Operating Manual Cyto-Tek 2500 Cytocentrifuge (GB)

Cyto-Tek 2500
Cytocentrifuge
a t i n g
p er
O nual
M a
ating
Cyto-Tek 2500 ®
Oper al
Cytocentrifuge Manu
© 2014 Sakura Finetek USA, Inc.
All Rights Reserved

Printed in U.S.A.
Manufactured for:
Sakura Finetek USA, Inc., Torrance, CA 90501 U.S.A.
Sakura Finetek Japan Co., Ltd., Tokyo, 103-0023, Japan
Sakura Finetek Europe B.V., 2408 AV Alphen aan den Rijn, NL
0007129-01 Rev.A
TABLE OF CONTENTS
1. INTRODUCTION
1.1 Intended Use .......................................................................................................................................................................... 3
1.2 General Description ............................................................................................................................................................... 3
1.3 Physical Characteristics ......................................................................................................................................................... 3
1.3.1 Components and Figures .............................................................................................................................................. 3
1.3.2 Guard Bowl Unit .............................................................................................................................................................. 4
1.3.3 Control Panel .................................................................................................................................................................. 5
1.4 Specifications ......................................................................................................................................................................... 6
1.5 Safety Standards.................................................................................................................................................................... 6
2. INSTALLATION AND SET UP

2.1 General Information ............................................................................................................................................................... 9
2.2 Installation Requirements ....................................................................................................................................................... 9
2.3 Unpacking .............................................................................................................................................................................. 9
2.4 Unpacking the Instrument ...................................................................................................................................................... 9
2.5 Standard Accessories and Options ...................................................................................................................................... 12
2.5.1 Standard Accessories ................................................................................................................................................... 12
2.5.2 Options/Consumables................................................................................................................................................... 12
2.5.3 Missing or Damaged Items? ......................................................................................................................................... 12
3. OPERATING INSTRUCTIONS
3.1 Operating Instructions .......................................................................................................................................................... 13
3.1.1 Workflow (Figure 3A) .................................................................................................................................................... 13
3.2 Customization of Settings..................................................................................................................................................... 13
3.2.1 Creating a Program....................................................................................................................................................... 13
3.3 Assembling the Specimen Chamber and Dispensing the Specimen.................................................................................... 16
3.4 How to Load the Rotor ......................................................................................................................................................... 18
3.5 Starting the Operation .......................................................................................................................................................... 20
3.6 Discontinuing the Operation ................................................................................................................................................. 20
3.7 Emergency Stop................................................................................................................................................................... 20
3.8 Removing the Guard Bowl ................................................................................................................................................... 21
4. CARE OF THE INSTRUMENT

4.1 General Information ............................................................................................................................................................. 23
4.1.1 Cleaning the Control Panel ........................................................................................................................................... 23
Page - 1 -, Revised 31 March 2014

4.1.2 Cleaning the Instrument ................................................................................................................................................ 23
4.1.3 Disassembling and Cleaning the Guard Bowl Unit ....................................................................................................... 23
4.1.4 Cleaning 6mL/12mL specimen chambers, 6mL/12mL rubber gaskets, 6mL/12mL chamber cap and chamber holder 26
4.1.5 Gasket Replacement .................................................................................................................................................... 27
4.1.6 Preventive Maintenance by a Service Contractor ......................................................................................................... 27
5. TROUBLESHOOTING AND SERVICE

5.1 Power Outage ...................................................................................................................................................................... 29
5.2 Actions to be Taken When an Error Occurs ......................................................................................................................... 29
5.3 Troubleshooting Charts ........................................................................................................................................................ 30
5.3.1 Error Code of Monitored Errors..................................................................................................................................... 31
5.3.2 Other Errors .................................................................................................................................................................. 33
5.4 Service Information .............................................................................................................................................................. 34
5.4.1 When there is a Problem with the Instrument ............................................................................................................... 34
®
5.4.2 Cyto-Tek Centrifuge PreService Checklist ................................................................................................................. 34
5.4 Accessory Items ................................................................................................................................................................... 35
5.5 Replacement Parts............................................................................................................................................................... 35
5.6 Where to Order .................................................................................................................................................................... 35
6. SAFETY PRECAUTIONS
6.1 Safety Precautions ............................................................................................................................................................... 37
6.1.1 Dangers ........................................................................................................................................................................ 38
6.1.2 Warnings....................................................................................................................................................................... 39
6.1.3 Cautions........................................................................................................................................................................ 39
Page - 2 -, Revised 31 March 2014

1. INTRODUCTION
1.1 Intended Use 1.3 Physical Characteristics
1.3.1 Components and Figures
®
The Cyto-Tek 2500 Centrifuge is designed for the
purpose of transferring cells onto a glass slide from
cytological specimens contained in a specimen chamber
(Figure 1B)
through the process of centrifugation. The instrument, as The components of the Cyto-Tek 2500 Centrifuge are
part of the cytology process, is intended to facilitate the shown and described in the following figures and
in vitro examination of human and animal cells for paragraphs. Read and review them carefully. Locate and
morphology changes by a cytopathologist. identify each component of this instrument before
attempting to install, program, and operate it.
⑤
1.2 General Description ④
③
The Cyto-Tek 2500 Centrifuge (Figure 1A) is a self
contained instrument designed to transfer cells from
suspension onto a glass slide by cytocentrifugation. This
instrument has the capacity for up to 12 specimen holders.
Each specimen holder will accommodate a specimen of
up to 12 mL. A mono-layer of cells from the specimen are
deposited on a glass slide in a specific, square area;
therefore, screening of the slide is simplified. ②
This instrument ensures specimen integrity through ①
automatic, paced controlled optimal acceleration. The
rotor speed is programmable in 10-rpm increments from
200 rpm to 2500 rpm. Centrifugation times are
programmable from 1 second to 99 minutes 59 seconds.
The software can store up to 30 programs.
The ON-OFF switch, control panel, and digital displays are
all located on the front of the instrument for easy access.
The Cyto-Tek 2500 was designed with enhanced features ⑦
focusing on user safety, including a new sealable rotor ⑥
housing with lid which significantly reduces aerosol
exposure. The rotor housing is completely removable and
transportable, which enable loading and unloading
specimens away from the centrifuge. The Cyto-Tek 2500
Centrifuge is designed for years of reliable, efficient,
smooth, and quiet operation.
⑧
Figure 1A
Figure 1B
Page 3, Revised 31 March 2014

1. INTRODUCTION
① Power Switch ⑦
Turns the instrument power on and off.
② Control Panel
Provides the control buttons and indicators to display the
instrument status.
③ Instrument Lid
Is opened and closed when the guard bowl unit is placed into the
instrument. A lid interlock mechanism is provided to prevent ⑤
access to the guard bowl unit during operation. Also provided are
⑥ Figure 1D
the lid sensor for detecting that the lid is closed and the lid lock
sensor for detecting that the lid is locked properly. The lid has a
lid gasket to prevent leakage from the guard bowl. ① Guard Bowl
Accommodates the rotor. The guard bowl is detachable and has
④ Transparent Window its own detachable bowl lid.
Is provided in the instrument lid to allow the operator to view the The bowl lid can reduce aerosols and splashes from specimen
rotation status of the guard bowl. chambers in the rotor during centrifugation.
⑤ Lid Open Push Button The rotor is removed from the guard bowl by loosening two
Allows the instrument lid to lift up. By gently pressing the button thumbscrews that hold the rotor in place. Splashes from specimen
located in front of the transparent window, the lid is lifted up and chambers during centrifugation are accumulated inside the guard
then opened by operator to the open position. To close the lid, bowl. The guard bowl must be drained after every run.
lower the lid toward the front and push down. ② Rotor
⑥ Emergency Lid Release Opening Has compartments numbered 1 to 12 as an aid for chamber
⑦ Power Cord Connector (with fuse holders) management. The rotor fastened to the guard bowl serves as a
stand when fluid samples are dispensed into specimen chambers.
Accepts the instrument end of the power cord.
③ Bowl Shaft Housing
⑧ Lid Gasket
④ V-shape Gasket
⑨ Spindle
Connects to the bowl shaft housing of the guard bowl to transfer ⑤ Bowl Lid
rotation from the motor. Securely seals the guard bowl. The lid lock button is provided on
top of the lid knob. The bowl lid is locked by pushing down the
1.3.2 Guard Bowl Unit (Figure 1C & 1D) button and can be opened by pulling it up (Figure 1E).
The guard bowl unit is comprised of the guard bowl, rotor,

and bowl lid.
The bowl lid must be put on the guard bowl during
handling and operation.
CAUTION: The guard bowl and bowl lid are adjusted Locked
unit by unit. Avoid using a combination of the guard
bowl with the bowl lid of a different unit. Lock button
④ ②
③ Lid knob
Unlocked
Figure 1E
⑥ Bowl Lid Gasket
⑦ Part Number of the Guard Bowl
①
Figure 1C

