoterpenes. Molecules 18:1227–1254
294 G. K. Silori et al.
Cavaleiro C, Pinto E, Goncalves MJ, Salgueiro L (2006) Antifungal activity of Juniperus essential
oils against dermatophyte, Aspergillus and Candida strains. J Appl Microbiol 100:1333–1338
Chen X, Li Yuan LW, Wang M, Lei Y (2014) Content analysis of Shikimic acid in the Masson pine
needles and antiplatelet-aggregating activity. Int J Agric Sci Technol 2:110–114
Croteau R, Kutchan TM, Lewis NG (2000) Natural products (secondary metabolites). In:
Biochemistry and molecular biology of plants. American Society of Plant Physiologists, Rock
Ville, pp 1250–1318
Dambolena JS, Gallucci MN, Luna A, Gonzalez SB, Guerra PE, Zunino MP (2016) Composition,
antifungal and antifumonisin activity of Pinus wallichiana, Pinus monticola and Pinus strobus
essential oils from Patagonia Argentina. J Essent Oil-Bearing Plants 19:1769–1775
van de Braak SAAJ, Leijten GCJJ (1994) A.I. (’s-H.T.O. for Education, Research, Essential oils
and oleoresins: a survey in the Netherlands and other major markets in the European Union,
CBI, Centre for the Promotion of Imports from Developing Countries
Eggersdorfer M (2012) Terpenes. In: Ullmann’s encyclopedia of industrial chemistry, 7th edn.
Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. https://doi.org/10.1002/14356007.
a26_205
Fahlbusch K-G, Hammerschmidt F-J, Panten J, Pickenhagen W, Schatkowski D, Bauer K,
Garbe D, Surburg H (2003) Flavors and fragrances. Wiley Online Library, https://doi.
org/10.1002/14356007.a11_141
Farjon A (2005) Pines: drawings and descriptions of the genus Pinus. Brill Publishers, Leiden
Filly A, Fernandez X, Minuti M, Visinoni F, Cravotto G, Chemat F (2014) Solvent-free microwave
extraction of essential oil from aromatic herbs: From laboratory to pilot and industrial scale.
Food Chem 150:193–198
Fulzele SV, Satturwar PM, Kasliwal RH, Dorle AK (2004) Preparation and evaluation of micro-
capsules using polymerized rosin as a novel wall forming material. J Microencapsul 21:83–89
Gernandt DS, Geada López G, Ortiz García S, Liston A (2005) Phylogeny and classification of
Pinus. Taxon 54:29–42
Hajdari A, Mustafa B, Nebija D, Selimi H, Veselaj Z, Breznica P, Quave CL, Novak J (2016)
Essential oil composition of Pinus peuce Griseb. needles and twigs from two national parks of
Kosovo. Sci World J 2016
Hajhashemi V, Ghannadi A, Sharif B (2003) Anti-inflammatory and analgesic properties of the leaf
extracts and essential oil of Lavandula angustifolia mill. J Ethnopharmacol 89:67–71
Handa SS, Khanuja SPS, Longo G, Rakesh DD (2008) Extraction technologies for medicinal and
aromatic plants. ICS UNIDO, Trieste, Italy
Hassan A, Amjid I (2009) Gas chromatography-mass spectrometric studies of essential oil of Pinus
roxburghaii stems and their antibacterial and antifungal activities. J Med Plants Res 3:670–673
Hawley GG (1977) Condensed chemical dictionary, 9th edn. Van Nostrand Reinhold Inc.,
New York
Hong E-J, Na K-J, Choi I-G, Choi K-C, Jeung E-B (2004) Antibacterial and antifungal effects of
essential oils from coniferous trees. Biol Pharm Bull 27:863–866
http://avani-kumaon.org/, (n.d.). Accessed on 06-08-2018
http://www.missouribotanicalgarden.org/. Accessed on 06-08-2018
https://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI:132824. Accessed on 06-08-2018
https://www.kew.org/. Accessed on 06-08-2018
Ioannou E, Koutsaviti A, Tzakou O, Roussis V (2014) The genus Pinus: a comparative study on the
needle essential oil composition of 46 pine species. Phytochem Rev 13:741–768
Jensen WB (2007) The origin of the Soxhlet extractor. J Chem Educ 84:1913
Kandi S, Godishala V, Rao P, Ramana KV (2015) Biomedical significance of terpenes: an insight.
Biomed Biotechnol 3:8–10
Kaushik D, Kumar A, Kaushik P, Rana AC (2012) Analgesic and anti-inflammatory activity of
pinus roxburghii sarg. Adv Pharmacol Sci 2012
Keeley JE (2012) Ecology and evolution of pine life histories. Ann For Sci 69:445–453
Kelkar VM, Geils BW, Becker DR, Overby ST, Neary DG (2006) How to recover more value from
small pine trees: essential oils and resins. Biomass Bioenergy 30:316–320
10 Essential Oils from Pines: Chemistry and Applications 295
Kimbaris AC, Siatis NG, Daferera DJ, Tarantilis PA, Pappas CS, Polissiou MG (2006) Comparison
of distillation and ultrasound-assisted extraction methods for the isolation of sensitive aroma
compounds from garlic (Allium sativum). Ultrason Sonochem 13:54–60
Klauke A-L, Racz I, Pradier B, Markert A, Zimmer AM, Gertsch J, Zimmer A (2014) The cannabi-
noid CB(2) receptor-selective phytocannabinoid beta-caryophyllene exerts analgesic effects in
mouse models of inflammatory and neuropathic pain. Eur Neuropsychopharmacol 24:608–620
Krauze-Baranowska M, Mardarowicz M, Wiwart M, Pobłocka L, Dynowska M (2002) Antifungal
activity of the essential oils from some species of the genus Pinus. Zeitschrift Für Naturforsch
C 57:478–482
Lee CM, Lim S, Kim GY, Kim DW, Joon HR, Lee KY (2005) Rosin nanoparticles as a drug deliv-
ery carrier for the controlled release of hydrocortisone. Biotechnol Lett 27:1487–1490
Lewis RJ Sr (2007) Hawley’s condensed chemical dictionary, 15th edn. Wiley Online Library,
https://doi.org/10.1002/9780470114735
Lucchesi ME, Chemat F, Smadja J (2004) Solvent-free microwave extraction of essential oil
from aromatic herbs: Comparison with conventional hydro-distillation. J Chromatogr A
1043:323–327
Macchioni F, Cioni PL, Flamini G, Morelli I, Perrucci S, Franceschi A, Macchioni G, Ceccarini L
(2002) Acaricidal activity of pine essential oils and their main components against Tyrophagus
putrescentiae, a stored food mite. J Agric Food Chem 50:4586–4588
MacTavish WC (1934) A textbook of organic chemistry. Third edition revised (Chamberlain,
Joseph Scudder). J Chem Educ 11:633
Mann J, Davidson RS, Hobbs JB, Banthorpe DV, Harborne JB (1994) Natural product-their chem-
istry and biological significance. Longman, Harlow
Masotti V, Juteau F, Bessière JM, Viano J (2003) Seasonal and phenological variations of the
essential oil from the narrow endemic species Artemisia molinieri and its biological activities.
J Agric Food Chem 51:7115–7121
Medeiros Leite A, De Oliveira Lima E, Leite De Souza E, De M, Diniz MF, Trajano VN, Almeida
De Medeiros I (2007) Inhibitory effect of β-pinene, α-pinene and eugenol on the growth of
potential infectious endocarditis causing Gram-positive bacteria. Rev Bras Ciências Farm
Brazilian J Pharm Sci 43:121–126
A. Mishra, R. Vlosky, A Case Study of Electricity Generation from Pine Needles in Rural
Uttarakhand, India, Louisiana State University Agriculture Center, Baton Rouge (LA) (2015)
http://www.woodema.org/conferences/2015_Dubrovnik_presentations/F06_Mishra-Vlosky.
pdf
Moreira MR, Ponce AG, Del Valle CE, Roura SI (2005) Inhibitory parameters of essential oils to
reduce a foodborne pathogen. LWT – Food Sci Technol 38:565–570
O’Neil MJ (2006) The Merck Index – an encyclopedia of chemicals, drugs, and biologicals. Royal
Society of Chemistry (UK)
Ocete MA, Risco S, Zarzuelo A, Jimenez J (1989) Pharmacological activity of the essential
oil of Bupleurum gibraltaricum: anti-inflammatory activity and effects on isolated rat uteri.
J Ethnopharmacol 25:305–313
Olajuyigbe O, Ashafa A (2014) Chemical Composition and Antibacterial Activity of Essential Oil
of Cosmos bipinnatus Cav. Leaves from South Africa. Iran J Pharm Res 13:1417–1423
Orav A, Kailas T, Koel M (1998) Simultaneous distillation, extraction and supercritical fluid
extraction for isolating volatiles and other materials from conifer needles. J Essent Oil Res
10:387–393
Paduch R, Kandefer-Szerszeń M, Trytek M, Fiedurek J (2007) Terpenes: substances useful in
human healthcare. Arch Immunol Ther Exp 55:315–327
Pengelly A (2004) The constituents of medicinal plants: an introduction to the chemistry and thera-
peutics of herbal medicines, 2nd edn. Allen & Unwin Academic, Crows Nest, New South Wales
Perry NSL, Bollen C, Perry EK, Ballard C (2003) Salvia for dementia therapy: review of pharma-
cological activity and pilot tolerability clinical trial. Pharmacol Biochem Behav 75:651–659
Richardson DM, Rundel PW (1998), Ecology and biogeography of Pinus: an introduction. In:
Richardson, D.M. (Ed.), Ecology and Biogeography of Pinus. Cambridge University Press,
Cambridge
296 G. K. Silori et al.
Richardson DM, Ryan MG (1998) The complete pine. In: Ecology and biogeography of Pinus.
Cambridge University Press, Cambridge
Rivas da Silva AC, Lopes PM, Barros de Azevedo MM, Costa DC, Alviano CS, Alviano DS (2012)
Biological activities of α-pinene and β-pinene enantiomers. Molecules 17:6305–6316
Rudback J, Bergstrom MA, Borje A, Nilsson U, Karlberg A-T (2012) alpha-Terpinene, an anti-
oxidant in tea tree oil, autoxidizes rapidly to skin allergens on air exposure. Chem Res Toxicol
25:713–721
Sadhasivam S, Palanivel S, Ghosh S (2016) Synergistic antimicrobial activity of Boswellia serrata
Roxb. ex Colebr. (Burseraceae) essential oil with various azoles against pathogens associated
with skin, scalp and nail infections. Lett Appl Microbiol 63:495–501
Sahraoui N, Vian MA, Bornard I, Boutekedjiret C, Chemat F (2008) Improved microwave steam
distillation apparatus for isolation of essential oils. Comparison with conventional steam distil-
lation. J Chromatogr A 1210:229–233
Salvador MJ, de Carvalho JE, Wisniewski-Jr A, Kassuya CAL, Santos ÉP, Riva D, Stefanello
MÉA (2011) Chemical composition and cytotoxic activity of the essential oil from the leaves
of Casearia lasiophylla. Rev Bras Farmacogn 21:864–868
Schaneberg BT, Khan IA (2002) Comparison of extraction methods for marker compounds in the
essential oil of lemon grass by GC. J Agric Food Chem 50:1345–1349
Sell CS (2006) Terpenoids. In: Kirk-Othmer encyclopedia of chemical technology. Wiley Online
Library. https://doi.org/10.1002/0471238961.2005181602120504.a01.pub2
Sharifi-Rad J, Sharifi-Rad M, Hoseini-Alfatemi SM, Iriti M, Sharifi-Rad M, Sharifi-Rad M (2015)
Composition, cytotoxic and antimicrobial activities of Satureja intermedia C.A.Mey essential
oil. Int J Mol Sci 16:17812–17825
Shepard TH (1986) Catalog of teratogenic agents, 5th edn. The Johns Hopkins University Press,
Maryland
Silori GK, Pant G, Singh JK, Kumar P (2013) Hay and Tendril classifiable deforestation of pine
and its impacts – a survey of Himalayan Region in India. IOSR J Environ Sci Toxicol Food
Technol 6:06–14
Silva J, Abebe W, Sousa SM, Duarte VG, Machado MIL, Matos FJA (2003) Analgesic and anti-
inflammatory effects of essential oils of Eucalyptus. J Ethnopharmacol 89:277–283
Simonsen JL (1931) The terpenes. Cambridge University Press, Cambridge
Singh B, Sharma RA (2015) Plant terpenes: defense responses, phylogenetic analysis, regulation
and clinical applications. 3 Biotech 5:129–151
Soukoulis S, Hirsch R (2004) The effects of a tea tree oil-containing gel on plaque and chronic
gingivitis. Aust Dent J 49:78–83
Sui R (2008) Separation of Shikimic acid from pine needles. Chem Eng Technol 31:469–473
Sun J (2007) D-limonene: safety and clinical applications. Altern Med Rev 12:259–264
Thakker MR, Parikh JK, Desai MA (2016) Microwave assisted extraction of essential oil from
the leaves of Palmarosa: multi-response optimization and predictive modelling. Ind Crop Prod
86:311–319
Theis N, Lerdau M (2003) The evolution of function in plant secondary metabolites. Int J Plant
Sci 164:S93–S102
Thormar H (ed) (2011) Lipids and essential oils as antimicrobial agents, 1st edn. Wiley, Chichester
Tobin T, Swerczek TW, Blake JW (1976) Pine oil toxicity in the horse: drug detection, residues and
pathological changes. Res Commun Chem Pathol Pharmacol 15:291–301
Tumen I, Hafizoglu H, Kilic A, Dönmez IE, Sivrikaya H, Reunanen M (2010) Yields and con-
stituents of essential oil from cones of Pinaceae spp. Natively grown in Turkey. Molecules
15:5797–5806
Valente J, Zuzarte M, Goncalves MJ, Lopes MC, Cavaleiro C, Salgueiro L, Cruz MT (2013)
Antifungal, antioxidant and anti-inflammatory activities of Oenanthe crocata L. essential oil.
Food Chem Toxicol 62:349–354
Verschueren K (2001) Handbook of environmental data on organic chemicals, 4th edn. Wiley,
New York
10 Essential Oils from Pines: Chemistry and Applications 297
Vian MA, Fernandez X, Visinoni F, Chemat F (2008) Microwave hydrodiffusion and gravity, a new
technique for extraction of essential oils. J Chromatogr A 1190:14–17
Xie JY, Lan JF, Yu L, Wu CM (2012) Study on extraction of Shikimic acid from pine needles of
Pinus Elliottii Engelm by means of microwave pretreatment. Adv Mater Res 455–456:752–759
Xie Q, Liu Z, Li Z (2015) Chemical composition and antioxidant activity of essential oil of six
Pinus taxa native to China. Molecules 20:9380–9392
Zafar I, Fatima A, Khan SJ, Rehman Z, Mehmud S (2010) GC-MS studies of needles essential oil
of Pinus roxburghaii and their antimicrobial activity from Pakistan. Electron J Environ Agric
Food Chem 9:468–473
Zhang S, Jiang J, Luan Q (2016) Genetic and correlation analysis of oleoresin chemical compo-
nents in slash pine. Genet Mol Res 15
Part IV
Strategies and Technologies for Essential
Oil Production
Chapter 11
Biotechnological Approaches to Increase
Essential Oil Yield and Quality
in Aromatic Plants: The Lavandula latifolia
(Spike Lavender) Example. Past
and Recommendations for the Future
11.1 Introduction
Aromatic plants are typically defined by their production of so-called essential oils
(EOs), which are stored in glandular trichomes, located on the surface of both flow-
ers and leaves (Hallahan 2000; Tissier et al. 2017). These EOs comprise a combina-
tion of hydrophobic and volatile compounds of variable chemical nature, and the
name “essential” derives from the word “essence,” due to the aromatic nature of the
oils (Lubbe and Verpoorte 2011). EOs have ecological and physiological functions
in plant interactions with biotic and abiotic factors (e.g., pollinator attraction,
defense, plant-to-plant communication, thermotolerance, and environmental stress
adaptation) and in growth and development (Abbas et al. 2017; Dudareva et al.
2013; Jones et al. 2016; Pichersky and Gershenzon 2002; Vivaldo et al. 2017). On
the other hand, EO constituents are of great economic interest for pharmaceutical,
food, and flavor industries, cosmetics, perfumery, and aromatherapy (Lobstein and
Couic-Marinier 2017; Upson and Andrews 2004; Woronuk et al. 2011).
The Lavandula genus of the Lamiaceae family includes approximately 400 reg-
istered cultivars, 39 plant species, and an important number of hybrids (Upson
2002; Upson and Andrews 2004). This category of plants is naturally found in the
Mediterranean area along with Asia and the Middle East, being typical constituents
Fig. 11.1 (a) Spike lavender in its natural environment; (b) detail on spike lavender flowers; (c–e)
spike lavender leaves observed under a scanning electron microscope showing glandular capitate
(ca) or peltate (pe) trichomes and non-glandular trichomes (n). (Photo from JM Muñoz-Bertomeu).
Bars (a) 20 cm; (b) 1 cm; (c) 50 μm
11 Biotechnological Approaches to Increase Essential Oil Yield and Quality… 303
and the aqueous part, rich in biologically active compounds with applications in
cosmetology (Smigielski et al. 2013).
The constituents of an EO may be classified into two principal groups: (1) hydro-
carbons (monoterpenes and sesquiterpenes) and (2) oxygenated compounds derived
from these hydrocarbons, including alcohols, aldehydes, esters, ketones, phenols,
oxides, etc. (Arimura et al. 2017; Ashour et al. 2010).
The amount, chemical composition, and quality of the EOs depend on many
genotypic and phenotypic factors, such as variety, the botanical organ they are iso-
lated from, the plant’s developmental stage, soil and climatic conditions, season of
harvest, drying methods in the case of dry material, or even extraction techniques
(Aprotosoaie et al. 2017; Dušková et al. 2016; Kara and Baydar 2013).
Lavender EOs consist of a complex mixture of tens to hundreds of mono- and
sesquiterpene alcohols, esters, oxides, and ketones (Daviet and Schalk 2010;
Harborne and Williams 2002; Aprotosoaie et al. 2017). The main constituents of
these EOs are monoterpenes, such as camphor, 1,8-cineole, linalyl acetate, linalool,
β-ocimene, or terpinen-4-ol. Sesquiterpenes (as caryophyllene and nerolidol) and
other terpenoids such as perillyl alcohol are also present in traces. A review of the
composition of the essential oil from 17 Lavandula species has been recently pub-
lished (Aprotosoaie et al. 2017). These authors provide a systematic view of the
chemistry of lavender essential oils mainly considering investigations within the
last 15 years. The review reports the characteristic constituents and chemotypes of
each studied Lavandula species; also, the intra- and interspecific chemical variabil-
ity of the oils is discussed in relation to the geographic area, onto- and morphoge-
netic factors, and extraction methods.
The quality of lavender EOs depends first on the amount of desirable major fla-
vor compounds, especially the monoterpenes linalool and linalyl acetate (syn. lin-
alool acetate) that are characteristic terpenes of lavender scent, but also on the
specific aromatic bouquet given by several minor compounds (Dušková et al. 2016).
Only a few L. angustifolia cultivars produce EOs of high quality, which are pre-
dominantly used in the perfume industry (Despinasse et al. 2017). Other monoter-
penes such as camphor and borneol produce an undesirable odor, diminishing the
quality of the oil (Dušková et al. 2016). Nevertheless, those EOs richer in camphor
are of interest in aromatherapy and phytotherapy (Herraiz-Peñalver et al. 2013).
Traditionally, spike lavender is used as a raw material in perfumery and cosmet-
ics due to the olfactory properties of its essential oil that contains the monoterpenes
camphor, cineol, and linalool as major constituents (Aprotosoaie et al. 2017 and
references therein). In addition, this oil exerts major effects on the central nervous
system (anxiolytic, sedative, anticonvulsant, analgesic, local anesthetic activity),
and it also shows antioxidant, antimicrobial, anti-inflammatory, spasmolytic, and
carminative properties, which render it highly appreciated in phytotherapy and aro-
matherapy (Cavanagh and Wilkinson 2002; El Alaoui et al. 2017; Manion and
Widder 2017; Woronuk et al. 2011). Several investigations also report on the bio-
cide action of plant essential oils, including those from lavender, which could poten-
tiate its use as eco-friendly pesticides (Haig et al. 2009; Gómez-Mateo et al. 2016;
Varona et al. 2010).
304 J. Segura et al.
11.2 M
etabolic Pathways for the Biosynthesis of Essential
Oil Constituents in the Lavandula Genus
As previously stated, mono- and sesquiterpenes (the C10 and C15 isoprenoids,
respectively) are the major fractions of lavender genus EOs. The biosynthesis of
these compounds is conceptually divided into four stages (Lange and Ahkami
2013).
Stage 1 encompasses the synthesis of the universal C5 terpene precursor isopen-
tenyl diphosphate (IPP) and its allylic isomer dimethylallyl diphosphate (DMAPP),
which in plants are produced via two compartmentalized pathways (Fig. 11.2;
Rodríguez-Concepción and Boronat 2002; Vranová et al. 2013; Zebec et al. 2016).
One is the methyl-D-erythritol 4-phosphate pathway that starts with the biosynthesis
11 Biotechnological Approaches to Increase Essential Oil Yield and Quality… 305
Fig. 11.2 Overview of the biosynthesis of isoprenoids in plants. The following enzymes are indi-
cated in boldface: DXR, 1-deoxy-D-xylulose-5-phosphate reductoisomerase; DXS, 1-deoxy-D-
xylulose-5-phosphate synthase; FPPS, farnesyl diphosphate synthase; GGPPS, geranylgeranyl
diphosphate synthase; GPPS, geranyl diphosphate synthase; HMGR, 3-hydroxy-3-methylglutaryl
CoA reductase; SQS, squalene synthase; STS, sesquiterpene synthase. Enzymes catalyzing mono-
terpene synthesis are indicated by numbers: (1) linalool dehydratase-hydrolase; (2) geraniol
hydroxy-mutase; (3) myrcene synthase; (4) S-linalool synthase; (5) geraniol synthase; (6) cineole
synthase; (7) bornyl diphosphate synthase; (8) bornyl diphosphate diphosphatase; (9) borneol
dehydrogenase; (10) limonene synthase. The first intermediate specific to each pathway is boxed:
DMAPP, dimethylallyl diphosphate; DXP, 1-deoxy-D-xylulose-5-phosphate; FPP, farnesyl
diphosphate; GGPP, geranylgeranyl diphosphate; GPP, geranyl diphosphate; G3P,
D-glyceraldehyde-3-phosphate; HMG-CoA, 3-hydroxy-3-methylglutaril-CoA; IPP, isopentenyl
diphosphate; MEP, methyl-D-erythritol-4-phosphate; MVA, mevalonic acid. Pathways inhibitors:
MEV, mevinoline; FSM, fosmidomycin
11.3 M
etabolic Engineering of Monoterpene Biosynthesis
in Spike Lavender
The ways in which the two terpene synthesis pathways can be well regulated are not
yet unraveled (Banerjee and Sharkey 2014; Daviet and Schalk 2010; Rodríguez-
Concepción 2006; Vranová et al. 2013). It is known that at least three enzymes
(DXS, DXR, and HDR) are involved in plastidial pathway (MEP) regulation
(Córdoba et al. 2009; Rodríguez-Concepción 2006). The results obtained for both
plants and bacteria indicate that the HDR and DXS enzymes mainly control the flux
through the said pathway, both at transcriptional and post-transcriptional level in
response to out-cellular signals of different nature: metabolic, developmental, or
environmental (for review see Córdoba et al. 2009; Rodríguez-Concepción 2006).
The role of DXR, responsible for the conversion of DXP into MEP, remains unclear
in regard to the regulation of the MEP pathway. Hence, although DXR activity in
bacteria did not limit isoprenoid biosynthesis, in plants their regulatory role appears
to be species dependent (Rodríguez-Concepción 2006). Furthermore, the MVA
pathway is mainly controlled at the HMGR level (Enfissi et al. 2005); it is acknowl-
edged that HMGR activity regulates both the flux through the MVA pathway and
the eventual production of isoprenoid end products (Leivar et al. 2011; Rodríguez-
Concepción 2006). A recent investigation supports the role of mevalonate kinase
(PMK) as an unsuspected regulatory hub in the plant MVA pathway and hint at a
role of isopentenyl phosphate (IP) in regulating the formation of both MVA and
MEP pathway-derived terpenoids (Henry et al. 2018).
To elucidate the role of DXS, DXR, and HMGR enzymes in the biosynthesis of
monoterpenes in spike lavender, all three enzymes have been overexpressed sepa-
rately. The DXS (Muñoz-Bertomeu et al. 2006), DXR (Mendoza-Poudereux et al.
2014a), and HMGR (Muñoz-Bertomeu et al. 2007b) in Arabidopsis thaliana (L.)
Heynh genes, under the control of the constitutive CaMV 35S promoter (Fig. 11.3;
constructions kindly provided by Professor A. Boronat, University of Barcelona,
Fig. 11.3 Schematic diagram of the T-DNA constructions employed for genetic transformation of
Lavandula latifolia using the protocol previously developed by our group (Nebauer et al. 2000).
(a) pLBI1DXSBS1, (b) pLBI1DXSR10, and (c) pBICD1 containing a neomycin phosphotransfer-
ase II (nptII) marker gene and a truncated form of the Arabidopsis (A. thaliana) HMG1 cDNA. All
the constructions were kindly provided by Professor A Boronat (University of Barcelona, Spain)
308 J. Segura et al.
Spain), were transferred into the spike lavender genome. For that, the spike lavender
leaf transformation protocol previously described (Nebauer et al. 2000) was fol-
lowed. T0 and their respective self-derived T1 progenies were carefully character-
ized, by monitoring for each transgene the copy number and the expression level of
the genes, the essential oil profile, and even the photosynthetic pigment content.
11.3.1.1 U
pregulation of DXS Increases Essential Oil Production in Spike
Lavender Without Apparent Detrimental Effects on Plant
Development and Fitness
Transgenic T0 plants overexpressing the DXS gene increased their essential oil pro-
duction from 1.2- to 1.7-fold in flowers and from 2.0- to 4.6-fold in leaves, as com-
pared to controls (Fig. 11.4). Analysis performed on self-derived T1 progenies
revealed Mendelian segregation ratios of the DXS transgene (data not shown).
Besides, T1 plants inheriting the DXS transgene had significantly increased essen-
tial oil yield. The correlation was especially apparent when comparing in develop-
ing leaves DXS transgene expression levels and monoterpene content (Fig. 11.5). In
addition, overexpression of DXS transgene in spike lavender leads to increased
Fig. 11.4 Essential oil yield increase (fold-change) in leaves and flowers of control and transgenic
T0 spike lavender plants transformed with Arabidopsis DXS gene. Data represent means ± SD of
four measurements. (Adapted from Muñoz-Bertomeu et al. 2006)
11 Biotechnological Approaches to Increase Essential Oil Yield and Quality… 309
Fig. 11.5 Essential oil yield (mg/g dried weight) from leaves of representative transgenic T1 spike
lavender plants obtained from controlled self-pollination of T0 transgenic DXS1, DXS4, and
DXS6 lines. Reported values for each T1 plant represent the mean ± SD of four measurements.
DXS1-8 and DXS1-9, DXS4-5 and DXS4-6, and DXS6-8 and DXS6-9 are T1 plants that did not
inherit the DXS transgene. Northern blotting tests of DXS gene for each progeny are also shown.
The expression of the TUB3 gene is shown to verify equal loading. (Adapted from Muñoz-
Bertomeu et al. 2006)
linalool content in essential oil from flowers but not from leaves (Muñoz-Bertomeu
et al. 2006).
The results available show that metabolic flux control through the MEP pathway
differs between spike lavender and peppermint, another economically important
Lamiaceae plant species. Thus, upregulation of DXS in L. latifolia led to the highest
improved essential oil production reported thus far in this species (up to 74% in
flowers and up to 359% in leaves (Muñoz-Bertomeu et al. 2006). In contrast, upreg-
ulation of the same enzyme in peppermint did not result in significant increase in
essential oil production in any of the 28 transgenic lines produced (Lange et al.
2011).
Volatile terpenoid metabolic engineering can inflict an expense on plant fitness
and growth, caused by the reduced resource of primary metabolism precursors
(Aharoni et al. 2005 and references therein). This is apparently not the case, how-
ever, for spike lavender since the photosynthetic pigment content did not differ sig-
nificantly in DXS overexpressing transgenic and control plants. This means DXS
overexpression did not increase the MEP-derived photosynthetic pigments. These
findings imply that constitutive DXS overexpression does not translate into equally
high DXS activity in all the tissues, organs, and even cellular types in the plant
(Muñoz-Bertomeu et al. 2006). As reported by Guevara-García et al. (2005), the
MEP pathway is regulated at different levels, both transcriptional and post-
transcriptional. This provides an explanation for which DXS overexpression in L.
latifolia raises EO yield in flower and leaf glandular trichomes without increasing
310 J. Segura et al.
the yield of other terpene-derived compounds in other cell tissues, such as chloro-
phylls and carotenoids in photosynthetic cells. This makes the DXS gene an interest-
ing target for biotechnological interventions for increasing the total yield in this
plant species.
11.3.1.2 D
XR is Not a Rate-Determining Enzyme for Essential Oil
Production in Spike Lavender
DXR enzyme catalyzes the first committed step of the MEP pathway (Carretero-
Paulet et al. 2002). When it was first approached, it was expected that upregulation
of this enzyme in L. latifolia would lead to increased amounts of essential oil, rep-
licating previously accomplished results by overexpressing the DXS gene (Muñoz-
Bertomeu et al. 2006; see Sect. 11.3.1.1). This was not the general effect in most of
the T0 lines (Mendoza-Poudereux et al. 2014a). Only two out of the seven trans-
genic T0 spike lavender plants that were analyzed produced more essential oils than
controls; nevertheless, these increased essential oil phenotypes are hardly imputable
to the DXR transgene effect since correlation between transcript accumulation and
monoterpene production could not be established in T0 (Fig. 11.6; Mendoza-
Poudereux et al. 2014a). Specifically, the DXR2 line showed an increase in the
essential oil content in leaves (2.0-fold for young leaves and 1.3-fold for mature
leaves) and flowers (1.3-fold) and line DXR5 also showed a higher essential oil
production than controls but only in the youngest leaves (1.6-fold). Similar results
Fig. 11.6 (a) Essential oil yield (mg/g fresh weight) in leaves from Set I (first and second whorls)
and Set II (third whorl) of control and transgenic T0 DXR and DXS spike lavender plants. Reported
values represent the means ± SD of three measurements. Northern blotting tests of DRS gene for
each set and gel-loading control are also shown. (b) Detail of the first to tenth whorl leaves grouped
in five developmental stages (Set I–V) used in the experiments (Mendoza-Poudereux et al. 2014a)
11 Biotechnological Approaches to Increase Essential Oil Yield and Quality… 311
were obtained when total chlorophyll and carotenoid content in both T0 transgenic
plants and their progenies were analyzed (Mendoza-Poudereux et al. 2014a). These
results imply that DXR enzyme does not play a crucial role in the synthesis of plas-
tidial monoterpene precursors and may not be used efficiently for biotechnological
purposes. This leaves the DXS enzyme as the main controls point of the flux through
the MEP pathway in L. latifolia. This is in sharp contrast to what was actually
reported for peppermint, where constitutive expression of the DXR gene lead to a
generalized increase in essential oil yield (Mahmoud and Croteau 2001), which cor-
roborates the differences mentioned earlier in the control of metabolic flux trough
the MEP pathway in both Lamiaceae species.
