Title: Coronavirus Disease 2019 (COVID-19) During Pregnancy: A Case Series

Preprints (www.preprints.
org) | NOT PEER-REVIEWED | Posted: 25 February 2020
Title: Coronavirus disease 2019 (COVID-19) during pregnancy: a case series
Weiyong LIU PhD1*; Qianli WANG PhD2*; Qi ZHANG PhD3*; Ling CHEN MD1*; Junbo
CHEN PhD2; Bo ZHANG MS1; Yanjun LU PhD1; Shaoshuai WANG MD1; Liming XIA
MD1; Lu HUANG MD1; Kai WANG BS2; Lu LIANG BS4; Yongli ZHANG MS5; Lance
TURTLE PhD6,7; David LISSAUER PhD8,9; Ke LAN PhD3†; Ling FENG MD1†; Hongjie
YU MD,PhD2†; Yingle LIU PhD3†; Ziyong SUN PhD1†
Affiliations:
1. Tongji Hospital, Tongji Medical College, Huazhong University of Science and
Technology, Wuhan, Hubei, China
2. School of Public Health, Fudan University, Key Laboratory of Public Health
Safety, Ministry of Education, Shanghai, China
3. State Key Laboratory of Virology, Modern Virology Research Center, College of
Life Sciences, Wuhan University, Wuhan, Hubei, China
4. West China School of Public Health and West China Fourth Hospital, Sichuan
University, Chengdu, Sichuan, China
5. Savaid Medical School, University of Chinese Academy of Sciences, Beijing,
China
6. National Institute for Health Research Health Protection Research Unit in
Emerging and Zoonotic Infections, Institute of Infection and Global Health,
University of Liverpool, Liverpool, United Kingdom
© 2020 by the author(s). Distributed under a Creative Commons CC BY license.

Preprints (www.preprints.org) | NOT PEER-REVIEWED | Posted: 25 February 2020
7. Liverpool University Hospitals Foundation Trust, member of Liverpool Health
Partners, Liverpool, United Kingdom
8. Women’s and Children’s Health, Institute of life course and medical sciences,
University of Liverpool, United Kingdom
9. The Malawi Liverpool Wellcome Trust Clinical Research Programme, Blantyre,
Malawi
*These authors contributed equally to this work.
† Corresponding author.
Address for Correspondence
Ke Lan, State Key Laboratory of Virology, Modern Virology Research Center,
College of Life Sciences, Wuhan University, Wuhan, 430072, Hubei, China; phone
number: 8627-68754592; email: [email protected]
Ling Feng, Tongji Hospital, Tongji Medical College, Huazhong University of
Science and Technology, Wuhan, 430030, Hubei, China; phone number: 8627-
83663639; email: [email protected]
Hongjie Yu, School of Public Health, Fudan University, Key Laboratory of Public
Health Safety, Ministry of Education, 200032, China; phone number: 8621-
Yingle Liu, State Key Laboratory of Virology, Modern Virology Research Center,
College of Life Sciences, Wuhan University, Wuhan, 430072, Hubei, China; phone
number: 8627-68752938; email: [email protected]

Ziyong Sun, Tongji Hospital, Tongji Medical College, Huazhong University of
Science and Technology, Wuhan, 430030, Hubei, China; phone number: 8627-
Potential conflicts of interest Dr. Hongjie Yu has received research funding
from Sanofi Pasteur, GlaxoSmithKline, Yichang HEC Changjiang Pharmaceutical
Company, and Shanghai Roche Pharmaceuticals Company. None of that research
funding is related to COVID-19. All other authors report no potential conflicts.
Financial support The financial support from the National Mega Project on
Major Infectious Disease Prevention (No. 2017ZX10103005), National Key
Research and Development Program of China (No. 2018YFE0204500), National
Science Fund for Distinguished Young Scholars (No. 81525023), National Science
and Technology Major Project of China (No. 2017ZX10103009-005,
2018ZX10713001-007, 2018ZX10201001-010), and the epidemiological study
on COVID-19 in Shanghai (No. 20411950100). Dr. Turtle is supported by the
Wellcome Trust (grant number 205228/Z/16/Z); the NIHR Health Protection
Research Unit in Emerging and Zoonotic Infections (HPRU-EZI) at University of
Liverpool in partnership with Public Health England (PHE), in collaboration with
Liverpool School of Tropical Medicine. The views expressed are those of the
author(s) and not necessarily those of the NHS, the NIHR, the Department of
Health, or PHE.
Word count (abstract): 253
Word count (main text): 2572