1.3.3 Control Panel (Figure 1F)
⑤ ⑥ ⑦
① Figure 1F
⑨ ⑧ ② ③ ④
① START/STOP key
Starts and stops the operation.
② SET key
Switches the Standby mode to the Program mode and the
Set & View mode.
③ (down-arrow) key
Changes a program number and decreases a setting value
for the rotation speed or process time.
④ (up-arrow) key
Changes a program number and increases a setting value
for the rotation speed or process time.
⑤ Program Number display (7-segment display)

Displays a program number from 1 to 30.
⑥ Speed display (7-segment display)

Displays a programmed rotation speed or an actual speed.
⑦ Time display (7-segment display)

Displays a programmed process time or a process time left.
⑧ Lid Lock indicator

An orange light is displayed when the instrument lid is
locked prior to the start of operation and goes out when the
lid is unlocked after the motor has stopped.
⑨ Power indicator
Lights up while the power is supplied to the instrument.

1. INTRODUCTION
1.4 Specifications Operating Conditions:

Operational temperature - 10 to 40°C (50 to 104°F)
Power Required: Operational relative humidity - 30 to 85%RH
Power Rating - AC100 to 240V, 50/60Hz (non-condensing)
Power Consumption - 100VA Storage temperature - –20 to 60°C (–4 to 140°F)
Power protection - Class I, Pollution Degree 2 Storage relative humidity - 20-90% RH
(non-condensing)
Installation Category: Noise level - 50 dBA or lower
(when properly balanced)
Class II
Instrument Life Expectancy:
Weight Main Body:
7 Years.
10 Kg (22 lbs.)
Conditions: The safety precautions provided in the
Weight: Guard Bowl operating manual and medical package insert must be
1.7Kg (3.7lbs) observed. The instrument needs to be regularly
maintained according to the instructions in Section 4, Care
Overall Dimensions: of the Instrument.
22.5 cm (8.9 in) high (with lid closed) NOTE: It is recommended that preventative maintenance
is performed on the instrument once a year.
59.5 cm (23.4 in) high (with lid opened)
49.0 cm (19.3 in) deep
37.5 cm (14.8 in) wide
1.5 Safety Standards
Holder Capacity: Complies with: UL 61010-1, 2nd:2004
12 Specimen Holders UL61010-2-020:2006
1mL / 6mL / 12mL specimen chambers CAN/CSA C22.2 No. 61010-1-04
CAN/CSA C22.2 No. 61010-2-20-09
Glass Slide Sizes - Length: 76 +0/-1mm CAN/CSA C22.2 No. 61010-2-101
Width: 26 +0/-1mm IEC 61010-2-101
Thickness: 1.1 +0.1/-0.2mm
Safety Features:
Lid lock, Guard bowl monitor (imbalanced rotation
Rotor Operating Speeds: detector), Over-current protection, Over-heat protection
From 200 rpm to 2500 rpm; in 10 rpm increments
Programmable Time Settings:

Program memory-up to 30
From 1 second to 99 minutes 59 seconds; selectable in
minutes and seconds

CAUTION: The instrument is intended to be operated
indoors. Do not use it outdoors.
COMPARISION OF ROTATION SPEED RELATIVE TO

CENTRIFUGAL FORCE
Relative
Acceleration
Rotation speed Centrifugal
(m/s2)
(rpm, min-1) Force (g)
Approx.
Approx.
200 5 46
300 11 104
400 19 184
500 29 288
600 42 414
700 58 564
800 75 737
900 95 932
1000 117 1151
1100 142 1393
1200 169 1658
1300 198 1946
1400 230 2256
1500 264 2590
1600 301 2947
1700 339 3327
1800 380 3730
1900 424 4156
2000 470 4605
2100 518 5077
2200 568 5572
2300 621 6090
2400 676 6631
2500 734 7195
NOTES:
・ The distance from the center of the rotor to a specimen
chamber installed is 105 mm.
・ Relative centrifugal force (RCF) = 11.739 x 10-5 x N2 (g)
・ N=Revolutions per minute (rpm)

1. INTRODUCTION

2. INSTALLATION AND SETUP
Accessories packed in the secondary accessory carton
2.1 General Information include:
This section provides information on selecting a proper Check
Description Quantity
location, unpacking, and installing the Cyto-Tek® 2500 box
Centrifuge. The instrument must be installed correctly to Power Cord 1 □
ensure proper operation and service. Read this operating
Paper Filter, 1mL 12 □
manual carefully before attempting to operate the
instrument. Follow all instructions carefully. Chamber Holder, 1mL 12 □
The Cyto-Tek 2500 Centrifuge is a precision instrument Specimen Chamber, 1mL 12 □
and must be handled accordingly. Rough handling or 12 □
Chamber Cap, 1mL
dropping of the instrument will disturb or damage internal
components. Always handle the instrument with care. Operating Manual 1 □
NOTE: An English explanatory material on the Japanese

2.2 Installation Requirements warranty card and medical package insert is also
included for overseas customers.
The Cyto-Tek 2500 Centrifuge will deliver optimum
performance if the following requirements are observed: If any of these items are missing contact Sakura Technical
1. Place the centrifuge on a strong, level surface away Support (see Section 5.4).
from any induced mechanical vibration. If there is any visible shipping damage to any item,
2. Do not subject the centrifuge to direct sunlight or any immediately file a complaint with the carrier. Then notify
other form of heat source. your Sakura instrument distributor.
3. The instrument must be in an area of minimal contact
with volatile combustibles and corrosive gases.
4. The instrument should not be on the same electrical 2.4 Unpacking the Instrument
circuit servicing equipment with motors, cooling units,
heating units, or light sources. CAUTION: Use proper lifting techniques when
5. Mark a 30 cm (one foot) boundary around the centrifuge removing the instrument from packaging.
and permit no person or hazardous material within
this zone while the centrifuge is operating. 1. Cut the two plastic straps on the sides of the carton.
2. Remove both cardboard spacers (Figure 2A) and
instrument lid gasket (Figure 2B).
2.3 Unpacking
Open the carton. The carton will contain the following
accessories:
Check
Description Quantity
box
Guard Bowl Unit 1 set □
Medical Package Insert
1 □
(Japan Customers Only)
Warranty Card 1 □
Figure 2A
Unpacking Instructions 1 □

6. Unwrap the proactive vinyl sheet from the instrument
and completely open the lid (Figure 2E).
Figure 2B
3. Remove box tape. Figure 2E

4. Slightly hold up the instrument lid and remove the
foam support inserts (Figure 2C). 7. Remove adhesive tape that holds the guard bowl unit
in place and close the lid (Figure 2F).
Guard bowl unit
Figure 2C
5. Remove the top foam block insert (Figure 2D). Adhesive tape
Figure 2F
8. Grasp the bottom of the instrument and carefully

remove the instrument from the carton (Figure 2G).
Figure 2D
Figure 2G

9. Open the lid by pressing the Lid Open button (Figure 10. Remove a foam cushion (Figure 2J).
2H). Remove the guard bowl unit from the instrument
(Figure 2I).
Lid open push button Foam cushion
Figure 2J
Figure 2H
11. Install the lid gasket, see Section 4.1.3. (Figure 2K).
12. Replace the guard bowl into the instrument
(Figure 2K).
Guard bowl unit
Lid gasket
Guard bowl unit
Figure 2I
Figure 2K

