Operating Manual Photoflex WTW
Operating Manual Photoflex WTW
Operating Manual Photoflex WTW
pHotoFlex /
pHotoFlex Turb
Handheld photometer
ba75491d04 07/2006
Accuracy when The use of advanced technology and the high quality standard of our
going to press instruments are the result of continuous development. This may result
in differences between this operating manual and your instrument. Al-
so, we cannot guarantee that there are absolutely no errors in this man-
ual. Therefore, we are sure you will understand that we cannot accept
any legal claims resulting from the data, figures or descriptions.
1 Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.1 General features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.2 Keypad . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
1.3 Display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
1.4 Socket field . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
1.5 LabStation (optional) . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
2 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
2.1 Authorized use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
2.2 General safety instructions . . . . . . . . . . . . . . . . . . . . . . . 14
3 Commissioning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
3.1 Scope of delivery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
3.2 Power supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
3.3 Connecting the LabStation . . . . . . . . . . . . . . . . . . . . . . . 19
3.4 Initial commissioning . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
4 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
4.1 Switching on the meter . . . . . . . . . . . . . . . . . . . . . . . . . . 23
4.2 Inserting a cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
4.3 General operating principles . . . . . . . . . . . . . . . . . . . . . 27
4.3.1 Operating modes . . . . . . . . . . . . . . . . . . . . . . . . 27
4.3.2 Navigation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
4.3.3 Navigation example 1: Setting the language . . . 30
4.3.4 Navigation example 2: Setting the date and time 31
4.3.5 Menu overview . . . . . . . . . . . . . . . . . . . . . . . . . 33
4.4 System settings (System menu) . . . . . . . . . . . . . . . . . . 35
4.4.1 Measured value memory . . . . . . . . . . . . . . . . . . 36
4.4.2 Display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
4.4.3 Interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
4.4.4 Date/time . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
4.5 Photometry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
4.5.1 General information . . . . . . . . . . . . . . . . . . . . . . 40
4.5.2 Settings for photometric measurements . . . . . . 41
4.5.3 Measuring the concentration . . . . . . . . . . . . . . . 42
4.5.4 Blank value (reagent blank value) . . . . . . . . . . . 43
4.5.5 Measuring the absorbance/transmission . . . . . 45
4.5.6 Zero adjustment . . . . . . . . . . . . . . . . . . . . . . . . 46
4.5.7 Programs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
4.5.8 Analysis timer . . . . . . . . . . . . . . . . . . . . . . . . . . 49
4.5.9 Timer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
4.5.10 Measuring diluted samples . . . . . . . . . . . . . . . . 50
4.6 pH value / ORP voltage . . . . . . . . . . . . . . . . . . . . . . . . . 51
4.6.1 General information . . . . . . . . . . . . . . . . . . . . . . 51
4.6.2 Measuring the pH value . . . . . . . . . . . . . . . . . . 52
4.6.3 Measuring the ORP voltage . . . . . . . . . . . . . . . 53
4.6.4 Settings for pH and ORP measurements . . . . . 53
ba75491d04 07/2006 3
Contents pHotoFlex / pHotoFlex Turb
4.6.5 Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
4.6.6 Carrying out the TEC and NIST/DIN calibration
procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
4.7 Turbidity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
4.7.1 General information . . . . . . . . . . . . . . . . . . . . . . 62
4.7.2 Aligning and marking a cell . . . . . . . . . . . . . . . . 62
4.7.3 Measuring turbidity . . . . . . . . . . . . . . . . . . . . . . 63
4.7.4 Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
4.8 Storing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
4.8.1 Storing measurement datsets . . . . . . . . . . . . . . 69
4.8.2 Filtering measurement datsets . . . . . . . . . . . . . 70
4.8.3 Displaying measurement datsets . . . . . . . . . . . . 71
4.8.4 Downloading the measurement datsets to the
RS232
interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
4.8.5 Erasing stored measurement datasets . . . . . . . 72
4.9 Transmitting data (RS 232 interface) . . . . . . . . . . . . . . . 72
4.9.1 Connecting a PC/external printer . . . . . . . . . . . . 72
4.9.2 Configuring the RS232 interface . . . . . . . . . . . . 73
4.9.3 Selecting the output format of datasets . . . . . . . 73
4.9.4 Transmitting data . . . . . . . . . . . . . . . . . . . . . . . . 75
4.10 Reset . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
4.10.1 Resetting the system settings . . . . . . . . . . . . . . 76
4.10.2 Resetting the photometer settings . . . . . . . . . . . 77
4.10.3 Resetting the pH settings . . . . . . . . . . . . . . . . . . 77
4.11 Meter information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
4.12 Software update . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
4.13 Administrating user-defined methods . . . . . . . . . . . . . . . 79
4.13.1 Administrating user-defined programs with a
terminal program . . . . . . . . . . . . . . . . . . . . . . . . 80
6 What to do if... . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87
6.1 General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87
6.2 Photometry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88
6.3 pH value / ORP voltage . . . . . . . . . . . . . . . . . . . . . . . . . 88
6.4 Turbidity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90
7 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91
4 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Contents
8 Accessories, options . . . . . . . . . . . . . . . . . . . . . . . . . . . 95
8.1 WTW accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95
8.1.1 Connection cable: . . . . . . . . . . . . . . . . . . . . . . . 95
8.2 Optional extensions of the pHotoFlex (Turb) . . . . . . . . . 97
9 Lists . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99
10 Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105
ba75491d04 07/2006 5
Contents pHotoFlex / pHotoFlex Turb
6 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Overview
1 Overview
1.1 General features
The compact pHotoFlex (Turb) handheld precision photometer en-
ables you to carry out the following measurements quickly and reliably:
! Photometric measurements
– Concentration measurements
– Absorbance measurements
– Transmission measurements
! pH measurements
! Turbidity measurements (pHotoFlex Turb).
1 Keypad
2 Display
3 Cell shaft
(folded out for a 16 mm cell to be inserted)
4 Socket field
ba75491d04 07/2006 7
Overview pHotoFlex / pHotoFlex Turb
Note
If you need further information or application notes, you can obtain the
following material from WTW:
! Application reports
! Primers
! Safety datasheets.
1.2 Keypad
8 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Overview
Note
Keys with an additional number printed on are assigned doubly.
This enables to directly enter numbers in special menus. Thus, you
can, for example, conveniently enter the date and time via the number
keys.
ba75491d04 07/2006 9
Overview pHotoFlex / pHotoFlex Turb
1.3 Display
The graphic display shows all information of the current measurement
in the measured value display. The illumination enables to read the dis-
play even in the darkness.