Condensation From this most comprehensive virological assessment to date,
there was no evidence to support vertical transmission of SARS-CoV-2.
Short Title COVID-19 during pregnancy
AJOG at a Glance
A. Why was this study conducted?
The reports regarding the clinical characteristics of COVID-19 in pregnancy are
limited. Only two studies reporting 18 mothers and 19 infants have been published.
Similar to SARS-CoV, vertical transmission of SARS-CoV-2 has not yet been
observed.
B. Key findings
We have performed the most comprehensive virological assessment to date. We
also find no evidence to support vertical transmission of SARS-CoV-2.
C. What does this add to what is known?
It is possible to provide care for SARS-CoV-2 infected mothers during late
pregnancy and both caesarean and vaginal birth without adverse outcomes for
the mother, infant and healthcare staff. Our findings reinforce the need for the
prospective study of overall impact and long-term prognosis of SARS-CoV-2 on
pregnancy and neonate outcomes.

Abstract
Background: Coronavirus disease 2019 (COVID-19) is a new viral respiratory
disease and whether pregnant women are at increased risk of infection is
unknown. Viral pneumonia is an important indirect cause of maternal death.
Little is known about the effects of severe acute respiratory syndrome
coronavirus 2 (SARS-CoV-2) during pregnancy.
Objective: To describe the clinical characteristics of COVID-19 in pregnancy and
their newborn infant, and we sought to explored whether the SARS-CoV-2 can be
intrauterine vertically transmitted.
Study Design: The study was a case series study conducted in the obstetric ward
of Tongji Hospital affiliated to Huazhong University of science and technology,
Wuhan, China. Demographic, clinical, laboratory and radiological profiles of the
SARS-CoV-2 infection case series. A systematic testing procedure for SARS-CoV-2
infection using oropharyngeal swab, placenta tissue, vaginal mucus, and breast
milk of mothers. and oropharyngeal swab, umbilical cord blood, and serum of
newborns was conducted.
Results: We have conducted the most thorough virological assessment to date,
and we include a longer clinical observation in mother-infant dyads during
hospitalization. The clinical course and outcomes of three pregnant women who
acquired SARS-CoV-2 infection late pregnancy are described in mother-infant
dyads. Two had caesarean delivery in their third trimester. All patients showed
an uneventful perinatal course, and a successful outcome. No infants became

infected by vertical transmission or during delivery.
Conclusion: No evidence to suggest the potential risk of intrauterine vertical
transmission in the case series and further in-depth study is needed. Both the
pregnancy woman and infant showed fewer adverse maternal and neonatal
outcomes.
Key words pregnancy, neonate, COVID-19, SARS-CoV-2, vertical transmission

Introduction
An outbreak of respiratory illness, named coronavirus disease 2019 (COVID-
19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
emerged in Wuhan, China in late 2019, resulting in tens of thousands of
confirmed cases in China, with additional cases identified in a growing number of
other countries1-3.
The whole population is thought susceptible to SARS-CoV-2 and it is
suspected that pregnant women are likely to be at increased risk of severe
infections, with the potential for adverse maternal and perinatal outcomes.4 This
concern is based on experience of the severe acute respiratory syndrome (SARS)
and Middle East respiratory syndrome (MERS), caused by related coronaviruses,
which were found to be associated with worse outcome during pregnancy. 5-8
There are no previous reports suggesting mother to child transmission of
infections caused by the coronavirus family, but at present this is still unknown
for SARS-CoV-2. The reports regarding the clinical characteristics of COVID-19 in
pregnancy are limited. Two recent publications by Chen et al. and Zhu et al.
describing COVID-19 in pregnancy observed few adverse outcomes, with no
evidence for vertical transmission currently.9 10 Herein, we report three pregnant
patients who acquired the infection during the last trimester in Wuhan, with a
longer period of clinical observation and outcomes described in the mothers and
their newborn infants.