2.5 Standard Accessories and 2.5.2 Options/Consumables

Options ③ ④ ⑥
⑤
2.5.1 Standard Accessories
⑦ ⑨ ⑪
① ②
⑩
⑧ ⑨ ⑩ ⑫
③ ④ ⑤ ⑥
Description/ Product Code

1mL Specimen Chamber Kit, 4301
Includes the following 4 items:
Description/Product Code Quantity  1mL Specimen Chamber
 Power Cord (100/115V), A4010548  1mL Chamber Holder
or 1  1mL Chamber Cap
Power Cord (230V), A4010549
 1mL Paper Filter
 Guard Bowl Unit, F60-755-00
1 set
(Guard Bowl, Rotor, Bowl Lid)  6mL Specimen Chamber, 4331
1mL Specimen Chamber Kit, 4301  12mL Specimen Chamber, 4328
Includes the following items:
 6/12mL Chamber Holder, 4326
 Specimen Chamber, 1mL
12 each  6/12mL Chamber Cap, 4334
 Chamber Holder, 1mL
 6mL Rubber Gasket, 4327
 Chamber Cap, 1mL
 12mL Rubber Gasket, 4337
 Paper Filter, 1mL
Operating Manual, 0007129-01 1
Medical Package Insert (Japan Only) 1
2.5.3 Missing or Damaged Items?

NOTES: Check the contents of all shipping cartons and boxes
carefully for all of the listed items. If any items are visibly
・ The cell deposition area of 1mL paper filter is 6 x 6 damaged, or are missing, call 1-800-725-8723 or 1-310-
mm. 972-7800 (U.S. Customers only). If you are located
・ The cell deposition area of 6mL paper filter and 6mL outside the U.S., contact your nearest Sakura instrument
rubber gasket is 14 x 14 mm. distributor
・ The cell deposition area of 12mL paper filter and 12
mL rubber gasket is 14 x 30 mm.

3.1 Operating Instructions 3.2 Customization of Settings
3.1.1 Workflow (Figure 3A) 3.2.1 Creating a Program
The following is a basic operating flow when using the The instrument starts up in the standby mode after power-
Cyto-Tek® 2500 Centrifuge: on. The control panel displays the program number and
settings of a currently-selected program. The instrument
Assemble the specimen chambers, dispense specimen into each chamber and saves up to 30 programs. Selectable rotation speed is from
install the chamber cap securely.
200 to 2500 rpm, and is displayed in multiples of 10 rpm.
CAUTION: Use microscopic glass slides with ground edges.
CAUTION: Glass slides might break, depending on their size and quality.
Determine if glass slides you want to use can be safely assembled in NOTE: The factory default settings are 1,000 rpm and 10
the chamber holders and processed on the instrument before use.
minutes. Programs can be edited as desired
(Figure 3B).
Load the rotor in the guard bowl with the assembled chambers.
(Balance the rotor while loading).
Put on the bowl lid and lock it.
Open the instrument lid and set the guard bowl unit in place.
Close the instrument lid.

Figure 3B
Select the program number of a program to run, using the UP () and DOWN
() keys. If a desired program does not exist, edit and save a new program.
Setting a Rotation Speed (Figure 3C)
Press the START/STOP key to start the operation.
The lid is locked and the lid lock indicator is lit.
When the operation is ended, the completion buzzer sounds.
After the lid lock indicator has shut off, open the instrument lid.
Remove the guard bowl unit and close the instrument lid. Figure 3C
Remove the bowl lid.

1. Select the program number, then press the SET key.
Remove the specimen chamber holder assemblies from the rotor.
Carefully remove the glass slide from the chamber holder. NOTE: The Speed display starts to flash, indicating that
the speed setting mode is up.
Disinfect or sterilize the guard bowl, bowl lid, and others.
2. Determine the desired rotor speed for the program

Figure 3A
cycle samples. Press the UP () and DOWN ()
keys until the Speed Display indicates the desired
rotor speed. Selectable rotation speed is from 200 to
2500 rpm, and is displayed in multiples of 10 rpm. If
these keys are held down for one second or more, the
value will change by 50.

3. Press the SET key to save the changes and enter the Setting a Process Time (seconds) (Figure 3E)
time (minutes) setting mode.
NOTE: To return to the standby mode without saving the

change, press the START/STOP key.
CAUTION: The rotation speed is selectable from 200
rpm to 2,500 rpm. However, the
morphology of cells may be degraded if a Figure 3E
higher rotation speed is selected.
Consider an appropriate value before 1. Press the SET key.
setting the speed.
2. Press the UP () and DOWN () keys until the
Time Display indicates the desired time in seconds.
Setting a Process Time (minutes) (Figure 3D) 3. Press the SET key to save the changes.
NOTE: To return to the standby mode without saving the

change, press the START/STOP key.
Set and View Mode

When the SET key is held down for 3 seconds or more
Figure 3D and released, the instrument will start the set & view mode
for setting system options and viewing the software
1. Press the SET key. version number and error logs.
2. Press the UP () and DOWN () keys until the This set & view mode provides the following functions:
Time Display indicates the desired time in minutes. • Selecting a completion buzzer
3. Press the SET key to save the changes and enter the • Turning ON or OFF an alert for preventing slides
time (seconds) setting mode. from drying
NOTE: To return to the standby mode without saving the • Selecting the volume
change, press the START/STOP key. • Viewing the software version number
CAUTION: The process time is selectable from 0
• Viewing the error code of an error occurred
minute and 1 second to 99 minutes and
59 seconds. However, a prolonged
process time may dry out specimens.
Consider an appropriate value before
setting the time.

Selecting a Completion Alarm SET-1 (Figure 3F) 60 seconds) to keep the completion buzzer sounding.
The value is displayed in the Time display, like
“On00” or “On60”, and circulated on every key press.
3. Press the SET key to save the change and enter the
sound volume setting mode.
Figure 3F
1. In Standby mode, press the SET key for 3 seconds or Selecting a Sound Volume SET-3 (Figure 3H)
more. The completion buzzer setting mode displays
“SET-1” on the Speed display.
2. Use the UP () and DOWN () keys to change the
value in the Time display from 1 to 3. Each setting is
as follows:
1: (Pi--, Pi--, Pi--)
2: (Pi Pi, Pi Pi, Pi Pi) Figure 3H
3: (PiPiPi--, PiPiPi--, PiPiPi--)
1. Press the SET key in the alert On/Off setting mode.
3. To test the sound in the selected pattern, press the
The sound volume setting mode displays “SET-3” in
START/STOP key. Press the key again to stop the
the Speed display.
buzzer.
4. Press the SET key to save the change and enter the
alert On/Off setting mode. NOTE: Three sound volume levels are available for the
completion and error alarms. Use the UP ()
and DOWN () arrow keys to change the value
Turning an Alert ON or OFF SET-2 (Figure 3G) in the Time display from 1 - 3. Each setting is as
follows:
1: (Low)
2: (Middle)
3: (Loud)
Figure 3G
2. To test the sound volume, press the START/STOP
key. Press the key again to stop the alarm.
1. Press the SET key in the completion alarm setting 3. Press the SET key to save the change and switch to
mode. The alert On/Off setting mode display “SET-2” the software version number display.
in the Speed display.
NOTE: Allows the operator to turn On/Off a End of

Process alarm to prevent slides from drying.
2. Use the UP () and DOWN () arrow keys once at

the end of a run to select “Off” to sound the
completion buzzer. Or select a desired time interval in
one-second increments (selectable from 0 second to

