CBCS: 2019 Pattern S. Y. B. Sc.

Microbiology

Savitribai Phule Pune University

(Formerly University of Pune)

Three Year B.Sc. Degree Program in Microbiology

(Faculty of Science & Technology)

S. Y. B. Sc. (Microbiology)

Choice Based Credit System Syllabus

2019 Pattern

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CBCS: 2019 Pattern S. Y. B. Sc. Microbiology

Savitribai Phule Pune University, Pune-411007

Syllabus 2020-21
S. Y. B. Sc. Microbiology Semester III and Semester IV
Titles of the Papers

Semester Paper Code Paper Paper title

MB 231 I Medical Microbiology and Immunology

III MB 232 II Bacterial Physiology and Fermentation Technology

MB 233 III Practical based on MB 231 and MB 232

MB 241 I Bacterial Genetics

IV MB 242 II Air, Water and Soil Microbiology

MB 243 III Practical based on MB 241 and MB 242

Equivalence of Previous Syllabus: S. Y. B. Sc. Microbiology

Semester Old version (2013 Pattern) New Syllabus (2019 Pattern)

implemented from 2014-15 implemented from 2020-21
Course Course title Course Course title
Number Number
MB: 212 Industrial and Soil MB 231 Medical Microbiology and
Microbiology Immunology
III MB: 211 Bacterial Systematics and MB 232 Bacterial Physiology and
Physiology Fermentation Technology
MB: 221 Bacterial Genetics MB 241 Bacterial Genetics

MB: 222 Air and Water Microbiology MB 242 Air, Water and Soil Microbiology
IV

MB: 223 Practical Course based on MB 233 Practical based on Theory papers
Annual MB:211, MB:212, MB:221, MB 231 and MB 232
Practical MB:222 MB 243 Practical based on Theory papers
MB 241 and MB 242

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CBCS: 2019 Pattern S. Y. B. Sc. Microbiology
Semester III
DSEC-MB-231: Medical Microbiology and Immunology
[2 Credits; 36 Lectures]
[1 credit=15 hrs x 60 mins 900mins/50mins= 18 lectures]
Course Outcomes:
 Understanding the concept of epidemiology with respect to terms like Incubation period, Viability,
Susceptibility, Pathogenicity, Virulence, Pathogenesis, Lab diagnosis, Epidemic, Sporadic,
Endemic and Pandemic.
 Acquainted with human pathogens such as Escherichia coli, Staphylococcus aureus and Fungi like
Yeast- Candida as well as Dermatophytes.
 Principles of Chemotherapy are introduced based on Selective toxicity, Bioavailability, MIC,
MBC, LD50. Accustomed with the terms Antagonism and synergism in drug administration.,
Antibiotic sensitivity, Antibiotic misuse/antibiotic overuse and Concept of drug resistance
(e.g., MRSA, ESBL)
 Comprehend the term immunity with its types
 Get knowledge of haematopoiesis, Antigens and antibodies, Immunohematology, Inheritance of
ABH antigens, Medico legal applications of blood groups
 Acquainted with Active and Passive immunization

MB–231 Medial Microbiology and Immunology [36]

Credit I Medical Microbiology (18)

1 Definitions 2
Incubation period, Viability, Susceptibility, Pathogenicity,
Virulence, Pathogenesis, Lab diagnosis, Epidemic, Sporadic,
Endemic, Pandemic
2 Study of following pathogens with respect to 10
Classification, Morphological, Cultural and Biochemical
characters, Antigenic structure, Viability characteristics,
Pathogenicity, Pathogenesis, Symptoms, Laboratory diagnosis,
Epidemiology, Prophylaxis and Chemotherapy:
Bacteria: a) Escherichia coli b) Staphylococcus aureus
Fungi: a) Candida b) Dermatophytes

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CBCS: 2019 Pattern S. Y. B. Sc. Microbiology
3 Introduction to Chemotherapy 6
i. Selective toxicity, Bioavailability, MIC, MBC, LD50
ii. Antagonism and synergism in drug administration
iii. Antibiotic sensitivity
iv. Antibiotic misuse/antibiotic overuse
v. Concept of drug resistance (e.g. MRSA, ESBL)

Credit II Immunology (18)

4 Immunity 2
Definition, Types (Innate and acquired, active and passive, humoral
and cell mediated)
5 Formation of blood cells (Hematopoiesis) 4
Myeloid and lymphoid lineages and differentiation process
Lymphocyte types
6 Antigens and antibodies: Definition and Concept 2
7 Immunohematology 7
i. ABO and Rh blood group systems
ii. Bombay blood group
iii. Biochemistry of blood group substances
iv. Inheritance of ABH antigens
v. Medico legal applications of blood groups

8 Active and Passive Immunization 3

i. Active Immunization -Whole organism vaccines
a) Attenuated vaccines
b) Inactivated Vaccines
ii. Passive Immunization
Transfer of preformed antibodies
iii. Latest Immunization schedule in India

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 CBCS: 2019 Pattern S. Y. B. Sc. Microbiology