Materials and Methods
Study Design and Sources of Cases
Between February 2, 2020 and February 5, 2020, three women in late
pregnancy diagnosed with COVID-19 according to New Coronavirus Pneumonia
Prevention and Control Program (Trail 5th edition) published by the National
Health Commission of China11 enrolled in the obstetric ward of Tongji Hospital, a
tertiary hospital in Wuhan. No cases were missed during this period. Informed
consent was obtained from each pregnant woman. For the newborn infant, proxy
oral informed consent was provided by a parent or legal guardian. The case
series study was approved by the institutional ethics board of Tongji Hospital
affiliated to Huazhong University of science and technology (Approval Number
TJ-C2030).
Procedures and data collection
We reviewed the perinatal demographic, clinical, laboratory and radiological
data from the participants electronic medical records. Data available included
detailed information of clinical presentation, obstetric management and
complications, investigations, treatments and maternal and neonatal outcomes.
Additional epidemiological data were obtained through telephone interviews
with participants and their families. If data were missing or unclear from the
medical records, we obtained data by direct communication with attending
doctors. The clinical outcomes were followed up to Feb 13, 2020. The data were
reviewed by a trained team of obstetricians and clinical laboratory doctors. The

disease onset date was defined as the day when the symptom related to COVID-
19 was noticed.
Diagnostic testing for SARS-CoV-2
Oropharyngeal swab specimens were collected from mothers and their
neonates. After collection, swabs were placed in viral transport medium.
Specimens were stored between 2°C and 8°C until testing. Total RNA was
extracted using automated Nucleic Acid Extraction System 9600E (Xi'an
TianLong Science and Technology Co., Ltd., Xi’an, China). Real-time reverse
transcription polymerase chain reaction (RT-PCR) assay for SARS-CoV-2 was
performed using a SARS-CoV-2 ORF1ab/N gene detection kit (Shanghai Huirui
Biotechnology Co.,Ltd, Shanghai, China), a product based on the recommendation
of the National Institute for Viral Disease Control and Prevention, Chinese Center
for Disease Control and Prevention
(http://ivdc.chinacdc.cn/kyjz/202001/t20200121_211337.html). Open reading
frame 1ab gene (ORF1ab) and nucleocapsid gene (N) were amplified and tested
using the following primers and probes: ORF1ab forward primer 5'-
CCCTGTGGGTTTTACACTTAA-3', ORF1ab reverse primer 5'-
ACGATTGTGCATCAGCTGA-3', ORF1ab probe 5'-FAM-
CCGTCTGCGGTATGTGGAAAGGTTATGG-BHQ1-3', N forward primer 5'-
GGGGAACTTCTCCTGCTAGAAT-3', N reverse primer 5'-
CAGACATTTTGCTCTCAAGCTG-3', N probe 5'-ROX-TTGCTGCTGCTTGACAGATT-
TAMRA-3'. Five μL of RNA solution was added into the 20 μL reaction mixture.
10
Real-time RT-PCR was performed using the following cycling parameters: reverse
transcription at 50 °C for 15 minutes, denaturation at 95 °C for 5 minutes, 45
cycles of denaturation at 94 °C for 10 seconds and extension at 55 °C for 45
seconds, fluorescence was measured in each cycle. A negative real-time RT-PCR
result was defined as undetectable or a cycle threshold value (Ct-value) of >=
39.2, a positive result was defined as a Ct-value <35. Results were reported
positive when both ORF1ab gene and N gene tested to be positive. Specimens
tested as Ct-value of >=35 and <39.2 were retested for confirmation, a retest Ct-
value of <39.2 was treated as positive, otherwise negative.
Results
Between the 2nd and 5th February 2020, the obstetrics ward admitted a
total of 17 pregnant women, of which three (17.6%) were diagnosed with COVID-
19. All the three patients were from Wuhan city. The patient’s ages ranged from
30 to 34 years. All three pregnancies were infected in the third trimester and
delivered at term. The newborn infants survived and were uninfected. The three
patients had previously been cared for in different obstetric wards during the
same period. All the Healthcare Personnel (HCP) in the obstetric and newborn
infant wards used appropriate personal protective equipment (PPE) according to
current guidance12.
Patient 1 and Newborn infant 1
Patient 1 was a 34-year-old woman (gravida 2, para 0) with a history of