1 2 3 4 5
10 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Overview
LabStation
ba75491d04 07/2006 11
Overview pHotoFlex / pHotoFlex Turb
12 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Safety
2 Safety
This operating manual contains basic instructions that you must follow
during the commissioning, operation and maintenance of the meter.
Consequently, all responsible personnel must read this operating man-
ual carefully before working with the meter. The operating manual must
always be available within the vicinity of the meter.
Target group The meter was developed for work in the field and in the laboratory.
We assume that, as a result of their professional training and experi-
ence, the operators will know the necessary safety precautions to take
when handling the chemicals of photometric test sets.
Safety instructions The individual chapters of this operating manual use the following safe-
ty instructions to indicate various types of danger:
Caution
indicates instructions that must be followed precisely in order to
avoid the possibility of slight injuries or damage to the instrument
or the environment.
Further notes
Note
indicates notes that draw your attention to special features.
Note
indicates cross-references to other documents, e.g. operating manu-
als.
ba75491d04 07/2006 13
Safety pHotoFlex / pHotoFlex Turb
Function and operating The smooth functioning and operational safety of the meter can only be
safety guaranteed if the generally applicable safety measures and the specific
safety instructions in this operating manual are followed during opera-
tion.
14 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Safety
The smooth functioning and operational safety of the meter can only be
guaranteed under the environmental conditions that are specified in
chapter 7 TECHNICAL DATA.
Safe operation It is the responsibility of the operator to continuously observe the over-
all technical condition (externally recognizable deficits and damage as
well as alterations to the operational behavior) of the meter.
If you are in any doubt, please contact the supplier of the instrument.
Caution
Danger of eye damage by visible and invisible LED radiation. In
the cell shaft there are light emitting diodes (LED) of the 1M class.
Do not look at the radiation using optical instruments.
With normal, authorized use there is no hazard.
Obligations of the The purchaser of this meter must ensure that the following laws and
operator guidelines are observed when using dangerous substances:
! EEC directives for protective labor legislation
! National protective labor legislation
! Safety regulations
! Safety datasheets of the chemical manufacturers.
ba75491d04 07/2006 15
Safety pHotoFlex / pHotoFlex Turb
16 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Commissioning
3 Commissioning
3.1 Scope of delivery
! Handheld photometer, pHotoFlex Turb or pHotoFlex
! 4 batteries, 1.5 V type AA (in the battery compartment)
! Optional: Accumulator pack and power pack with Euro plug
and exchange plugs for USA, UK, and Australia
! Optional: LabStation
! 1 empty cell 16 mm
! 2 empty cells 28 mm
with label to mark the cell for turbidity measurements
! AMCO®-Clear turbidity standard (pHotoFlex Turb only)
! Microfiber cloth to clean the meter
! Compact operating manual and short operating manual
! CD-ROM with
– detailed operating manual
– photometry analysis manual with analysis specifications
– software to program user-defined methods
Note
The optional parts of the scope of delivery are available as accessories
(see section 8.1).
Caution
ba75491d04 07/2006 17
Commissioning pHotoFlex / pHotoFlex Turb
Note
The accumulator pack should not be completely discharged. If you do
not operate the instrument for a longer period of time you should charge
the accumulator pack every six months.
Automatic switchoff The meter has an automatic switch-off function in order to save the bat-
teries or accumulator pack (see section 4.4).
Display illumination During operation with batteries or accumulator pack the meter automat-
ically switches off the display illumination if no key is pressed for
30 seconds. The illumination is switched on with the next keystroke
again. The display illumination can also be switched off completely (see
section 4.4.2).
Note
Power pack and accumulator pack are available as an accessory (see
section 8.1).
2 3
1 If necessary, replace the Euro plug (1) on the power pack (2)
by the country-specific plug suitable for your country.
2 Connect the plug (3) to the socket (4) of the photometer.
3 Connect the power pack to an easily accessible power socket.
18 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Commissioning
In order to use the functions of the LabStation for operation in the lab-
oratory, connect the LabStation and place the pHotoFlex (Turb) in the
LabStation.
5
4
2 3
1 If necessary, replace the Euro plug (1) on the power pack (2)
by the country-specific plug suitable for your country.
2 Connect the plug (3) to the socket (4) of the LabStation.
3 Connect a PC, printer or barcode reader to the socket (5) of the
LabStation as necessary.
Note
When the pHotoFlex (Turb) is operated in the LabStation, a barcode
reader can be connected. A barcode reader enables to select program
numbers in a simplified manner.
Recommended barcode readers can be found in section 8.2.
For the interface of your meter, set the same baud rate that is set for
the barcode reader (see section 4.4.3).
The baud rate of the barcode reader is given in the operating manual
of your barcode reader.
ba75491d04 07/2006 19
Commissioning pHotoFlex / pHotoFlex Turb
LabStation
20 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Commissioning
! For
– accumulator operation: insert the accumulator pack (see
section 5.1.2)
– line power operation and charging the accumulator pack: connect
the power pack (see section 3.2)
– operation with LabStation: connect the LabStation and place the
pHotoFlex (Turb) in the LabStation (see section 3.3)
! Switch on the meter (see section 4.1)
! Set the language as necessary (see section 4.3.3)
! Set the date and time as necessary (see section 4.3.4)
Note
When you set the language, date and time according to the mentioned
sections of this operating manual you will quickly become familiar with
the simple operation of the pHotoFlex (Turb).
ba75491d04 07/2006 21
Commissioning pHotoFlex / pHotoFlex Turb
22 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Operation
4 Operation
4.1 Switching on the meter
Switching on Press the <ON/OFF> key.
For 30 seconds, Start menu appears with a selection of the measuring
modes. The measuring mode last selected is highlighted.
The status line indicates the meter designation and the version number
of the software.
Start
Photometry
Turbidity
pH & ORP
i pHotoFlex V 0.24
Photometry \ Concentration
i Select program
with <PROG>
01.02.05 15:12
Automatic switchoff The meter has an automatic switchoff function in order to save the bat-
teries or accumulator pack (see section 4.4). The automatic switchoff
switches the meter off if no key is pressed for an adjustable period.
ba75491d04 07/2006 23
Operation pHotoFlex / pHotoFlex Turb
Display illumination During operation with batteries or accumulator pack the meter automat-
with battery and accu- ically switches off the display illumination if no key is pressed for
mulator pack operation 30 seconds. The illumination is switched on again with the next key-
stroke.
Inserting a 28 mm cell
2 Insert the cell so that it is positioned on the bottom of the cell
shaft.
The cell is ready to be measured.