References:
1. Kanungo Reba. (2017). Ananthanarayan and Paniker's Textbook of Microbiology.
Tenth edition. The Orient Blackswan Publisher. ISBN-13: 978-9386235251
2. Collins C. H., Lyne P. M., Grange J. M. and Falkinham J. O. III. (Editors). (2004).
Collins and Lyne's Microbiological Methods. 8th edition. Arnold, London; Oxford
3. Finch R., Greenwood D., Whitley R. and Norrby S. R. (2010) Antibiotic and
Chemotherapy. 9th Edition. Elsevier. ISBN: 9780702040641
4. Dulbecco R., Eisen H. N. and Davis B. D. (1990). Microbiology. United States:
Publisher -Lippincott. ISBN: 9780608072432
5. Dey N. C., Dey T. K. and Sinha D. (2013). Medical Bacteriology Including Medical
Mycology and AIDS. 17th Edition. New Central Book Agency (P) Ltd (Publisher). India
6. Kindt T. J., Goldsby R. A. and Osborne B. A. (2007). Kuby Immunology. 6th Ed.
W. H. Freeman and Co., New York.
7. MacFaddin J. F. (1985). Media for Isolation-Cultivation-Identification –Maintenance of
Medical Bacteria. Vol. I. Williams and Wilkins, Baltimore
8. MacFaddin J. F. (2000). Oxidation- Fermentation Test. Biochemical Tests for Identification of
Medical Bacteria. 3rd ed. Philadelphia: Lippincott Wilkins and Williams. 379-387. B, III
9. Mukherjee K. L. and Ghosh S. (2010). Medical Laboratory Technology, Volume III: Procedure
Manual for Routine Diagnostic Tests. 2nd edition. McGraw Hill Education (India) Private
Limited. ISBN-13: 978-1259061257
10. Mukred A. M., Hamid A. A., Hamzah A. and Wan Mohtar Wan Yusoff W. M. W. (2008).
Growth Enhancement of Effective Microorganisms for Bioremediation of Crude Oil
Contaminated Waters. Pakistan Journal of Biological Sciences.11: 1708-1712.
11. Pathak S. S. and Palan V. (1997). Immunology-Essential and Fundamental, Pareen Publications
Bombay.
12. Public Health England. (2019). Oxidation/fermentation of glucose test. UK Standards for
Microbiology Investigations. TP 27 Issue 4. https://www.gov.uk/uk-standards-
formicrobiology-investigations-smi-quality-and-consistency-in-clinical-laboratories
13. Roitt Evan, Brostoff J., Male D. (1993) Immunology. 6th Edition. Mosby and Co. London.
14. Roitt I. M. (1988). Essentials of Immunology, ELBS, London.
15. Roitt M. (1984). Essentials of Immunology. P. G. Publishers Private Limited, New Delhi.
16. Roth J. A., Bolin C., Brogden K. A., Chris Minion K. F. and Wannemuehler M. J. (1995).
Virulence mechanisms of bacterial pathogens. Second edition. American Society for
Microbiology. ISBN-13: 978-1555810856
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CBCS: 2019 Pattern S. Y. B. Sc. Microbiology
17. Schuenke S. (1997). Medical Microbiology. Fourth edition. University of Texas Medical
Branch of Galvesion. Samuel Baron (Editor). ASIN: B008UYPLIO
18. Champoux J. J., Neidhardt F. C., Drew W. L. and Plorde J. J. (2004). Sherris Medical
Microbiology: An Introduction to infectious diseases. 4th edition. Ryan K. J. and Ray C.
19. G. (Editors). McGraw-Hill Companies. DOI: 10.1036/0838585299
20. Shrivastava M., Navaid S., Peethambarakshan A., Agrawal K. and Khan A. (2015). Detection
of rare blood group, Bombay (Oh) phenotype patients and management by acute normovolemic
hemodilution. Asian journal of transfusion science. 9(1):74–77
21. Standards Unit. National Infection Service. PHE Bacteriology – Test Procedures.TP. 27(4):
Issue date: 16.01.19. 1-14
22. Stites D. P., Stobo J. D., Fudenberg H. H. and Wells J. V. (1982). Basic and Clinical
Immunology. 4th Edition. Lange Medical Publications, Maruzen Asia Pvt. Ltd., Singapore.
23. Gangal S. and Sontakke S. (2013). Textbook of Basic and Clinical Immunology. Universities
Press Private limited. Available also at Orientblackswan, India. ISBN: 9788173718298
24. Talwar G. P. (1983). Handbook of Immunology. Vikas Publishing Pvt. Ltd. NewDelhi.
25. Paul W. E. (2003): Fundamental Immunology. 5th edition. Lippincott Williams and Wilkins
Publishers. ISBN: 9780781735148
26. Joklik W. K., Willett H. P., Amos D. B. and Wilfert C. M. 1995). Zinsser's Microbiology. 20th
Edition. Appleton and Lange Publisher. ISBN-13: 978- 0838599839
27. Zajic J. E. and Supplisson B. (1972). Emulsification and degradation of “Bunker C” fuel oil by
microorganisms. Biotechnol. Bioeng. 14: 331-343.

