Experiment No. 1 The Compound Microscope Objectives: Materials: Observation
Experiment No. 1 The Compound Microscope Objectives: Materials: Observation
Experiment No. 1 The Compound Microscope Objectives: Materials: Observation
College of Criminology
Experiment No. 1
The Compound Microscope
OBJECTIVES: To know the general parts and the correct techniques of using the microscope.
To learn how to compute the magnifying capacity of the microscope.
MATERIALS: Microscope, glass slide and cover slip.
OBSERVATION: Draw and label this parts of the microscope.
Get the microscope assigned to your group and study its parts. Give
the functions of each part.
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College of Criminology
A. Mechanical Parts
4. Body tube - connects the ocular lens / eyepiece and the objective lenses.
6. Fine Adjustment Knob (FAK) - it brings the specimen into sharp focus.
7. Coarse Adjustment knob (CAK) - it brings the object into approximate focus by raising and
lowering the stage.
9. Inclination Joint - where the microscope arm connects to the microscope base.
B. Optical parts
1. Eyepiece or Ocular - what you look through at the top of the microscope.
4. Oil Immersion Objective – gives the highest magnification, usually 97x or 100x, and is used
wet either with cedar wood oil or synthetic oil.
C. Illuminating parts
2. Grasp the arm with one hand and place the other hand under the base for support.
3. Turn the revolving nosepiece so that the lowest power objective lens is “clicked” into
position.
4. Place the microscope slide on the STAGE and faster it with the stage clips.
6. Look at the slide and lens from the slide when doing this.
7. Look through the eyepiece and ADJUST the light source and diaphragm for the best amount
of light.
8. Slowly turn the coarse adjustment so that the objective lens MOVES away from the slide.
2. Clean smudged lenses with lens paper, and avoid applying pressure with a cloth as the
lenses are very fragile.
3. Wipe the stage ( the platform that holds the slides ) down thoroughly and disinfect the
eyepiece with an alcohol-based wipe.
5. Turn off the light source when the microscope is not in use, this will improve lamp longevity
and save energy.
CONCLUSION:
The objective and ocular lenses are responsible for magnifying the image of
the specimen being viewed.
What is MAGNIFICATION?
The objective should be use are the most common being 4x, 10x, 40x, and 100x, also
known as scanning, low power, high power, and (typically) oil immersion objectives,
respectively.
In microscopy, the objective lenses are the optical elements closest to the specimen. The
objective lens gathers light from the specimen, which is focused to produce the real image that
is seen on the ocular lens.
Objective lenses are the most complex part of the microscope due to their multi-element design.
It is this complexity that makes the objectives the most important components of the device.