11.3.1.3 U
pregulation of HMGR Increases Essential Oil Yields as Well
as End Product Phytosterols
As stated in Sect. 11.3.1.1, the upregulation of DXS in spike lavender plants was
followed by boosted monoterpene levels. While these findings provide confirmation
to the contribution of the MEP pathway in the synthesis of EOs in this species, the
possibility that the MVA pathway could provide EOs precursors is not excluded.
Likewise, metabolic cross-talk between both the terpene synthesis pathways is well
documented (Henry et al. 2018; Vranová et al. 2013 and references therein). To
investigate the potential role of the MVA pathway in the synthesis of EOs in L. lati-
folia, a truncated form of the A. thaliana HMG1 cDNA was introduced and overex-
pressed in this species (Fig. 11.3) encoding the catalytic domain of the HMGR1S
isoform (Muñoz-Bertomeu et al. 2007b). As shown in Fig. 11.7, upregulation of
HMGR1S improved EO production. Monoterpenes constituted the largest percent-
age of the oil production in transgenic L. latifolia plants, but sesquiterpenes pre-
sented the top increases when compared to controls, with average increases of
1.4- versus 1.8-fold (flowers) and 1.5- versus 3.0-fold (leaves). It is known that
sesquiterpenes are formed in the cytoplasm, which could explain this differential
behavior (Dudareva et al. 2006). Besides, offspring that inherited the HMG1 trans-
gene had significantly increased EO production (data not shown). All these findings
apparently support the contribution of the MVA pathway in some form to the bio-
synthesis of EO in L. latifolia.
As expected, the HMGR1S overexpression also enhanced the sterol amount, spe-
cifically stigmasterol and β-sitosterol, in transgenic T0 spike lavender plants
(increased averages of almost twofold). This increased sterol phenotype of trans-
genic T0 L. latifolia plants was inherited by their progenies, which confirms that
flux leading to stigmasterol and β-sitosterol was under HMGR control (Fig. 11.8).
Furthermore, HMGR1S overexpression had no effect on the photosynthetic pig-
ments’ (chlorophylls and carotenoids) content in those plants (data not shown), con-
firming previous findings on terpene pathways compartmentalization in
photosynthetic tissues (Lichtenthaler 1999).
Taking all these findings, we hypothesized that in L. latifolia there is tissue/
organ-dependent metabolic cross-talk between MEP and MVA pathways, possibly
312 J. Segura et al.
Fig. 11.7 Essential oil yield increase (fold-change) in leaves and flowers of control and transgenic
T0 spike lavender plants transformed with the Arabidopsis HMG1 gene. Reported values represent
the mean ± SD of four measurements. (Adapted from Muñoz-Bertomeu et al. 2007b)
To investigate whether the MVA pathway might be a source of C5 units to the plas-
tids for monoterpene synthesis, both inhibitor-based and 13C labeling experiments
were performed using L. latifolia seedlings (Mendoza-Poudereux et al. 2015).
Particularly, metabolic fluxes of both terpene synthesis pathways were disturbed
11 Biotechnological Approaches to Increase Essential Oil Yield and Quality… 313
Fig. 11.8 Sterol content (microgram per gram-dried weight) from leaves of representative trans-
genic T1 spike lavender plants obtained from controlled self-pollination of T0 transgenic HMGR2
and HMGR4 lines. HMGR2-5 and HMGR2-6 are T1 plants that did not inherit the HMG1 trans-
gene. Reported values for each T1 plant represent the mean ± SD of four measurements. Northern
blotting tests of HMG1 gene for each progeny are also shown. The expression of the TUB3 gene is
shown to verify equal loading. (Adapted from Muñoz-Bertomeu et al. 2007b)
314 J. Segura et al.
with fosmidomycin (FSM) or mevinolin (MEV), specific inhibitors of the MEP and
MVA pathways, respectively (Fig. 11.2). MEV competitively inhibits the HMGR
enzyme, while FSM is an inhibitor of the DXR enzyme; both inhibitors have been
used in terpene metabolism research before (Bach and Lichtenthaler 1983; Re et al.
1995; Rodríguez-Concepción 2006). Moreover, 13C-labeling experiments using
[U-13C6] glucose tracer were performed, attempting to measure the relative contri-
butions of MEP and MVA pathways in monoterpene synthesis (camphor and cineol)
of wild-type and HMGR overexpressing transgenic plants.
The results showed that MEV concentrations higher than 0.5 mM significantly
reduced plant development, but left the photosynthetic pigment or EO synthesis
untouched. Instead, FSM concentrations higher than 20 mM blocked the synthesis
of photosynthetic pigments or EO and consequently reduced stem development
(Mendoza-Poudereux et al. 2015). Mevalonate (MVA) recovered the normal pheno-
type of plants treated with MEV. Furthermore, in FSM-treated sprouts, MVA par-
tially restored the biosynthesis of photosynthetic pigments (carotenoids and
chlorophylls) and, less so, of EOs (Fig. 11.9). These findings suggest that some of
Fig. 11.9 Phenotype recovery with increasing concentrations of MVA (0.0, 0.3, 0.6, 1.2, 2.4, or
3.5 mM) in WT spike lavender shoot apices grown in the presence of 1 mM MEV (top) or 30 μM
FSM (bottom). (Adapted from Mendoza-Poudereux et al. 2015)
11 Biotechnological Approaches to Increase Essential Oil Yield and Quality… 315
the IPP flux produced by the cytosolic pathway may be transferred to the chloro-
plast and be used for the biosynthesis of carotenoids and chlorophylls in L. latifolia
as previously reported in Arabidopsis and tobacco (Hemmerlin et al. 2003; Nagata
et al. 2002).
To give a more accurate view of the possible cross-talk between MEP and MVA
pathways, labeling experiments in which spike lavender transgenic HMGR5 and
control plants were grown on medium containing 2 g/L [U-13C6] glucose and 30 g/L
of unlabeled sucrose for 7, 14, 21, or 28 days were performed. After the feeding
periods, leaf EO was extracted with chloroform and analyzed by GC/MS. Camphor
and 1,8-cineole, the most abundant monoterpenes in the spike lavender leaf EO
(Muñoz-Bertomeu et al. 2007b), were chosen as the reference monoterpenes to esti-
mate the relative carbon flux through both terpenes pathway. GC/MS analysis of
these monoterpenes indicated that their C5-precursors (IPP and DMAPP) are pre-
dominantly biosynthesized via the MEP pathway. However, based on the isotopo-
logue profiles, a contribution of the MVA pathway, although minor, was patent. This
contribution was bigger in transgenic spike lavender plants overexpressing the
HMGR enzyme than in the controls. Both the inhibitor treatments and labeling
experiments provide evidence for transport of MVA-derived precursors from the
cytosol to the plastids in leaves of spike lavender (Mendoza-Poudereux et al. 2015).
11.3.2 O
verexpression of Terpene Synthases Modifies the Leaf
Essential Oil Profile in Spike Lavender
To test if the manipulation of the final steps of the monoterpene biosynthetic path-
way can be used to modify essential oil profile, the monoterpene limonene (LS) and
linalool (LIS) synthases were upregulated in spike lavender (Mendoza-Poudereux
et al. 2014b; Muñoz-Bertomeu et al. 2008). Limonene and linalool, the respective
products of the catalytic activity of these enzymes (Fig. 11.2), are implicated dis-
tinctively in the biological properties and quality of the spike lavender essential oil.
11.3.2.1 E
xpression of Spearmint Limonene Synthase in Transgenic
Spike Lavender Alters Monoterpene Composition in Developing
Leaves
Spearmint (Mentha spicata L.) limonene synthase (MsLS) gene, provided by Prof.
Croteau (Washington State University, Pullman, USA), was constitutively expressed
in spike lavender (Muñoz-Bertomeu et al. 2008). LS enzyme catalyzes the conver-
sion of geranyl diphosphate into limonene (Fig. 11.2). This LS transgene overexpres-
sion did not consistently affect EO profile from flowers or mixed leaves from fourth
to tenth whorls (Muñoz-Bertomeu et al. 2008). It is known that leaf age influences
production of monoterpenes in some aromatic species (Dudai et al. 2001; Gershenzon
et al. 2000; Turner et al. 2000). Therefore, an experiment using leaves sampled at
different developmental stages was designed and completed. EO increase was higher
in developing than in mature leaves in all plants (data not shown). The contents of the
most common monoterpenes (borneol, camphor, cineole, limonene, linalool, myr-
cene, α-pinene, and α-terpineol) found in L. latifolia oils were increased in develop-
ing leaves from the first whorl and decreased over the leaves’ lifespan. The content
of the sesquiterpene trans-caryophyllene followed the same pattern, but that of its
oxygenated derivative increased with leaf age. This robust developmental regulation
of mono- and sesquiterpene biosynthesis was reproducibly observed in all transgenic
progenies, irrespective of LS transgene inheritance (Muñoz-Bertomeu et al. 2008).
Figure 11.10 shows the limonene content in T0 leaves at different developmental
stages; it is worth noting that the limonene increase in the youngest transgenic
leaves reached up to a 450% compared to control plants, which also matched with
the highest transcript accumulation of the LS gene. Also, and as expected, LS over-
expression did not change the chlorophyll and carotenoid content in L. latifolia
leaves (Muñoz-Bertomeu et al. 2008).
In brief, these results show that spearmint LS gene overexpression can modify
monoterpene profile in L. latifolia oil. The detected alterations in EO composition of
the LS transgenic plants are age regulated, being especially evident in young leaves.
Fig. 11.10 Effect of leaf developmental stage (see Fig. 11.6b) on the production (microgram per
gram fresh weight) of limonene in essential oil of controls and T0 transgenic LS6 and LS7 spike
lavender lines. Reported values represent the mean ± SD of four measurements (Muñoz-Bertomeu
et al. 2008)
11 Biotechnological Approaches to Increase Essential Oil Yield and Quality… 317
11.3.2.2 E
xpression of Linalool Synthase in Transgenic Spike Lavender
Alters Monoterpene Composition in Developing Leaves
Essential oils with high linalool content and low camphor content are preferred
for the perfume and cosmetic industries (Dušková et al. 2016). In addition, lin-
alool is a critical precursor for vitamin E, vitamin A, farnesol, citronellol, and
ionones and also has antifungal, antimicrobial, and insecticidal properties
(Aprotosoaie et al. 2014; Beier et al. 2014; Herman et al. 2016). However, the
monoterpene profile of spike lavender essential oil has high relative amounts of
camphor and cineole at the expense of linalool and linalyl acetate, which results
in a more pungent scent (Lis-Balchin 2002). Consequently, from an economical
point of view, the biotechnological breeding of spike lavender is required to
improve the quality of its EO by increasing the ratio of linalool to camphor.
The linalool synthase (LIS) gene from Clarkia breweri (A. Gray) Greene, pro-
vided by Professor Pichersky (University of Michigan, USA), was constitutively
expressed in spike lavender, and the T0 transgenic plants obtained showed a signifi-
cant increase in linalool content as compared to control (Mendoza-Poudereux et al.
2014b). The positive effect of LIS transgene was particularly striking in the y oungest
leaves of two transgenic lines, where linalool increased up to a 1000% (Fig. 11.11).
This high linalool-producing phenotype was maintained in leaf EO of the progenies
that inherited the transgene (Fig. 11.12).
Fig. 11.11 Effect of leaf developmental stage (Set I and II, see Fig. 11.6B) on the production
(microgram per gram fresh weight) of linalool in essential oil of controls and T0 transgenic LIS
spike lavender lines. Reported values represent the mean ± SD of at least three measurements.
(Adapted from Mendoza-Poudereux et al. 2014b)
318 J. Segura et al.
Fig. 11.12 Effect of leaf developmental stage (Sets I–V, see Fig. 11.6b) on the linalool content
(microgram per gram of fresh weight) in spike lavender essential oils from transgenic T0 LIS6 and
some of their T1 progenies. LIS6-12 T1 progeny did not inherit the LIS transgene. Reported values
represent the mean ± SD of at least three measurements. (Adapted from Mendoza-Poudereux et al.
2014b)
Interestingly, EO from these transgenic spike lavender flowers did not show the
increased linalool phenotype found in the leaves. Linalool content is the main dif-
ference between leaf and flower spike lavender EO (traces in leaves and more than
15% of the total oil in flowers), which suggest a strong spatial regulation of the LIS
enzyme as has been reported for other monoterpene synthases (Dudareva et al.
2004; Irmisch et al. 2012; Tholl 2006). Based on our results, we hypothesize that
the LIS transgene only increases linalool content in organs with a low LIS activity
(leaves). This is in accordance with previous studies in other plants, where the lack
of linalool increase was due to its conversion into another compound in order to
store it or as a side effect of the normal metabolism of monoterpenes in plants
(Lavy et al. 2002; Lewinsohn et al. 2001; Lücker et al. 2001). A statistical limita-
tion due to the availability of IPP might also be the cause for this phenomenon in
flowers.
As stated in Sect. 11.3.2.2, the overexpression of the LIS gene changes the monoter-
pene profile of the spike lavender leaf EO by significantly increasing its linalool
content. However, a clear correlation between the overexpression of this gene and
11 Biotechnological Approaches to Increase Essential Oil Yield and Quality… 319
the leaf EO yield could not be established (Mendoza-Poudereux et al. 2014b).
Since an increased EO yield in spike lavender is dependent on the C5 unit precursors
supply, we undertook controlled crosses to generate double transgenic spike laven-
der plants that contained both the DXS gene, whose overexpression significantly
increases EO content in spike lavender (Muñoz-Bertomeu et al. 2006) and the LIS
gene (Mendoza-Poudereux et al. 2014b). The analysis of the leaf EO showed, how-
ever, that both linalool content and EO yield from double transgenic plants were
significantly lower than that obtained in the DXS6 mother plant (Fig. 11.6). Northern
blotting of double transgenic plants showed that transcript levels of both DXS and
LIS genes were minimal. Because of this, the low EO yield and the reduction in the
linalool content of double transgenic plants as compared to the DXS6 mother plant
could be explained by assuming effects of co-suppression of the transgenes, as has
been already observed in other plant species (Baulcombe 2004; Kanazawa 2008).
Thus, the generation of double transgenic spike lavender plants overexpressing both
DXS and LIS genes does not appear to be an appropriate strategy for the biotechno-
logical improvement of spike lavender since neither linalool content nor EO yield
could be improved Fig. 11.13.
This review demonstrates the potential of the first step of the MEP pathway, cata-
lyzed by the DXS enzyme, as a site for metabolic engineering of the aromatic spe-
cies spike lavender, where end-product monoterpenes’ yield is enhanced by
increasing the supply of precursors to specific branches of the isoprenoid pathway.
In contrast, DXR enzyme does not play a crucial role in the synthesis of plastidial
monoterpene precursors in this species. The upregulation of HMGR enzyme also
leads to an increased yield of essential oil in spike lavender, suggesting that MVA
pathway also provides C5 precursors for the biosynthesis of monoterpenes. Specific
inhibitors and 13C-labeling experiments using [U-13C6] glucose partially support this
cross-talk between both pathways.
The constitutive overexpression of either LS or LIS genes in spike lavender, in
which limonene and linalool are minor components of the leaf essential oil, leads to
significant increases in the accumulation of these monoterpenes, especially in young
leaves, which also indicates a good correlation between LS or LIS transcript levels
and limonene or linalool accumulation.
Given the commercial significance of Lavandula latifolia for pharmaceutical,
cosmetic, and food industries, the results reviewed here provide useful informa-
tion for improved breeding. Besides the biotechnologically relevant enhancement
in the yield of spike lavender oil, plants stably overexpressing genes of MEP and
MVA pathways as well as specific monoterpenes synthases provide a valuable
model for studying the monoterpene biosynthesis pathways and their regulatory
mechanisms.
320 J. Segura et al.
Fig. 11.13 Linalool content (microgram per gram of fresh weight) in pooled leaves from first to
third whorls (see Fig. 11.6b) in DXS6 parental and double transgenic DXS6-LIS8 spike lavender
plants. WT, wild type plant; IC, internal control (progeny that did not inherit any of the genes).
Reported values represent the mean ± SD of three measurements. Expression analysis of the DXS
and LIS transgenes in pooled leaves from first to third whorls (see Fig. 11.6b) and gel-loading
control are also shown. (Adapted from Mendoza-Poudereux et al. 2014b)
CRISPRi system successfully modulated the expression of all the MVA pathway
genes in the context of operon and blocked the transcription of the acetoacetyl-CoA
thiolase enzyme that catalyzes the first step in the MVA pathway (Kim et al. 2016).
The authors conclude that CRISPRi is revealed as a robust tool for systematic mod-
ulation of biosynthetic and endogenous gene expression. Thus, it might be used to
tune the metabolic pathway for the biosynthesis of monoterpenes in spike lavender.
Here we have presented three genes (DXS, HMGR, and LIS) that could be targeted
to increase the amount and quality of spike lavender EOs and two other genes (DXR
and LS) that could be in principle avoided for any new interventions regarding
enhancing monoterpene production, easing the new steps into the metabolic engi-
neering of spike lavender.
References
Abbas F, Ke Y, Yu R, Yue Y, Amanullah S, Jahangir MM, Fan Y (2017) Volatile terpenoids: mul-
tiple functions, biosynthesis, modulation and manipulation by genetic engineering. Planta
246:803–816
Aharoni A, Jongsma MA, Bouwmeester HJ (2005) Volatile science? Metabolic engineering of
terpenoids in plants. Trends Plant Sci 10:594–602
Aprotosoaie AC, Hăncianu M, Costache II, Miron A (2014) Linalool: a review on a key odorant
molecule with valuable biological properties. Flavour Fragr J 29:193–219
Aprotosoaie AC, Gille E, Trifan A, Luca VS, Miron A (2017) Essential oils of Lavandula genus: a
systematic review of their chemistry. Phytochem Rev 16:761–799
Arimura G, Matsui K, Koeduka T, Holopainen JK (2017) Biosynthesis and regulation of plant vol-
atiles and their functional roles in ecosystem interactions and global environmental changes.
In: Arimura G, Maffei M (eds) Plant specialized metabolism: genomics, biochemistry, and
biological functions. CRC Press, Taylor & Francis Group, Boca Raton, pp 185–238
Ashour M, Wink M, Gershenzon J (2010) Biochemistry of terpenoids: monoterpenes, sesquiter-
penes and diterpenes. In: Wink M (ed) Biochemistry of plant secondary metabolism. Annu
Plant Rev 40. Wiley-Blackwell, Oxford, pp 258–303
Bach TJ, Lichtenthaler HK (1983) Inhibition by mevinolin of plant growth, sterol formation and
pigment accumulation. Physiol Plant 9:50–60
Banerjee A, Sharkey TD (2014) Methylerythritol 4-phosphate (MEP) pathway metabolic regula-
tion. Nat Prod Rep 31:1043–1055
Baulcombe D (2004) RNA silencing in plants. Nature 431:356–363
Beier RC, Byrd JA, Kubena LF, Hume ME, McReynolds JL, Anderson RC, Nisbet DJ (2014)
Evaluation of linalool, a natural antimicrobial and insecticidal essential oil from basil: effects
on poultry. Poult Sci 93:267–272
Campos N, Boronat A (1995) Targeting and topology in the membrane of plant 3-hydroxy-3-
methylglutaryl coenzyme A reductase. Plant Cell 7:2163–2174
Carretero-Paulet L, Ahumada I, Cunillera N, Rodríguez-Concepción M, Ferrer A, Boronat A,
Campos N (2002) Expression and molecular analysis of the Arabidopsis DXR gene encoding
322 J. Segura et al.
Mahmoud SS, Croteau RB (2002) Strategies for transgenic manipulation of monoterpene biosynthesis
in plants. Trends Plant Sci 7:366–373
Manion CR, Widder RM (2017) Essentials of essential oils. Am J Health Syst Pharm 74:e153–e162
McGarvey DJ, Croteau R (1995) Terpenoid metabolism. Plant Cell 7:1015–1026
Mendoza-Poudereux I, Muñoz-Bertomeu J, Arrillaga I, Segura J (2014a) Deoxyxylulose
5-phosphate reductoisomerase is not a rate-determining enzyme for essential oil production in
spike lavender. J Plant Physiol 171:1564–1570
Mendoza-Poudereux I, Muñoz-Bertomeu J, Navarro A, Arrillaga I, Segura J (2014b) Enhanced
levels of S-linalool by metabolic engineering of the terpenoid pathway in spike lavender leaves.
Metab Eng 23:136–144
Mendoza-Poudereux I, Kutzner E, Huber C, Segura J, Eisenreich W, Arrillaga I (2015) Metabolic
cross-talk between pathways of terpenoid backbone biosynthesis in spike lavender. Plant
Physiol Biochem 95:113–120
Muñoz-Bertomeu J, Arrillaga I, Ros R, Segura J (2006) Up-regulation of 1-deoxy-D-xylulose-5-
phosphate synthase enhances production of essential oils in transgenic spike lavender. Plant
Physiol 142:890–900
Muñoz-Bertomeu J, Arrillaga I, Segura J (2007a) Essential oil variation within and among natural
populations of Lavandula latifolia and its relation to their ecological areas. Biochem Syst Ecol
35:479–488
Muñoz-Bertomeu J, Arrillaga I, Ros R, Segura J (2007b) Up- regulation of an N-terminal truncated
3-hydroxy-3-methylglutaryl CoA reductase enhances production of essential oils and sterols in
transgenic Lavandula latifolia. Plant Biotech J 5:746–758
Muñoz-Bertomeu J, Ros R, Arrillaga I, Segura J (2008) Expression of spearmint limonene syn-
thase in transgenic spike lavender results in an altered mono-terpene composition in developing
leaves. Metab Eng 10:166–177
Nagata N, Suzuki M, Yoshida S, Muranaka T (2002) Mevalonic acid partially restores chloro-
plast and etioplast development in Arabidopsis lacking the non-mevalonate pathway. Planta
216:345–350
Nebauer SG, Arrillaga I, del Castillo-Agudo L, Segura J (2000) Agrobacterium tumefaciens-
mediated transformation of the aromatic shrub Lavandula latifolia. Mol Breed 6:539–552
Pichersky E, Gershenzon J (2002) The formation and function of plant volatiles: perfumes for pol-
linator attraction and defense. Curr Opin Plant Biol 5:237–243
Re EB, Jones D, Learned RM (1995) Co-expression of native and introduced genes reveals cryptic
regulation of HMG-CoA reductase expression in Arabidopsis. Plant J 7:771–784
Renaud ENC, Charles DJ, Simon JE (2001) Essential oil quantity and composition from 10 culti-
vars of organically grown Lavender and Lavandin. J Essent Oil Res 13:269–273
Rivas-Goday S, Rivas-Martínez S (1967) Matorrales y tomillares de la Península Ibérica compren-
didos en la clase Ononido-Rosmarinetea Rr. – Bl. 1947. Anal Inst Bot Cavanilles 25:1–183
Rivas-Martínez S (1979) Brezales y jarales de Europa occidental (Revisión Fitosociológica de las
clases Calluno-ulicetea y Cisto-Lavanduletea). Lazaroa 1:5–127
Rodríguez-Concepción M (2006) Early steps in isoprenoid biosynthesis: multilevel regulation of
the supply of common precursors in plant cells. Phytochem Rev 5:1–15
Rodríguez-Concepción M, Boronat A (2002) Elucidation of the methylerythritol phosphate path-
way for isoprenoid biosynthesis in bacteria and plastids. A metabolic milestone achieved
through genomics. Plant Physiol 130:1079–1089
Rohmer M (2003) Mevalonate-independent methylerythritol phosphate pathway for isoprenoid
biosynthesis. Elucidation and distribution. Pure Appl Chem 75:375–388
Salido S, Altarejos J, Nogueras M, Sánchez A, Luque P (2004) Chemical composition and sea-
sonal variations of spike lavender oil from Southern Spain. J Essent Oil Res 16:206–210
Segura J, Calvo MC (1991) Lavandula spp. (lavender): in vitro culture, regeneration of plants, and
the formation of essential oils and pigments. In: Bajaj YPS (ed) Medicinal and aromatic plants
III, biotechnology in agriculture and forestry, vol 15. Springer-Verlag, Berlin-Heidelberg-New
York, pp 283–310
11 Biotechnological Approaches to Increase Essential Oil Yield and Quality… 325
Smanski MJ, Zhou H, Claesen J, Shen B, Fischbach MA, Voigt CA (2016) Synthetic biology to
access and expand nature’s chemical diversity. Nat Rev Microbiol 14:135–149
Smigielski K, Prusinowska R, Krosowiak K, Sikora M (2013) Comparison of qualitative and quan-
titative chemical composition of hydrolate and essential oils of lavender (Lavandula angustifo-
lia). J Essent Oil Res 25:291–299
Tholl D (2006) Terpene synthases and the regulation, diversity and biological roles of terpene
metabolism. Curr Opin Plant Biol 9:297–304
Tissier A, Morgan JA, Dudareva N (2017) Plant volatiles: Going “In” but not “Out” of trichome
cavities. Trends Plant Sci 22:930–938
Turner GW, Gershenzon J, Croteau RB (2000) Development of peltate glandular trichomes of pep-
permint. Plant Physiol 124:665–679
Tutin TO, Heywood VH, Burges NA, Moore DM, Valentine DH, Walters SM, Webb DA (1972)
Flora europea, vol 3. University Press, Cambridge
Upson T (2002) The taxonomy of the genus Lavandula L. In: Lis-Balchin M (ed) Lavender: the
genus Lavandula. Taylor & Francis, London, pp 2–34
Upson T, Andrews S (2004) The genus Lavandula. Royal Botanic Gardens, Kew
Urwin NAR, Mailer RJ (2008) Oil content and fatty acid profiles of seed oil from the genus
Lavandula. J Am Oil Chem Soc 85:491–492
Varona S, Kareth S, Martín A, Cocero MJ (2010) Formulation of lavandin essential oil with bio-
polymers by PGSS for application as biocide in ecological agriculture. J Supercrit Fluids
54:369–377
Vivaldo G, Masi E, Taiti C, Caldarelli G, Mancuso S (2017) The network of plants volatile organic
compounds. Sci Rep 7:11050
Vranová E, Coman D, Gruissem W (2013) Network analysis of the MVA and MEP pathways for
isoprenoid synthesis. Annu Rev Plant Biol 64:665–700
Wang Q, Reddy VA, Panicker D, Mao HZ, Kumar N, Rajan C, Venkatesh PN, Chua NH,
Sarojam R (2016) Metabolic engineering of terpene biosynthesis in plants using a trichome-
specific transcription factor MsYABBY5 from spearmint (Mentha spicata). Plant Biotechnol
J 14:1619–1632
Woronuk G, Demissie Rheault M, Mahmoud S (2011) Biosynthesis and therapeutic properties of
Lavandula essential oil constituents. Planta Med 77:7–15
Zebec Z, Wilkes J, Jervis AJ, Scrutton NS, Takano E, Breitling R (2016) Towards synthesis of
monoterpenes and derivatives using synthetic biology. Curr Opin Chem Biol 34:37–43
Chapter 12
The Phytochemical Composition,
Biological Effects and Biotechnological
Approaches to the Production of High-
Value Essential Oil from Geranium
Abbreviations
12.1 Introduction
Geraniaceae family plants (Pelargonium sp.) are perennial medicinal and essential
oil-yielding branched herbs, growing in subtropical and temperate climates.
Pelargonium genus comprises more than 750 species, and most of them originated
from Europe and Africa. They have remarkable commercial applications due to
their characteristic essential oil. The essential oil distillate of a high-value
Geraniaceae plant, rose-scented geranium (Fig. 12.1), has a very strong, pleasant
and rosy fragrance with a minty top; therefore, it is used as a substitution of
the expensive rose oil, and is also known as ‘poor man’s rose oil’. The essential oil
of rose-scented geranium was extensively used as a flavouring agent in the food,
cosmetic, perfumery, and pharmaceutical industries. The rose-scented gera-
nium essential oil is also well known for its effectiveness in various health-related
treatments such as aromatherapy and for its antimicrobial properties (Narnoliya
et al. 2017, 2018a; Jadaun et al. 2017). In India, geranium was introduced in the
nineteenth century in the southern climate, and now it grows in different parts of
India. They are also used as ornamental plants, for example, Pelargonium x horto-
rum and Pelargonium graveolens, and grown in gardens and parks to provide a
pleasant fragrance (Ravindra and Kulkarni 2015).
There are four species of geranium which have commercial applications: zonal
geranium (Pelargonium x hortorum), scented geranium (Pelargonium sp.), regal
pelargonium (Pelargonium x domesticum) and regal ivy geranium (Pelargonium
peltatum). The essential oil of geranium is synthesized in specialized cells known as
trichomes. The vegetative and reproductive organs of rose-scented geranium are
reported to have non-glandular and glandular trichomes. Generally, glandular tri-
chomes are the major reservoirs for essential oil (Boukhris et al. 2013; Narnoliya
et al. 2017). More than 200 species of geranium occur naturally, out of which P. gra-
veolens, P. odoratissimum, P. radens, and P. capitatum are more commonly used for
harvesting the essential oil. The hydro-distillation method is commonly used for
extraction of oil from the aerial part, especially leaves and stem. Essential oil of
rose-scented geranium is composed of complex volatile phytochemicals, produced
as secondary metabolites, such as terpenes, esters, aldehydes, ketones, alcohols, and
phenols. Generally, they play a crucial role in ecological adjustment of the plant and
protect it from pathogen and herbivore attacks. Thus, essential oil components are
the key substances of rose-scented geranium for its defence system (Babu and Kaul
2005; Jadaun et al. 2017; Ravindra and Kulkarni 2015).
Geranium oil comprises more than 120 phytoconstituents, which include mono-
terpenes, sesquiterpenes, diterpenes, and low molecular weight aroma compounds.
There are three main components, linalool, citronellol and geraniol, and their esters,
which constitute more than 60% of total essential oil, and they are responsible for
determining its odour. Other components are menthone, nerol, isomenthone, rose
12 The Phytochemical Composition, Biological Effects and Biotechnological… 329
oxides, terpineol, pinene and myrcene (Jadaun et al. 2017; Ravindra and Kulkarni
2015). Thus, terpenes are the major contributors in the essential oil of rose-scented
geranium, and these terpenes are biosynthesized through the terpenoid pathway. It
was less explored in terms of its genomics, transcriptomics, gene expression and
enzyme characterization. Recently, the transcriptomic information of rose-scented
geranium leaf has been reported, which provides a foundation for the molecular
study of primary and secondary metabolism (Narnoliya et al. 2017, 2018a).
Recently, a gene, 1-deoxy-d-xylulose-5-phosphate synthase (DXS) was cloned
from this plant and its recombinant protein was physico-kinetically characterized
and heterologously overexpressed in Withania somnifera to evaluate the effect of
DXS on withanolides (Jadaun et al. 2017).