Savitribai Phule Pune University (SPPU), Pune 6 |22

CBCS: 2019 Pattern S. Y. B. Sc. Microbiology

MB-232: Bacterial Physiology and Fermentation Technology

[2 Credits; 36 Lectures]
[1 credit=15 hrs x 60 mins = 900mins/50mins= 18 lectures]
Course Outcomes:
 Acquainted with the term Enzymes, its nomenclature and classification and models for catalysis
 Understand the effect of pH, temperature, substrate concentration, enzyme concentration,
activators and inhibitors on enzymes
 Understanding the concept of Bacterial Physiology with reference to metabolism, catabolism,
anabolism, respiration and fermentation
 Comprehend the different metabolic pathways with structures
 Acquainted with design of a fermenter, fermentation parameters, use of media for industrial
fermentations
 Understand the sources of contamination during fermentations
Credit I Bacterial Physiology (18)

Enzymes
i. Introduction to Enzymes: Properties of enzymes, Nature of active 2
site, Structure of active site, commonly occurring amino acids at
active site. Ribozymes, coenzymes, apoenzymes, prosthetic group
and cofactors.
ii. Nomenclature and classification as per IUB (up to class level). 2
1 iii. Models for catalysis– 1
a) Lock and key
b) Induced fit
c) Transition state.
iv. Effect of pH and temperature, substrate concentration and enzyme 3
concentration, activators and inhibitors of enzyme
Bacterial Physiology
i. Definitions of Metabolism, catabolism, anabolism, respiration and 1
fermentation

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CBCS: 2019 Pattern S. Y. B. Sc. Microbiology
ii. Metabolic pathways (with structures)
a) Embden-Meyerhof-Parnas pathway (Glycolysis) 2
b) Hexose monophosphate pathway 2
2 c) Entner-Doudoroff pathway 1
d) Phosphoketolase pathway (Pentose and hexose) 1
e) TCA cycle (with emphasis on amphibolism) and Glyoxylate bypass 2
f) Gluconeogenesis and its significance 1

Credit II Fermentation Technology (18)

3 Concept of fermentation technology 4
i. Microbial biomass- based fermentation (Biofertilizer,
biopesticide and Probiotics)
ii. Production of Primary metabolites (Organic acids, amino acids,
vitamins and enzymes)
iii. Production of Secondary metabolites (Antibiotics)
iv. Production of recombinant products (insulin and growth
hormones)
v. Production of Fermented food products (Cheese, yoghurt)
vi. Microbial biotransformation (Steroid transformation)
4 Strains of industrially important microorganisms: 5
i. Desirable characteristics of industrial strain
ii. Principles and methods of primary and secondary screening
iii. Master, working and seed culture; development of inoculum
iv. Preservation and maintenance of industrial strains.

5 Design of a Fermenter (typical CSTR Continuous stirred 2

Tank Reactor): Different parts and their working

6 Monitoring of different fermentation parameters 2

(Temperature, pH, aeration, agitation, foam)
7 Types of fermentations: Batch, continuous and dual 2
8 Media for industrial fermentations: 2
Constituents of media (Carbon source, nitrogen source, amino acids,
vitamins, minerals, water, buffers, antifoam agents, precursors, inhibitors
and inducers)
9 Contamination: Sources, precautions and consequences 1

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CBCS: 2019 Pattern S. Y. B. Sc. Microbiology

References:
a. BIOTOL Series. (1993). Biotechnology by open learning series. Defense
Mechanisms.Butterworth and Heinemann Ltd., Oxford
b. Casida L. E. J. R. (2016). Industrial Microbiology. New Age International Private
Limited. ISBN- 9788122438024

c. Conn E. E., Stumpf P. K., Bruening G., Doi R. Y. (1987). Outlines of

Biochemistry. 5th Edition, John Wiley and Sons, New Delhi. (Unit I& II)
d. Madigan M. T., Martinko J. M. and Brock T. D. (2006). Brock’s Biology of
Microorganisms. Pearson Prentice Hall, Upper Saddle River.
e. Moat A. G. and Foster J. W. (1988). Microbial Physiology. 2nd Edition. John
Wiley and Sons New York.
f. Nelson D. L. and Cox M. M. (2005). Lehninger’s Principles of Biochemistry.8 th edition.
Mac Millan Worth Pub. Co. New Delhi. ISBN:9781319228002
g. Patel A. H. (2016). Industrial Microbiology. Trinity Press (Publisher).
ISBN-13- 9789385750267
h. Peppler H. L. and Perlman D. (1979). Microbial Technology. Volume 1:
Microbial Processes. Academic Press, New York. ISBN: 978-0-12-551501
i. Peppler H. L. and Perlman D. (1979). Microbial Technology. Volume II:
Fermentation Technology (2nd Edition). Academic Press. ISBN: 9781483268279
j.
Prescott L. M., Harley J. P. and Klein D. A. (2005). Microbiology. 6th
Edition. Mac Graw Hill Companies Inc. (Unit II)
k. Reed G. (Editor). (1982). Prescott and Dunn’s Industrial Microbiology. Westport,
CT, AVI Publishing Co Inc.
l. Stanbury P. F., Whitaker A. and Hall S. J. (2016). Principles of Fermentation
Technology.3rd Edition. Butterworth-Heinemann. ISBN: 9780080999531
m. Voet D. and Voet J. G. (1995). Biochemistry. 2nd Edition. John Wiley &
sons. New York. ISBN 0-471-58651-X