11
hypothyroidism for 4 years and epiglottic cysts. The potential exposure to SARS-
CoV-2 reported was a visit to Wuhan Children’s hospital for a routine
examination on Jan. 27, 2020. She reported the initial onset of fever (37.8℃) on
1st February 2020 (40 weeks gestational age), eight hours before presentation to
the obstetric ward in Tongji hospital on 2nd February 2020.
A systematic testing procedure for SARS-CoV-2 infection was used, including
computed tomography (CT) of the chest and serial RT-PCR assays. On admission
chest auscultation was normal, however chest CT revealed bilateral infiltrates,
worse on the left, including involvement of the upper lobe of left lung, localized
emphysema in the right lower lobe and a few linear fibrotic changes in the
middle lobe of the right lung (figure 1). Repeat CT scans of the chest, performed
on 8th February (eight days after onset) and 13th February (13 days after onset),
showed progression of the changes compared with the previous study (Figure 1).
A positive result for SARS-CoV-2 PCR performed on an oropharyngeal swab was
obtained on 2nd February. Subsequent RT-PCR assays on oropharyngeal swab
specimens were positive on 5th February, and turned negative on the 7th and 9th
February. SARS-CoV-2 RT-PCR tests were performed on breast milk, placenta, and
vaginal mucus from the 2nd February 2 to 12th February and all results were
negative. A positive result for SARS-CoV-2 PCR performed on faeces was obtained
on 7th February and was negative on 12th February (on an anal swab). A
respiratory sputum specimen was obtained from her husband tested negative for
SARS-CoV-2 by RT-PCR on 2nd February.

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A 3,250 g male newborn was delivered by cesarean delivery on 2nd February
(gestational age 40 weeks). There was evidence of meconium staining of the
embryonic membranes. Apgar scores at 1 and 5 minutes were 8 and 9,
respectively. After delivery, the infant was immediately moved to a sealed
incubator with a separate air exchange system in the neonatal ward and received
initial care from HCP completely protected according to the current PPE
guidelines12. Efforts were made during delivery to minimize and exposure to
maternal blood and neonate washing was conducted in neonate ward. The infant
was not breast fed. The neonate presented chronic fetal distress in utero,
chorioamnionitis and Meconium Stained Amniotic Fluid (MSAF). All of the
following samples of newborn tested negative for SARS-CoV-2 PCR: umbilical
cord blood, plasma serum and whole blood collected at 34min after birth,
oropharyngeal swab collected on one day after delivery. The clinical details of
mother and infant 1 are summarised in Table 1 and Figure 2.
The patient was transferred back to the maternity ward after delivery.
Oxygen therapy, antiviral, antibiotics and anti-inflammatory treatment were
administered. The antiviral therapy including: atomized inhalation of interferon
(40μg, bid) and ganciclovir (0.25g, IV). She did not require ventilatory support.
Patient 2 was a 34-year-old woman (gravida 3, para 1) with no history of
underlying medical conditions. The potential exposure to SARS-CoV-2 reported
was a stay in BAIJIA maternity hospital in Wuhan for a routine obstetric

