Operator's Manual: Automated

AUTOMATED
HAEMATOLOGY
CELL COUNTER
Operator’s Manual HUMAN: 2019-02
Indication for Use :

The DLB5 is an automatic haematology analyzer used to quantify formed elements (erythrocytes,
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cell count (3 PART : Lymphocytes, Monocytes, Granulocytes / 5 PART : Lymphocytes, Monocytes,
Neutrophiles, Eosinophiles, Basophiles).
DIALAB® Produktion und Vertrieb von chemisch-technischen Produkten und Laborinstrumenten Gesellschaft m.b.H.
DIALAB® GmbH • IZ NOE-Sued •Hondastrasse, Objekt M55 • A-2351 Wiener Neudorf
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www.dialab.at
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Issued on: 2019-0-
Last revision on: 2019-0-
Copyright © of Dialab.
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form or any other form of this manual is strictly forbidden without a preliminary written
agreement of Dialab company.
This machine, included the software and the computing keys, as well as the processes
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royalties.
TABLE OF
CONTENTS
DLB5
3
TABLE OF CONTENTS
TABLE OF CONTENTS
INTRODUCTION ................................................................................ 9
1. WARNINGS AND CAUTIONS .........................................11

1.1 GENERAL INFORMATIONS .............................................. 13
1.2 OPERATORS .......................................................................... 13
1.3 INSTALLATION AND UTILIZATION ................................. 13
1.4 PRECAUTIONS ..................................................................... 14
1.4.1 Against electrical hazard ....................................................... 14
1.4.2 Against Biohazard ................................................................. 15
1.5 REAGENTS HANDLING ...................................................... 15
1.6 MAINTENANCE ..................................................................... 16
1.7 DISPOSAL OF WASTES AND DEVICE .......................... 16
2. INSTALLATION PRESENTATION ................................ 17

2.1 INSTALLATION ...................................................................... 19
2.1.1 Check equipment .................................................................. 19
2.1.2 Instrument preparation .......................................................... 20
2.1.3 Reagent Pack Installation ..................................................... 22
2.1.4 Electrical connections ........................................................... 25
2.2 PRESENTATION .................................................................... 26
2.2.1 Front panel presentation ....................................................... 26
2.2.2 External alphanumerical keyboard use ................................. 27
2.3 SETTING UP ADVICE .......................................................... 28
:RUNHQYLURQPHQW ................................................................. 28
2.3.2 Power connection ................................................................. 28
3. FIRST UTILIZATION ............................................................. 29

3.1 POWER UP ............................................................................. 31
3.1.1 Self-tests ............................................................................... 31
3.1.2 Priming reagents ................................................................... 31
3.2 PRINTOUT ............................................................................... 32
3.2.1 Printers.................................................................................. 32
3.2.2 Printer paper installation ....................................................... 32
3.2.3 Example of ticket................................................................... 33
.../...
DLB5
5
TABLE OF CONTENTS
3.3 RUN AN ANALYSIS ............................................................... 35

*HQHUDODGYLFH ...................................................................... 35
3.3.2 First analysis after installation ............................................... 36
3.3.3 Start screen interpretation..................................................... 37
,GHQWL¿FDWLRQ .......................................................................... 38
3.3.5 Results .................................................................................. 40
3.3.5.1 Normal Range Alarm .......................................... 41
3.3.5.2 Counting Alarms ................................................. 41
8SGDWH,GHQWL¿FDWLRQVFUHHQ ............................... 41
3.3.5.4 Send results to a computer ................................ 41
3.3.5.5 Results printout ....................................................... 42
3.3.5.6 Dilution x 2............................................................... 42
4 CONFIGURATION .................................................................. 43
4.1 MAIN MENU ............................................................................ 45
4.2 RECALL ANALYSIS MENU ................................................ 46
4.3 PARAMETERS MENU ......................................................... 47
4.3.1 Edit name Blood Bank ........................................................... 48
4.3.2 Modify Blood Bank ................................................................. 48
4.3.3 Calibration.............................................................................. 50
4.3.4 User heading ......................................................................... 51
4.3.5 Quality control ........................................................................ 52
4.4 SYSTEM MENU ..................................................................... 53
4.4.1 Time setup ............................................................................. 53
4.4.2 Date setup ............................................................................. 53
4.4.3 Country (Localization ) ........................................................... 54
4.4.4 Communication ...................................................................... 54
4.4.5 Printout .................................................................................. 55
4.4.6 Application sub menu ............................................................ 56
4.4.6.1 Information ......................................................... 56
'ULYHUV ................................................................ 56
4.4.6.3 Hardware ............................................................ 57
4.4.6.4 Units ................................................................... 57
4.4.6.5 Restore banks .................................................... 58
4.5 PRIME/RINSE MENU ........................................................... 59
4.5.1 Priming reagents.................................................................... 59
4.5.2 Priming rinse solution ............................................................ 59
4.5.3 Draining ................................................................................. 59
4.5.4 Hb Reference......................................................................... 59
4.5.5 Unblock aperture ................................................................... 59
4.5.6 H2O Rinsing ........................................................................... 60
4.6 STANDBY MENU ................................................................... 61
4.7 CLEAR ANALYSES MENU ................................................. 63
.../...
DLB5
6
TABLE OF CONTENTS
5 QUALITY CONTROL ........................................................... 65

4.0 USE OF BIOLOGICAL CONTROLS ................................ 67
5.1 RUN ANALYSIS ..................................................................... 69
5.2 RECALL ANALYSIS ............................................................. 70
5.3 REPORTS ................................................................................ 71
5.3.1 Blood analysis report ............................................................. 72
5.3.2 Quality control report ............................................................. 72
5.3.2.1 Q. C. Listing ........................................................ 72
5.3.2.2 L.J. Graph ........................................................... 73
6 MS-CARDS .................................................................................. 75
6.1 REAGENT MS-CARD .......................................................... 77
Blood Banks ..................................................................................... 78
6.2 BLOOD BANK MS-CARD ................................................... 79
6.3 CONTROL BLOOD MS-CARD .......................................... 80
6.4 ADMIN MS-CARD ................................................................. 81
7 CALIBRATION .......................................................................... 83
7.1 BANK CALIBRATION .......................................................... 85
7.2 AUTO CALIBRATION*3 ....................................................... 86
7.3 MANUAL CALIBRATION .................................................... 88
8 MAINTENANCE ....................................................................... 89
8.0 SAFETY ................................................................................... 91
8.1 INSTRUCTIONS FOR USE ................................................. 93
8.2 REAGENT PACK REPLACEMENT .................................. 94
8.3 BLOOD CLOT FILTERS ...................................................... 95
8.4 UPDATING PROGRAM ....................................................... 96
8.5 MEASURING CHAMBER ACCESS ................................. 98
8.6 AIR PUMP ACCESS ............................................................. 98
.../...
DLB5
7
TABLE OF CONTENTS
9 EXPORT DATA ......................................................................... 99

9.1 COMPUTER CONNECTION ........................................... 101
9.1.1 Hardware connection ......................................................... 101
9.1.2 Software connection........................................................... 101
9.1.3 Operating mode ................................................................. 101
9.2 REQUESTS ........................................................................... 102
6RIWZDUH5HYLVLRQQXPEHUUHTXHVW .................................... 102
9.2.2 Set decimal separator for decimal results .......................... 102
6HW&KDUDFWHUVWULQJUHFHLYLQJIRUPDW................................. 102
9.2.4 Stored analyses number request ....................................... 103
/DVWDQDO\VLVVSHFL¿FDWLRQUHTXHVW.................................... 103
6SHFL¿FDWLRQRIDQDQDO\VLVE\LWVVHTXHQFHQXPEHU .......... 104
$OOVWRUHGDQDO\VHVVSHFL¿FDWLRQUHTXHVW ........................... 104
6SHFL¿FDWLRQRIDOOWKHDQDO\VHVIRUDVSHFL¿FGD\ ............. 104
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VSHFL¿FGD\ ........................................................................ 105
+LVWRJUDPVE\DGH¿QHGVHTXHQFHQXPEHU .....................105
+DHPDWRORJ\UHVXOWVE\DGH¿QHGVHTXHQFHQXPEHU ....... 106
9.2.12 Haematology normal range by a blood bank number ....... 109
9.3 AUTO/MANUAL TRANSMISSION .................................. 110
10 APPENDIX ................................................................................. 113

10.1 DLB5 MAIN FEATURES ................................................ 115
10.1.1 Main medical features...................................................... 115
10.1.2 Main software features ....................................................... 115
10.1.3 Mechanical characteristics ................................................. 116
10.1.4 Performance characteristics............................................... 117
10.2 NORMAL RANGE ............................................................. 119
10.3 3 PART-DIFF. CALIBRATION ........................................ 120
10.4 CONNECTORS ................................................................. 122
10.5 PC CONNECTION .............................................................123
10.6 METHOD LIMITATION - INTERFERENCES ...............124
10.7 REAGENTS INSTRUCTION ...........................................126
10.8 ERROR MESSAGES..............................................................130
10.9 LICENSE AGREEMENT ...................................................135
MENUS AND SCREENS ..................................................136
DLB5
8
INTRODUCTION
INTRODUCTION
The DLB5 is an automatic haematology analyzer use to quantify formed elements (erythrocytes,
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cell count (3 PART : Lymphocytes, Monocytes, Granulocytes / 5 PART : Lymphocytes, Monocytes,
Neutrophiles, Eosinophiles, Basophiles).
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Use claimed :
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diagnostic use only. Like any automatic counter-hematology analyzer it
performs only approach to CBC (Complete Blood Count) requested by the
attending physician.
A microscope slide reading remains the reference measurement for this type
of analysis.
The use, as well as the claimed performance relate human blood and the
control sample.
If installed in medical laboratories, only staff trained and authorized laboratory
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The hematology analyzer DLB5 should be used only with dedicated reagents,
following the recommendations in this guide.
Data generated by the DLB5 is not intended to replace professional judgement in the
determination of a diagnosis or in monitoring patient therapy.
Before to use the DLB5 Analyzer, please read carefully this manual, taking
care of all important recommendations.
This operator manual contains many warnings, remarks and notes aiming to draw
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respect of these informations, the correct use of the analizer is compromised.
PLEASE KEEP AVAILABLE THIS ‘OPERATOR MANUAL’ FOR ANY FUTURE

NEEDS.
DLB5
9
INTRODUCTION
The screen shots represented on this manual can be different than the ones display on your
instrument.
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actual products characteristics and those indicated on the manual.
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DOCUMENTATION RULES
This instrument is marked with a

Information CE-mark and complies with the
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Obligation in vitro diagnostic medical de-

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Read instruction before use

Warning / Important
Manufacturer
Warning / Risk of hurt

Fragile
Shock hazard Up
General danger
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The electrical and electronic

Biological risk equipment complies with the Res-
triction of Hazardous Substances
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Direct current
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Alternating current appropriate structure.
DLB5
10
1: WARNINGS AND
CAUTIONS
DLB5
11
DLB5
12
WARNINGS AND CAUTIONS
See section 10.9 the LICENSE AGREEMENT before installing and using the instrument.
1.1. GENERAL INFORMATION
The haematology cell counter DLB5 is designed only for Srofessionnal use.
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Please use this instrument following the operator’s manual instructions.
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All operations must be disposed in accordance with local/national regulations.
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integrity of the system.
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and latest documentation. Additional information should be obtained from your distributor.
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intellectual property would incur the responsibility of the author / user.
1.2. OPERATORS
Only properly trained personnal shall use instrument.
1.3. INSTALLATION AND UTILIZATION
Refer to section 2.3.2 about Warranty Restriction linked with an incorrect installation of
the DLB5 .
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Install the instrument in a place protected from humidity, liquid splashes, high
temperature and direct sunlight.
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ÀDPPDEOH JDVHV As a place of storage of chemical products for example)
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DLB5
13
(clothing, synthetic carpets, etc.) may cause harmful electrostatic discharge and lead
to erroneous results.
DLB5 complies with emission and immunity requirements of EN 61326-2-6.
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radiation (eg RF sources intentionally unprotected), as these may interfere with proper
operation.
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commissioning the automate.
While the tubing installation, keep the tubing without distortion and folding. All the tubing
connectors must be installed manually. Don’t use any sharp or blunt tool.
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inaccurate.
When an analysis is launchHG, do not move manually the carrier at the risk
of needle damage, and injury.
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software from their distributor.
1.4. PRECAUTIONS
1.4.1. AGAINST ELECTRICAL HAZARD
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talled to a power outlet with a proper voltage. Please not that the analyzer
must be grounded (Refer to section 2.3.2)
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commissioning the automate.
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the risk of electrical shock is especially high when one’s hands are wet.
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compliant peripherals only.
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,Q WKH XQOLNHO\ HYHQW WKDW WKH LQVWUXPHQW HPLWV DQ XQXVXDO RGRU RU VPRNH
immediately turn OFF the main switch and unplug the power cable. Then
contact WKH 'LDODE VHUYLFH UHSUHVHQWDWLYH
DLB5
14
1.4.2 AGAINST BIOHAZARD
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any tasks on the instrument.
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hands.
Biological samples are potentially hazardous biological materials.

All samples, controls... must be considered potentially infectious.
Follow good laboratory practices when handling these products.

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and glasses and follow all others appropriate precautions.
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surfaces, immediately rinse the skin with large amounts of water, then follow
your laboratory’s prescribed cleaning and decontamination procedure.
The decontamination of the analyzer must be carried out in accordance with the legislation
in force in the country of use, and the instructions issued by the laboratory.
1.5. REAGENTS HANDLING
Only the Dialab brand Reagents must be used. If using other reagents, neither
the characteristics nor the functioning of the Dialab hematology analyzers can be
guaranteed
Read REAGENTS INSTRUCTION before box opening (see Appendix 10.7)
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XVHthem for any other purpose.
Please respect the expiry date and security instruction on the package.
For special safe practice, please refer to the reagents insHUWV
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DLB5
15
1.6. MAINTENANCE
For optimal performance, follow all cleaning and maintenance procedures described in
this guide
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:KHQ RSHQLQJ WKH IURQW RI WKH XQLW VHFXUHO\ HQJDJH WKH ODWFK WR SUHYHQW LW IURP
falling and causing injury.
ELECTRIC HAZARD
Switch the analyzer off and unplug the power cord from its socket before any
maintenance is carried out inside the DLB5 .
SHARP OR ANGULAR COMPONENTS HAZARD

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BIOLOGICAL HAZARD
When you carry out DLB5 maintenance, take the same precautions as the
one you take for handling biological samples.
1.7 DISPOSAL OF WASTES AND DEVICE

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local/national regulations.
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structure.
DLB5
16
2: INSTALLATION
PRESENTATION
DLB5
17
INSTALLATION-PRESENTATION
2.1 INSTALLATION
The use of DLB5 in a dry environment, especially if synthetic
materials are present (clothing, synthetic carpets, etc.) may cause
harmful electrostatic discharge and lead to erroneous results.
Do not use this device in close proximity to sources of strong
electromagnetic radiation (eg RF sources intentionally unprotected),
as these may interfere with proper operation.
2.1.1 Check equipment

When all the equipment out of the transport case as explained in the UNPACKING
INSTRUCTION, check DLB5 accessories as follows:
This manual
One external
power-supply
One thermal
printer roll paper
One power cord
One reagent tube kit

One kit of whole
blood tube
adaptors (4)
FORW¿OWHUV
Spare Reagent MS-CARD
2 keys and ADMIN MS-CARD
If the instrument was ordered with optional accessories, check their presence (external
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5HDJHQWVDQGDZDVWHFRQWDLQHUQRWLQFOXGHGLQWKHGHYLFHER[DUHDOVRUHTXLUHG
DLB5
19
2.1.2 Instrument preparation
7DNH RII WKH SURWHFWLYH ¿OP ZKLFK 3XOO RII WKH VHOIDGKHVLYH WDSH
FRYHUVWKHLQVWUXPHQW which blocks the carrier. Gently tilt the
Do not use a knife. analyzer and take the inferior part off.
1 2
3) Access inside of DLB5 . 4) Open the DLB5 .

- Unlock the machine by turning the key - Pull the hood up by opening the front
half-a-turn counterclockwise. panel.
3 4
DLB5
20
5) Steadying the hood. 6) Open position.

0RYHWKHODWFKDTXDUWHUWXUQ - Gently place the hood down onto the
latch.
5 6
3XOORIIWKHVHOIDGKHVLYHWDSHGXVW 8) Close the machine.

protector from the upper part of the /LIWWKHKRRGXSVOLJKWO\DQGPRYHWKH
dilution chambers. latch back into place.
- Gently let the hood down to its closed
position. ( Do not drop !)
- Lock the machine by turning the key
a half-a-turn clockwise.
When running, never

perform an analysis with
open hood, or results will
7 be inaccurate.
DLB5
21
2.1.3 Reagentinstallation
1) Box opening
Read REAGENTS
Verify DLB55HDJHQW reference INSTRUCTION before box
opening (see Appendix).
MS-CARD case
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1 IXUWKHUXVH
1) 2SHQWKHER[HV
7RRSHQWKHELJJHUFRQWDLQHUVSXVKLQWKHGHVLJQDWHGDUHD
DLB5
22
2) Connection of tubes to Reagent Pack
ATTENTION
- Connect caps to bottles one at a time.
- Be careful to match the correct colours as indicated on the tube coupling
and the reagent bottle (see the colour sticker on the bottle cap and the
cardboard box).
The UHDJHQWV must be kept at the same

level than the instrument. The waste
container must be kept at lower level
than the LQVWUXPHQWSUHIHUDEO\RQWKHÀ
RRU
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2) Choose the correct tube from the tube kit.
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1
4) When all tubes are connected to the reagent bottles, put theP at the left of the instrument.
5) Then connect the last tube (black coupling) to the waste container.
DLB5
23
Connection of tubes to the intrument
On the lower left side of the instrument you

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plastic plugs (+ 1 plug located at the rear
side).
These plugs are closed by protection caps
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transportation. Take off these caps by
unscrewing and keep them in a safe place
for future transportation.
Note that each plug has a colour band to
differentiate them.
It is not necessary to open the instrument
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The photo was taken this way for a better
interpretation.
Insert the reagent tubes contained in the

plastic bag into the plugs. Lock them with
a quarter turn without forcing.
The colours between the tubes

and the plugs must correspond.
Black plug for the waste is positioned

at the rear of the DLB5 .
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insert it into the card reader (see following page).
Matching colours
- Yellow : 'LDGLII (pale yellow liquid) - Orange : 'LDO\V(2 (colorless liquid)

- White : 'LDOXHQW (colorless liquid) - Green : Rinse Solution
- Blue : DLDWHUJHQW (blue liquid) - Black : Waste
DLB5
24
2.1.4 Electrical connections

The external connection ports are on the right side of the instrument. Their functions are
described below.
Motherboard V1.3 Motherboard V1.4 or higher.
MS-CARD reader
- The reagent control card must be inserted into the card
reader as shown on the right-hand photo, with the printed side
facing forward.
Wi-Fi antenna
(optional from the mother-
board V1.5)
Ethernet*
Serial port
communication #1
Ethernet (*optional),
serial (computer)
and USB B Ports
Serial port LED connexions status
USB B
communication #2 See 10.4 section
6HULDOSRUW86%%DQG(WKHUQHW DUHSURYLGHG
for computer connection.
Parallel and USB printer ports

3URYLGHGIRUH[WHUQDOSULQWHUFRQQHFWLRQ
25 pin female connector for parallel printer
type ‘Centronics’ and USB A connector.
Keyboard connector
3URYLGHGIRUH[WHUQDONH\ERDUGDQGRU
barcode reader.
6 pin PS/2 connector.
CONNECT IEC 60950-1 AND IEC 61010-1 COMPLIANT PERIPHERALS ONLY
DLB5
25
2.2 PRESENTATION
2.2.1 Front panel presentation
HOME SCREEN:
3/5 part-diff key

Indicator for next analysis
- To swich DLB5 to
- Next sequence number
3 part-diff or 5 part-diff
- Selected blood bank
Power Indicator
- Constant light if ready or
Sq 0101 Bk Control blinking if system on standby
Status indicator
READY - Shows if system can work :
Green : Ready
Orange : Priming not ready
Blue : Standby
MS-CARD Needed MS-CARD check
- Message displayed if MS-
Febr Wed 14, 2018 CARD is not inserted into
12:59:38 AM DLB5 card reader
Date/Time
- Real time on wait
screen
Menu Key
- Displays Main Menu
on the screen
Enter Key
- Validates current operation
Vertical Cursor Keys Escape/Cancel Key
Printout Key
- Select item of the menu - Cancels current operation
- Manual printing
&KDQJHVSDUDPHWHUYDOXH
Start Analysis
LQHGLW¿HOGV
- Initiates a new analysis
Paper feed key Horizontal Cursor Keys cycle
- On built-in printer - Select sub-menu
6HOHFWHGLW¿HOGVLQSDUDPHWHU
array
DLB5
26
DLB5 keyboard use
(DFKVHQVLWLYHNH\LVORFDWHGE\DOLJKWHGLFRQDNH\LVOLWZKHQHQDEOHG
A short beep acknowledges each key press.
7KHVFUHHQVKRZQRQWKHSUHYLRXVSDJHLVGLVSOD\HGZKHQVZLWFKLQJRQWKHLQVWUXPHQWDIWHU
the self-test phases. From now on it will be known as the ‘home screen’.
2.2.2 External alphanumerical keyboard use

An external keyboard can be connected and used simultaneously (PS/2 type -see section 2.1.4
IRUFRQQHFWLRQ,WSURYLGHVDGGLWLRQDOIXQFWLRQVVXFKDVWKHSRVVLELOLW\RIDQDO\VLVLGHQWL¿FDWLRQ
See other keys below.
Matching keys
DLB5 front panel External keyboard
Escape Key Escape key

3/5 part-diff F3 Function key
Start Analysis F2 Function key
Menu key F1 Function key
Enter key Return or Enter key
Cursor keys Arrow keys
Print key Print screen key
Paper feed key Insert key
Other keys
- F4 )XQFWLRQNH\DOORZVDQDO\VLVLGHQWL¿FDWLRQ
- F5 Function key allows results transmission on serial port #2.
- F8, F9 and F10 Function keys allow a quick access to Male, Female and New born blood
EDQNVUHVSHFWLYHO\WRVWDUWDQDQDO\VLVLQKXPDQXVH
1XPHULFNH\VDOORZWRHQWHUYDOXHVGLUHFWO\LQHGLWDEOHQXPHULF¿HOGV
- Tabulation and Shift+TabulationNH\VDUHXVHGWRVZLWFKIURPDQHGLWDEOH¿HOGWRDQRWKHUVDPH
function with right and left arrowNH\VUHVSHFWLYHO\
- Page up and Page downNH\VDOORZWRVZLWFKWRSUHYLRXVRUIROORZLQJGDWDWDEOH
Their use will be described in the next chapters.
DLB5
27
2.3 SETTING UP ADVICE

2.3.1 Work environment
Choose a suitable place to install the instrument.
- Put it on aÀDWULJLGVXUIDFHDQGDVVXUHFRUUHFWYHQWLODWLRQ
/HDYHDFOHDUVSDFHRQWKHOHIWVLGHRIWKHLQVWUXPHQWIRUWKHUHDJHQWSDFN
/HDYHDPLQLPXPFPVSDFHRQWKHULJKWVLGHIRUWKHH[LWRIWKHFDUULHU
- Choose a dust-free environment.
&KRRVHDFRROHQYLURQPHQW
A working environment between 20°C... 25°C (68°F... 77°F) is required to stay in

the working temperature range of the reagents. Do not install the instrument or
reagentV behind a window exposed to sunlight. Analyses performed at a
temperature outside this range could lead to incorrect results being reported.
DLB5 complies with emission and immunity requirements of EN 61326-2- 6.

