Multiplication of TMV
Multiplication of TMV
Multiplication of TMV
Physical- i) Wind
ii) Water
Tobacco mosaic virus (TMV) is a single-stranded RNA
virus species that infects a wide range of plants,
especially tobacco and other members of the
family Solanaceae.
2. Uncoating
3. Intracellular Development
4. Assembly (Maturation)
5. Release
TMV can not directly enter the host cell.
It requires damage to plant cells.
It enters through breaches (Gap) in the cell wall.
It is a process in which capsid is removed and nucleic acid is
released into the cell cytoplasm.
Nucleic acid of plant viruses enters the host cell cytoplasm
along with capsid.
In the cytoplasm capsid is removed and nucleic acid is freed.
It requires assessment of host enzymes to remove capsids.
In order to produce disease, the virus must replicate
and spread to neighbouring cells and then
systematically throughout the plant.
Spreads of virus to neighbouring cells occurs through
microscopic channels in the cell walls called
plasmodesmata.
Plasmodesmata-
These are slender structures
extending from cell wall
which connects adjacent
plant cells.
Spread to other parts of the plant by vasculature system
(Xylem and phloem).
The cell-to-cell movement of virus requires one or more
protein called as “Movement protein”.
Most plant viruses use coat/capsid proteins for transfer.
TMV contains SS Positive sense RNA as its genome.
Replication of virion RNA thus involves synthesis of negative
strand RNA using positive strand RNA as a template.
Thus, replication completes in 2 steps-
1) Synthesis of negative strand RNA using positive strand RNA
as a template which forms doubles stranded intermediate
termed as “Replicative form (RF)”.
2) Synthesis of positive strand RNA using negative strand
RNA as a template using virus coded RNA dependent RNA
polymerase.
Assembly