24 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Operation
Inserting a 16 mm cell
1 Put the fold-out cell shaft (2) in an upright position until it locks
into place.
ba75491d04 07/2006 25
Operation pHotoFlex / pHotoFlex Turb
Note
For optimum measurement results, the cell must always be covered by
the external light cover. Otherwise, external light can falsify the mea-
surement result.
26 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Operation
Operating elements, An overview of the operating elements and the display is given in
display section 1.2 and section 1.3.
Operating modes, An overview of the operating modes of the pHotoFlex (Turb) and the
navigation navigation through menus and functions can be found in section 4.3.1
and section 4.3.2.
4.3.2 Navigation
ba75491d04 07/2006 27
Operation pHotoFlex / pHotoFlex Turb
Menus and dialogs The menus for settings and dialogs in courses contain further sub-
menus. The selection is made with the <▲> <▼> keys.
The current selection is displayed in reverse video.
! Menus
The name of the menu is displayed at the upper edge of the frame.
Menus are opened by confirming with <START/ENTER>. Example:
Configuration
Photometry
Turbidity
pH & ORP
System
Info
! Settings
Settings are indicated by a colon. The current setting is displayed on
the right-hand side. With <START/ENTER>, the selection of the
possible settings is opened. Subsequently, the setting can be
changed with <▲> <▼> and <START/ENTER>.
Example:
System
Language: Deutsch
Beep: Off
Illumination: On
Contrast: 48 %
Temperature unit: °C
Switchoff time: 30 min
! Functions
Functions are designated by the name of the function. They are im-
mediately carried out by confirming with <START/ENTER>.
Example: display the Calibration record function
(in the pH & ORP / Calibration menu).
pH & ORP
Calibration record
Cal. type: AutoCal
TEC
Calibration interval: 007 d
Unit for slope: mV/pH
:i 2.00 4.01 7.00 10.01
! Messages
Information or instructions are marked by the i symbol. They can-
not be selected.
28 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Operation
Example:
pH & ORP
i Buffer recognition TEC The i symbol indicates
i Immerse sensor in buffer 1 info texts, e.g.
messages, notes or instruc-
tions
Set temperature: 25 °C
Continue
Note
The principles of navigation are explained in the two following sections
by reference of examples:
! Setting the language (section 4.3.3)
! Setting the date and time (section 4.3.4).
ba75491d04 07/2006 29
Operation pHotoFlex / pHotoFlex Turb
Note
The following example describes in the language of the country how to
set the language. On delivery, English is set as the language in the
pHotoFlex (Turb). During initial commissioning, the language is set in
the menu, Configuration / System / Language.
System
Language: Deutsch
Store
Display
Reset
Interface
Continue ...
System
Language: Deutsch
Store
Display
Reset
Interface
Continue ...
30 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Operation
The meter has a clock with a date function. The date and time are indi-
cated in the status line of the measured value display. When storing
measured values and calibrating, the current date and time are auto-
matically stored as well.
The correct setting of the date and time and date format is important for
the following functions and displays:
! Current date and time
! Calibration date
! Identification of stored measured values.
Note
After a fall of the supply voltage (empty batteries or accumulator pack),
the date and time are reset to 01.01.2003, 00:00 hours.
Setting the date, time The data format can be switched from the display of day, month, year
and date format (dd.mm.yy) to the display of month, day, year (mm/dd/yy or mm.dd.yy).
Date/time
Time: 14:53:40
Date: 30.10.03
Date format: dd.mm.yy
ba75491d04 07/2006 31
Operation pHotoFlex / pHotoFlex Turb
3 Select and confirm the Time menu with <▲> <▼> and
<START/ENTER>.
A display for the entry of numerals with the number keys opens
up.
Time
14:53:40
Note
In the case of wrong entries, you can cancel the procedure with <ESC>.
After canceling with <ESC>, it is possible to enter all digits once again.
The new digits are only taken over by confirming with <START/EN-
TER>.
32 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Operation
ba75491d04 07/2006 33
Operation pHotoFlex / pHotoFlex Turb
34 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Operation
ba75491d04 07/2006 35
Operation pHotoFlex / pHotoFlex Turb
In the Measured value memory menu, you find functions to display and
edit the stored measurement datasets:
! Display the measurement datsets on the screen (Display)
! Download the measurement datsets to the RS232 interface (RS232
download)
! Set up filter rules for the stored measurement datsets (Data filter)
! Erase all stored measurement datsets (Delete)
! Information on the number of occupied memory locations
36 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Operation
Note:
All calibration data remains
stored when performing this
action.
ba75491d04 07/2006 37
Operation pHotoFlex / pHotoFlex Turb
4.4.2 Display
In the Configuration / System / Display menu, you set the display fea-
tures:
! Switching on/off the display illumination (Illumination)
! Display contrast (Contrast)
4.4.3 Interface
38 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Operation
4.4.4 Date/time
ba75491d04 07/2006 39
Operation pHotoFlex / pHotoFlex Turb
4.5 Photometry
Note
Suitable analysis specifications for test sets can be found in the pho-
tometry analysis manual (on CD-ROM).
There you will also find further instructions on handling chemicals and
on how to proceed when applying the analysis specifications.
Methods and the corresponding method data for many test sets are
stored as programs in the pHotoFlex (Turb). A program number is as-
signed to each program.
You can measure the following parameters with the pHotoFlex (Turb):
! Concentration [mg/l]
! % Transmission []
! Absorbance []
40 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Operation
Preparatory activities Perform the following preparatory activities when you want to measure:
1 Clean the cells before filling them with sample and also before
measuring as necessary (see section 5.2.2).
The cells must be absolutely clean and free of scratches.
2 For measurement, place the pHotoFlex (Turb) on a horizontal
surface.
ba75491d04 07/2006 41
Operation pHotoFlex / pHotoFlex Turb
1 Press the <M> key (long pressure) repeatedly until the Pho-
tometry measuring mode is selected.
2 Press the <M> key (short pressure) repeatedly until the mea-
sured variable, Konzentration is selected.
Note
From the second concentration measurement, the data of the program
last used is automatically displayed here.
With <▲> <▼> you can quickly switch between the ten programs last
used.
Note
If a program number is selected that requires a measured blank value,
the menu automatically guides to the blank value measurement.
42 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Operation
Photometry \ Concentration
i Insert sample
i Start measurement
with <START>
1: A5/25 MC NH4-N
16 mm 0.20 - 6.51 mg/l
01.02.05 15:12
Photometry \ Concentration
[BV] A blank value measured by
the user is used
0.74 mg/l
1: A5/25 MC NH4-N
16 mm 0.20 - 6.51 mg/l
01.02.05 15:12
Note
You will find more information on blank values in the photometry anal-
ysis manual.