Do not use this device in close proximity to sources of strong
electromagnetic radiation (eg RF sources intentionally unprotected), as these
may interfere with proper operation.
It is advisable to carry out an assessment of the electromagnetic
2.3.2 Power connection

Use a ground connection plugged into the mains. A ground plug is essential for the instrument
WRZRUNHI¿FLHQWO\
Perturbed mains or bad ground connection could lead to incorrect results.
IMPORTANT
- The absence of ground connection is DANGEROUS for user safety and for the
intrument electronic parts. Any deterioration to parts of the DLB5 due to a non-
compliant connection will invalidate the warranty on the instrument.
- The socket (see picture #1) is used as main disconnect device and must be easily
accessible.
1- External power supply 1

Input: 100-240 V / 50-60 Hz 2 A
$XWRPDWLFFRPPXWDWLRQYROWDJH
Output: 24.0 V 6.25A
2- Internal power supply
Input: 24.0 V 6.25A

2 Fuse: F 7 A-125 V
Power switch (z ON)

DLB5
28
3: FIRST
UTILIZATION
DLB5
29
CAUTION
BEFORE SWITCHING ON THE DLB5 ,
CHECK THAT THE WASTE CONTAINER IS
CORRECTLY CONNECTED TO THE BLACK PLUG
LOCATED AT THE REAR OF THE DEVICE.
The rest of reagents, detergents as well as all the waste must be eliminated in
the respect of the legal instructions in effect in the country.
FIRST UTILIZATION
3.1 POWER UP
:KHQ DOO HOHFWULFDO DQG ÀXLGLF UHDJHQWV FRQQHFWLRQV DUH PDGH DFFRUGLQJ WR WKH SUHYLRXV
instructions, insert the MS-CARD into the card reader, with the printed side facing forward.
The instrument can be switched on by pressing the main power switch located at the rear of
the instrument.
3.1.1 Self-tests
:KHQSRZHULVRQWKHLQVWUXPHQWVWDUWVVHYHUDOLQWHUQDOFRQWUROVWRWHVWWKDWWKHHOHFWULFDODQG
mechanical functions are correct.
This step is characterized by a succession of different sound frequency signals and a cyclic
lighting of the LED on the front panel.
This step is followed by the setting of the diluent piston, sample needle and carrier.
3.1.2 Priming reagents

When the self-test step has been carried out successfully,
PRIMING the instrument knows that reagents are missing (this is the
¿UVWLQVWDOODWLRQ,WZLOOUXQWKHQDQDXWRPDWLFSULPLQJF\FOH
During this process a message as shown on the left is
displayed.
7KLVSURFHVVFDQWDNHVHYHUDOPLQXWHVWR¿OODOOWXEHVZLWK
Please wait... reagent and purge all residual air bubbles.
Cycle in progress
- Abnormal priming of reagents

PRIMING
:KHQLQVWDOOLQJWKHLQVWUXPHQWIRUWKH¿UVWWLPHRUFKDQJLQJ
Priming LYSE error the reagent pack, the analyzer may not be fully primed.
Check levels A message displays to indicate which reagent has not
been primed.
Let the instrument end the draining cycles until the home
screen displays.
The priming will end automatically afterwards.
- Skipping priming step
,ISULPLQJRIUHDJHQWVLVQRWQHFHVVDU\DWWKHSRZHURQLHWRFRQVXOWSUHYLRXVDQDO\VLVUHVXOWV
hold the Cancel key until the home screen is displayed.
7KH'/%DOZD\VSULPHVWKH'LDO\V(2HYHQLISDUWGLIILVVHOHFWHG
If 5 part-diff is selected before priming and if there is no 'LDO\V(2, the DLB5 asks
the operator to switch to 3 part-diff.
DLB5
31
FIRST UTILIZATION
3.2 PRINTOUT
3.2.1 The printers
The internal thermal printer.
7KHGHYLFHLVHTXLSSHGZLWKDVPDOOEXLOWLQWKHUPDOSULQWHUFROXPQVGRWVWKDWDOORZV
the printing of the analysis results on a ticket and also statistical summaries.
An external printer (parallel or USB) can also be connected for printing on personalized paper.
3.2.2 Printer paper installation

5HPRYHWKHDGKHVLYHWDJIURPWKHSDSHUUROO
/LIWWKHSULQWHUFRYHUWRJHWDFFHVVWRWKHSDSHUFRPSDUWPHQW
,QVHUWWKHSDSHUUROOLQLWVFRPSDUWPHQWOHDYLQJWKHHQGRIWKHUROORXWVLGH
)LUPO\SXVKWKHFRYHUGRZQZDUGXQWLOLWFOLFNVLQSODFH
1 2
3 4
Advice
- Store the paper rolls away from heat, light and humidity.
- A red mark on the paper indicates the imminent end of the roll. When it appears, check your
stock and order immediately if necessary.
Ref rolls : +

Item : Thermal paper rollx
DLB5
32
FIRST UTILIZATION
3.2.3 Example of ticket

7KHWLFNHWSULQWHGRQEXLOWLQSULQWHUFDQWDNHGLIIHUHQWDVSHFWVDFFRUGLQJWRWKHFRQ¿JXUDWLRQ
setup (See Printout Menu chapter). The most common representation is reproduced below (3
part-diff).
Personalized heading
&RQ¿JXUDEOH
Analysis information
- Date / Time
- Selected blood bank
- Sequence number
VSHFL¿FLWHPV
- Entered with DLB5 keyboard or an external alphanumeric
keyboard (or code bar reader for PATIENT)
Haematology results
Min/max alarm
- Normal ranges can be enabled from
Printout Menu and printed on the
following line
Cursors
- Mono Start / End
Histograms
- Printout can be disabled
from Printout Menu.
DLB5
33
FIRST UTILIZATION
DLB5
34
FIRST UTILIZATION
3.3 RUN AN ANALYSIS

3.3.1 General advice
+XPDQ %LRORJLFDO ÀXLGV DUH SRWHQWLDOO\ KD]DUGRXV ELRORJLFDO PDWHULDOV
This material should be handled as if capable of transmitting infectious
diseases as Hepatite B, Hepatite C and HIV.
Follow good Laboratory practices when handling these products.
Take appropriate precautions when handling all laboratory samples to
prevent skin contact (wearing coat, gloves, goggles,...), ingestion, or inhalation.
IMPORTANT: Before running an analysis, read METHOD LIMITATION -

INTERFERENCES chapter (Appendix 6).
IMPORTANT: Respect pre-analytical phases during sample preparation as well

as applicable quality procedures within the laboratory.
IMPORTANT: At each startup of the analyzer and / or after reagent priming,

SHUIRUPWZRDQDO\]HVWKH¿UVWZLWKRXWSXWWLQJDWXEHDQGWKHVHFRQGZLWKD
control blood or a blood tube from the series to be analyzed.
,03257$17&KHFNSHUIHFWO\WKHLGHQWL¿FDWLRQRIWKHEORRGWXEHDQGLWV
LGHQWL¿FDWLRQLQWKHDQDO\]HU
Blood sampling
The tested and recommended anticoagulant is K3 EDTA.
Other anticoagulants can generate inaccurate results.
Sampling tubes
'LIIHUHQWVL]HGEORRGVDPSOLQJWXEHVFDQEHXVHGRQWKH'/%'LIIHUHQWDGDSWHUVDUHDYDLODEOH
to adjust the right size of tube.
,03257$17,WLVQHFHVVDU\WR¿OOWKHWXEHZLWKWKHFRUUHFWEORRGTXDQWLW\
If the amount of blood in the tube does not reach the necessary quantity, the
3-part and 5-part -differential analysis will not be stable and the platelet count
will be disrupted. Fill the tube to the indicated line in order to have a correct
proportion between the blood and the anticoagulant.
Homogenization
%HIRUHDEORRGDQDO\VLVWKHEORRGPXVWEHJHQWO\PL[HG7KLVVWHSLVYHU\LPSRUWDQWDQGFDQ
sometimes require two minutes for a perfect homogenization.
0L[WKHZKROHEORRGE\VORZO\LQYHUWLQJWXEH
Thorough mixing is recommended when:
- Blood has just been taken sampled from the patient (less than 20 minutes).
- Blood is sedimented.
DLB5
35
FIRST UTILIZATION
3.3.2 First analysis after installation
Function of the keys
7KHPRGL¿DEOH¿HOGLVLQLQYHUVHYLGHRZKLWHFKDUDFWHUVRQEODFNEDFNJURXQG
7RFKDQJHIURPRQH¿HOGWRDQRWKHU Right and Left arrows

7RFKDQJHWKHYDOXHLQVLGHWKHVHOHFWHG¿HOG High and Down arrows
3- To abort the current action : Escape key
7RYDOLGDWHDQGTXLWWKHFXUUHQWIXQFWLRQ Enter key
5- To initiate an analysis cycle : Start analysis Key
6- To select 3 or 5 part-diff mode : 3/5 part-diff Key
,IQHFHVVDU\¿UVWSUHVVSDUWGLII NH\WRVHOHFWRUSDUWGLIIPRGH
Press Analysis key on built-in keyboard

(or F2 Function key on external keyboard)
7KH'/%VFUHHQGLVSOD\VDVEHORZSDUWGLIIWRFRQ¿UPWKHSDUDPHWHUVRIWKHDQDO\VLVWRUXQ
Part-diff mode (3/5)
Sequence number
- For this analysis
3 PART ANALYSIS Error message
- Displayed only if NO MS-CARD
Sq : 0101
inserted into card reader
Insert MS-CARD
Selected line
ANALYSIS Free #1 - Enter Key runs analysis
QUALITY CT Current blood bank
- Parameters used for this analysis
Run a quality control analysis
/HYHORIFRQWUROEORRGLVGLVSOD\HGZKHQWKLVLWHPLVVHOHFWHG
DLB5
36
FIRST UTILIZATION
3.3.3 Start screen interpretation

If the DLB5 asks for a reagent control card, insert it into the card reader. The start test will be
denied until a MS-CARD has been recognized.
- The sequence number

,WLVLQFUHDVHGDIWHUHDFKYDOLGDQDO\VLVLVVWRUHG7KHUHLVDQRUGHUQXPEHUIRUWKH%ORRG$QDO\VLV
and another for the Quality Control Analysis.
- Selected Menu line

$VHOHFWHGOLQHDSSHDUVKLJKOLJKWHGLQFRORXU7KLVOLQHZLOOEHDFWLYDWHGZKHQSUHVVLQJEnter key.
- The Blood Bank

Determines the parameters used for the next analysis.
To change the bank, please follow these instructions:
- Press the Right arrow key: the blood bank name is highlighted to indicate that it is selected.
- Press the Up arrow or Down arrow NH\ QDPHV RI WKH RWKHU DYDLODEOH EDQNV DUH
sequentially displayed.
- Once the desired blood bank is displayed, press Left arrow or Enter key.
The ANALYSIS or BANK line of the menu is then re-selected.
- To run a test, the ANALYSIS line must be selected. If the BANK line is selected, press the Up
arrow key to change the selection.
If an external keyboard is connected to the instrument, use F8, F9 or F10 Function

NH\VWRVNLSWKLVVFUHHQ7KHLGHQWL¿FDWLRQVFUHHQGHVFULEHGRQQH[WSDJHZLOOEHWKHQ
GLVSOD\HGZLWKWKH0DOH)HPDOHRU1HZERUQEORRGEDQNVUHVSHFWLYHO\VHOHFWHG
Cancel key (or Escape on external keyboard)

This key allows a return to the home screen, and generally cancels the current operation
WRUHWXUQWRWKHSUHYLRXVRQH
Enter key (or Return/Enter on external keyboard)

3UHVVWKLVNH\WRFRQ¿UP¿UVWDQDO\VLVVWDUW
Comment
Sq 0101 Bk Free #1 If the reagent priming has not been completed correctly
during the first power on, the opposite message is
Priming reagents displayed.
error Press Enter NH\WR¿QLVKSULPLQJEHIRUHWKHDQDO\VLV
Validate to This an intermediate step before the start of the counting
Restart priming described hereafter.
Cancel analysis This message can also display if no analysis has been
done for 6 hours.
DLB5
37
FIRST UTILIZATION
,GHQWL¿FDWLRQ
7KHDQDO\VLVYDOLGDWLRQDFWLYDWHVWZRDFWLRQV
7KHFDUULHUPRYHVWRWKHULJKWVLGHRIWKHLQVWUXPHQW7KLVDOORZVWKHXVHUWRSXWWKHZKROH
blood sample tube into the adapter.
Sq 0101 Bk Free #1 2) The screen opposite is displayed to identify the analysis.

$ ILOH QXPEHU LV JLYHQ E\ GHIDXOW WKH FXUVRU LV WKHQ
FILE positioned on the last character. This number (1 by default
20180203-001 ZKHQXVLQJWKHLQVWUXPHQWIRUWKH¿UVWWLPHGHSHQGVRQ
PATIENT the current date and number entered at the last analysis.
The number starts with the current date (e.g.
COMMENT 20100203: YYYYMMDD) and ends with a number of 3
digits (e.g. 001) up to 999.
0RGL¿FDWLRQZLWKWKHEXLOWLQNH\ERDUG
Up Arrow or Down Arrow keys allow scrolling of the letters up or down. Right Arrow
or Left Arrow keys allow to shift from one letter to another along the line.
0RGL¿FDWLRQZLWKDQH[WHUQDONH\ERDUG
For an easier use. Just type the letters and correct with the correction key.
Note:,WLVUHFRPPHQGHGWRHQWHUQXPHULFDOFKDUDFWHUVIRUWKH¿OHQXPEHULGHQWL¿FDWLRQPDWFK
search with hematology is only done on numbers.
:KHQWKH¿OHQXPEHULVFRUUHFWWZRSURFHGXUHVDUHSRVVLEOH
1) Use the Enter NH\WRPRYHWRWKHQH[WOLQHDQGHQWHUGDWDZLWKDPD[LPXPRIFKDUDFWHUV
per line.
With an external keyboard, the Page up key allows

Sq 0101 Bk Free #1 PRYHPHQWWRWKHXSSHUOLQH
If the cursor is on the last line the Enter key displays
Place whole blood WKHIROORZLQJVFUHHQWRFRQ¿UPWKHEORRGVDPSOLQJWXEH
sampling tube then is present.
Validate... 2) You can use the Analysis key (or F2 on the external
NH\ERDUGRQWKHLGHQWL¿FDWLRQVFUHHQDWDQ\SRLQWLQRUGHU
to start the analysis cycle immediately.
WARNING
The blood sampling tube must be in the carrier before using this key.
Never try to remove the sampling tube during the dilution cycle, this could
generate a carrier translation error.
DLB5
38
FIRST UTILIZATION
Setting the sampling tube
Choose the correct sized tube adapter for the

sampling tube.
Insert this adapter into the circular stand of the carrier.
Do not move the carrier during the handling and
positioning of the adapter and tube.
WARNING NEEDLE : Risk of hurt. A

pictogram indicates the danger. Rise and fall
movHments of the needle when the analysis
begins.
When an analysis is launchHG, do not

move manually the carrier at the risk of
needle damage.
Take the cap off the sampling tube and insert it into
the adapter.
When the message asking for the sampling tube is

displayed, press Enter key or Analysis key (F2
key with an external keyboard).
Using a Barcode reader
:KHQXVLQJDEDUFRGHUHDGHUZKLOHWKHLQVWUXPHQWLVRQWKHZDLWVFUHHQWKHLGHQWL¿FDWLRQVFUHHQ
is automatically displayed without using the Analysis key. Data is then automatically listed under
the PATIENT item .
7KHEDUFRGHUHDGHUFDQDOVREHXVHGDWDQ\WLPH:KHQWKHLGHQWL¿FDWLRQVFUHHQLVGLVSOD\HG
during the analysis, or when the results are displayed.
Each of these 3 lines can admit 20 characters maximum.
7KHLGHQWL¿FDWLRQVFUHHQZLOODSSHDULQWKHUHVXOWVVFUHHQVGHVFULEHGRQWKHQH[WSDJH
When this screen is displayed following the results screen, press Up Arrow key (or F4
function key on external keyboard) to change into edit mode (display mode does not show the
highlighted edit cursor).
7KHLGHQWL¿FDWLRQZLOOEHORVWLIWKHDQDO\VLVLVLQWHUUXSWHGEHFDXVHRIDQDQRPDO\HJ
missing reagent).
When the analysis cycle starts, all the dilution and counting cycles are automatic.
The main steps of the cycle are displayed on the screen.
DLB5
39
FIRST UTILIZATION
3.3.5 Results
Haematology results are displayed across 4 or 5 screens. To switch between screens, press
Right arrow or Left arrow NH\V7KHODVWVFUHHQ,GHQWL¿FDWLRQLVDOVRGLVSOD\HGWREH
PRGL¿HGLIUHTXLUHG
%HORZDQH[DPSOHRIWKHUHVXOWVFUHHQVDQGLGHQWL¿FDWLRQVFUHHQ
1st screen: Main results 2nd screen: THR Results
Sq: 0101 02/03 12:59PM Sq 0101 Bk Free #1

WBC 12.22 m/mm3 THR
RBC 4.73 M/mm3
Hct 43.6 %
MCV À
MPV :À
Hb 14.2 g/dl
Pct : 0.17 %
THR 244 m/mm3
PDW : ? 6.2
3rd screen: RBC Results 4th screen: WBC Results
Sq 0101 Bk Free #1 Sq 0101 Bk Free #1

RBC WBC
MCH : 30.0 pg LYM: 35.3 % 4.31 #

MCHC : 32.5 g/dl MON: 4.1 % 0.50 #
RDW : 10.5 NEU: 58.1 % 7.10 #
5th screen: EO Results Last screen: Patient ID

(Only 5 part-diff)
Sq 0101 Bk Free #1 IDENTIFICATION
EO FILE
20100203-001
PATIENT
EO : 2.3 % 0.28#
BA : 0.2 % COMMENT
For 5 part-diff :
:KHQ&RQWUROEORRG%DQNLVVHOHFWHG%$SDUDPHWHULVQRWSURFHVVHGVRWKHYDOXHLV
,WLVUHFRPPHQGHGWRNHHSWKHVFDQLPSUHVVLRQVIRUDUFKLYDOSXUSRVHV
DLB5
40
FIRST UTILIZATION
,QDGGLWLRQWRWKHTXDQWLWDWLYHUHVXOWVDQGKLVWRJUDPVRWKHULQGLFDWLRQVFDQDSSHDURQWKHVHVFUHHQV
3.3.5.1 Normal range alarm

If a test result is out of the normal range, an arrow is displayed to indicate if high or low (e.g.
PDW UHVXOWRQWKHVHFRQGVFUHHQDERYH
3.3.5.2 Counting Alarms

& Mark : A blockage has been detected during counting.
# Mark : A blockage has been detected just before counting.
@ Mark : homogeneity error has been detected during counting.
T Mark : Thalassemia possibility. (Only on Male, Female and New born banks).
? Mark : result is 0 due to impossibility of interpretation.
R Mark : result rejected caused by a bad counting parameter
- On lines RBC + MCV + Hct + Hb => MCHC too high
- On line WBC => 3 Part-diff error (Reminding of L or I alarm described below)
L Mark : On lines LYM + MON + GRA (3 part-diff):
On lines LYM + MON + NEU + EOS + BAS (5 part-diff)
WBC counting error => Lympho interferences
Caused by Platelet clumps or Nucleated RBC
M Mark : On lines LYM + MON + NEU (GRA): WBC counting error => monocytes too high
- Too high quantity of Blasts or haemolyzed blood (too old) or 3 part-diff not well calibrated
I Mark : On lines LYM + MON + NEU (GRA): WBC counting error => immature cells
&DQEHÀDJJHGDOVRZKHQSDUWGLIILVQRWZHOOFDOLEUDWHGRUEORRGDQDO\]HGLQDQLQFRUUHFWEDQN
E Mark : error generally caused by hardware (e.g. photometer out of order).
$QHZDQDO\VLVRIWKHVDPSOHVKRXOGEHFRQVLGHUHGLQWKHHYHQWRIDFRXQWLQJDODUP
In case of abnormal Leukocyte Formula or any associated parameter outside the
range of normality (eg GB), a slide read under the microscope must be made if the
formula is required.
In case «the basophilic polynuclear cells» is one of the essential parameters of the
LQYHVWLJDWLRQRQWKHIRUPXODDVOLGHPXVWLPSHUDWLYHO\EHPDGHIRUUHDGLQJXQGHUWKH
microscope. The result rendered by the apparatus being related to a calculation on the
SRO\QXFOHDUDORQHZLWKRXWFRQVLGHUDWLRQRIRWKHUEDVRSKLOVFHOOVLWLVRQO\LQGLFDWLYH
8SGDWHLGHQWL¿FDWLRQVFUHHQ
7KH&200(17OLQHRQWKH,GHQWL¿FDWLRQVFUHHQFDQEHFKDQJHGZKHQWKLVVFUHHQLVGLVSOD\HG
by holding the Up Arrow key (or F4 Function key on an external keyboard).
3.3.5.4 Send results to a computer

Results can be transferred manually to a computer by pressing the Up Arrow key (or F5
Function key on an external keyboard) when the 1st screen is displayed. Refer to Export Data
chapter for more details.
2QO\WKHDQDO\VLVUHVXOWVDYDLODEOHRQWKHPDFKLQHDUHYDOLG6FUHHQDQGSULQWHULQWHJUDWHG
In case of power failure during a transfer of results, renew the data export.
DLB5
41
FIRST UTILIZATION
3.3.5.5 Results printout
Press this key to print results (or Print screen on the external keyboard).
Results are printed on the external printer if this is on-line. If no external printer is
DYDLODEOHDWLFNHWLVSULQWHGRQEXLOWLQSULQWHU3ULQWLQJUHVXOWVFDQEHPRUHGHWDLOHGWKDQ
displayed about alarm marks.
On the external printer, the normal range alarms for the Leukocyte formula are done
based on the results in % and are AUTOMATICALLY REPORTED on the results in
DEVROXWHYDOXHV
3.3.5.6 Dilution x 2
,IWKH:%&UHVXOWLVYHU\KLJKXSSHUWKDQPPP3), the DLB5 displays the following warning

message:
ATTENTION
Very high WBC result

provided without accuracy
It is advisable to
repeat the analysis
with dilution factor X 2
Then press any key to get the main results screen.

When you quit the result screens, with Escape/Cancel Key , the following screen is displayed :
ATTENTION
If you answer NO , DLB5 goes back to the home screen.
Repeat the analysis with
a dilution factor X2?
If you answer YES '/%JRHVEDFNWRWKHLGHQWL¿FDWLRQ
screen with the proposed
YES Dilution X 2WH[WLQWKHFRPPHQW¿HOGVHHVFUHHQEHORZ
NO
Sq 0102 Bk Free #1
At this step, if you continue to run the analysis, the
FILE predilution factor is automatically multiplied by 2.
20180203-002 The patient results will correspond to the original
PATIENT concentrations.
COMMENT
Dilution X 2
DLB5
42
4: CONFIGURATION
DLB5
43
CONFIGURATION
6\VWHPFRQ¿JXUDWLRQLVSHUIRUPHGDFURVVGLIIHUHQWMenus.
Press MenuNH\ZKHQYDOLG/('RQRUF1 function key on an external keyboard.
4.1 MAIN MENU

)URPWKHYHUVLRQWKH'/%PDQDJHVZRUNLQJ
PRGHV8VHUDQG$GPLQLVWUDWRU)RUWKHSUHYLRXVYHUVLRQV
RQO\WKHDGPLQLVWUDWRUPRGHLVDYDLODEOH6HHVHFWLRQV
The content of the menu depends on the current operator mode.

