Las Science 7 Melc 2 q2 Week-2
Las Science 7 Melc 2 q2 Week-2
Las Science 7 Melc 2 q2 Week-2
Republic Act 8293, section 176 states that: No copyright shall subsist in any
work of the Government of the Philippines. However, prior approval of the government
agency or office wherein the work is created shall be necessary for exploitation of such
work for profit. Such agency or office may, among other things, impose as a condition
the payment of royalties.
The Science Activity Sheet will help you facilitate the leaching-learning
activities specified in each Most Essential Learning Competency (MELC) with minimal
or no face-to-face encounter between you and learner. This will be made available to
the learners with the references/links to ease the independent learning.
The Science Activity Sheet is developed to help you continue learning even if
you are not in school. This learning material provides you with meaningful and
engaging activities for independent learning. Being an active learner, carefully read
and understand the instructions then perform the activities and answer the
assessments. This will be returned to your facilitator on the agreed schedule.
ii
Name of Learner: ____________________________________________________
Grade and Section: _______________________________Date: _______________
The microscope is a tool, which will help you see tiny living organisms and
objects that cannot be seen by the naked eye by making them look bigger. This
property of the microscope is called its magnifying power or magnification. The
microscope also has the capacity to distinguish small gaps between two separate
points, which humans cannot identify. It is called its resolving power or resolution.
The compound microscope uses diluted light from the sun or artificial light to
illuminate the object to be observed. From its source, the visible light passes through
the small or thin specimen to be observed through the glass lenses. As light passes
through the lenses, it is bent so the specimen appears bigger when it is projected to
the eye. The form and structure of the specimen can then be viewed and seen because
some of their parts reflect light.
Figure 1 below shows the image of the onion skin under the Low Power
Objective (LPO) and High Power Objective (HPO). The use of stains in studying the
onion cells has made cell parts easier to find, observe and identify. Thus, it gives clear
image of the specimen viewed under the microscope.
Figure 1: Image of onion skin under the LPO (left) and HPO (right)
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IV. Activity Proper
Directions: Study the steps in using and proper handling of the compound
microscope, how to make a wet mount, and how to observe specimens in the
succeeding pages of this learning activity sheets and answer the guide questions.
Write your answer on a sheet of paper.
Here are the proper steps to follow in using and proper handling of the compound
microscope, how to make a wet mount and how to observe specimens.
Step 1
After getting the microscope from the box, it should be handled properly by
grasping the curved arm with one hand and supporting the base with the other
hand.
1.a 1.b
The microscope can be It can also be handled by
handled by grasping the arm grasping the arm using the left
using the right with the left hand with the right hand below
hand below the base. the base.
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Step 2
The microscope must be gently placed on the laboratory table with a distance
of at least 10 centimeters from the edge, with its arm facing the one carrying it.
10cm
Step 3
The third step in handling the microscope is to wipe it with the tissue paper or
an old t-shirt.
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B. Steps on How to Make a Wet Mount
2 upwards.
4
Step A medicine dropper is used to add a drop of water to
the specimen as a mounting medium to make the
4 glass slide.
Glass Slide
Step
After positioning the cover slip at 450, the other edge of the
5
Step
The eraser-end of the pencil can be used to gently tap the
cover slip to remove or minimize trapped bubbles. These
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Step 2
The body tube must be carefully lowered until the end of the LPO will almost
touch the cover slip without tilting the microscope.
Step 3
While looking through the eyepiece, the coarse adjustment knob is slowly
turned upward to raise the objective until the letter “e” appears. This step is
continuously done until the letter “e” can be seen clearly. This indicates that
the specimen have already been focused.
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Step 4
To shift LPO to the HPO, the body tube is raised first. While looking from either
side of the microscope, the revolving nosepiece is turned to put the HPO in
place. With the use of the fine adjustment knob the objective lens is slowly
lowered until it almost touches the cover slip.
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Step 5
While looking through the eyepiece, the fine adjustment knob is turned until the
clearest image is seen. Note: There’s a need to raise the objective a little
and look to the side of the microscope while changing objectives.
Guide Questions
1. Based on the image shown in Figures 3 and 4, describe the position of the letter
“e” as seen under the microscope.
2. Compare the image of the letter “e” that you see using your unaided eye (Figure
2) with what you see through the microscope (Figures 3 and 4).
3. Why do you have to watch from the side when changing the objectives?
4. Why is it not good to tilt the microscope while observing a wet slide?
5. Why should the fine adjustment knob be used only when using the HPO?
6. What are the advantages and disadvantages of using the HPO?
7. How important is microscope in studying specimen such as the onion cell?
V. Reflection
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1. The letter “e” is inverted.
2. The image is larger or is enlarged as compared to the one using the eyes only.
With the microscope, the letter “e” also appears grainy and not in straight lines.
3. Objectives are of different lengths. This is done to avoid accidental crashing of
the objectives into the side and breakage of objective lens, glass slide or cover
slip.
4. It is not good to tilt the microscope while viewing a wet mount. It might cause
water to spill and flow into its mechanical parts. This will cause its parts to rust.
5. The HPO is longer and can easily crash into the cover slip and glass slide.
6. The advantage of using the HPO are: the image is greatly magnified, details of
the letter or cell structure can be observed. Disadvantages include: reduced field
of view and the whole letter or structure cannot be observed.
7. The microscope makes one see objects that are not seen by just using the eyes. It
enables one to see enlarged images of specimens such as cells for a thorough
study of their structure and thus, infer their function.
Guide Questions
Key Answer VI.