A blank value is always stored for the program that has just been called
up. It remains stored until it is erased (menu item, Delete blank value)
or overwritten.
The Reset function erases all blank values measured by the user and
restores the blank values stored in the factory.
If a blank value measured by the user is stored for a program, this blank
value is used for measurement. The usage of the blank value mea-
sured by the user is documented and also indicated in the measured
value display.
ba75491d04 07/2006 43
Operation pHotoFlex / pHotoFlex Turb
Measuring the blank 1 Press the <M> key (long pressure) repeatedly until the Pho-
value tometry measuring mode is selected.
2 Press the <M> key (short pressure) repeatedly until the mea-
sured variable, Concentration is selected.
3 Select a program with <PROG> as necessary.
Note
The following measurement of the blank value applies only to the se-
lected program. If no program is selected, the message i No program
selected. appears on the display.
Photometry \ Adjustment
Zero adjust.
Measure blank value
Delete blank value
Start measurement
44 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Operation
Note
The transmission measurement is not described separately in the fol-
lowing example as it operates in exactly the same way as the absor-
bance measurement. The result of the measurement is displayed in %
Transmission.
1 Press the <M> key (long pressure) repeatedly until the Pho-
tometry measuring mode is selected.
2 Press the <M> key (short pressure) repeatedly until the mea-
sured variable, Absorbance or % Transmission is selected.
Photometry \ Absorbance
i Select cell
with ▲ ▼
16 mm 610 nm
01.02.05 15:12
3 Select the cell diameter with <▲> <▼> and confirm with
<START/ENTER>.
4 Select the wavelength with <▲> <▼> and confirm with
<START/ENTER>.
ba75491d04 07/2006 45
Operation pHotoFlex / pHotoFlex Turb
Photometry \ Absorbance
i Insert sample
i Start measurement
with <START>
16 mm 610 nm
01.02.05 15:12
Photometry \ Absorbance
0.532
16 mm 610 nm
01.02.05 15:12
1 Press the <M> key (long pressure) repeatedly until the Pho-
tometry measuring mode is selected.
2 Press the <M> key (short pressure) repeatedly until the mea-
sured variable, Concentration is selected.
3 Press the <CAL/ZERO> key.
The menu for adjustment measurements opens up.
46 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Operation
Photometry \ Adjustment
Zero adjust.
Measure blank value
Delete blank value
Zero adjust.
i Insert zero
cell (dist. water)
Cell 16 mm
Start measurement
Note
If i Calibration error! was displayed as the calibration result, a note au-
tomatically reminds you of another zero adjustment before the next
measurement.
Measuring is not possible without a valid zero adjustment.
ba75491d04 07/2006 47
Operation pHotoFlex / pHotoFlex Turb
4.5.7 Programs
Displaying program You can view the most important data of all methods.
data The method data is ordered according to the program number.
Programs
1: A5/25 MC Program number, test, code
0.20 - 8.00 mg/l
Measuring range with unit
NH4-N
16 mm Citation form
Note
This data is also to be found in the photometry analysis manual in the
overview of the test sets and in the individual analysis specifications for
the test sets.
Updating programs Under http://www.WTW.com on the Internet, you can always find the
latest software version with the newest programs and method data for
your pHotoFlex (Turb) (see APPENDIX: FIRMWARE UPDATE).
User-defined programs User-defined programs (methods) can be stored under program num-
bers between 900 and 999. You can store up to 100 user-defined pro-
grams (see section 4.13).
48 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Operation
If you want to manually enter time intervals, use the Timer function (see
section 4.5.9).
The Analysis timer with the required time interval is automatically dis-
played at the due point.
Start the Analysis timer with the <START/ENTER> key.
It is not possible to shorten the time intervall.
An acoustic signal sounds when the adjusted time interval has expired.
4.5.9 Timer
ba75491d04 07/2006 49
Operation pHotoFlex / pHotoFlex Turb
After entering the factor for the dilution the meter converts the concen-
tration to that of the undiluted sample.
The display then indicates the measured value of the undiluted sample.
Entering the factor of 1 Select the program for which a dilution factor is to be entered.
the dilution
2 Open the Configuration / Photometry / Dilution menu.
The current factor of the dilution is displayed.
Dilution
Water + sample 0+1
i PROG 1
The entered dilution factor is only valid for the selected program. The
dilution factor is erased if:
! the photometer is switched off
! a different program number is selected
! the factor 0 is entered in the Dilution menu.
50 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Operation
Caution
When connecting an earthed PC/printer, measurements cannot be
performed in earthed media as incorrect values would result. The
RS232 interface is not galvanically isolated.
Preparatory activities Perform the following preparatory activities when you want to measure:
ba75491d04 07/2006 51
Operation pHotoFlex / pHotoFlex Turb
pH & ORP \ pH
6.94
25.0 °C
[AR]
01.02.05 15:12
3 Press the <M> key (short pressure) repeatedly until the mea-
sured variable, pH is selected.
AutoRead The AutoRead function (drift control) continually checks the stability of
(Drift control) the measurement signal. The stability has a considerable impact on the
reproducibility of measured values. The display of the measured pa-
rameter flashes until a stable measured value is available.
52 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Operation
Note
ORP electrodes are not calibrated. However, you can check ORP elec-
trodes using a test solution.
157 mV
25.0 °C
[AR]
01.02.05 15:12
3 Press the <M> key (short pressure) repeatedly until the mea-
sured parameter, ORP is selected.
AutoRead The AutoRead function (drift control) continually checks the stability of
(drift control) the measurement signal. The stability has a considerable impact on the
reproducibility of measured values. The display of the measured pa-
rameter flashes until a stable measured value is available.
Overview For pH and ORP measurements, the following settings are available in
the Configuration / pH & ORP menu:
! Measured parameter
! Calibration record (display, print)
! Selecting the calibration type
! Entering the Calibration interval
! Selecting the Unit for slope
! Selecting the Temperature unit
! Reset
ba75491d04 07/2006 53
Operation pHotoFlex / pHotoFlex Turb
Settings/functions The settings can be found in the Configuration / pH & ORP menu.
To switch to the Configuration menu, press the <MENU> key.
54 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Operation
4.6.5 Calibration
Why calibrate? pH electrodes age. This changes the asymmetry (zero point) and slope
of the pH electrode. As a result, an inexact measured value is dis-
played. Calibration determines the current values of the asymmetry
and slope of the electrode and stores them in the meter. Thus, you
should calibrate at regular intervals.