By default, the analyser works in user mode.
To change of mode, insert the ADMIN card (see section 6.4).
User mode :
The main menu is displayed with the 1st line selected by

MANAGEMENT
default. The items order corresponds to the frequency of
RECALL ANALYSIS use.
SYSTEM To select another item, use keyboard Up arrow or Down
TECHNIC
STANDBY arrow keys.
CLEAR ANALYSIS Example: to select the SYSTEM item, press Down arrow
key twice. When the SYSTEM line is selected, press Enter
key to call the sub-menu.
Administrator mode :
MANAGEMENT
RECALL ANALYSIS
The main menu has an additional line :
PARAMETERS
SYSTEM
PARAMETERS
PRIME/RINSE
STANDBY
CLEAR ANALYSES
DLB5
45
CONFIGURATION
4.2 RECALL ANALYSIS MENU

Allows the recall of an analysis from the last 100 or 250* last analyses stored in the memory.
* DLB5 manage up to 250 analyses, only if it is equipped

ZLWKDPRWKHUERDUGUHYRUKLJKHU
7KHVWDQDO\VLVGLVSOD\HGLVWKH¿OHQXPEHURQO\HQGLQJFKDUDFWHUVRIWKH)LOH1DPHGLVSOD\HG
of the last analysis performed, followed by the date (Day/Month or Month/Day according to the
ODQJXDJHWLPHKRUKIRUPDWDFFRUGLQJWRWKHFRQ¿JXUDWLRQDQGFKDUDFWHUVIURPWKH
blood bank reference.
Up arrow or Down arrow keys allows the selection

SELECT ANALYSIS
of the requested analysis. When the selected line
00203-003 02/03 2:59P Ct reaches the end of an array, the next one is displayed.
00203-002 02/03 2:50P Ct With an external keyboard, Page up or Page down keys
00203-001 02/03 2:44P Ct allow a rapid change of screen.
00202-002 02/02 9:39A Hm
00202-001 02/02 7:48A Hm
7KH¿OHQXPEHUYDOLGDWLRQGLVSOD\VUHVXOWVFUHHQVRIWKH
00201-001 02/01 7:32A Fm
VHOHFWHGDQDO\VLVVHHSUHYLRXVFKDSWHU
Blood bank abbreviations:

5HIHUWRVHFWLRQWRNQRZZKHQWKHVHEDQNVDUHDYDLODEOH
Hm Male
Fm Female
Nn New born
Ct Control
L1 Free # 1
L2 Free # 2
DLB5
46
CONFIGURATION
4.3 PARAMETERS MENU
)URPWKHYHUVLRQWKHDFFHVVRIWKLVPHQXUHTXLUHGWKHLQVHUWLRQRI
an MS-CARD ADMIN on the DLB5 (see section 4.1 and 6.4).
This menu is called from the second line of the main menu (See section 6.4).
SET PARAMETERS
MODIFY Free #1
EDIT NAME
CALIBRATION
QUALITY CT
USER
When the MODIFY or EDIT NAME lines are selected, the name of the current blood bank is
displayed on the right. This is the bank affected if one of these lines is chosen.
To change the blood bank, select the name with the Right arrow key and choose among
WKHDYDLODEOHEDQNVZLWKWKHUp arrow or Down arrow keys.
Use then the Left arrow or Enter key to re-select MODIFY.
DLB5
47
CONFIGURATION
4.3.1 EDIT NAME BLOOD BANK

7KHQDPHRIDEORRGEDQNFDQEHPRGL¿HGE\WKLVLWHP
,WLVRQO\DYDLODEOHIRU)UHHDQG)UHHEDQNVVHHUHDJHQW06&$5'FKDSWHU
For the editing name process, see the USER sub-menu chapter.
Comment: Each blood bank stocks its name in 3 languages. Calling the EDIT NAME menu will
only change the name into the current language. Change the language temporarily and repeat
WKHPRGL¿FDWLRQXQGHURWKHUODQJXDJHVLIQHHGHG
4.3.2 MODIFY BLOOD BANK

IMPORTANT
7KH ¿UVW VFUHHQV ZKLFK LQFOXGH WKH FRXQWLQJ DQG FDOLEUDWLRQ SDUDPHWHUV
PXVW be changed with great care and if possible with the help of a Dialab
technician. If incorrect data is entered then the analysis results will be inaccurate.
:KHQYDOLGDWLQJWKH02',)<OLQHDVHULHVRIVFUHHQVLQFOXGLQJDOOWKHSDUDPHWHUVRIWKHVHOHFWHG
blood bank (e.g. Free #1) is displayed.
Each screen can be accessed with Page Up/Down keys on the external keyboard.
7KHFRQWHQWZLOOEHH[SODLQHGLQGHWDLOLQWKHHYHQWWKDWDWHFKQLFLDQKDVWRJLYHPRGL¿FDWLRQ
instruction.
Note: It is necessary to quit a screen by the Enter NH\VRWKDWPRGL¿HGGDWDFDQEHUHFRUGHG
1st screen: Cell discriminator
BANK : Free #1 Hi.T: High dimensional threshold. Larger-sized cells are

THR RBC WBC EO rejected.
Hi.T 240 220 240 150 LowT: Low dimensional threshold. Smaller-sized cells are
LowT 018 020 020 035 rejected.
MinW 016 020 020 026 MinW: Minimal cell width. Smaller- width cells are rejected.
MaxW 060 150 200 100 MaxW: Maximal cell width. Bigger- width cells are rejected.
Time 10 10 10 04 Time: Counting time in seconds.
DLB5
48
CONFIGURATION
2nd screen: Cell discriminator
Gain:$PSOL¿HUFRHI¿FLHQW
BANK : Free #1
LowF:/RZ¿OWHUFHOO
THR RBC WBC EO Hi.F:+LJK¿OWHUFHOO
Gain 240 180 170 200 H/W F:+HLJKW:LGWK¿OWHUFHOO
LowF 80 0 80 70 Probe:3UREHYROWDJHLQYROW
Hi.F 80 0 80 70
H/W F ; ;
Probe 15.0 14.0 14.0
3rd screen: Dilution Parameters for 3-Part-Diff
Prediluted: 1st Dilution rate (WBC dilution)

BANK : Free #1
Dilution #2: 2nd dilution rate (RBC / THR dilution)
3 PART. PARAMETERS Lyse: Lyse quantity in ml
Prediluted 500 Mono Start: Monocytes start cursor **
Dilution #2 135 Mono End: Monocytes end cursor **
Lyse 0.80
Mono Start 074 ** See Appendix 3 ‘3 PART-DIFF. CALIBRATION’
Mono End 087
4th screen: Dilution Parameters for Eo counting
EO Dilution: Blood Dilution rate

BANK : Free #1
Lyse EO Dil.: Quantity of diluent used to dilute EO Lyse
EO PARAMETERS Reaction delay: Wait time in seconds before EO counting
EO Dilution 150
Lyse EO Dil. 1
Reaction delay 00
WKVFUHHQ&RUUHFWLRQFRHI¿FLHQWV
&RUUHFWLRQ FRHI¿FLHQWV IRU WKH EORRG EDQN XVHG IRU WKH

BANK : Free #1
calibration function.
Coef THR 1.00 Note: For the Control Blood bank, this screen does not
Coef RBC 1.00 appear. The calibration is done only by general calibration.
Coef WBC 1.00
Coef Hb 1.00
Coef MCV 1.00
9DOXHVVKRZQLQWKLVPDQXDODUHRQO\JLYHQDV
Coef EO 1.00
an example and may differ from the actual bank
GDWDLQWKHGHYLFH
DLB5
49
CONFIGURATION
6 to 15th screen: Normal range
1RUPDOUDQJHDOORZVWKHÀDJJLQJRIWKUHVKROGDODUPVLQ
BANK : Free #1
DQDO\VLVUHVXOWV:HKDYH
Std Min Max 3 haematology screens (units are listed in export data
WBC 001.6 025.4 chapter according to the Hb Unit selected)
Lym% 21.0 67.0 VFUHHQVIRUUHVHUYHGXVH
Mon% 00.5 06.5
Neu% 31.0 78.5 Note: alarms and normal range printouts are only taken into
Eo% 01.0 08.0
DFFRXQWLIDQRQ]HURYDOXHLVHQWHUHGLQWRWKHSDUDPHWHU
Max column.
Reminder of selected Unit
3UHYLRXVO\GHVFULEHGFRHI¿FLHQWVFDQEHSULQWHGRQEXLOWLQSULQWHULQIRXUJURXSV
%DQNFRXQWLQJSDUDPHWHUVDQGFRUUHFWLRQFRHI¿FLHQWV
2) Bank haematology normal range
2WKHUEDQNQRUPDOUDQJHUHVHUYHGXVH
,QVWUXPHQWFDOLEUDWLRQFRHI¿FLHQWV
Press the Print key (Print screen key on external keyboard) when one of these
DERYHJURXSLVGLVSOD\HGRQWKHVFUHHQ
4.3.3 CALIBRATION
,QVWUXPHQWFDOLEUDWLRQFRHI¿FLHQWV
CALIBRATION
Coef THR 1.00
Coef RBC 1.00
Coef WBC 1.00
Coef Hb 1.00
Coef MCV 1.00
Coef MPV 1.00
7KHVHOHFWLRQRIWKLVOLQHIURPWKH3DUDPHWHUV0HQXGLVSOD\V¿UVWDFRHI¿FLHQWFRUUHFWLRQVFUHHQ
This screen is the same as the 5th parameters screen of the blood bank except the last line
ZKLFKLVWKH039FRHI¿FLHQW7KHRQO\GLIIHUHQFHLVWKDWLWFRQFHUQVWKHJHQHUDOFDOLEUDWLRQRI
the instrument.
It is not used to correct cell structure differences according to the different types of blood but only
to adjust mechanical disparity between the different instruments (size of the counting aperture
for example).
The instrument calibration must only be performed with control blood.
Refer to chapter 7.2 for automatic calibration.
When this screen is displayed, it can be printed (only on the built-in printer) by pressing
the Print Key (Print screen key on external keyboard).
DLB5
50
CONFIGURATION
6PRRWKLQJFRHI¿FLHQWV
,QRUGHUWRLPSURYHWKHUHSUHVHQWDWLRQDQGFDOFXODWLRQVFDUULHGRXWRQWKHGLVWULEXWLRQKLVWRJUDPV
VPRRWKLQJLVSHUIRUPHGWKHFKDUDFWHULVWLFVRIZKLFKDUHGH¿QHGLQWKHVFUHHQEHORZ7KHVH
parameters are the same for all blood banks and must not be changed.
SMOOTHING Smth.:1XPEHURIVXFFHVVLYHVPRRWKLQJVWR

THR RBC WBC EO Range:1XPEHURIVPRRWKHGSRLQWVRUUHVSHFWLYHO\
Smth 1 1 2 1 matching to 3, 5 or 7 points width).
Range 2 2 2 2
Caution: Parameters above are used to calculate
Disp. 5 3 3 3
results. Changing one of this parameters could
Exp. ; ; lead to de-calibration of some tests.
Disp.:1XPEHURIVXFFHVVLYHVPRRWKLQJVXVHGWREXLOGGLVSOD\HGRUSULQWHGKLVWRJUDPV : no
smoothing to 3/5: maximum smoothing).
Exp.: Unchecked for a normal displayed or printed histogram, checked for a height-expanded
histogram. The expansion will only be applied if it is necessary.
4.3.4 USER HEADING

Displays a succession of 4 screens which allow personalized headings to be entered and printed
at the beginning of the analysis results.
(DFKVFUHHQHGLWVDOLQHRIFKDUDFWHUVPD[LPXPDQGDOORZVWKHMXVWL¿FDWLRQRIWKHWH[WRQ
WKHUHSRUWOHIWRUFHQWHUMXVWL¿HG
The heading is a maximum of 4 lines-long.
An empty line will not be printed.
MODIFY LINE #1 1) Edit with external alphanumeric keyboard:

Type the text with a maximum of 40 characters. The cursor
will hold the last position if the end of the line is reached.
DLB5 CELL COUNTER
Use the Enter NH\WRVHOHFW-867,)<DQGHYHQWXDOO\
modify with the Up arrow or Down arrow keys.
Use the Enter key again to switch to the screen for the
JUSTIFY CENTER following line. (Line number appears in the title bar).
2) Edit with built-in keyboard:

Use Up arrow or Down arrow keys to change the selected character then jump to the
next letter using the Right arrow key.
To cancel any character, select the space character.
:KHQWKHOLQHLV¿QLVKHGSURFHHGE\VHOHFWLQJWKHEnter key to choose JUSTIFY.
DLB5
51
CONFIGURATION
4.3.5 QUALITY CONTROL

7KLVPHQXSHUPLWVWKHVHWWLQJRIFRQWUROEORRGSDUDPHWHUVRQWKUHHOHYHOVWRSHUIRUPTXDOLW\
control tests.
When the menu item QUALITY CT is selected, the control

SET PARAMETERS EORRG OHYHO WR EH LGHQWL¿HG LV GLVSOD\HG RQ WKH ULJKW 7R
MODIFY change it, use the Right arrow key to select it and
EDIT NAME FKRRVHWKHGHVLUHGOHYHOE\PHDQVRIWKHUp arrow or
CALIBRATION
QUALITY CT LOW Down arrow NH\VWKHQYDOLGDWH .
USER
$ EORRG FRQWURO 06&DUG LV DYDLODEOH WR VHW

automatically all the parameters. See section 6.3.
&RQWUROEORRGLGHQWL¿FDWLRQ
CONTROL LOW Use the Enter NH\ WR JHW WKLV ¿UVW VFUHHQ ,W DOORZV
LGHQWL¿FDWLRQRIWKHFRQWUROEORRGDWWKHUHTXLUHGOHYHO
,IQRWGH¿QHGSUHYLRXVO\WKHGDWHE\GHIDXOWLVGLVSOD\HG
LOT NUMBER as the current date.
000 00 It is necessary to enter the information on this screen to
EXPIRY DATE
perform a quality control analysis.
06 23 2018
Note: LOT NUMBER is always numeric and cannot be
alphanumeric.
Control blood values
CONTROL LOW 7KLV VHFRQG VFUHHQ SHUPLWV WR HQWHU WKH YDOXHV IRU WKH
Std ASSAY 2SD FRQWUROEORRGDWWKLVOHYHO
WBC 02.4 0.5
RBC 2.48 0.2 'HIDXOWYDOXHVPXVWEHFRUUHFWHGLQDFFRUGDQFHZLWKWKH
MCV 086.0 3.0 used lot.
THR 105 025 7KH¿UVWFROXPQFRQVLVWVRIWDUJHWYDOXHVDQGWKHVHFRQG
Hb 07.1 0.5 the allowable tolerance for the 6 measured parameters.
For example:
WBC: 2.4 m/mm3 ± 0.5 m/mm3
Reminder of selected Unit RBC: 2.48 M/mm3 ± 0.2 M/mm3
...
Hb: 7.1 g/dl ± 0.5 g/dl
+EYDOXHPXVWEHHQWHUHGDFFRUGLQJWRWKHXQLWVHOHFWHGIURP+E8QLW$UHPLQGHU
RIWKHVHOHFWHGXQLWLVGLVSOD\HGDERYHWKHWHVWVQDPH
DLB5
52
CONFIGURATION
4.4 SYSTEM MENU

2QWKHULJKWRIWKHWLWOHEDULVWKHYHUVLRQQXPEHURIWKH
SYSTEM n.nnxs program.
TIME
DATE To quit this sub-menu, press the Cancel key. Menus
COUNTRY are called in a hierarchical way. Each time you quit a sub-
COMMUNICATION PHQXWKHSUHYLRXVPHQXLVGLVSOD\HGXQWLO\RXUHDFKWKH
PRINTOUT home screen.
4.4.1 TIME
7KHFXUUHQWWLPHLVGLVSOD\HGGLYLGHGLQWRHGLW¿HOGV+RXU0LQXWH6HFRQG$0307KH
FKDQJHDEOH¿HOGLVLQLQYHUWHGFRORU7RPRYHIURPRQH¿HOGWRDQRWKHUXVHRight arrow or
Left arrow keys (and/or Tab or Shift-Tab keys on external keyboard).
7RFKDQJHD¿HOGYDOXHXVHUp arrow or Down arrow keys (or directly enter the numeric
YDOXHRQDQH[WHUQDONH\ERDUG
7KLVH[DPSOHLVJLYHQIRUDKPRGHFRQ¿JXUDWLRQ
SYSTEM TIME
V\VWHP:LWKDKPRGHFRQ¿JXUDWLRQV\VWHPWKH
Setup IRXUWK¿HOG$0RU30GLVDSSHDUV
7KLVODVW¿HOGFDQEHPRGL¿HGZLWKUp arrow
01:59:25 PM or Down arrow keys (or by entering A or P
characters on an external keyboard).
7RTXLWZLWKRXWYDOLGDWLQJWKHPRGL¿FDWLRQVXVH
the Cancel key.
7RTXLWDQGVDYHPRGL¿FDWLRQVXVHWKHEnter
key.
4.4.2 DATE
7KHFXUUHQWGDWHLVGLVSOD\HGDQGGLYLGHGLQWRHGLW¿HOGV0RQWK'D\<HDU7RPRGLI\WKH
GDWHSURFHHGDVH[SODLQHGDERYH
7KLVH[DPSOHLVJLYHQIRUDV\VWHPFRQ¿JXUDWLRQ
SYSTEM DATE
in the English language. For any other language,
Setup the display order Month and Day will be made
according to the country (Day, Month, Year in
French, for example).
02/15/2018
DLB5
53
CONFIGURATION
4.4.3 COUNTRY
6HOHFW WKH DFWLYH ¿HOG E\ PHDQV RI Left arrow or Right arrow keys (and/or Tab and
Shift+Tab on an external keyboard).
0RGLI\WKHVHOHFWHG¿HOGFRQWHQWE\PHDQVRIUp arrow or Down arrow keys.
COUNTRY:'H¿QHVXVHGODQJXDJH)UHQFK(QJOLVKDQG
LOCALIZATION a third customized language).
COUNTRY ENGLISH CLOCK: Choice between 12h or 24h format.
CLOCK 12 H KEYBOARD: External keyboard type used. Choice
between French (AZERTY), US (QWERTY) and third type.
KEYBOARD US NumLock: 'H¿QHV H[WHUQDO NH\ERDUG ORFNLQJ QXPHULF
NumLock ON keys, when power is turned on.
4.4.4 COMMUNICATION
1) Serial port #1: reserved use
BAUD RATE:'DWDÀRZRQ56SRUWWR

SERIAL PORT #1 Baud).
DATA: Data frame (7 or 8 bit).
BAUD RATE 19200 B PARITY:1RQH(YHQRU2GG
DATA 8 Bit Bio.Wi-Fi: 5HVHUYHGXVH
PARITY NONE Enter NH\ YDOLGDWHV GDWD FKDQJHV DQG SURYLGHV D
Bio.Wi-Fi second identical array for computer connection.
With an external keyboard, Page up and Page Down keys
allow running from one screen to another.
2) Serial port #2: Computer connection
Wi-Fi: 5HVHUYHGXVH
SERIAL PORT #2 Data Out: 7KLV DGGLWLRQDO RSWLRQ JLYHV WKH SRVVLELOLW\ WR
COMPUTER send results to a computer automatically at the end of the
analysis.
BAUD RATE 19200 B 3 options:
DATA 8 Bit - NONE: No automatic sending
PARITY NONE - Auto 1: Send results without histograms
Wi-Fi - Auto 2: Send results with histograms
Data Out NONE (excepted EO histogram in 5 part-diff mode)
See the ‘Export data’ chapter for more details on the sent
data structure.
)RUXVHRIWKLVRSWLRQWKHFRPSXWHUPXVWEHSHUPDQHQWO\DYDLODEOHWRUHFHLYHGDWD
DLB5
54
CONFIGURATION
4.4.5 PRINTOUT
This menu sets printout parameters.
PRINTOUT
Normal range Normal range:,IFKHFNHGYDOLGDWHV ) the normal

Histogram ; 1 range printing on the line following the test results line
on built-in printer. Normalities are always printed on an
EO Histogram
External Type 10 external printer.
1RWH7KH\ZLOORQO\EHSULQWHGLIWKH0D[LPXPYDOXHLVQRW
Auto Print ;
0 in the Min/Max arrays.
Header ;
Histogram:,IVHOHFWHGYDOLGDWHV ) the printing of the histograms on printers.

Internal printer: ; 1 and ;JLYHWKHVDPHUHVXOW
External printer: ; 1 prints fully black histograms, ; 2 just prints the histogram upper line in
order to reduce ink consumption on some printers.
EO Histogram:,IFKHFNHGYDOLGDWHV ) the printing of the EO histogram on the buit-in printer.

EO histogram is not printed on external printer.
External Type: External printer type used. Use or to select printer type.
1 for a graphic printer with ESC/P compatibility (e.g. EPSON® type)
2 for draft printing in character mode
3 for a graphic printer with PCL3 compatibility (e.g. Hewlett Packard® type).
(contact Dialab for the list of working printers or www.mslabos.com).
Auto Print:,IFKHFNHGYDOLGDWHV ) the automatic printing of analysis immediately after

counting, simultaneously when results are displayed.
Header: ;WRSULQWWKHSHUVRQDOL]HGKHDGLQJ2WKHUZLVHDYDOXHJLYHVWKHQXPEHURI

VNLSSHGOLQHVIURPWKHEHJLQQLQJRIWKHSULQWHGSDJHRQDQH[WHUQDOSULQWHU7KLVYDOXHLVLJQRUHG
on the built-in printer as the personalized heading is not printed.
PRINTING COMMANDS
Reminder: Printing can only be selected when the LED from the Print key is on.
Results:([FHSWIRUWKHFDVHRIDXWRPDWLFSULQWLQJSUHYLRXVO\GHVFULEHGUHVXOWVFDQ
be printed when one of the result screens is displayed.
Reports: A set of analysis results can be printed when the instrument displays the
home screen.
Calibration and Normalities: Calibration and normal range screens can be printed
only on the built-in printer when they are displayed
VHHSUHYLRXVO\µ&$/,%5$7,21¶FRHI¿FLHQWVSULQWRXWFKDSWHU
DLB5
55
CONFIGURATION
4.4.6 APPLICATION SUB-MENU

This command line calls a new sub-menu as shown below.
APPLICATION
To quit this sub-menu, press the Cancel key.
INFORMATION Menus are called in a hierarchical way. Each time you quit
DRIVERS DVXEPHQXWKHSUHYLRXVPHQXLVGLVSOD\HGXQWLO\RXUHDFK
HARDWARE the waiting screen.
UNITS
RESTORE BANKS
The HARDWARE and RESTORE BANKS line
appear only if the DLB5 is in administrator mode
(see section 6.4).
4.4.6.1 INFORMATION
VERSION:6RIWZDUHYHUVLRQQXPEHU
INFORMATION
DATE :/DVWVRIWZDUHPRGL¿FDWLRQGDWH
VERSION : 1.50Xs (YYYY/MM/DD format)
DATE : 2015-12-03 EXP. DAY: Number of remaining days before the expiration
EXP.DAY : 116 date of the reagent pack.
MSCARD : NEW MS-CARD : NEW: Appears only if a new MS-CARD is
inserted into the card reader.
Printout key can be used to print the program

YHUVLRQDQGWKH'/%VHULDOQXPEHUXVLQJWKH
built-in printer.
4.4.6.2 DRIVERS
DRIVERS
7KH ¿UVW VFUHHQ VKRZV WKH H[WHUQDO NH\ERDUG DQG 06
&$5'UHDGHUGULYHUYHUVLRQQXPEHUV7KHUHYLVLRQQXPEHU
DQGWKHGDWHRIHDFKGULYHUFRXOGFKDQJHDFFRUGLQJWRWKH
Keyboard decode SURJUDPYHUVLRQDGULYHUFDQEHDXWRPDWLFDOO\XSGDWHG
R1.07 19/08/09 if needed, with a program update).
MSCARD reader
R1.20s 12/01/10 Quit this screen by the Enter key to see the second
GULYHUVFUHHQCancel key FRPHVEDFNWRWKHSUHYLRXV
menu).
DRIVERS
7KLVODVWVFUHHQVKRZVWKHPRWRUGULYHUYHUVLRQQXPEHU
Syringe Motor
R1.00 12/06/09
Needle Motor Printout NH\FDQEHXVHGWRSULQWWKHGULYHUV
R1.00 04/02/09 YHUVLRQVDQGWKH'/%VHULDOQXPEHUXVLQJWKH
Carrier Motor built-in printer.
R1.01 30/10/09
DLB5
56
CONFIGURATION
4.4.6.3 HARDWARE
)URPWKHYHUVLRQWKH+$5':$5(VXEPHQXLV
accessible only if the DLB5 is in administrator mode
(see 6.4 section).
The screen below, which includes the vacuum parameters, must be changed
with great care and if possible with the help of a Dialab technician. If
incorrect data is entered then the analysis results will be inaccurate.
VACUUM #1:6HWVWKHFRXQWLQJYDFXXPRIWKH5%&7+5
HARDWARE and WBC canals in mBars.
VACUUM EO:6HWVWKHFRXQWLQJYDFXXPRIWKH(2FDQDO
VACUUM #1 290
VACUUM EO 290
in mBars.
CAP PIERCING CAP PIERCING:2SWLRQQRW\HWDYDLODEOH
NEEDLE BOTTOM 790 NEEDLE BOTTOM: Indicates the sample needle bottom
SRVLWLRQYDOXHLQWRWKHEORRGVDPSOHWXEH0RGLI\WKLVYDOXH
if the needle contacts the tube bottom assuming it is not
of standard shape. 1 mm corresponds to approx.10 units
of this parameter.
4.4.6.4 UNITS
UNIT SYSTEM
Hb Unit: Selects the unit in which Hb, MCHC and MCH or
+FWUHVXOWVDUHJLYHQ
- Hb Unit : Std Std: Hb and MCHC in g/dl, MCH in pg
- SI: Hb and MCHC in mmol/l, MCH in fmol
- IU: Hb and MCHC in g/l, Hct 0. XX without unit
Note: 5HVXOWV$1' 1RUPDO UDQJH IRU KDHPDWRORJ\ DUH JLYHQ LQ WKH VHOHFWHG XQLWV 1RUPDO
5DQJHYDOXHVIURP3DUDPHWHUVVFUHHQPXVWDOVREHHQWHUHGDFFRUGLQJWRWKLVXQLWIRUWKHVH
parameters.
DLB5
57
CONFIGURATION
4.4.6.5 RESTORE BANKS
The RESTORE BANKS sub-menu is accessible only if

the DLB5 is in administrator mode (see 6.4 section).
This screen allows the operator to restore bank parameters to their factory setup (reset
parameters).
FACTORY SETUP HUMAN BANKS: Male, Female and New-born banks

restore.
HUMAN BANKS VETERINARY BANKS: Not used.
VETERINARY BANKS FREE BANKS: Free #1 and Free #2 banks restore.
FREE BANKS CONTROL BANK: Control bank restore.
CONTROL BANK Quit this screen by pressing the Cancel key to come
back to the application sub-menu.
BANKS
HUMAN BANKS Press the Return key to reset the selected banks (e.g.
RESETED TO HUMAN BANKS).
DEFAULT VALUES Then strike any key to go back to the factory setup screen.
Strike a key...
DLB5
58
CONFIGURATION
4.5 PRIME/RINSE MENU

PRIME/RINSE 7KH WHFKQLFDO PHQX DOORZV VSHFL¿F LQWHUYHQWLRQ RQ WKH
ÀXLGLFF\FOHVRIWKHLQVWUXPHQW
PRIMING REAGENTS ,W PXVW EH XVHG RQO\ LQ VSHFL¿F FDVHV DSDUW IURP WKH
PRIMING RINSE SOL. standard analysis cycles.
DRAINING
Hb REFERENCE
UNBLOCK APERTURE
H²O RINSING
4.5.1 PRIMING REAGENTS

Primes all the reagents except the rinsing solution. This cycle must be carried out when
the reagentORWV DUH changed.
4.5.2 PRIMING RINSE SOL.