13
examination from 25th to 26th January 2020. No other exposure to SARS-CoV-2
was identified and she had consistently worn a mask for protection. Her family
members reported no respiratory symptoms. She reported the initial onset of
fever on 30th January 2020 (37 weeks gestational age). She visited the outpatient
department of Tongji Hospital on 30th January and was admitted to the obstetric
ward on 5th February 2020.
A systematic assessment for SARS-CoV-2 infection was conducted similar to
patient 1. CT of the chest was performed on 30th January and showed bilateral
ground glass opacity (GGO), consolidation, nodules in the left lower lobe and
patchy consolidation in the right middle lobe. A repeat chest CT scan, performed
on 13th February (15 days after onset), showed progression of the changes
compared with the previous study (Figure 1). An oropharyngeal specimen
collected on admission (5th February 2020, seven days after illness onset, one day
before delivery) tested positive for SARS-CoV-2 by RT-PCR with a CT value of 28,
and was negative on 13th February.
The patient returned to the maternity ward after delivery. Oxygen therapy,
antibiotic and anti-inflammatory treatment were used. Oral Arbidol
hydrochloride (a broad-spectrum antiviral compound that blocks viral fusion)
was administered (3g, qd). She did not require ventilator support.
A 3,250g female newborn was delivered by cesarean delivery on 6th February
(gestational age 38+4 weeks). Apgar scores at 1 and 5 minutes were 8 and 9,
respectively. After delivery, the biosafety protection and medical care was the
14
same as Infant 1 and neonate washing was conducted in neonatal ward. The
newborn showed slightly decreased responsiveness and muscle tone, which
recovered by 7th February. SARS-CoV-2 RT-PCR tests were performed on
oropharyngeal swabs, serum, whole blood, urine and faeces collected 27 minutes
after birth and all results were negative. Subsequent RT-PCR assays on
oropharyngeal swab, urine and faeces were again negative from 7th through 13th
February. The clinical details of mother and infant 2 are shown in Table 1 and
Figure 2.
Patient 3 was a 30-year-old woman (gravida 3, para 1) with a history of
gestational hypertension during her first pregnancy. The potential exposure to
SARS-CoV-2 was she reported a visit to Tongji hospital for a routine obstetric
examination on 20th Jauary 2020. No other exposure to SARS-CoV-2 was
identified and she consistently wore a mask for protection. Her family members
reported no respiratory symptoms. She reported the initial onset of cough on
25th January 2020 (37 weeks gestational age). She was admitted to the obstetric
ward on 5th February 2020.
The assessment for SARS-CoV-2 infection (similar to patient 1 and 2) showed
GGO, subsolid patchy and linear fibrosis in the left lung, increased mediastinal
small lymph nodes on a chest CT scan performed on admission. A further chest
CT scan was performed on 10th February (16 days after onset) showed
improvement compared with the previous imaging (Figure 1). The SARS-CoV-2
15
RT-PCR performed on an oropharyngeal swab specimen gave a positive result on
6th February. Follow up oropharyngeal swab specimens were collected and tested
positive on 10th February but turned negative on 11th February. SARS-CoV-2 RT-
PCR was performed on breast milk, anal swab, and vaginal mucus on 11th
February, and all results were negative.
A 3,670g female newborn was delivered by vaginal delivery based on
maternal request on 9th February. Apgar scores at 1 and 5 minutes were 8 and 9,
respectively. After delivery, the biosafety protection and medical care was the
same as Infant 1 and 2, and neonate was washed in the neonatal ward. SARS-CoV-
2 RT-PCR tests were performed on oropharyngeal swabs, plasma, whole blood,
urine and faeces from 10th and 11th February and all results were negative.
Detailed information of mother and infant 3 is shown in Table 1 and Figure 2.
The patient was transferred back to the maternity ward after delivery.
Oxygen and antiviral therapy (Arbidol hydrochloride 3g, qid orally) were
administered. She did not require ventilator support.
Comment
Principal findings
We report three patients with confirmed SARS-CoV-2 infection during the
3rd trimester of pregnancy living in Wuhan. The patients showed an uneventful
perinatal course, and successful outcomes. No infants became infected by vertical
transmission or during delivery. Serial specimens collected from the newborn

16
infants at different time points after birth were all negative for SARS-CoV-2 by
RT-PCR.
Our patients initially presented with mild symptoms, only fever or cough,
and all showed chest CT abnormalities during infection. There was progression
of CT abnormalities in two patients (patient 1 and 2), and improvement in the CT
appearance of patient 3 on the 16th day of illness.
Results in context
There are limited data concerning the clinical features and impact of SARS-
CoV-2 infection during pregnancy and the perinatal outcome of patients
diagnosed with COVID-19.
One of the infants in our study showed Meconium Stained Amniotic Fluid
(MSAF), but all outcomes were good. However, Zhu and colleagues10, reported 6
out of 10 neonates born pre-term, and Chen et al9, who reported 6 out of 9
neonates born preterm. In the study of Zhu et al a number of whom experienced
significant complications, and one died. Therefore evidence is building that
COVID-19 pregnancy is not without risk, albeit less serious than SARS, in which
adverse outcomes were reported for 10 of 12 pregnancies, including 4 first
trimester miscarriages.5 To date, all affected mothers with COVID-19 have
recovered.9 10
Research implications
The overall impact and long-term prognosis of SARS-CoV-2 on pregnancy and
neonatal outcomes needs further evaluation. The possibility of intrauterine