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CBCS: 2019 Pattern S. Y. B. Sc. Microbiology
MB-233: Practical Course based on
MB-231: Diagnostic Microbiology and Immunology
and
MB-232: Bacterial Physiology and Fermentation Technology
[2 Credits: 78 Lectures]
[1 credit=15hrs x 130 mins = 1950 mins/50 mins=39 lectures]
78 L distributed as 60 L for performing practicals and 18 L for internal evaluation
12 Practicals x 5 lectures = 60 Lectures

Semester III: Practical course based on MB 231and MB 232

Expt. Topics No. of

No. Practicals
1 Measurements of cell dimension by micrometry using 10x, 45x and 100x 1
objectives
2 Staining techniques: Cell Wall Staining 1

3 Blood grouping: ABO, Rh and Bombay blood group (anti H Lectin test) 1

4 Isolation and identification of pathogens from clinical samples: 6

(Escherichia coli, Staphylococcus aureus and Proteus) by
a. Gram staining & motility,

b. Cultural and Biochemical characteristics

i. Sugar utilization test,

ii. Sugar fermentation test,

iii. Triple Sugar iron agar,

iv. IMViC test

v. Enzyme detection – Gelatinase, Catalase, Oxidase,

Coagulase (free and bound)

vi. Oxidative-fermentative test [Baird Parker’s modification

of Hugh and Leifson’s oxidative- fermentative (OF) basal
medium for Gram Positive and Hugh and Leifson’s
oxidative- fermentative (OF) basal medium for Gram
negative; Public Health England, 2019]

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CBCS: 2019 Pattern S. Y. B. Sc. Microbiology
5 Primary screening of industrially important organisms: 2

a. Screening and isolation of antibiotic and organic acid producing

organism from soil by Crowded plate and Giant colony method

b. Microorganisms producing industrially important enzyme-amylase 1

Total 12

References:
Experiment 1. Measurements of cell dimension by micrometry:
1. Dubey R. C. and Maheshwari D. K. (2002). Practical Microbiology. S. Chand and Company
Limited, New Delhi, India
2. Gunasekaran P. (2007). Laboratory Manual In Microbiology. New Age International (P)
Limited New Delhi, India
3. Muskan K. and Patil U. K. (2009). Essentials of Biotechnology. I. K. International Publishing
House Private Limited, New Delhi, India.
4. Saxena J., Baunthiyal M. and Ravi I. (2015). Laboratory Manual of Microbiology,
Biochemistry and Molecular Biology. Scientific Publishers, New Delhi, India

Experiment 2. Cell Wall Staining

1. Saxena J., Baunthiyal M. and Ravi I. (2015). Laboratory Manual of Microbiology, Biochemistry
and Molecular Biology. Scientific Publishers, New Delhi, Indi
Experiment 3. Blood grouping:
2. Godkar D. P. (2003). Textbook of Medical Laboratory Technology. Bhalani
Publishing House, New Delhi, India.
3. Mukherjee K. L. (2013). Medical Laboratory Technology. Second Edtion. Volume III.
McGraw-Hill Companies, India.
Experiment 4. Isolation and identification of pathogens from clinical samples:
1. Mac Faddin J. F. (2000). Biochemical Tests for Identification of Medical Bacteria.
United
2. Randhawa V. S., Mehta G. and Sharma K. B. (2009). Practicals and Viva in Medical
Microbiology. Second Edition. Elsevier (A Division of Reed Elsevier India Pvt.
Limited).
3. Verhaegen J. and Heuck C. C .(Editors). (2003). Basic Laboratory Procedures in
Clinical Bacteriology. Second Edition. Switzerland: World Health Organization.

