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    Buffers Worksheet and Problem Set

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    This document provides a workbook and problem set on buffers that is due September 15th. It includes definitions of buffers, examples of acid/base reactions in buffered and unbuffered solutions, the Henderson-Hasselbalch equation, and 9 practice problems calculating buffer capacities and concentrations of species in various buffer solutions at different pH levels.

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    Buffers Worksheet and Problem Set

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    369 views3 pages
    This document provides a workbook and problem set on buffers that is due September 15th. It includes definitions of buffers, examples of acid/base reactions in buffered and unbuffered solutions, the Henderson-Hasselbalch equation, and 9 practice problems calculating buffer capacities and concentrations of species in various buffer solutions at different pH levels.

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    This document provides a workbook and problem set on buffers that is due September 15th. It includes definitions of buffers, examples of acid/base reactions in buffered and unbuffered solutions, the Henderson-Hasselbalch equation, and 9 practice problems calculating buffer capacities and concentrations of species in various buffer solutions at different pH levels.

    Copyright:

    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    369 views3 pages

    Buffers Worksheet and Problem Set

    Uploaded by

    Accidentally
    This document provides a workbook and problem set on buffers that is due September 15th. It includes definitions of buffers, examples of acid/base reactions in buffered and unbuffered solutions, the Henderson-Hasselbalch equation, and 9 practice problems calculating buffer capacities and concentrations of species in various buffer solutions at different pH levels.

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    © All Rights Reserved
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    of 3

    Buffers Workbook 

    & Problem Set

    Read Chapter 2.5 and complete the workbook as you go. Complete the problem set
    that follows and show all work for full credit. This Workbook and Problem set is due no
    later than 3AM the night of September 14th (morning of 9/15/12).

    Chapter 2.5:

    Define the term Buffer:

    What happens to the pH of an un-buffered system when acid is added? When Base is
    added?

    Create your own example of these reactions by adding 1 mL of 0.5M HCl and NaOH to
    50 mL of water and calculate the pH.

    Write the Henderson-Hasselbalch Equation:

    Using HA and A- as the acid and base of a buffer, write the reactions for addition of acid
    (H+) and base (OH-).

    Using a pH of 7.0 and a pKa of 6.8 calculate the pH of adding 1 mL of 0.5M HCl and
    NaOH to 50 mL of buffer and calculate the pH.

    Look at the Table 2.8 what buffer would you use to create a buffer solution with a pH of
    8.0 and why?

    Buffers have a capacity. What does this mean?

    If you want to make an acetic acid buffer solution (pKa = 4.76) of 4.5 what would you
    do? Could you calculate exactly how much acid/base you would use to make the
    solution?

    In the book “Andromeda Strain” by Michael Chrichton only two people survive a viral
    attack, a baby with colic and an old man who drinks sterno. How might this be related
    to buffer systems? (Hint: Think about what Colic and the Sterno would do to the body or
    more specifically the blood)

    Problem Set:

    1. Given 0.1 M solutions of acetic acid and sodium acetate, describe the
    preparation of 1L of 0.1 M acetate buffer at a pH of 5.4.
    2. If the internal pH of a muscle cell is 6.8, what is the [HPO42-]/[H2PO4-] ratio in this
    cell?

    3. Given 0.1 M solutions of Na3PO4 and H3PO4, describe the preparation of 1L of a


    phosphate buffer at a pH of 7.5. What are the molar concentrations of the ions in
    the final buffer solution, including Na+ and H+?

    4. BICINE is a compound containing a tertiary amino group whose relevant pKa is


    8.3. Given 1 L of 0.05 M BICINE with its tertiary amino group in the unprotonated
    form, how much 0.1 N HCl must be added to have a BICINE buffer solution of pH
    7.5? What is the molarity of the BICINE in the final buffer?

    5. What are the approximate fractional concentrations of the following phosphate


    species at pH values of 0, 2, 4, 6, 8, 10 and 12?

    a. H3PO4
    b. H2PO4-
    c. HPO42-
    d. PO43-

    6. Citric acid, a tricarboxylic acid important in intermediary metabolism, can be


    symbolized as H3A. Its dissociation reactions are:
    H3A  H+ + H2A- pK1 = 3.13

    H2A-  H+ + HA2- pK1 = 4.76

    HA2-  H+ + A3- pK1 = 6.40

    If the total concentration of the acid and its anion forms is 0.02 M, what are the
    individual concentrations of H3A, H2A-, HA2- and A3- at pH 5.2?

    7. If 50 mL of 0.01 M HCl is added to 100 mL of 0.05 M phosphate buffer at pH 7.2,


    what is the resultant pH? What are the concentrations of H2PO4- and HPO42- in
    this final solution?

    8. If 50 mL of 0.01 M NaOH is added to 100 mL of 0.05 M phosphate buffer at pH


    7.2, what is the resultant pH? What are the concentrations of H2PO4- and HPO42-
    in this final solution?
    9. If the plasma pH is 7.4 and the plasma concentration of HCO3- is 15 mM, what is
    the plasma concentration of H2CO3? What is the plasma concentration of
    CO2(dissolved)? If metabolic activity changes the concentration of CO2(dissolved) to 3
    mM, and [HCO3-] remains at 15 mM, what is the pH of the plasma?

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