17
vertical transmission of SARS-CoV-2 requires in-depth investigation including
placental histopathology and PCR to look for SARS-CoV-2. Moreover, a
prospective study of COVID-19 in pregnant women in from early pregnancy, to
evaluate the potential for vertical transmission is urgently needed.
Strengths and limitations of the study
In this case series, which we believe has performed the most comprehensive
testing for SARS-CoV-2 in pregnancy and in newborns, we find no evidence to
support the vertical transmission of SARS-CoV-2. The conclusions of this study
are consistent with the finding the two previous studies on COVID-19 infection in
pregnancy women 9 10 and with observations in SARS. Two neonates with COVID-
19 have been reported, but in each case infection after birth cannot be
excluded.13
Importantly, we and others have now shown that it is possible to provide
care for SARS-CoV-2 infected mothers during late pregnancy and both caesarean
and vaginal birth without adverse outcomes for the mother, infant and healthcare
staff. This information will benefit those planning care for non-infection related
medical interventions in patients who may be infected with SARS-CoV-2. It
should be noted, however, that we only report three mother-infant dyads, all of
whom were not severely affected and did not have any evidence of virus
detectable in blood.
This study has several limitations. First, due to the limited sample size of
study subjects, only a limited number of intrauterine specimens were obtained

18
during labor. Second, we retrospectively evaluated the clinical data collected
from both patients and newborns.
Conclusion
Despite the lack of evidence of intrauterine vertical transmission, infections
that can occur during vaginal delivery are worthy of concern. Therefore,
clinicians should remain vigilant for potential vertical transmission events of
SARS-CoV-2 during late pregnancy or delivery. The approach of medical
protection of newborns after birth is successful and needs further strengthening,
including use of personal protection precautions to reduce the risk of
transmission.
Acknowledgment We are grateful to Taikang Insurance Group Co., Ltd and
Beijing Taikang Yicai Foundation for their great support to this work. We thank
all the participating women and their children.
Contributors Dr. Ziyong Zhang, Dr. Yingle Liu, Dr. Hongjie Yu, Dr. Ling Feng and
Dr. Ke Lan conceptualised the study design. Dr. Yingle Liu, Weiyong Liu, Dr.
Shaoshuai Wang, Dr. LimingXia, Dr. Lu Huang, Dr. Junbo Chen, Mr. Kai Wang, Ms.
Liang Lu and Ms. Yongli Zhang acquired the data and interpreted the data; Dr.
Weiyong Liu, Dr. Qianli Wang. Dr. Qi Zhang and Dr. Ling Chen drafted the
manuscript; Dr. Lance Turtle and Dr. David Lissauer contributed to revising the
manuscript; Dr. Ling Chen and Dr. Shaoshuai Wang collected specimens; Dr.
19
Weiyong Liu, Mr. Bo Zhang and Dr. Yanjun Lu did the laboratory tests.
20
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Table 1 Demographics, Clinical characteristics, Treatment and Laboratory Findings of Three Pregnant Women and Newborn Infant
Subject No. 1 2 3
Pregnant Women
Demographics, obstetric characteristics, and clinical outcomes
Age (Years) 34 34 30
Occupation Employee Self-employed Self-employed
Hypothyroidism for 4 years / Gestational
Underlying comorbidities None
Epiglottic cysts diabetes
Other medical histories during pregnancy None Scarred uterus / Accreta placenta None
Gravidity 2 3 3
Parity 0 1 1
History of abortion Yes Yes Yes
Start of pregnancy Apr.29, 2019 May.11, 2019 May.7, 2019
Date of delivery Feb. 2, 2020 Feb. 6, 2020 Feb. 5, 2020
End of pregnancy, gestational age, Week/trimester 40/third 38+4/third 39+5/third
Vaginal delivery at
Delivery details Cesarean delivery at term Cesarean delivery at term
term
Indication of cesarean delivery COVID-19 COVID-19
Intrauterine fetal distress Yes No No
Embryonic membrane Thickening / Stained yellow Normal Normal
Remained in
Maternal outcome Remained in hospital Remained in hospital
hospital
Survived / Chronic fetal distress in
Fetal outcome Survived Survived
utero/ Chorioamnionitis
Clinical characteristics and treatment
23
Signs and symptoms at admission