Hence, recent genomics and functionality studies provided a platform to enhance
the quantity and quality of essential oil. Using modern biotechnological and syn-
thetic biological approaches, with the aid of available biological information, it
could be possible to enhance the productivity of rose-scented geranium’s essential
oil. Herein, we discuss the phytochemical composition of geranium essential oil,
and its biological effects. Further, different biotechnological approaches have also
been explored to enhance the production of high-value essential oil.
Table 12.1 (continued)
Chemical
Category Examples of Chemicals
Aliphatic Acetone; 2-butanone; 2-pentanone; 3-methyl-2-butanone; 2-methyl-3-
ketones pentanone; 4-methyl-2-pentanone; 2-methylcyclopentanone; 3-methylcyclo-
pentanone; 3-metylcyclohexanone; 4-methyl-3-penten-2-one; 2-hexanone;
methylheptenone; 6-methyl-5-hepten-2-one; methyl-3-methylcyclo-pentenyl
ketone
Terpene Menthone; isomenthone
ketones
Sesquiterpene 1,7-Dihydrofurapelargone; furapelargone A; furapelargone B; 7,8
ketones dihydrofurapelargone
Aliphatic Benzaldehyde; ethanol; decanal; 2-methylpropanal; 3-methyl-2-butanal;
aldehydes 3-methylbutanal; 2-furfuraldehyde; nonanal; (E)-2-hexenal
Terpene Geranial; citronellal; neral; photocitrl A; epi-photocitral A; photocitral B;
aldehydes p-menth-1-en-9-al
Terpene oxides Cis-rose oxide; trans-rose oxide; cis-linalool oxide; trans-linalool oxide;
anhydrolinalool oxide; bois-de-rose oxide; nerol oxide
Sesquiterpene Caryophyllene oxide
oxides
Aliphatic acids Formic acid; propionic acid; acetic acid; caprylic acid
Terpene acids 6-oxo-6-7-dihydrocitronellic acid; geranic acid; citronellic acid
Miscellaneous Dimethyl sulphide; eugenol; methyl eugenol; furan; α-agarofuran; juniper
camphor; vetispirans theaspirans; rose furan; epoxy-rose furan
There are several factors, such as environmental conditions (climate, soil, humid-
ity, fertilizer and seasonal variation), genotypic and physiological conditions of
plants and distillation method, which affect the essential oil yield and composition
of phytoconstituents in oil (Rao 2009; Sangwan et al. 2001; Verma et al. 2013;
Cannon et al. 2013). There is also a clear ageing effect on the composition of oil in
geranium, as shown in a report by Rajeswara Rao et al. (1993) that essential oil
yield (1.56%) and geraniol content (34.6%) were the highest in the youngest leaf.
Essential oil yield not only varies with different cultivars, but even the same cultivar
may produce essential oil of altered composition in different seasons. Different cul-
tivars of geranium, such as Bourbon, CIM-Pawan and Kelkar, showed variations in
essential oil production from 0.05% to 0.12%, depending on the season of its culti-
vation (Verma et al. 2014). Oil composition fluctuates with climatic conditions, as
citronellol-, nerol-, geraniol- and menthone-rich oil was obtained from plants grown
in temperate climates of high-altitude regions; on the other side, isomenthone-, lin-
alool-, citronellyl formate-rich oil was found in plants grown in lower altitudes
(Rajeswara Rao et al. 1990).
Apart from climatic conditions, soil conditions also have a significant impact on
the essential oil yield in geranium. An experiment was performed on growing gera-
nium plants under water-stress conditions, and it was observed that essential oil
yield was indirectly proportional to the duration of interval periods of irrigation
(Putievsky et al. 1990). The nearby vegetation in cultivation area also affects oil
yield and composition, as reported earlier that presence and absence of weeds affect
the ratio of phytoconstituents in oil. However, some experiments showed that grow-
ing another crop at a particular distance from geranium crop does not affect the oil
composition up to a significant level, and hence, income can be doubled from the
same land area (Rajeswara Rao and Bhattacharya 1997; Singh et al. 2013). The
phytochemical composition of rose-scented geranium oil is presented in Table 12.1.
There are two types of phytoconstituents present in rose-scented geranium, that is,
terpene (major) and phenylpropanoids (minor). Terpenes are synthesized through
the terpenoid/isoprenoid pathway and phenylpropanoids through the shikimate
pathway.
Terpenes are synthesized by the participation of two pathways, one is the cytosolic
mevalonate (MVA) or classical acetate pathway, and another is the plastidial non-
mevalonate or 2-C-methyl-D-erythritol 4-phosphate (MEP) or glyceraldehyde
phosphate/pyruvate or 1-deoxy-D-xylulose 5-phosphate (DXP) pathway (Fig. 12.2).
12 The Phytochemical Composition, Biological Effects and Biotechnological… 333
Fig. 12.2 The terpene biosynthetic pathway in rose-scented geranium. AACT acetoacetyl-CoA
thiolase/acetyl-CoA acetyltransferase, HMGS hydroxymethylglutaryl-CoA synthase, HMGR
hydroxymethylglutaryl-CoA reductase, MVK mevalonate kinase, PMK phosphomevalonate
kinase, MVD mevalonate diphosphate decarboxylase, DXS 1-deoxy-D-xylulose 5-phosphate
synthase, DXR 1-deoxy-D-xylulose 5-phosphate reductoisomerase, MCT 2-C-methyl-D-
erythritol 4-phosphate cytidylyltransferase, CMK 4-(cytidine 5′-diphospho)-2-C-methyl-D-eryth-
ritol kinase, MDS 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, HDS
(E)-4-hydroxy-3- methylbut-2-enyl diphosphate synthase, HDR (E)-4-hydroxy-3-methylbut
2-enyl diphosphate reductase, IDI isopentenyl-diphosphate delta isomerase, GPPS geranyl
diphosphate synthase, FPPS farnesyl pyrophosphate synthase, GGPPS geranylgeranyl diphos-
phate synthase, MTPS: monoterpene synthase, STPS sesquiterpene synthase, DTPS diterpene
synthase, HMG CoA hydroxymethylglutaryl-CoA, IPP isopentenyl pyrophosphate, DMAPP
dimethylallyl pyrophosphate GA-3P: glyceraldehyde 3-phosphate, DXOP 1-deoxy-D-xylulose-
5-phosphate, MEP 2-C-methyl-d-erythritol-phosphate, CDP-ME 4-(cytidine 5′-diphospho)-2-C-
methyl-d-erythritol, CDP-ME2P 2-phospho 4-(cytidine 5′-diphospho)2-c-methyl-d-erythritol,
ME 2,4 cPP C-methyl-D-erythritol 2,4-cyclodiphosphate, HMBPP 1-hydroxy-2-methyl-2-bute-
nyl 4-diphosphate, GPP geranyl pyrophosphate, FPP farnesyl pyrophosphate, GGPP geranylgera-
nyl pyrophosphate, MVA mevalonic acid
334 L. K. Narnoliya et al.
There are several evidences to prove that the MVA pathway is operated in cytosol,
and endoplasmic reticulum plays a major role in the biosynthesis of sesquiterpenes
(C15), triterpenes (C30), and polyterpenes, while the MEP pathway is dedicated for
synthesis of isoprenes (C5), monoterpenes (C10), diterpenes (C20), and tetrater-
penes (C40). The names of the pathways indicate the name of their first products
such as mevalonic acid and 1-deoxy-D-xylulose 5-phosphate in the MVA and MEP
pathways, respectively. Isopentenyl pyrophosphate (IPP), and dimethylallyl diphos-
phate (DMAPP), generated from both the pathways, function as precursors for
manufacturing a plethora of terpenes. Terpenes are biosynthesized as a result of
several enzymatic reactions catalyzed by terpene synthase enzymes. For many
years, MVA was thought to be solely responsible for the synthesis of terpenes, but
radioactive tracer experiments revealed the discovery of the MEP pathway for ter-
pene biosynthesis (Lichtenthaler 1999; Eisenreich et al. 2004). Although both path-
ways contribute to the biosynthesis of geranium essential oil, the MEP pathway is
predominantly involved, as evident by the higher concentration of monoterpenes
than sesquiterpenes in geranium oil (Jadaun et al. 2017).
The MVA pathway starts with condensation reaction of two molecules of acetyl
CoA, which produces acetoacetyl CoA that further undergoes condensation reac-
tion with another acetyl CoA, and forms 3-hydroxy-3-methyl-glutaryl-CoA (HMG-
CoA) as the product. This HMG-CoA is reduced into mevalonic acid (MVA) by the
action of the enzyme HMG-CoA reductase (HMGR). Mevalonic acid kinase (MVK)
and phosphomevalonate kinase (PMK) further convert MVA into mevalonate
diphosphate through the phosphorylation process. Mevalonate-5-diphosphate
decarboxylase (MPD) catalyses an ATP-coupled decarboxylation reaction for the
production of isopentenyl diphosphate (IPP). IPP can be converted into dimethylal-
lyl diphosphate (DMAPP) by an enzyme, IPP/DMAPP isomerase (IDI) (Fig. 12.2).
Recently, a modified MVA pathway was proposed in which mevalonate-5-phosphate
underwent decarboxylation forming isopentenylphosphate, which can be trans-
formed into isopentenyl diphosphate (IPP) by the enzyme isopentenyl phosphate
kinase (IPK) (Chen and Poulter 2010; Hayakawa et al. 2017).
D-Glyceraldehyde 3-phosphate and pyruvate are the precursor molecules in the
MEP pathway. They condense together and form the first intermediate 1-deoxy-D-
xylulose 5-phosphate (DXP). In the next step, DXP is reductively isomerized by
reducto-isomerase (DXR/IspC) into MEP and subsequently couples with cytidine
5′-triphosphate (CTP), generating methyl erythritol cytidyl diphosphate (CDP-ME)
by CDP-ME synthetase (IspD). Then, CDP-ME is phosphorylated to produce
4-diphosphocytidyl-2-C methyl-D-erythritol-2-phosphate (CDP-MEP). An ATP-
dependent enzyme IspE catalyses this reaction. At the next level, cyclization of
CDP-MEP is performed by IspF, which leads to the generation of 2-C-methyl-D
erythritol-2,4- cyclodiphosphate (MEcPP). Further, IspG catalysed transformation
of MEcPP into 4-hydroxy-3-methylbutenyl 1-diphosphate (HMBPP). The final step
of this pathway is performed by the IspH protein, which generates IPP and DMAPP.
For the synthesis of diverse terpenes, isoprene units are joined together in a head-
to-tail pattern. Geranyl pyrophosphate (GPP), a monoterpene precursor molecule, is
synthesized by the action of the geranyl pyrophosphate synthase (GPPS) enzyme.
12 The Phytochemical Composition, Biological Effects and Biotechnological… 335
Further, addition of one more isoprene unit with GPP by farnesyl pyrophosphate
synthase (FPPS) leads to generation of a sesquiterpene precursor, farnesyl pyro-
phosphate (FPP). Head-to-tail condensation of FPP with IPP produces g eranylgeranyl
pyrophosphate (GGPP), a diterpene. In the next stage, addition of one more IPP
with GGPP forms C25 compounds known as sesterterpene. Interestingly, condensa-
tion of farnesyl pyrophosphate (FPP) is used for the synthesis of the C30 triterpene
compound (squalene), and in a similar fashion, tail-to-tail condensation of geranyl-
geranyl pyrophosphate (GGPP) results in the synthesis of C40 molecules, tetrater-
penes (Narnoliya et al. 2017, 2018b).
The arrangement of carbon molecules in the chain takes place according to the
types of terpenes produced. The most common pattern is cyclization of terpenes,
which takes place through generation of an intermediate carbenium ion. For exam-
ple, heterolysis of the carbon oxygen bond of geranyl pyrophosphate produces gera-
nyl carbocation, and when this carbocation reacts with water, it produces geraniol
and subsequently its oxidation leads to synthesis of citral. Granyl carbocation
undergoes intramolecular electrophilic addition reaction to generate monocyclic
carbocation, which produces limonene after a proton elimination reaction. Further,
many other different kinds of intra- and intermolecular interactions are required for
the production of diversified terpene molecules (Narnoliya et al. 2017, 2018b).
Phenylpropanoids are the aromatic compounds which are synthesized by the shiki-
mate acid pathway, and aromatic amino acids phenylalanine and tyrosine are the
precursors of this pathway. The shikimic acid pathway starts with the joining of
D-erythrose 4-phosphate with phosphoenol pyruvic acid, and shikimic acid is pro-
duced as an intermediate, followed by the generation of chorismate. This choris-
mate is further utilized in the generation of phenylalanine, which is converted into
cinnamic acid, and further, it leads to the generation of multiple types of
phenylpropanoids.
Geranium essential oil possesses significant antibacterial properties. Bigos et al.
(2012) stated that geranium oil possesses a compelling antibacterial property against
clinical isolates of Staphylococcus aureus strain ATCC 433000, which contains
multidrug-resistant capacity. Due to its antibacterial effect and almost zero toxicity,
geranium oil can be used in food processing. During quiche filling, addition of gera-
nium essential oil at different concentrations (250 ppm, 500 ppm and 1000 ppm)
showed significant antibacterial property (Lis-Balchin et al. 1998). Essential oil can
be applied in combination with other oils or with standard drugs, and results of such
combinatorial experiments exhibit unexpectedly higher inhibitory rates. For exam-
ple, when citricidal™ and geranium oil were applied together, this combination
showed a highly inhibitory influence against MRSA (methicillin-resistant S. aureus)
and geranium oil methicillin-sensitive S. aureus (Edwards-Jones et al. 2004).
Besides, S. aureus is also effective against other gram-positive strains like Bacillus
cereus, and Bacillus subtilis (Silva and Fernandes 2010). Carmen and Hancu (2014)
also found that geranium essential oil is able to inhibit the growth of gram-negative
bacteria (E. coli, Pseudomonas aeruginosa, and Proteus mirabilis) as well as gram-
positive bacteria (S. aureus, and Enterococcus faecalis). Surprisingly, geranium oil,
either in free form or in capsulated form, acts as an inhibitory factor against
Mycobacterium sp., the most targeted pathogen of current drug professionals.
Geranium oil is effective against M. abscessus, M. massiliense, M. smegmatis, and
M. avium, even at minimal inhibitory concentrations (MICs) [17.9–35.9 μg/ml]
(Giongo et al. 2015). Along with Mycobacterium sp., geranium oil has potent anti-
microbial activities against other screened pathogenic bacteria such as S. aureus,
Streptococcus, Staphylococcus, Listeria monocytogenes, Pseudomonas aeruginosa,
and Salmonella enteritidis (Giongo et al. 2015).
The insecticidal/antifeedant property of geranium oil was well known for the past
many years against various types of insects (Lis 1996). L-Quisqualic acid
(C5H7N3O5), an excitatory amino acid, isolated from the petals of Pelargonium x
hortorum, displayed a paralytic effect on Japanese beetles (Range et al. 2011).
Essential oil of geranium also exhibits insecticidal properties against the insect
Rhyzopertha dominica at a dose of 50 μl/petri dish, 8.5 cm in diameter (Bouzenna
and Krichen 2013).
A drug, EPs® 7630, was formulated using the root extract of Pelargonium sidoides,
and in Germany, this drug is approved for the treatment of bronchitis. This drug has
immunomodulatory and cytoprotective effects, showing an inhibitory effect on the
interaction of bacteria with its host cell. Simultaneously, it stimulates the respiratory
cells by increasing the ciliary beat frequency of these cells (Moyo and Van Staden
2014). This drug was also tested against various viruses related to respiratory infec-
tions, and interestingly, this drug showed inhibitory effects against many tested
viruses such as influenza A virus strains (H1N1, H3N2), parainfluenza virus, cox-
sackie virus and human coronavirus. However, it was found to be ineffective against
adenovirus, pathogenic avian influenza A virus (H5N1) or rhinovirus (Michaelis
et al. 2011). The cumulative effect of antibacterial and antiviral properties of this
drug makes it an efficient drug against respiratory infections. There are a limited
number of anti-HIV 1 therapies; therefore, the development of drugs against human
immunodeficiency virus (HIV) is a task of global concern. In this direction, the use
of aqueous extract of Pelargonium sidoides root was also examined against HIV 1,
and this extract showed significant anti-HIV 1 activity. The mode of action of this
extract is different from previously cited mechanisms as this extract poses an inhibi-
tory impact on the attachment of HIV 1 to host cells, and this happens due to action
of its phenolic components (Helfer et al. 2014).
2013; Krishnan et al. 2012; Narnoliya et al. 2014; Rastogi et al. 2014). Although
transcriptomic data are available from the Geraniaceae family, such as Geranium
maderense, Pelargonium x hortorum (Zhang et al. 2013), etc., the essential oil path-
way (terpenoid pathway) was deeply explored in a transcriptomic study on rose-
scented geranium (Narnoliya et al. 2017). In this analysis, a total of 78,943 unique
transcripts were reported, out of which 51,802 contigs showed homology-based
functional annotation. Further, putative gene(s) representing terpene, ascorbic acid,
tartaric acid and anacardic acid (2-hydroxy-6-alkylbenzoic acid), biosynthetic path-
ways, hormone metabolism and transcription factors were identified. Transcriptomic
study also helped in investigating 6040 simple sequence repeats (SSRs) in rose-
scented geranium. The genes encoding DXS, 1-deoxy-d-xylulose reductoisomerase
(DXR) and 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) were successfully
cloned, and their expression analysis was conducted in rose-scented geranium.
Further, DXS gene was overexpressed homologously (rose-scented geranium) as
well as heterologously (Withania somnifera), resulting in enhanced production of
the essential oil (Jadaun et al. 2017).
Tartaric acid, a high-value food ingredient, is biosynthesized through the catab-
olism of ascorbic acid via two routes, C2/C3 (via threonic acid) and C4/C5 (via
iodic acid) (Debolt et al. 2007; Loews 1999). Geranium is also able to produce a
significant quantity of tartaric acid, but the plant species specific preference for the
alternative cleavage pathway was unclear. Some reports suggest that geranium fol-
lows the C2/C3 cleavage route, but there are insufficient evidences to prove the
absence of C4/C5 route. In our recent transcriptome analysis, a putative gene
encoding the key regulator of the C4/C5 cleavage reaction, L-idonate-5-
dehydrogenase, was annotated with a notable expression level, stipulating the pos-
sibilities of occurrence in both the C2/C3 and C4/C5 route for tartaric acid
biosynthesis (Narnoliya et al. 2017).
Rose-scented geranium is one of the most important commercial plants of the per-
fumery industry. Geranium is cultivated throughout the world for its good-quality
essential oil with roselike fragrance. The demand for essential oil is flourishing
rapidly due to the increased awareness of its beneficial effects. It is not only use-
ful to the perfumery and cosmetic related activities, but also used in the food and
beverage industries because of its application as food additive without any
known side effects. In the global market, currently, Europe accounts as the major
producer of essential oil, but Asia-Pacific is also emerging as a promising continen-
tal leader in the near future (Dhananjay et al. 2010). The estimated demand of gera-
nium oil is around 250 tons per year. The United States, France, Germany, United
Kingdom, Japan and other European countries represent a good market for gera-
nium oil. The main producers and exporters of geranium oil are China, Egypt,
Algeria, and Morocco. On the differences of the origin of geranium cultivars, they
340 L. K. Narnoliya et al.
are majorly divided into three main categories—Reunion Island, Egyptian or North
African and Chinese. In Reunion Island-type cultivar, the ratio of citronellol and
geraniol is almost 1:1, and the other main components are isomenthone, citronellyl
formate, and guaia-6,9-diene. Although Egyptian-type oil also contains a 1:1 ratio
of citronellol and geraniol, their prominent components are citronellyl formate, iso-
menthone, and 10-epi-eudesmol. Chinese-type oil is dominated by citronellol and
citronellyl formate with low amounts of geraniol. In grading of quality, Reunion
type is followed by Egyptian, and then Chinese cultivars. The price of geranium oil
ranges from $55 kg−1 to $110 kg−1, although it depends on oil quality, origin coun-
try, and market demand, but still, the price is quite high.
In 1952, Morel and Martin for the first time introduced the meristem tip culture
technique in plants during the practice of production of virus-free Dahlia plants.
Afterwards, this technology was used for many ornamental and vegetative plants for
production of disease-free plants, whether they were viral, bacterial or fungal dis-
eases (Van Zaayen et al. 1992; Smith 2013; Mohapatra and Batra 2017). Geranium
is prone to several pathogens, and the most prominent are Xanthomonas campestris
pv. pelargonii, Verticillium dahliae, and Botrytis cinerea. It is necessary to maintain
disease-free stocks of cultivars for achieving good market prices (Dunbar 1990).
Even chemical treatment is not so fruitful to keep plant infection free because
mostly these infections are systemic in nature. Therefore, meristem culture is sug-
gested as a useful technology for production of disease-free plants.
For meristem culture, shoot tips (0.1–0.5 mm) are used as explants for regenera-
tion of plantlets. The plants grown from that meristem explant are further screened
for obtaining pathogen-free plants, and presence of pathogen is tested at different
stages of growth, and it can be done by keeping the regenerating plantlet under
conditions favourable for growth of that pathogen. Modern techniques are more
sensitive for testing pathogen contamination in plants. These techniques are enzyme-
linked immunosorbent assay (ELISA), colorimetric assays, and polymerase chain
reactions (PCR). These techniques are able to detect very low levels of infection.
Several protocols have been standardized for production of disease-free geranium
plants (Debergh and Maene 1977; Reuther 1982; Mithila et al. 2001).
Virus-infected leaves of Pelargonium zonale showed chlorotic rings, and flecks
as disease symptoms. Meristem tip culture is a promising method for the production
of virus-free plants. Meristem tips are grown on basal media with various combina-
tions of plant growth hormones, and the meristem responds differently in different
media. For example, meristem growth in basal media supplemented with
α-naphthalene acetic acid (NAA) and coconut milk results only in callus formation,
while supplementation of basal medium with low quantities of auxin, indole acetic
acid and kinetin results in plantlet formation. Plants regenerated from virus-free
meristem as explants are established as stocks of virus-free mother plants for future
usage (Hakkaart and Hartel 1979). Xanthomonas pelargonii-free P. x domesticum is
produced by this method (Cassells et al. 1987). Due to some drawbacks, such as low
survival rate of explants in initial stages and low numbers of shoot production, this
technique has limited applications at the industrial level (Desilets et al. 1993).
from the basal regions of cotyledons of young (2–4 day old) seedlings. The maxi-
mum shoot regeneration was observed when hypocotyls were regenerated on
IAA + zeatin or thidiazuron-supplemented media, while root explant regeneration
was suggested on zeatin-supplemented media. Croke and Cassells (1997) achieved
adventitious shoot regeneration from hypocotyl explants of P. x hortorum. The cau-
logenic potential of the root of Pelargonium x hortorum FI hybrids was determined
by growing root explants on MS media supplemented with tri-iodobenzoic acid
(TIBA) and thidiazuron (TDZ), and significant shoot regeneration was obtained
(Doyle et al. 1999).
Qureshi and Saxena (1992) reported the production of adventitious shoots and
somatic embryos directly by culturing the mature seeds of hybrid geranium
(Pelargonium x hortorum Bailey) on MS media supplemented with different plant
growth regulators (BAP, BAP + IAA, and thidiazuron).
12.7.3 S
omaclonal Variations: A Novel Source for Crop
Improvement
Generally, plants regenerated from tissue culture practices are identical to source
plants in morphology and genetic constitution, and these plants are termed soma-
clones because they are generated from the same group of somatic cells. Sometimes,
during the tissue culture process, genetic variations are generated in somaclones
which distinguish them from the original plant in morphology as well as in other
features also; such kinds of variations in somaclones are termed somaclonal varia-
tions. These variations are the sources of new traits which may be helpful for crop
improvement, and if these variations are genetically stable for many generations,
then these lines can be incorporated in plant breeding programmes (Krishna et al.
2016).
In geranium, many authors reported the occurrence of somaclonal variations,
which are different in morphology as well as in phytochemical compositions
(Dunbar and Stephens 1989; Gupta et al. 2001; Gupta et al. 2002; Kulkarni et al.
2012; Ravindra et al. 2004; Ravindra and Kulkarni 2015). During the screening of
calliclones of geranium, Saxena et al. (2008a) observed two morphotypes that dif-
fered in the dentation patterns of leaves: one morphotype was with high dentated
leaves (HDLs), and another was with low dentated round leaves (LDLs). HDL and
LDL were different in many agronomically important traits, such as flowering time,
plant height, canopy size and the number of branches and, the most important one,
essential oil composition. After greenhouse trials these morphotypes were grown
and observed for genetic stability (Saxena et al. 2008a, b). Two isomenthone-rich
344 L. K. Narnoliya et al.
(64.4% and 67.7%) somaclones were obtained during the propagation practice of
Pelargonium sp. (Kulkarni et al. 1998). Although, these variations in tissue culture
may be spontaneous, sometimes, they can be incorporated through some agents like
mutagens. In geranium, explants of ‘Bourbon’ and ‘Narmada’ cultivars were treated
with N-nitroso-N-methylurea (NMU) to obtain somaclonal variations showing bet-
ter oil yields (Kulkarni et al. 2014). By using somaclonal variation strategies,
disease-
resistance callus culture of Pelargonium graveolens cv. Hemanti was
obtained, which further led to the generation of plants resistant against Alternaria
alternata (Saxena et al. 2008a, b).
References
Arrigoni-Blank MD, Almeida SA, Oliveira AC, Blank AF (2011) Micropropagation and acclima-
tization of geranium (Pelargonium graveolens L.). Revista Brasileira de Plantas Medicinais
13(3):271–275
Babu KG, Kaul VK (2005) Variation in essential oil composition of rose scented geranium
(Pelargonium sp.) distilled by different distillation techniques. Flavour Fragr J 20(2):222–231
Bi YM, Cammue BP, Goodwin PH, KrishnaRaj S, Saxena PK (1999) Resistance to Botrytis cine-
rea in scented geranium transformed with a gene encoding the antimicrobial protein Ace-
AMP1. Plant Cell Rep 18(10):835–840
Bigos M, Wasiela M, Kalemba D, Sienkiewicz M (2012) Antimicrobial activity of geranium oil
against clinical strains of Staphylococcus aureus. Molecules 17(9):10276–10291
Boase MR, Bradley JM, Borst NK (1998) An improved method for transformation of regal pel-
argonium (Pelargonium X domesticum Dubonnet) by Agrobacterium tumefaciens. Plant Sci
139(1):59–69
12 The Phytochemical Composition, Biological Effects and Biotechnological… 347
Eisenreich W, Bacher A, Arigoni D, Rohdich F (2004) Biosynthesis of isoprenoids via the non-
mevalonate pathway. Cell Mol Life Sci 61(12):1401–1426
Elmann A, Mordechay S, Rindner M, Ravid U (2010) Anti-neuroinflammatory effects of geranium
oil in microglial cells. J Funct Foods 2(1):17–22
Finer JJ (1995) Direct somatic embryogenesis. In: Plant cell tissue organ cult. Springer, Berlin,
Heidelberg, pp 91–102
Friedrich H (1976) Phenylpropanoid constituents of essential oils. Lloydia 39(1):1–7
Ghannadi A, Bagherinejad MR, Abedi D, Jalali M, Absalan B, Sadeghi N (2012) Antibacterial
activity and composition of essential oils from Pelargonium graveolens L’Her and Vitex agnus-
castus L. Iran J Microbiol 4(4):171
Giongo JL, Vaucher R, Borin DI, Correa MS, Dos Santos VB, Santos RC, Boligon AA, Athayde
ML, Bonez PC, Rossi GG, De Campus MM (2015) Antimycobacterial, antimicrobial and anti-
fungal activities of geranium oil loaded nanocapsules. Int J Pharm Sci 7:414–419
Gupta P, Goel R, Pathak S, Srivastava A, Singh SP, Sangwan RS, Asif MH, Trivedi PK (2013) De
novo assembly, functional annotation and comparative analysis of Withania somnifera leaf and
root transcriptomes to identify putative genes involved in the withanolides biosynthesis. PLoS
One 8(5):e62714
Gupta R, Banerjee S, Mallavarapu GR, Sharma S, Khanuja SP, Shasany AK, Kumar S (2002)
Development of a superior somaclone of rose-scented geranium and a protocol for inducing
variants. Hort Sci 37(4):632–636
Gupta R, Mallavarapu GR, Banerjee S, Kumar S (2001) Characteristics of an isomenthone-rich
somaclonal mutant isolated in a geraniol-rich rose-scented geranium accession of Pelargonium
graveolens. Flavour Fragr J 16(5):319–324
Haensch KT (2004) Morpho-histological study of somatic embryo-like structures in hypocotyl
cultures of Pelargonium× hortorum bailey. Plant Cell Rep 22(6):376–381
Hakkaart FA, Hartel G (1979) Virus eradication from some Pelargonium zonale cultivars by
meristem-tip culture. Eur J Plant Pathol 85(2):39–46
Hamdorf G (1976) Propagation of Pelargonium varieties by stem-tip culture. Acta Hortic
59:143–152
Harney PM (1982) Tissue culture propagation of some herbaceous horticultural plants. In:
Application of plant cell and tissue culture to agriculture and industry. University of Guelph,
Ontario, pp 187–208
Hassanein A, Chevreau E, Dorion N (2005) Highly efficient transformation of zonal (Pelargonium
x hortorum) and scented (P. capitatum) geraniums via Agrobacterium tumefaciens using leaf
discs. Plant Sci 169(3):532–541
Hayakawa H, Sobue F, Motoyama K, Yoshimura T, Hemmi H (2017) Identification of enzymes
involved in the mevalonate pathway of Flavobacterium johnsoniae. Biochem Biophys Res
Commun 487(3):702–708
Helfer M, Koppensteiner H, Schneider M, Rebensburg S, Forcisi S, Müller C, Schmitt-Kopplin
P, Schindler M, Brack-Werner R (2014) The root extract of the medicinal plant Pelargonium
sidoides is a potent HIV-1 attachment inhibitor. PLoS One 9(1):e87487
Hutchinson MJ, KrishnaRaj S, Saxena PK (1997) Inhibitory effect of GA 3 on the development
of thidiazuron-induced somatic embryogenesis in geranium (Pelargonium xhortorum Bailey)
hypocotyl cultures. Plant Cell Rep 16(6):435–438
Jadaun JS, Sangwan NS, Narnoliya LK, Singh N, Bansal S, Mishra B, Sangwan RS (2017)
Over-expression of DXS gene enhances terpenoidal secondary metabolite accumulation in
rose-scented geranium and Withania somnifera: active involvement of plastid isoprenogenic
pathway in their biosynthesis. Physiol Plant 159(4):381–400
Khosravi AR, Shokri H, Sharifrohani M, Mousavi HE, Moosavi Z (2012) Evaluation of the anti-
fungal activity of Zataria multiflora, Geranium herbarium, and Eucalyptus camaldolensis
essential oils on Saprolegnia parasitica infected rainbow trout (Oncorhynchus mykiss) eggs.