Viewing the Software Version Number (Figure 3I) 3.3 Assembling the Specimen
Chamber and Dispensing the
Specimen
CAUTIONS:
• The 1mL specimen chamber, chamber holder,
Figure 3I chamber cap and paper filter are disposable.
Do not reuse them.
1. Press the SET key in the sound volume setting mode.
The version number of the software currently installed • Confirm that a name and ID on each glass slide
in the instrument is displayed. match those of individual patients or subject.
2. Press the SET key to switch to the error code display. • The limit of rotating imbalance of load is 3
grams. Evenly fill the specimen chambers to be
placed in the rotor.
Viewing Error Codes (Figure 3J)
• Use the 1mL specimen chamber only with the
1mL chamber holder, chamber cap, and paper
filter. Those for the 6mL and 12mL chambers
are not compatible.
• When the paper filter is in use, centrifuge the
chambers within 2 ~ 3 minutes of dispensing
the specimen.
Figure 3J • The 6mL and 12mL specimen chambers, rubber
gaskets, 6/12mL specimen chamber holder and
Error Codes have been programmed into the Cyto-Tek cap are reusable after being disinfected and
2500 Centrifuge. These 2-digit codes alert the operator to washed. However, if they have cracks or are
a possible instrument malfunction or operating error. They deformed, do not use them.
are displayed in the Speed Display window. An alarm
• Do not use glass slides that have flaws. They
buzzer sounds when an error code is displayed.
will be broken during operation.
1. Press the SET key when the software version number
• After the specimen has been dispensed,
is displayed. The error code display appears.
immediately lock the bowl lid and start the
2. The last three errors codes are listed. operation.
• Do not place different types of the chamber
NOTE: The occurrence order of the errors is displayed in assemblies in the rotor at a time. Doing so will
the Program display. The error code of each cause an imbalance error to occur during
error is displayed in the Speed display. If there is operation.
no error stored in memory, the Speed display will
show “E---“. • Glass slides might break, depending on their
size and quality. Determine if glass slides you
want to use can be safely assembled in the
3. Press the SET key to return to the standby mode. chamber holders and processed on the
instrument before use.
• If the specimen chamber using the rubber
gasket is overfilled, aerosoling will occur inside
the guard bowl. If it is under filled, uneven and
inadequate smearing will result.

Procedure for Using 1mL Disposable
Specimen Chambers
1. Write the name of the specimen on a clean glass
slide and place the slide, frosted side up, in the
specimen chamber holder (Figure 3K).
Figure 3N
Figure 3K 5. Prepare other specimen chamber assemblies.

6. Hold the specimen chamber holder vertically, so that
2. Place the new paper filter on the slide (Figure 3L). the graduations of 0.5 mL and 1.0 mL, marked on the
cylindrical portion of the specimen chamber are
clearly visible. Dispense from 0.1 mL to 1.0 mL of
specimen into the specimen chamber. Then tightly
seal the specimen chamber with a specimen chamber
cap (Figure 3O).
Chamber cap
Figure 3L
3. Insert the two tabs at the bottom of a clean specimen

chamber into the corresponding holes of the chamber 1.0mL
holder, as shown below (Figure 3M).
0.5mL
Figure 3O
NOTE 1: Do not fill the 1.0 mL specimen holder with

more than 1.0 mL of specimen, as excess
specimen may aerosol into the rotor bowl.
NOTE 2: A better dispersion of cells on the glass slide
may be obtained by diluting small volume
samples (0.3 mL or less) to a final volume of
0.5 mL with an appropriate diluent.
Figure 3M
4. Align the square hole of the paper filter with the hole
in the specimen chamber holder. Press the top of the
specimen chamber back toward the specimen
chamber holder, flatting it out, until it engages the
restraining latch on the top of the specimen chamber
holder (Figure 3N).

Handling of 6mL and 12mL Specimen

Chambers 6/12mL chamber cap (option)
1. Write the name of the specimen on a clean glass

slide and place the slide, frosted side up, in a
NOTE: An optional rubber
specimen chamber holder. chamber cap is available for
2. Place the 6mL rubber gasket, or 6mL paper filter, on centrifugation of infectious
specimens.
the glass slide to use for the 6mL specimen chamber.
Similarly, to use for the 12mL specimen chamber,
Level line
place the 12mL rubber gasket, or 12mL paper filter,
on the glass slide (Figure 3P). Figure 3R
The illustration is the 6mL chamber.
3.4 How to Load the Rotor

6mL rubber gasket CAUTION: In order to minimize a risk of infection,
place the guard bowl inside a biological
Figure 3P safety hood when installing or removing
the bowl lid.
3. Insert a specimen chamber into the bottom of the CAUTION: Confirm that a name and ID on each glass
chamber holder. Press in firmly to properly seat the slide match those of individual patients
specimen chamber. Press the chamber holder clip or subject.
down to make it engage the hooks at each side of the
chamber holder (Figure 3Q). CAUTION: Load the Cyto-Tek Centrifuge rotor so
that it remains balanced. An improperly
The illustration is the 6mL chamber.
loaded, unbalanced rotor will cause the
6mL specimen chamber centrifuge to vibrate excessively. This
can shorten the life of the instrument.
Clip
Hooks
1. Open the instrument lid to remove the guard bowl
unit.
2. Place the guard bowl inside a safety cabinet. To
Figure 3Q
unlock the lid, pull the lock button up on the top of the
lid (Figure 3S). Remove the lid (Figure 3T).
Align the opening of the specimen chamber with the
opening of the rubber gasket, or the paper filter.
4. Dispense the specimen into the specimen chamber
up to the level line (6mL or 12mL). Fill the specimen
chamber exactly to the level line. (Figure 3R).
Figure 3S

Figure 3T Figure 3W
3. Load the rotor with an even number of assembled 5. Place the lid back on the rotor and press down on the
chambers. Arrange them in the rotor so that pairs of button with your fingers to lock the lid (Figure 3X).
specimen chamber holder assemblies are opposite
one another (Figure 3U).
Figure 3X
Figure 3U
CAUTION: Make sure that the bowl lid is firmly
4. If there are an odd number of specimen samples to locked.
process, maintain the rotor balance by adding one
CAUTION: If the guard bowl unit needs to be
specimen chamber filled with water to pair with the
transferred with the specimen-loaded
last unpaired specimen.
chambers set in the rotor, be sure to lock
the bowl lid and carefully carry the guard
CAUTION: Be sure to orient the tab of the chamber bowl with both hands.
cap so that it will not make contact with
the edge of the rotor compartment, as
shown by arrows (Figure 3V and 3W).
Figure 3V

process time may make specimens dry.
3.5 Starting the Operation Consider an appropriate process time
before setting the time.
1. Set the power switch to the ON position.
CAUTION: After the start of operation, make sure
2. Place the guard bowl into the instrument and close
that the rotation speed reaches the set
the instrument lid (Figure 3Y).
value and is stable.
3.6 Discontinuing the Operation

To discontinue the operation, press the START/STOP
key.
The instrument will start decelerating the motor and unlock
the instrument lid when the rotation speed reaches 50rpm
or less. An alarm will sound to the operator that the
instrument has stopped.
NOTE: While the lid is unlocked, the time left is still

Figure 3Y
displayed in the window. The operation, however,
cannot be restarted with the time left. When the lid
is opened, the instrument returns to the standby
mode and resets the displayed time to zero.
3.7 Emergency Stop

To stop the operation immediately, switch off the
instrument (Figure 3AA).
① ②
Figure 3Z
3. Press the UP () and DOWN () arrow keys () until
the desired program number is obtained (Figure 3Z).
Power switch
NOTE: If a desired program does not exist, make
changes to create a new program.
4. Press the START/STOP key () to start the Figure 3AA

operation (Figure 3Z).
NOTE: The instrument lid is locked during operation.