Primes only the rinsing solution. This cycle must be carried out when the reagent pack is changed.
4.5.3 DRAINING
Drains the measuring and dilution chambers. It must be used in case of failure of the standard
liquid draining procedure caused by a functional anomaly.
4.5.4 Hb REFERENCE
Performs a cycle with a short simulated counting time.
7KLVF\FOHLVXVHGWRFRQWUROWKHKHPRJORELQUHIHUHQFHDQGHYHQWXDOO\FRUUHFWWKHSKRWRPHWHU
automatic calibration.
This cycle is automatically called after a startup or a standby and after priming reagents at
power up.
NEVER RUN THIS CYCLE WITH THE

INSTRUMENT FRONT COVER OPEN.
4.5.5 UNBLOCK APERTURE

7REHFDUULHGRXWLIVHYHUDOFKDQQHOVDUHVLPXOWDQHRXVO\DQGIUHTXHQWO\EORFNHGZKLOHDQDO\VLV
After this procedure, run an analysis without blood to rinse chambers.
DLB5
59
CONFIGURATION
4.5.6 H²O RINSING

7KHDLPRIWKLVSURFHGXUHLVWRULQVHDOOWKHÀXLGLFFLUFXLWVZLWKGLVWLOOHGZDWHUWRHOLPLQDWHWKH
UHDJHQWVZKLFKFRXOGEHFRPHFU\VWDOOL]HGLQWKHWXEHVDQGYDOYHV
This procedure must be carried out when the instrument will not be used for a long time (more
than a week) or before transportation.
Selecting this menu item displays the screen below to inform the operator of the necessary
handling required to implement this operation.
Operating mode
1) Use a container of distilled water (Rinsing solution can also be used).

2) Take the cap off the Rinse Solution and insert the tube into the distilled water container or
XQVFUHZWKHFDSRIIWKH5LQVH6ROXWLRQFRQWDLQHUZKLOHOHDYLQJWKHWXEHLQVLGH
3) Take off the cap of the DILUENT container, take the tube
H²O RINSING out and let it drain as much as possible. Insert the tube
into the distilled water container.
Put all reagent tubes
4) Repeat the same procedure with the DETERGENT,
in DISTILLED water
then
LYSE and EO LYSE tubes.
Note: Make sure that there is enough distilled water in the
container so that all the tubes are correctly plunged into
Validate... the water.
6HOHFW WKH 5,16,1* +ð2 F\FOH WR GLVSOD\ WKH DERYH
VFUHHQDQGYDOLGDWH
7KHLQVWUXPHQWZLOOFDUU\RXWVHYHUDOULQVLQJF\FOHVDQGUHMHFWDOOWKHUHDJHQWVIURPWKHWXEHV
into the waste container.
6) Unplug all tubes from the instrument and empty them as much as possible.
3XWWKHSURWHFWFDSVEDFNRQWKHÀXLGLFFRQQHFWRU
DLB5
60
CONFIGURATION
4.6 STANDBY MENU

$VWDQGE\SURFHGXUHFDQEHSHUIRUPHGRQWKHLQVWUXPHQWWRJXDUDQWHHWKDWWKHÀXLGLFFLUFXLWV
stay well-rinsed to reduce the crystallization risks due to drying reagents . This can occur if the
instrument is not going to be used for a long time.
,WLVQRWDQHQHUJ\VDYLQJVWDWH
+RZHYHUWKHUHLVQRGLVDGYDQWDJHLQVZLWFKLQJRIIWKHLQVWUXPHQWZKHQWKHVWDQGE\F\FOHLV
¿QLVKHG
,WLVHYHQUHFRPPHQGHGWRWXUQRIIWKHDQDO\]HUUHJXODUO\RQFHDZHHNIRUH[DPSOH
The STANDBY menu is displayed as below.
The instrument can be turned to standby mode automatically or manually.
EVENING: Automatic standby at a set hour.

STANDBY Select NONE and, by Up arrow or Down arrow keys,
FKRRVHWKHGHVLUHGKRXUPRGL¿FDWLRQE\PQVWHSV
EVENING NONE AUTO: Automatic standby for a programmed period after
AUTO NONE an analysis.
NOW Select NONE and, by Up arrow or Down arrow keys,
FKRRVHWKHGHVLUHGWLPHPRGL¿FDWLRQE\PQVWHSVLQD
range of 30 mn to 7 h 30 mn). The instrument will be turned
to standby mode according to the programmed time from
this moment, the time will be reinstated after each new test.
It is necessary to use the Enter NH\ WR VDYH D PRGL¿HG GDWD LQ ERWK RI
the above cases. If you quit the program by using the Left arrow key, the
PRGL¿FDWLRQVZLOOQRWEHVDYHG
,IERWKFRQ¿JXUDWLRQVDUHSURJUDPPHGWKH¿UVWWLPHUHDFKHGZLOOVWDUWWKHVWDQGE\PRGH
NOW: The instrument starts immediately the rinsing cycle with the rinsing solution before turning
to standby.
When the instrument is in standby mode, the display is off and the white LED (power indicator)
blinks.
IMPORTANT
Never leave the instrument more than one day without performing an analysis
or setting the standby mode. The correct method is to implement the standby
procedure in the evening or before turning off the power after the last analysis
of the day.
DLB5
61
CONFIGURATION
When the instrument displays the home screen, it beeps

Sq 0101 Bk Free #1 each minute to point out the remaining time before automatic
standby.
AUTOMATIC
STANDBY IN 9mn
March Mon 19, 2018

12h 59mn AM
Return to normal state
1) The appliance has been switched off after the standby procedure:
:KHQWKHSRZHULVWXUQHGRQWKHGHYLFHZLOOGRWKHIROORZLQJ
a) Rinsing liquid disposal.
b) Reagents priming.
2) The appliance has not been switched off after a standby procedure:
6LPSO\ WRXFK DQ\ NH\ WR UHDFWLYDWH WKH GLVSOD\7KH ZRUG 5HDG\ written in green has been
replaced by 6WDQGE\ written in blue.
All manipulations, such as setting parameters, recalling analysis, printing... can be carried out
without quitting the standby mode.
7KHGHYLFHWXUQVEDFNDXWRPDWLFDOO\WRVWDQGE\PRGHDIWHUDVKRUWWLPHRQFHLWKDVUHWXUQHGWR
the home screen.
The only way to quit the standby mode is to run a new analysis. The appliance then carries out
the rinsing cycles as described in 1) before asking for the sampling blood tube.
Standby cycles followed by wake-up cycles consume reagents. Do not switch

WKHGHYLFHWRVWDQGE\PRGHPRUHWKDQRQFHDGD\LWLVQRWQHFHVVDU\DQG
ZRXOGUHGXFHWKHQXPEHURIDYDLODEOHDQDO\VHVFRQWDLQHGLQWKHUHDJHQWSDFN
DLB5
62
CONFIGURATION
4.7 CLEAR ANALYSES MENU

Selection of this item in the main menu displays the screen below.
If selected by mistake, press the Escape Key to exit

CLEAR ANALYSES this screen.
ATTENTION
Clear ALL analyses ,IWKLVRSWLRQLVYDOLGDWHG ), the following occurs:
- in memory All ‘Patient’ results are cleared from memory.
- All ‘Quality Control’ results are cleared from memory.
Cancel
- 7KH¿OH,'DQGWKHVHTXHQFHQXPEHUVDUHUHVHWHGWR1.
&RQ¿UP
WARNING
:KHQFRQ¿UPHGWKHPHVVDJHEHORZLVGLVSOD\HG
and results are irreversibly erased.
CLEAR ANALYSES
ALL ANALYSES
CLEARED !
Strike a key...
DLB5
63
CONFIGURATION
DLB5
64
5: QUALITY
CONTROL
DLB5
65
QUALITY CONTROL
5.0 USE OF BIOLOGICAL CONTROLS

Performing biological quality controls is a well established method of controlling
performance hematology instruments. The use of stabilized blood cell preparations
DOORZV PRQLWRULQJ RI VWDELOLW\ H[DFWQHVV DQG DFFXUDF\ RI PHDVXUHG YDOXHV RYHU D ORQJ
SHULRG DV ZHOO DV HDUO\ GHWHFWLRQ DQG SUHYHQWLRQ RI SUREOHPV
,W LV DGYLVDEOH WR FDUU\ RXW D TXDOLW\ FRQWURO
• before any start of operation,
• after maintenance,
• in case of doubt about the accuracy of the analysis results.
:HUHFRPPHQG5 '&%&'ZKLFKLVDWULOHYHOKDHPDWRORJ\FRQWUROGHVLJQHGWR
GRFXPHQWDQGPRQLWRUYDOXHVREWDLQHGIURPKDHPDWRORJ\FHOOFRXQWHUVWKDWXVHWKH
impedance counting principle.
R&D CBC-3D® contains human erythrocytes, simulated leukocytes, and mammalian

SODWHOHWVLQDSODVPDOLNHÀXLG,WLVRQO\IRULQYLWURGLDJQRVWLFXVH
This preparation must be handled carefully as if it was a patient sample.
%LRORJLFDO ÀXLGV DUH SRWHQWLDOO\ KD]DUGRXV ELRORJLFDO PDWHULDOV

Human serum was used in the manufacture of this product.
This material should be handled as if capable of transmitting infec-
tious disease.
Take appropriate precautions when handling all laboratory samples to
prevent skin contact (wearing of gloves), ingestion, or inhalation.
Stability and storage

Do not freeze. Store between 2°... 8°C when not in use. Unopened tubes are stable
XQWLO WKH H[SLUDWLRQ GDWH RQ WKH ODEHO 2SHQHG WXEHV DUH VWDEOH IRU GD\V DIWHU ¿UVW
XVH SURYLGHG WKDW WKH\ DUH KDQGOHG SURSHUO\
5HIHUWRWKHXVHU¶VJXLGHJLYHQZLWKWKHNLWRUZLWKWKHWXEHIRUPRUHLQIRUPDWLRQ
(See next page for references)
DLB5
67
QUALITY CONTROL
',$/$%UHFRPPHQGVWKHXVHRIFRQWUROEORRGIURPWKHFRPSDQ\5 'SOHDVHFRQWDFW
WKHLUORFDOVXSSOLHULQ\RXUDUHD
DLB5
68
QUALITY CONTROL
5.1 RUN QUALITY CONTROL ANALYSIS

RUN QUALITY CONTROL ANALYSIS
Quality control allows tests with control blood to check the correct calibration of the appliance
DWUHJXODULQWHUYDOV
It is performed as a standard blood analysis from the QUALITY CT menu item.
:KHQ WKLV LWHP LV VHOHFWHG WKH FRQWURO EORRG OHYHO WR
3 PART ANALYSIS DQDO\]HLVGLVSOD\HGRQWKHULJKW7RFKDQJHWKHOHYHOVHH
Sq : 0101 the parameters menu chapter.
7KHFRQWUROEORRGPXVWEHLGHQWL¿HGWRDXWKRUL]HWKHVWDUW
of the test.
ANALYSIS
QUALITY CT LOW
The control blood must be identified to
authorize the start of the test.
(See section 4.3.5)
7KHVFUHHQZKLFKLGHQWL¿HVWKHRSHUDWRULVGLVSOD\HGDWWKHVWDUWRID4XDOLW\&RQWUROWHVW
Reminder of the control blood level

CONTROL LOW
LOT EXP.DAY 5HPLQGHURIWKHFRQWUROEORRGLGHQWL¿FDWLRQ

123.45 01/02/2018
OPERATOR
GF 2SHUDWRULGHQWL¿FDWLRQ
Initials only on two letters
COMMENT
Optional comments
Easier with an external alphanumeric keyboard
$¿UVWKLWRQWKHEnter NH\PRYHVWKHFXUVRUWRWKHVHFRQGOLQHWRHGLWFRPPHQWVDQGDVHFRQG
KLWRQWKLVVDPHNH\DFWLYDWHVWKHWHVW:LWKDQH[WHUQDONH\ERDUGWKHPage upNH\PRYHVWKH
HGLWFXUVRUEDFNWRWKHSUHYLRXVOLQH
Differences between a standard analysis and a Quality Control analysis

:KHQWKHWHVWLV¿QLVKHGWKHUHVXOWVDUHGLVSOD\HGDVDVWDQGDUGEORRGDQDO\VLV
EXWWKH0LQPD[DODUPVDUHRQO\PDQDJHGE\WKHSDUDPHWHUVGH¿QHGIRUWKHFRQWUROEORRG
)URPWKHYHUVLRQWKHDODUP5LVDGGHGWRWKHWLFNHWD4&DQDO\VLV4XDOLW\&RQWUROLQDGGLWLRQ
to the up / down arrows in case of threshold alarm. Only the 3 main parameters WBC, RBC
and PLT are managed, the R appearing on all the results associated with the main parameter:
under GB populations, parameters associated with RBC (MCV, Hct, TCMH, MCHC, RDW and
Hgb) and / or PLT (MPV, Pct and IDP).
Only the 5 parameters normalities are printed.

The barcode reader is not accepted for quality control.
DLB5
69
QUALITY CONTROL
Recording
7KHUHVXOWVDUHVDYHGLQFRPSDFWIRUPLQDVHSDUDWHDUUD\IRUEORRGDQDO\VHV8SWRRU
TXDOLW\FRQWUROEORRGDQDO\VHVFDQEHVDYHG
7KHVHDQDO\VHVKDYHDGLIIHUHQWVHTXHQFHQXPEHUIURPWKHEORRGDQDO\VLV
* DLB5 manages up to 125 Q.C. analyses, only if it is

HTXLSSHGZLWKDPRWKHUERDUGUHYRUKLJKHU
5.2 RECALL ANALYSIS

Select the ANALYSES item in the main menu to recall
CONSULTATION
analyses.
ANALYSES
:KHQ TXDOLW\ FRQWURO DQDO\VHV KDYH EHHQ VDYHG DQ
QUALITY CTRL intermediate menu allows the choice of analysis type to
recall.
The ‘SELECT ANALYSIS’ menu for quality control is

SELECT ANALYSIS different from the blood analysis menu as follows:
- 0006 02/03 2:53PM Nl - The selected number is the sequence number and not
0005 02/03 2:50PM Lw WKH¿OHQXPEHUZKLFKGRHVQRWH[LVWLQWKHVHWHVWV
0004 02/03 2:47PM Hi %ORRGDEEUHYLDWLRQVDUHGLIIHUHQW
0003 02/02 7:51AM Lw /Z/RZOHYHOFRQWUROEORRG
0002 02/02 7:48AM Nl 1O1RUPDOOHYHOFRQWUROEORRG
0001 02/02 7:45AM Hi +L+LJKOHYHOFRQWUROEORRG
The recall of a quality control analysis is only displayed on two screens:

7KH ¿UVW VFUHHQ VKRZV WKH PHDVXUHG SDUDPHWHUV UHVXOWV WKH VHFRQG VKRZV WKH DQDO\VLV
LGHQWL¿FDWLRQZKLFKFDQEHPRGL¿HGZLWKDQDOSKDQXPHULFH[WHUQDONH\ERDUGE\SUHVVLQJWKH
F4 function key or with the Up Arrow key of the internal keyboard.
7KHRWKHUYDOXHVDQGKLVWRJUDPVDUHQRWVDYHGLQTXDOLW\FRQWUROPRGH
The results will also be printed in reduced form. Full printing is only possible immediately after
the test.
DLB5
70
PRINTING REPORTS
5.3 REPORTS
It is possible to print a set of analysis results in list or graphic type.
Operating mode
When the appliance is on home screen, press the Print key or Print screen key on an external
NH\ERDUGWKLVZLOORQO\EHDFWLYDWHGLIDWOHDVW21(DQDO\VLVH[LVWVLQWKHGHYLFHPHPRU\
,IERWKEORRGDQGTXDOLW\FRQWURODQDO\VLVH[LVWD¿UVWVFUHHQVKRZQEHORZLVGLVSOD\HGDOORZLQJ
to choose the analysis type to report:
PRINTOUT Select ANALYSES to print out the analyses statistics report

(C.V.) to internal or external printer.
ANALYSES
Q.C. LISTING
L.J. GRAPH
PRINTOUT Select Q.C LISTING to print out a quality control report

(listing type) to internal or external printer.
ANALYSES
Q.C. LISTING
L.J. GRAPH
PRINTOUT
Select L.J. GRAPH to print out a quality control report
ANALYSES /HYH\-HQQLQJVJUDSKW\SHWRLQWHUQDOSULQWHUH[WHUQDO
Q.C. LISTING printer is not managed). You must select the parameter
L.J. GRAPH WBC which will be printed out: WBC, RBC, MCV, THR or Hb.
DLB5
71
PRINTING REPORTS
,IRQO\DQDO\VLVLVVWRUHGLQWKHGHYLFHPHPRU\WKHSULQWLQJVWDUWVLPPHGLDWHO\,IQRWDVHOHFWLRQ
is proposed.
The screen on the left is displayed in two steps.

STATISTICS
Print 21 analyses 7KH¿UVWOLQHGH¿QHVWKHQXPEHURIDQDO\VHVWRSULQW
- Up to File : If there are less than 21 analyses in memory, the

number suggested by default will be the number of
00215-006 02/15 12:59P Ct analyses in memory.
- If there are more than 21 analyses in memory, the
number suggested by default will be 21.
&KDQJHWKHYDOXHE\WKHUp arrow or Down arrow keys. Validate ( ) when the displayed

YDOXHLVFRUUHFW1RPRUHWKDQWHVWVFDQEHSULQWHGDWRQFH
2) If the number of analyses to print is equal to the quantity of analyses in memory, printing
starts immediately.
,IQRWWKHVHFRQGOLQHLVGLVSOD\HGWRVHOHFWWKHODVWDQDO\VLV¿OHQXPEHU7KHQXPEHUVXJJHVWHG
E\GHIDXOWLVWKH¿OHQXPEHURIWKHODVWDQDO\VLVVWRUHG
&KDQJHWKH¿OHQXPEHUE\WKHUp arrow or Down arrow keys.
Start the printing with Enter key.
The list of the printed analyses will always begin with the most recent analysis.
5.3.1 Blood analysis report

$OOWKHUHVXOWVDUHSULQWHGLQOLVWIRUPDW$IWHUWKHVHUHVXOWVDOLQHJLYHVWKHPD[LPXPJDSEHWZHHQ
HDFKWHVWH[DFWYDOXHQRWDSSUR[LPDWHDQGDQRWKHUOLQHJLYHVWKHYDULDWLRQFRHI¿FLHQWRIHDFK
test. Both lines will only be printed if at least 2 results are selected.
Therefore both lines only mean something if the list of printed results concerns the same blood.
On the built-in printer, only the main tests are printed because of the small width of the paper.
6RPHYDOXHVDUHURXQGHGIRUWKHVDPHUHDVRQV
5.3.2 Quality control report

5.3.2.1 Q. C. Listing
Results are grouped together according to the control blood lot numbers.
2QWKHEXLOWLQSULQWHU¿JXUH
$¿UVWOLQHVKRZVWKHFRQWUROEORRGWDUJHWYDOXHVDQGWKHSHUFHQWDJHJDSFRPSDUHGZLWKWKH
WDUJHWYDOXHIRUHDFKDQDO\VLV
- On an external printer
7KHUHVXOWYDOXHLVIROORZHGE\WKHSHUFHQWDJHJDSFRPSDUHGZLWKWKHWDUJHWYDOXH,IPRUH
WKDQRQHDQDO\VLVLVSULQWHGIRUWKHVDPHORWQXPEHUWKHJDSEHWZHHQWKHWZRH[WUHPHYDOXHV
is printed on an extra line.
In both cases, if a gap is higher than 99%, it is shown by two stars.
DLB5
72
PRINTING REPORTS
Figure 5.3.2.1
5.3.2.2 L.J. Graph

/HYH\-HQQLQJVW\SHJUDSK
2QO\¿YHSDUDPHWHUVDUHPDQDJHG:%&5%&7+50&9DQG+E
The external printer is not managed.
5HVXOWVDUHJURXSHGWRJHWKHUDFFRUGLQJWRWKHFRQWUROEORRGORWQXPEHUVDQGOHYHOVHHIROORZLQJ
examples (Figures 5.3.2.2a to 5.3.2.2e).
If a result is out of range (lower than -2D or higher than +2D) it is marked R (Rejected).
Figure 5.3.2.2a Figure 5.3.2.2b Figure 5.3.2.2c
Figure 5.3.2.2d Figure 5.3.2.2e
Date of the last analysis
'DWHRIWKH¿UVWDQDO\VLV
DLB5
73
DLB5
74
6: MS-CARDS
DLB5
75
MS-CARDS
6.1 REAGENT MS-CARD