Foodborne Pathog Dis 9(7):674–679
12 The Phytochemical Composition, Biological Effects and Biotechnological… 349
Narnoliya LK, Kaushal G, Singh SP, Sangwan RS (2017) De novo transcriptome analysis of rose-
scented geranium provides insights into the metabolic specificity of terpene and tartaric acid
biosynthesis. BMC Genomics 18(1):74
Narnoliya LK, Rajakani R, Sangwan NS, Gupta V, Sangwan RS (2014) Comparative transcripts
profiling of fruit mesocarp and endocarp relevant to secondary metabolism by suppression sub-
tractive hybridization in Azadirachta indica (neem). Mol Biol Rep 41(5):3147–3162
Narnoliya LK, Sangwan RS, Singh SP (2018a) Transcriptome mining and in silico structural and
functional analysis of ascorbic acid and tartaric acid biosynthesis pathway enzymes in rose-
scanted geranium. Mol Biol Rep 45(3):315–326
Narnoliya LK, Jadaun JS, Singh SP (2018b) Synthetic biology advances for enrichment of bio-
active molecules inplants. In: Recent trends and techniques in plant metabolic engineering.
Springer Nature, Singapore, pp 117–145. https://doi.org/10.1007/978-981-13-2251-8_6.
isbn:978-981-13-2251-8
Pellegrineschi A, Damon JP, Valtorta N, Paillard N, Tapfer D (1994) Improvement of ornamental
characters and fragrance production in lemon-scented geranium through genetic transforma-
tion by Agrobacterium rhizogenes. Nature Biotechnol 12(1):64–68
Putievsky E, Ravid U, Dudai N (1990) The effect of water stress on yield components and essential
oil of Pelargonium graveolens L. J Essent Oil Res 2(3):111–114
Qureshi JA, Saxena PK (1992) Adventitious shoot induction and somatic embryogenesis with
intact seedlings of several hybrid seed geranium (Pelargonium x hortorum Bailey) varieties.
Plant Cell Rep 11(9):443–448
Rajeswara Rao BR, Bhattacharya AK (1997) Yield and chemical composition of the essential oil
of rose-scented geranium (Pelargonium species) grown in the presence and absence of weeds.
Flavour Fragr J 12(3):201–204
Rajeswara Rao BR, Bhattacharya AK, Kaul PN, Chand S, Ramesh SI (1993) Changes in profiles
of essential oils of rose-scented geranium (Pelargonium sp.) during leaf ontogeny. J Essent Oil
Res 5(3):301–304
Rajeswara Rao BR, Sastry KP, Prakasa Rao EV, Ramesh SI (1990) Variation in yields and qual-
ity of geranium (Pelargonium graveolens L’Hér. ex Aiton) under varied climatic and fertility
conditions. J Essent Oil Res 2(2):73–79
Range CM, Rudolph EW, Ajay PS, Michael ER, Jonathan MF, James C, Charles RK (2011) Rare
exitary amino acid from flower of zonal geranium responsible for paralyzing the Japanese
beetle. PNAS 108(4):1217–1221
Rao BR (2009) Chemical composition and uses of Indian rose-scented Geranium (Pelargonium
species) essential oil-A review. J Essent Oil Bear Plant 12(4):381–394
Rastogi S, Meena S, Bhattacharya A, Ghosh S, Shukla RK, Sangwan NS, Lal RK, Gupta MM,
Lavania UC, Gupta V, Nagegowda DA (2014) De novo sequencing and comparative analysis
of holy and sweet basil transcriptomes. BMC Genomics 15(1):588
Ravindra NS, Kulkarni RN (2015) Essential oil yield and quality in rose-scented geranium: varia-
tion among clones and plant parts. Sci Hort 184:31–35
Reuther G (1982) Propagation of disease-free Pelargonium cultivars by tissue culture. Acta Hortic
131:311–320
Robichon MP, Renou JP, Jalouzot R (1995) Genetic transformation of Pelargonium X hortorum.
Plant Cell Rep 15(1):63–67
Rath CC, Dash SK, Rao BR (2005) Antifungal activity of rose-scented geranium (Pelargonium
species) essential oil and its six constituents. J Essent Oil Bear Plants 8(2):218–222
Ravindra NS, Kulkarni RN, Gayathri MC, Ramesh S (2004) Somaclonal variation for some mor-
phological traits, herbyield, essential oil content and essential oil composition in an Indian
cultivar of rose-scented geranium. Plant Breed 123(1):84–86
Sangwan NS, Farooqi AH, Shabih F, Sangwan RS (2001) Regulation of essential oil production in
plants. Plant Growth Regul 34(1):3–21
12 The Phytochemical Composition, Biological Effects and Biotechnological… 351
Sangwan RS, Tripathi S, Singh J, Narnoliya LK, Sangwan NS (2013) De novo sequencing and
assembly of Centella asiatica leaf transcriptome for mapping of structural, functional and reg-
ulatory genes with special reference to secondary metabolism. Gene 525(1):58–76
Saxena G, Banerjee S, Verma PC, Mallavarapu GR, Kumar S (2007) Rose-scented geranium
(Pelargonium sp.) generated by Agrobacterium rhizogenes mediated Ri-insertion for improved
essential oil quality. Plant Cell Tissue Organ Cult 90(2):215–223
Saxena G, Verma PC, Banerjee S, Kumar S (2008a) Field performance of somaclones of rose
scented geranium (Pelargonium graveolens L’Her Ex Ait.) for evaluation of their essential oil
yield and composition. Ind Crop Prod 27(1):86–90
Saxena G, Verma PC, Rahman LU, Banerjee S, Shukla RS, Kumar S (2008b) Selection of leaf
blight-resistant Pelargonium graveolens plants regenerated from callus resistant to a culture
filtrate of Alternaria alternata. Crop Prot 27(3):558–565
Sharopov FS, Zhang H, Setzer WN (2014) Composition of geranium (Pelargonium graveolens)
essential oil from Tajikistan. Am J Essent Oil Nat Prod 2:13–16
Silva NCC, Fernandes AJ (2010) Biological properties of medicinal plants: a review of their anti-
microbial activity. J Venom Anim Toxins Incl Trop Dis 16:402–413
Singh M, Singh UB, Ram M, Yadav A, Chanotiya CS (2013) Biomass yield, essential oil yield
and quality of geranium (Pelargonium graveolens L. Her.) as influenced by intercropping with
garlic (Allium sativum L.) under subtropical and temperate climate of India. Ind Crop Prod
46:234–237
Smith RH (2013) Plant tissue culture: techniques and experiments. Academic Press, Amsterdam
Sukhumpinij P, Kakihara F, Kato M (2010) In vitro regeneration from mature leaf explants of
Pelargonium rapaceum (L.) L’Hérit. Sci Hortic 126(3):385–389
Swanson JK, Yao J, Tans-Kersten J, Allen C (2005) Behavior of Ralstonia solanacearum race 3
biovar 2 during latent and active infection of geranium. Phytopathology 95(2):136–143
Singh P, Khan S, Kumar S, ur Rahman L (2017) Establishment of an efficient Agrobacterium-
mediated genetic transformation system in Pelargonium graveolens: an important aromatic
plant. Plant Cell Tissue Org Cult (PCTOC) 129(1):35–44
Slimmon T, Qureshi JA, Saxena PK (1991) Phenylacetic acid-induced somatic embryogenesis
in cultured hypocotylexplants of geranium (Pelargonium x hortorum Bailey). Plant Cell Rep
10(11):587–589
Theisen LL, Muller CP (2012) EPs® 7630 (Umckaloabo®), an extract from Pelargonium sidoides
roots, exerts anti-influenza virus activity in vitro and in vivo. Antivir Res 94(2):147–156
Tuleja M, Krupa A, Góralski G, Płachno BJ (2014) Morphological and histological events in the
preliminary tissue culture of haploid and diploid Pelargonium zonale var.‘Kleiner Liebling’.
Mod Phytomorphol 6:39–40
Van Zaayen A, Van Eijk C, Versluijs JM (1992) Production of high quality, healthy ornamental
crops through meristem culture. Acta Bot Neerl 41(4):425–433
Visser C, Qureshi JA, Gill R, Saxena PK (1992) Morphoregulatory role of thidiazuron substitution
of auxin and cytokinin requirement for the induction of somatic embryogenesis in geranium
hypocotyl cultures. Plant Physiol 99:1704–1707
Visser-Tenyenhuis C, Murthy BNS, Odumeru J, Saxena PK (1994) Modulation of somatic embryo-
genesis in hypocotyl-derived cultures of geranium (Pelargonium x hortorum Bailey) cv. Ringo
Rose by a bacterium. In Vitro Cell Dev Biol 30P:140–143
Verma RK, Chauhan A, Verma RS, Rahman LU, Bisht A (2013) Improving production poten-
tial and resources use efficiency of peppermint (Mentha piperita L.) intercropped with gera-
nium (Pelargonium graveolens L. Herit ex Ait) under different plant density. Ind Crop Prod
44:577–582
Verma RK, Verma RS, Rahman LU, Yadav A, Patra DD, Kalra A (2014) Utilization of distillation
waste–based vermicompost and other organic and inorganic fertilizers on improving produc-
tion potential in geranium and soil health. Commun Soil Sci Plant Anal 45(2):141–152
352 L. K. Narnoliya et al.
Aditya Banerjee and Aryadeep Roychoudhury
13.1 Introduction
Aromatic oils are extracted from plants by distillation processes like hydrodistilla-
tion (HD) and steam distillation (SD). Such oils consist of monoterpenes, sesquiter-
penes and their oxygenated derivatives like alcohols, aldehydes and ketones (Coelho
et al. 2012). Aromatic oils have great impacts in food, cosmetic, and pharmaceutical
industries (Table 13.1). However, such traditional techniques have several cons. The
thermolabile compounds often get degraded during extraction, thus altering the
crude flavour and aromatic profile of the compound. The isolated product thus can
be an altered derivative of the actual product. To overcome such limitations, the
solvating power of supercritical fluids have been utilised to design an alternative
technique called supercritical fluid extraction (SFE) (Fornari et al. 2012). The most
environmental-friendly supercritical fluid suitable for aromatic oil extraction from
plant tissues is carbon dioxide (CO2). Supercritical operations can be performed
with CO2 at relatively low pressures and room temperatures. The post-operational
extract is also solvent free and ready to be processed. However, this technique has a
high initial cost which often stands as a barrier during execution (Coelho et al.
2012). SFE-mediated volatile oil extraction has been successfully performed from
aromatic varieties like Mentha pulegium, Foeniculum vulgare, Coriandrum sati-
vum, Satureja fruticosa, Satureja montana, Santolina chamaecyparisus and Thymus
vulgaris (Coelho et al. 2012).
Plants have evolved some specialised cells forming glandular trichomes and osmo-
phores which secrete aromatic volatiles and oils. Non-specialised tissues like ducts
and cavities secrete terpenes. Figure 13.1 is a schematic representation of aromatic
oil production from such tissues.
GTs are epidermal hairs dedicated for secreting abundant quantities of secondary
products like mucilage, nectar, acyl lipids and essential oils (Lange and Turner
2013). The cocktail of organic chemicals secreted by GTs in aromatic plants has
immense demand in flavour and fragrance industries. The chemistry of the aromatic
exudates of GTs is of great interest as its unravelling can promote the potential uses
of the product (Lange and Turner 2013). Aromatic monocot members from
Tradescantia, Dioscorea and Sisyrinchium contain extensive epidermal GTs (Chwil
2011; Chauveau et al. 2011). The GTs are more prevalent in the dicot families like
13 Biotechnological Production of Aromatic Oils from Plants 355
Table 13.1 List of popular and important aromatic oils, their active principles, plant sources and
economic uses
Aromatic oil
(Active principles) Source Uses References
Agar oil Aquilaria Priced for perfume Korinek et al.
(4-phenyl-2-butanone, malaccensis (2016)
jinkoheremol, α-guaiene)
Ajwain oil Carum Has anti- Boskabady et al.
(35–65% thymol, carvacrol, copticum inflammatory, (2014)
terpinene, paracymene) antiaflatoxigenic and
pharmacological
effects
Anise oil Pimpinella Antimicrobial effects Gradinaru et al.
(licorice) anisum (2014)
Basil oil Ocimum Aromatherapy and Perumalsamy
(citral, α-terpeneol, linalool) basilicum antiseptic effects et al. (2014)
Black pepper oil Piper nigrum Treatment for muscle Bagheri et al.
(β-carophyllene, limonene, aches, sprains and (2014)
sabinene, 3-carene, α-pinene, indigestion
β-pinene)
Camphor oil Cinnamomum Treatment for cold, Fu et al. (2015)
(α-pinene, camphene, β-pinene, camphora cough and arthritis
sabinene, phellandrene, limonene,
1,8-cineole, γ-terpinene, p-cymene,
terpinolene, furfural, camphor,
linalool, bornyl acetate, terpinen-
4-ol, caryophyllene, borneol,
piperitone, geraniol, safrole,
cinnamaldehyde, methyl cinnamate,
eugenol)
Cardamom oil Amomum Used in cosmetics Joshi et al.
(1,8-cineole, α-terpineol, subulatum and as flavour (2013)
DL-limonene, nerolidol, 4-terpineol, enhancers
δ-terpineol, δ-3-carene, β-myrcene,
germacrene D, α-terpinene,
longifolenaldehyde)
Citronella oil Cymbopogon Insect repellent Zamora et al.
(geraniol, citronellol, limonene, winteratus (2015)
citronellal)
Coconut oil Cocos nucifera Used in foods and Gunasekaran
(essential fatty acids) cosmetics et al. (2017)
Clove oil Eugenia Relieves dental Taher et al.
(60–80% eugenol, acetyl eugenol, caryophyllata problems; acts as a (2015)
carophyllene) neuroprotective,
antinociceptive and
antipyretic agent
Davana oil Artemisia Used as a germicide Bhagavathy
(davanone and related oxygenated pallens et al. (2015)
sesquiterpenes)
(continued)
356 A. Banerjee and A. Roychoudhury
Table 13.1 (continued)
Aromatic oil
(Active principles) Source Uses References
Eucalyptus oil Eucalyptus spp. Insect repellent and Sebei et al.
(α-pinene, 1,8-cineol, contains medicinal (2015)
pinocarveol-trans) properties
Fennel seed oil Trachyspermum Treatment of colic Zheljazkov et al.
(trans-anethole, α-phellandrene, ammi (2013)
α-pinene, β-pinene)
Ginger oil Zingiber Treatment of nausea Sasidharan et al.
(geranyl acetate, zingiberene, officinale and cough (2012)
geranial)
Jasmine oil Jasminum Used in cosmetics Ye et al. (2015)
(benzyl acetate, linalool, cis- grandiflorum and therapeutics
jasmonate, geraniol, methyl
anthranilate)
Lemon oil Citrus limonum Used as an antiseptic Thany et al.
(α-pinene, camphene, sabinene, and in cosmetics (2015)
myrcene, α-terpinene, linalool,
β-bisabolene, limonene, trans-α-
bergamotene, nerol, neral)
Mustard oil Brassica spp. Used in cooking Wendlinger et al.
(allyl isothiocyanate and related purposes (2014)
derivatives)
Neem oil Azadirachta Multiple medicinal Scudeler et al.
(azadirachtin, nimbin, nimbidin, indica and antiseptic uses (2017)
nimbidol, sodium nimbinate,
gedunin, salannin, quercetin)
Orange oil Citrus sinensis Used as fragrance in Zhao et al.
(90% limonene) cleaning products (2015)
and flavouring foods
Oregano oil Origanum Fungicide Mohiti-Asli and
(thymol and carvacrol) vulgare Ghanaatparast-
Rashti (2015)
Perilla oil Perilla Improves Jang et al.
(50–60% perillaldehyde) frutescens cardiovascular (2014)
system, inhibits
platelet aggregation
and thrombus
formation
Rosemary oil Rosemarinus Used to soothe Raskovic et al.
(cineole, camphor, camphene, officinalis muscles and as an (2015)
α-pinene, diterpene lactone, anti-microbial agent
carnosol)
Sandalwood oil Santalum album Used in cosmetics Braun et al.
(α-santalol, β-santalol) (2014)
Turmeric oil Curcuma longa Used as a flavouring Jacob and
(curcumin) agent and has Badyal (2014)
anti-cancerous
properties
13 Biotechnological Production of Aromatic Oils from Plants 357
Fig. 13.1 The specialised and non-specialised anatomical sites of aromatic oil production in
plants
13.3.2 Osmophores
Osmophores also referred to as the floral fragrance glands are distributed on the
sepals and petals to allure potential pollinators. These glands are specialised in scent
emission and occur as conical-papillate cell clusters in the floral organ (Anton et al.
2012). Osmophores usually constitute of homogeneously layered glandular epithe-
lium with dense cytoplasm and starch deposition within the mesophyll. The cells of
358 A. Banerjee and A. Roychoudhury
the epidermis lack such deposits and this anatomical feature can be used to distin-
guish between the emission and production layers (Weryszko-Chmielewska and
Chwil 2010). Osmophores have been observed on the petal epidermis in Stanhopea,
Sievekingia, Galanthus nivalis and members belonging to Araceae and Orchidaceae
(Rehman et al. 2016).
The anatomical conical shape of osmophores has been accredited to the MIXTA
gene. Overexpression of this gene from Antirrhinum majus (Scrophulariaceae) in
tobacco (Solanaceae) resulted in the development of ectopically secreting trichomes
throughout the plant. This indicates a correlation between the osmophores and mat-
uration of secreting trichomes (Glover et al. 1998). Floral tissue-specific overex-
pression of this gene in targeted aromatic species can be performed to scale up the
production of industrially important aromatic oils. Production of fragrance is asso-
ciated with cytoplasmic lipid inclusions and amyloplastidic plastoglobuli, present in
plants like Sievekingia and Stanhopea. Due to the wrinkled surface of osmophores,
the remnants of secretion can be identified on the epidermal surface of Stanhopea
graveolens (Weryszko-Chmielewska and Chwil 2010).
13 Biotechnological Production of Aromatic Oils from Plants 359
In coniferous plants, ducts and cavities are used to excrete diterpenoid resin acids
dissolved in volatile turpentine. Upon pathogen infestation, the turpentine evapo-
rates leading to pathogen entrapment within the crystallised resin mass (Berton
2007). Several members belonging to families like Asteraceae, Apiaceae, Rutaceae,
Helianthaceae and Rubiaceae possess ducts and cavities to excrete out aromatic
exudates which have immense industrial uses (Bombo et al. 2012). Budel et al.
(2012) showed the presence of secretory ducts and non-GTs in the leaves and stems
of members of the Baccharis genus. Plants belonging to Brassicaceae release vola-
tile monoterpenoids and sesquiterpenoids from leaves after injury. Such emission is
mediated without the help of any specialised secretory tissue (Ahuja et al. 2010).
Oil bodies present in liverworts are single membrane encapsulated intracytoplasmic
secretory bodies which are derived from the dilation of the cisternae of the endo-
plastic reticulum (Marinho et al. 2014). Characterisation of candidate genes regulat-
ing such anatomical demarcations of oil production is yet to be performed.
As discussed, aromatic oils are of high industrial demand but are naturally produced
in minute amounts in the plant system. Several chemical protocols have been uti-
lised to refine the quality of oils for economic marketing. However, these proce-
dures have not been beneficial for boosting the inherent production of oils within
the system. Current advances in this field indicate that a biotechnological aptitude
involving metabolic engineering, transgenic approaches and enzymatic technolo-
gies can improve the production and refine natural quality of aromatic oils
(Fig. 13.3).
For enhancing the levels of S-linalool, the linalool synthase (LIS) gene from
Clarkia breweri was overexpressed in Lavandula latifolia (spike lavender). The lin-
alool content increased by 1000% in the youngest leaves of the transgenic plants
(Mendoza-Poudereux et al. 2014). The transgenic plants were cross-pollinated to
generate double transgenics expressing 1-deoxy-d-xylulose-5-P synthase (encodes
DXS, the first enzyme of methyl-d-erythritol-4-phosphate pathway) and LIS.
Interestingly, the double transgenics exhibited lower linalool content in the aromatic
oils than the parental lines possibly due to co-suppression effects associated with
the construct structure used (Mendoza-Poudereux et al. 2014). Overexpression of
DXS from Arabidopsis thaliana in spike lavender plants increased the aromatic oil
content by 101.5–359% when compared with the control plants (Muñoz-Bertomeu
et al. 2006). The transgenic plants showed no deterioration in chlorophyll and carot-
enoid contents, morphology, growth, development, flowering and seed germination
with respect to those of control plants devoid of the transgene. Thus, DXS
360 A. Banerjee and A. Roychoudhury
Fig. 13.3 Biotechnological increase of plant aromatic oil content is mediated by overexpressing
positive regulatory genes like LIS, TDC and DXS which encode crucial enzymes participating in
oil biosynthesis. Overexpression of LEC1 increases the seed oil content in canola by accelerating
fatty acid biosynthesis. The transcription factors like MYB and YABBY5 negatively regulate ter-
pene synthesis in spearmint plants. Hence the RNAi-mediated knock-in lines exhibited high accu-
mulation of terpenes
o verexpression greatly increased the aromatic oil content in lavenders without det-
rimentally affecting plant development and fitness (Muñoz-Bertomeu et al. 2006).
Mustard oil glycosides are derivatives of methionine, phenylalanine or trypto-
phan. Overexpression of tryptophan decarboxylase (TDC) in Brassica napus
(canola) plants reprogrammed the metabolic pathway leading to higher accumula-
tion of tryptamine instead of indole glucosinolates (Chavadej et al. 1994).
Interestingly, the indole glucosinolate content in the transgenic plants was only 3%
of that in the control plants, showing the efficient diversion of metabolites towards
biotechnologically created metabolic sinks (Chavadej et al. 1994). It is known that
the expression of fatty acid (FA) synthetic genes in Arabidopsis is enhanced by the
embryo developmental regulator, LEAFY COTYLEDON 1 (LEC1) (Banerjee and
Roychoudhury 2014). The canola seed oil content increased by 7–16% in the trans-
genic lines overexpressing BnLEC1. This was due to increased carbon flux towards
FA biosynthesis via alterations in the activities of enzymes involved in sucrose
metabolism, glycolysis and FA anabolism (Elahi et al. 2016).
13 Biotechnological Production of Aromatic Oils from Plants 361
Mentha spicata (spearmint) plants produce aromatic oils in peltate glandular tri-
chomes (PGTs). Reddy et al. (2017) recently identified a PGT-specific R2R3-MYB
gene, MsMYB, from comparative RNA-Seq data analysis in spearmint. The MsMYB-
RNAi knock-in lines exhibited elevated levels of monoterpenes, the precursors of
aromatic oil in spearmint. It was also observed that ectopic expression of MsMYB in
sweet basil and tobacco negatively regulated the sesquiterpene- and diterpene-
derived metabolite synthesis (Reddy et al. 2017). Though phylogenetic analyses
revealed that MsMYB could regulate the phenylpropanoid pathway, it was experi-
mentally revealed that this TF was more specific to the terpene biosynthetic path-
way (Reddy et al. 2017). Wang et al. (2016) functionally characterised a PGT-specific
novel MsYABBY5 gene in spearmint. The knock-in lines produced by MsYABB5-
RNAi led to higher accumulation of terpenes, showing that MsYABBY5 is a nega-
tive regulator of terpene biosynthesis (Wang et al. 2016). Such RNAi lines can be
developed on large scales to enhance the production of aromatic oils from spearmint
plants. Jin et al. (2014) performed next generation sequencing (NGS; Illumina
paired end sequencing) of spearmint RNAs from PGT, leaf and leaf stripped of
PGTs. The elaborate transcriptome analysis characterised the potential differen-
tially expressed genes in spearmint which can be targeted for metabolic engineering
for sustainable production of aromatic oils (Jin et al. 2014). Enzymes have also been
utilised in the production of aromatic oils. It was observed that cutinase-catalysed
hydrolysis of oil palm empty fruit bunch fibre (OPEFBF) lignin was more efficient
than the manganese peroxidase-induced oxidation. The level and quality of aro-
matic compounds were also conserved in the enzyme-treated product (Tang et al.
2015).
The concept of biotechnology and aromatic oil production is a new but rapidly
evolving field. Investigations which have been highlighted in this chapter clearly
indicate at the potential of metabolic engineering in boosting aromatic oil produc-
tion and quality at the industrial scale. The aromatic oils are produced in specialised
structures like the glandular trichomes, osmophores, ducts and cavities. These oils
usually are the derivatives of terpenes. This field encompasses several future per-
spectives. The genes regulating the anatomical niche of the specialised structures
can be targeted to enlarge the oil-producing units of the plants. The aromatic plants
can be converted into specialised oil-producing bio-factories by overexpressing
candidate rate-limiting enzyme-encoding genes and by downregulating the
TF-encoding genes which negatively regulate the oil-producing pathway. An
exhaustive analysis at the transcriptomic and metabolomic levels is quintessential
for identification of such candidate loci.
362 A. Banerjee and A. Roychoudhury
References
Jacob JN, Badyal DK (2014) Biological studies of turmeric oil, part 3: anti-inflammatory and
analgesic properties of turmeric oil and fish oil in comparison with aspirin. Nat Prod Commun
9:225–228
Jang JY, Kim TS, Cai J, Kim J, Kim Y et al (2014) Perilla oil improves blood flow through inhibi-
tion of platelet aggregation and thrombus formation. Lab Anim Res 30:21–27
Jin J, Panicker D, Wang Q, Kim MJ, Liu J et al (2014) Next generation sequencing unravels the
biosynthetic ability of spearmint (Mentha spicata) peltate glandular trichomes through com-
parative transcriptomics. BMC Plant Biol 14:292
Joshi R, Sharma P, Sharma V, Prasad R, Sud RK, Gulati A (2013) Analysis of the essential oil
of large cardamom (Amomum subulatum Roxb.) growing in different agro-climatic zones of
Himachal Pradesh, India. J Sci Food Agric 93:1303–1309
Korinek M, Wagh VD, Lo IW, Hsu YM, Hsu HY et al (2016) Antiallergic phorbol ester from the
seeds of Aquilaria malaccensis. Int J Mol Sci 17:398
Lange BM, Turner GW (2013) Terpenoid biosynthesis in trichomes--current status and future
opportunities. Plant Biotechnol J 11:2–22
Marinho CR, Souza CD, Barros TC, Teixeira SP (2014) Scent glands in legume flowers. Plant Biol
(Stuttg) 16:215–226
Mendoza-Poudereux I, Muñoz-Bertomeu J, Navarro A, Arrillaga I, Segura J (2014) Enhanced
levels of S-linalool by metabolic engineering of the terpenoid pathway in spike lavender leaves.
Metab Eng 23:136–144
Mohiti-Asli M, Ghanaatparast-Rashti M (2015) Dietary oregano essential oil alleviates experimen-
tally induced coccidiosis in broilers. Prev Vet Med 120:195–202
Muñoz-Bertomeu J, Arrillaga I, Ros R, Segura J (2006) Up-regulation of 1-deoxy-D-5-phosphate
synthase enhances production of essential oils in transgenic spike lavender. Plant Physiol
142:890–900
Perumalsamy H, Kim JY, Kim JR, Hwang KN, Ahn YJ (2014) Toxicity of basil oil constituents
and related compounds and the efficacy of spray formulations to Dermatophagoides farinae
(Acari: Pyroglyphidae). J Med Entomol 51:650–657
Raskovic A, Milanovic I, Pavlovic N, Milijasevic B, Ubavic M, Mikov M (2015) Analgesic effects
of rosemary essential oil and its interactions with codeine and paracetamol in mice. Eur Rev
Med Pharmacol Sci 19:165–172
Reddy VA, Wang Q, Dhar N, Kumar N, Venkatesh PN et al (2017) Spearmint R2R3-MYB tran-
scription factor MsMYB negatively regulates monoterpene production and suppresses the
expression of geranyl diphosphate synthase large subunit (MsGPPS.LSU). Plant Biotechnol
J 15:1105–1119
Rehman R, Hanif MA, Mushtaq Z, Mochona B, Qi X (2016) Biosynthetic factories of essential
oils: the aromatic plants. Nat Prod Chem Res 4:227
Sasidharan I, Venugopal VV, Menon AN (2012) Essential oil composition of two unique ginger
(Zingiber officinale Roscoe) cultivars from Sikkim. Nat Prod Res 26:1759–1764
Scudeler EL, Garcia AS, Pinheiro PF, Santos DC (2017) Neem oil (Azadirachta indica A. Juss)
affects the ultrastructure of the midgut muscle of Ceraeochrysa claveri (Navás, 1911)
(Neuroptera: Chrysopidae). Acta Histochem 119:84–91
Sebei K, Sakouhi F, Herchi W, Khouja ML, Boukhchina S (2015) Chemical composition and anti-
bacterial activities of seven Eucalyptus species essential oils leaves. Biol Res 48:7
Taher YA, Samud AM, El-Taher FE, ben-Hussin G, Elmezogi JS et al (2015) Experimental evalua-
tion of anti-inflammatory, antinociceptive and antipyretic activities of clove oil in mice. Libyan
J Med 10:28685
Tang PL, Hassan O, Maskat MY, Badri K (2015) Production of monomeric aromatic compounds
from oil palm empty fruit bunch fiber lignin by chemical and enzymatic methods. Biomed Res
Int 2015:891539
Thany SH, Tong F, Bloomquist JR (2015) Pre-treatment of Stegomyia aegypti mosquitoes with
a sublethal dose of imidacloprid impairs behavioural avoidance induced by lemon oil and
DEET. Med Vet Entomol 29:99–103
364 A. Banerjee and A. Roychoudhury
Yannis Gounaris
14.1 Introduction
The plant essential oils are mixtures of compounds, most of them characterized as
secondary metabolites, but in certain cases, they can be compounds resulting from
primary metabolism, such as fatty acids. The most common classes of secondary
metabolites in essential oils are terpenoids, followed by phenolics. A few of these
compounds predominate, whereas many more might be present in lesser amounts
in the oils, usually at 0.1–5%. Terpenoids comprise the largest and structurally
most varied class of secondary metabolites, numbering over 40,000 different mol-
ecules. Members of the 10-carbon group of terpenoids, the monoterpenoids, are
the main constituents of plant essential oils. These essential oils can also contain
sesquiterpenoids, phenylpropanoids, and benzenoids. In addition, plant tissues can
produce volatile aldehydes, their corresponding alcohols and acids, as well as vol-
atile ketones. These compounds can be occasionally found in essential oils, but
they are usually formed only in certain plant tissues and under specific physiologi-
cal conditions that favor catabolic reactions. They can be considered to belong
to the primary metabolites, although they can have useful flavoring or medicinal
applications.
The volatility of the essential oils reflects their composition. Volatility is deter-
mined mainly by the molecule’s ability to form hydrogen bonds. Of the terpenoids,
the only members of the mono- (C10) and sesquiterpenoid (C15) classes are suffi-
ciently volatile. Monoterpenoid molecules made of carbons and hydrogen only are
Y. Gounaris (*)
University of Thessaly, Department of Agriculture, Fytokou street,
New Ionia, GR38446, Greece
e-mail: [email protected]
very volatile. Those having one hydroxyl, keto, peroxy, or epoxy group are still
volatile, but those with more hydroxyls are either only slightly volatile or not vola-
tile at all. In sesquiterpenoids, the presence of one hydroxyl group is the maximum
level of tolerance for volatility. Triterpenoids and higher-order terpenoids are not
volatile. Phenylpropanoids and benzenoids with one hydroxyl and no carboxyl
group are volatile. The simultaneous presence of a keto group does not abolish these
molecules’ volatility. However, sporting more hydroxyl groups drastically reduces
or even completely abolishes their volatility. (Hydroxy)-cinnamic acids are not suf-
ficiently volatile, due to the presence of the carboxyl group, unless it is esterified
with a volatile alcohol. Aliphatic and olefinic aldehydes, monoalcohols, and mono-
ketones are volatile for at least up to 12-carbon sizes. As the molecule becomes
smaller than five carbons, even the acids become volatile. Organic monocarboxylic
acids, esterified with volatile alcohols, are also volatile.