CAUTION: The process time is selectable from 0
minute and 1 second to 99 minutes and
59 seconds. However, a prolonged

Dispose of the used 1mL specimen chambers,
3.8 Removing the Guard Bowl chamber holders, chamber caps and filter paper, as
appropriate according to the specimen processed or
CAUTION: In order to minimize a risk of infection, reagents used, as they are disposable.
place the guard bowl inside a biological
safety hood when installing or removing
the bowl lid. CAUTION: Drain the fluid waste accumulated in the
guard bowl after every run.
CAUTION: Do not leave the bowl lid locked after use.
1. When the operation has ended, a buzzer sounds to
inform the operator the run has completed. Open the If the pressure inside the guard bowl
instrument lid and remove the guard bowl. becomes negative, the lid will become
difficult to remove.
2. Place the guard bowl inside a safety cabinet. Pull up
the lock button to unlock the bowl lid and remove
(Figures 3BB and 3CC).
Figure 3BB
Figure 3CC
3. Remove the specimen chamber holders from the

rotor.
4. Remove the glass slide from the chamber holder. Do
not to let the smeared area go out of alignment.
5. Drain the guard bowl of fluid waste. Disinfect or
sterilize the guard bowl and bowl lid.
Disinfect the 6mL or 12mL specimen chambers,
chamber caps, rubber gaskets, and 6/12mL chamber
holders after use. See Section 4.1.4 for cleaning
instructions.


4.1 General Information 4.1.3 Disassembling and Cleaning
the Guard Bowl Unit
®
The Cyto-Tek 2500 Centrifuge is a precision instrument
designed for trouble-free operation and requires minimal
routine maintenance. Handle the instrument with care to CAUTION: To lessen the infection risk, place the
avoid damage to the sensitive electronics. The centrifuge guard bowl unit inside a safety cabinet
may be kept in good operating condition by carefully before removing and the lid.
following these instructions. Before cleaning or performing
maintenance on the instrument, place the ON-OFF Switch To clean, disinfect, or sterilize the guard bowl unit, it is
in the OFF position and unplug the power cord from its necessary to place and disassemble it inside a safety
electrical outlet. cabinet. Remove the lid gasket from the lid and the rotor
unit and the V-shape gasket from the guard bowl before
WARNING: WHEN PERFORMING MAINTENANCE OR cleaning.
CLEANING THE INSTRUMENT, STANDARD
LABORATORY SAFETY PROCEDURES Disassembly of the bowl lid
AND PERSONAL PROTECTIVE EQUIPMENT 1. Remove the bowl lid (Figure 4A).
SHOULD BE USED.
4.1.1 Cleaning the Control Panel

1. Wipe the internal surfaces (including the inside lid
area and the well) with a clean cloth moistened with
neutral detergent. Pat dry any excessively moist
areas.
CAUTION: Do not use volatile liquids such as Figure 4A

solvents.
2. Remove the lid gasket (Figure 4B).
4.1.2 Cleaning the Instrument

1. Wipe the external surfaces (Lid Gasket area and
Guard Bowl Storage area) with a clean cloth
moistened with 70-80% ethyl alcohol or isopropyl
alcohol for disinfection. Pat dry any excessively moist
areas.
CAUTION: Do not spray a disinfectant directly on or

over the instrument.
Figure 4B

Disassembly of the Guard Bowl The guard bowl unit has been disassembled as shown
below (Figure 4F).
1. Loosen two screws that hold the rotor unit in place. Guard Bowl
Remove the rotor unit (Figure 4C and 4D). Bowl Lid
V-shape
Figure 4C
Rotor Bowl Lid Gasket
Figure 4F
Assembly of the Guard Bowl Unit

1. Install the V-shape gasket (Figure 4G and 4H).
Figure 4D
2. Remove the V-shape gasket (Figure 4E).

Figure 4G
V-shape gasket
Bowl shaft
housing
Figure 4H
CAUTION: Install the V-shape gasket on the bowl

shaft housing with the projecting part of
the gasket facing upward. Failure to do
Figure 4E
so may weaken the seal capacity.

2. Install the bowl lid gasket to the bowl lid (Figure 4I). Installation (replacement) of the
Instrument Lid Gasket (Figure 4K)
Lid
Gasket
Instrument
Lid
<Section View>
“UPSIDE”
on this surface
Bowl Lid Gasket Figure 4K
1. Open the instrument lid and pull out the old lid gasket
Bowl Lid
from the groove.
2. Wipe clean the gasket groove with a cloth or paper
towel.
Figure 4I 3. Confirm which side of the gasket faces inward. Direct
the projecting part of the gasket to the back side of
CAUTION: Install the bowl lid gasket to the bowl lid the groove as illustrated in the above section view.
with the “UPSIDE” facing upward. 4. Determine two points a and b so that the gasket is in
CAUTION: Install the bowl lid gasket so that it is exact halves. Place the point a at the top of the
evenly applied around the bowl lid gasket groove and the point b at the bottom and fit
overall. Failure to do so may weaken the two points of the gasket into the groove. Next,
seal capacity. determine two points c and d so that the upper and
lower lengths of the gasket become the same. Fit
3. Place the rotor unit into the guard bowl and tighten these points into each side of the groove in an
the two screws (Figure 4J). appropriate place. Insert other parts of the gasket into
the groove evenly (Figure 4L).
Point a
Gasket Point
Point c Point d
Lid gasket
Point b Point b
Point a
Point c Point d
Figure 4J
Point b Figure 4L

5. Run your fingertip along the surface of the gasket. 4.1.4 Cleaning 6mL/12mL specimen
Push the gasket into the groove where necessary.
chambers, 6mL/12mL rubber
List of Disinfection/Sterilization Methods gaskets, 6mL/12mL chamber cap and
chamber holder (Figure 4M)
CAUTION: When sterilizing the guard bowl unit by
high pressure steam, wear protective These assemblies consist of the following four parts:
clothing like gloves and take care not to  specimen chamber holder
get burned.  rubber gasket
CAUTION: All of the components of the guard bowl  specimen chamber
are autoclaveable. Each component must  specimen chamber cap
be sterilized separately.
CAUTION: If the bowl lid, rotor and guard bowl are
placed in the same container for
disinfection, they may be deteriorated
faster (dissimilar metal corrosion). It is
recommended to disinfect them
separately.
Component Disinfection/Sterilization
Bowl Lid Can be sterilized in an autoclave (121°C, 20
minutes).
Can be disinfected by soaking in ethyl alcohol
(80%), isopropyl alcohol (70%) or sodium
hypochlorite solution (0.5% or less in
concentration, for less than 20 minutes).
Rotor Can be sterilized in an autoclave (121°C, 20

minutes).
hypochlorite solution (0.5% or less in
Guard Bowl Can be sterilized in an autoclave (121°C, 20

minutes).
hypochlorite solution (0.5% or less in Figure 4M
CAUTION: All these components should be carefully decontaminated
If the guard bowl is autoclaved as it is and cleaned prior to use.
moistened with sodium hypochlorite 1. All components should be soaked in an approved
solution, it will be wear down faster.
disinfectant. A 0.5% chlorhexidine hydrochloride
Bowl Lid Can be sterilized in an autoclave (121°C, 20 solution or a 0.5% or less sodium hypochlorite
Gasket minutes). solution (common bleach) are recommended
Can be disinfected by soaking in ethyl alcohol disinfectants.
V-shape
Gasket (80%), isopropyl alcohol (70%) or sodium 2. After soaking the components in an approved
hypochlorite solution (0.5% or less in disinfectant, they should be cleaned in hot water and

detergent. The water temperature should not exceed 4.1.5 Gasket Replacement
40° C (104° F). Rinse the cleaned items thoroughly in
clear water and drain to dry. Replace the bowl lid gasket and V-shape gasket when
they get deteriorated or at least once a year.
3. The stainless steel specimen chamber holders 
may be soaked in an approved disinfectant for a When installing replacement gasket, note the correct
maximum of 10 minutes, followed by rinsing orientation of the gasket.
thoroughly in hot tap water. These specimen chamber
holders  may be autoclaved as an alternative to the 4.1.6 Preventive Maintenance by a
cleaning procedure. Service Contractor
Do not autoclave the plastic and rubber components The following components can be inspected and replaced
[rubber gasket , specimen chamber , and specimen as necessary. For more details, contact our Technical
chamber cap ] and do not expose these items to Support Department or your nearest instrument distributor.
xylene, thinner, or any solvent. Do not store these items in
direct sunlight or near any source of heat. The chamber ・Guard bowl unit
used to hold the disinfectant for soaking the components ・Imbalance detection mechanism
may be glass or metal, but not plastic. Be careful not to ・Lid lock mechanism
cause any abrasion to the inner portion of the chamber or
the raised portion around the exit port hole of the ・Lid opening/closing mechanism
chamber. Abrasions could have a negative impact on cell ・Controller board
harvest by providing a niche (capture space) for the cells ・Gaskets and consumables
on their way to being deposited on the slide.
CAUTION: The 6mL and 12mL specimen chambers,

rubber gaskets, 6/12mL specimen
chamber holder and cap are reusable
after disinfected and rinsed. However, if
they have cracked or warped, do not use
them.
Decontamination
If any hazardous material is spilled on or inside the
instrument, decontaminate the instrument by following the
cleaning procedures contained this Section, Care of the
Instrument.
Transportation
Disinfect the instrument before transporting. See above,
Decontamination.
Please call the Sakura Technical Support Department at
1-800-725-8723, option 2.
If located outside the United States, contact the nearest
Sakura instrument distributor or representative for
information and assistance