Buffer sets for You can use the buffer sets quoted in the table for an automatic calibra-
calibration tion. The pH values are valid for the specified temperature values. The
temperature dependence of the pH values is taken into account during
calibration.
Note
The buffers are selected in the Configuration / pH & ORP / Cal. type
menu, see section 4.6.4).
ba75491d04 07/2006 55
Operation pHotoFlex / pHotoFlex Turb
Calibration points Calibration can be performed using one, two or three buffer solutions in
any order (single-point-, two-point or three-point calibration). The meter
determines the following values and calculates the calibration line as
follows:
Note
You can display the slope in the unit, mV/pH or % (see section 4.6.4).
Calibration record When finishing a calibration, the new calibration values are displayed
as an informative message (i symbol) first. Then you can decide
whether you want to take over these values of the new calibration or
whether you want to continue measuring with the old calibration data.
After accepting the new calibration values the calibration record is dis-
played.
Displaying and down- You can view the data of the last calibration on the display. Subse-
loading calibration data quently, you can download the displayed calibration data to the inter-
to interface face, e. g. to a printer or PC, with the <PRT> key.
The calibration record of the last calibration can be found under the
Configuration / pH & ORP / Calibration / Calibration record menu item.
56 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Operation
Sample printout of a
31.10.03 16:13
record pHotoFlex Ser. no. 12345678
Calibration pH & ORP
Calibration date 31.10.03 16:13:33
Calibration interval 7 d
AutoCal TEC
Buffer 1 4.01
Buffer 2 7.00
Buffer 3 10.01
Voltage 1 184.0 mV 24.0 °C
Voltage 2 3.0 mV 24.0 °C
Voltage 3 -177.0 mV 24.0 °C
Slope -60.2 mV/pH
Asymmetry 4.0 mV
Sensor +++
Calibration evaluation After calibrating, the meter automatically evaluates the calibration. The
asymmetry and slope are evaluated separately. The worse evaluation
of both is taken into account. The evaluation appears on the display
and in the calibration record.
ba75491d04 07/2006 57
Operation pHotoFlex / pHotoFlex Turb
Preparatory activities Perform the following preparatory activities when you want to calibrate:
The two calibration procedures only differ in the usage of different buff-
er sets (see section 4.6.5). Make sure the Cal. type is correctly set in
the pH & ORP / Calibration menu (see section 4.6.4).
For this procedure, use any one, two or three WTW technical buffer so-
lutions in ascending or descending order.
Note
The TEC calibration for pH 10.01 is optimized for the WTW technical
buffer solution TEP 10 Trace or TPL 10 Trace. Other buffer solutions
can lead to an erroneous calibration. The correct buffer solutions are
given in the WTW catalog or on the Internet.
1 Press the <M> key (short pressure) repeatedly until the mea-
sured parameter, pH or ORP is selected.
2 Start the calibration with <CAL/ZERO>.
The calibration display appears.
Continue
58 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Operation
Terminate AutoRead
Note
For single-point calibration, the instrument uses the Nernst slope
(-59.16 mV/pH at 25 °C) and determines the asymmetry of the elec-
trode.
ba75491d04 07/2006 59
Operation pHotoFlex / pHotoFlex Turb
Terminate AutoRead
60 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Operation
Terminate AutoRead
ba75491d04 07/2006 61
Operation pHotoFlex / pHotoFlex Turb
4.7 Turbidity
Venting the sample Air bubbles in the sample affect the measuring result to a massive ex-
tent because they have a large scattering effect on the incident light.
Larger air bubbles cause sudden changes in the measured values
whereas smaller air bubbles are recorded by the instrument as turbidi-
ty. Therefore, avoid or remove air bubbles:
Avoiding or removing ! During sampling, ensure all movement is kept to a minimum
air bubbles ! If necessary, vent the sample (ultrasonic baths, heating or adding a
surface-active substance to reduce the surface tension)
Aligning the cell 1 Press the <M> key (long pressure) repeatedly until the Turbid-
ity measuring mode is selected.
2 Clean the cell (see section 5.2.2).
3 Insert the cell (see section 4.2).
4 Align the cell:
! Press and hold the <START/ENTER> key.
! Slowly and in small steps turn the cell by one complete ro-
tation (by 360 °).
After each step wait for a short time until the displayed mea-
sured value is stable.
! Turn the cell back to the position with the lowest measured
value.
Note
To keep the drift as low as possible, the time for aligning the cell while
pressing and holding the <START/ENTER> key is limited to 30 sec-
onds. After this time, the meter starts measuring automatically.
62 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Operation
The marking can, e. g., be done on a label on the cap of the cell.
Caution
Never pour any liquids directly into the cell shaft. Always use a
cell for measurement. The meter only measures precisely if the
cell is closed with the black light protection cap (WTW cells).
Note
The outside of the cell always has to be clean, dry, and free of finger-
prints and scratches. Clean the cell before starting to measure (see
section 5.2.2). Only hold the cells by the top or by the black light pro-
tection cap.
Measuring 1 Press the <M> key (long pressure) repeatedly until the Turbid-
ity measuring mode is selected.
2 Rinse out a clean cell with the sample to be measured:
Pour approximately 10 ml sample into the cell. Close the cell
and rotate it several times before throwing the sample away.
3 Repeat the rinsing procedure twice more.
4 Fill the cell with the sample to be measured (approx. 15 ml).
Close the cell with the black light protection cap.
5 Clean the cell (see section 5.2.2).
6 Insert the cell (see section 4.2).
ba75491d04 07/2006 63
Operation pHotoFlex / pHotoFlex Turb
Note
To keep the drift as low as possible, the time for aligning the cell while
pressing and holding the <START/ENTER> key is limited to 30 sec-
onds. After this time, the meter automatically starts measuring or cali-
brating.
Turbidity
FNU
157.0 NTU
01.02.05 15:12
Display if the If the measured value is outside the measuring range of the
measuring range is pHotoFlex Turb, it is indicated on the display:
exceeded
Turbidity
FNU
> 1100 NTU
01.02.05 15:12
64 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Operation
4.7.4 Calibration
Calibration procedures For the menu-guided three-point calibration you need the following
and calibration three calibration standards in the mentioned order:
standards
Standard no. NTU/FNU
1 1000
2 10,0
3 0,02
Preparing calibration Perform the following preparatory activities when you want to calibrate:
Caution
Never open the cells with the calibration standards.
Carrying out calibration 1 Press the <M> key (long pressure) repeatedly until the Turbid-
ity measuring mode is selected.
2 Press the <CAL/ZERO> key.
The menu-guided calibration begins.
