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BS in Microbiology
Overview
Course Outline
Course Information
Career
2nd Year
1st Year
2nd Year
3rd Year
4th Year
PSY 101
Total Credits 30
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12/31/23, 12:21 PM BS in Microbiology | North South University
Principles of microbiology and its application to lakes, streams, hazardous wastes, and biological treatment systems. Methods of
evaluating impact of waste waters and hazardous wastes on ecological systems. Concept of limnology, including limiting of
nutrients and control of nuisance growth. Microorganisms and some novel pollution problems; persistence and
biomagnifications of xenobiotic molecules; recalcitrant halocarbons, polychlorinated biphenyls (PCBS), alkyl benzyl sulfonates,
synthetic polymer. Prerequisite MIC201.
This course introduces statistical concepts and analytical methods as applied to data encountered in biotechnology and
biomedical sciences. It emphasizes the basic concepts of experimental design, quantitative analysis of data, and statistical
inferences. Topics include probability theory and distributions; population parameters and their sample estimates; descriptive
statistics for central tendency and dispersion; hypothesis testing and confidence intervals for means, variances, and
proportions; the chi-square statistic; categorical data analysis; linear correlation and regression model; analysis of variance;
and nonparametric methods. The course provides students a foundation to evaluate information critically to support research
objectives and product claims and a better understanding of statistical design of experimental trials for biological
products/devices.
Introduction to Diversity and Taxonomy, Microbial Evolution and Diversity, Microbial Diversification: Consequences for Earth’s
Biosphere, Microbial Evolution, Microbial Taxonomy: Taxonomic ranks, Nomenclature rules and Identification, Classification
systems, Taxonomic characteristics, Major methods used in Taxonomy, Divisions of Life, Classification of bacteria based on
Bergey’s manual (Determinative), Classification of bacteria based on Bergey’s manual (Systematic)
Introduction to metabolisms: Overview, types of metabolic reactions, classification of microorgansisms based on carbon and
energy sources; Bioenergetics: Biological Energy Transformation- laws of Thermodynamics, Entropy, Enthalpy, Gibbs free energy
and related mathematics; Carbohydrate metabolism: Glycolsis- Embden-Meyerhof-Parans (EMP) Pathway, Entner-Doudoroff
(ED) pathway, Tricarboxylic Acid (TCA) Cycle, Gluconeogenesis, Metabolism of fructose, gluconate, lactose. Hexose Mono-
phosphate (HMP) pathway, Glyoxalate cycle; Membrane transport system; Amino acid biosynthesis: Biosynthetic pathways of
alanine, valine, leucine, aspartate, asparagine, lysine, methionine, isoleucine, glutamate, glutamine, arginine, proline, serine,
glycine, cysteine; Nucleic acid biosynthesis: De novo and Salvage pathways of nucleotide biosynthesis and their regulation;
Anaerobic metabolic processes: Carbohydrate fermentation by bacteria and yeast under anaerobic condition; Lipid
biosynthesis: Structure and biological function of lipids. Fatty acid and phospholipid biosynthesis; Biological nitrogen fixation;
Carbon di-oxide fixation by plants (Photosynthesis).
Important historical developments in genetics, particularly the contributions of Mendel, and Watson & Crick; and important
research advances; Analysis of genetic problems using Punnett squares to illustrate Mendelian principles; Chromosome basis of
inheritance, sex determination and sex linkage; types of chromosomal aberrations and their consequences; molecular genetics,
gene function, the structure of genetic material; the organization of DNA in chromosomes; structure and replication of DNA,
concepts of protein synthesis, gene expression, mutations, and genetic engineering; transcription and the structural and
functional features of the transcripts; genetic code and the translation of the genetic message.
General Microbiology I: Media for the Routine cultivation of Bacteria , Preparation of Differential and Selective Media, Enumeration
of the total number of bacterial cells by direct and indirect methods. Culture methods-pour plate, spread plate and streak plate
methods; Special culture methods-anaerobic and enrichment culture techniques. Staining techniques- positive staining,
negative staining, differential staining, endospore staining, flagella staining and capsule staining. Preservation and
maintenance of bacterial culture; Microbial Physiology: Effect of temperature, moist heat, osmotic pressure and pH on the
growth of vegetative cells and spore of bacteria; Metabolic Activities of Microorganisms: Hydrolysis of Large Biopolymers
Fermentation of Carbohydrates Respiration of Carbohydrates Utilization of Amino Acids Utilization of Citrate, Gelatin and Urea,
Presumptive Identification of Unknown Bacteria Based on their Biochemical Activities.
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12/31/23, 12:21 PM BS in Microbiology | North South University
Thermodynamics - First law, Second law and Introduction, definitions, Thermodynamic terms and basic concepts;
Thermochemistry, Exothermic and endothermic reactions, standard enthalpy of formation, thermochemical equations; The
nature of chemical equilibrium, law of mass action, equilibrium constant, relationship between ∆G and Keq, effect of
temperature and pressure, Le Chatelier’s principle,, Chemical Kinetics and it Definition, reaction rate, rate laws, order reactions,
molecularity of a reaction, pseudo first order reaction, half-life, Catalysis, Photochemistry and Spetrophotometry, Transmittance
and absorbance, Beer-Lambert law, Properties of liquids, Acids and bases.
This course is a comprehensive introduction in fundamental aspects of biological chemistry, for freshmen students. Knowledge
of structure, functionality and reactivity of the organic molecules is vital for understanding the mechanism of numerous
biological processes and biochemical reactions. This course provides a foundation of organic chemistry, i.e. the understanding
of structure, properties, interactions, transformations and nomenclature of organic compounds. The Students attending the
course will be able to recognize the major functional groups, write systematic names, draw accurate structures, correlate
molecular structure with physical and chemical properties, categorize reactions by type, write or recognize essential reaction
mechanisms, and recognize the structures and functions of the main classes of biomolecules.
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