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CBCS: 2019 Pattern S. Y. B. Sc. Microbiology
Experiment 4. b.i. Sugar utilization test: Minimal salt Medium (MSM with 1%
sugar):
1. Mukred A. M., Hamid A. A., Hamzah A. and Wan Yusoff W. M. (2008). Enhancement of
Biodegradation of Crude Petroleum-Oil in Contaminated Water by the Addition of Nitrogen
Sources. Pakistan Journal of Biological Sciences, 11: 2122-2127.
2. Mahalingam B. L., Karuppan M. and Manickam V. (2013). Optimization of Minimal Salt
Medium for Efficient Phenanthrene Biodegradation by Mycoplana sp. MVMB2 Isolated
from Petroleum Contaminated Soil Using Factorial Design Experiments. CLEAN - Soil, Air,
Water. 41(1): 51–59. Wiley-VCH Verlag GmbH and Co. KGaA, Weinheim
Experiment 4. b. ii. Sugar fermentation test:- Phenol Red Broth Base:
1. Aneja K. R. (2007). Experiments in Microbiology, Plant Pathology and Biotechnology. New Age
International, New Delhi, India
2. Dubey R. C. and Maheshwari D. K. (2002). Practical Microbiology. S. Chand and Company
Limited, New Delhi, India
3. Mac Faddin J. F. (2000). Biochemical Tests for Identification of Medical Bacteria. United
Kingdom: Lippincott Williams and Wilkins.
Experiment 4. b. iii. Triple sugar Iron Agar:
1. Jain A., Agarwal J. and Venkatesh V. (2018). Microbiology Practical Manual. 1st Edition. E-
Book. Elsevier Health Sciences, India.
2. Mac Faddin J. F.(2000). Biochemical Tests for Identification of Medical Bacteria.United
Kingdom:Lippincott Williams and Wilkins.
3. Randhawa V. S., Mehta G. and Sharma K. B. (2009). Practicals and Viva in Medical
Microbiology. Second Edition. Elsevier (A Division of Reed Elsevier India Pvt. Limited).
Experiment 4. b. iv. IMViC test:
1. Dubey R. C. and Maheshwari D. K. (2002). Practical Microbiology. S. Chand and Company
Limited, New Delhi, India
2. Jain A., Agarwal J. and Venkatesh V. (2018). Microbiology Practical Manual. 1st Edition. E-
Book. Elsevier Health Sciences, India.
3. Randhawa V. S., Mehta G. and Sharma K. B. (2009). Practicals and Viva in Medical
Microbiology. Second Edition. Elsevier (A Division of Reed Elsevier India Pvt. Limited).
4. Verma A. S., Das S., and Singh A. (2014). Laboratory Manual for Biotechnology. S Chand
and Company Limited, New Delhi, India
Experiment 4. b. v. Enzyme detection:
1. Carroll K.C., Pfaller M. A., Landry M. L., McAdam A. J., Patel R., Richter S. S. and Warnock
D. W. (Editors). (2019). Manual of Clinical Microbiology. 2 Volume Set. 12th Edition. John
Savitribai Phule Pune University (SPPU), Pune 11 | 2 2
CBCS: 2019 Pattern S. Y. B. Sc. Microbiology
Wiley, USA
2. Dubey R. C. and Maheshwari D. K. (2002). Practical Microbiology. S. Chand and Company
Limited, New Delhi, India
3. Goldman E. and Green L. H. (2008). Practical Handbook of Microbiology. United States: CRC
Press.
4. Leber A. L. (2020). Clinical Microbiology Procedures Handbook. United States: Wiley.
5. Verhaegen J. and Heuck C. C . (Editors). (2003). Basic Laboratory Procedures in Clinical
Bacteriology. Second Edition. Switzerland:World Health Organization.
Experiment 5. Primary screening of industrially important organisms:
1. Aneja K. R. (2007). Experiments in Microbiology, Plant Pathology and Biotechnology. New
Age International, New Delhi, India
2. Dubey R. C. and Maheshwari D. K. (2002). Practical Microbiology. S. Chand and Company
Limited, New Delhi, India
3. Gunasekaran P. (2007). Laboratory Manual in Microbiology. New Age International Private
Limited, New Delhi, India.

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 CBCS: 2019 Pattern S. Y. B. Sc. Microbiology
S. Y. B. Sc. Microbiology Syllabus (Semester IV)
MB-241: Bacterial Genetics
[2 Credits; 36 Lectures]
[1 credit=15 hrs x 60 mins = 900mins/50mins= 18 lectures]

Course Outcomes:
 Understanding the different experimental evidence for nucleic acid as genetic material
 Comprehend the different types of nucleic acids, Structure of DNA and Prokaryotic DNA replication.
 Understand the different models and modes of DNA replication with its basic rules of DNA replication
 Get knowledge of Gene expressions, Mutations and reversions
 Acquainted with Plasmid genetics

MB 241 Bacterial Genetics [36]

Credit I Topics (18)

Understanding DNA: 7
i. Experimental evidence for nucleic acid as genetic material.
1
a. Discovery of transforming material (hereditary material):
b. Griffith’s experiment
c. Avery and MacLeod experiment
d. Gierer and Schramm
e. Fraenkel-Conrat and Singer experiment (TMV virus)
f. Hershay and Chase experiment

ii. Types of nucleic acids (DNA and RNAs) 1

iii. Structure of DNA 2

a. Structure of Nitrogen bases, Nucleoside, Nucleotide and
polynucleotide chain
b. Bonds involved in DNA structure
c. Different forms of DNA
iv. Prokaryotic DNA replication 8
2
a. Models of DNA replication (Conservative, semi-
conservative and Dispersive)
b. Meselson and Stahl’s experiment (semi-conservative)
c. Six basic rules of DNA replication
d. Enzymes, proteins and other factors involved
in DNA replication.
e. Modes of DNA replication Rolling circle mechanism, theta
and linear DNA replication