Follow the instructions on the display.
Turbidity \ Calibration
i Insert standard
1000 FNU/NTU
i Press and hold <START>
i Align sample
3 Insert the cell with the displayed calibration standard (here e.g.
1000 NTU/FNU) in the cell shaft (see section 4.2).
ba75491d04 07/2006 65
Operation pHotoFlex / pHotoFlex Turb
Turbidity \ Calibration
i Turb. = 1000 FNU/NTU
i Start calibration
by releasing <START>
Note
Before measuring the third calibration standard of 0.02 NTU/FNU you
can exit the calibration with <ESC> at any time.
The new calibration data is discarded. The old calibration data is further
used.
66 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Operation
Turbidity
i Insert sample
i Press and hold <START>
i Align sample
Note
If i Calibration error! was displayed as the calibration result, a note ap-
pears on the display to recalibrate before measuring.
Should a valid calibration not be possible the meter also offers to con-
tinue measuring with the last valid calibration data.
ba75491d04 07/2006 67
Operation pHotoFlex / pHotoFlex Turb
4.8 Storing
The meter has 1000 storage locations for measurement datsets.
You can transmit measured values (datasets) to the data storage with
the <STO> key.
Each storage process transmits the current dataset to the interface at
the same time.
The number of storage locations that are still free is displayed in the
Store menu. The number of storage locations that are occupied is dis-
played in the System \ Measured value memory menu.
68 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Operation
ID: 1
Store (ID: 1:
Note
A measurement dataset is stored quickly by twice pressing <STO>. It
is stored with the ID last set.
If the storage is full You can erase the entire storage (see section 4.8.5), or overwrite the
oldest dataset with the next storing procedure.
A security prompt appears before a dataset is overwritten.
ba75491d04 07/2006 69
Operation pHotoFlex / pHotoFlex Turb
70 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Operation
You can read out stored datasets on the display. Only those datasets
are displayed that correspond to the selected filter criteria (see
section 4.8.2).
Start reading out the data on the display in the menu, Configuration /
System / Measured value memory / Display.
Display of a dataset
02.02.2005 11:24:16
ID: 1
7.000 (pH) 24.8 °C AR
i Scroll with ▲ ▼
Further datasets that correspond to the filter criteria are displayed with
the <▲> <▼> keys.
Quitting the display To quit the display of stored measurement datasets, you have the fol-
lowing options:
! Switch directly to the measured value display with <M> (short pres-
sure).
! Leave the display and switch to the higher menu with <ESC> or
<START/ENTER>.
You can download stored datasets to the RS232 interface. Only those
datasets are downloaded that correspond to the selected filter criteria
(see section 4.8.2).
ba75491d04 07/2006 71
Operation pHotoFlex / pHotoFlex Turb
Note
Erasing individual datasets is not possible. If all storage locations are
occupied, however, it is possible to overwrite the oldest dataset at a
time. A security prompt appears before a dataset is overwritten.
Connect the interface to the devices via the AK540/B (PC) or AK540/S
(external printer) cable.
Caution
The RS232 interface is not galvanically isolated.
When connecting an earthed PC/printer, measurements cannot be
performed in earthed media as incorrect values would result.
72 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Operation
You can set the following values for the baud rate on the
pHotoFlex (Turb) : 1200, 2400, 4800, 9600, 19200.
For downloading data to the interface you can select the output format.
etc...
ba75491d04 07/2006 73
Operation pHotoFlex / pHotoFlex Turb
15.01.05;15:06:49;1;8;O2;[BW];[9 + 1];6.5;mmol/l;VALID;
15.01.05;15:09:52;0;;;[AR];;7.00;<pH>;VALID;25;°C;VALID
11 12 13
Data Description
1 Date Date of storing
2 Time Time of storing
3 ID adjusted ID
4 Program number only for the measured parameter,
Concentration
5 Citation form only for the measured parameter,
Concentration
6 AR/ ! AutoRead (measuring mode, pH & ORP)
BV ! Blank value (measured parameter,
Concentration)
7 Dilution only for the measured parameter,
Concentration
8 Measured value ! Measured value or
! Upper/lower measuring range limit
(only with measured value status, OFL/
UFL)
9 Unit of 8 ! Unit of the measured value or
! Designation of dimensionless measured
values, e.g. <pH>
10 Measured value ! VALID: Measured value valid
status of 8 ! INVALID: Measured value invalid
! UFL: Measured value below the lower
measuring range limit
! OFL: Measured value above the upper
measuring range limit
11 Temperature val- ! Temperature (measuring mode, pH &
ue / wavelength ORP)
of the measure- ! Wavelength (measured parameter,
ment Absorbance / % Transmission)
74 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Operation
Data Description
12 Unit of 11 ! °C / °F
! nm
13 Measured value VALID, INVALID, UFL, OFL
status of 11
The following table shows which data are transmitted to the interface in
which way:
Note
With the <PRT> key you output the data that is being shown on the dis-
play to the interface (displayed measured values, stored measurement
datsets, calibration record).
ba75491d04 07/2006 75
Operation pHotoFlex / pHotoFlex Turb
4.10 Reset
You can reset (initialize) all system and measurement settings.
Note
For turbidity measurement, there are no resettable settings.
With the System / Reset function, all resettable settings are reset.
76 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Operation
With the Photometry / Reset function, all photometer settings are reset.
Note
The calibration data are reset to the default settings together with the
measuring parameters. Recalibrate after performing a reset.
ba75491d04 07/2006 77
Operation pHotoFlex / pHotoFlex Turb
Info
i Model: pHotoFlex
i Software: V 0.35
i Prog.: V 0.07
i Ser. no.: 04280003
The proceeding for updating the software can be found in the appendix
(see APPENDIX: FIRMWARE UPDATE).
78 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Operation
! entered
! read out
! erased.
Administration of the method data can be carried out in the LSdata soft-
ware (see operating manual LSdata) or with the aid of a terminal pro-
gram (section 4.13.1).
ba75491d04 07/2006 79
Operation pHotoFlex / pHotoFlex Turb
Note
The CD-ROM contains a configuration file for the HyperTerminal pro-
gram. By double-clicking the configuration file (*.ht), the HyperTerminal
with the required pre-settings for data exchange with the meter is
opened.
The pHotoFlex (Turb) can administrate the database of user-defined
programs via command lines.
Storing user-defined Enter a command line according to the following scheme in the terminal
programs program:
U.500#11,Program No,Model,Cuvette [mm],Wave length [nm],MRB,
MRE,E0,Slope,Resolution,Formula,Unit
Example:
U.500#11,900,Test,16,436,0.0,2.0,0.0,1.0,0.01,test,
mg/l
80 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Operation
Note
If a program is already stored under the selected number, the program-
ming procedure is canceled. To store the program under the selected
number, first erase the program stored under the number.