7KHGHYLFHFDQZRUNZLWKRXWDQ06&$5'
IRU PDQDJHPHQW DFFHVV FRQ¿JXUDWLRQ
analysis recall...) but a card is absolutely
necessary to carry out an analysis. It
HQDEOHV WR FRQWURO WKH YDOLGLW\ RI WKH
reagents and to manage their expiry date.
Hologram
IMPORTANT
Check that the card outside appearance is conformed:
- That there is the genuine hologram.
- That there are all the written legal notices of property:
“Property of BV Rights - Vaduz - FL”.
Please read the License agreement at the end of the manual.
$06&$5'LVSURYLGHGZLWKHDFK'LDGLIIUHDJHQWDQGDVSDUH06&$5'LVSURYLGHG
ZLWKWKHGHYLFHZKHQLWOHDYHVWKHIDFWRU\
At each change ofDVHWRI reagentV,

insert the new card enclosed with it.
Use-by-date of reagents
7KHGDWHRQWKH/<6(UHDJHQWJLYHVWKHOLPLWRIYDOLGLW\RIXQRSHQHGSURGXFWV
When the bottles are open, their use is limited to 4 months, controlled by the MS-CARD which
must be inserted into the instrument at each change of DVHWRIUHDJHQWV. If used
beyond, the analysis results will be inaccurate.
The number of days remaining before the expiry date can be controlled from the menu SYSTEM/
APPLICATION/INFORMATIONS.
When the expiry date is reached, a message is displayed at the power up of the instrument or
ZKHQDQDQDO\VLVLVUXQDIWHUDVWDQGE\7KHGHYLFHDXWKRUL]HVDQDO\VHVXSXQWLOGD\VDIWHU
the expiry date, beyond, a new MS-CARD is required.
Note:7KHGHYLFHFDQGLVSOD\WKHH[SLU\GDWHGHDGOLQHPHVVDJHHYHQZLWKDQHZFDUGLQWURGXFHG
into the card reader. It is necessary to run an analysis for recording the new expiry date in the
memory.
Control of the analyses number
The MS-CARD also controls the maximum number of authorized analyses by reagent VHW. At
the end of an analysis, a message is displayed when there are 20, then 10 analyses left
and when the last analysis has been done.
+RZHYHUDFFRUGLQJWRWKHIUHTXHQF\RIXVHRIWKHLQVWUXPHQWRQHUHDJHQWIURPWKHSDFNPD\
be empty before you reach the maximum number of authorized analyses.
DLB5
77
MS-CARDS
6.1.1 Blood banks
7KH06&$5'FRQWUROVWKHFKRLFHRQWKHDSSOLFDWLRQDQGJLYHVDFFHVVWRWKHFRUUHFWEORRGEDQNV
Below are the different blood banks associated with both applications.
%ORRG%DQN 1E $EY
Male 1 Hm
Female 2 Fm
New born 3 Nn
Control 4 Ct
Free #1 5 L1
Free #2 6 L2
2ULJLQDOSDUDPHWHUVRIEDQNV)UHHDQG)UHHDUHQRWGH¿QHG
They are intended for loading card parameters.
DLB5
78
MS-CARDS
6.2 BLOOD BANK MS-CARD

NOT AVAILABLE.
DLB5
79
MS-CARDS
6.3 CONTROL BLOOD MS-CARD

7KHFRQWUROEORRG06&DUGSHUPLWVWRUHFRUGDOOFRQWUROEORRGSDUDPHWHUVRQWKUHHOHYHOVWR
perform quality control tests. See section 4.3.5 to get more information about these parameters.
,QVHUWWKHFDUGRQO\ZKHQWKHGHYLFHVKRZVWKHKRPHVFUHHQWKHQWKHVFUHHQEHORZDSSHDUV
The lot number and the expiry date are displayed.
CONTROL BLOOD
102CONTROL-DIFF
Lot: B110
Exp. date: 02-05-2018
Record This choice copies the control blood data into

Cancel the instrument.
This choice cancel the operation.
Operating mode
Verify the Lot number and the expiration date, then press the Enter key to record the new
parameters into the instrument. Be careful, old data are automatically replaced by the new ones.
$WWHQWLRQ7KHOHWWHULQIURQWRIWKHORWQXPEHULVQHYHUUHFRUGHG
CONTROL LOW
7R YHULI\ WKH QHZ SDUDPHWHUV XVH 3$5$0(7(56

LOT NUMBER
110 00
48$/,7<&7DQGYHULI\WKHORWQXPEHUDQGWKHH[SLU\GDWH
EXPIRY DATE (See section 4.3.5)
02 05 2018
If the expiry date is out of date the screen below is displayed.
CONTROL BLOOD
102CONTROL-DIFF
MS-Card with
lot out of date
Remove the MS-CARD
DLB5
80
MS-CARDS
6.4 ADMIN MS-CARD
The DLB5 works by default in user mode.

In order to change to administrator mode, when the home screen is displayed, insert the ADMIN
MS-Card.
Only one ADMIN MS-Card is supplied with the

analyser. It must be kept preciously during all
the instrument life by the responsible of the
laboratory.
In the administrator mode, the PARAMETERS menu is accessible (see section 4.3) as well as the
HARDWARE sub-menu (see section 4.4.6.3). This card does not permit to launch an analysis.
Once the ADMIN card is inserted, the PARAMETERS line is added to the main menu ( ).
User mode Administrator mode
Main menu Main menu
MANAGEMENT MANAGEMENT
RECALL ANALYSIS RECALL ANALYSIS
SYSTEM PARAMETERS
TECHNIC SYSTEM
STANDBY TECHNIC
CLEAR ANALYSIS STANDBY
CLEAR ANALYSIS
Also, when the ADMIN card is inserted, the HARDWARE line is added to the application sub-menu.
User mode Administrator mode

Application menu Application menu
APPLICATION APPLICATION
INFORMATIONS INFORMATIONS
DRIVERS DRIVERS
UNITS HARDWARE
RESTORE BANKS UNITS
RESTORE BANKS
Finally, the ADMIN card is necessary with the AUTO CALIBRATION (see 7.2 section).
DLB5
81
MS-CARDS
DLB5
82
7: CALIBRATION
DLB5
83
CALIBRATION
7.1 BANK CALIBRATION

NOT AVAILABLE
DLB5
85
CALIBRATION
7.2 AUTO CALIBRATION

The operating mode described hereafter can be used to calculate automatically the DLB5
FDOLEUDWLRQFRHI¿FLHQWVIRU7+55%&:%&+EDQG0&9039LVQRWPDQDJHG
)LUVWWKHWDUJHWYDOXHVIRUWKHOHYHO1250$/RIDFRQWUROEORRGPXVWEHYDOLGVHHKRZWRHQWHU
ORWQXPEHUDQGWDUJHWYDOXHVZLWKVHFWLRQ
1) Run a NORMAL control blood analysis with bank CONTROL selected.

2) Quit the results screen to return to the home screen.
3) Press the start analysis key to run a new analysis.
If the analysis has no error and
If WBC result is inside the range 4,00 - 12,00 m/mm3,
If THR result is inside the range 120 - 370 m/mm3,
the analysis screen includes an additional line : AUTO CALIBRATION.
3 PART ANALYSIS
Sq : 0127
ANALYSIS CONTROL
QUALITY CT
RECALL
AUTO CALIBRATION
D6HOHFWWKH$872&$/,%5$7,21OLQHDQGYDOLGDWHZLWKWKHenter key.
7KHEHORZVFUHHQLVGLVSOD\HGLIWKH'/%YHUVLRQLVRUPRUH
If not, the screen describes at the 4b) paragraph is directly displayed.
Insert an ADMIN MS-Card in order to go through the next stage (see 6.4 section).
It is possible to cancel the operation with the key and the DLB5 returns to the home page.
CALIBRATION
Insert ADMIN MS-Card
Cancel
DLB5
86
CALIBRATION
E7KHROGDQGQHZFRHI¿FLHQWVDUHGLVSOD\HG&KHFNRUXQFKHFNWKHFKHFNER[ZLWKWKHstart
analysis NH\)RUVSDFHEDUZLWKH[WHUQDONH\ERDUGWRDFFHSWRUQRWWKHQHZFRHI¿FLHQW
Then press the enter NH\ WR VDYH WKH QHZ FKHFNHG
CALIBRATION
FRHI¿FLHQWV
OLD NEW The DLB5 goes back to the home screen.
Coef THR 0.82 0.89 Then press again start analysis key to display the
Coef RBC 0.96 0.97 DQDO\VLV PHQX DQG YHULI\ WKH FRUUHFWHG UHVXOWV ZLWK WKH
Coef WBC 0.99 1.01
Coef Hb 1.10 1.11 item RECALL.
Coef MCV 0.97 0.96
1RWH&RUUHFWHGUHVXOWVDUHQRWVDYHGLQWKHPHPRU\7KHVDYHGUHVXOWVPXVWEHGLVFDUGHG
EHFDXVHWKH\DUHLQYDOLG
Note 2: Do not recall the analysis by the RECALL ANALYSIS menu because, in this case,
the results will be those of the original analysis.
WARNING:
If the difference between the old and the new

FRHI¿FLHQWLVKLJKHUWKDQQRFRUUHFWLRQLVGRQH
and so New coef = Old coef.
The automatic calibration does not applied in three cases:
D,IWKHGLIIHUHQFHEHWZHHQWKHROGDQGWKHQHZFRHI¿FLHQWLVKLJKHUWKDQ
E,IWKHQHZFRHI¿FLHQWLVKLJKHURUHTXDOWR
F,IWKHQHZFRHI¿FLHQWLVORZHUWKDQ
If one (or more) of those three cases is true,

the parameter line is written in red and the checkbox is replaced with a red-cross box,
DQGWKHFRHI¿FLHQWZLOOQRWEHFKDQJHG
CALIBRATION
OLD NEW
Coef THR 0.82 0.89
Coef RBC 0.96 0.97
Coef WBC 0.99 0.99
Coef Hb 1.10 1.11
Coef MCV 0.97 0.97
DLB5
87
CALIBRATION
7.3 MANUAL CALIBRATION

7KH'/%HTXLSHGZLWKDQDQWHULRUYHUVLRQWKDQWKHYHUVLRQGRHVQRWKDYHDQDFFHVVWR
the automatic calibration. The calibration will be done manually.
1) Run a sample of normal control blood using control bank and consult the results making
sure that there are no clogged alarm and homogeneity error.
Note the tests which need a correction or print them.
2) Quit the result screen to come back to the home page.
3) Display the calibration parameters (MENU/PARAMETERS/CALIBRATION).
)URPWKHYHUVLRQWKHDFFHVVRIWKLVPHQXUHTXLUHVWKHLQVHUWLRQRI
an MS-CARD ADMIN on the DLB5 (see section 4.1 and 6.4).
0RGLI\WKHFRUUHFWLRQFRHI¿FLHQWDFFRUGLQJWRWKHWDUJHWYDOXH9DOLGDWHSDVVLQJWKURXJKWKHQH[W
screen and go back to the home page.
4) Press the key to launch a new analysis. In the screen,

ANALYSIS 3 POP DVXSSOHPHQWDU\5(&$//OLQHDSSHDUVZKHQWKHSUHYLRXV
analysis is already in memory.
Sq : 0127
0RYHWKHVHOHFWLRQRQWKH5(&$//OLQHDQGYDOLGDWH7KLV
allows to display the corrected results according to the new
ANALYSIS : Control FDOLEUDWLRQSDUDPHWHUVDQGWRFRQWUROLIWKHPRGL¿FDWLRQV
CT QUALITY are correct.
RECALL If not, repeat the operation since step 2).
1RWHWKHFRUUHFWHGUHVXOWVZLOOQRWEHUHFRUGHGGRQ¶WWDNHWKHVDYHUHVXOWVLQWRDFFRXQW
Note 2 : recall the analysis on this way to control the impact of the calibration correction.
Do not recall the analysis by RECALL ANALYSIS menu because the results will be those recorded
during the original analysis.
IMPORTANT
0RGLI\ WKH IDFWRU\ FDOLEUDWLRQ FRHI¿FLHQWV JHQHUDO FRHI¿FLHQWV

only for a recalibration with control bloods, on the Control bank.
DLB5
88
8: MAINTENANCE
DLB5
89
MAINTENANCE
8.0 SAFETY
Personnel carrying out the DLB5 maintenance must be informed of the dangers linked
to the analyzer.
- ELECTRICAL HAZARD.
- SHARP OR ANGULAR COMPONENTS HAZARD.
- BIOLOGICAL RISK
ELECTRICAL HAZARD
Switch the analyzer off and unplug the power cord from
its socket before any maintenance is carried out inside the
DLB5 .
SHARP OR ANGULAR
COMPONENTS HAZARD
Some components inside the DLB5 have sharp edges or
angular corners.
Operate with caution to avoid cuts to the hands.
BIOLOGICAL RISK
When you carry out DLB5 maintenance, take
the same precautions as the one you take for handling
biological samples and wear protective garments, gloves
and glasses.
:DVWH ÀXLGV UHDJHQWV DQG LQIHFWLRXV ZDVWHV PXVW EH
disposed in accordance with local/national regulations.
DLB5
91
MAINTENANCE
DLB5
92
MAINTENANCE
8.1 INSTRUCTIONS FOR USE

Instrument power off
Always run a standby cycle before switching the power off if the instrument is
not going to be used before the following day, particularly the evening.
7KLVRSHUDWLRQDOORZVWKHÀXLGLFFLUFXLWVWREHULQVHGZLWKWKHULQVLQJVROXWLRQWRUHGXFHWKHULVN
caused by crystallization of the drying reagents.
'RQRWOHDYHWKHLQVWUXPHQWZLWKRXWZRUNLQJIRUPRUHWKDQGD\V)RUJRRGPDLQWHQDQFHMXVW
VZLWFKWKHDSSOLDQFHRQDQGZKHQWKHULQVLQJF\FOHVDUH¿QLVKHGUXQDVWDQGE\F\FOHDJDLQ
before switching off.
If the instrument is not going to be used for more than a week, run a full rinsing cycle with distilled
water (MENU/PRIME/RINSE/H²O RINSING).
Periodic checkup
- Check (once a week) the cleanliness inside the instrument especially the dilutor chambers and
the sample needle. If need be, clean them with distilled water and a soft cloth.
&KHFNLIWKHWZRFORWV¿OWHUVDUHGLUW\UHGFRORUHGGXHWRFRQVLGHUDEOHEORRGDFFXPXODWLRQ,I
yes, do not clean them. Replace with new ones (see following page).
In case of an aEQRUPDOLW\
:KHQDQRSHUDWLQJLQFLGHQWKDSSHQVGXULQJDGLOXWLRQF\FOHFKHFNWKHÀXLGLFFLUFXLWVEHIRUH
continuing, particularly the dilutor chambers to make sure no liquid remains at the bottom. If it
were the case, run a draining cycle (MENU/PRIME/RINSE/DRAINING) before continuing.
If the error occurs just after the sampling step, run a blank cycle (run a test without blood tube)
before running a new analysis.
Periodic cleaning
,Q RUGHU WR FOHDQ WKH ÀXLGLF FLUFXLWV WKRURXJKO\ LW LV UHFRPPHQGHG WR SHUIRUP WKH IROORZLQJ
cleaning operations:
- Put 3ml of cleaning solution (Ref +) into the RBC chamber.
3XWPORIFOHDQLQJVROXWLRQLQWRWKHGLOXWRUFKDPEHUV(23'/:DVWH6HHVHFWLRQ¿JXUH
- Wait 15 - 30 min.
- Run three blank cycles (5 part-diff without blood).
The periodicity of this intervention depends on the use frequency

of the device. The following rules may be respected:
- For 1000 cycles VHW: after each VHW.
DLB5
93
MAINTENANCE
8.2 REAGENT REPLACEMENT

2SHQWKHQHZUHDJHQWER[HVDVSUHYLRXVO\GHVFULEHGLQINSTALLATION chapter.
5HPRYH WKH 06&$5' IURP WKH QHZ 'LDGLII UHDJHQW

DQGreplaceLWZLWKthe RQHLQWKHDLB5
8QVFUHZDOOWKHFDSVRIWKHROGUHDJHQWVDQGUHPRYH
DOOtubes.
Never transfer / mix reagents

whether or not they are of the same
kind or batch.
Never reuse an empty reagent
container (batch, bag or bottle) or
partially empty, as this may distort
the results and / or damage the
machine.
4) Take each dip-tube one at a time and clean it with distilled

ZDWHUDQGDQRQÀXII\FORWKWKHQSODFHLQWRWKHQHZ
ERWWOHMatch colours between tubes and bottles.
5) Screw the caps of the new ERWWOHV onto the old bottles. Some
bottles may not be empty.
6) Do not forget to empty the waste container.
9HULI\WKHFORW¿OWHUVDQGGLOXWRUFOHDQOLQHVV&KDQJH¿OWHUV
if necessary.
8) From the screen, select ‘MENU/PRIME/RINSE/PRIMING

REAGENTS’ to prime reagents before running a new analysis.
DLB5
94
MAINTENANCE
8.3 BLOOD CLOT FILTERS

Filters are inserted on the two tubes connected to the dilutor chambers. They are used to stop
EORRGFORWVLQWKHÀXLGLFFLUFXLWWRDYRLGULVNVRIEORFNDJHVPDLQO\LQVLGHWKHFRXQWLQJDSHUWXUH
DQGYDOYHV
See the picture below.
,IWKH\KDYHWREHUHSODFHGWDNHFDUHWRIROORZWKHGLUHFWLRQLQGLFDWHGE\WKHDUURZRQWKH¿OWHU
body as depicted in the enlarged drawing. The arrow tip must always face towards the opposite
side of the dilutor chambers.
Picture 8.3.1
:DVWHFKDPEHU¿OWHU
(with black rings)
Eosino
FKDPEHU¿OWHU
(R¿OWHUGHSHQGLQJRQYHUVLRQ
PDL (Predilution)
FKDPEHU¿OWHU
Dilutor RBC Dilutor chambers: EO PDL Waste
7RFKDQJHD¿OWHUSXOOWKHÀH[LEOHWXEHWLJKWO\WRWDNHRIIWKHROG¿OWHUWKHQZHWWKHQHZ¿OWHUWR
make it easier to insert in the tube. The two black rings indicate the location of the waste circuit,
WKHWZRUHGULQJVWKHORFDWLRQRIWKH(2FLUFXLWDQGQRULQJWKHORFDWLRQRIWKH3'/FLUFXLWWRDYRLG
WXEHLQYHUVLRQGXULQJWKHZDVWHRU3'/¿OWHUFKDQJH
IMPORTANT
$IWHUFKDQJLQJD3'/FKDPEHU¿OWHULWLVQHFHVVDU\WRSHUIRUPDPLQLPXPRI
three blank counting cycles (run the analyses without the blood sampling tube)
LQRUGHUWRGHFRQWDPLQDWHWKHÀXLGLFFLUFXLWV
The message on the left indicates that a transfer anomaly

ANOMALY
ZKLFKFRXOGEHFDXVHGE\DGLUW\¿OWHUKDVEHHQGHWHFWHG
Predilution or measuring
chamber draining problem
Filter blockages may not be detected by the
&KHFNFORW¿OWHU system but it can cause other types of anomalies:
Repeated mixing errors during testing, too
Strike a key... much residual liquid at the bottom of the dilution
chambers...
DLB5
95
MAINTENANCE
8.4 UPDATING PROGRAM
It is highly recommended to ask your supplier for the

newest version of the sofware.
BEFORE ANY REPLACEMENT ACTION,

TURN THE DLB5 POWER OFF
WITH POWER CABLE UNPLUGGED.
An updating program requires the replacement of an

electronic component (memory). See photo on the
left. This component must be inserted into the correct
position. Incorrect setting can cause the destruction of
the component
Note the locating notch on the photo.
Locating notch
Printout calibration coefficients before updating
program (MENU/PARAMETERS/CALIBRATION).
Program memory access
Unscrew the lower screw of the 6ZLYHOWKHFORVLQJKDWFKDURXQGWRVHWLW

access hatch completely then LQWKHDERYHSRVLWLRQ
slightly loosen the upper screw Then, tighten the upper screw to maintain
(about 1 turn). the hatch in this position.
Lever
DLB5
96
MAINTENANCE
Replacing the memory program
NOTE THE DIRECTION OF THE COMPONENT LOCATED BY ITS NOTCH

Notch locating
Lever
3XOOXSWKHPHPRU\VRFNHWOHYHU
2) Pull out the original component. It should come out
easily.
3) Check that the pins of the new component are
straight.
4) Insert the new component into the socket and check
that:
- It is in the right way up (notch up)
- All the pins are correctly inserted into the socket.
:KLOHKROGLQJWKHFRPSRQHQWLQSODFH¿[LWLQWRWKH
VRFNHWE\SXOOLQJGRZQWKHOHYHU
Once the replacement has been carried out, close the

access hatch, plug the power cable and turn the power
on.
Note: in some cases, when the power is on the self-

WHVWF\FOHFDQGLVSOD\D¿[HGVFUHHQIRUPRUHWKDQWHQ
seconds.
IMPORTANT
There is a possibility that a change of program may cause the loss of
DQDO\VHVRUFDOLEUDWLRQFRHI¿FLHQWVGHSHQGLQJRQWKHXSJUDGLQJOHYHO
,QFDVHRIVXFKSRVVLELOLW\DVSHFLDOXSJUDGHSURFHGXUHZLOOEHSURYLGHG
WR JLYH WKH QHFHVVDU\ LQVWUXFWLRQV EHIRUH WKH UHSODFHPHQW RI WKH
PHPRU\SURJUDPWRDYRLGDFRPSOHWHUHFDOLEUDWLRQRIWKHDQDO\VHU
DLB5
97
MAINTENANCE
8.5 CHAMBER ACCESS
Cover wing screws

on WBC/EO measuring
chamber
7KHPHDVXULQJFKDPEHULVSURWHFWHGE\DPHWDOOLFFRYHU¿[HGLQSODFHE\WZRZLQJVFUHZV
If, for some reason, it is necessary to gain access (e.g. to change the counting aperture or
SKRWRPHWHUDFFHVVWDNHRIIWKHWZRVFUHZVDQGSXOOWKHFRYHUIRUZDUG
IMPORTANT
Be very careful not to pinch a tube when putting cover
EDFNDVWKLVFRXOGSUHYHQWWKHOLTXLGÀRZ
Never run an analysis without the measuring chamber

cover as this could alter the results.
8.6 AIR PUMP ACCESS
Cover screws
on air and
syringe pumps
Air pump
DLB5
98
9: EXPORT DATA
DLB5
99
EXPORT DATA
9.1 COMPUTER CONNECTION

Computer connection allows DLB5 data transmission to the main computer in order to store
DQDO\VLVUHVXOWVHYHQWXDOO\DGGSHUVRQDOFRPPHQWVDQGVWRUHWKHPLQDPHGLXPZLWKKLJKHU
storage capacity than the DLB5 memory.
9.1.1 Hardware connection

7RUHIHUVHFWLRQIRUWKHW\SHDYDLODEOHFRQQHFWLRQ5686%(WKHUQHW:L)L
9.1.2 Software connection

6HWWKHSDUDPHWHUVRIWKHGHYLFHFRPPXQLFDWLRQSRUWLQDFFRUGDQFHZLWKWKHUHTXLUHGIRUPDW
of the main computer (MENU/SYSTEM/COMMUNICATION then screen #2 COMPUTER).
9.1.3 OPERATING MODE

Two possibilities: on computer request or automatically / by user from the DLB5 .
Send on request
7KH'/%LVVWLOOLQZDLWPRGHUHDG\WRUHFHLYHWKHRUGHUVIURPWKHPDLQFRPSXWHUDWDQ\
point of the cycle.
- The main computer sends a command order to the DLB5 and must wait for the answer (there
is always an answer) to this order before sending another.
- If the texts sent by DLB5 are language-dependant (e.g. Blood Bank Name), they will be
WUDQVPLWWHGDFFRUGLQJWRWKHODQJXDJHVHOHFWHGRQWKHGHYLFH
- Each command sent by the main computer must begin with the $$ double character (ASCII: 36
decimal, 24 hexadecimal) and end by a carriage return (CR) (ASCII: 13 decimal, 0D hexadecimal).
,IWKLVEDVLFUXOHLVQRWUHVSHFWHGDFRPPXQLFDWLRQIDLOXUHFDQKDSSHQ7KHRQO\ZD\WRVROYH
WKHSUREOHPLVWRWXUQRIIWKHGHYLFH
- Each answer sent by the DLB5 begins with the # character (ASCII: 35 decimal, 23 hexadecimal)
and ends by a carriage return (CR).
- #E(CR) answer indicates misunderstanding of the command (bad format order).
- Possible answer #A(CR) common to all requests asking for results indicates that no analysis
is in memory.
,WLVDGYLVDEOHWRFKHFNWKHDQVZHUWREHVXUHWKDWLWLV2.VRWKHIROORZLQJLQIRUPDWLRQFDQEH
read.
7KH(7;HQGRIEORFNDQVZHUKDVDYDOXHRI
Convention for formats used hereafter
c = Alphanumeric character (e.g. 10c = 10 characters)

