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    ICAR ARS NET Previous Questions On Agricultural Biotechnology 2

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    piravin k
    This document contains 40 multiple choice questions related to agricultural biotechnology topics like genetic engineering, plant transformation, and microbial genetics. The questions cover concepts such as genes used for herbicide resistance, restriction enzyme function, Agrobacterium mediated plant transformation process, viral resistance in transgenic plants, and promoters. The answers to each question are also provided.

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    ICAR ARS NET Previous Questions On Agricultural Biotechnology 2

    Uploaded by

    piravin k
    50 views5 pages
    This document contains 40 multiple choice questions related to agricultural biotechnology topics like genetic engineering, plant transformation, and microbial genetics. The questions cover concepts such as genes used for herbicide resistance, restriction enzyme function, Agrobacterium mediated plant transformation process, viral resistance in transgenic plants, and promoters. The answers to each question are also provided.

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    ICAR ARS NET Previous Questions on Agricultural Biotechnology 2

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    This document contains 40 multiple choice questions related to agricultural biotechnology topics like genetic engineering, plant transformation, and microbial genetics. The questions cover concepts such as genes used for herbicide resistance, restriction enzyme function, Agrobacterium mediated plant transformation process, viral resistance in transgenic plants, and promoters. The answers to each question are also provided.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    50 views5 pages

    ICAR ARS NET Previous Questions On Agricultural Biotechnology 2

    Uploaded by

    piravin k
    This document contains 40 multiple choice questions related to agricultural biotechnology topics like genetic engineering, plant transformation, and microbial genetics. The questions cover concepts such as genes used for herbicide resistance, restriction enzyme function, Agrobacterium mediated plant transformation process, viral resistance in transgenic plants, and promoters. The answers to each question are also provided.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 5

    ICAR ARS NET Previous Questions on Agricultural Biotechnology

    21. Endochitinase can be used as an

    a) Antibacterial

    b) Antiviral

    c) Antifungal

    d) Antinematodal

    22. The direct DNA uptake by the protoplast is stimulated by the chemical

    a) Cellulose

    b) Sucrose

    c) PEG

    d) Mannose

    23. Which of the following is an herbicide resistant gene?

    a) Bar

    b) Hpt

    c) Chitinase

    d) oxidase

    24. Restriction enzymes that cuts straight across the DNA produces

    a) Nick

    b) Sticky ends

    c) Blunt ends

    d) None of these

    25. Lambda has short single stranded 5’ projection of

    a) 8 nucleotide length

    b) 10 nucleotide length

    c) 12 nucleotide length

    d) 14 nucleotide length
    26. In Agrobacterium mediated plant transformation which one of the following is used to check the
    Agrobacterium growth

    a) PPT

    b) Kanamycin

    c) Timentin

    d) Pencillin

    27. The T-DNA region of all the Ti and Ri plasmids are flanked by direct repeat sequence

    a) 15bp

    b) 20 bp

    c) 25 bp

    d) 35 bp

    28. During Agrobacterium infections the plant cell begins to synthesise an Arginine derivative called
    as

    a) Hygromycin

    b) Opines

    c) Acetobenzylpurine

    d) Acetosyringone

    29. During Agrobacterium transformation the T-DNA is coated by protein, which is the product of
    gene

    a) Vir B

    b) Vir C

    c) Vir D

    d) Vir E

    30. Most commonly used strategy to develop transgenic plant against any viral disease is to engineer
    a

    a) Anti sense RNA for viral genome

    b) Coat or capsid protein gene

    c) Direct DNA transfer to viral gene


    d) Liposome Mediated transfer of viral RNA

    31. The vir region is organised into six operons viz., vir A, vir B, vir C, vir D, vir E, vir G out of these
    which four operons are required for virulence

    a) A,B,C,D

    b) B,C,D,E

    c) C,D,E,G

    d) A,B,D,G

    32. Replication of DNA by rolling circle mechanism give rise to a

    a) Blunt end DNA

    b) Theta structure

    c) Concatamer

    d) Single strand DNA with nick

    33. . The gene transferred from E.coli to tobacco to increase the level of manitol is

    a) Manitol dehydrogenase

    b) Manitol carboxylase

    c) Manitol oxygenase

    d) Manitol hydrolase

    34. Relaxed plasmid

    a) Have only single copy per cell

    b) Cannot be purified

    c) Does not exist in bacteria

    d) Have multiple copies per cell

    35. Which of the following is a green tissue specific promoter?

    a) Pea vicilin

    b) Arabidopsis small subunit of RUBISCO

    c) 35s CaMV

    d) Bean phytahemaglutinin
    36. In vitro packaging is required for which one of the following vector

    a) BAC

    b) YAC

    c) PAC

    d) None of these

    37. Cosmids have the Cos site of

    a) Lambda

    b) Plasmid

    c) ML3

    d) YAC

    38. Thiobacillus thioxidans grows on

    a) Irron

    b)Sulphur

    c) Aluminium

    d) Pyrites

    39. An example of commercially important primary metabolites is

    a) Toxin

    b) Alkaloid

    c) Amine

    d) Alcohol

    40. Secondary metabolites like antibiotics are not essential for

    a) Log phase growth

    b) Exponential growth

    c) Both a and b

    d) none of these

    Answers:
    21. c) Antifungal
    22. c) PEG
    23. a) Bar
    24. c) Blunt ends
    25. c) 12 nucleotide length
    26. c) Timentin
    27. c) 25 bp
    28. b) Opines
    29. d) Vir E
    30. b) Coat or capsid protein gene
    31. d) A,B,D,G
    32. c) Concatamer
    33. a) Manitol dehydrogenase
    34. d) Have multiple copies per cell
    35. b) Arabidopsis small subunit of RUBISCO
    36. c) PAC
    37. a) Lambda
    38. d) Pyrites
    39. d) Alcohol
    40. c) Both a and b

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