Potato Dextrose Agar HIMEDIA PDF

Potato Dextrose Agar M096
Intended use
Potato Dextrose Agar is recommended for the isolation and enumeration of yeasts and moulds from water, dairy, other food
products and clinical samples.
Composition**
Ingredients Gms / Litre
Potatoes, infusion from 2200.000
Dextrose 20.000
Agar 15.000
Final pH ( at 25°C) 5.6±0.2
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 39.09 grams in 1000 ml purified/ distilled water. Heat to boiling to dissolve the medium completely. Sterilize by
autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well before dispensing. In specific work, when pH 3.5 is
required, acidify the medium with sterile 10% tartaric acid. The amount of acid required for 100 ml of sterile, cooled
medium is approximately 1 ml. DO NOT HEAT the medium after addition of the acid.
Principle And Interpretation

Potato Dextrose Agar is recommended by APHA (9) and F.D.A. (4) for plate counts of yeasts and moulds in the examination
of foods and dairy products (3). Potato Dextrose Agar is also used for stimulating sporulation, for maintaining stock cultures
of certain dermatophytes and for differentiation of typical varieties of dermatophytes on the basis of pigment production (8).
It is also recommended by USP (10), BP (2) ,EP (3) and JP (6) for growth of fungi.
Potato infusion and dextrose promote luxuriant fungal growth. Adjusting the pH of the medium by tartaric acid to 3.5,
inhibits the bacterial growth. Heating the medium after acidification should be avoided as it may hydrolyse the agar which
can render the agar unable to solidify.
Type of specimen
Food and dairy samples ; Water samples, Clinical samples- skin scrapings
Specimen Collection and Handling:

For clinical samples follow appropriate techniques for handling specimens as per established guidelines (5,7).
For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (4,9,11).
For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards.(1)
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions

In Vitro diagnostic Use. Read the label before opening the container. Wear protective gloves/protective clothing/eye
protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard
precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be
referred in individual safety data sheets
Performance and Evaluation

Performance of the medium is expected when used as per the direction on the label within the expiry period when stored
at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Please refer disclaimer Overleaf.

HiMedia Laboratories
Technical Data
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Light amber coloured clear to slightly opalescent gel forms in Petri plates.
Reaction
Reaction of 4.1% w/v aqueous solution at 25°C. pH : 5.6±0.2
pH
5.40-5.80
Cultural Response
Cultural characteristics observed after an incubation at 22 - 25°C for 4 - 5 days .
Organism Growth Ascospore
formation
Aspergillus brasiliensis luxuriant Negative
ATCC 16404 (00053*)
Candida albicans ATCC luxuriant Negative
10231 (00054*)
Saccharomyces cerevisiae luxuriant Positive
ATCC 9763 (00058*)
Key : * - Corresponding WDCM numbers
Storage and Shelf Life

Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on
the label. On opening, product should be properly stored dry, after tightly capping the bottle inorder to prevent lump
formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation.
Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly
after use. Use before expiry date on the label.
Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow
established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical
sample must be decontaminated and disposed of in accordance with current laboratory techniques (5,7).
Reference
1. Baird R.B., Eaton A.D., and Rice E.W., (Eds.), 2015, Standard Methods for the Examination of Water and
Wastewater, 23rd ed., APHA, Washington, D.C.
2.British Pharmacopoeia, 2017, The Stationery office British Pharmacopoeia
3.European Pharmacopoeia, 2017, European Dept. for the quality of Medicines.
4.FDA Bacteriological Analytical Manual, 2005, 18th Ed., AOAC, Washington, DC.
5. Isenberg, H.D. Clinical Microbiology Procedures Handbook. 2nd Edition.
6. Japanese Pharmacopoeia, 2016.
7. Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015)
Manual of Clinical Microbiology, 11th Edition. Vol. 1.
8.MacFaddin J. F., 1985, Media for the Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol.1, Williams
and Wilkins, Baltimore
9. Salfinger Y., and Tortorello M.L., 2015, Compendium of Methods for the Microbiological Examination of Foods,
5th Ed., American Public Health Association, Washington, D.C.
10. The United States Pharmacopoeia, 2018, The United States Pharmacopoeial Convention. Rockville, MD.
11.Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th
Ed., APHA Inc., Washington, D.C.
Revision : 003/ 2019
Please refer disclaimer Overleaf.

HiMedia Laboratories
Technical Data
In vitro diagnostic medical

IVD device
CE Marking
30°C Storage temperature
10°C
Do not use if package is

damaged
HiMedia Laboratories Pvt. Limited,

23 Vadhani Industrial Estate,
LBS Marg,Mumbai-86,MS,India
CE Partner 4U ,Esdoornlaan 13, 3951

EC REP
DB Maarn The Netherlands,
www.cepartner 4u.eu
Disclaimer :
User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained in
this and other related HiMedia™ publications. The information contained in this publication is based on our research and development
work and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes to
specifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use but
for laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not
be considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any patents.
HiMedia Laboratories Pvt. Ltd. Reg.office : 23, Vadhani Ind.Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6116 9797 Corporate
office : A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email:
[email protected] Website: www.himedialabs.com

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Potato Dextrose Agar M096

Principle And Interpretation

Specimen Collection and Handling:

Warning and Precautions

Performance and Evaluation

Please refer disclaimer Overleaf.

Key : * - Corresponding WDCM numbers

Storage and Shelf Life

Revision : 003/ 2019

Please refer disclaimer Overleaf.

In vitro diagnostic medical

30°C Storage temperature

Do not use if package is

HiMedia Laboratories Pvt. Limited,

CE Partner 4U ,Esdoornlaan 13, 3951

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