WARNING: BEFORE OPENING THE INSTRUMENT

5.1 Power Outage LID AFTER IT IS UNLOCKED, TAKE
INFECTION CONTROL MEASURES SUCH
Unlock the Lid Mechanically During Power AS WEARING PROTECTIVE CLOTHING
Outage AND PLACING THE INSTRUMENT IN A
SAFETY CABINET.
WARNING: MAKE SURE THAT THE ROTATION HAS
STOPPED BEFORE PERFORMING THE
WORK.
5.2 Actions to be Taken When an
In the event of a power outage, the instrument lid will Error Occurs
remain locked. The lid can be manually opened, if needed.
1. Set the power switch to the OFF position. If an error occurs during operation, the instrument
2. Remove a small cap located on the left side of the immediately decelerates the motor and sounds the alarm.
instrument (Figure 5A) After the motor has stopped, the lid lock indicator (LID
LOCK) is turned off. Open the instrument lid and take
corrective actions for the error occurred.
The alarm buzzer can be silenced by pressing any key on
the control panel (START/STOP, SET, UP and DOWN
keys).
To exit from the error display, turn the instrument power
off and on again.
Cap
Description of the Error Display
Figure 5A
(Figure 5C)
3. Insert a bar for unlocking the lid into the hole
(Figure 5B).
Display of the error code of an error
Figure 5B
4. Push in the bar to release the lock mechanism. The

lid can be opened.
Figure 5C Display of balance error
CAUTION: Use a rigid bar that is 4 to 6 mm across
and more than 5cm long.

1. The error code of an error is displayed in the Speed
display window.
2. Up to three errors can be viewed with the latest one
first. The occurrence order is displayed in the
Program display window like “1”, “2” and “3”. If the
same error occurs successively, it is only registered
once. If multiple errors occur during one run, only
one error that has the highest priority is registered.
3. The error code is displayed as “E-xx“, except for the
balance error which is displayed as “bAL”. For
details, see the following tables.
5.3 Troubleshooting Charts

The following Troubleshooting Charts list caution
messages and error codes that could occur during
operation of the Cyto-Tek® 2500 Centrifuge. Possible
problems could be electrical, operational, or mechanical.
Probable causes and recommended remedies are also
included so that isolated problems can be quickly
corrected.
If additional assistance is required concerning an
instrument problem, an error code displays that is not
listed here, or if the problem cannot be isolated or is
beyond the scope of this manual, complete the
“PreService Checklist” at the end of this section. Then
contact the Technical Support Department of Sakura
Finetek USA., Inc. by calling toll free 1-800-725-8723,
option 2 (U.S. only). If located outside the United States,
contact the nearest Sakura instrument distributor or
representative for information and assistance.

5.3.1 Error Code of Monitored Errors
Error Description/Symptom Major Causes Action(s)

Code
Faulty solenoid or wiring
Contact Sakura Technical Support or distributor.
Poor connector contact To remove specimen chambers, see “How to
85 The instrument lid remains locked
Controller board failure unlock the lid mechanically during power outage”,
P2-15.
Lid lock detection sensor failure
Poor connector contact

86 The instrument lid cannot be locked Contact Sakura Technical Support or distributor.
Controller board failure
Faulty lid sensor or improper wiring
The instrument lid is opened during Poor connector contact

87 Contact Sakura Technical Support or distributor.
operation. Controller board failure
The lock was released during Poor connector contact

operation Controller board failure
The instrument lid is open but the Poor connector contact

solenoid is in the locked condition. Controller board failure

Error Description/Symptom Major Causes Action(s)
Code
Improper guard bowl placement Place the guard bowl properly.
Put the bowl lid on the guard bowl and lock
A run is started without the bowl lid put on
Rotation detection error occurred properly.
94 during acceleration Poor connector contact
(the rotation speed does not increase)
Controller board failure Contact Sakura Technical Support or distributor.
Faulty motor, motor driver or wiring
Place the guard bowl properly Place the guard bowl properly.
Rotation detection error occurred
during constant rotation Poor connector contact
95
(the motor speed deviated ±100rpm Controller board failure Technical Support or distributor.
from the set value)
Improper guard bowl placement Place the guard bowl properly.

Rotation detection error occurred
during deceleration Poor connector contact
96 Place the guard bowl properly. Contact Sakura
(the rotation speed does not decrease Controller board failure
during deceleration) Technical Support or distributor.
Temperature error Turn the instrument power off. Operate the

instrument again in 30 minutes. If the same error
Locked motor
occurs, contact Sakura Technical Support or
Motor error Overload distributor.
97 (the motor’s alarm function was
triggered) Faulty motor, motor driver or wiring
Controller board failure Contact Sakura Technical Support or distributor.
Specimen chambers are not properly arranged in Always arrange the specimen chambers
the rotor. symmetrically.
Specimen volume in each specimen chamber is not
Fill the specimen chambers evenly.
even.
Eccentricity of motor shaft and/or spindle
bAL Balance error
Distortion of guard bowl and/or rotor
Improperly adjusted balance sensor
Rotation mechanism failure

Fluid is accumulated in the guard bowl (when paper
Drain the guard bowl of accumulated fluid.
filters are used).

5.3.2 Other Errors
Problem Major Causes Remedy
Inspect power source, power plug and power switch.
Power was lost/interrupted during operation (due to
The lid lock is released when the rotation speed goes
power failure, etc.).
below ±50rpm after power restoration.
The instrument lid cannot be
Instrument lid push button failure
opened. Contact Sakura Technical Support or distributor.
Faulty spring(s) To remove specimen chambers, see “How to unlock the
lid mechanically during power outage”.
Worn bushing
The bowl lid is locked. Unlock the bowl lid.

Warm the guard bowl and open the lid.
The pressure inside the guard bowl is negative. If the problem persists, contact Sakura Technical
The bowl lid cannot be opened. Support or distributor.
Bowl lid lock/unlock mechanism failure Warm the guard bowl and open the lid.
If the problem persists, contact Sakura Technical
The guard bowl is distorted. Support or distributor. Contact Sakura Technical Support
or distributor.
Keys on the control panel do not Faulty membrane switch
react. Control panel board failure
Power supply facility failure Check if power supply on the facility side is normal.
Faulty power cord
The power is not supplied. Switching power supply failure Confirm that the power cord is firmly plugged.
Leakage in switching power supply Contact Sakura Technical Support or distributor.
Blown fuse(s)
The grounding wire to the power input is disconnected. Make sure that grounding wire is firmly connected.
An electric shock occurred.
Leakage in the power input area Contact Sakura Technical Support or distributor.
Poor cable contact
Control panel board failure

The 7-segment display is broken
or flickering. 7-segment display malfunction

LED indicator is not illuminated. Faulty membrane switch Contact Sakura Technical Support or distributor.
Error/warning/click sounds are
Control panel board failure Contact Sakura Technical Support or distributor.
not generated.
Specimen chambers are not properly arranged in the
Always arrange the specimen chambers symmetrically.
rotor.
Specimen volume in each specimen chamber is not
Fill the specimen chambers evenly.
even.
Abnormal noise is heard. Eccentricity of motor shaft and/or spindle

The outer casing surface Distortion of guard bowl and/or rotor
vibrates abnormally. Contact Sakura Technical Support or distributor.
Improperly adjusted balance sensor
Loose mounting screws in each part

Place the instrument on a sturdy laboratory table or
Improper instrument installation
bench.