Q 6LJQL¿FDQWQXPEHUVSDFHVVWDQGLQIRUWKHRQWKHOHIW
1 6LJQL¿FDQWQXPEHUDOZD\VJLYHQ
_ = Always a space
DLB5
101
EXPORT DATA
9.2 REQUESTS
9.2.1 Software revision number request

Command
$$SP(CR)
Answers
#P(CR) Request OK
F111;V&5 µ)¶6RIWZDUHUHYLVLRQRQGLJLWV&5HJ)$V
c = F (Ascii: 70 decimal, 46 Hexadecimal) for DLB5 .
,WLVDGYLVDEOHWKDWWKLVUHTXHVWEHWKH¿UVWFRPPDQGVHQWE\DQDSSOLFDWLRQ
D 7KLVFRQ¿UPVDJRRGFRQQHFWLRQEHWZHHQWKHLQVWUXPHQWDQGWKHFRPSXWHU
E 7KLVDOORZVWKHFRUUHFWPDQDJHPHQWRIWKHIXWXUHXSJUDGHRIWKHUHTXHVWV
9.2.2 Set decimal separator for decimal results

Command
$$Vs(CR)
s: can take characters ‘.’ (point -> ASCII: 46 decimal, 2E Hexadecimal) or
‘,’ (comma -> ASCII 44: decimal, 2C Hexadecimal)
Answer
#V(CR) Request OK
By default, the decimal separator is the one from the selected language. To modify it, send this
command before making requests to send out numeral results.
9.2.3 Set character string receiving format

Commands
$$CA(CR) All data is transmitted in character mode ASCII 0..127. (Default mode)
'/%FKDUDFWHUVDERYHDUHDSSUR[LPDWHWRPDWFKDVZHOODV
possible
$$CB(CR) All characters >127 are transmitted in DOS format.
$$CC(CR) All characters >127 are transmitted in WINDOWS format
Answer
#C(CR) Request OK
7KHDERYHWZRFRPPDQGVPXVWEHSHUIRUPHGLQWKHDSSOLFDWLRQEHIRUHIROORZLQJRWKHUUHTXHVWV
7KHVHVHWWLQJVDUHORVWZKHQWKHGHYLFHLVWXUQHGRII
DLB5
102
EXPORT DATA
9.2.4 Stored analyses number request

Command
$$AM(CR)
Answers
#M(CR) Request OK
NNN(CR) Number of analyses in the DLB5 memory (Range 0..250*)
2QO\IRU'/%HTXLSSHGZLWKDPRWKHUERDUGUHYRUKLJKHU5DQJHZLWKROGHUUHY
/DVWDQDO\VLVVSHFL¿FDWLRQUHTXHVW
Command
$$AL(CR)
Answers
#A(CR) No analysis in memory on DLB5
#L(CR) Request OK
)ROORZVSHFL¿FDWLRQDVGH¿QHGEHORZ
ETX
Sequence number NNNN(CR)

5HVHUYHGXVH 11&5
Blood bank number NN(CR) 01 to 14
%ORRGEDQNQDPH F&5 /HIWMXVWL¿HGVSDFHV
Day NN(CR)
Month NN(CR)
Year NNNN(CR)
Hour NN(CR) Always 24 hours format
Minute NN(CR)
File 20c(CR)
Patient 20c(CR)
Comment 20c(CR)
!FKDUDFWHUVE\VSHFL¿FDWLRQEORFN
*** Transmitted in the language selected on the DLB5 .
DLB5
103
EXPORT DATA
6SHFL¿FDWLRQRIDQDQDO\VLVE\LWVVHTXHQFH
number
Command
$$ASssss(CR) ssss = 4-digit sequence number (e.g.: 0081)
Answers
#N(CR) No analysis in memory for this sequence number
#S(CR) Request OK
)ROORZ$QDO\VLVVSHFL¿FDWLRQGHVFULEHGDERYH
ETX
Comment
$VWKHVHTXHQFHQXPEHUVDUHFODVVL¿HGLQLQFUHDVLQJRUGHUH[FHSWIROORZHGE\
WKHODVWDQDO\VLVVSHFL¿FDWLRQDOORZVWKHODVWVHTXHQFHQXPEHUWREHNQRZQDQGWKHSUHYLRXV
ones to be deduced. This allows data to be sent out in small size blocks.
$OOVWRUHGDQDO\VHVVSHFL¿FDWLRQUHTXHVW
Command
$$AT(CR)
Answers
#T(CR) Request OK
111&5 1XPEHURIIROORZLQJDQDO\VHVVSHFL¿FDWLRQ
6HQGLQJRIWKH111VSHFL¿FDWLRQV
ETX
CAUTION
2QO\WKHODVWDQDO\VHVDUHPDQDJHGHYHQLIDQDO\VHVDUHVWRUHGLQVLGHWKHPHPRU\
,WLVQRWUHFRPPHQGHGWRXVHWKLVUHTXHVWDQDO\VHVLQWKHGHYLFHPHPRU\LPSO\PRUH
WKDQ.FKDUDFWHUVWREHVHQW,WLVSUHIHUDEOHWRXVHWKHSUHYLRXVO\GHVFULEHGPHWKRGWRUHDG
analysis one at a time.
6SHFL¿FDWLRQRIDOOWKHDQDO\VHVIRUDVSHFL¿F
day
Command
$$ADdd/mm/yyyy(CR) (day / month / year)
Answers
#N(CR) No analysis in memory for this day
#D(CR) Request OK
111&5 1XPEHURIIROORZLQJDQDO\VHVVSHFL¿FDWLRQ
ETX
DLB5
104
EXPORT DATA
6SHFL¿FDWLRQRIDOOWKHDQDO\VHVIRUDGDWH
EH\RQGWKDWVSHFL¿FGD\
Command
$$APdd/mm/yyyy(CR) (day / month / year)
Answers
#N(CR) No analysis in memory beyond this day
#D(CR) Request OK
111&5 4XDQWLW\RIIROORZLQJDQDO\VHVVSHFL¿FDWLRQ
ETX
1RWH,IVHYHUDODQDO\VHVVSHFL¿FDWLRQVDUHVHQWRXWWKH\DUHLQFKURQRORJLFDORUGHU7KH¿UVW
sent out is the one of the last analysis done in the requested selection.
+LVWRJUDPVE\DGH¿QHGVHTXHQFHQXPEHU
A. THR/RBC/WBC histograms
Command
$$HGssss(CR)
Answers
#G(CR) Request OK
+LVWRJUDPVVHQWRXWDFFRUGLQJWRDULJKWMXVWL¿HGFKDUDFWHU¿[HGIRUPDW
Synchronization THR(CR)
4XDQWLW\RI7+5IRUYROXPH 111&5
.../...
4XDQWLW\RI7+5IRUYROXPH 111&5
Synchronization RBC(CR)
4XDQWLW\RI5%&IRUYROXPH 111&5
.../...
4XDQWLW\RI5%&IRUYROXPH 111&5
Synchronization WBC(CR)
4XDQWLW\RI:%&IRUYROXPH 111&5
.../...
4XDQWLW\RI:%&IRUYROXPH 111&5
Position of MonoStart cursor NNN(CR)

Position of MonoEnd cursor NNN(CR)
ETX
DLB5
105
EXPORT DATA
B. EO histogram
Command
$$HEssss(CR)
Answers
;&5 1R(2KLVWRJUDPDYDLODEOHIRUWKLVDQDO\VLV
#G(CR) Request OK
+LVWRJUDPVVHQWRXWDFFRUGLQJWRDULJKWMXVWL¿HGFKDUDFWHU¿[HGIRUPDW
Synchronization EOS(CR)
4XDQWLW\RI(2IRUYROXPH 111&5
.../...
4XDQWLW\RI(2IRUYROXPH 111&5
ETX
1RWH+LVWRJUDPVDUHVHQWZLWKVPRRWKLQJFRHI¿FLHQWVXVHGIRUGLVSOD\LQJUHVXOWV
+DHPDWRORJ\UHVXOWVE\DGH¿QHGVHTXHQFH
number
A. Results alone
Command
$$HBssss(CR)
Answers
#H(CR) Request OK
6HQGLQJRIKDHPDWRORJ\UHVXOWVGLJLW¿[HGIRUPDWFHQWHUHGRQGHFLPDOSRLQW&5
(20 lines of 8 characters / line)
ETX
B. Results and units
Command
$$HUssss(CR)
Answers
#H(CR) Request OK
6HQGLQJRIKDHPDWRORJ\UHVXOWVXQLWVGLJLW¿[HGIRUPDWVSDFHOHIWMXVWL¿HGYDOXH
right spaces) + CR
ETX
DLB5
106
EXPORT DATA
C. Test name and results
Command
$$HCssss(CR)
Answers
#H(CR) Request OK
6HQGLQJRIKDHPDWRORJ\WHVWVQDPHOHIWMXVWL¿HGGLJLW¿[HGIRUPDWULJKWVSDFHV
results
+ CR
ETX
D. Test name, results and units
Command
$$HAssss(CR)
Answers
#H(CR) Request OK
Sending of haematology tests name + results + units + CR
ETX
DLB5
107
EXPORT DATA
E. Haematology results format
Std Unit SI Unit IU Unit

Test Result Unit Result Unit Result Unit
WBC_ _nnN.NN _m/mm3_ _nnN,NN _m/mm3_ _nnN,NN _m/mm3_ (CR)

Lym. __nN.N_ _%_____ __nN,N_ _%_____ __nN,N_ _%_____ (CR)
Mon. __nN.N_ _%_____ __nN,N_ _%_____ __nN,N_ _%_____ (CR)
Neu. __nN.N_ _%_____ __nN,N_ _%_____ __nN,N_ _%_____ (CR)
Eo._ __nN.N_ _%_____ __nN,N_ _%_____ __nN,N_ _%_____ (CR)
Ba._ __nN.N_ _%_____ __nN,N_ _%_____ __nN,N_ _%_____ (CR)
RBC_ __nN.NN _M/mm3_ __nN,NN _M/mm3_ __nN,NN _M/mm3_ (CR)
Hct_ __nN.N_ _%_____ __nN,N_ _%_____ ___0,NN _______ (CR)
0&9B BQQ11B BÀBBBB BQQ11B BÀBBBB BQQ11B BÀBBBB &5
MCH_ __nN.N_ _pg____ __nN,N_ _fmol__ __nN,N_ _pg____ (CR)
MCHC __nN.N_ _g/dl__ __nN,N_ _mmol/l _nnN___ _g/l___ (CR)
RDW_ __nN.N_ ______ __nN,N_ ______ __nN,N_ ______ (CR)
THR_ nnnN___ _m/mm3_ nnnN___ _m/mm3_ nnnN___ _m/mm3_ (CR)
Pct_ __nN.NN _%_____ __nN,NN _%_____ __nN,NN _%_____ (CR)
039B BBQ11B BÀBBBB BBQ11B BÀBBBB BBQ11B BÀBBBB &5
PDW_ __nN.N_ _______ __nN,N_ _______ __nN,N_ _______ (CR)
Hb__ __nN.N_ _g/dl__ __nN,N_ _mmol/l _nnN___ _g/l___ (CR)
Err1 iabcdef _______ iabcdef _______ iabcdef _______ (CR)
Err2 rnmkghj _______ rnmkghj _______ rnmkghj _______ (CR)
Err3 tuvsopq _______ tuvsopq _______ tuvsopq _______ (CR)
*Test :
ATTENTION! Test name depends on localization/country setting (see section 4.4.3).
i.e. WBC_ is replaced by GB__ in french, by LEU_ in german, and so on...
(UU(DFKFKDUDFWHUFDQWDNHµ¶1RHUURURUµ¶(UURUZLWK$ODUP0DUNUHPLQGHUYDOXH

a: Small Blockage on THR channel (&) g: THR Mixing/Homogenization (@)
b: Small Blockage on RBC channel (&) h: RBC Mixing/Homogenization (@)
c: Small Blockage on WBC channel (&) j: WBC Mixing/Homogenization (@)
d: Photometer or Hb Error (E) k: Big Blockage on THR channel (#)
e: WBC population Error (L/M/I) m: Big Blockage on RBC channel (#)
f: Not Used, Always ‘0’ n: Big Blockage on WBC channel (#)
i: Thalassemia possibility (T) r: Analysis rejected (MCHC Pb) (R)
s: EO Mixing/Homogenization (@) t: ‘0’ if 5POP analysis, ‘1’ if 3POP
o: Eosino Error (E) u: Not Used, Always ‘0’
S 6PDOO%ORFNDJHRQ(2FKDQQHO Y %DVRUHMHFWHG5
q: Big Blockage on EO channel (#)
Note 1: 7KHWHVWQDPHVZLOOEHVHQWLQWKHODQJXDJHVHOHFWHGRQWKH'/%WKHYDOXHIRUPDW
and unit according to the Hb Unit selected in the APPLICATION menu (MENU/SYSTEM/
APPLICATION/UNITS).
Note 2: ,IWHVWVDUHSHUIRUPHGLQ3DUW0RGHUHVXOWVDUHVHQWZLWKWKHVDPHDERYHIRUPDWZLWK
Eo. and Bas. = 0 and Err3 = 100 0000.
DLB5
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EXPORT DATA
9.2.12 Haematology normal range by a blood bank number

Command
$$HSNN(CR)
NN represents the blood bank number (01 to 06)

(See section 6.1.1)
Answers
$&5 1REORRGEDQNIRUWKHVSHFL¿HGQXPEHU
#S(CR) Request OK
6HQGLQJRIQRUPDOUDQJHVGLJLW¿[HGIRUPDWFHQWHUHGRQGHFLPDOSRLQWIRU0LQYDOXHVµ¶
GLJLWVFHQWHUHGRQGHFLPDOSRLQWIRU0D[YDOXHV&5
ETX
Std / SI Unit IU Unit

WBC _nnN.N_-_nnN.N_ _nnN.N_-_nnN.N_ (CR)
Lympho __nN.N_-__nN.N_ __nN.N_-__nN.N_ (CR)
Mono __nN.N_-__nN.N_ __nN.N_-__nN.N_ (CR)
Neutro __nN.N_-__nN.N_ __nN.N_-__nN.N_ (CR)
Eosino __nN.N_-__nN.N_ __nN.N_-__nN.N_ (CR)
Baso _______-_______ _______-_______ (CR)
RBC __nN.N_-__nN.N_ __nN.N_-__nN.N_ (CR)
Hct __nN.N_-__nN.N_ ___0.NN-___0,NN (CR)
MCV _nnN.N_-_nnN.N_ _nnN.N_-_nnN.N_ (CR)
MCH __nN.N_-__nN.N_ __nN.N_-__nN.N_ (CR)
MCHC __nN.N_-__nN.N_ _nnN___-_nnN___ (CR)
RDW __nN.N_-__nN.N_ __nN.N_-__nN.N_ (CR)
THR nnnN___-nnnN___ nnnN___-nnnN___ (CR)
Pct _______-_______ _______-_______ (CR)
MVP __nN.N_-__nN.N_ __nN.N_-__nN.N_ (CR)
PDW __nN.N_-__nN.N_ __nN.N_-__nN.N_ (CR)
Hb __nN.N_-__nN.N_ _nnN___-_nnN___ (CR)
Normal ranges are sent in the same order as haematology results with the format and
YDOXHVFRUUHVSRQGLQJWRWKHHb Unit selected in the APPLICATION menu (MENU/SYSTEM/
APPLICATION/UNITS).
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EXPORT DATA
9.3 AUTO / MANUAL TRANSMISSION

5HVXOWVFDQEHVHQWIURP'/%HLWKHUDXWRPDWLFDOO\DWWKHHQGRIDYDOLGDQDO\VLVVHH0(18/
SYSTEM/COMMUNICATION), or manually when an analysis is displayed. In the latter case,
press the Up Arrow key when the main results screen is displayed (or F5 Function key with
an external keyboard from any analysis screen displayed) to make appear the following screen:
File format: (1 or 2) same as Auto 1, Auto 2 options in

TRANSMIT RESULTS communication menu.
When a Quality Control analysis is performed, this line is

File format : 1 not displayed because no histogram is sent in this mode.
'DWD IRUPDW LV LGHQWLFDO WR VRPH RSWLRQV SUHYLRXVO\

Transmit
described in the communication mode on request.
Cancel
A. Regular analysis data format
1) Decimal separator always a period (same as $$V. command).

&KDUDFWHUVLQ$6&,,VDPHDV&$FRPPDQG
3) Start character is the control character STX (2).
6HQGLQJRIDQDO\VLVVSHFL¿FDWLRQVDPHDV$/FRPPDQGFKDUDFWHUV
5) Sending of analysis results with same format as $$HA command: 380 characters.
6) Sending of normal range of the displayed bank (same as $$HSnn command): 272 characters.
- If File format = 1 or selection = Auto 1: step 7 skipped).
7) Sending of histograms (same as $$HGssss command): 3092 characters.
(2KLVWRJUDPLVQHYHUVHQWDXWRPDWLFDOO\
8) Ends by the control character ETX (3) sending.
DLB5
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EXPORT DATA
B. Control Quality analysis data format
1) Decimal separator always a period (same as $$V. command).

&KDUDFWHUVLQ$6&,,VDPHDV&$FRPPDQG
3) Start character is the control character STX (2).
6HQGLQJRIDQDO\VLVVSHFL¿FDWLRQVDPHDV$/FRPPDQGEXWZLWKWKH¿HOGVFRQWHQWGHVFULEHG
at the following page): 102 characters.
5) Sending of analysis results with same format as $$HA command: 380 characters.
If the analysis is recalled from memory, only the 6 parameters recorded and some computed
SDUDPHWHUVZLOOEHVHQWZLWKDVLJQL¿FDQWYDOXHWKHRWKHUSDUDPHWHUVZLOOEHQXOO
6) Sending of normal range of the displayed bank (same as $$HSnn command): 272 characters.
2QO\WKHQRUPDOUDQJHVHQWHUHGLQWKH&RQWURO%ORRGOHYHOVFUHHQDQGUHFRUGHGZLWKWKHDQDO\VLV
ZLOOEHVHQWZLWKDVLJQL¿FDQWYDOXHRWKHUVQRUPDOUDQJHVSDUDPHWHUVZLOOEHQXOO
7) Ends by the control character ETX (3) sending.
& &RQWURO4XDOLW\DQDO\VLVVSHFL¿FDWLRQIRUPDW
Sequence number NNNN(CR)

Number of lines of haematology results NN(CR) **a) - (17)
Blood bank number NN(CR) **b) - 15 to 17
%ORRGEDQNQDPH F&5 F/HIWMXVWL¿HGVSDFHV
Day NN(CR)
Month NN(CR)
Year NNNN(CR)
Hour NN(CR) Always 24 hour format
Minute NN(CR)
Lot number / Expiry Date 20c(CR) **d)
Patient 20c(CR) 2 characters + 18 spaces
Comment 20c(CR)
!FKDUDFWHUVE\VSHFL¿FDWLRQEORFN
**a) The total number of lines sent with the results block contain 3 additional lines for error
indicators
6HHµ+DHPDWRORJ\UHVXOWVIRUPDW¶SUHYLRXVO\
E /RZOHYHO&RQWURO%ORRG
1RUPDOOHYHO&RQWURO%ORRG
+LJKOHYHO&RQWURO%ORRG
F 1DPHRIWKHOHYHOWKLVLVWKH&RQWURO%ORRGEDQN
Transmitted in the language selected on the DLB5
G /RW1XPEHU)RUPDWQQQQQ/HIWMXVWL¿HG
- 4 spaces,
- Expiry Date: mm/dd/yyyy or dd/mm/yyyy formats according to the selected language
DLB5
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IMPORTANT
Waste disposal of electrical and electronic
equipment:
This device must be eliminated according to
the current legislation of the country where it
was used.
DLB5
112
10: APPENDIX
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APPENDIX - 1
10.1 DLB5 MAIN FEATURES

10.1.1 Main medical features
Measured parameters : WBC, Eo#, RBC, THR, Hb, Hct
Calculated parameters : Lym%, Lym#, Mon%, Mon#, Neu%, Neu#, Eo%, Ba%
: MCV, MCH, MCHC, RDW
: MPV, Pct, PDW
Histograms : WBC, RBC, THR (Displayed and printed out)
: EO (Displayed and printed out on built-in printer only)
Min/max range alarms : Programmable, displayed and printed out
Whole blood sample : 10 to 30 μl. Programmable dilution rate
Analysis time : 3 part-diff 60 WHVWV/hour
: 5 part-diff 40 WHVWV/hour
10.1.2 Main software features

Language : French, English + 3rd customized language on request
Application : 6 Blood banks completely programmable
Storage : Up to 250 analyses in safeguard memory
: Up to 125 Quality Control results in safeguard memory
Q.C. : Q.C. graph (L. J. type) for WBC, RBC, THR, MCV and Hb
Display range : WBC 0.00 to 655.35 m/mm3

: Lym. 0.0 to 99.9 % (0.00 to 655.35 m/mm3)
: Mon. 0.0 to 99.9 % (0.00 to 655.35 m/mm3)
: Neu. 0.0 to 99.9 % (0.00 to 655.35 m/mm3)
: Eos. 0.0 to 50.0 % (0.00 to 325.00 m/mm3)
: RBC 0.00 to 99.99 M/mm 3
: RDW 0.0 to 99.9

: Hct 0.0 to 99.9 % (0.0 to 0.99 with IU units)
: MCH 0.0 to 999.9 pg (0.0 to 99.9 fmol with SI units)
: MCHC 0.0 to 99.9 g/dl (0 to 999 mmol/l with SI units)
: THR 0 to 9999 m/mm 3
039 WR À

: Pct 0.00 to 9.99 %
: PDW 0.0 to 99.9
: Hb 0.0 to 99.9 g/dl (0.0 to 99.9 g/l with IU units)
: Hb (0 to 999 mmol/l with SI units)
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APPENDIX - 1
10.1.3 Mechanical characteristics

General
Dimensions : Width 210 mm, height 340 mm, depth 250 mm

Weight : 13 Kg
External Power supply : 100-240 Volt / 50-60 Hz 2.0 A (automatic commutation)
: 24 VDC 6.25A
(QYLURQPHQW 5RRPWHPSHUDWXUH&)!5HDJHQWVVSHFL¿FDWLRQ
: Humidity: 90% maximum with no condensation
Display : Graphic color TFT LCD 320 x 240 dots
.H\ERDUG %XLOWLQVHQVLWLYHNH\V
: External PC type keyboard (optional)
%DUFRGHUHDGHUIRULGHQWL¿FDWLRQRSWLRQDO
Printer : Built-in thermal printer 24 columns / 384 dots
: External parallel or USB printer (optional)
Connections : 1 serial RS-232 (1200 to 57600 bauds) for computer connection
VHULDO56WREDXGVIRUUHVHUYHGXVH
: 1 parallel for printer
: 1 USB for printer
: 1 USB for computer connection
: 1 Ethernet for computer connection (optional)
:L¿IRUFRPSXWHUFRQQHFWLRQRSWLRQDO
: 1 PS/2 mini-DIN 6 pin for keyboard and/or barcode reader
Dilutor, counter
Dilutor : Sequential dilution controlled step by step

Motors : 3 stepping motors, 2 motors DC (pumps)
9DOYHV ÀLSÀRSYDOYHVZDUUDQW\PLOOLRQF\FOHV
Sample needle : Automatic internal and external cleaning
&RXQWLQJW\SH (OHFWULF¿HOGDFURVV
- an 80 μm aperture for WBC and EO
- an 60 μm aperture for RBC and THR
Hb measurement : Stabilized reduced Hb with photometry at 540 nm
Others
$FRXVWLFOHYHO GE
)XQFWLRQLQJDOWLWXGH PIRUDOWLWXGH!PFRQVXOWXV
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APPENDIX - 1
10.1.4 Performance characteristics
/LPLWRITXDQWL¿FDWLRQ WBC 0,2 x 103/mm3

PLT 15 x 103/mm3
Linearity WBC 0,2 to 1,5 x 103/mm3 : +/- 0,2 x 103/mm3

(For high and low 10 to 100 x 103/mm3 : +/- 10%
concentrations) 100 to 200* x 103/mm3 : +/- 15%
RBC 0,2 to 2 x 106/mm3 : +/- 0,2 x 106/mm3

5 to 9 x 106/mm3 : +/- 5%
HGB 0,5 to 5 g/dl: +/- 0,5 g/dl

17 to 25 g/dl: +/- 5%
PLT 15 to 60 x 103/mm3 : +/- 15 x 103/mm3

450 to 1000 x 103/mm3 : +/- 15%
Correlation-Linearity LEU R* 0,98 within slope = 1 +/- 0,05