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CBCS: 2019 Pattern S. Y. B. Sc. Microbiology
Credit II Topics (18)

i. Gene expression 2
3 a. Concept of Genetic code and its properties
b. Concept of transcription and translation
ii. Mutations and reversions 10
Concept of Mutation and Types of mutations: Nonsense,
Missense, Silent, Conditional lethal-temperature sensitive,
Amber, Reverse, suppressor

a. Spontaneous Mutation

 Discovery of spontaneous mutation (Fluctuation test)

 Mechanism of spontaneous mutation
4
 Isolation of Mutants: Replica plate technique
b. Concept of Induced Mutations
 Base pair substitution (Transitions, Transversions),
Insertions and deletions-Frame / Phase shift mutations
 Physical Mutagenic agent: UV and X-ray
 Chemical mutagenic agents
 Base analogues (2 amino purine, 5 bromouracil),
 HNO2, Alkylating agents
 Intercalating agents (EtBr, acridine orange)

iii. Plasmid genetics 6

a. Types of plasmids
b. Properties of Plasmid
c. Plasmid replication
5
d. Plasmid incompatibility
e. Plasmid curing
f. Plasmid amplification Concept

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CBCS: 2019 Pattern S. Y. B. Sc. Microbiology

References:
1. Brooker R. J. (2012). Genetics: Analysis and Principles. 4 th edition. McGraw- Hill-
Publication
2. Alberts B., Johnson A., Lewis J., Raff M., Roberts K. and Walter P. (2008). Molecular
Biology of the Cell. 5th Edition. Garland Science. Taylor and Francis. ISBN: 978-0-8153-
4105-5. .
3. Malacinski G. M. (2005). Freidfelder’s Essentials of Molecular Biology. 4th Edition. Jones
and Bartlett Publishers, Inc
4. Gardner E. J., Simmons M. J. and Snustad D. P. (2006). Principles of Genetics. 8 th edition.
John Wiley and Sons Publication. ISBN-13: 9788126510436
5. Hayes W. (2nd Edition). (1968). Genetics of Bacteria and their Viruses. Oxford-Edinburgh:
Blackwcll Scientific Publications.
6. Watson J.D., Baker, T.A., Bell, S.P., Gann A., Levine M. and Losick R. (2014). Molecular
Biology of the gene. 7th edition. Pearson. ISBN: 9780321762436
7. Krebs J., Kilpatrick S. T., Goldstein E. S. (Editors). Lewin's GENES X (2011). 10 th
Edition.Sudbury, Mass: Jones and Bartlett, c2011
8. Lodish H., Berk A., Kaiser C. A., Krieger M., Bretscher A., Ploegh H., Martin K. C., Yaffe
M. and Amon A. (2021). Molecular Cell Biology, 9th Edn. Macmillan Learning.ISBN:
9781319208523
9. Pawar and Daginawala. General Microbiology. Vol. I and vol II. 1st Edition.
Himalaya Publishing House, Mumbai
10. Primrose S. B. (2002) .Principles of Gene Manipulation. 6 th Edition. Oxford: Blackwell
Scientific Publications
11. Russel P. J. (2000). Fundamentals of Genetics. Publisher:
Benjamin/Cummings. ISBN:9780321036261
12. Russel P. J. (2010). iGenetics: A Molecular Approach. 3rd Edition. Benjamin Cummings.
ISBN: 9780321569769
13. Stanier R. Y. (2003). General Microbiology. United Kingdom: Palgrave Macmillan
Limited.
14. Strickberger M. W. (2012). Genetics. 3rdEdition. New Delhi: PHI Learning Gardner

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 CBCS: 2019 Pattern S. Y. B. Sc. Microbiology
S. Y. B. Sc. Microbiology Syllabus (Semester IV)
MB-242: Air, Water and Soil Microbiology
[2 Credits; 36 Lectures]
[1 credit=15 hrs x 60 mins = 900mins/50mins= 18 lectures]
Course Outcomes:
 The course will help them to get knowledge of the Air Microbiology, methods of air sampling,
different types of air samplers, air sanitation and airborne infections.
 Deals with water microbiology including bacteriological analysis of water, methods of water
purification, water borne infections and bacteriological standards of water quality.
 Understand Soil Microbiology, rhizosphere, composting and humus formation, biofertilizers,
biocontrol agents and microbial interactions.
 Acquire knowledge of carbon and nitrogen cycles with role of microorganisms.