®
5.4 Service Information 5.4.2 Cyto-Tek Centrifuge
PreService Checklist
5.4.1 When there is a Problem with Serial Number: ________________________________
the Instrument Date of Installation: _____________________________
Model Number: ________________________________
Avoid problems by carefully following proper operating Warranty Period Date:___________________________
and cleaning procedures for the Cyto-Tek® Centrifuge and
the specimen chamber holders. If problems arise during
the operation of the instrument, first refer to 1. Is the power cord plugged into an appropriate power
TROUBLESHOOTING Section. source?
If the problem cannot be solved and an instrument failure 2. When the power switch is turned on, is the display
is apparent, our Technical Support Department is panel illuminated and are the Mode lights lit?
available to assist you. Before calling for instrument 3. Is there electricity with the correct voltage in the outlet
service, collect the information requested in the the instrument is plugged into? If not, check the
“PreService Checklist” of this section. This information will corresponding fuse or circuit breaker.
help the Technical Support Representative identify the 4. What Error Codes(s), if any, are indicated on the
probable cause of the instrument malfunction. The control panel?
instrument should be disinfected prior to being serviced 5. Was the centrifugation interrupted for any reason
(See Section 4, CARE OF THE INSTRUMENT, for after START had been pressed?
disinfection instructions).
6. Was there a power failure during operation?
Where to Call for Service
If located within the United States, contact the
Technical Support Department of Sakura Finetek USA,
Inc. by calling toll free:
1-800-725-8723, option 2.
In countries other than the United States, contact the

nearest authorized Sakura instrument distributor or
representative for service information and assistance.

5.4 Accessory Items
Product Number/Description
4301 Specimen Chambers Kits (1 mL)-Disposable
4331 Specimen Chambers (6 mL)
4328 Specimen Chambers (12 mL)
4334 Specimen Chamber Caps (6 mL or 12 mL)
4326 Specimen Chamber Holder with Clip Stainless
Steel (6 mL or 12 mL)
4327 Silicon Rubber Gasket (6 mL)
4337 Silicon Rubber Gasket (12 mL)
5.5 Replacement Parts

Part Number/Description
F60-755-00 Guard Bowl Unit
F60-895-00 Bowl Lid
F60-896-00 Bowl Unit
F60-97-00 Rotor Unit
A3-400-048 Time Delay Fuse, HT 2AN5
B3-01-0000 V-Ring
0007129-01 Operating Manual
5.6 Where to Order

If located within the United States Accessory Items and
Replacement Parts are available directly from:
Order Management
Sakura Finetek USA, Inc.
1750 West 214th Street
Torrance, CA 90501 U.S.A.
1-800-725-8723, option 1
Or contact the nearest authorized Sakura distributor or
representative. In countries other than the United States,
contact the nearest authorized Sakura distributor or
representative for service information and assistance.


6.1 Safety Precautions A label bearing this symbol specifies a
cautionary item. Improper handling against
the instruction may endanger the operator
Operation of this instrument requires expert knowledge of or damage the instrument. Always follow
the intended application, instructions for use, etc. the instruction.
Therefore, appoint a person responsible for operation of
the instrument in order to use it properly and safely.
When the instrument is delivered, the responsible person
should receive full explanation on the handling of the
instrument from our sales representative.
・ Read this “Safety Precautions” beforehand to operate
the instrument properly.
・ The cautionary instructions provided herein are
intended to secure safety operation of the instrument
and prevent physical damage and injury to the
operator. Follow these important safety instructions
fully.
A label bearing this symbol requires the
・ In this manual, potential hazards at different levels are operator or other personnel to wear
classified as “Danger”, “Warning”, “Caution” and “Note” protective clothing for infection control
indicated respectively where applicable. They are when using, inspecting, or disassembling
defined as follows. any part of the instrument where this label
<Danger> identifies a potential hazard in which failure to follow is attached. Also, wash hands thoroughly
instructions may result in death or serious injury to after use of the instrument.
the operator and/or other personnel.
<Warning> indicates a potential hazard in which failure to follow
instructions may result in serious injury to the
operator and/or other personnel.
<Caution> indicates a potential hazard in which failure to follow
instructions may result in injury to the operator
and/or other personnel or damage to the instrument
and/or other property.
<Note> indicates a reminder or other helpful information.
・ Images shown in this manual may be partially different

from those of the actual instrument.
・ Please note that we are not responsible for any effect of
the slides prepared by the instrument on diagnosis or
any effect derived from the instrument’s specification
on specimens and slides.

6.1.1 Dangers Do not connect to a piggybacked electric outlet.

Do not use an extension cord.
Do not wet the instrument. Doing so may cause breakdown by low voltage or fire due
Wetting any part of the instrument other than those specified in to heat generation.
“Daily Care and Maintenance” or other applicable sections may
cause fire or electric shock due to leak current. Do not use with incorrect power voltage.
Do not power the instrument if it is not free from Do not alter power supply components and
dew condensation. cables.
Powering the instrument with condensation formed may cause Do not use improper fuses.
fire or electric shock due to leak current.
Fuses must be replaced by authorized service
Do not bring fire sources close to the instrument.
personnel.
Do not disassemble or modify the instrument.
The instrument may malfunction or cause an accident.
Contact Sakura Technical Support when needed.
Do not pinch your hand between gaps in movable Unplug the instrument before replacing fuses.
Failure to do so may cause the instrument to overheat or may
components. lead to electric shock, short circuit, or accidental fire. Check for
Your hand may be injured when pinched. working voltage prior to operation of the instrument.
Do not operate the switches with wet hands or Do not damage the power cord.
connect/disconnect the power plug with wet Do not cut, break, modify, forcibly bend, twist, or bundle the
hands. Connect/disconnect the power plug by power cord. Do not put heavy things on the power cord or store
holding the plug. in a small place. Doing so may damage the power cord, leading
Doing so may result in electric shock. to fire or electric shock.
Use only the supplied power cord. Do not locate the instrument in a place exposed
Use of a power cord with inadequate current capacity may to direct sunlight or possibly getting wet with
cause electric shock or fire by short circuit. Use the power cord splashes from rain or snow.
appropriate to your local current capacity. Ultraviolet rays or extreme temperature may cause breakdown.
Regional safety regulations may require using a Operate the instrument in a clean, less humid
standard power cord designated by statute. If any place.
other power cord than a PSE-certified or UL/CSA- The instrument is intended to be operated indoors. Do not use it
approved or European-made power cord is outdoors.
needed, contact the nearest instrument Do not use the instrument in a place under
distributor. following conditions:
・ A place where it will be subjected to low or high temperatures
Connect the power plug to an outlet with a (allowable operating range is from 10°C to 40°C).
grounding terminal.
・ A humid place where condensation can build.
Connect the power plug to a dedicated, D-class grounded power
receptacle. Failure to do so may cause fire or electric shock ・ A place exposed to a lot of dust, oily smoke or steam.
due to leak current. ・ Outdoors.
In the event of system malfunction, turn off the ・ If the instrument needs to be installed in a special place,
power and then remove the power plug from the contact a Sakura sales representative.
power outlet. Be sure to open and close the guard bowl or
In the event of instrument malfunction, turn off the instrument handle specimens in a safety cabinet.
power and call Sakura Technical Support Center. Unplugging Specimens should be regarded as biologically hazardous. Use
the instrument can also turn off the main power supply. If the of the components like the guard bowl for biological safety may
instrument malfunctions, remove the power plug from the power not be enough to prevent contamination with pathogenic
receptacle. At instrument installation, choose such a place as a organisms.
wall outlet is not hidden behind the instrument so that you can The instrument and guard bowl, however, are not resistant to an
quickly unplug the instrument in the event of emergency. UV germicidal lamp or ozone.