(Correlation factors LYM R * 0,95 within slope = 1 +/- 0,15
obtained by comparing Mono R * 0,60 within slope = 0,75 +/- 0,25
WKHPHVXUHGYDOXHVDJDLQVW Neu R * 0,95 within slope = 1 +/- 0,15
a reference instrument) Eos R * 0,80 within slope = 1 +/- 0,25
RBC R * 0,98 within slope = 1 +/- 0,10
Hgb R * 0,98 within slope = 1 +/- 0,05
VGM R * 0,90 within slope = 1 +/- 0,10
Hct R * 0,98 within slope = 1 +/- 0,05
PLT R * 0,98 within slope = 1 +/- 0,10
Carry-over WBC )1,0%

RBC )1,0%
PLT ) 1,0%
VGM )1,0%
Hct ) 1,0%
Hgb ) 1,0%
*
With automatic dilution x2 if WBC result is higher than 100 103/μl.
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APPENDIX - 1
Precision WBC 1,5 to 4 x 103/mm3 : ) 4,0%

Repeatability up to 4 x 103/mm3 : ) 3,0%
RBC 2 to 4 x 106/mm3 : ) 2,5%

up to 4 x 106/mm3 : ) 2,0%
PLT 60 to 150 x 103/mm3 : )10,0%

150 to 450 x 103/mm3 : ) 5,1%
up to 450 x 103/mm3 : ) 5,0%
VGM WRÀ : )1,0%

WRÀ) 1,0%
XSWRÀ : ) 1,0%
Hct 15 to 30% : ) 2,5%

up to 30% : ) 2,0%
Hgb 5 to 10 g/dl : ) 2,5%

up to 10 g/dl : ) 2,0%
EOS below 0,4 x 103/mm3 : ) 35,0%

up to 0,4 x 103/mm3 : )15,0%
Precision WBC 1,5 to 4 x 103/mm3 : )5,0%

Reproductibility 4 to 10 x 103/mm3 : ) 4,0%
up to 10 x 103/mm3 : )3,0%
RBC 2 to 4 x 106/mm3 : ) 3,5%

4 to 5 x 106/mm3 : ) 2,1%
up to 5 x 106/mm3 : ) 2,0%
PLT 60 to 150 x 103/mm3 : ) 14,0%

150 to 450 x 103/mm3 : ) 6,8%
up to 450 x 103/mm3 : )5,0%
VGM WRÀ : ) 1,0%

WRÀ) 1,0%
XSWRÀ : ) 1,0%
Hct 15 to 30% : )3,5%

30 to 40% : ) 2,4%
up to 40% : ) 2,0%
Hgb 5 to 10 g/dl : ) 3,5%

10 to 17 g/dl : ) 2,1%
up to 17 g/dl : ) 2,0%
EOS below 0,4 x 103/mm3 : ) 35,0%

up to 0,4 x 103/mm3 : )15,0%
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APPENDIX - 2
10.2 NORMAL RANGE
1RUPDOUDQJHRUUHIHUHQFHUDQJHLQFRUSRUDWHGLQWKH'/%DQDO\VHUFDQEHPRGL¿HGWRPDWFK
laboratory population met on a daily basis use (See section 4.3.2 : 6 to 15th screen : Normal
range).
([SHFWHGQRUPDOUDQJHFDQLQGHHGYDU\DFFRUGLQJWRGLIIHUHQWIDFWRUVVXFKDVJHQGHUDJH
dietary pattern, geographical location and so on.
Human haematology reference range

PARAMETER MALE FEMALE NEW BORN UNITS
WBC 4,0-10,0 4,0-10,0 3,5-11,0 m/mm3
Lym% 15,0-40,0 20,0-40,0 20,0-40,0 %
Mon% 1,0-10,0 1,0-10,0 1,0-10,0 %
N/G% 30,0-70,0 30,0-70,0 30,0-70,0 %
Eo% 0,0- 7,0 0,0- 7,0 0,5- 8,5 %
RBC 4,0- 5,9 3,8- 6,0 4,0- 5,5 M/mm3
Hct 35,0-54,0 33,0-54,0 35,0-45,0 %
0&9 À
MCH 25,0-33,0 25,0-32,0 35,0-45,0 pg
MCHC 28,0-36,0 28,0-36,0 28,0-36,0 g/dl
RDW 8,0-12,0 8,0-12,0 8,0-12,0
THR 150 -450 100 -450 150 -450 m/mm3
039 À
PDW 6,0-10,0 6,0-10,0 6,0-10,0
Hb 12,0-18,0 10,0-16,5 11,0-18,5 g/dl
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$33(1',;
10.3 3 PART-DIFF. CALIBRATION

The main WBC population cells are placed as follows:
Mono.
Lympho. Granulo.
Low threshold High threshold
7KHµ0212¶DUHDLVGH¿QHGE\ERWKMono Start and Mono End programmable pointers (MENU/

PARAMETERS/MODIFY/3rd screen).
The Lympho counting is done in the area between the low threshold (LowT) and the left Mono
pointer (Mono Start).
The Mono counting is done in the area between the left Mono pointer (Mono Start) and right
Mono pointer (Mono End).
The Granulo counting is done in the area between the right Mono pointer (Mono End) and the
high threshold (Hi.T).
3URJUDPPLQJYDOXHVDUHZRUNLQJRQDIXOOVFDOHRIWR
To calibrate the WBC 3 Part Differential, it is just necessary to place the ‘Mono’ (monocytes)
SRLQWHUVLQWKHYDOOH\EHWZHHQWKHO\PSKRF\WHVDQGJUDQXORF\WHVSHDNV,WLVYHU\LPSRUWDQWWR
UHVSHFWIRUWKHGHYLFHSDUWGLIIFDOLEUDWLRQWKHIROORZLQJSRLQWV
- EDTA K3 anticoagulant,
)LOOXSWKHZKROHEORRGWXEHZLWKGH¿QHGEORRGTXDQWLW\
- Perfectly mix the whole blood for WBC stabilization and homogenization,
- Analyze the blood in the 4 hours following the patient sampling.
The monocytes quantity can be calibrated by increasing or decreasing the space between both
cursors of the counting area.
WARNING
The leukocyte formula is usually not calibrated out of the ranges set
by the manufacturer. Because Haematology is a subjective science,
a different calibration of this formula is only useful to those whose
results do not match with the ones proposed by default.
DLB5
120
$33(1',;
Incorrect calibration: ‘Mono’ area is shifted to the left

Mono.
Consequence: Lympho results too low and Granulo results too high. Incorrect monocyte results.
Incorrect calibration: ‘Mono’ area is shifted to the right

Mono.
Consequence: Lympho results too high and Granulo results too low. Incorrect monocyte results.
Incorrect calibration: ‘Mono’ area too large
Mono.
Consequence: Lympho and Granulo results too low. Monocyte results too high.
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APPENDIX - 4
10.4 CONNECTORS
Pin Out
FRQQHFWRUVDUHDFFHVVLEOHRQWKHULJKWVLGHRIWKHGHYLFH
Parallel (//) printer / USB printer

7KHSLQIHPDOHFRQQHFWRULVSURYLGHGIRUDSDUDOOHOSULQWHUDQGXVHDVWDQGDUG3&SDUDOOHO
printer cord.
7KH86%FRQQHFWRULVSURYLGHGIRUDQG21/<)25DSULQWHUDQGXVHDVWDQGDUG3&86%SULQWHU
cord. Do not try to use both connectors (// and USB) at the same time.
The standard pin out are not described here.
Keyboard
7KHSLQ36FRQQHFWRULVSURYLGHGIRUDQH[WHUQDO3&W\SHNH\ERDUG7KHVWDQGDUGSLQRXW
is not described here.
Computer serial connections

3URYLGHGE\'6XEPDOHIHPDOHFRQQHFWRUVZLWKWKHSLQRXWDVEHORZ7KHGLDJUDPUHSUHVHQWV
the pin side of DLB5 .
1RWH7KH9RXWSXWLVRIDYHU\ORZLQWHQVLW\DQGLVSURYLGHGIRUVSHFLDOH[WHUQDO
LQWHUIDFH'RQRWXVHLWZLWKRXWWKHDSSURYDORI'LDODE*PE+
Motherboard V1.3 or before
1
6 DTR
2 TXD Motherboard V1.4 or higher
Reserved use 7 CTS 15 pin HDD-SUB male
9 pin D-SUB
3 RXD
male
8 RTS GND2
6
RTS2
4
RTS1
9 +12V 11 7 1 TXD1
5 GND +12V
12 8 2 RXD1
RXD2
13 9 3 GND1
TXD2
14 10 4 DTR1
5 GND DTR2
9 15 5 CTS1
4 DTR CTS2
Computer 8 CTS
9 pin D-SUB
3 TXD
female [[[ 5HVHUYHG XVH
7 RTS xxx2 : Computer
2 RXD
6
1
DLB5
122
$33(1',;
Computer USB connections (Motherboard V1.4 or higher)

)HPDOH86%%FRQQHFWRUSURYLGHGWRFRQQHFW'/%ZLWKD3&'ULYHUVUHTXLUHG
Computer Ethernet connections (Optional) (Motherboard V1.4 or higher)

(WKHUQHWFRQQHFWRUSURYLGHGWRFRQQHFW'/%RQQHWZRUN
LED connections status (Motherboard V1.4 or higher)

6WDWXVRIDFWLYHFRPSXWHUFRQQHFWLRQVRQ'/%
Off : Serial (RS232).
Green : USB.
Red / Amber : Ethernet (Wired or Wi-Fi, Wi-Fi in priority).
10.5 PC CONNECTION
PC Connection cable with 9 pins connector (Motherboard V1.3 or before)
DLB5 : Male DB9 PC: Female DB9
2 3
3 2
4 6
5 Shield Ground 5
7 8
8 7
PC Connection cable with 15 pins connector (Motherboard V1.4 or higher)
DLB5 : Female HDDB15 PC : Female DB9
8 3
9 2
10 6
11 Shield Ground 5
12 8
13 7
Cord dedicated for PC computer connection to DLB5
DLB5
123
APPENDIX - 6
10.6 METHODE LIMITATION - INTERFERENCES

Specimen Requirements
The anticoagulant tested and recommanded is K3 EDTA plastic or glass tubes.

Other anticoagulants can generate inaccurate results.
Blood should be assayed within 4 hours if stored at room temperature (20°C... 25°C) and 24
hours if stored between 2°C... 6°C.
Sample tubes
$Q\VL]HRIVDPSOHWXEHFDQEHXVHGZLWKDQ'/%LILWKDVEHHQFRUUHFWO\¿OOHGDFFRUGLQJWR
WKHPDQXIDFWXUHULQVWUXFWLRQV9DULRXVUHGXFHUVDUHDYDLODEOHWR¿WLQWRWKHWXEHVL]H
Environmental Factors
The instrument should be operated at room temperature (20°C... 25°C) and reagents should
be stored at temperatures between 15°C... 25°C. Reagents in use should be kept at room
temperature.
Do not install the instrument or reagent pack behind a window exposed to sunlight.
Biological interferences
Sickled red blood cells may not be accurately recognized and produce erroneous results.
Samples with cold agglutinins may falsely decrease the red blood count6. The indices will
LQGLFDWH WKDW WKH KHPRJORELQ DQG KHPDWRFULW YDOXHV GR QRW DJUHH $ PDQXDO HYDOXDWLRQ
of a blood smear is recommended. These samples should be incubated for 30 minutes
at 37°C and reassayed.
Fragmented erythrocytes can not be detected and lead to error in results: the number
RI UHG EORRG FHOOV LV DUWL¿FLDOO\ ORZ WKDW RI SODWHOHWV LV DUWL¿FLDOO\ HOHYDWHG DQG 9*0
DQG +HPDWRFULW DUH IDOVHO\ ORZ $ PDQXDO HYDOXDWLRQ RI D EORRG VPHDU FDQ GHWHFW WKH
source of errors5.
3DWLHQW VDPSOHV ZLWK QXFOHDWHG UHG EORRG FHOOV PD\ IDOVHO\ HOHYDWH WKH ZKLWH EORRG
count5,7. Additionally, the presence of these nucleated cells may interfere with the white
FHOO GLIIHUHQWLDO 7KH LQVWUXPHQW ZLOO JLYH DQ ©/ª HUURU FRGH LI WKH SUHVHQFH RI 15%&V
LV VXVSHFWHG $ PDQXDO HYDOXDWLRQ RI D EORRG VPHDU PXVW EH SHUIRUPHG
Platelet clumps and neonatal samples may interfere with the Drabkins method of
KHPRJORELQ GHWHUPLQDWLRQ $ PDQXDO HYDOXDWLRQ RI D EORRG VPHDU FDQ GHWHFW WKH VRXUFH
of errors3.
Samples with extreme lipemia, chylomicrons or extremely high bilirubin concentrations

PLJKW SURGXFH IDOVHO\ HOHYDWHG KHPRJORELQ YDOXHV7. Visual examination of the plasma
after centrifugation can detect the source of errors.
6DPSOHV IURP SDWLHQWV ZLWK HOHYDWHG FK\ORPLFURQV DQG WKRVH UHFHLYLQJ WRWDO SDUHQWHUDO QXWULWLRQ
DLB5
124
APPENDIX - 6
713 LQFOXGLQJ D KLJK OLSLG FRQFHQWUDWLRQ PD\ IDOVHO\ HOHYDWH WKH SODWHOHW FRXQW5,7,8.
Circulating micromegakaryocytes may be counted as white blood cells. A manual

HYDOXDWLRQ RI D EORRG VPHDU FDQ GHWHFW WKH VRXUFH RI HUURUV5.
$JJUHJDWHG SODWHOHWV PD\ IDOVHO\ HOHYDWH WKH ZKLWH EORRG FRXQW DQG SHUFHQWDJH RI
O\PSKRF\WHV 7KH LQVWUXPHQW ZLOO JLYH DQ ©/ª HUURU FRGH LI SODWHOHW DJJUHJDWHV DUH
suspected5,7.
The presence of immature white blood cells, including blasts, may affect the accuracy
of the differential5,7 7KH LQVWUXPHQW ZLOO JLYH DQ ©,ª RU ©0ª HUURU FRGH LI EODVWV DUH
VXVSHFWHG $ PDQXDO HYDOXDWLRQ RI EORRG VPHDU LV UHTXLUHG
&OLQLFDO VWXGLHV KDYH VKRZQ WKDW WKH DVVD\ SURGXFHV DFFXUDWH UHVXOWV LQ WKH SUHVHQFH
of malarial parasites, Howell-Jolly bodies, cryoglobulins and red cell fragments5,7.
BIBLIOGRAPHY :
1. Coulter WH: High speed automatic blood cell counter and cell size analyzer. Proc Natl
Elect Conf 1956;12:1034.
2. Mattern CFT, Bracket FS, Olsen BJ: Determination of number and size of particles by
electrical gating: Blood celIs. J Appl Physiol 1957;10:56.
3. International Committee for St.andardization in Haematology; Expert Petnel on

Haemoglobinometry: Recommendations for reference method for haemoglobinometry in human
EORRG ,&6+ VWDQGDUG DQG VSHFL¿FDWLRQV IRU LQWHUQDWLRQDO KDHPRJ,RELQF\XQLGH UHIHUHQFH
preparation. Clin Lab Haematol 1987; 9:73.
4. NCCLS document H3-A5, Procedure for the Collection of Diagnostic Blood Specimens by
9HQLSXQFWXUH $SSURYHG 6WDQGDUG )LIWK (GLWLRQ 9ROXPH 1R :D\QH 3$ 1&&/6
5. Watson JS, Dotson MA. Multiparameter Hematology Instruments. in Clinical Hemalology

Principles, Procedures, Correlations, Lotspeich-Steininger CA, Steine- Martin EA, Koepke JA
(ed) 1992;511
6. Bessman JD, Bamks BA Spurious macrocytosis, a common clue to coId agglutinins. Am J
Clin Pathol 1980;74;797-800.
7. Perkins SL. Examination of the Blood and Bone Marrow in Wintrobe’s Clinical
KDHPDWRORJ\ /HH *5 )RHUVW½HU - /HXNHQV - 3DUDVNHYDV ) *UHHU -3 5RGJHUV *) HG
1999;10-19.
8. /LQ] /- (OHYDWLRQ RI KHPRJORELQ 0&+ DQG 0&+& E\ SDUDSURWHLQ KRZ WR UHFRJQL]H DQG
correct the interference. Clin Lab Sci 1994; 7:211-212.
9. 1&&/6 &$ +RZ WR 'H¿QH DQG 'HWHUPLQH 5HIHUHQFH ,QWHUYDOV LQ WKH &OLQLFDO /DERUDWRU\
$SSURYHG *XLGHOLQH 6HFRQG (GLWLRQ6HFRQG (GLWLRQ 9ROXPH 1R :D\QH 3$ 1&&/6
2000.
10. NCCLS EP9-A2. (2002) Method Comparison and Bias Estimation Using Patient Samples.
$SSURYHG *XLGHOLQHV 9ROXPH 1R :D\QH 3$ 1&&/6
11. 1&&/6 (3$ (YDOXDWLRQ RI 3UHFLVLRQ 3HUIRUPDQFH RI &OLQLFDO &KHPLVWU\ 'HYLFHV 6HFRQG
Edition, Volume 19, No. 2. Wayne, PA: NCCLS; 1999.
DLB5
125
APPENDIX - 7
10.7 REAGENTS INSTRUCTIONS

Please refer to the document «Instructions for use of reagents «delivered
with the device. The reagent usage notice can also be downloaded from the
website: www.GLDODEDW
Only the 'LDODE brand Reagents must be used.
1. REAGENTS USE
Reagents are intended for haematology cell counter use (Dialab-brand kind), meaning red and white
cells, blood platelets, 3 or 5 part leukocyte differential counts.
For in vitro diagnostic use only.
2. REAGENTS CONTENT
Nature of reagents in Dialab-brand packs.
'LDOXHQW
Buffered isotonic solution - Diluent for Dialab '/% cell counter.
Content: Sodium chloride
Sodium sulphate
Stabilizer
Ammonium IV
'LDWHUJHQW
Isotonic detergent – transfer liquid for Dialab '/% cell counter.
Ethyl alcohol
Detergent
Colouring agent
'LDGLII
Lysing agent and haemoglobin assay – Intended for Dialab '/% cell counter.
Content: Ammonium IV
Potassium cyanide
Stabilizer
3UHVHUYDWLYH
'LDO\V(2
Differential lysing agent for eosinophil counting – Intended for Dialab '/% cell counter.
Potassium chloride
Borate buffer
Non-ionic surfactant
RINSE
Solution used to rinse the haematology cell counter – Used during standby.
Content: Distilled water
3UHVHUYDWLYH
DLB5
126
APPENDIX - 7
3. PRINCIPLE AND REACTION DESCRIPTION
'LDODE '/% DQDO\VHUV FRXQW HYHU\ EORRG SRSXODWLRQ FHOO E\ FHOO 5HG FHOOV :KLWH FHOOVor
blood Platelets).
Dialab-brand analysers prepare the count and the analyse by insulating blood populations at
ILUVW ZLWK D GLOXWLRQ
$ ILUVW GLOXWLRQ ZLWK 'LDOXHQW HQDEOH WKH DQDO\VH RI :KLWH FHOOV SUHVHQW LQ VPDOOTXDQWLWLHV
7KHQ D VHFRQG GLOXWLRQ LV PDGH IURP WKH ILUVW RQH WR FRXQW 5HG FHOOV DQG EORRG3ODWHOHWV PRUH
VLJQLILFDQW TXDQWLWLHV
Automatic dilutions are completed from a programmable quantity of whole blood.
7KH TXDQWLW\ RI EORRG FROOHFWHG FDQ YDU\ IURP DERXW O WR O 7KLV TXDQWLW\ YDULHV DFFRUGLQJ
to the bank used.
'LDODE '/% FHOO FRXQWHUV DUH ZRUNLQJ ZLWK WKH PHDVXUHPHQW SULQFLSOH RI D SDUWLFOH WKDW PRGLILHV
DQ HOHFWULF FXUUHQW ZKLOH SDVVLQJ WKURXJK DQ RULILFH
7KH FHOO LV VXFNHG XS IURP WKH $ WDQN XS WR WKH % WDQN E\ SDVVLQJ WKURXJK D FDOLEUDWHG RULILFH
$Q HOHFWULF FXUUHQW DSSOLHV RQ ERWK VLGHV RI WKLV RULILFH7KHFHOOGRHVQRWKDYHWKHVDPH
FRQGXFWLYLW\ WKDQ LWV HQYLURQPHQW WKHQ LWV SDVVDJH WKURXJK DQ RULILFHOHDGVWRD FKDQJHRIWKH
current established between both electrodes. This current difference is registered and increment
D FRXQWHU DW HYHU\ FHOO SDVVDJH
A chemical agent is used to separate erythrocyte and leukocyte populations, because of
size RYHUODSSLQJ DQG TXDQWLWLHV GLVFUHSDQFLHV 7KLV FKHPLFDO DJHQW FRQWDLQHG LQ O\VLV
'LDGLIIpops the cytoplasmic membrane of the Red Cells. Erythrocyte population disappears
then to let leukocytes remaining.
$ KDHPRJORELQ SUHVHUYDWLYH LV DGGHG LQ O\VLQJ DJHQW WR PHDVXUH KDHPRJORELQ VFDOHG GRZQ LQ
a 540 nm photometric tank at the end of the counting.
7KH KDHPRJORELQ PHDVXUHPHQW LV PDGH IURP WKH ILUVWGLOXWLRQ7KHO\VLQJDJHQWKDVD SRZHUIXO
haemoglobin reducer (potassium cyanide) and then the haemoglobin measurement follows
DRABKIN method with a 540 nm reading.
7KH LQWHJUDWLRQ RI OXPLQRXV LQWHQVLW\ WUDQVPLWWHG LV HYDOXDWHG DFFRUGLQJ WR WKH %((5/$0%(57
formula.
A zero reIHUHQFHSRLQWLVPDGHDIWHUHDFKDQDO\VLVZLWKVSHFLILFOLTXLG'LDOXHQW
$Q HQ]\PDWLF OLTXLG 'LDWHUJHQW HQVXUHV WKH V\VWHP FOHDQOLQHVV EHWZHHQ HYHU\ DQDO\VLV DQG
DOORZV DYRLGLQJ FRQWDPLQDWLRQ EHWZHHQ WZR FRQVHFXWLYH DQDO\VLV
4. PERFORMANCES
6SHFL¿FLW\:
6HYHUDO VXEVWDQFHV FDQ LQWHUIHUH ZLWK WRWDO FRXQW UHVXOWV

,QWHUIHULQJ VXEVWDQFHV $QDOJHVLF GHULYDWLYHV RI SURFDLQH HVWHU
- Medication with anticoagulant actions:
2UDO DQWLFRDJXODQW . DQWLYLWDPLQ ,,, DQG ,9 $QWLWKURPELQ
3DWLHQWV WUHDWPHQW ZLWK &DOFLXP KHSDULQDWH GHULYDWLYHV IURP
Choay laboratory, for concentration exceeding 4-6 mg/j
+LJK YROXPH FRUWLVRQH WUHDWPHQW
The case of lipemic serum is different, but can also affect results.
DLB5
127
APPENDIX - 7
Method limits:
- Samples that can be used for a 5 part-diff :

Whole blood collected with anticoagulant K3 EDTA in plastic or glass tube.
,WLVQHFHVVDU\WRFROOHFWEORRGRQDUDGLDOYHLQDMXJXODUYHLQRUDQ\RWKHUYHLQRIVXI¿FLHQWGLDPHWHUWR
DOORZDTXLFNFROOHFWLRQ5RXJKEORRGDVSLUDWLRQLVWREHDYRLGHGEHFDXVHRIKDHPRO\VLVSKHQRPHQRQ
LWFDQFDXVH&ROOHFWLRQWLPHDOVRKDYHWREHOLPLWHGWRDYRLGPLFURFRDJXODWLRQSUREOHPV
$ORQJDQGVPRRWKKRPRJHQL]DWLRQRIEORRGDQGDQWLFRDJXODQWPL[LVHVVHQWLDOEHIRUHHYHU\DQDO\VLV
- 6DPSOHSUHVHUYDWLRQ :
Whole blood samples are stabile during about 4 hours at room temperature and 24 hours between 2
and 6°C.
- Analysis :
During the analysis, other factors can interfere and modify results.
Room temperature and airing within the workspace are really important for samples as well as for
reagents and can affect results. Then, it is recommended not to place the analysers behind a window,
HVSHFLDOO\LILWLVVXQQ\$FORVHGDQGZHOOYHQWLODWHGURRPLVDGYLVHG
2WKHU IDFWRUV VXFK DV HOHFWURPDJQHWLF UDGLDWLRQ RU HOHFWULF QHWZRUN FKDUDFWHULVWLF DOVR KDYH WR EH
considered.
,W LV KLJKO\ UHFRPPHQGHG QRW WR SODFH WKH DQDO\VHU QH[W WR DQRWKHU HOHFWULF GHYLFH HPLWWLQJ KLJK
electromagnetic radiation that could interfere with analysis results.
Besides, it is recommended to test the earth electrode of the analyser plug, because a default could
bring erroneous results.
5. RESULTS
Measured Parameters:
White blood cell in thousands/mm3
Red blood cell in millions/mm3
Platelet in thousands/mm3
Hematocrit in %
Hemoglobin in g/l
Eosinophils in thousands/mm3
Calculated parameters:
0&9 0HDQ &RUSXVFXODU 9ROXPH +&75%& [ LQ À
MCH: Mean Corpuscular Haemoglobin (Hb/RBCx10) in pg
MCHC: Mean Corpuscular Haemoglobin Concentration (Hb/HCTx100) in g/dl
/\PSKRF\WHV LQ DEVROXWH YDOXH LQ WKRXVDQGVPP3
Lymphocytes in %
0RQRF\WHV LQ DEVROXWH YDOXH LQ WKRXVDQGVPP3
Monocytes in %
*UDQXORF\WHV LQ DEVROXWH YDOXH LQ WKRXVDQGVPP3
Granulocytes in %
1HXWURSKLOV LQ DEVROXWH YDOXH LQ WKRXVDQGVPP3
Neutrophils in %
Eosinophils in %
Basophils in %
Supply parameters:
PCT: Plateletcrit in %
039 0HDQ 3ODWHOHW 9ROXPH LQ À
PDW: Platelet Distribution Width without units
RDW: Red cells Distribution Width without units
DLB5
128
APPENDIX - 7
6. PRESERVATION NOTES:
Reagents must be stored at room temperature between 15°C.... 25°C (59° and 77°F).
6LJQL¿FDQW WHPSHUDWXUH YDULDWLRQ DUH WR EH DYRLGHG
:KLOH FORVHG UHDJHQWV KDYH DQ PRQWKVORQJ H[SLU\ GDWH or more for some (refer to expiry
dates shown on the labeling).
When opened, reagents can be used within 16 weeks.
7. GUIDELINE:
See Reagents Installation section 2.1.3 and Reagents Replacement section 8.2 of this
manual.
8. SPECIAL SAFE PRACTICE:
Respect safety instructions and expiry dates.