There is intense commercial interest on essential oils because of their pharma-
ceutical properties. Moreover, essential oils have been also used in cosmetics, as
food additives and as flavoring compounds. Fresh plant seeds, flowers, stems, and
roots usually contain 0.1–10% v/w of essential oil, but this can range from <0.1%
up to 20% v/w in some cases—and even higher have also been known. Essential oils
are often produced from specific plant tissues, such as the seeds or flowers, whose
total mass from a single harvesting season is only a small percentage of the whole
weight of the plant. This is the reason why obtaining useful volatile essential oils
from cultivated plants can be so expensive. Chemical manufacture is often so much
cheaper that the natural products correspond only to a small percentage of the mar-
ket. However, consumers often prefer the natural product because they believe it to
be free of manufacturing artifacts and leftovers. Also, the chemical synthesis often
results in racemic mixtures of the product, resulting in only an approximation of the
natural flavor qualities. Thus, there is a need to reduce the cost of the natural
product.
Biotechnology attempts to facilitate the production and, therefore, to reduce the
market cost of essential oils. Some of the approaches attempt to produce them in
bioreactor facilities either by the action of cultured plant cell and tissues or by the
use of bacterial and fungal biotransformers. Semi-synthetic methods, in which a
precursor molecule is transformed into a useful product by isolated (crude or puri-
fied) enzyme preparations in vitro, have also been attempted. Along a different
approach, plants have been genetically engineered to overproduce the desired essen-
tial oil. Recently, such efforts have involved such metabolic engineering of the bio-
synthetic pathways leading to the synthesis of the desired essential oil compound.
We are accustomed of connecting essential oils with herbs. Yet, essential oils can
also be produced from trees, shrubs, and vines. Research on the biotechnology of
producing essential oils from such plants is not as intense as it is for herbaceous
species. It is the purpose of the present chapter to summarize the current progress in
biotechnological development for essential oil production from non-herbaceous
plants and, also, to suggest promising future directions of economically viable
approaches for “natural” essential oil production.
14 The Role of Biotechnology in Essential Oil Production from Non-herbaceous Plants 367
A list of the trees, shrubs, and vines known to produce useful essential oils is given
in Table 14.1. In addition to the references provided in Table 14.1, information on
their essential oil yield and composition, as well as on their cultivation methods and
uses, is provided by Guenther (1982) and Atal and Kapur (1982). This list is not
exhaustive as it is expected that more plant species will be added in the future, as
many other, so far unexamined, essential oils become known by ethnobotanists
exploring isolated human communities.
The extraction methods, plant parts used, and resulting essential oil yields can
vary widely. Wintergreen oil yield is 0.66% of the fresh weight of leaves and stems;
it contains 96–99% methyl salicylate and is used as a flavoring agent. Methyl salicy-
late is also the major component of birch oil, obtained by the distillation of chipped
birch bark that yields 950–1450 ml of oil per ton of wood. To produce labdanum
essential oil, twigs of the perennial shrub Cistus ladaniferus are boiled for some
hours, and the resin rising to the surface is collected and dried. The dry resin is then
steam-distilled to yield about 1–2% of essential oil. Direct distillation of fresh stems
is also possible, producing about 0.06% of essential oil. The oil pressure extracted
from coconut nuts is treated with superheated steam to obtain a volatile oil, still of
disagreeable odor and also containing several carbinols and ketones. Both oils can-
not be used as such in the perfume industry but can serve as starting materials for
the preparation of synthetic aromatics. Fir oil is produced at a yield of 0.65–1.4%,
by the distillation of young branches and leaves of Abies balsamea. By breaking the
vesicles beneath the bark, turpentine can be collected, which is then distilled to get
15–25% of essential oil. Distillation of pine needles for 5 h yields 0.25–0.35% of
pine oil, whose composition depends largely on the geographic origin of the Pinus
sylvestris. This oil is used in perfume preparations. The essential oil of Thuja pli-
cata, obtained by distillation, in addition to thujone, contains also the very poison-
ous γ-thujaplicin. The cypress oil is most valuable in the perfume industry.
Distillation for 17 h of young branches and leaves of Cupressus sempervirens pro-
duces about 0.2% of oil, yet the most valuable portion—the one having the amber-
gris and labdanum-like odor—is extracted after the first 3 h of distillation. Chips of
Juniperus virginiana heartwood yield on distillation 2–2.5% of cedarwood oil, con-
taining 80% of cedrene. However, cedrene and cedrol are found only in cut and aged
heartwood of Juniperus and not in live tissues of the plant. Juniperus communis
berries contain 0.5–2% of essential oil used for flavoring beverages and liquors. The
oil composition can change if fermentation precedes distillation.
It becomes clear from the above discussion that essential oil extraction needs
considerable expertise in order to be successful. One has to choose not only the right
plant location and tissues but also the appropriate extraction process and then secure
the removal of compounds of disagreeable odor or even poisonous, as well as pre-
vent the formation of unwanted artifacts. Biotechnology could potentially remove
some of the complications as well as increase the yield and facilitate the isolation of
essential oils or of their individual components.
368 Y. Gounaris
Table 14.1 (continued)
Main ingredients in order of
Plant Essential oil decreasing percentage References
15 Citrus aurantium Neroli oil Limonene, (E)-nerolidol, Ammar et al.
subsp. amara α-terpineol, α-terpinyl acetate, (E, (2012)
(bitter orange) E)-farnesol
16 Citrus bergamia Bergamot Limonene, linalyl acetate, linalool, Sawamura et al.
(bergamot orange) essential oil γ-terpinene, β-pinene (2006)
17 Citrus limon Lemon oil Limonene, γ-terpinene, β-pinene Gök et al. (2015)
(lemon tree)
18 Citrus medica Citron oil In leaves: erucylamide, limonene, Bhuiyan et al.
(citron) citral (2009)
In peel: isolimonene, citral,
limonene
19 Citrus reticulata Mandarin oil Limonene, γ-terpinene, α-Pinene Boughendjioua
(mandarin orange) and
Boughendjioua
(2017)
20 Citrus sinensis Orange oil Limonene, myrcene, sabinene Azar et al. (2011)
(orange tree)
21 Citrus tangerine Tangerine oil Limonene, γ-terpinene, myrcene, Njoroge et al.
(tangerine tree) α-pinene (2006)
22 Citrus paradisi Grapefruit oil Limonene, α-terpinene, α-pinene Njoroge et al.
(grapefruit) (2005)
23 Cocos nucifera Coconut oil Methyl laurate, methyl myristate da Fonseca et al.
(coconut tree) (2014)
24 Coffea arabica Coffee oil Dextrins, chlorogenic acid, caffeine Nogaim et al.
(coffee tree) (2013)
25 Commiphora Myrrh oil n-Octyl acetate, 4-ethynyl-4- Chen et al. (2013)
myrrha hydroxy-3,5,5-trimethyl
(myrrh) 2-cyclohexen-1-one, nerolidol
isobutyrate, β-elemene, copaene
26 Cupressus spp. Cypress oil α-Pinene, δ-3 carene, sabinene, Pierre-Leandri
(cypress) α-cadinol, terpinen-4-ol, limonene et al. (2003)
27 Eucalyptus Eucalyptus In leaves: 1,8-cineole, α-pinene, Chalchat et al.
globulus oil p-cymene, cryptone, spathulenol (1995)
(eucalyptus) In fruits: aromadendrene,
1,8-cineole
In buds: aromadendrene
1,8-cineole, α-thujene
In branches: 1,8-cineole,
aromadendrene
28 Illicium verum Star anise oil Trans-anethole Wei et al. (2014)
(star anise)
29 Juniperus Juniper berry α-Pinene, myrcene, sabinene, Höferl et al.
communis oil limonene, β-pinene (2014)
(Juniper)
(continued)
370 Y. Gounaris
Table 14.1 (continued)
Main ingredients in order of
Plant Essential oil decreasing percentage References
30 Juniperus Cedarwood Cedrene, α-Pinene, β-pinene, Stewart et al.
virginiana oil phelandrene (2014)
(red cedar)
31 Laurus nobilis Bay oil 1,8-Cineole, α-terpinyl acetate, Kivrak et al.
(bay) α-pinene, terpinen-4-ol, sabinene, (2017)
methyl eugenol, eugenol
32 Lawsonia inermis Henna oil Eugenol, ethyl hexadecanoate, Adebola et al.
(henna tree) (E)-methyl cinnamate, (2005)
isocaryophyllene, (E)-β-ionone,
methyl linolenate
33 Litsea cubeba Litsea cubeba Geranial, neral, D-limonene Si et al. (2012)
(may change) oil
34 Melaleuca Tea tree oil Leaves: Terpinen-4-ol, γ-terpinene, Carson et al. 2006
alternifolia α-terpinene, 1,8-cineole,
(tea tree) terpinolene
35 Moringa oleifera Moringa seed Oleic acid, palmitic acid, stearic Ghazali and
(drumstick tree) oil acid, behenic acid Abdulkarim
(2011)
36 Murraya koenigii Curry leaf oil Sabinene, α-pinene, phellandrene, Mallavarapu et al.
(curry tree) terpinen-4-ol, β-pinene, γ-terpinene (1999)
37 Myristica fragrans Nutmeg seed Sabinene, α-pinene, Ogunwande et al.
(nutmeg) oil α-phellandrene, terpinen-4-ol (2003)
38 Myroxylon Balsam of Benzyl benzoate Swift (1997)
balsamum (L.) Peru
(Myroxylon)
39 Picea spp. Spruce oil β-Pinene, camphor, α-pinene, Garneau et al.
(spruce) bornyl acetate (2012)
Bornyl acetate is the main
compound in Black Spruce
40 Pinus sylvestris Pine oil In branches: α-pinene, sabinene, Zafra and
(pine) β-pinene, limonene García-Peregrín
In needles: α-pinene, camphene, (1976)
β-pinene, sabinene
41 Ravensara Raversara oil Methyl chavicol, methyl eugenol, Andrianoelisoa
aromatica α-terpinene, limonene, sabinene, et al. (2006)
(clove nutmeg) linalool, terpinen-4-ol
42 Santalum Sandalwood α-Santalol, β-sandalol, Kusuma and
paniculatum or S. oil α-bergamotol, (E)-nuciferol, Mahfud (2016)
album germacrene B, teresantalol
(sandalwood)
43 Sassafras spp. Sassafras oil Safrole, camphor Sethi et al. (1976)
(sassafras) In root bark: safrole,
5-methoxyeugenol, asaron,
piperonylacrolein, coniferaldehyde,
camphor
(continued)
14 The Role of Biotechnology in Essential Oil Production from Non-herbaceous Plants 371
Table 14.1 (continued)
Main ingredients in order of
Plant Essential oil decreasing percentage References
44 Thuja plicata Western red α-Thujone (cis), sabinene, Tsiri et al. (2009)
(western red cedar oil β-thujone (trans), terpinen-4-ol
cedar)
45 Tsuga canadensis Tsuga oil α-Pinene, camphene, limonene Kılıç and Kocak
(tsuga, Canadian (2014)
hemlock)
Shrubs
46 Cistus ladaniferus Labdanum α-Pinene, trans-pinocarveol, Mariotti et al.
(labdanum) viridiflorol, ledol (1997)
47 Gaultheria Wintergreen Methyl salicylate, limonene Nikolić et al.
procumbens oil (2013)
(American
wintergreen)
48 Jasminum Jasmine oil In flowers: We et al. (2015)
officinale 3,7,11,15- tetramethyl-2-
(jasmine) hexadecen-1-ol(phytol),
3,7,11- trimethyldodeca -1,6,10-trien-
3-ol, 3,7,11,15-
tetramethyl −1-Hexadecen-3-ol
49 Myrtus communis Myrtle oil α-Pinene, 1,8-cincole, α-limonène, Mulas and Melis
(myrtle) linalool, myrtenyl acetate (2011)
50 Pelargonium Geranium oil Citronellol (48.44%), α- pinene, Mousavi et al.
graveolens octen-1-o1, geraniol, p-menthone, (2014)
(rose geranium) β-caryophyllene
51 Rhododendron Ledum oil In leaves and shoots: γ-terpineol Gretšušnikova
tomentosum (syn. In stem: β-myrcene et al. (2010)
Ledum palustre)
(wild rosemary)
52 Rosa damascena Rose oil Citronelol, geraniol, nonadecane, Dobreva et al.
or Rosa centifolia nerol (2013)
(rose)
53 Rosa rubiginosa Rose hip oil Vitispiran, α-E-acaridial, Nowak (2005)
or Rosa mosqueta dodecanoic acid, hexadecanoic
(rose) acid, docosane (C22), β-ionone,
6-methyl-5-hepten-2-one,
2-heptanone, heptanal, myristic
acid and linolic acid
54 Vaccinium Cranberry Palmitic acid, linoleic acid, oleic Bhagdeo (2004)
oxycoccos seed oil acid, linolenic acid
(cranberry)
Vines
55 Piper nigrum Black pepper β-Caryophyllene, α-copaene, Rmili et al.
(black pepper) oil sabinene, cubenol (2014)
56 Schisandra Schisandra oil Ylangene, β-himachalene Chen et al. (2011)
chinensis α-bergamotene, β-chamigrene
(magnolia-vine)
372 Y. Gounaris
In order to genetically manipulate the essential oil yield and composition, the thor-
ough understanding of their biosynthesis is required. The monoterpenoids are pro-
duced by the plastidic methyl–erythritol–phosphate (MEP) path, whose sequence
and enzymatic properties have been explored (Estevez et al. 2001; Gao et al. 2006;
Julsing et al. 2007; Proteau 2004; Rohdich et al. 2000; Rohdich et al. 2001; Rohdich
et al. 2003; Wolfertz et al. 2004). The rate-limiting step is catalyzed by 1-deoxy-D-
xylulose 5-phosphate synthase (DXS), an enzyme consuming NADPH, CTP, and
ATP. The condensation of the produced dimethylallyl diphosphate (DMADP) by
use of isopentenyl diphosphate (IDP), in order to form the monoterpenoid precursor
geranyl diphosphate (GDP), is catalyzed by geranyl diphosphate synthase, a slow
catalyst. Subsequent cyclization of GDP in addition to slow cyclases, which are
membrane-bound enzymes in the plastids and endoplasmic reticulum, leads to the
synthesis of various monoterpenes. The hydroxylations of the linear or cyclic mono-
terpenes are catalyzed by NADPH-consuming, cyt450-dependent monooxygen-
ases, utilizing molecular oxygen or hydrogen peroxide. These hydroxylases are
inducible by a variety of biotic or abiotic stress factors. Sesquiterpenoids are con-
sidered to be synthesized in cytosol from farnesyl diphosphate (FDP), derived from
the mevalonic acid pathway. Two NADPH and three ATP molecules are consumed
for the FDP synthesis, and the rate-limiting step is catalyzed by the 3-hydroxymeth-
ylglutaryl coenzyme A reductase (HMGR). Sesquiterpene cyclases act on FDP to
produce at least 200 types of cyclic sesquiterpenoids.
The phenylpropanoid constituents of essential oils are produced from phenylala-
nine and tyrosine of the shikimic acid pathway (Goodwin and Mercer 1972;
Weisshaar and Jenkins 1998). The pathway is tightly regulated by feedback inhibi-
tion at several of its steps as well as by the need for NADPH and ATP up to the
synthesis of tyrosine and phenylalanine, with the requirement for NADPH being
even greater in the transformation of Phe and Tyr into phenylpropanoids. NADPH is
required for the reductive elimination of the carboxyl group of the propenyl side by
successive reductions that form the corresponding volatile aldehydes, alcohols, and
phenylpropenes. NADPH is also required for the hydroxylations of the aromatic
ring. Benzoic and phenolic acids come from the corresponding hydroxycinnamic
acids by β-oxidation of the propenyl chain, followed by oxidative decarboxylation.
This process is tightly regulated by feedback inhibition. Volatile derivatives are then
formed by the reduction of the carboxyl group, as in phenylpropanoids.
Non-branched volatile aldehydes and their corresponding alcohols can be
derived by degradation of unsaturated fatty acids, mainly linoleic and linolenic acid
(Combet et al. 2006; Feussner and Wasternack 2002) by the sequential action of
lipoxygenases (LOX), hydroperoxide lyases (HPL), and aldehydes dehydrogenase
(ADH). The initial introduction of molecular oxygen into the carbon–carbon double
bonds also requires NADPH. Methyl-branched volatiles are produced by the
catabolism of lineal terpenoids and of leucine-derived 3-methyl-crotonyl-CoA, via
degradation of the produced 3-methyl glutaryl-CoA (Hoschle et al. 2005). Methyl-
branched compounds, such as isovaleric and isobutyric acid, are also derived from
14 The Role of Biotechnology in Essential Oil Production from Non-herbaceous Plants 373
Callus or cell culturing has been achieved for almost all of the plants presented in
Table 14.1. However, most of these efforts lead to the genetic transformation of the
plant by focusing on genes not directly involved in secondary metabolism. There
was no reason to analyze the effect of in vitro culturing on the yield and composi-
tion of the essential oils in the untransformed or genetically transformed plants.
Table 14.2 presents the cases in which the essential oil or the production of a spe-
cific secondary metabolite was analyzed.
A comparison with Table 14.1 shows that the secondary metabolite profile of
in vitro cultured cells and calli is different from that of intact plants. As expected,
taking into account the results of many previous studies with various plant species,
the amounts were much reduced and, in some cases, no secondary metabolites were
detected at all. Application of ethylene-producing agents or pathogen elicitors stim-
ulates secondary metabolite synthesis. The exceptions are the alkaloids—caffeine
and trigonelline—that are synthesized at amounts greater and comparable (respec-
tively), compared to those in the intact plant tissues. Also the phenolic schisandrin
A was produced at half the yield recorded from plant tissues.
Plants used as sources of secondary metabolites are often rare or slow growing
or inhabiting difficult-to-approach localities and could be difficult to cultivate as
well. Tissue or cell culturing overcomes most of these problems. Unfortunately,
in vitro cultures are poor producers of secondary metabolites (Scragg 1995). The
ability of cultured plant tissue and cells to produce secondary metabolites is induc-
ible by a variety of chemical and physical factors (Olivoto et al. 2017; Shanker and
Shanker 2016). Cases of higher production from in vitro cultures, compared to
intact plant tissue, are also known. For example, Ocimum sanctum calli have less
total phenolics but more total terpenoids than intact plant tissues (Mathew and
Sankari 2014), although the monoterpene and sesquiterpene content has not been
separately examined. Callus cultures of Genista species produce more isoflavones,
374 Y. Gounaris
Table 14.2 Secondary metabolites produced by in vitro cultured tissues or cells of non-herbaceous
plant species
Plant Result/product Reference
Trees
Aquilaria Some essential oil was produced by callus or cell Jayaraman and
malaccensis suspension cultures. More fatty acid derivatives formed by Mohamed
(agarwood) elicitation with Trichoderma (2015)
Azadirachta 380 mg·L−1 azadirachtin in the cell suspension culture Srivastava and
indica medium Prakash (2010)
(neem)
Betula spp. Betulinic acid 2.01 g·kg−1 dry weight in callus. Betulin in Hajati et al.
(birch) cell suspension cultures was 0.96 g·kg−1 dry weight. Betulin (2016)
forms 30% of intact birch bark dry weight
Cananga Calli from petal explants. Only linalool and benzyl acetate Lindain et al.
odorata were detected. Less linalool and more benzyl acetate than in (2008)
(cananga tree) intact flowers. More linalool under light conditions and
more benzyl acetate in the dark
Citrus C. aurantifolia callus yielded limonene only, 4.4 mg·kg−1 Reila and
aurantifolia fresh weight. Grapefruit peel has 2500 mg·kg−1 Bergerh (1996)
(Lime)
Citrus aurantium No essential oil compound was detected in calli by Kriaa Kriaa (2012);
subsp. amara (2012), but del Rio et al. (1991) detected valencene at del Rio et al.
(bitter orange) 0.060 mg·kg−1 and nootkatone at 0.16 mg·kg−1 callus fresh (1991)
weight. The corresponding values in fruit pericarp are 3 and
20 mg·kg−1, respectively
Citrus Limon Valencene accumulated at 0.09 mg·kg−1 of callus fresh del Rio et al.
(lemon tree) weight. The corresponding values in fruit pericarp are 47 (1991)
and 131 mg·kg−1
Citrus sinensis Embryogenic calli yielded 5.4 μg·kg−1 essential oil of which Niedz et al.
(orange tree) 3.6 μg was 3-hydroxy-2-butanone (1997)
Citrus paradisi Valencene accumulated at 0.080 mg·kg−1 and nootkatone at del Rio et al.
(grapefruit) 1.6 mg·kg−1 callus fresh weight. The corresponding values (1991)
in fruit pericarp are 47 and 123 mg·kg−1, respectively
Coffea arabica 50- to 90-fold more caffeine in calli (4.5–10 kg per kg of Waller et al.
(coffee tree) callus) comparing to explant, calculated on a tissue dry (1983)
weight basis. Most of the caffeine was extracellular
Calli yielded intracellularly 2 g caffeine and 12 g Baumann and
chlorogenic acid per kg of callus dry weight. Explants have Rohrig (1989)
90 mg caffeine per kg dry weight
Cupressus spp. Cupressus lusitanica suspension cells accumulated 22 mg of Itose and Sakai
(cypress) β-thujaplicin per g dry cell weight in 3 days after elicitation (1997)
by insufficient inorganic nutrients and excess Fe
Monoterpenes and lignin were produced by Cupressus de Alwis et al.
lusitanica suspension cells induced by fungal elicitor or (2009)
mechanical stress
Illicium verum Calli produce anethole and foeniculin Kohda et al.
(star anise) (1997)
Juniperus Suspension cultures provided with phenylalanine Kašparová
virginiana synthesized podophyllotoxin (0.56 g·kg−1 tissue dry weight), et al. (2017)
(red cedar) 20 times less than in the intact plant tissues
(continued)
14 The Role of Biotechnology in Essential Oil Production from Non-herbaceous Plants 375
Table 14.2 (continued)
Plant Result/product Reference
Laurus nobilis Calli produce 1,8-cineole and α-pinene Rady and
(bay) Youssef (1999)
Lawsonia Lawsone (0.13% dry weight) was observed in hairy root Bakkali et al.
inermis tissues incubated in the dark (1997)
(henna tree)
Melaleuca Total polyphenolics in calli were 13 μg (as gallic acid Jeyakani
alternifolia equivalents) per g of fresh callus tissue Santhosh and
(tea tree) Rajalakshmi
(2016)
Moringa oleifera Callus cultures had 2.38 g kg−1 trigonelline, comparing to Mathur and
(drumstick tree) that in pods (3.55 g·kg−1), leaves (2.60 g·kg−1), roots Kamal (2012)
(2.15 g·kg−1), stem (1.90 g·kg−1), and flowers (1.60 g·kg−1)
Myristica β-Pinene, myristicin, safrole, methyl eugenol, and Indira-Iyer
fragrans betasitosterol were detected in embryogenic callus et al. (2009)
(nutmeg)
Santalum Various sesquiterpenes, including beta-santalol and Crovadore
paniculatum or betasantalene, were synthesized by S. album calli treated et al. (2012)
S. album with 1-aminocyclopropane-1-carboxylic acid (ACC)
(sandalwood) No essential oils, but phenolics are produced by stressed Valluri (2009)
sandalwood cell cultures
Santalols (5.2 mg·L−1) and phenolics (31 mg·L−1) are Misra and Dey
produced by somatic embryos (2013)
Thuja plicata Elicitation of calli by yeast extract results in accumulation Haluk and
(western red of tropolones in about 6.5 g·kg−1 cell fresh weight. In a Roussel-
cedar) medium supplemented with glucose, tropolone accumulates Bousta (2003)
to 8.1 g·kg−1 with elicitation and 3.15 g·kg−1 without
elicitation
Shrubs
Jasminum Jasminum officinale callus accumulated traces of several Banthorpe
officinale monoterpenes (<0.1% the amount in petals) et al. (1986)
(jasmine)
Pelargonium 0.54% oil yield by cell suspensions Aly and
graveolens Hanafy (2008)
(rose geranium)
Rosa damascena Only traces, if at all, of some essential oil components in Banthorpe and
(rose) callus or cell cultures of R. damascena Barrow (1983)
Vines
Piper nigrum Piperine 0.852 g·kg−1 dry weight of callus. Plantlets had Ahmad et al.
(black pepper) 4.16 g·kg−1 dry weight (2013)
Schisandra Lignans at 244.8 g·kg−1 accumulated in the calli. Szopa et al.
chinensis The lignans were not detected in the media (2016)
(magnolia-vine) Schisandrin A at 0.251 g·kg−1, 0.118 g·kg−1, and Zhou et al.
0.115 g·kg−1 was found in seeds, callus, and suspension (2017)
cells, respectively. Schisandrin B was 0.142 g·kg−1,
0.086 mg·kg−1, and 0.05 mg·kg−1, respectively
Schisandrin 67.70 g, deoxyschisandrin 55.19 g, gomisin A Szopa and
36.97 g, chlorogenic acid 15.33 g, and protocatechuic Ekiert (2016)
acid 13.11 g accumulated per kg dry weight of callus,
under blue light
376 Y. Gounaris
whereas callus and cell cultures of Maclura pomifera produce more flavones and
flavanones than the parent herbs (Filová 2014). Isoflavones and all flavonoids are
synthesized from phenylalanine in the cytoplasm (Winkel-Shirley 2001). From
Table 14.2, it can be seen that caffeine in Coffea arabica calli is higher than in
explants.
The reasons for the reduced ability of the in vitro culture to produce volatiles,
and secondary metabolites in general, are not known with certainty. The cultured
cells and callus seem to have some enzymatic activity for terpenoid production
(Banthorpe and Barrow 1983; Banthorpe et al. 1986; Soler et al. 1992; Zito et al.
1991). In the synthesis of phenylpropanoid, the enzymatic activities of phenylala-
nine ammonia lyase, shikimate dehydrogenase, cinnamic acid-4-hydroxylase,
p-coumaric acid-3 hydroxylase, cinnamoyl-CoA reductase, 4-coumarate:CoA
ligase, 4 hydroxycinnamate:CoA ligase, cinnamyl alcohol dehydrogenase, and caf-
feic acid O-methyltransferase in callus or cell suspensions have been found to often
be equal to those of intact plant tissues (Ali et al. 2006; Anterola et al. 2002;
Karyagina et al. 2007; Möller et al. 2006; Seidel et al. 2002).
An often-cited observation is that some degree of differentiation is required for
secondary metabolite synthesis by cell and callus cultures. For example, fine hair-
like structures (hairy roots) arise from plant tissues transformed with the transfer
DNA (T-DNA) regions of the Ri plasmid of Agrobacterium rhizogene. Their culture
methods, their morphological and biochemical characteristics, as well as their sec-
ondary metabolite production potential are all known (Figueiredo et al. 2006;
Georgiev et al. 2007; Srivastava and Srivastava 2007). Hairy roots lack geotropism,
and they are highly branched and can be cultured in bioreactor facilities requiring
no plant growth regulators, since the inserted T-DNA carries genes for auxin synthe-
sis. They grow as fast, or faster, than normal roots, with meristem cell cycles averag-
ing 10 h. They produce secondary metabolites at levels and patterns similar to those
of normal roots but also metabolites produced in aerial parts of the plant. Often
novel compounds are also produced. Unlike cell or callus cultures, hairy roots are
biochemically stable, and the T-DNA is reliably integrated.
The induction of essential oil production upon elicitation of cultured cells sug-
gests that the transcription level for the necessary enzymes is lower than in the intact
plant. In Croton stellatopilosus, for example, 1-deoxy-D-xylulose-5-phosphate syn-
thase (DXS), 2C-methyl-D-erythritol 4-phosphate synthase (MEPS), and geranyl-
geranyl diphosphate synthase (GGPPS) were highly expressed in the cells from
leaves and of the green callus culture but not in suspension cells, and this was in
agreement with the terpenoid profiling (Kongduang et al. 2014). DXS is not the
only regulatory step in plastidic terpenoid synthesis. The 1-deoxy-D-xylulose-5-
phosphate reductoisomerase (DXR) and 1-hydroxy-2-methyl-2-butenyl
4-diphosphate reductase (HDR) are also regulatory enzymes. Besides, back-
inhibition by the product DMADP regulates DXR. To enhance essential oil produc-
tion by calli and cell suspensions, it would perhaps be worthy to attempt simultaneous
transformation of plastids by all genes for the above three enzymes.
However, even the best results of inducing secondary metabolite synthesis by
genetically transformed callus or cell cultures cannot reach the levels of these com-
pounds in intact tissues. It seems that the availability of the necessary biosynthetic
14 The Role of Biotechnology in Essential Oil Production from Non-herbaceous Plants 377
enzymes is not the only limiting factor. It must be considered that the synthesis of
monoterpenoids, at least, is primarily carried out in the plastids and requires a lot of
NADPH. Auxins used in tissue culture inactivate the chloroplast photosynthetic
apparatus (Buggeln and Bal 1977; Volfová et al. 1978; Zubo et al. 2011).
Photosynthesis is also inactivated in these cases by the dark culturing regime used.
Under these conditions, plastids will have to rely on the oxaloacetate/malate shuttle
(Anderson and House 1979) for obtaining NADPH from the cytoplasm. The pub-
lished literature did not provide with any report on the concentration of NADPH or
on the functionality of the oxaloacetate/malate shuttle in callus plastids. We cannot
expect plastids, even with over-expressed regulatory enzymes of the MEP pathway,
to reach the levels of terpenoid synthesis of intact photosynthesizing plant tissues, if
the availability of intra-plastidic reducing equivalents is, indeed, the limiting factor.
The cases of genetic engineering efforts to alter the essential oil production and
constituency of non-herbaceous plants are very limited (Table 14.3). Hairy roots of
neem tree have been made to produce 7 mg of the furanolactone tertanor-triterpenoid
(limonoid) azadirachtin per kg of weight, but azadirachtin is primarily produced in
the plant’s seed kernels at amounts of 4–6 g·kg−1 (Mordue and Nisbet 2000). This
compound is not the prevalent one in the corresponding essential oil, but it is the one
endowing it with its pesticidal properties. Henna hairy roots produce the 2-hydroxy-
1,4-naphtho-quinone lawsone at amounts of 0.13% of dry weight of tissue, whereas
natural plant leaves contain these at 0.5–2% (Sabra et al. 2015). Two of several
regenerated rose geranium plants, also carrying the Ri plasmid, produced even more
geraniol and its esters. Since this was not a general characteristic of the majority of
regenerated transgenics, it is not known if it is due to the plasmid or to a variation
generated during the callus culturing and regeneration stage.