6.1.2 Warnings Without these labels, the operator cannot be reminded of
necessary warnings and cautions for daily handling of the
Avoid using deteriorated or broken or deformed instrument, which may result in an accident or injury.
components, for instance, a specimen chamber, Do not place obstruction on movable
chamber holder, guard bowl and gaskets. components.
Do not open the instrument lid when the rotor Do not spill any organic solvent on the
(guard bowl) is rotating. instrument.
Doing so may result in an accident or injury.
Open and close the guard bowl lid and
Do not move the instrument when the rotor instrument lid slowly.
(guard bowl) is rotating.
Doing so may result in accident or injury. After the rotor has
stopped, unplug the power cord before moving the instrument.
6.1.3 Cautions
Arrange specimen chambers diagonal to each
other when placing them into the rotor. Be careful not to pinch your hand when opening
Loading only one or an odd number of specimen chambers will or closing the instrument lid.
cause the rotor to become unbalanced during rotation, resulting Do not make a strong impact on the transparent
in abnormal, dangerous vibration. In the event of abnormal
vibration, immediately switch off the instrument. window.
Doing so may break the transparent window and cause injury.
Avoid selecting a longer process time.
The process time is selectable from 0 minute and 1 second to Do not remove warning/caution labels from the
99 minutes and 59 seconds. However, a prolonged process time instrument.
may make specimens dry. Consider an appropriate process Without these labels, operator cannot be reminded of necessary
time before setting the time. warnings and cautions for daily handling of the instrument,
which may result in an accident or injury.
Connect the power cord firmly
Loose connections may cause the connected part to generate When sterilizing the guard bowl unit by high
heat or the voltage to decline, leading to not only improper pressure steam, wear protective clothing like
operation of the instrument but also unexpected accident or gloves and avoid getting burned.
injury.
When disinfecting the exterior and interior of the
Turn off the power before performing cleaning.
instrument, wear protective clothing and place
Turn off the power before performing cleaning unless
otherwise instructed in this manual. the instrument inside a safety chamber.
After sterilized by an autoclave, the guard bowl Do not use the disinfectants other than listed
and other components will be very hot. Be below. Doing so may result in an accident.
・ Cleaning ethyl alcohol (80% or less)
careful not to get burned.
・ Cleaning isopropyl alcohol (70% or less)
Use specified fuses.
・ Sodium hypochlorite solution (0.5% or less, within 20
Place the instrument on a sturdy laboratory table minutes)
or bench. Do not block the ventilation ports of the
Failure to do so may result injury or accident.
instrument.
Install the instrument in a horizontal and vertical Blocking the ventilation ports may cause improper ventilation
posture. and instrument failure.
The instrument in a slanted posture may lead to an accident. Do not put an object and reagent on top of the
Leave 30cm or more of clearance around the instrument.
instrument. Doing so may cause an accident. Spillage of a chemical
solution may damage or break down the instrument.
Do not remove caution labels from the
instrument.

Open and close the instrument lid and guard If the instrument is not used for a long period, clean the
instrument lightly, put the dust cover on to avoid dust and
bowl lid slowly.
foreign matter, and store in a place with low humidity.
A strong impact may break the lid.
Do not open the instrument lid unless needed. Perform a periodic inspection once a year.
In order to operate the instrument safely and maintain its
Open the instrument lid only when needed to operate the
capabilities, conduct a periodic inspection once a year. Contact
instrument as instructed in this manual. The time the lid is
a Sakura sales representative for details
opened should be as short as possible.
Perform a test run before determining operating Do not clean the instrument with benzene or
parameters. thinner.
Do not wipe the instrument surface with benzene or thinner.
Operating parameters vary depending on specimen type. Test
Doing so may cause the surface to dissolve or crack or its
operating parameters on the instrument actually before
rigidity to decrease. Wipe with a paper towel moistened with
determining them.
alcohol. Do not spray with alcohol. Fluids running into electric
Use designated instrument accessories and components may cause insulation failure or deterioration like
consumables. corrosion.
Use consumables specified in this manual for replacement. Use Use specified cleaning agents.
of wrong components will cause malfunction or failure. Use a mild detergent. If an unspecified agent is used, the
Do not spill fluids like specimens or water on the instrument may corrode or malfunction.
lid latch hole and the control panel. Exercise due caution when handling reagents.
Wipe spillage off immediately. Wear gloves, mask and/or safety goggles, or take
Do not put foreign matter into the lid latch hole other proper measures designed to protect the
and the louver on the back and sides of the operator, by following the applicable regulation
instrument. or guideline in your country or region.
Doing so may cause instrument malfunction.
Some reagents are a hazard to human health.
Always keep the spindle and bowl shaft housing If any biologically hazardous substance is
clean and dust-free. handled, follow applicable regulations or
guidelines in your country or region to ensure
Keep the guard bowl unit removed from the safety. Wear protective clothing and work in a safety
instrument when it is not in use. cabinet as appropriate to protect the operator. Sterilize
Failure to do so may cause the guard bowl to seize and make it possible infectious waste and infectious medical waste and
unable to remove. dispose of them properly as per regulations and guidelines
Use microscopic glass slides (edge-ground). in your country or region
Glass slides might break, depending on their size and quality.
Determine if glass slides can be safely assembled in the
chamber holders and processed on the instrument before use.
Confirm that a name and ID on each glass slide
match those of individual patients or subjects.
Do not leave the guard bowl lid locked after use.
The bowl lid left locked after use may become difficult to remove
if pressure inside the guard bowl becomes negative.
Do not put an obstruction on movable
components.
Doing so may cause instrument failure.
Store the instrument with the power cord
unplugged for extended non-use.

Operating Manual Cyto-Tek 2500 Cytocentrifuge (GB)

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Cyto-Tek 2500

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2. INSTALLATION AND SET UP

4. CARE OF THE INSTRUMENT

Page - 1 -, Revised 31 March 2014

5. TROUBLESHOOTING AND SERVICE

Page - 2 -, Revised 31 March 2014

Page 3, Revised 31 March 2014

The guard bowl unit is comprised of the guard bowl, rotor,

Page 4, Revised 31 March 2014

⑤ Program Number display (7-segment display)

⑥ Speed display (7-segment display)

⑦ Time display (7-segment display)

⑧ Lid Lock indicator

Page 5, Revised 31 March 2014

1.4 Specifications Operating Conditions:

Programmable Time Settings:

Page 6, Revised 31 March 2014

COMPARISION OF ROTATION SPEED RELATIVE TO

Page 7, Revised 31 March 2014

Page 8, Revised 31 March 2014

NOTE: An English explanatory material on the Japanese

Page 9, Revised 31 March 2014

3. Remove box tape. Figure 2E

8. Grasp the bottom of the instrument and carefully

Page 10, Revised 31 March 2014

Lid open push button Foam cushion

Guard bowl unit

Page 11, Revised 31 March 2014

2.5 Standard Accessories and 2.5.2 Options/Consumables

Description/ Product Code

2.5.3 Missing or Damaged Items?

Page 12, Revised 31 March 2014

Put on the bowl lid and lock it.

Close the instrument lid.

When the operation is ended, the completion buzzer sounds.

Remove the bowl lid.

2. Determine the desired rotor speed for the program

Page 13, Revised 31 March 2014

NOTE: To return to the standby mode without saving the

NOTE: To return to the standby mode without saving the

Set and View Mode

Page 14, Revised 31 March 2014

NOTE: Allows the operator to turn On/Off a End of

2. Use the UP () and DOWN () arrow keys once at

Page 15, Revised 31 March 2014

Page 16, Revised 31 March 2014

Figure 3K 5. Prepare other specimen chamber assemblies.

3. Insert the two tabs at the bottom of a clean specimen

NOTE 1: Do not fill the 1.0 mL specimen holder with

Page 17, Revised 31 March 2014

Handling of 6mL and 12mL Specimen

1. Write the name of the specimen on a clean glass

3.4 How to Load the Rotor

Page 18, Revised 31 March 2014

Page 19, Revised 31 March 2014

3.6 Discontinuing the Operation

NOTE: While the lid is unlocked, the time left is still

3.7 Emergency Stop

4. Press the START/STOP key () to start the Figure 3AA