Recombinant DNA Technology in Todays Medicine
Recombinant DNA Technology in Todays Medicine
Recombinant DNA Technology in Todays Medicine
ACADEMY
Biology Project
Session : 2021-2022
Submitted by Shaileshwaran.S
Class – XIIth (Science)
Roll no: (cbse) - ______
INDEX
ABSTRACT
GENETIC ENGINEERING
STEPS IN SYNTHESIZING A
RECOMBINANT PROTEIN
GENETIC ENGINEERING HAS PERMITTED
RECOMBINANT DNA TECHNIQUE
PLASMID POLYLINKERS AND MARKER
GENES FOR BLUE WHITE SCREENING
BACTERIAL ARTIFICIAL CHROMOSOMES
AND YEAST ARTIFICIAL CHROMOSOMES
WHY IS A LENTIVIRUS NECESSARY?
REFERENCE
ORISON ACADEMY
Certificate
This is to certify that Shaileshwaran.S student
of class XIIth (Sci.) has successfully prepared
the report on the Project entitled
”Recombinant DNA Technology In Todays
Medicine” under the guidance of Mrs.Dhivya
( Biology).
The report is the result of his efforts & endeavours.
The report is found worthy of acceptance as final
Project report for the subject Biology
of class XIIth (sci.).
Signature of Principal
-----------------------------
Genetic Engineering
Genetic Engineering plays a very important role, not only in scientific research,
but also in the diagnosis and treatment of disease. Recombinant DNA is a tool
in understanding the structure, function, and regulation of genes and their
products.
The objectives of Recombinant DNA technology include:
– Identifying genes
– Isolating genes
– Modifying genes
– Re-expressing genes in other hosts or organisms
→ These steps permit scientists and clinicians to:
– Identify new genes and the proteins they encode
– To correct endogenous genetic defects
– To manufacture large quantities of specific gene products such as hormones,
vaccines, and other biological agents of medical interest
→ Genetic engineering produces proteins that offer advantages over proteins
isolated from other biological sources. These advantages include:
– High purity
– High specific activity
– Steady supply
– Batch-to-batch consistency
→ Once the vector is isolated in large quantities, it can be introduced into the
desired host cells such as mammalian, yeast, or special bacterial cells. The host
cells will then synthesize the foreign protein from the recombinant DNA. When
the cells are grown in vast quantities, the foreign or recombinant protein can
be isolated and purified in large amounts.
→ Recombinant DNA technology is not only an important tool in scientific
research, but has also resulted in enormous progress in the diagnosis and
treatment of certain diseases and genetic disorders in many areas of medicine.
People may be tested for the presence of mutated proteins that may be
involved in the progression of breast cancer, retino-blastoma, and
neurofibromatosis
Scientists are able to link mutations and disease states to specific sites on
chromosomes.
Transferring genes from one organism to another:
People suffering from cystic fibrosis, rheumatoid arthritis, vascular disease, and
certain cancers may now benefit from the progress made in gene therapy.
Lentiviruses can introduce a gene of interest into cells that do not divide –
simple retroviruses cannot.
This ability makes them ideal for a delivery system because most of our cells,
like hemopoietic stem cells, do not divide.