For in vitro diagnostic use only.
Refer to the Instructions for use of reagents.
DLB5
129
APPENDIX - 8
10.8 ERROR MESSAGES

(Sorted by type)
Press a key to acknowledge an error message
TYPE MESSAGE SIGNIFICATION SOLUTION
POST $PSOL¿HUERDUGQRW2. 1RDQVZHUIURPDPSOL¿HUERDUG &KHFNÀDWFDEOHEHWZHHQDPSOL¿HU

board and mother board
POST Carrier not OK 3%PRWRU(QGRIVWURNHVZLWFKÀDW Gear loose / Verify switches

cable / Tubes blocked / Mother board
POST Counting chip fault. PB PowerCell Replace Mother board
POST Management system fault PB back up components group Replace Mother board
POST Needle not OK PB motor / Mother board / Switch Gear loose / Verify switches
POST Pressure sensor or Atmospheric Zero pressure uncalibrated / Air leak Verify Air circuit / Replace Mother
pressure not OK board
POST Pressure sensor Sensor broken / Air leak Verify Air circuit / Replace Mother
unplugged or Air leakage board
POST Photometer not OK PB LED / Photo transistor / cables / 9HULI\FDEOHVIURPDPSOL¿HUERDUG

Mother board Replace Photometer / Mother board
POST Safeguard system fault PB (Management) Safeguard Replace Mother board

component
POST SAFEGUARD LOST PB RTC Battery / Main power surge / (Replace RTC battery)
ALL DATA Upgrade program
POST SafeKeeping lost PB RTC Battery / Main power surge (Replace RTC battery)
all Analyses!
POST SafeKeeping lost PB RTC Battery / Main power surge (Replace RTC battery)
Quality control test
POST SafeKeeping Err. PB RTC Battery / Main power surge (Replace RTC battery)
System Setup lost. 9HULI\V\VWHPFRQ¿JXUDWLRQ
Control Blood Parameters Enter parameters
Blood Parameters Enter parameters
POST Syringe not OK PB motor / Mother board / Switch 0RYHSLVWRQPDQXDOO\9HULI\VZLWFK
POST Temperature probe Temperature sensor malfunction Replace Mother board temperature
not OK sensor
POST Power Fail External Power Supply malfunction Replace external power supply /
+24V circuit (too low or too high) Connection board
POST Power Fail Internal Power Supply malfunction or Replace internal power supply /
+12V / -12V circuit +/-12V short circuit Mother board
POST : POWER ON SELF TEST

PB : Problem
DLB5
130
APPENDIX - 8
10.8 ERROR MESSAGES

(Sorted by type)
CTRLQ ACTION DENIED Identify from MENU - PARAMETER

Control blood QUALITY CT
QRWLGHQWL¿HG
CTRLQ ACTION DENIED 5HSODFH%ORRG,GHQWL¿FDWLRQ
Control blood
out of date.
HARD PHOTOMETER ERROR PB Calibration / Measuring Chamber Close Chamber / Replace LED /
w/o hood / Photo transistor Photo transistor
HARD ILLEGAL HARDWARE N Contact Distributor

SOFT Divide by 0 Contact Distributor
SOFT Escape Code INT Contact Distributor
SOFT INT nn Contact Distributor
SOFT NMI Interrupt Contact Distributor
SOFT 2YHUÀRZ,17 Contact Distributor
SOFT Unused OPCODE Contact Distributor
CTRLQ : QUALITY CONTROL

HARD : PB HARDWARE
SOFT : PB SOFTWARE
PB : Problem
DLB5
131
APPENDIX - 8
10.8 ERROR MESSAGES

(Sorted by type)
ACTION DENIED Following POST Error

NORM $PSOL¿HUERDUG 6HH3267$PSOL¿HU(UURU
in error
ACTION DENIED Following POST Error
NORM Motor error See POST Motor Error
ACTION DENIED
Pressure sensor Following POST Error See POST Pressure sensor Error
NORM Out of order
ACTION DENIED You try to go in Standby mode but

NORM System already in the instrument is already in Standby
standby mode. mode
ANALYSIS REJECT
NORM No interpretation RBC quantity is too small (> 0.5 M) Try Analysis again
possibility
ANOMALY
NORM Air leakage Air circuit leak 9HULI\WXEHV9DOYH
Check tubes
ANOMALY
NORM DETERGENT empty No Reagent / PB Sensor / Leak 9HULI\WXEHV9DOYHVVHQVRU
Check levels
ANOMALY
NORM DETERGENT empty or Detergent missing or Air circuit leak 9HULI\WXEHV9DOYHV
Air leakage
ANOMALY
NORM DILUENT empty No Reagent / PB Sensor / Leak 9HULI\WXEHV9DOYHVVHQVRU
Check levels
ANOMALY
NORM EO-DIFF reagent empty No Reagent (EO Lyse) / PB Sensor 9HULI\WXEHV9DOYHVVHQVRU
Check levels / Leak
ANOMALY PB Transfer EO Dilution or 7XEHVEORFNHG&ORW¿OWHUVEORFNHG

NORM Dilution transfer Predilution -> measuring chamber 9DOYHV
ANOMALY
No Reagent (3PD Lyse) / PB Sensor
NORM LYSE empty 9HULI\WXEHV9DOYHVVHQVRU
Check levels / Leak
PB Parameters Hardware Menu

ANOMALY
(Vacuum), PB Dilution qty, PB Air 9HULI\3DUDPHWHUV9DOYH
NORM Vacuum mismatch: xxx
circuit leak, PB Transfer
NORM : Normal use

PB : Problem
DLB5
132
APPENDIX - 8
10.8 ERROR MESSAGES

(Sorted by type)
NORM ERROR PB Blank Reference for Hb See photometer error

Photometer Calibration
NORM ERROR PB End of stroke / location switch or Verify switch / Unblock tubes
Translation carrier
tubes blocked
NORM ERROR PB End of stroke switch / Motor Verify switch / Unblock motor
Up/Down Needle
blocked
NORM MS-CARD ERROR PB SmartCard Replace SmartCard
Illegal MS-CARD into the
Card Reader
NORM MS-CARD ERROR MS-CARD has not be replaced with Insert a new SmartCard
MS-CARD EMPTY
new reagent Pack
Insert new card before
next analysis
NORM MS-CARD ERROR PB SmartCard / PB card reader Replace SmartCard / Mother board
Test fault while MS-CARD
control. Insert new one
NORM MS-CARD ERROR Wrong side / PB Card / PB card Replace SmartCard / Mother board
Unknown or bad inserted
reader
MS-CARD into the Card
Reader
NORM NOT MODIFIABLE This parameter cannot be changed

NORM Pos. Carrier ERR PB Location switch or tubes blocked Verify switch / Unblock motor
NORM Priming DETERGENT error PB Sensor / Leak / Detergent empty 9HULI\'HWHUJHQWOHYHO7XEHV
Check Levels 9DOYHVVHQVRU
NORM Priming DILUENT and PB Sensor / Leak / Diluent or Lyse 9HULI\'LOXHQWDQG/\VHOHYHOV

LYSE error 7XEHV9DOYHVVHQVRU
empty
Check Levels
NORM Priming DILUENT error PB Sensor / Leak / Diluent empty 9HULI\'LOXHQWOHYHO7XEHV9DOYHV

Check Levels / sensor
NORM Priming LYSE error PB Sensor / Leak / 3PD Lyse empty 9HULI\3'/\VHOHYHO7XEHV
Check Levels 9DOYHVVHQVRU
NORM Priming Eosino reagent PB Sensor / Leak / EO Lyse empty 9HULI\(2/\VHOHYHO7XEHV9DOYHV

error / sensor
Check Levels
NORM : Normal use

PB : Problem
DLB5
133
APPENDIX - 8
10.8 ERROR MESSAGES

(Sorted by type)
Priming RINSE SOLUTION PB Leak / Rinse solution empty

NORM error 9HULI\5LQVH6ROXWLRQOHYHO7XEHV
9DOYHV
Check Levels
PRINTING External Printer power off / out of
NORM Ext. Printer not available. paper... 9HULI\H[WHUQDOSULQWHUYDOLGDWHWR
print on internal printer or cancel
Printing on internal
Printer
PRINTING 1RSDSHULQLQWHUQDOSULQWHURUFRYHU
NORM Paper empty or 9HULI\SDSHUSXVKWKHFRYHUGRZQ
bad closed
if paper present
cover open
TRANSMIT RESULTS Occurs when trying to export results Verify cable between DLB5 ® and
NORM SERIAL PORT #2 to computer FRPSXWHU&RPSXWHUPXVWKDYHD
OFF LINE program RUNNING to open its Port
Occurs during cleaning cycle after Verify carrier or reagents
NORM Problem during rinsing analysis
SAMPLING ERROR Cap on blood tube 5HPRYHWKHFDSRQEORRGWXEHDQG
NORM Cap on blood tube try again
MS-CARD BANK 3%WRVDYH%ORRG%DQNRQD Replace SmartCard / Mother board
NORM WRITE ERROR BLOOD BANK CARD
MS-CARD Blood Bank name different between If ‘Continue’ is selected, data will be
NORM The DLB5 Bank does not DLB5 ® memory and MS-CARD lost
match the MS-CARD Bank
)RUDQ\XQVROYHGSUREOHPSOHDVHFRQWDFWWKH6$9RU\RXUGLVWULEXWRU
'LDODE UHFRPPHQGV WR UHTXHVW WKH ODWHVW XSWRGDWH YHUVLRQ RIthe

analyzer software
DLB5
134
APPENDIX - 9
Dialab
‘SOFTWARE & MS-Card’ LICENSE AGREEMENT
IMPORTANT - READ BEFORE INSTALLING OR USING

This document is a LEGAL AGREEMENT between you and Dialab. Do not use or load any of your new
in vitro diagnostic Dialab Instrument’s Software and/or any associated materials as MS-Card (the “reagent
card”) until you have carefully read the following terms and conditions. By loading and/or using the Software
and/or using the MS-Card used with your new Dialab instrument, you agree to be bound to the terms of
this License Agreement. If you do not wish to so agree, DO NOT install or use the Software or any other
associated materials as MS-Card.
MANUFACTURER 7KH ³0DQXIDFWXUHU´ ZRUG XVHG KHUHLQ LQFOXGHV L FRPSDQLHV KDYLQJ RULJLQDOO\ FUHDWHG \RXU DQDO\]HU¶V 6RIWZDUH
DQGRU DQ\ DVVRFLDWHG PDWHULDOV DV WKH 06&DUG HOHFWURQLF FDUG ZKLFK VXSHUYLVHV WKH UHDJHQW TXDOLW\ DQG LQFOXGHV RZQHU¶V FRGHV
(ii) companies licensed to manage any rights and/or copyrights linked to any Intellectual property used in the machine and/or in
DQ\ DVVRFLDWHG PDWHULDO DV WKH 06&DUG LLL LQGLYLGXDO ZKR KDV DQ\ ULJKWV RQ DQ\ RI WKH ,QWHOOHFWXDO SURSHUW\ XVHG LQ WKH PDFKLQH
and/or in any associated material as the MS-Card,
Dialab INSTRUMENTS. The “Dialab Instruments” words used herein include Instrument(s), (i) manufactured by Dialab - Austria and/
RU LWV DI¿OLDWHV DQGRU VXEVLGLDULHV DQG GLVWULEXWHG GLUHFWO\ RU LQGLUHFWO\ E\ PHDQ RI ORFDO 'LVWULEXWRUV LL PDQXIDFWXUHG E\ 'LDODE
$XVWULD DQGRU LWV DI¿OLDWHV DQGRU VXEVLGLDULHV IRU RWKHU FRPSDQLHV XQGHU DQ 2(0 DJUHHPHQW
LICENSE. You may copy and/or install any Manufacturer’s Software onto the single built-in computer (or computer board) of your
new Dialab instrument for your personal, noncommercial use, and you may NOT make any backup copy of the Software. You may
XVH WKH 06&DUG RQ \RXU 'LDODE LQVWUXPHQW WR UXQ LW DQG SUR¿W RI LWV UHDJHQW TXDOLW\ PDQDJHPHQW %87 \RX PD\ 127 PDNH DQ\
backup and/or copy and/or duplicate and/or compatible material of this card.
OWNERSHIP OF SOFTWARE AND COPYRIGHTS. Title to all copies of the Software remains with the Manufacturer or its suppliers.
7KH 6RIWZDUH LV FRS\ULJKWHG DQG SURWHFWHG E\ WKH (XURSHDQ ODZV DQG RWKHU FRXQWULHV DQG LQWHUQDWLRQDO WUHDW\ SURYLVLRQV <RX
PD\ QRW UHPRYH DQ\ FRS\ULJKW QRWLFHV IURP WKH 6RIWZDUH 7KH 0DQXIDFWXUHU PD\ PDNH FKDQJHV WR WKH 6RIWZDUH DQGRU WR LWHPV
referenced therein as the MS-Card, at any time without notice, but is not obligated to support or update the Software. Except as
RWKHUZLVH H[SUHVVO\ SURYLGHG WKH 0DQXIDFWXUHU JUDQWV QR H[SUHVV RU LPSOLHG ULJKW XQGHU LWV SDWHQWV FRS\ULJKWV WUDGHPDUNV RU RWKHU
intellectual property rights. You may transfer the Software and/or any associated materials as MS-Card only if the recipient agrees
to be fully bound by these terms and if you retain no copies of the Software.
LIMITED MEDIA WARRANTY ,I WKH 6RIWZDUH KDV EHHQ GHOLYHUHG GLUHFWO\ E\ WKH 0DQXIDFWXUHU RU RQH RI LWV 'LVWULEXWRUV RQ
physical media, the Manufacturer warrants the media to be free from material physical defects for a period of ninety (90) days after
GHOLYHU\ E\ WKH 0DQXIDFWXUHU RU RQH RI LWV 'LVWULEXWRUV ,I VXFK D GHIHFW LV IRXQG UHWXUQ WKH PHGLD WR WKH 0DQXIDFWXUHU RU \RXU
ORFDO 'LVWULEXWRU IRU UHSODFHPHQW RU DOWHUQDWH GHOLYHU\ RI WKH 6RIWZDUH DV WKH 0DQXIDFWXUHU PD\ VHOHFW 7KH OLPLWHG PHGLD ZDUUDQW\
is the same for the MS-Card if the MS-Card has not been already in use.
EXCLUSION OF OTHER WARRANTIES EXCEPT AS PROVIDED ABOVE, THE SOFTWARE IS PROVIDED “AS IS” WITHOUT
ANY EXPRESS OR IMPLIED WARRANTY OF ANY KIND INCLUDING WARRANTIES OF MERCHANTABILITY, NONINFRINGEMENT,
OR FITNESS FOR A PARTICULAR PURPOSE. The Manufacturer does not warrant or assume responsibility for the accuracy or
completeness of any information, text, graphics, links or other items contained within the Software and/or the MS-Card.
MANUFACTURER LIMITATION OF LIABILITY. IN NO EVENT SHALL THE MANUFACTURER OR ITS SUPPLIERS BE LIABLE
FOR ANY DAMAGES WHATSOEVER (INCLUDING, WITHOUT LIMITATION, LOST PROFITS, BUSINESS INTERRUPTION, OR LOST
INFORMATION) ARISING OUT OF THE USE OF OR INABILITY TO USE THE SOFTWARE, EVEN IF THE MANUFACTURER
OR ITS SUPPLIERS HAVE BEEN ADVISED OF THE POSSIBILITY OF SUCH DAMAGES. SOME JURISDICTIONS PROHIBIT
EXCLUSION OR LIMITATION OF LIABILITY FOR IMPLIED WARRANTIES OR CONSEQUENTIAL OR INCIDENTAL DAMAGES,
SO THE ABOVE LIMITATION MAY NOT APPLY TO YOU. YOU MAY ALSO HAVE OTHER LEGAL RIGHTS THAT VARY FROM
JURISDICTION TO JURISDICTION.
USER LIABILITY. IN THE EVENT YOU DO NOT RESPECT DIRECTLY OR INDIRECTLY, AND/OR, INFRINGES PARTIALLY OR
ENTIRELY THE MANUFACTURER’S INDUSTRIAL PROPERTY -AND/OR ANY OTHER COMPANY’S INDUSTRIAL PROPERTY
USED BY ANY OF DIALAB INSTRUMENTS- INCLUDING SOFTWARE COPYRIGHT, PATENTS, TRADE MARKS, LOGO, MS-
CARDS TECHNOLOGY OR OTHERS, OR, HELP OR PARTICIPATES WITH A THIRD PARTY NOT TO RESPECT THE SAME
MANUFACTURER’S INDUSTRIAL PROPERTIES -AND/OR ANY OTHER COMPANY’S INDUSTRIAL PROPERTY USED BY ANY
OF DIALAB INSTRUMENTS- AS ABOVE MENTIONED, INCLUDING DISASSEMBLING THE SOFTWARE OF ANY OF DIALAB
INSTRUMENTS AND/OR ANY KIND OF OTHER REVERSE-ENGINEERING ACTION, YOU ARE ENTIRELY LIABLE TO THE
MANUFACTURER FOR ANY SPECIAL DAMAGES, INCLUDING LOST OF PROFITS, LOST SAVINGS OR OTHER INCIDENTAL
OR CONSEQUENTIAL DAMAGES OF ANY KIND. ANY FAILURE TO RESPECT THE MANUFACTURER’S INDUSTRIAL
PROPERTY SHALL BE DEEMED AS A CONTRACT VIOLATION AND A MATERIAL BREACH OF THIS AGREEMENT.
APPLICABLE LAWS &ODLPV DULVLQJ XQGHU WKLV $JUHHPHQW VKDOO EH JRYHUQHG E\ WKH ODZV RI \RXU FRXQWU\ DQGRU FRXQWULHV RI
(XURSH H[FOXGLQJ LWV SULQFLSOHV RI FRQÀLFW RI ODZV DQG WKH 8QLWHG 1DWLRQV &RQYHQWLRQ RQ &RQWUDFWV IRU WKH 6DOH RI *RRGV <RX
PD\ QRW H[SRUW WKH 6RIWZDUH DQGRU DQ\ DVVRFLDWHG PDWHULDOV DV 06&DUGV LQ YLRODWLRQ RI DSSOLFDEOH H[SRUW ODZV DQG UHJXODWLRQV
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Operator's Manual: Automated

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AUTOMATED

Operator’s Manual HUMAN: 2019-02

Indication for Use :

1. WARNINGS AND CAUTIONS .........................................11

2. INSTALLATION PRESENTATION ................................ 17

3. FIRST UTILIZATION ............................................................. 29

3.3 RUN AN ANALYSIS ............................................................... 35

5 QUALITY CONTROL ........................................................... 65

9 EXPORT DATA ......................................................................... 99

10 APPENDIX ................................................................................. 113

PLEASE KEEP AVAILABLE THIS ‘OPERATOR MANUAL’ FOR ANY FUTURE

This instrument is marked with a

Obligation in vitro diagnostic medical de-

Read instruction before use

Warning / Risk of hurt

The electrical and electronic

1.1. GENERAL INFORMATION

Only properly trained personnal shall use instrument.

1.3. INSTALLATION AND UTILIZATION

1.4.1. AGAINST ELECTRICAL HAZARD

1.4.2 AGAINST BIOHAZARD

Biological samples are potentially hazardous biological materials.

Follow good laboratory practices when handling these products.

1.5. REAGENTS HANDLING

SHARP OR ANGULAR COMPONENTS HAZARD

1.7 DISPOSAL OF WASTES AND DEVICE

2.1.1 Check equipment

One reagent tube kit

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2.1.2 Instrument preparation

3) Access inside of DLB5 . 4) Open the DLB5 .

5) Steadying the hood. 6) Open position.

 3XOORIIWKHVHOIDGKHVLYHWDSHGXVW 8) Close the machine.

When running, never

2) Connection of tubes to Reagent Pack

The UHDJHQWV must be kept at the same

2) Choose the correct tube from the tube kit.

Connection of tubes to the intrument

On the lower left side of the instrument you

Insert the reagent tubes contained in the

The colours between the tubes

Black plug for the waste is positioned

- Yellow : 'LDGLII (pale yellow liquid) - Orange : 'LDO\V(2 (colorless liquid)

2.1.4 Electrical connections

Serial port LED connexions status

Parallel and USB printer ports

CONNECT IEC 60950-1 AND IEC 61010-1 COMPLIANT PERIPHERALS ONLY

3/5 part-diff key

DLB5 keyboard use

2.2.2 External alphanumerical keyboard use

DLB5 front panel External keyboard

Escape Key Escape key

2.3 SETTING UP ADVICE

A working environment between 20°C... 25°C (68°F... 77°F) is required to stay in

DLB5 complies with emission and immunity requirements of EN 61326-2- 6.

2.3.2 Power connection

1- External power supply 1

2- Internal power supply

Input: 24.0 V 6.25A

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3XOORIIWKHVHOIDGKHVLYHWDSHGXVW 8) Close the machine.

- Yellow : 'LDGLII (pale yellow liquid) - Orange : 'LDO\V(2 (colorless liquid)

Ref rolls : +

7RFKDQJHIURPRQH¿HOGWRDQRWKHU Right and Left arrows

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,IWKH:%&UHVXOWLVYHU\KLJKXSSHUWKDQPPP3), the DLB5 displays the following warning

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