Fever Yes Yes No
Cough No Yes Yes
Temperature (℃) 37.8 38.0 37.0
Onset to hospitalization (Day) 1 6 10
Gestational age at illness onset (Week) 40 37 37
Chest CT findings
CT evidence of pneumonia Yes Yes Yes
Unilateral pneumonia No No Yes
Bilateral pneumonia Yes Yes No
Treatment
Oxygen therapy Yes Yes Yes
Antiviral treatment Yes Yes Yes
Ganciclovir Yes No No
Abedore hydrochloride No Yes Yes
Interferon Yes No No
Antibiotic treatment Yes Yes No
Glucocorticoids Yes Yes No
Laboratory results
Blood routine
Leucocytes (× 10⁹ per L; normal range 3.5–9.5) 11.96 10.17 7.63
Neutrophils (× 10⁹ per L; normal range 1.8–6.3) 9.97 8.25 5.54
Lymphocytes (× 10⁹ per L; normal range 1.1–3.2) 11.50 1.41 1.7
Eosinophils (× 10⁹ per L; normal range 0.02–0.5) 0.00 0.01 0.04
Basophil (× 10⁹ per L; normal range 0.00–0.1) 0.01 0.01 0.1
Platelets (× 10⁹ per L; normal range 125.0–350.0) 81.00 152
Haemoglobin (g/L; normal range 115.0–150.0) 129.00 145 114
Blood biochemistry
24
Albumin (g/L; normal range 40.0–55.0) 32.2 36 32.5

Alanine aminotransferase (U/L; normal range 0.0–33.0) 6.5 14 9
Aspartate aminotransferase (U/L; normal range 0.0–32.0) 25.7 22.1 14
Infection-related biomarkers
Procalcitonin (ng/mL; normal range 0.02–0.05) 0.03 0.07 NA
C-reactive protein (mg/L; normal range 0.0–1.0) NA 11.5 14.7
Interleukin-6 (pg/mL; normal range 0.0–7.0) 44.18 43.72 NA
Erythrocyte sedimentation rate (mm/h; normal range 0.0–20.0) 26 NA NA
Serum ferritin (ng/mL; normal range 15.0–150.0) 151.9 NA 21.1
Newborn Infant
Demographics
Gender Male Female Male
Gestational age (Weeks) 40 38+4 39+5
No. of embryo Singleton Singleton Singleton
Birthweight (g) 3250 3250 3670
1 min Apagr score 8 8 8
5 min Apagr score 9 9 9
Meconium Stained Amniotic Fluid Slight decreased responsiveness and
Clinical characteristics None
(MSAF) muscle tension
Laboratory results
Leucocytes (× 10⁹ per L; normal range 5–20) 13.24 22.19 18
Neutrophils (× 10⁹ per L; normal range 2–10) 9.52 16.4 11.7
Lymphocytes (× 10⁹ per L; normal range 3–8) 2.43 3.31 3.8
Eosinophils (× 10⁹ per L; normal range 0.2–2) 0.11 0.35 0.88
Basophil (× 10⁹ per L; normal range 0.0–0.2) 0.01 0.2 0.1
Platelets (× 10⁹ per L; normal range 150.0–300.0) 218 137 208
Haemoglobin (g/L; normal range 148.0–190.0) 160 158 195
25
Blood biochemistry
Albumin (g/L; normal range 38.0–54.0) 38.3 32.6 32.1
Alanine aminotransferase (U/L; normal range 0.0–41.0) 11 18 24
Aspartate aminotransferase (U/L; normal range 0.0–40.0) 143 28 150
Infection-related biomarkers
Procalcitonin (ng/mL; normal range 0.0–5.0) NA 0.23 0.33
C-reactive protein (mg/L; normal range 0.0–5.0) ＜0.5 ＜0.1 NA
Abbreviations: NA, not available; COVID-19, 2019 novel coronavirus disease; Abnormal value are of laboratory results are highlighted in bold.
26
Figure legend
Figure 1. Chest CT images of three patients who diagnosed with SARS-CoV-2
infection.
A: Patient 1 at two days after illness onset (A1) and eight days after onset (A2). CT
images show infiltrates and linear fibrosis. A2 showed progressive abnormality
compared to A1.
B: Patient 2 on the illness onset (B1) and 15 days after onset (B2). CT images shows
GGO, patchy consolidation, pleural effusion. B2 showed progressive abnormality
compared to B1.
C: Patient 3 at 11 days after illness onset (C1) and 16 days after illness onset (C2).
CT images show GGO, subsolid patch and linear fibrosis. C2 showed absorption
and improvement compared to C1.
Figure 2. Timeline of symptoms, clinical events, CT scan and laboratory test
according to day of illness.
(A) Patient 1and her newborn; (B) Patient 2 and her newborn; (C) Patient 3 and
her newborn.
27
Figure 1
28
Figure 2