In black pepper, the isopentenyl pyrophosphate transferase gene reduced the
total monoterpenoids in favor of sesquiterpenoids, as expected by the shunting of
isopentenyl pyrophosphate to larger terpenoid synthesis. Again as expected, the
transgene for limonene synthase determined the levels of limonene and other mono-
terpenes in eucalyptus and orange oil. Both calli and hairy roots synthesize it at
levels higher than the plant roots but still at 1000-fold lower amounts than in the
seed kernels (Mordue and Nisbet 2000). Finally, transformation with sense or anti-
sense theobromine synthase and xanthosine-N7-methyltransferase affected the lev-
els of caffeine and theobromine in coffee plants.
Despite the small number of cases in which the essential oil or some of its com-
ponents were examined, transgenic trees, shrubs, and vines, such plant species
genetically transformed with a variety of genes exist, and it should not be difficult
to analyze the effects of genetic transformation on oil composition. For example,
transgenic birch with downregulated 4-coumaroyl-CoA-ligase gene has been cre-
ated, and its lignin, but not the essential oil, has been examined (Shestibratov et al.
2011). Similarly, only the lignin was examined in birch (Zhang et al. 2015) and
378 Y. Gounaris
c onnection between abiotic or biotic stress and secondary metabolite synthesis has
also been well documented, yet the examination of the effect on the essential oil was
not included in the researchers’ immediate interests at the time.
14.6 B
iotransformations of Essential Oils and Their
Constituents
Essential oils are very rarely used in their entirety in biotransformations. Although
the reasons for this are more empirical than researched, essential oils are usually
inhibitory to bacterial or fungal cell growth. Some cases of the use of the entire
essential oils in bioconversions are presented in Table 14.4. The general tendency is
for the essential oil components to be transformed into more oxygenated products
of the same or lower number of carbon atoms. For example, citron oil, consisting
primarily of the aliphatic monoterpenoids limonene, terpinene, and isolimonene
and citral, produced five-carbon valerolactones, octanol, the nine-carbon ketone
cryptone, and the ten-carbon alcohols hydroxycitronelol and cuminol. The 12-carbon
γ-dodecalactone was also produced. This valuable flavoring compound is a product
of fatty acid catabolism (An et al. 2013). Similarly, hydroxylated monoterpenoids
were produced from biotransformed pinenes of spruce turpentines. Interestingly,
the non-oxygenated monoterpenes of mandarin oil were transformed into triterpe-
noids by the fungus Antrodia cinnamomea, indicating that anabolic processes could
also utilize monoterpenes as substrates.
By far, the most successful biotechnology approaches are those using microor-
ganisms to bioconvert isolated compounds of essential oils. An extensive list has
been reported before (Gounaris 2010). However, Table 14.5 provides a more focused
and updated list for the major essential oil components from non-herbaceous plants.
The bioconversions can be achieved by bacterial, fungal, or plant cell suspensions,
or by isolated enzymes. Almost all transformations are catabolic oxygenations
Table 14.4 Cases of use of the entire essential oil from non-herbaceous plants in biotransformation
studies
Origin of the
essential oil Result Reference
Citrus medica Peel oil was transformed by Enterobacter agglomerans into Park et al.
(citron) trans-2-decenal, octanol, δ-valerolactone, γ-valerolactone, (2004)
cryptone, hydroxycitronellol, cuminol, and γ-dodecalactone
Citrus 4% peel extract was transformed by the mushroom Antrodia Ma et al.
reticulata cinnamomea into triterpenoids (2014)
(mandarin
orange)
Picea spp. P. abies turpentines (primarily pinenes) were transformed by Dvořaková
(spruce) suspension cultures of P. abies primarily into pinocarveol and et al. (2011)
myrtenol
Table 14.5 Bioconversions of non-herbaceous plant essential oil constituents
380
(2017b).
(continued)
381
Table 14.5 (continued)
382
2-phenylethanol
Usual substrate % in potential
Substrate Products Transforming organism Reference plant source*
Conversion to cinnamic acid Streptomyces viridosporus aldehyde Wiklof et al.
oxidase (bacterial enzyme) (1984)
Conversion to 3-phenylpropanol and Euglena gracilis (algae) Noma et al.
cinnamyl alcohol (1991)
Reduction to hydrocinnamyl alcohol Cell suspension culture of Shams-Ardakani
Glycyrrhiza glabra et al. (2005)
100% conversion to cinnamyl alcohol Yeast alcohol dehydrogenase (fungal Zucca et al.
enzyme) (2009)
Conversion (reduction) to cinnamyl Mucor sp. (mold, fungus) Ma et al. (2011)
alcohol
Citral Converted to thymol (21.5%), geranial Penicillium sp. (fungus) Esmaeili and Citron peel oil (23%); lime oil
(18.6%), and nerol (13.7%) Tavassoli (2010) (4%)
Primarily citronellol (48.5%) Saccharomyces cerevisiae (yeast, Esmaeili et al.
fungus) (2012)
1 mM citronellal Zymomonas mobilis and Citrobacter Müller et al.
freundii (anaerobic bacteria) (2006)
80% conversion to geraniol and nerol Cell suspensions of Vitis vinifera Ambid et al.
(1982)
Citronellol Cyclization to 25–26 g·l−1 rose oxide Penicillium sp. (fungus) Maróstica Jr and Rosa damascena oil (up to 48%)
Pastore (2006);
Pimentel et al.
(2012)
Oxidativelly halogenated by 51% to Chloroperoxidase from Piantini et al.
6-bromo-3,7-dimethyloctane-1,7-diol Caldariomyces fumago (fungal (2011)
enzyme)
Cyclization by 75% to rose oxide and by Aspergillus niger (fungus) Demyttenaere
concomitant oxidation to 12% nerol et al. (2004)
oxide
14 The Role of Biotechnology in Essential Oil Production from Non-herbaceous Plants
8- and 10-hydroxy citronellol in 13.0 and Suspension cells of Catharanthus Hamada et al.
50.0% yield, respectively roseus (2001)
383
(continued)
Table 14.5 (continued)
384
(continued)
385
Table 14.5 (continued)
386
Verbenone as the major product and Stereum hirsutum (fungus) Lee et al.
myrtenol, camphor, and isopinocarveol as (2015b) oil (up to 17%); nutmeg seed oil
minor products (13%); spruce oil (12%)
387
(continued)
Table 14.5 (continued)
388
14.7 Conclusions
The usefulness and utilization of tree, shrub, and vine essential oils in biotechno-
logical applications have only received limited exploration. In vitro cultured calli or
cell suspensions produce greatly reduced amounts of essential oil components, even
in the cases of genetically transformed tissues, carrying genes for terpenoid synthe-
sis. The exception is the synthesis of alkaloids. This is an observation generally
applicable to herbaceous species as well. The problem has to be tackled from a
novel approach, since the availability of the necessary biosynthetic enzymes does
not seem to be the limiting factor. Production of commercially useful compounds by
the biotransformation of individual essential oil components by bacteria, fungi, or
isolated enzymes is much more promising. The yields very often exceed the thresh-
old for a commercially useful application of 1 g of product/L of culture medium.
And the results are even more impressive in the case of microorganisms carrying
transgenes used for the biosynthesis of essential oil ingredients.
References
Ab Rahman SFBT (2009) Analysis of agarwood oil composition by thin layer chromatography.
Dissertation, Universiti Malaysia Pahang
Abraham W-R, Ernst L, Arfmann H-A (1990a) Rearranged caryophyllenes by biotransformation
with Chaetonium cochliodes. Phytochemistry 29(3):757–763
Abraham W-R, Ernst L, Stumpf B (1990b) Biotransformation of caryophyllene by Diplodia gos-
sypina. Phytochemistry 29(1):115–120
Adebola O, Ekundayo OO, Koenig WA (2005) Essential oil composition of Lawsonia inermis L.
leaves from Nigeria. J Essent Oil Res 17:403–404
Adeboye PT, Olsson L, Bettiga M (2016) A coniferyl aldehyde dehydrogenase gene from
Pseudomonas sp. strain HR199 enhances the conversion of coniferyl aldehyde by
Saccharomyces cerevisiae. Bioresour Technol 212:11–19
Aguilar JA, Zavala AN, Diaz-Perez C, Cervantes C, Diaz-Perez AL, Campos-Garcia J (2006) The
atu and liu clusters are involved in the catabolic pathways for acyclic monoterpenes and leucine
in Pseudomonas aeruginosa. Appl Environ Microbiol 72(3):2070–2079
390 Y. Gounaris
Ahmad N, Abbasi BH, ur Rahman I, Faza H (2013) Piper nigrum: micropropagation, antioxidative
enzyme activities, and chromatographic fingerprint analysis for quality control. Appl Biochem
Biotechnol 169:2004–2015
Al Harrasi A, Al Saidi S (2008) Phytochemical analysis of the essential oil from botanically certi-
fied oleogum resin of Boswellia sacra (Omani Luban). Molecules 13:2181–2189
Alaoui MI, Benjilali B (1994) Biotransformation of terpenic compounds by fungi. II-Metabolism
of α-(−)-thujone. Nat Prod Lett 4:263–266
Ali MB, Singh N, Shohael AM, Hahn E-J, Paek K-J (2006) Phenolics metabolism and lignin
synthesis in root suspension cultures of Panax ginseng in response to copper stress. Plant Sci
171(1):147–154
Allan E, Eeswara J, Jarvis A, Mordue A, Morgan E, Stuchbury T (2002) Induction of hairy root
cultures of Azadirachta indica A. Juss. and their production of azadirachtin and other important
insect bioactive metabolites. Plant Cell Rep 21:374–379
Aly U, Hanafy M (2008) Geranium oil production in suspension cultures of Pelargonium graveo-
lens L. Med Aromat Plant Sci Biotechnol 2:24–28
Ambid C, Moisseeff M, Fallot J (1982) Biogenesis of monoterpenes bioconversion of citral by a
cell suspension culture of Muscat grapes. Plant Cell Rep 1:91–93
Ammar AH, Bouajila J, Lebrihi A, Mathieu F, Romdhane M, Zagrouba F (2012) Chemical com-
position and in vitro antimicrobial and antioxidant activities of Citrus aurantium l. flowers
essential oil (Neroli oil). Pak J Biol Sci 15:1034–1040
An J-U, Joo Y-C, Oh D-K (2013) New biotransformation process for production of the fragrant
compound γ-dodecalactone from 10-hydroxystearate by permeabilized Waltomyces lipofer
cells. Appl Environ Microbiol 79(8):2636–2641
Anderson JW, House CM (1979) Polarographic study of dicarboxylic-acid-dependent export of
reducing equivalents from illuminated chloroplasts. Plant Physiol 64:1064–1069
Andrianoelisoa HS, Chantal Menut C, de Chatelperron PC, Saracco J, Ramanoelina P, Danthu
P (2006) Intraspecific chemical variability and highlighting of chemotypes of leaf essen-
tial oils from Ravensara aromatica Sonnerat, a tree endemic to Madagascar. Flavour Frag
J 21:833–838
Anterola AM, Jeon JH, Davin LB, Lewis NG (2002) Transcriptional control of monolignol biosyn-
thesis in Pinus taeda: factors affecting monolignol ratios and carbon allocation in phenylpro-
panoid metabolism. J Biol Chem 277(21):18272–18280
Ashengroph M (2017a) Salinivibrio costicola GL6, a novel isolated strain for biotransformation of
caffeine to theobromine under hypersaline conditions. Curr Microbiol 74:34–41
Ashengroph M (2017b) A novel strain of Aureobasidium sp. TeO12 for theophylline production
from caffeine. 3 Biotech 7:176. https://doi.org/10.1007/s13205-017-0793-7
Atal CK, Kapur BM (1982) Cultivation and utilization of medical plants. Regional Research
Laboratory, New Dehli
Azar PA, Nekoei M, Larijani K, Bahraminasab S (2011) Chemical composition of the essential oils
of Citrus sinensis cv.Valencia and a quantitative structure–retention relationshipstudy for the
prediction of retention indices by multiple linear regression. J Serb Chem Soc 76:1627–1637
Bakkali AT, Jaziri M, Foriers A, Vander Heyden Y, Vanhaelen M, Homes J (1997) Lawsone accu-
mulation in normal and transformed cultures of henna, Lawsonia inermis. Plant Cell Tissue
Organ Cult 51:83–87
Banthorpe DV, Barrow SE (1983) Monoterpene biosynthesis in extracts from cultures of Rosa
damascena. Phytochemistry 22:2127–2728
Banthorpe DV, Branch SA, Njar VCO, Osborne MG, Watson DG (1986) Ability of plant callus
cultures to synthesize and accumulate lower terpenoids. Phytochemistry 25(3):629–636
Başer KHC, Demirci B (2007) Studies on Betula essential oils. Arkivoc vii:335–348
Baumann TW, Rohrig L (1989) Formation and intracellular accumulation of caffeine and chloro-
genic acid in suspension cultures of Coffea arabica. Phytochemistry 28:2667–2669
Bhagdeo Μ (2004) Determination of extracTables from cranberry seeds using supercritical CO2.
Dissertation, Virginia Polytechnic Institute and State University
Bhuiyan MNI, Begum J, Sardar PK, Rahman MS (2009) Constituents of peel and leaf essential oils
of Citrus Medica L. J Sci Res 1:387–392
14 The Role of Biotechnology in Essential Oil Production from Non-herbaceous Plants 391
Li Y-Q, Kong D-X, Wu H (2013) Analysis and evaluation of essential oil components of cinnamon
barks using GC–MS and FTIR spectroscopy. Ind Crop Prod 41:269–278
Lindain AF, Reglos RA, de Guzman CC, Cedo MLO (2008) Tissue culture and essential oil pro-
duction from callus cultures of Ilang-Ilang [Cananga odorata (Lamk) Hook.f. & Thomson].
Philipp Agric Sci 91:251–260
Liu X, Xu J, Xia J, Lv J, Wu Z, Deng Y (2016) Improved production of citric acid by Yarrowia
lipolytica using oleic acid as the oxygen-vector and co-substrate. Eng Life Sci 16:424–431
Ma L, Liu X, Liang J, Zhang Z (2011) Biotransformations of cinnamaldehyde, cinnamic acid and
acetophenone with Mucor. World J Microbiol Biotechnol 27:2133–2137
Ma T-W, Lai Y, Yang F-C (2014) Enhanced production of triterpenoid in submerged cultures
of Antrodia cinnamomea with the addition of citrus peel extract. Bioprocess Biosyst Eng
37:2251–2261
Ma Y, Wang X, Xueling Nie X, Zhang Z, Yang Z, Nie C, Tang H (2016) Microbial degradation
of chlorogenic acid by a Sphingomonas sp. strain. Appl Biochem Biotechnol 179:1381–1392
Mallavarapu GR, Ramesh S, Syamasundar KV, Chandrasekhara RS (1999) Composition of Indian
curry leaf oil. J Essent Oil Res 11:176–178
Mariotti JP, Tomi F, Casanova J, Costa J, Bernardini AF (1997) Composition of the essential oil of
Cistus ladaniferus L. cultivated in Corsica (France). Flavour Frag J 12:147–151
Maróstica MR Jr, Pastore GM (2006) Biotransformation of citronellol in rose-oxide using cassava
wastewater as a medium. Ciencia Tecnol Alime 26(3):690–696
Mathew R, Sankari PD (2014) Comparison of major secondary metabolites quantified in elicited
cell cultures, non-elicited cell cultures, callus cultures and field grown plants of Ocimum. Int
J Pharm Pharm Sci 6(2):102–106
Mathur M, Kamal R (2012) Studies on trigonelline from Moringa oleifera and its in vitro regula-
tion by feeding precursor in cell cultures. Braz J Pharmacog 22:994–1001
Mi J, Schewe H, Buchhaupt M, Holtmann D, Schrader J (2016) Efficient hydroxylation of
1,8-
cineole with monoterpenoid resistant recombinant Pseudomonas putida GS1. World
J Microbiol Biotechnol 32:112
Mirata M-A, Wiist M, Mosandl A, Sell D, Schrader J (2006) Lilac aldehydes and lilac alcohols as
metabolic byproducts of fungal linalool biotransformation. In: Bredie WLP, Petersen MA (eds)
Flavour science: recent advances and trends. Elsevier, Netherlands
Misra BB, Dey S (2013) Culture of East Indian sandalwood tree somatic embryos in air-lift biore-
actors for production of santalols, phenolics and arabinogalactan proteins. AoB Plants 5:plt025.
https://doi.org/10.1093/aobpla/plt025
Miyazawa M, Miyasato Y (2001) Biotransformation of (+)- and (−)-bornyl acetate using the plant
parasitic fungus Glomerella cingulataas a biocatalyst. J Chem Technol Biotechnol 76:220–224
Miyazawa M, Takahashi T, Sakata K, Horibe I (2008) Biotransformation of three aromadendrane-
type sesquiterpenoids by Aspergillus wentii. J Chem Technol Biotechnol 83:1006–1011
Miyazawa M, Uemura T, Kameoka H (1995) Biotrnaformation of sesquiterpenoids, (+)-aroma-
dendrene and (−)-alloaromadendrene by Glomerella cingulata. Phytochemistry 40(3):793–796
Molina G, Bution ML, Bicas JL, Dolder MAH, Pastore GM (2015) Comparative study of the
bioconversion process using R-(+)- and S-(−)-limonene as substrates for Fusarium oxysporum
152B. Food Chem 174:606–613
Möller R, Ball RD, Henderson AR, Modze G, Find J (2006) Effect of light and activated charcoal
on tracheary element differentiation in callus cultures of Pinus Radiata D. Don. Plant Cell Tiss
Org Cult 85(2):161–171
Monzotea L, Hillb GM, Cuellarc A, Scullc R, Setzerb WN (2012) Chemical Composition and anti-
proliferative properties of Bursera graveolens essential oil. Nat Prod Commun 7:1531–1534
Mordue AJ, Nisbet AJ (2000) Azadirachtin from the neem tree Azadirachta indica: its action
against insects. An Soc Entomol Brasil 29(4):615–632
Mousavi ES, Dehghanzadeh H, Abdali A (2014) Chemical composition and essential oils of
Pelargonium graveolens (Geraniaceae) by gas chromatography – mass spectrometry (GC/MS).
Bull Env Pharmacol Life Sci 3:182–184
Mulas M, Melis RAM (2011) Essential oil composition of myrtle (Myrtus communis) leaves.
J Herbs Spices Med Plants 17:21–34
396 Y. Gounaris
Müller A, Hauer B, Rosche B (2006) Enzymatic reduction of the α,β-unsaturated carbon bond in
citral. J Mol Catal B Enzym 38:126–130
Nakahashi H, Miyazawa M (2011) Biotransformation of (−)-camphor by Salmonella typhimurium
OY1002/2A6 expressing human CYP2A and NADPH-P450 reductase. J Oleo Sci
60(10):545–548
Nanjundaiah S, Mutturi S, Bhatt P (2017) Modeling of caffeine degradation kinetics during culti-
vation of Fusarium solani using sucrose as co-substrate. Biochem Eng J 125:73–80
Nano GM, Binello A, Cravotto G, Bianco MA (2005) Biotransformation of (−)-bornyl acetate
using submerged cultures of Collybia velutipes, Trametes hirsuta and Ganoderma applanatum.
J Chem Technol Biotechnol 80:657–661
Niedz RP, Moshonas MG, Peterson B, Shapiro JP, Shaw PE (1997) Analysis of sweet orange
(Citrus sinensis (L.) Osbeck) callus cultures for volatile compounds by gas chromatography
with mass selective detector. Plant Cell Tissue Organ Cult 51:181–185
Nikolić M, Marković T, Mojović M, Pejind B, Savić A, Perić T, Marković D, Stević T, Soković M
(2013) Chemical composition and biological activity of Gaultheria procumbens L. essential
oil. Ind Crop Prod 49:561–567
Nishio T, Patel A, Wang Y, Lau PCK (2001) Biotransformations catalyzed by cloned p-cymene
monooxygenase from Pseudomonas putida F1. Appl Microbiol Biotechnol 55:321–325
Njoroge SM, Mungai HN, Koaze H, NTL P, Sawamura M (2006) Volatile constituents of mandarin
(Citrus reticulata Blanco) peel oil from Burundi. J Essent Oil Res 18:659–662
Njoroge SM, Koaze H, Karanja PN, Sawamura M (2005) Volatile constituents of redblush grape-
fruit (Citrus paradisi) and pummelo (Citrus grandis) peel essential oils from Kenya. J Agric
Food Chem 53:9790–9794
Nogaim QA, Al-Duais M, Al-Warafi A, Al-Erianee H, Al-Sayadi M (2013) The chemical composi-
tion of Yemeni green coffee. J Food Chem Nutr 1:42–48
Noma Y, Okajima Y, Takahashi H, Asakawa Y (1991) Biotransformation of aromatic aldehydes
and related compounds by Euglena gracilis Z. Phytochemistry 30(9):2969–2972
Nowak R (2005) Chemical composition of hips essential oils of some Rosa L. species. Z
Naturforsch 60c:369–378
Oda S, Fujinuma K, Inoue A, Ohashi S (2011) Synthesis of (−)-β-caryophyllene oxide via regio-
and stereoselective endocyclic epoxidation of β-caryophyllene with Nemania aenea SF
10099-1 in a liquid–liquid interface bioreactor (L–L IBR). J Biosci Bioeng 112(6):561–565
Oda S, Kato A, Matsudomi M, Ohta H (1996) Enantioselective oxidation of racemic citronellol
with an interface bioreactor. Biosci Biotech Biochem 60(1):83–87
Ogita S, Uefuji H, Morimoto M, Sano H (2004) Application of RNAi to confirm theobromine as
the major intermediate for caffeine biosynthesis in coffee plants with potential for construction
of decaffeinated varieties. Plant Mol Biol 54:931–941
Ogita S, Uefuji H, Yamaguchi Y, Koizumi N, Sano H (2003) RNA interference: producing decaf-
feinated coffee plants. Nature 423:823
Ogunwande IA, Olawore NO, Adeleke KA, Ekundayo O (2003) Chemical composition of essen-
tial oil of Myristica Fragrans Houtt (Nutmeg) from Nigeria. J Essent Oil Res 6:21–26
Ohara K, Matsunaga E, Nanto K, Yamamoto K, Sasaki K, Ebinuma H, Yazaki K (2010)
Monoterpene engineering in a woody plant Eucalyptus camaldulensis using a limonene syn-
thase cDNA. Plant Biotechnol J 8:28–37
Olivoto T, Maicon Nardino M, Carvalho IR, Follmann DN, Szareski VJ, Ferrari M, de Pelegrin
AJ, de Souza VQ (2017) Plant secondary metabolites and its dynamical systems of induction
in response to environmental factors: A review. Afr J Agric Res 12(2):71–84
Onken J, Berger RG (1999) Biotransformation of citronellol by the basidiomycete Cystoderma
carcharias in an aerated-membrane bioreactor. Appl Microbiol Biotechnol 51:158–163
Park J-Y, Lee S-H, Kim K-R, Park J-B, Oh D-K (2015) Production of 13S-hydroxy-9(Z)-
octadecenoic acid from linoleic acid by whole recombinant cells expressing linoleate
13-hydratase from Lactobacillus acidophilus. J Biotechnol 208:1–10
Park YJ, Baek HH, Bang O-K, Chang HC (2004) Conversion of citron (Citrus junos) peel oil by
Enterobacter agglomerans. J Microbiol Biotechnol 14(6):1275–1279
14 The Role of Biotechnology in Essential Oil Production from Non-herbaceous Plants 397
Willrodt C, Halan B, Karthaus L, Rehdorf J, Julsing MK, Buehler K, Schmid A (2017) Continuous
multistep synthesis of perillic acid from limonene by catalytic biofilms under segmented flow.
Biotechnol Bioeng 114(2):281–290
Winkel-Shirley B (2001) Flavonoid Biosynthesis. A colorful model for genetics, biochemistry, cell
biology, and biotechnology. Plant Physiol 126:485–493
Wolfertz M, Sharkey TD, Boland W, Kuhnemann F (2004) Rapid Regulation of the methylerythri-
tol 4-phosphate pathway during isoprene synthesis. Plant Physiol 135(4):1939–1945
Yang B, Gao H, Stantond C, Rosse RP, Zhang H, Chen YQ, Chen H, Chen W (2017) Bacterial
conjugated linoleic acid production and their applications. Prog Lipid Res 68:26–36
Zafra M, García-Peregrín E (1976) Seasonal variations in the composition of Pinus halepensis and
Pinus sylvestristwigs and needles essential oil. J Agric Sci 86:1–6
Zeng X-F, Zhao D-G (2016) Expression of IPT in Asakura-sanshoo (Zanthoxylum piperitum (L.)
DC. f. inerme Makino) alters tree architecture, delays leaf senescence, and changes leaf essential
oil composition. Plant Mol Biol Rep 34:649–658
Zhang W, Wei R, Chen S, Jiang J, Li H, Huang H, Yang G, Wang S, Wei H, Liu G (2015) Functional
characterization of CCR in birch (Betula platyphylla × Betula pendula) through overexpression
and suppression analysis. Physiol Plantarum 154:283–296
Zheng Y, Xu X, Zou X (2016) Biotransformation of caffeine in oolong tea by Paecilomyces gunnii.
Int Biodeterior Biodegradation 114:141–144
Zhou Y-Q, Li T-C, Cheng Y-P (2017) Production of schisandrin a and schisandrin b from callus
and suspension cell cultures of schisandra chinensis. Nat Prod Commun 12(6):937–940
Zito SW, Srivastava V, Adebayo-Olojo E (1991) Incorporation of [1-14 C]-isopentenyl pyro-
phosphate into monoterpenes by a cell-free homogenate prepared from callus cultures of
Chrysanthemum cinerariaefolium. Planta Med 57(5):425–427
Zubo YO, Yamburenko MV, Kusnetsov VV, Börner T (2011) Methyl jasmonate, gibberellic acid,
and auxin affect transcription and transcript accumulation of chloroplast genes in barley.
J Plant Physiol 168:1335–1344
Zucca P, Littarru M, Rescigno A, Sanjust E (2014) Cofactor recycling for selective enzymatic
biotransformation of cinnamaldehyde to cinnamyl alcohol. Biosci Biotechnol Biochem
73(5):1224–1226
Chapter 15
Eremothecium Oil Biotechnology
as a Novel Technology for the Modern
Essential Oil Production
15.1 Introduction
Essential oils are more significant products of plants, with sources ranging from
fungi and algae to flowering plants. There are around 3000 varieties of plants
existing in the world, which can be used as a source of essential oil. However,
nowadays, only about 200 of them are used for essential oil production; the majority
is plants of higher taxa. The quality of essential oil depends considerably on
ecological factors such as location, where volatile-oil-bearing plants are cultivated.
In addition, plantation cultivation is seasonal. Production using biotechnology is
free from these disadvantages. However, the biotechnology of essential oil
production using cultures of isolated cells and tissues is not as effective as the
biotechnology based on the microbial synthesis.
Certain bacteria, yeasts, actinomycetes, fungi, and algae can synthesize essential
oils and aromatic substances de novo and also bioconvert less valuable substances
(fatty acids, alcohols, alkanes, etc.) into essential oils. These organisms are of
particular interest as nontraditional sources in connection with the rapid development
of modern industrial biotechnology. In nature, there are around 100,000 known
species of microorganisms, but only a few hundred species synthesize products or
provide reactions which are beneficial to mankind (Быков et al. 2003).
E. F. Semenova (*)
Penza State University, Penza, Russia
E. V. Presnyakova
State Commission of the Russian Federation for Selection Achievements Test and Protection,
Moscow, Russia
A. I. Shpichka · V. S. Presnyakova
Institute for Regenerative Medicine, Sechenov University, Moscow, Russia
15.1.1 B
iotechnological Raw Material as a Nontrivial Source
of Fragrant Substances
Specific features of oil-bearing plants and their complex and various essential oil
compositions depend not only on their taxonomic status but also on the region they
are cultivated, harvest time and method, drying procedure, and storage conditions.
New species suitable for the production of essential oils were ascertained using
comprehensive research methodologies, which are as follows:
–– Search for new organisms using screening studies
–– Study of essential oil accumulation dynamics in ontogeny
–– Cultivation of introduced species and recommended development for rational
use of raw materials
–– Biotechnological study on production of biomass and bioactive substances
–– Complex application of producers and raw materials using resource-saving
technologies
–– Development of test parameters required for reference documentation
–– Improvement of applied methods
During introduction, new phytotechnologies and biotechnologies are developed
and realized in industries at specialized enterprises (Pandal 2006). To date, search
for new sources of essential oils and more raw materials is made possible using
microorganisms and in vitro cultures. The latter permits manufacturers to achieve
new forms with beneficial features, high phytomass yield, micropropagation, and
plant sanitation (Шпичка and Семенова 2016a).
Intensification of phyto- and biotechnologies for bioactive substance production
needs the fundamental and applied studies in the field of life sciences, including
genomics, proteomics, metabolomics, and nanobiotechnology. This also includes
the development of novel life system and product technologies aimed at increasing
the time and quality of life and supporting reproductive and labor potential in all
countries. Industrial introduction of new scientific achievements ensures techno-
logical, economic, and social development (Родов et al. 1987; Семенова and
Богданов 2000). Nowadays, together with the increase in production capacities
and expansion of cultured plant variety, we should take measures to improve the
raw material quality, including ecological cleanness, high yield achievement, sup-
port of biological balance, nature protection, rational use of raw materials, and so
on. The raw material base expansion for oil-bearing plants is possible due to their
cultivation under controlled conditions (Dudareva et al. 2013). Recently, such tech-
nologies as industrial hydroponics, biotechnology, and greenhouse farming have
become common.
For a long time, plants were the only source of volatile fragrant substances.
However, issues related to their cultivation and shortage in raw materials limit the
development of perfume, cosmetic, pharmaceutical, and food industries. Moreover,
15 Eremothecium Oil Biotechnology as a Novel Technology for the Modern… 403
the quality of these essential oils may vary under extreme conditions. Therefore, the
search for new sources of volatile fragrant substances has become crucial, and bio-
technological approaches are of high interest (Krings, and Berger 1998; Bicas et al.
2010). Particularly, microorganisms, which can produce such substances de novo or
convert less valuable substrates, are proven to be promising (Soetaert and Vandamme
2010). To date, several companies produce 4-decalactone with peach scent (BASF),
macrocyclic musk components (Nippon Mining Co., Quest International), vanillin
(Evolva), and so on (Janssens et al. 1992; Bomgardner 2012).
All studied bacteria species can be divided into four groups depending on differ-
ing scents of the synthesized substances. The first group is the rod bacteria which
belong to phyla Firmicutes and Actinobacteria and produces pyrazines with nutty
scent. The second group includes Gram-positive cocci and bacilli (Firmicutes,
Lactobacillales), which can synthesize creamy-scented ketones, ethers, aldehyde,
and simple acyloins. The third group consists of various microorganisms which
produce alcohols and esters with fruity scent (Table 15.1). The fourth group consists
of streptomyces synthesizing geosmin with earthy smell.
For fragrant compound industrial scaling, microorganisms from the second group
are of particular interest because these substances define organoleptic properties of
dairy products. Therefore, their extraction may decrease the end product quality.