Reading out user- To read out user-defined programs, enter a command line according to
defined programs the following scheme in the terminal program:
ba75491d04 07/2006 81
Operation pHotoFlex / pHotoFlex Turb
82 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Maintenance, cleaning, disposal
Caution
Make sure that the poles of the batteries are the right way round.
The ± signs on the batteries must correspond to the ± signs in the
battery compartment.
ba75491d04 07/2006 83
Maintenance, cleaning, disposal pHotoFlex / pHotoFlex Turb
Caution
Use original WTW accumulator packs only.
84 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Maintenance, cleaning, disposal
5.2 Cleaning
Occasionally wipe the outside of the meter with a damp, lint-free cloth.
Disinfect the housing with isopropanol as required.
Caution
The housing components are made out of synthetic materials
(polyurethane, ABS and PMMA). Thus, avoid contact with acetone
and similar detergents that contain solvents. Remove any splash-
es immediately.
If liquid is in the cell shaft (e.g. due to a spilled cell), clean the cell shaft
as follows:
Caution
Cells can contain poisonous or corrosive substances. If the con-
tent is released follow the danger warnings on the cell. If neces-
sary, take corresponding protective measures (protective
goggles, protective gloves etc.).
1 Switch the pHotoFlex (Turb) off and pull out the power plug.
2 Rinse the cell shaft with distilled water.
1 Clean the cells inside and out with hydrochloric acid or labora-
tory soap.
2 Rinse out several times with distilled water.
3 Let them dry in the air.
4 Only hold the cells by the top or by the light protection cap so
that the optical path is not impaired.
5 Before measuring, clean the cell with the enclosed cleaning
cloth.
Note
Scratches in the glass change the optical characteristics of the cell and
falsify the measured value. For this reason, never use scratched cells!
ba75491d04 07/2006 85
Maintenance, cleaning, disposal pHotoFlex / pHotoFlex Turb
5.3 Disposal
Packing This meter is sent out in a protective transport packing.
We recommend: Keep the packing material. The original packing pro-
tects the meter from transport damages.
Batteries/accumulator Remove the batteries or accumulator pack from the meter (see
pack section 5.1).
Dispose of the batteries or accumulator pack at a suitable facility ac-
cording to local legal requirements. It is illegal to dispose of the accu-
mulator pack with household refuse.
NiMH
Meter Dispose of the meter without the batteries and accumulator pack as
electronic waste at an appropriate collection point.
86 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb What to do if...
6 What to do if...
6.1 General errors
ba75491d04 07/2006 87
What to do if... pHotoFlex / pHotoFlex Turb
6.2 Photometry
88 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb What to do if...
ba75491d04 07/2006 89
What to do if... pHotoFlex / pHotoFlex Turb
6.4 Turbidity
90 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Technical data
7 Technical data
7.1 General data
Storage - 25 °C ... + 65 °C
Ambient conditions Operation 0 °C ... + 50 °C
Climatic class 2
ba75491d04 07/2006 91
Technical data pHotoFlex / pHotoFlex Turb
92 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Technical data
7.1.2 LabStation
7.2 Photometry
ba75491d04 07/2006 93
Technical data pHotoFlex / pHotoFlex Turb
7.4 Turbidity
94 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Accessories, options
8 Accessories, options
8.1 WTW accessories
ba75491d04 07/2006 95
Accessories, options pHotoFlex / pHotoFlex Turb
Thermoprinter You can connect the P3001 to the pHotoFlex (Turb) in the following
ways:
Needle printer You can connect an LQ300 needle printer to the pHotoFlex (Turb) in
one of the following ways:
96 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Accessories, options
Device/cable Model
Barcode reader* ! Handscanner
Datalogic DLC6065-M1
! Handscanner
Datalogic Touch65
Connection cable Datalogic CAB-350
Barcode reader - LabStation
ba75491d04 07/2006 97
Accessories, options pHotoFlex / pHotoFlex Turb
98 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Lists
9 Lists
This chapter provides additional information and orientation aids.
Abbreviations The list of abbreviations explains the indicators and the abbreviations
that appear on the display and in the manual.
Specialist terms The glossary briefly explains the meaning of the specialist terms. How-
ever, terms that should already be familiar to the target group are not
described here.
ba75491d04 07/2006 99
Lists pHotoFlex / pHotoFlex Turb
Abbreviations
Glossary
Adjusting To manipulate a measuring system so that the relevant value (e. g. the
displayed value) differs as little as possible from the correct value or a
value that is regarded as correct, or that the difference remains within
the tolerance.
Analysis specification The exact proceeding to carry out the detection procedure is described
in the analysis specification.
Asymmetry Designation for the offset potential of a pH electrode. It is the measur-
able potential of a symmetrical electrode, the membrane of which is
immersed in a solution with the pH of the nominal electrode zero point
(WTW electrodes: pH = 7).
AutoRange Name of the automatic selection of the measuring range.
AutoRead WTW name for a function to check the stability of the measured value.
Blank value (reagent The evaluation of the photometric measurement always refers to the
blank value) comparison value of a sample without the substance to be determined
(reagent blank value). Thus the influence of the basic absorbance of
the reagents on photometric measurement is compensated for.
Calibration Comparing the value from a measuring system (e. g. the displayed val-
ue) to the correct value or a value that is regarded as correct. Often,
this expression is also used when the measuring system is adjusted at
the same time (see adjusting).
Cell Vessel to take a liquid sample for measurement in a photometer.
The cell material (mostly glass) must have certain optical features to
be suitable for photometry.
Citation forms Different forms of representing a measured concentration value that
can be derived from each other.
The method to determine phosphate, e.g. delivers a measured value
for phosphorous P. This measured value can be alternatively quoted
in the citation forms, PO4, PO4-P or P2O5.
Detection procedure The detection procedure designates the general principle of how a
sample is brought into a form suitable for measurement.
Different methods can be based on the same detection procedure.
Electrode zero point The zero point of a pH electrode is the pH value at which the electro-
motive force of the pH electrode at a specified temperature is zero.
Normally, this is at 25 °C.
Electromotive force of The electromotive force U of the electrode is the measurable electro-
an electrode motive force of an electrode in a solution. It equals the sum of all the
galvanic voltages of the electrode. Its dependency on the pH results in
the electrode function which is characterized by the parameters, slope
and zero point.
