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    Iodine Test For Starch

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    Noora Mubarak
    The iodine test for starch is used to determine the presence of starch in biological materials like foods and plant leaves. Starch is a polysaccharide made of glucose units joined by glycosidic bonds that form either linear or highly branched molecules called amylose and amylopectin. The test involves adding iodine solution to a solid or liquid sample, which will turn blue-black if starch is present, indicating a positive result. Only samples from plants or plant-based foods should test positive for starch.

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    Iodine Test For Starch

    Uploaded by

    Noora Mubarak
    577 views2 pages
    The iodine test for starch is used to determine the presence of starch in biological materials like foods and plant leaves. Starch is a polysaccharide made of glucose units joined by glycosidic bonds that form either linear or highly branched molecules called amylose and amylopectin. The test involves adding iodine solution to a solid or liquid sample, which will turn blue-black if starch is present, indicating a positive result. Only samples from plants or plant-based foods should test positive for starch.

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    Iodine Test for Starch

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    The iodine test for starch is used to determine the presence of starch in biological materials like foods and plant leaves. Starch is a polysaccharide made of glucose units joined by glycosidic bonds that form either linear or highly branched molecules called amylose and amylopectin. The test involves adding iodine solution to a solid or liquid sample, which will turn blue-black if starch is present, indicating a positive result. Only samples from plants or plant-based foods should test positive for starch.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    577 views2 pages

    Iodine Test For Starch

    Uploaded by

    Noora Mubarak
    The iodine test for starch is used to determine the presence of starch in biological materials like foods and plant leaves. Starch is a polysaccharide made of glucose units joined by glycosidic bonds that form either linear or highly branched molecules called amylose and amylopectin. The test involves adding iodine solution to a solid or liquid sample, which will turn blue-black if starch is present, indicating a positive result. Only samples from plants or plant-based foods should test positive for starch.

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    © All Rights Reserved
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    Iodine Test for Starch

    The Iodine Test for Starch is used to determine the presence of starch in biological
    materials. At this level, you will be testing for the presence of this complex carbohydrate in
    foods, or in leaves as part of a photosynthesis experiment.
    If you study chemistry as well, you should pay attention principle of the test, as it helps
    explain how starch acts as an indicator for Redox Titrations or in the 'Iodine Clock Reaction'.
    The sole reagent required for the test is bench iodine solution 0.1 M potassium triiodide
    solution.

    Procedure
    Solid Sample'
    1.Peel off the skin of any vegetables e.g. potato and fruits as these are often
    impermeable. Use a clean spatula to remove samples of powdered food. Avoid cross
    contamination with other foods.
    2.Add a few ( 2-3) drops of bench iodine solution potassium to a piece of solid food on
    a white tile.
    3.Make observations.
    Liquid Sample
    N.B. The iodine test for starch cannot be performed on very dark liquids which do not permit
    the obervation of a colour change.
    1.Add 10 cm3 of the liquid food sample to a clean, dry test tube.
    2.Add about 5 drops of iodine solution to the test tube.
    3.Note any colour changes.
    4.To prepare a control, perform steps 1 -3 for de-ionized water.

    Observations and Interpretation

    TEST OBSERVATION INFERENCE


    POSITIVE A blue-black colour develops Starch is present
    NEGATIVE No change/ Iodine remains brown Starch is not present

    Recall that starch is a storage molecule found only in plants. Only plants and plant-based
    foods should test positive for the presence of starch.
    Principle of the Iodine Test for Starch
    Starch is a polysaccharide, consisting of glucose units joined together by glycosidic bonds.
    The chains formed during the condensation reaction are either linear or
    highly branched molecules.
    Linear - both straight and helical - molecules of starch are referred to as Amylose

    whereas branched molecules are called Amylopectin.

    Iodine on its own (small non-polar molecule) is insoluble in water.Therefore Potassium


    triiodide solution - Iodine dissolved in potassium iodide solution - is used as a reagent in the
    test.
    To be more specific, potassium iodide dissociates, and then the Iodide ion reacts reversibly
    with the Iodine to yield the the triiodide ion. A further reaction between a triiodide ion and an
    iodine molecule yields the pentaiodide ion.
    Since molecular iodine is always present in solution, the bench iodine solution appears
    brown; the iodide and triiodide pentaiodide ions are colourless.
    Foods which are high in amylose have more intense blue-black colour. As the Beer-Lambert
    Law is obeyed spectrometric analyses can quantify the quantity of amylose in starches.

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