However, revealing the pathways and mechanisms of creamy-scented compound
synthesis and the conditions influencing their cultivation is crucial for the dairy
industry (Longo and Sanroman 2006). The fruity odor of bacteria from the third
group usually consists of 2–5 components, which can be easily produced via
chemical synthesis in high yields (Janssens et al. 1992).
Microalgae are a promising source to produce fragrant products. The comparative
analysis of cyanobacteria and green and red algae, belonging to Calothrix,
Cylindrospermum, Anabaena, Nostoc, Spirulina, Chlorella, and Cyanidium, showed
that the quantity of the synthesized volatile fragrant compounds may reach up to
3 mg L−1 of cultural liquid (Погорельская et al. 1999; Семенова and Бугорский
1989). The biomasses from Chlorella vulgaris, Spirulina platensis, and others may
be used to prepare ethanol oakmoss resinoid-like extracts for coloring and odor fixa-
tion in perfumes (Table 15.2) (Mitishev at al. 2016).
The most promising microorganisms for essential oil and fragrant compound
production are fungi. They can accumulate interesting and bioactive metabolites in
large quantities (Tables 15.2 and 15.3). Moreover, micromycetes can synthesize
more complex fruity-scented compounds (lactones) than bacteria (Vandamme
2003). Despite their chemical synthesis, lactones produced by fungi are optically
active and easily accessible. For instance, Trichoderma viride generates strong
coconut odor during its cultivation on simple media. This odor is mostly caused by
the 6-pentyl-2-pyrone synthesis, and its quantity reaches 170 mg L−1 of cultural
liquid. To produce this compound via chemical synthesis, at least seven steps are
required. Peach-like scent can be obtained using Sporobolomyces odorus cultures
that synthesize 4-decalactone (Haeusler and Muench 1998; Hansen et al. 2009;
Bicas et al. 2010).
Basidiomycetes and other fungi produce volatile fragrant substances with mush-
room odor. It is caused by aliphatic eight-carbon compounds (e.g., 1-octen-ol,
1-octen-3on, 1-octen-3ol, 3-octanol), some pyrazines, and pyrroles. Hence, deep
cultivation of these fungi allows obtaining natural mushroom flavorings for use in
the food industry (Krings, and Berger 1998; Longo and Sanroman 2006).
Ceratocystis, Trichoderma, Eremothecium, Pichia, and Saccharomyces genera
are of special interest for the extraction of essential oil (Christen 1995; Haeusler and
Muench 1998; Gordente et al. 2012). Quantity of aroma-forming compounds, syn-
thetized by fungi, may vary from hundreds of µg (C. populina) to hundreds of mg
(C. variospora, C. moniliformis, E. asbyi, T. viride) —per liter of cultural liquid.
Ascomycetes Ceratocystis sp. and Eremothecium sp. produce the highest amount
of aroma-forming compounds during the minimal fermentation time. For instance,
C. variospora can synthetize up to 1 g of essential oil per liter of cultural liquid on
the fifth day of cultivation. Different basidiomycetes give the following yield:
Bjerkandera adusta, 30 mg on 24th day; Lepista irina, 3–81 mg on the 28th day;
and Lentinus lepideus, 100 mg in the 15th week.
Thus, microorganisms can synthetize a wide range of chemical compounds with
a variety of scents: woody, fruity, creamy, earthy, and so on. That is why they are
important alternative sources of essential oils and individual volatile fragrant
compounds. In addition, further chemosystematic study of bioobjects, development
of biotechnology, and its introduction in industry are required.
15 Eremothecium Oil Biotechnology as a Novel Technology for the Modern… 405
Table 15.2 Yeast and yeast-like fungi which synthesize volatile fragrant compounds with
fruity-floral scent
Species Taxonomic status Synthesized substances Odor
Geotrichum candidum Fungi, Ascomycota, Ethyl isobutyrate, Fruity
(Staron) Saccharomycetes, ethyl-2-methyl butyrate,
Endomycetaceae ethyl-3-methyl butyrate
Geotrichum candidum Fungi, Ascomycota, Ethyl acetate, 3-methyl Melon
Saccharomycetes, butanol, 3-methylbutyl
Endomycetaceae acetate, β-phenylethanol,
β-phenylethyl acetate
Geotrichum penicillatum Fungi, Ascomycota, Ethyl esters, ethyl-2- Fruity
(syn. Trichosporon Saccharomycetes, methyl butyrate,
penicillatum) Endomycetaceae ethyl-3-methyl butyrate,
ethyl isobutyrate, ethyl
butyrate
Dipodascus magnusii Fungi, Ascomycota, Higher alcohols and Apple,
Saccharomycetes, esters fruity
Dipodascaceae
Hansenula anomala Fungi, Ascomycota, Ethyl acetate, isobutyl Fruity-
(syn. Pichia anomala) Saccharomycetes, acetate, 3-methylbutyl floral
Saccharomycetaceae acetate, phenylethyl
acetate, phenylethanol
Hansenula mrakii Fungi, Ascomycota, 2-Methylbutyl acetate, Fruity,
Saccharomycetes, 3-methylbutyl acetate, banana
Saccharomycetaceae isobutyl acetate
Saccharomyces Fungi, Ascomycota, Linalool, nerolidol, Floral
fermentati Saccharomycetes, trans-farnesol
Saccharomycetaceae
Saccharomyces rosei Fungi, Ascomycota, β-Myrcene, limonene, Floral,
Saccharomycetes, linalool, α-terpineol, fruity-
Saccharomycetaceae farnesol floral
Zygosaccharomyces Fungi, Ascomycota, 4-Hydroxy-2(or Intensive
rouxii Saccharomycetes, 5)-ethyl-5(or sweet
Saccharomycetaceae 2)-3(2Н)-furanone,
furaneol
Schizosaccharomyces Fungi, Ascomycota, Vanillin Vanilla
pombe Schizosaccharomycetes,
Schizosaccharomycetaceae
Sporobolomyces odorus Fungi, Basidiomycota, 4-Decanolide, Intensive
(syn. Sporidiobolus Microbotryomycetes, 5-decanolide, cis-7- peach
salmonicolor) Sporidiobolaceae decen-5-olide,
cis-6-dodecen-4-one
Sporobolomyces roseus Fungi, Basidiomycota, 4-Decalactone Peach
Microbotryomycetes,
Sporidiobolaceae
Krings and Berger (1998); Longo and Sanroman (2006); Bicas et al. (2010); Шпичка and
Семенова (2013b); Mitishev et al. (2016); Alchihab et al. (2010); Carrau et al. (2005); our data
406 E. F. Semenova et al.
Table 15.3 Mycelial fungi which synthesize volatile fragrant compounds with fruity-floral scent
Species Taxonomic status Synthesized substances Odor
Bjerkandera Fungi, 4-Methoxybenzaldehyde, Sweet,
adusta (syn. Basidiomycota, 3,4-dimethoxybenzaldehyde, odorous,
Polyporus adustus) Agaricomycetes, 3,4-dimethoxybenzyl alcohols, vanilla-like
Polyporaceae 4-decanolide
Lentinus lepideus Fungi, Methyl cinnamate, cinnamic acid, Fruity,
Basidiomycota, sesquiterpenes with cadinane structure, odorous,
Agaricomycetes, cadinol, muurolol, cubenols, farnesol, anisic,
Polyporaceae drimenol, sesquiterpene esters with cedar
murolan structure, terrestrol
Polyporus durus Fungi, 4-Butanolide, 4-pentanolide, 3-penten- Coconut,
Basidiomycota, 4-olide, 4-hexanolide, 2-hexen-4-olide, pineapple
Agaricomycetes, 5-hexen-4-olide, 5-hexanolide,
Polyporaceae 2-hepten-4-olide, 4-heptanolide,
4-octanolide, 2-nonen-4-olide,
2-decen-4-olide, 4-decanolide,
sesquiterpenes
Polyporus Fungi, Methyl benzoate, ethyl benzoate, Fruity-
tuberaster Basidiomycota, benzaldehyde floral with
Agaricomycetes, ylang-ylang
Polyporaceae notes
Pycnoporus Fungi, Vanillin, methyl anthranilate Vanilla
cinnabarinus Basidiomycota,
Agaricomycetes,
Polyporaceae
Trametes odorata Fungi, Methylanisate, anisaldehyde, Anise-like
(syn. Basidiomycota, δ-cadinene
Gloeophyllum Agaricomycetes,
odoratum; syn. Polyporaceae
Osmoporus
odoratus)
Wolfiporia cocos Fungi, Linalool Lily of the
Basidiomycota, valley
Agaricomycetes,
Polyporaceae
Gloeophyllum Fungi, Drimenol, methyl-3-hydroxy-3,7- Pleasant,
odoratum Basidiomycota, dimethyl-6-octenoate, 1-octen-3-ol fruity
Agaricomycetes,
Gloeophyllaceae
Ischnoderma Fungi, Benzaldehyde, Almond,
benzoinum Basidiomycota, 4-methoxybenzaldehyde, hawthorn
Agaricomycetes β-phenylethanol flowers
Fomitopsidaceae
Poria aurea (syn. Fungi, 2-Octen-4-olide Sweet
Auriporia aurea) Basidiomycota,
Agaricomycetes
Fomitopsidaceae
Lentinellus Fungi, Trans-nerolidol, fokienol, Anise-like
cochleatus Basidiomycota, 6-phormyl-2,2-dimethylchromene
Agaricomycetes
Auriscalpiaceae
(continued)
15 Eremothecium Oil Biotechnology as a Novel Technology for the Modern… 407
Table 15.3 (continued)
Species Taxonomic status Synthesized substances Odor
Lepista irina Fungi, (3S, 4S, Iris oil,
Basidiomycota, 10R)-3,10-epoxy-11-oxobisabola-1,8- orange
Agaricomycetes diene—lepistirone flowers
Tricholomataceae
Mycoacia uda Fungi, p-Tolualdehyde, р-methyl Fruity
Basidiomycota, acetophenone, р-methyl benzyl
Agaricomycetes alcohol, р-tolyl-1-ethanol
Meruliaceae
Phlebia radiata Fungi, 4-Decanolide Fruity with
Basidiomycota, peach note
Agaricomycetes
Meruliaceae
Oospora Fungi, Amino acid esters Fruity
suaveolens Basidiomycota,
Agaricomycetes
Botryobasidiaceae
Pleurotus euosmus Fungi, Linalool, coumarin, cis- and trans- Sweet,
Basidiomycota, linalool oxides floral
Agaricomycetes
Pleurotaceae
Cystostereum Fungi, 1-Octen-3-one, benzofuran terpenoids, Vanilla,
muraii Basidiomycota, bisabolan coconut
Basidiomycetes, flakes
Cystostereaceae
Tyromyces Fungi, 4-Decalactone (4-decanolide), other Peach,
sambuceus Basidiomycota, lactones passion
Basidiomycetes, fruit,
Polyporaceae coconut
Aspergillus oryzae Fungi, Ascomycota, 1-Octen-1-ol Pineapple
Eurotiomycetes,
Trichocomaceae
Aspergillus terreus Fungi, Ascomycota, Ethyl acetate Fruity
Eurotiomycetes,
Trichocomaceae
Trichothecium Fungi, Ascomycota, Nerol, linalool, citronellol, terpineol, Floral,
roseum Ascomycetes, nerolidol, linalyl acetate, citronellyl mushroom
Incertae sedis acetate, geranyl acetate, 1-octen-3-ol,
3-octanol, 1,5-octadien-3-ol, octan-
1-ol, 2-octen-1-ol
Ceratocystis Fungi, Ascomycota, 6-Methyl-5-hepten-2-on, 6-methyl-5- Fruity
coerulescens Sordariomycetes, hepten-2-ol, nerolidol, citronellol,
Ophiostomataceae citronellyl acetate, 2,3-dihydrofarnesol,
trans-farnesol, geraniol, geranyl
acetate, nerol, linalool, α-terpineol,
neryl acetate
Ceratocystis Fungi, Ascomycota, Linalool, citronellol, geraniol, Sweet,
fimbriata Sordariomycetes, α-terpineol fruity
Ophiostomataceae
(continued)
408 E. F. Semenova et al.
Table 15.3 (continued)
Species Taxonomic status Synthesized substances Odor
Ceratocystis Fungi, Ascomycota, Bicyclic sesquiterpenes with Pleasant,
populina Sordariomycetes, 1,7-dimethyl-4-isopropyldecaline fruity
Ophiostomataceae skeleton, δ-cadinol, δ-cadinene
Ceratocystis Fungi, Ascomycota, Vitronellol, citronellyl acetate, Odorous,
variospora Sordariomycetes, geranial, neral, geraniol, linalool, geranium-
Ophiostomataceae geranyl acetate, nerol, α-terpineol like
Leptographium Fungi, Ascomycota, Sesquiterpene alcohols with africanane Sweet,
lundbergii Sordariomycetes, skeleton (africanols): leptografiol, fruity
Ophiostomataceae isoleptografiol, isoafricanol
Fusarium pore Fungi, Ascomycota, τ-Lactones, τ-decalactone, Fruity,
Sordariomycetes, (Z)-6-τ-dodecenolactone peach
Nectriaceae
Hypomyces Fungi, Ascomycota, Sesquiterpene esters and alcohols, Camphor-
odoratus Sordariomycetes, 1-octen-3-ol like
Hypocreaceae
Trichoderma Fungi, Ascomycota, 6-Pentyl-α-pyrone Coconut
koningii Sordariomycetes,
Hypocreaceae
Trichoderma Fungi, Ascomycota, 6-Pentyl-2-pyrone Coconut
reesei Sordariomycetes,
Hypocreaceae
Trichoderma Fungi, Ascomycota, 6-Pentyl-2-pyrone, Coconut
viride Sordariomycetes, 6-(pent-1-enyl)-2-pyrone
Hypocreaceae
Cladosporium Fungi, Ascomycota, Isobutyl alcohol, isobutyl acetate, Fruity
cladosporioides Dothideomycetes, 3-methyl-butanol, 3-methylbutyl
Davidiellaceae acetate, β-phenylethanol, β-phenyl
acetate
Cladosporium Fungi, Ascomycota, γ-Decalactone, δ-dodecalactone Coconut
suaveolens Dothideomycetes,
Davidiellaceae
Monilia fruticola Fungi, Ascomycota, 4-Octalactone, 4-decalactone Peach
Leotiomycetes,
Sclerotiniaceae
Etschmann et al. (2002); Medeiros et al. (2006); Шпичка and Семенова (2013b); our data
15.1.2 S
creening of Bioobjects with Different Taxonomic
Statuses Which Produce Rose-Scented Essential Oil
It was revealed that oil content in rose cell culture is significantly less than in intact
petals. Furthermore, composition of extractable oil differs from phytogenous rose
oil (Mulder-Krieger et al. 1988; Егорова and Ставцева 2006). During the period
1980–1990, microorganisms were used to extract natural fragrant substances
for the first time. Among all the studied organisms, a group which produces
rose-scented alcohols and esters was singled out Шпичка and Семенова 2015a
(Tables 15.4 and 15.5).
Research conducted on objects promising for aroma product biotechnology
revealed the differences in the biosynthetic activity and essential oil composition
between species and strains (Шпичка and Семенова 2013a). To extract rose-scented
essential oil, researchers were interested in the genera Ceratocystis, Eremothecium,
Pichia, and Saccharomyces. Considerable part of volatile fragrant compounds, syn-
thetized by them, are floral-scented, generally with rose scent. In most of the micro-
organisms, this odor is caused by β-phenylethanol. The latter is synthetized in
enzymatic reactions of deamination, decarboxylation, and oxidation of
L-phenylalanine (Бугорский et al. 1986). However, Ceratocystis sp., Eremothecium
Table 15.4 Mycelial fungi which synthesize volatile fragrant compounds with roselike scent
Species Taxonomic status Synthesized substances Odor
Inоcybe Fungi, Basidiomycota, Methyl cinnamate Fruity, roselike
сoridalina, I. Agaricomycetes,
pyrlodora, I. Cortinariaceae
odorata
Mycena pura Fungi, Basidiomycota, Citronellol Rose
Agaricomycetes,
Tricholomataceae
Aspergillus Fungi, Ascomycota, Methyl ketones, Unpleasant,
niger Eurotiomycetes, β-phenylethanol roselike
Trichocomaceae
Penicillium Fungi, Ascomycota, Thujopsene, 3-octanone, Pine-like,
decumbens Eurotiomycetes, nerolidol, 1-octen-3-ol, roselike,
Trichocomaceae β-phenylethanol apple-like,
mushroomlike
Ceratocystis Fungi, Ascomycota, 3-Methylbutyl acetate, geraniol, Banana, pear,
moniliformis Sordariomycetes citronellol, nerol, linalool, rose, peach
Ophiostomataceae α-terpineol, geranial, neral,
citronellyl acetate, geranyl
acetate
Ceratocystis Fungi, Ascomycota, Citronellol, geraniol, linalool, Fruity, roselike
virescens Sordariomycetes geranyl acetate, nerol,
Ophiostomataceae α-terpineol, geranial, neral,
citronellyl acetate, neryl acetate
Ascoidea Fungi, Ascomycota, β-Phenylethanol, furan-2- Fruity-floral,
hylecoeti Saccharomecetes, carboxylic acid, citronellol, roselike
Ascoidaceae nerol, linalool, α-terpineol,
citronellal, limonene, myrcene,
citronellyl acetate
Janssens et al. (1992); Krings and Berger (1998); Шпичка and Семенова (2013b); our data
410 E. F. Semenova et al.
Table 15.5 Yeast and yeast-like fungi which synthesize volatile fragrant compounds with roselike
scent
Species Taxonomic status Synthesized substances Odor
Ambrosiozyma Fungi, Ascomycota, Geraniol, citronellol, nerol, Roselike
cicatricosa, А. Saccharomycetes, β-phenylethanol, α-terpineol,
monospora Saccharomycopsidaceae citral, linalool
Eremothecium Fungi, Ascomycota, Geraniol, citronellol, nerol, Roselike
ashbyi, Е. gossypii Saccharomycetes, β-phenylethanol, linalool,
Eremotheciaceae citral, farnesol
Hansenula Fungi, Ascomycota, Ethyl acetate, 3-methylbutanol, Roselike
saturnus Saccharomycetes, 3-methylbutyl acetate,
Saccharomycetaceae β-phenylethanol, 2-phenylethyl
acetate
Kluyveromyces Fungi, Ascomycota, Citronellol, geraniol, linalool, Roselike,
lactis Saccharomycetes, β-phenylethanol, esters, fruity, floral
Saccharomycetaceae isoamyl alcohol, acetoin,
2-phenyl acetate, isobutanol,
isovaleric acid
Kluyveromyces Fungi, Ascomycota, β-Phenylethanol Roselike
marxianus Saccharomycetes,
Saccharomycetaceae
Pichia farinosa Fungi, Ascomycota, Ethyl acetate, 3-methylbutanol, Roselike
Saccharomycetes, 3-methylbutyl acetate,
Saccharomycetaceae β-phenylethanol, 2-phenylethyl
acetate
Pichia fermentans Fungi, Ascomycota, β-Phenylethanol Roselike
Saccharomycetes,
Saccharomycetaceae
Saccharomyces Fungi, Ascomycota, β-Phenylethanol, 4-decanolide; Roselike,
cerevisiae Saccharomycetes, linalool, geraniol, citronellol, fruity-floral,
Saccharomycetaceae α-terpineol; vanillin vanilla
Saccharomyces Fungi, Ascomycota, β-Phenylethanol Roselike
vini Saccharomycetes,
Saccharomycetaceae
Torulopsis utilis Fungi, Ascomycota, β-Phenylethanol, ethyl acetate Roselike
(syn. Candida Saccharomycetes,
utilis) Saccharomycetaceae
Etschmann et al. (2002), Longo and Sanroman (2006), Mitrović et al. (2011), Шпичка and
Семенова (2013b); our data
sp., and Kluyveromyces sp. can also synthesize terpene alcohols (geraniol, citronel-
lol, nerol, linalool, farnesol), which are the main components of rose essential oil
(Родов et al., 1987).
Monoterpene alcohols can be extracted during cultivation of these producers, so
there are some specified pathways of its biosynthesis. These pathways are associated
with the formation of isopentenyl diphosphate—precursor of terpenes and terpenoids
(Klein-Marcuschamer et al. 2007; Schwab et al. 2008; Baydar and Baydar 2013;
Langenheim 1994). Understanding the mechanisms underlying the isopentenyl
diphosphate synthesis pathways and isomerization conditions of unstable
15 Eremothecium Oil Biotechnology as a Novel Technology for the Modern… 411
Table 15.6 Composition of Eremothecium and rose essential oil, average value
Mass concentration, %
Organism Aroma product MTAc Geraniol Citronellol Nerol β-Phenylethanol
E. ashbyi 1a 61.9–77.6 65.5–80.9 6.0–11.4 1.8–3.4 21.7–37.5
2b 78.0–84.9 43.3–64.2 2.6–5.1 0.5–3.5 9.8–12.7
E. gossypii 1 56.7–66.4 31.5–69.7 0.3–4.6 0.1–6.8 33.1–43.2
2 52.8–61.9 35.0–52.4 1.2–2.8 0.2–2.7 37.3–46.3
Oil- 1 (OST 10–60–87) ≥8.0 – – – 75.0–88.0
bearing 2 (GOST ≥8.0 – – – 75.0–88.0
rose 31,791–2012)
Note: a1 hydrodistillated essential oil, b2 extracted essential oil, cMTA monoterpene alcohols
Шпичка and Семенова (2016a)
412 E. F. Semenova et al.
15.1.3 S
tructural and Functional Aspects of Eremothecium
ashbyi and Eremothecium gossypii Ontogenesis
Fig. 15.1 Life, sexual reproduction; internal circle, asexual reproduction (conidium formation);
left circle, asexual reproduction (budding) (Семенова et al. 2013)
15 Eremothecium Oil Biotechnology as a Novel Technology for the Modern… 413
Fig. 15.2 Mycelium structure of Eremothecium sp.: dichotomous branching of hypha ((a), E. ash-
byi; (c), E. gossypii), septation (arrow points at intercellular septum, (b), E. ashbyi; (d), E. gossy-
pii) (Семенова et al. 2013)
capable of this process. However, this opinion was not approved or disproved in
other scientific literature. Asexual reproduction of studied microorganisms can
also occur via budding cells (Fig. 15.3) that can grow and form a separate myce-
lium. These cells can generate yeast phase of micromycete development in case we
change the cultivation method. For example, it is possible if we reduce the supply
of oxygen to the liquid medium and do not allow it to spread evenly. These condi-
tions can be created if there is no shaking during cultivation or the shaking rates
are significantly reduced. Thus, studied species tend to dimorph. This was not
noted in previous cytomorphologic investigations. It also disproves the statement
made by M. Nadal et al. (2008). They supposed these microorganisms to have only
mycelial growth.
During sexual reproduction, special unicellular structures (meiosporangia) are
formed. Sporangia of E. ashbyi are oblong (65.2–90.8 × 14.7–20.2 mkm), E. gos-
sypii—clavate, cylindrical, or sigmoid (86.8–157.2 × 12.4–19.6 mkm) (Fig. 15.4).
It was noted, that asci can be located singly or in groups and chains. A specified
number of separate ascospores (Бугорский et al., 1986; Быков et al., 2003;
Величко et al., 2015; Войткевич, 1999; Гуринович & Пучкова, 2005; Егорова &
Ставцева, 2006; Жученко et al., 2015; Маркелова et al., 2014; Митишев et al.,
2014; Митишев et al., 2017; Погорельская et al., 1999; Родов et al., 1987;
Семенова, 2007) develop endogenously in sporangium. Spores grow after being
414 E. F. Semenova et al.
Fig. 15.3 Structures for asexual reproduction: spindle-shaped conidia, which are located laterally
on hypha ((a), E. ashbyi; (b), E. gossypii); budding yeast-like cells (c, d) (Семенова et al. 2013)
Fig. 15.4 Structures for sexual reproduction: (a) nuclei fusion in hypha of mycelium (pointed by
arrows; single nucleus is pointed by dashed arrow); (b) chain formed by 3 asci and single ascus;
(c) rupture of ascus and releasing of ascospores; (d and e) polysporous single sporangia and asco-
spores of E. ashbyi; (f and g) polysporous single sporangia and ascospores of E. gossypii
(Семенова et al. 2013)
15 Eremothecium Oil Biotechnology as a Novel Technology for the Modern… 415
released via rupture of seed coat. They are species-specific: E. ashbyi has simple
spores that are clavate and needle-shaped, straight, or bent with a narrow tip and
granule-free (20.1–27.2 × 2.4–2.9 mkm). E. gossypii have spindle-shaped or nee-
dle-shaped ascospores, often with thin septum in the middle (17.5–45.8 × 1.3–
4.6 mkm) (Fig. 15.4).
It should be noted that reproduction process is different for studied species of
microorganisms (Figs. 15.5 and 15.6). In the life cycle of E. ashbyi, there is no spore
Fig. 15.5 Diagram of sexual and asexual reproduction of E. ashbyi (Семенова et al. 2013)
Fig. 15.6 Diagram of sexual and asexual reproduction of E. gossypii (Семенова et al. 2013)
416 E. F. Semenova et al.
Fig. 15.7 Dynamics of essential oil and riboflavin accumulation during E. ashbyi ontogenesis: culti-
vation stages, starting from spore germination (ЭМ, essential oil; В2, riboflavin) (Семенова et al. 2013)
and systematizes knowledge of E. ashbyi and E. gossypii growth and life cycles and
structural and functional alterations connected with it. This analysis can be used in
mycological and phytopathological studies of mentioned objects.
15.1.4 A
romatic and Monoterpene Alcohol Accumulation
by Eremothecium ashbyi Strains Differing
in Riboflavinogenesis
In our study, we compared different E. ashbyi strains: industrial VKPM F-340, yel-
low VKM F-3009, and white VKM F-124 (a mutant obtained by E. F. Semenova
(Cеменова et al. 2013). The color intensity of strain colonies differed depending on
the riboflavinogenesis level (Revuelta et al. 2017).
We examined the dynamics of biomass accumulation during strain submerged
cultivation and revealed no significant differences from the known regularities.
The log phase of growth was observed up to 36 h and followed by a slowdown in
growth rate (transition to stationary phase). Cell autolysis occurred at the end of
fermentation (Fig. 15.9).
Overall, 60-h cultivation of strains VKPM F-340 and VKM F-3009 and a white
mutant of strain VKM F-124 resulted in achieving minimum (1.06 g × L–1), medium
418 E. F. Semenova et al.
Fig. 15.8 Morphological alterations connected with biosynthetical activity: thickening and vacu-
olization of hypha (1, E. ashbyi; 2, E. gossypii); lipidic droplets within the mycelium (pointed with
arrow, 3, E. ashbyi; 4, E. gossypii); crystalline inclusions of riboflavin within hypha vacuoles
(pointed with arrow, 5, E. ashbyi; 6, E. gossypii) (Семенова et al. 2013)
Fig. 15.9 (a) Dry biomass accumulation and (b) pH changes of CB during submerged cultivation
of (1) VKM F-124, (2) VKM F-3009, and (3) VKPM F-340 strains of E. ashbyi
Fig. 15.10 Accumulation
(mg × g–1) of vitamin B2
during cultivation of (1)
VKPM F-340, (2) VKM
F-3009, and (3) VKM
F-124 strains of E. ashbyi
by the changes in acid-base balance of the accumulated biomass and the intensity of
alkaline metabolite excretion.
The riboflavin synthesis and accumulation in culture medium began in the log
phase and increased during stationary and decline phases (Fig. 15.10). The riboflavin
synthesis by strain VKPM F-340 was the most intense. Strain VKM F-3009 and the
white mutant of strain VKM F-124 had less intense riboflavin synthesis (three and
ten times, respectively) than strain VKM F-340.
The intensity of accumulation of the main aroma-forming compounds (geraniol,
citronellol, nerol, and 2-phenylethanol) was different among these strains Шпичка
and Семенова 2014. The most intense accumulation of these compounds was
observed in the stationary phase (Figs. 15.11 and 15.12).
Significant differences in geraniol synthesis were noticed at certain stages of
culture development. The highest increase in geraniol accumulation by strain VKM
420 E. F. Semenova et al.
Fig. 15.11 Accumulation
(mg × g–1) of (1) geraniol
and (2) β-phenylethanol
during submerged
cultivation of (a) VKM
F-3009, (b) VKPM F-340,
and (c) VKM F-124 strains
of E. ashbyi
15 Eremothecium Oil Biotechnology as a Novel Technology for the Modern… 421
Fig. 15.12 Accumulation
(mg × g–1) of (a) citronellol
and (b) nerol during
submerged cultivation of
(1) VKPM F-340, (2)
VKM F-3009, and (3)
VKM F-124 strains of
E. ashbyi
F- 3009 was observed between 48 and 60 h, while for strain VKPM F-340 and VKM
F-124 it was between 48 and 60 h. Nevertheless, the latter had the insignificant
geraniol increase.
It was noted above that in 24 h, essential oil and riboflavin began to accumulate
within mycelia and in medium, and lipid bodies formed in micromycete vegetative
hyphae (Fig. 15.13). Among all strains, the increase in the essential oil synthesis
efficiency (Figs. 15.11 and 15.12) was accompanied with the growth in spherosome
quantities and sizes.
The marked vacuolization (after 36–48 h) and the beginning of sporogenesis
(after 48–60 h) were observed during the period of highest accumulation of aroma-
forming compounds and vitamin B2 (Fig. 15.14).
Strain VKPM F-340, which possessed the highest intensity of essential oil syn-
thesis, had more intense vacuolization (numerous small vacuoles) than other strains.
The vacuolization of this strain began at earlier stages and finished at the later stages
of growth than that of other strains. Strain VKPM F-340 possessed more intense
protoplasm lipophilicity (osmophilicity in electron microscopy) than other strains
(Fig. 15.15).
422 E. F. Semenova et al.
Fig. 15.14 Mycelium of E. ashbyi during (a) log phase, (b) a slowdown in the growth, (c) station-
ary phase, and (d) at the beginning of decline phase
Fig. 15.15 Cytomorphological features of Eremothecium mycelium and alteration of the main
biologically active substance accumulation: I, riboflavin; II, essential oil; III, geraniol; a1, a2 36 h;
b1, b2 48 h of cultivation in liquid medium (magnification: a1, b1,– ×100; a2, ×18500; b2, ×20000)
(Семенова et al. 2015)
the riboflavin and essential oil biogenesis in E. ashbyi and includes basic biochemi-
cal reactions for correlation determination among all reactions (Fig. 15.16).
According to this model, fatty acids, glucose, aspartate, asparagine, glycine, ser-
ine, and threonine are the main substrates for the riboflavin synthesis, whereas fatty
acids, glucose, and leucine are substrates for the monoterpene alcohol synthesis (gera-
niol, nerol, and citronellol); phenylalanine participates in the aromatic alcohol synthe-
sis (β-phenylethanol). Acetyl-CoA, which is formed during glucose and fatty acid
catabolism, is a key compound in vitamin and monoterpene synthesis. This metabolic
model enables us to understand the relationships among the processes of the synthesis
of flavins and terpene and aromatic alcohols, their direction, cellular localization, and
the regulation mechanisms of E. ashbyi productivity Бугорский et al. 1990.