MB-242 Air, Water and Soil Microbiology [36]

Credit I Air Microbiology and Water Microbiology 18

i. Air Microbiology

a. Air flora 1
 Transient nature of air flora
 Droplet, droplet nuclei and aerosols
b. Methods of Air sampling and types of air samplers
1  Impaction on solids
 Impingement in liquid
 Sedimentation 3

 Centrifugation
c. Air sanitation: Physical and chemical methods 2

d. Airborne infections 1
ii. Water Microbiology

a. Types of water: surface, ground, stored, distilled, mineral and 1

de-mineralized water

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CBCS: 2019 Pattern S. Y. B. Sc. Microbiology
b. Recommended Bacteriological standards of Water Quality
 Maharashtra Pollution Control Board

2 (MPCB) Main Functions of MPCB

Water quality standards for best designated usages 1
 Central Pollution Control Board
(CPCB) Main Functions of CPCB
Designated Best Use Water Quality Criteria
c. Water purification methods 2
d. Water borne Infections 1
e. Indicators of faecal pollution: 2
Escherichia coli, Bifidobacterium, Streptococcus faecalis,
Clostridium perfringens,
New indicators: Campylobacter and Pseudomonas
f. Bacteriological analysis of water for potability 4
i. Bacteriological standards of potable water: Bureau of
Indian standards (BIS)
ii. World Health Organization (WHO)
iii. Presumptive coliform count
iv. Confirmed test
v. Completed test
vi. Eijkman test
vii. Membrane filter technique
Credit Soil Microbiology 18
II a. Rhizosphere microflora and its role in the rhizosphere 1

b. Role of microorganisms in composting and humus formation 2

c. Biofertilizers: Bacterial, Cyanobacterial, fungal and their large- 3

scale production
d. Biocontrol agents: Bacterial, Viral, Fungal and their large- 3
scale production
e. Brief account of microbial interactions: 5
Symbiosis, Neutralism, Commensalism, Competition, Ammensalism,
Synergism, Parasitism and Predation

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 CBCS: 2019 Pattern S. Y. B. Sc. Microbiology
f. Role of microorganisms in elemental cycles in nature: Carbon, 4
Nitrogen

References:
1. Aithal S. C. and Kulkarni N. S. (2015). Water microbiology ~ an Indian perspective.
Published by Himalaya Publishing House, IstEdition. ISBN: No.: 978-93-5202-129-1.
2. Dube H. C. and Bilgrami K. S. (1976). Textbook of modern pathology. Vikas publishing
house. New Delhi.
3. Dubey R. C. and Maheswari D.K. Textbook of Microbiology. S. Chand Publishing. ISBN:
9788121926201

4. Frobisher M. (1974). Fundamentals of Microbiology. 9th Edition. Saunders, Michigan

University Press. ISBN: 9780721639222
5. Ingraham C. A. and Ingraham J. L. (2000). Introduction to Microbiology. United Kingdom:
Brooks/Cole.
6. Lim D. V. (1989). Microbiology. 2nd Edition. West Publishing Company. ISBN:
9780314262066
7. Madigan M. T., Thomas Brock T., Martinko J., Clark D. P. and Paul D. P. (2009). Brock’s
Biology of Microorganisms. Pearson/Benjamin Cummings. ISBN: 9780132324601
8. Martin A. (1977). An Introduction to Soil Microbiology. 2nd edition. John Wiley & Sons Inc.
NewYork & London.
9. Martin A. Introduction to Soil Microbiology. (1961). John Wiley &Sons, New York and
London publication
10. MPCB, CPCB, BIS and WHO websites guidelines for drinking water quality
11. Pawar C. B. and Daginawala H.F. (1982). General Microbiology. Vol. I and II. 1 st Edition.
Himalaya Publishing House, Mumbai. ISBN: 9789350240892and ISBN9789350240908
12. Pelzar M. J., Chan E. C. S. and KriegN. R. (1986). Microbiology. 5th Edition. McGraw-Hill
Publication
13. Prescott L. M., Harley J. P. and Klein D. A. (2006). Microbiology. 6 th Edition. McGraw Hill
Higher Education. ISBN-13: 978-0-07-295175-2
14. Rangaswami G. (1979) Recent advances in biological nitrogen fixation. Oxford and IBH.
New Delhi.
15. Salle A. J. (1971). Fundamental Principles of Bacteriology.7th Edition. Tata MacGraw
Publishing Co.
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CBCS: 2019 Pattern S. Y. B. Sc. Microbiology
16. Schlegel H. G. (1993). General Microbiology. 8thEdition. Cambridge University Press
17. Stanier R. Y. (2003). General Microbiology. United Kingdom: Palgrave Macmillan
Limited.
18. Subba Rao N. S. (1977). Soil Microbiology. 4thEdition. Oxford and IBH Publishing Co. Pvt.
Ltd.
19. Tortora G. J., Funke B. R. and Case C. L. (2016). Microbiology: An introduction 12th
Edition, Pearson. ISBN-13: 9780321929150

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 CBCS: 2019 Pattern S. Y. B. Sc. Microbiology

S.Y. B. Sc. Microbiology Syllabus (Semester IV)