Title: Coronavirus Disease 2019 (COVID-19) During Pregnancy: A Case Series

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Preprints (www.preprints.

org) | NOT PEER-REVIEWED | Posted: 25 February 2020

Title: Coronavirus disease 2019 (COVID-19) during pregnancy: a case series

YU MD,PhD2†; Yingle LIU PhD3†; Ziyong SUN PhD1†

1. Tongji Hospital, Tongji Medical College, Huazhong University of Science and

Technology, Wuhan, Hubei, China

2. School of Public Health, Fudan University, Key Laboratory of Public Health

Safety, Ministry of Education, Shanghai, China

3. State Key Laboratory of Virology, Modern Virology Research Center, College of

Life Sciences, Wuhan University, Wuhan, Hubei, China

University, Chengdu, Sichuan, China

5. Savaid Medical School, University of Chinese Academy of Sciences, Beijing,

6. National Institute for Health Research Health Protection Research Unit in

Emerging and Zoonotic Infections, Institute of Infection and Global Health,

University of Liverpool, Liverpool, United Kingdom

© 2020 by the author(s). Distributed under a Creative Commons CC BY license.

7. Liverpool University Hospitals Foundation Trust, member of Liverpool Health

Partners, Liverpool, United Kingdom

University of Liverpool, United Kingdom

9. The Malawi Liverpool Wellcome Trust Clinical Research Programme, Blantyre,

*These authors contributed equally to this work.

Address for Correspondence

Ke Lan, State Key Laboratory of Virology, Modern Virology Research Center,

number: 8627-68754592; email: [email protected]

Ling Feng, Tongji Hospital, Tongji Medical College, Huazhong University of

83663639; email: [email protected]

Health Safety, Ministry of Education, 200032, China; phone number: 8621-

54237628; email: [email protected]

number: 8627-68752938; email: [email protected]

Ziyong Sun, Tongji Hospital, Tongji Medical College, Huazhong University of

83663639; email: [email protected]

Potential conflicts of interest Dr. Hongjie Yu has received research funding

from Sanofi Pasteur, GlaxoSmithKline, Yichang HEC Changjiang Pharmaceutical

Company, and Shanghai Roche Pharmaceuticals Company. None of that research

funding is related to COVID-19. All other authors report no potential conflicts.

Major Infectious Disease Prevention (No. 2017ZX10103005), National Key

Research and Development Program of China (No. 2018YFE0204500), National

and Technology Major Project of China (No. 2017ZX10103009-005,

2018ZX10713001-007, 2018ZX10201001-010), and the epidemiological study

on COVID-19 in Shanghai (No. 20411950100). Dr. Turtle is supported by the

Wellcome Trust (grant number 205228/Z/16/Z); the NIHR Health Protection

Research Unit in Emerging and Zoonotic Infections (HPRU-EZI) at University of

Liverpool in partnership with Public Health England (PHE), in collaboration with

Word count (abstract): 253

Word count (main text): 2572

Condensation From this most comprehensive virological assessment to date,

there was no evidence to support vertical transmission of SARS-CoV-2.

Short Title COVID-19 during pregnancy

A. Why was this study conducted?

The reports regarding the clinical characteristics of COVID-19 in pregnancy are

Similar to SARS-CoV, vertical transmission of SARS-CoV-2 has not yet been

We have performed the most comprehensive virological assessment to date. We

also find no evidence to support vertical transmission of SARS-CoV-2.

C. What does this add to what is known?

It is possible to provide care for SARS-CoV-2 infected mothers during late

prospective study of overall impact and long-term prognosis of SARS-CoV-2 on

pregnancy and neonate outcomes.

Background: Coronavirus disease 2019 (COVID-19) is a new viral respiratory