Figures 15.10, 15.11, and 15.12 show that strain VKPM F-340 had the highest
vitamin and aroma-forming compound productivity. However, our study of the
dynamics of formation of these compounds revealed that in 60 h, the accumulation
of monoterpenes had no changes and remained approximately the same, while that
of riboflavin continued to increase. This might have been caused by the decreased
424 E. F. Semenova et al.
Fig. 15.16 Hypothetical metabolic model of riboflavin, phenylethanol, and terpene alcohol bio-
synthesis by E. ashbyi cells
Table 15.7 Spearman’s correlation coefficients between riboflavin and monoterpene alcohol
syntheses by E. ashbyi strains
Correlation coefficients
Producer
VKM VKM VKPM Significance level
Compound F-124 F-3009 F-340 range
Geraniol 0.81 0.94 0.94 0.005–0.052
Citronellol 0.97 1.00 0.94 0.001–0.050
Nerol – 1.00 0.94 0.005–0.050
Geraniol + citronellol 0.90 0.94 0.94 0.005–0.015
Geraniol + nerol 0.81 1.00 0.94 0.005–0.052
Citronellol + nerol 0.97 1.00 0.94 0.005–0.050
MTAa 0.90 1.00 0.94 0.005–0.050
Phenylethanol/ −0.90 −0.94 −0.94 0.000–0.005
(citronellol + nerol)
Note. aMTA monoterpene alcohols, “–” means that the compound was not detected
Table 15.9 Comparative analysis of aroma products synthetized by Eremothecium strains and
Rosa species
Ratio Efficiency of oil
β-Phenyl- Geraniol/ production process, mg
Producer ethanol/ MTAa citronellol Geraniol/ erol per g of biomass/h
E. ashbyi
ВКМ F-3009 0.02–0.12 9.12–15.30 13,6–24.65 0.813–1.298
ВКМ F-4565 0.01–0.37 4.66–12.21 15.64–48.86 1.032–1.682
ВКМ F-4566 0.08–0.31 0.98–6.90 2.22–10.17 0.825–1.237
ВКПМ F-36 0.22–0.39 2.51–7.04 3.65–68.20 0.930–1.358
ВКПМ F-340 0.11–0.24 37.62–51.94 – 0.976–1.240
ВКПМ F-1320 0.79–1.41 20.50–58.89 0.00–29.45 1.347–2.334
E. gossypii
ВКМ F-2627 1.12–1.27 13.92–77.33 6.96–34.72 1.514–1.915
ВКМ F-3276 0.79–1.29 7.76–13.21 4.79–26.42 0.627–2.198
ВКМ F-3296 0.01–16.82 1.53–8.31 0.00–39.73 0.326–0.759
ВКПМ F-1321 0.85–1.53 9.31–31.20 6.72–46.8 0.873–2.066
Essential oil-bearing Rosa species
R. alba 0.05–0.13 0.26–1.20 1.43–3.20 0.002–0.003
R. gallica 2.33–3.00 2.00–2.40 1.00–4.20 0.004–0.006
R. damascenа 0.04–0.08 0.13–1.12 0.55–7.67 0.001–0.005
Note. aMTA monoterpene alcohols, “–” means that the compound was not detected
Table 15.10 Biological activity of Eremothecium oil against L. lactis spp. lactis and L. аcidophilus
Indicators of delay or absence of culture growth
Dilution of L. lactis spp.lactis L. acidophilus
Eremothecium oil Lim, mm CV, % x̅ + Sх, mm Lim, mm CV, % x̅ + Sх, mm
I 10…20 26.9 16,0 ± 4.3 12...15 9.5 13.7 ± 1.3
II 5…9 45.5 7.5 ± 2.5 8...10 11.1 9,0 ± 1.0
III 5…7 16.7 6,0 ± 1,0 6…11 29.4 8.5 ± 2.5
linalool (R = −0.74; strong negative association), and total monoterpene alcohol
(R = 0.5; moderate positive association) content (Table 15.13 and Fig. 15.18). We
revealed the weak negative correlation between sample activity on P. caudatum and
citronellol (R = −0.19) content.
The content of the aroma-forming compounds and their combination, which pos-
sessed additional inhibitory activity, causes the antimicrobial and toxic action of
Eremothecium oil Markelova and Semenova 2017. These data correspond with
those for essential oil from rose petals (Семенова 2007; Маркелова et al. 2014;
Величко et al. 2015).
428 E. F. Semenova et al.
Fig. 15.17 Growth curves of test bacteria (1, in a medium containing Eremothecium oil in bacte-
riostatic concentration 2107, 81 mkg ml−1; 2, in a medium without essential oil); (a) S. aureus; (b)
E. coli; x-axis, measurement time (hours); y-axis, optical density increment (Янина et al. 2017)
15 Eremothecium Oil Biotechnology as a Novel Technology for the Modern… 429
15.3 Conclusion
Essential oils are widely spread in the vegetable world, from fungi and algae to
flowering plants. Earth shelters around 3000 plants that can be used a source of
essential oil. But nowadays, a limited number of higher plants are used in industries.
The quality of essential oil depends considerably on ecological factors such as
430 E. F. Semenova et al.
Fig. 15.18 P. caudatum viability dynamics in media containing Eremothecium oil of different
origins with concentration 210 mkg ml−1 (samples (а) 1; (b) 2; (c) 3; (d) 4)
References
Гуринович ЛК, Пучкова ТВ (2005) Эфирные масла: химия, технология, анализ и применение.
Школа Косметических Химиков, Москва
Егорова НА, Ставцева ИВ (2006) Некоторые итоги и перспективы биотехнологических
исследований эфиромасличных растений. Научные труды Института эфиромасличных
и лекарственных растений 26:19–26
Жученко ЕВ, Семенова ЕФ, Маркелова НА, Шпичка АИ (2015) Влияние эфирных
масел на микроорганизмы различной таксономической принадлежности в сравнении
с современными антибиотиками. Сообщение 3. Действие масел лаванды, розового
дерева, эвкалипта, пихты на некоторые грамотрицательные бактерии. Известия высших
учебных заведений. Поволжский регион. Серия «Естественные науки» 1(9):30–41
Маркелова НА, Семенова ЕФ, Шпичка АИ, Жученко ЕВ (2014) Влияние эфирных масел
на микроорганизмы различной таксономической принадлежности в сравнении с
современными антибиотиками. Сообщение 1. Действие розового эфирного масла и
антибиотических субстанций на некоторые грамотрицательные бактерии. Известия
высших учебных заведений. Поволжский регион. Серия «Естественные науки»
3(7):39–48
Митишев АВ, Преснякова ЕВ, Семенова ЕФ, Гурина МА (2014) Сравнительный
анализ штаммов продуцента и инновационного продукта как основных элементов
биотехнологии резиноида хлореллы. Известия высших учебных заведений. Поволжский
регион. Серия «Естественные науки» 4(8):19–29
Митишев АВ, Семенова ЕФ, Преснякова ЕВ (2017) К вопросу разработки технологии
культивирования и переработки Chlоrellа vulgаris для получения резиноида. Вестник
Воронежского университета. Серия «Химия. Биология. Фармация» 4:62–65
Погорельская АН, Кочетков ЕС, Бугорский ПС, Семенова ЕФ (1999) Биотехнологические
методы в помощь создания рентабельного эфиромасличного производства с наиболее
полной утилизацией отходов. Труды Института эфиромасличных и лекарственных
растений 25:170–180
Родов ВС, Бугорский ПС, Семенова ЕФ (1987) Получение эфирного масла розового
направления на основе биотехнологии. Труды ВНИИ эфиромасличных культур 18:13–15
Семенова ЕФ (2007) Биосинтетическая активность и антимикробные свойства Eremothecium
ashbyi Guill. Известия вузов. Поволжский регион. Серия «Медицинские науки» 4:44–50
Семенова ЕФ, Богданов НИ (2000) Некоторые результаты биотехнологии ароматических
продуктов. Инновационные технологии и продукты 4:9–13
Семенова ЕФ, Бугорский ПС (1989) Некоторые итоги поиска биотехнологически
перспективных ароматообразующих культур. Труды ВНИИ эфиромасличных культур
20:14–16
Семенова ЕФ, Бугорский ПС (1990) К вопросу утилизации отходов эфиромасличного
производства. Труды/ ВНИИ эфиромасличных культур 21:179–183
Семенова ЕФ, Преснякова ЕВ, Жужжалова ТП (2014) Репродуктивная биология видов и
форм Rosa L. Изд-во ЦНТИ, Воронеж
Семенова ЕФ, Шпичка АИ, Моисеева ИЯ (2011) Культурально-морфологические и
физиолого-биохимические свойства видов рода Eremothecium. Фундаментальные науки
6:210–214
Семенова ЕФ, Шпичка АИ, Преснякова ЕВ, Меженная НА (2015) Структурные основы
маслонакопления в лепестках некоторых видов рода Rosa (Rosaceae) и мицелии
аскомицетов Eremothecium (Eremotheciaceae). Растительные ресурсы 51(4):500–509
Семенова ЕФ, Шпичка АИ, Преснякова ЕВ (2013) Фармацевтическая микология видов рода
Eremothecium (Биология, технология, практическое использование). Изд-во ПГУ, Пенза
Семенова ЕФ, Шпичка АИ (2014) О методических аспектах селекции продуцентов
эфирного масла розового направления запаха. Вестник Воронежского госуниверситета.
Серия «Химия. Биология. Фармация» 2:91–95
Семенова ЕФ, Шпичка АИ (2012) Фармбиотехнологическая характеристика Eremothecium –
продуцента рибофлавина и эфирного масла. Разработка, исследование и маркетинг
новой фармацевтической продукции 67:368–372
15 Eremothecium Oil Biotechnology as a Novel Technology for the Modern… 433
Gordente AG, Curtin CD, Varela C, Pretorius IS (2012) Flavor-active wine yeast. Appl Microbiol
Biotechnol 66:1–18
Haeusler A, Muench T (1998) Microbial production of natural flavors. ASM News 63(10):551–559
Hansen EH, Lindberg Moller B, Kock GR, Buenner CM, Kristensen C, Jensen OR, Okkels FT,
Olsen CE, Motawia MS, Hansen J (2009) De novo biosynthesis of vanillin in fission yeast and
baker’s yeast. Appl Environ Microbiol 75(9):2765–2774
Janssens L, De Pooter HL, Schamp NM, Vandamme EJ (1992) Production of flavours by microor-
ganisms. Process Biochem 27:195–215
Jianping J (1993) Identification of volatile compounds produced by Kluyveromyces lactis.
Biotechnol Tech 7(12):863–866
Kambourova R, Bankova V, Petkov G (2003) Volatile substances of the green alga Scenedesmus
incrassatulus. Z Naturforsch 53:187–190
Klein-Marcuschamer D, Ajikamar PK, Stephanopoulos G (2007) Engineering microbial cell
factories for biosynthesis of isoprenoids. Trends Biotechnol 25(9):417–424
Knyazkova АА, Semenova EF, Shpichka AI, Velichko VP, Bezrukova EI (2016) About the main-
tenance in the active state of high-productive variant of Eremothecium ashbyi. Top Issues New
Drugs Dev 1:356–357
Krings U, Berger RG (1998) Biotechnological production of flavours and fragrances. App
Microbiol Biotechnol 49:1–8
Kurtzman CP (1995) Relationships among the genera Ashbya, Eremothecium, Holleya and
Nematospora determined from rDNA sequence divergence. J Ind Microbiol 14:523–530
Langenheim JH (1994) Higher plant terpenoids: a phytocentric overview of their ecological role.
J Chem Ecol 20(6):1223–1280
Ledesma-Amaro R, Kerkhoven EJ, Revuelta JL, Nieland J (2013) Genome scale metabolic model-
ing of the riboflavin overproducer Ashbya gossypii. Biotechnol Bioeng 9999:1–10
Ledesma-Amaro R, Santos M, Jimenez A (2014) Strain design of Ashbya gossypii for single-cell
oil production. Appl Environ Microbiol 80(4):1237–1244
Longo MA, Sanroman MA (2006) Production of food aroma compounds: microbial and enzy-
matic methodologies. Food Technol Biotechnol 44(3):335–353
Markelova NN, Semenova EF (2017) Sensitivity of nonfermentative gram-negative bacteria to
essential oils of different origin. Microbiologie 86(5):610–617
Marx H, Mattanovich D, Sauer M (2008) Overexpression of the riboflavin biosynthetic pathway in
Pichia pastoris. Microb Cell Factories 7:23
Medeiros ABP, Pandey A, Vandenberghe LPS, Pastore GM, Soccol CR (2006) Production and
recovery of aroma compounds produced by solid-state fermentation using different absorbents.
Food Technol Biotechnol 44(1):47–51
Misawa N (2011) Pathway engineering for functional isoprenoids. Curr Opin Biotechnol 22:1–7
Mitishev AV, Semenova EF, Velichko VP, Shpichka AI, Moiseeva IY (2016) Determination of the
biological activity of chlorella resinoids in regard to Paramecium сaudatum. Int Res J 4(46),
5:23–27
Mitrović T, Stamenković S, Cvetković V, Nikolić M, Tošić S, Stojičić D (2011) Lichens as a source
of versatile bioactive compounds. Biologica Nyssana 2(1):1–6
Mulder-Krieger T, Verpoorte R, Baerheim A, Svendsen J, Scheffer JC (1988) Production of
essential oils and flavours in plant cell and tissue cultures. A review. Plant Cell Tiss Org Cult
13:85–154
Nadal M, García-Pedrajas MD, Gold SE (2008) Dimorphism in fungal plant pathogens. FEMS
Microbiol Lett 284:127–134
Nieland S, Stahmann K-P (2013) A developmental stage of hyphal cells shows riboflavin instead
of sporulation in Ashbya gossypii. Appl Microbiol Biotechnol 97:10143–10153
Pandal N (2006) The global market for flavors and fragrances. BBC Research, London
Ravasio D, Wendland J, Walther A (2014) Major contribution of the Ehrlich pathway for the
2-phenylethanol/rose flavor production in Ashbya gossypii. FEMS Yeast Res 1:1–12
Revuelta JL, Ledesma-Amaro R, Lozano-Martinez P, Díaz-Fernández D, Buey RM, Jiménez
A (2017) Bioproduction of riboflavin: a bright yellow history. J Ind Microbiol Biotechnol
44:659–665
15 Eremothecium Oil Biotechnology as a Novel Technology for the Modern… 435
Schindler J, Schmid RD (1982) Fragrance or aroma chemicals. Microbial synthesis and enzymatic
transformation – a review. Process Biochem 17:2–8
Schiestl FP (2010) The evolution of floral scent and insect chemical communication. Ecol Lett
13(5):643–656
Schwab W, Davidovich-Rikanati R, Lewinsohn E (2008) Biosynthesis of plant-derived flavor com-
pounds. Plant J 54:712–732
Semenova Е, Presnyakova V, Goncharov D, Goncharov M, Presnyakova E, Presnyakov S,
Moiseeva I, Kolesnikova S, Moiseev J (2017a) Spectrophotometric method for quantitative
measuring essential oil in aromatic water and distillate with rose smell. J Phys: Conference
Series 784 1:012053. https://doi.org/10.1088/1742-6596/784/1/012053. http://iopscience.iop.
org/article/10.1088/1742-6596/784/1/012053/meta
Semenova EF, Rodov VS, Shpichka AI (2011) Influence of conditions of inoculating material
preparation on accumulation of aroma building substances in culture of Eremothecium ashbyi
Guill. Int J Appl Fundam Res 6:87
Semenova EF, Shpichka AI (2012) Some pharmbiotechnological characteristics of Еremothecium,
producer of riboflavin and essential oil. Int J Appl Fundam Res 1:170–172
Semenova EF, Shpichka AI, Moiseeva IY (2012a) About explanation of elaboration of essential
Eremothecium oil biotechnology. Int J Exp Educ 3:35–36
Semenova EF, Shpichka AI, Moiseeva IY (2012b) About essential oils biotechnology on the base
of microbial synthesis. Eur J Nat Hist 4:29–31
Semenova EF, Shpichka AI, Presnyakova EV, Mezhennaya NA (2016) Processes of essential oil
accumulation in petals of Rosa (Rosaceae) and mycelium of Eremothecium. Bull State Nikit
Botan Gard 118:25–33
Semenova EF, Shpichka AI, Presnyakova ЕV (2017b) Aromatic and monoterpene alcohol accu-
mulation by Eremothecium ashbyi strains differing in riboflavinogenesis. Appl Biochem
Microbiol 53(3):374–380
Semenova EF, Shpichka AI, Presniakova EV, Presniakova VS, Goncharov MA, Goncharov DA
(2017c) Development of a novel biotechnological fragrant product, Eremothecium oil. Indian
J Pharm Educ Res 51(3):136–138
Soetaert W, Vandamme EJ (2010) Industrial biotechnology: sustainable growth and economic suc-
cess. Willey, Washington
Takahashi S, Yeo J, Greenhagen BT, McMullin T, Song L, Maurina-Brunker J, Rosson R, Noel
JP (2007) Chappell Metabolic engineering of sesquiterpene metabolism in yeast. Biotechnol
Bioeng 97(1):170–181
Vandamme EJ (2003) Bioflavours and fragrances via fungi and their enzymes. Fungal Divers
13:153–166
Walther A, Wendland J (2012) Yap1-dependent oxidative stress response provides a link to riboflavin
production in Ashbya gossypii. Fungal Genet Biol 49(9):697–707
Wasserstorm L, Lengeler KB, Walther A, Wendland J (2013) Molecular determinants of sporulation
in Ashbya gossypii. Genet 195:87–99
Index
A extracts, 35
Accelerated solvent extraction (ASE), 286 nutritional conditions, 35
Acetate pathway, 261 secondary metabolites, 12
Acetyl-CoA, 423 Alpha-pinene (C10H16), 289
Acids, 6 Alzheimer’s disease, 337
Adulteration of EOs Amplified fragment length polymorphism
analytical instruments, 263 (AFLP), 27
carvone/carvacrol, 265, 266 Animal sources, 150
cinnamaldehyde, 267 Antibacterial activity, EOs, 10
distillation technique, 270 Anti-inflammatory activity, EOs, 11
enantioselective testing, 270 Antimicrobial activity, EOs
health considerations, 269 betel leaf, 181–182
herbal medicines, 262 Antimicrobials
isotopic ratio testing, 270 and antibiotic resistance, 212
linalool and linalyl acetate, 264–266 bacteriocin sanguicin, 214
methyl salicylate, 267, 268 oral pathogens, 224, 228
natural essential oils, 270 S. sanguinis and S. salivarius, 217
naturally derived precursors, 270 strain types, 221, 223
nerolidol, 268, 269 Antioxidant activity, EOs
petrochemical-based synthetic products, 270 betel leaf, 180–181
precursor molecules, 270 Antioxidants, 11
substance/element, 262 antioxidant activity, EOs, 11
synthetic and natural compounds, 262 phenolic compounds, 10
synthetic markers (see Synthetic markers) Anti-termite activity, 247–248
therapeutic properties, 269 Anti-tick activity, 252
thymol, 266, 267 Applications, EOs
toxicity, 268 agricultural uses, 14
Alcohols, 6 aromatherapy, 14
Aldehydes, 6 industrial uses, 14, 15
Aldehydes dehydrogenase (ADH), 372 pharmacology and medicinal
Alecrim-de-vaqueiro, 242 uses, 13
Aliphatic 8-carbon compounds, 404 pines’ EOs
Allelochemicals, 35 industrial uses, 291
Allelopathy medicinal use, 290
allelochemicals, 12, 35 in veterinary medicine, 13–14
definition, 12, 35 Aroma-forming compounds, 404
pine Cross-talk
categorization, 287 MEP and MVA pathways, 315
shikimate, 288 terpene synthesis pathways, 311
terpenoids, 288, 289 Croton stellatopilosus, 376
terpenes, 5 Croton tetradenius, 244
volatile fraction, 5 Cypress oil, 367
Chinese herbal tradition, 262 Cytotoxicity, 12
Chiral GC-MS, 263, 270
Chromatographic techniques, 26
Cineol, 206 D
1,8-Cineole, 197, 199, 227 Dacryodes
Cinnamaldehyde, 267 antibacterial, 111
Cistus ladaniferus, 367 antioxidant, 111
Citronella oil, 13 antiplasmodial and anticarcinogenic
Civet, 151 properties, 111
Civetone, 150, 151 D. buettneri, 115, 117
Classical analytical techniques, 9 D. edulis, 115–117
Clevenger’s apparatus, 159, 160, 165 D. hexandra, 117
Clustered regularly interspaced short D. igaganga, 116
palindromic repeats D. klaineana, 117
(CRISPR), 304 D. rostrata, 117
Cold pressing, 7, 261 Dairy products, 403
Commiphora Dental plaque
C. africana, 99, 100 S. salivarius, 212
C. erythraea, 100 S. sanguinis, 212
C. gileadensis, 100, 101 Dentistry processing systems, 229
C. guidotii, 99 1-Deoxy-D-xylulose-5-phosphate (DXP),
C. guidottii, 101, 103 305, 307
C. habessinica, 103 Dimethylallyl diphosphate (DMADP), 372
C. holtziana ssp. holtziana, 103 Distillation, 9
C. kua, 103 Dry resin, 367
C. leptophloeos, 98, 104 Ducts, 359
C. molmol, 104 DXP reductoisomerase (DXR), 305, 307,
C. mukul, 99 310–311, 314, 319, 376
C. myrrha, 99, 105, 106 DXP synthase (DXS), 305, 307–311, 319,
C. ornifolia, 106 329, 339, 372, 376
C. parvifolia, 106
C. planifrons, 107
C. pyracanthoides, 107 E
C. socotrana, 107 Ecological factors, 401
C. tenuis, 107 Economic marketing, 359
myrrh and opopanax, 98 Economics, betal leaf, 156, 157, 185
traditional medicine Edible oils, 149
antitumoral usage, 108 Endodontic therapy, 212
aromatic applications, 108 Endo-perio syndrome, 216
assorted, 109 Enfleurage, 8
infections treatment, 108 Environmental conditions, 28
inflammatory conditions, 108 Enzyme technology, 359–361
myrrh, 108 Eplingiella fruticosa, 242, 243
oral usage, 108 Eremothecium ashbyi
wound treatment, 108 aromatic and monoterpene alcohol
Conidium formation, 412 accumulation, 417–425
Cosmetics, 366 asexual reproduction, 412, 414
CRISPR interference (CRISPRi), 321 biomass accumulation, 416
442 Index
Geranium oil I
components, 328 In vitro culturing, 373–376
phytochemical composition, 330–332 Insecticidal activity, 12
phytoconstituents, 328 anti-termite, 247–248
terpenes, 329 formicidal, 245–246
Geranyl diphosphate (GDP), 372 Green Revolution, 245
Geranylgeranyl diphosphate synthase plant-based products, 245
(GGPPS), 376 plant metabolites, 245
Ginger lily, 49 principal control method, 245
See also Hedychium spp. properties, 245
Gingivitis and gingival hypertrophy, 212 stored grain pests, 248–250
Glandular trichomes (GTs), 354, 357, 358 International Organization for Standardization
Gram-positive cocci and bacilli, 403 (ISO), 261
Green Revolution, 245 Isoflavones, 373
Greenhouse farming, 402 Isopentenyl diphosphate (IDP), 372, 410
Isoprene, 5
H
Hedychium essential oils K
antimicrobial and antioxidant activities, Ketones, 6
55–58 Kolbe-Schmitt reaction, 268
antimicrobial properties, 50
chemical constituents, 51–54
effectiveness, 55 L
fungal growth and aflatoxin Lactones, 7
secretion, 58 Lavandula genus, 301, 302
Gram-positive and -negative bacteria, 55 biosynthesis, EOs, 304–306
monoterpenes, 54, 55 commercial production, EOs, 302
oil constituents, 55 stated, mono- and sesquiterpenes, 304
oil extraction, plant parts use, 54 Lavandula latifolia
rhizomes, 54 in Arabidopsis and tobacco, 315
sesquiterpenes, 54, 55 chlorophyll and carotenoid content, 316
Hedychium spp. commercial production, EOs, 302
beneficial traits, 50 DXR, upregulation, 310
Indian populations, 55 DXS overexpression, 309
as ornamental plants, 50, 58 genetic transformation, 307
perfume essences, 49 HMGR, up-regulation, 311
rhizomes and aerial stems, 49 limonene, 315
“sweet” and “snow”, 49 pharmaceutical, cosmetic and food
uses, 49 industries, 319
Zingiberaceae family, 49 seedlings, 312
Hepatic encephalopathy, 215 upregulation, DXS, 309
Hydrocarbon, 5 Lavender EOs
Hydrodistillation (HD), 8, 261, 354 amount, chemical composition, and
Hydroperoxide lyases (HPL), 372 quality, 303
Hydrophobicity, 10 constituents, 303
1-Hydroxy-2-methyl-2-butenyl 4-diphosphate quality, 303
reductase (HDR), 376 Leaf-cutting ants, 243–246
3-Hydroxy-3-methylglutaryl CoA reductase Lemon balm, 239
(HMGR), 305, 307, 311, 315, Limonene, 197, 206, 289
319, 372 Linalool, 264–266, 303, 315, 318–320
Hypothetical metabolic model, 423, 424 Linalool synthase (LIS), 359
Hyptis pectinata, 243, 244 Linalyl acetate, 264–266
Index 445
R S
Random amplified polymorphic DNA Salivaricin E (SalE), 215
(RAPD), 27, 28 Sambaicatá, 243
Red flour beetle, 249 Santiria, 118
Repellent activity, 12 Seasonality, 36
Resin, 290 Secondary plant metabolites, 275
Riboflavinogenesis, 422 Selected ion monitoring analysis (SIM)
Root canal system, 216 method, 264
Rose-scented EOs Semi-synthetic methods, 366
alcohols and esters, 409 Sesquiterpene lactone, 197
biotechnology, 408 Sesquiterpenes, 20, 25, 28, 33, 54, 55, 71, 223,
components, 410 237, 241, 242, 264, 354, 372, 373
enzymatic reactions, 409 Sesquiterpenoids, 224, 229, 264, 365, 366,
and Eremothecium, 411 372, 373
floral-scented essential oil, 408 Shikimate, 288
isopentenyl diphosphate, 410 Shikimic acid pathway, 261
monoterpene alcohol, 410, 411 Simple sequence repeats (SSR), 27
mycelial fungi, 409 Single nucleotide polymorphisms (SNP), 27
phytogenous, 409 Site-specific natural isotope fractionation
quantity of aroma-forming compounds, 411 NMR (SNIF-NMR), 263
volatile fragrant compounds, 409 S-linalool, 359
yeast and yeast-like fungi, 409, 410 Solid media surface, 412
Rose-scented geranium Solvent extraction, 7, 8
economic status, 339–340 Solvent-free microwave extraction (SFME), 286
as flavoring agent, EOs, 327 Somatic embryogenesis, 344
genomic analysis, 338–339 Soxhlet extraction (SE), 284
glandular trichomes, 328 Spearman’s correlation coefficients, 424, 425
hydro-distillation method, 328 Specialised structures, 353, 354, 359, 361
448 Index
Specialized metabolism, 19, 20, 26, 29, 34–36 thymol, carvacrol,and p-cCymene, 227
Spike lavender univariate predictors, 222
environmental conditions, 304 Sub-lethal effect, 248
in natural environment, 302 Supercritical CO2 extraction, 8
perfumery and cosmetics, 303 Supercritical fluid extraction (SFE),
Steam distillation (SD), 9, 261, 354 284, 354
extraction process, 283, 284 Synthetic biology, 304
Stored grain pests, 248–250 Synthetic markers
Streptococcus salivarius, 213 adulteration detection, 263
clinical vs. referent ATCC strains, carvone/carvacrol, 266
220–222 cinnamaldehyde, 267
endocarditis, 215, 216 discovery, 269–270
EOs, 219, 220 linalool and linalyl acetate, 265, 266
infected dental root canal (oral focus), methyl salicylate, 268
216, 217 nitrogen-containing, 265
MIC values, 220 plinols, 265
pre-set criteria, EOs, 217 thymol, 267
thymol-rich oils, 220 toxicity, 268
Streptococcus sanguinis, 213 types, 264
anti-S. mutans and anti–
periodontopathogenic, anti-biofilm
role, 213–215 T
cineole-rich oils, 220 Taxonomic origin, 261
clinical vs. referent ATCC strains, Termites, 245, 247, 248
220–222 Terpenes, 237, 251, 291
endocarditis, 215, 216 alcohols, 6
EOs, 219, 220 classification, 5
infected dental root canal (oral focus), common formula, 329
216, 217 diterpenes, 5
pre-set criteria, EOs, 217 isoprene unit, 329
thymol-rich oils, 220 monoterpenes, 5
Streptococcus spp. properties, 5
antimicrobial activity, EOs, 222, 223 rose-scented geranium oil
ATCC 9222, 226 biosynthesis, 334
ATCC 10566, 225 cyclization, 335
camphor, 228 MVA pathway, 334
chemical class, 222, 223 pathway, 332, 333
chemical composition, 224 synthesis, diverse terpenes, 334
1,8-cineole, 227 sesquiterpene, 5
clinical isolate/referent strain, 224 Terpenoids, 288–290, 365, 372, 373,
efficacy, 226 376, 377
eugenol, 228 Terpineol (C10H18O), 289
logistic regression analysis, 222, 224 Thermolabile compounds, 354
monoterpene hydrocarbons, 223, 226, Thujone, 7, 198, 200, 203
227, 229 Thymol, 227, 266, 267, 270
monoterpenoids, 224, 226 Ticks, 253
myrcene, 228 Tissue culture, geranium
oxygenated monoterpenes, 224, 226, agricultural traits, 340
227, 229 cost, geranium seeds, 340
sesquiterpenes, 223 cultivation rate, Geranium, 340
sesquiterpenoids, 224 cutting, geranium, 340
strain types, 222 genetic transformation technology, 345
Index 449
in vitro organogenesis U
cotyledons, hypocotyls and root, Ultrasound-assisted extraction (UAE), 285, 286
342–343 Ultrasound-assisted hydro-distillation
leaf and petiole, 342 (UAHD), 163
regeneration using mature seeds, 343 Unsaturated fatty acids, 372
shoot tip, 342
meristem tip culture, 341
somaclonal variations, 343–344 V
somatic embryogenesis, 344 Vaporization, 25
Tissue/cell cultures, 374–375 Varronia curassavica, 241, 242
Toxic ketones, 7 Vegetative reproduction, 412
Trace analysis, 267–270 Volatile oils, 149
Traditional medicine, 262 Volatile-oil-bearing plants, 401, 430
Transgenic plant species, 378 Volatile-oil-bearing raw material, 408
Transgenic plants, 359, 360 Volatility, 365, 366
Trattinnickia, 118, 119
“Tree-betel vine” (Gaach Paan), 154
Trichoderma viride, 404 W
TrichOME database, 357, 358 World Health Organization (WHO), 262
Triterpenoids, 366, 379
Tryptophan decarboxylase (TDC), 360
Turbo distillation process, 9 Y
Tyrosine, 372 Yeast and yeast-like fungi, 409, 410