Cell Diffusion & Permeability - Basic Version
Cell Diffusion & Permeability - Basic Version
Cell Diffusion & Permeability - Basic Version
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This cell membrane is semi-permeable, meaning that some molecules easily move across the cell membrane, some cannot. When solutions containing different amounts of ingredients are separated by a semipermeable membrane, diffusion of molecules occurs. Diffusion is the movement of molecules from an area of high concentration to an area of low concentration. For example, diffusion can occur across biological membranes or down open spaces in the cell.
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http://www.occc.edu/biologylabs/Images/Cells_Membranes/diffusion.gif
There are 3 main types of diffusion: o Passive Transport is when molecules diffuse without the use of energy from the cell, from areas of high amounts to areas of low amounts. o Active Transport is when molecules diffuse with the use of energy from the cell (and a special pump), from areas of low amounts to areas of high amounts. o Facilitated Transport is when molecules diffuse from areas of high amounts to areas of low amounts without the use of energy from the cell, but with the help of proteins that form channels across the cell membrane. Remember that to facilitate means to help so sometimes molecules need help getting across the cell membrane and special proteins facilitate this!
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Materials:
1. 2. 3. 4. 5. Graduated Cylinder (or 25mL beaker) Plastic Sandwich Bag Twist Tie 500mL Beaker or Flask Iodine Solution Liquid Starch Gloves (one pair per student)
Procedure:
Pour approximately 50mL of water into a plastic sandwich bag and add 10mL of starch Secure bag with the twist tie and shake gently to mix the starch. Put on gloves. Pour 250mL of water into a 500mL beaker. Add 15 drops of iodine. Place the sandwich bag of starch solution into the beaker of iodine solution.
6. 7. 8. 9.
Allow the sandwich bag to soak in the iodine solution for at least 20 minutes. Record any observations noted at the beginning, during, and at the end of the experiment. In between observations, please continue to Part 2: Cell diffusion. Once you are finished with recording your observations for Part 1: Semi-Permeability, you may answer the concept questions below the data table. Initial Cell Semi-Permeability Observations During End
Concept Questions:
1. What cellular structure does the sandwich bag model?
2. What observations did you make that occurred outside of the sandwich bag? What observations did you make that occurred inside the sandwich bag?
3. What property of the sandwich bag allows for the results you observed during the experiment and recorded for Concept Questions #2 above?
In the Cell:
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http://paramedicine101.com/files/2010/10/Osmotic_pressure.png
The egg:
Materials:
1. 3 chicken eggs with shells dissolved by vinegar 2. White vinegar 3. 3 clear plastic cups 4. Light Corn Syrup (enough to completely cover eggs) 5. Liquid egg whites (enough to completely cover eggs) 6. Distilled Water (enough to completely cover eggs) 7. String 8. Rulers 9. Triple-beam balance OR Rudimentary Scale (If scale is unavailable, only measure circumference).
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Problem:
What will happen to eggs (cells) that are separately placed in syrup, distilled water, and liquid egg whites?
Hypotheses:
1.) If I place an egg in syrup, then _____________________________________________________________________________, because ______________________________________________________________________. 2.) If I place an egg in distilled water, then _____________________________________________________________________________, because ______________________________________________________________________. 3.) If I place an egg in liquid egg whites, then _____________________________________________________________________________, because ______________________________________________________________________.
PERCENT CHANGE = Final Circumference Initial Circumference ---------------------------------------------------------X 100 Initial Circumference And PERCENT CHANGE = Final Mass Initial Mass ---------------------------------- X 100 Initial Mass
Data Table: Egg Measurements Pre/Post Diffusion Initial Final Measurement Measurement Egg #1: Corn Syrup Egg #2: Distilled Water Egg #3: Liquid Egg Whites Circumference (cm) Mass (g) Circumference (cm) Mass (g) Circumference (cm) Mass (g)
Change (cm/g)
Concept Questions:
1. What role does vinegar play in the investigation? In other words, what did the vinegar do to the eggshells?
environment. This will cause the cell to gain/lose (circle 1 answer) water because the cell is hypertonic/hypotonic/isotonic (circle 1 answer) when compared to its environment. 3. A cell placed in distilled water is in a(n) hypertonic/hypotonic/isotonic (circle 1 answer) environment. This will cause the cell to gain/lose (circle 1 answer) water because the cell is hypertonic/hypotonic/isotonic (circle 1 answer) when compared to its environment. 4. A cell placed in egg whites is in a(n) hypertonic/hypotonic/isotonic (circle 1 answer) environment. This will cause the cell to maintain equilibrium because the flow of water is the same/different (circle 1 answer) on both sides of the biological membrane.
5. Draw illustrations depicting the final outcomes of each egg after soaking in their respective solutions. Egg in corn syrup Egg in distilled water Egg in liquid egg whites