MB-243: Practical Course based on
MB-241: Bacterial Genetics and MB-242: Air, Water and Soil Microbiology
[2 Credits: 78 Lectures]
[1 credit=15hrs x 130 mins = 1950 mins/50 mins=39 lectures]
78 L distributed as 60 L for performing practicals and 18 L for internal evaluation
12 Practicals x 5 lectures = 60 Lectures
Semester IV: MB-243: Practical course based on MB 241and MB 242
Expt. Topics No. of
No. Practicals
1. Staining Techniques: 2
i. Flagella Staining ii. Metachromatic Granules
2. Air sampling using an air sampler, calculation of air flora from 1
different locations with the knowledge of respective standards of
bacterial and fungal counts.
3. Air Flora: 1
a. Diversity determination.
b. Simpson index and settling velocity determination
4. Bacteriological tests for potability of water 3
a. MPN, Confirmed and Completed test.
b. Membrane filter technique (Demonstration)
5. i. UV- survival curve 2
ii. Induction of mutation by using physical mutagen
(e.g. U V rays)
iii. Isolation of auxotrophic mutants by Replica Plate
Technique
6. Enrichment, Isolation, Preparation and Application of 2
Bioinoculants
i. a) Azotobacter species and b) Rhizobium species
Or
ii. Blue Green Algae (Cyanobacteria)
7. Visit to Industry/ Drinking Water treatment plant 1
Total 12

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CBCS: 2019 Pattern S. Y. B. Sc. Microbiology

References:
Experiment 1. Staining Techniques
1. Robert Cruickshank, Duguid J.P., Marmilon B.P. and Swain R.H.A. (1975). Medical
Microbiology, The Practice of Medical Microbiology. Churchill Livingstone, Edinburgh London
And New York.

Experiment 2. Air sampling using an air sampler:

1. Chosewood L. C. and Wilson D. E. (2007). Biosafety in Microbiological and Biomedical
Laboratories. DIANE Publishing Company.USA
2. Crawford R. L. and Garland J. L. (2007). Manual of Environmental Microbiology. United
States: ASM Press.
3. Geis A. P. (2020).Cosmetic Microbiology: A Practical Approach. United States:CRC
Press.
4. Nakatsu C. H., Yates M. V., Miller R. V. and Pillai S. D. (2020). Manual of Environmental
Microbiology. United States:Wiley.
5. Pepper I. L., Brendecke J. W. and Gerba C. P. (2011). Environmental Microbiology: A
Laboratory Manual. Netherlands: Elsevier Science.
6. WHO Guidelines for Indoor Air Quality: Dampness and Mould. (2009). Philippines: WHO.
Experiment 3. Air Flora:
1. Aneja K. R. (2007). Experiments in Microbiology, Plant Pathology and Biotechnology.
New Age International, New Delhi, India
2. Cox C. C. and Wathes C. M. (2020). Bioaerosols Handbook. United States: CRC Press.
3. Saxena J., Baunthiyal M. and Ravi I. (2015). Laboratory Manual of Microbiology,
Biochemistry and Molecular Biology. Scientific Publishers, Jodhpur, Rajasthan, India.
4. Verma A. S., Das S., and Singh A. (2014). Laboratory Manual for Biotechnology. S Chand
and Company Limited, New Delhi, India
Experiment 4. Bacteriological tests for potability of water
1. Aneja K. R. (2007). Experiments in Microbiology, Plant Pathology and Biotechnology.
New Age International, New Delhi, India
2. Atlas R. M. (1986; Digitized 2007). Basic and Practical Microbiology. United Kingdom:
Macmillan.
3. Dubey R. C. and Maheshwari D. K. (2002). Practical Microbiology. S. Chand and
Company Limited, New Delhi, India
4. Nollet L. M. L. and De Gelder L. S. P. (2013). Handbook of Water Analysis, Third Edition.
United States:Taylor and Francis.
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CBCS: 2019 Pattern S. Y. B. Sc. Microbiology
Experiment 5. Induction of mutations:
1. Bisen P. S. (2014). Laboratory Protocols in Applied Life Sciences. United Kingdom: CRC
Press.
2. Gunasekaran P. (2007). Laboratory Manual In Microbiology. New Age International (P)
Limited New Delhi, India
Experiment 6.
6. i. a) Azotobacter species:
1. Aneja K. R. (2007). Experiments in Microbiology, Plant Pathology and Biotechnology.
New Age International, New Delhi, India

2. Dubey R. C. and Maheshwari D. K. (2002). Practical Microbiology. S. Chand and

Company Limited, New Delhi, India
3. Gunasekaran P. (2007). Laboratory Manual In Microbiology. New Age International (P)
Limited New Delhi, India
6. i. b) Rhizobium species:
1. Aneja K. R. (2007). Experiments in Microbiology, Plant Pathology and Biotechnology.
New Age International, New Delhi, India
2. Dubey R. C. and Maheshwari D. K. (2002). Practical Microbiology. S. Chand and
Company Limited, New Delhi, India
3. Gunasekaran P. (2007). Laboratory Manual In Microbiology. New Age International (P)
Limited New Delhi, India
6. ii. Blue Green Algae (Cyanobacteria):
1. Aneja K. R. (2007). Experiments in Microbiology, Plant Pathology and Biotechnology.
New Age International, New Delhi, India
2. Bisen P. S. (2014). Laboratory Protocols in Applied Life Sciences. United Kingdom:
CRC Press.
3. Dubey R. C. and Maheshwari D. K. (2002). Practical Microbiology. S. Chand and
Company Limited, New Delhi, India
4. Kumar V. (2012). Laboratory Manual of Microbiology. Scientific Publishers, Jodhpur,
Rajasthan, India

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