Junction The junction is a porous body in the housing wall of reference elec-
trodes or electrolyte bridges. It forms the electrical contact between
two solutions and makes electrolyte exchange more difficult. The ex-
pression, junction, is also used for ground or junction-less transitions.
LED Light Emitting Diode
LEDs are used as the light source in the pHotoFlex (Turb).
Measured parameter The measured parameter is the physical dimension determined by
measuring, e. g. pH, conductivity or DO concentration.
Measured value The measured value is the special value of a measured parameter to
be determined. It is given as a combination of the numerical value and
unit (e. g. 3 m; 0.5 s; 5.2 A; 373.15 K).
Measuring system The measuring system comprises all the devices used for measuring,
e. g. meter and sensor. In addition, there is the cable and possibly an
amplifier, terminal strip and armature.
Method A method comprises a chemical detection procedure and special
method data (calibration line) that is required to evaluate the
measurement results.
How to carry out the method up to measuring with the photometer is
described in the analysis specification.
The pHotoFlex (Turb) contains a database with methods (programs).
Furthermore, user-defined methods can be entered in the database as
well.
Molality Molality is the quantity (in Mol) of a dissolved substance in 1000 g sol-
vent.
MultiCal® WTW name stating that a meter provides several pH calibration pro-
cedures.
Offset potential The measurable potential of a symmetrical electrode, the membrane
of which is immersed in a solution with the pH of the nominal electrode
zero point. The asymmetry is part of the offset potential.
ORP voltage The ORP is caused by oxidizing or reducing substances dissolved in
water if these substances become effective on an electrode surface (e.
g. a gold or platinum surface).
pH value The pH value is a measure of the acidic or basic effect of an aqueous
solution. It corresponds to the negative decadic logarithm of the molal
hydrogen ions activity divided by the unit of the molality. The practical
pH value is the value of a pH measurement.
Potentiometry Name of a measuring technique. The signal (depending on the mea-
sured parameter) of the electrode is the electrical potential. The elec-
trical current remains constant.
Program In the pHotoFlex (Turb), methods with the relevant method data are
stored as programs.
Programs are called up via the assigned program number.
Reset Restoring the original condition of all settings of a measuring system.
Resolution Smallest difference between two measured values that can be dis-
played by a meter.
Slope The slope of a linear calibration function.
Standard solution The standard solution is a solution where the measured value is
known by definition. It is used to calibrate a measuring system.
Temperature function Name of a mathematical function expressing the temperature behav-
ior of a test sample, a sensor or part of a sensor.
Test sample Designation of the test sample ready to be measured. Normally, a test
sample is made by processing the original sample. The test sample
and original sample are identical if the test sample was not processed.
Test set (test) A test set contains all reagents that are required for the photometric
determination of the sample according to the analysis specification.
Zero adjustment Adjusting a photometer with a water-filled cell.
The zero adjustment applies to measuring all measured parameters
(concentration, absorbance, transmission) of a photometer.
10 Index
A system settings ............................ 76, 77
Accumulator pack Diluted samples
charging time .....................................17 measuring ......................................... 50
Air ..........................................................62 Display ............................................. 10, 38
Aligning and marking a cell .......................62 Display illumination ............................ 10, 24
Analysis specification ...............................40 Drift control
Analysis timer ..........................................49 ORP ................................................. 53
Asymmetry of the pH electrode .................55 pH .................................................... 52
Authorized use .........................................14
Automatic switchoff ............................18, 23 F
AutoRead Filter ...................................................... 70
ORP ..................................................53 Firmware update ................................... 107
pH .....................................................52
I
B Initial commissioning ............................... 21
Barcode reader ........................................19 Initialization ............................................. 76
Blank value .............................................43 Interface ................................................. 38
Blank value measurement ........................44
Buffer sets, pH .........................................55
K
Key functions ............................................ 8
C Keys ........................................................ 8
Calibrating
when? ...............................................65
M
Calibration
pH .....................................................55 Measured value display ........................... 27
turbidity measurement .........................65 Measurement dataset .............................. 68
Calibration order ......................................65 Measuring
Calibration points ORP voltage ...................................... 53
pH .....................................................56 pH .................................................... 52
Calibration points and measuring ranges ...65 Measuring menu
Calibration standards ...............................65 pH/ORP ...................................... 36, 54
Cell Measuring range exceeded ...................... 64
insert .................................................24 Measuring the absorbance/transmission ... 45
Cleaning .................................................85 Memory .................................................. 36
Concentration measurement .....................42 Menus (navigation) .................................. 28
Connecting a PC ......................................72 Messages ............................................... 28
Connecting a printer .................................72 Method ................................................... 40
Connecting a sensor ................................10
N
D Navigation .............................................. 27
Data filter ................................................70
Dataset ...................................................68 O
Date and time ..........................................31 Obligations of the operator ....................... 15
Date/time ................................................39 Operating modes .................................... 27
Delivery condition Operating safety ...................................... 14
measuring parameters ........................77
P U
pH calibration evaluation .......................... 57 User-defined programs ............................ 48
Photometric determinations ...................... 40
Power pack ............................................. 17 Z
Print ....................................................... 75
Zero adjustment ...................................... 46
Program ................................................. 40
Programs ............................................... 48
R
Reset ..................................................... 76
RS232 socket assignment ....................... 73
S
Safe operation ........................................ 15
Safety .................................................... 13
Scope of delivery .................................... 17
Single-point calibration
pH .................................................... 59
Slope
pH .................................................... 55
Socket field ............................................. 10
Storage of measured values
storage locations ............................... 68
Storing ................................................... 68
Switching on ........................................... 23
System menu
General ............................................. 38
general information ............................ 41
System settings ...................................... 35
general ................................. 35, 38, 39
T
Target group ........................................... 13
Temperature measurement
pH .................................................... 51
Test set .................................................. 40
Three-point calibration
pH .................................................... 61
Timer ..................................................... 49
Transmitting data .................................... 72
Transmitting measured values .................. 72
Turbidity ................................................. 62
measuring ......................................... 63
Two-point calibration
pH .................................................... 60
A free serial interface (COM port) on your PC and the AK 540/B inter-
face cable is required for this.
Program installation Install the firmware update program on your PC with the
"pHotoFlex_Vx_yy_English.exe" installation program.
Program start Start the "Update_pHotoFlex" program from the Windows start menu.
The program automatically selects the first free serial interface (COM
port). The selected interface is displayed on the left side of the status
line on the screen bottom.
Via the language menu you can change the adjusted language.
After switching the meter off and on again you can check on the start
display whether the meter has taken over the new software version.
Dr.-Karl-Slevogt-Straße 1
D-82362 Weilheim
Germany