2545 Practical

B.Sc.
II Year (Inorganic Practical)
What is Titration?
Titration is a quantitative chemical analysis. It is used to determine an unknown

concentration of a known substance in a sample.
The basic principle of the titration is the following:
A solution - a so called titrant or standard solution - is added to sample to be
analyzed. The titrant contains a known concentration of a chemical which reacts
with the substance to be determined. The titrant is added by means of a burette.
A burette is a device which allows to exactly measure the quantity (volume) of
the titrant added. Due to the chemical reaction taking place in the sample to be
analysed, the characteristics of the sample changes. Hence it is volumetric
analysis.
Burette
This change of the characteristics can be detected either by a so called color
indicator or a sensor:
• A color indicator changes its color as soon as all the substance contained
in the sample has reacted with the titrant added.
The concentration of the substance contained in the sample can then be
calculated based on the volume of the titrant which was required to add until all
the substance had reacted.
Terms for varieties of titration can reflect the nature of the reaction between A
and B. Thus, there are acid-base, complexometric and oxidation-reduction
titrations.
Conical flask
Standard solutions are prepared using standard substances in one of several ways.
A primary standard is a substance of known high purity which may be dissolved
in a known volume of solvent to give a primary standard solution. If
stoichiometry is used to establish the strength of a titrant, it is called a secondary
standard solution. The term secondary standard can also be applied to a substance
whose active agent contents have been found by comparison against a primary
standard.
End point and Equivalent point:

The main difference between equivalence and endpoint is that the equivalence
point is a point where the chemical reaction comes to an end while
the endpoint is the point where the colour change occurs in a system.
For a reaction, a stage which shows the completion of a particular reaction is
known as end point. Equivalence point is a stage in which the amount of reagent
added is exactly and stoichiometrically equivalent to the amount of the reacting
substance in the titrated solution. The end point is detected by some physical
change produced by the solution, by itself or more usually by the addition of an
auxiliary reagent known as an 'indicator'. The end point and the equivalence
point may not be identical. End point is usually detected only after adding a
slight excess of the titrant. In many cases, the difference between these two will
fall within the experimental error.
Indicator:
It is a chemical reagent used to recognize the attainment of end point in a titration.
After the reaction between the substance and the standard solution is complete,
the indicator should give a clear colour change.
When a titration is carried out, the free energy change for the reaction is always
negative.
That is, during the initial stages of the reaction between A & B, when the titrant
A is added to B the following reaction takes place.
Equilibrium constant,
a = activity co-efficient.
Large values of the equilibrium constant K implies that the equilibrium

concentration of A & B are very small at the equivalence point. It also indicates
that the reverse reaction is negligible and the product C & D are very much more
stable than the reactants A & B. Greater the value of K the larger the magnitude
of the negative free energy change for the reaction between A & B. Since,
Where,
R = Universal gas Constant = 8.314 JK-1mol-1,

T = Absolute Temperature.
The reaction of the concentration of A & B leads to the reduction of the total free
energy change. If the concentrations of A & B are too low the magnitude of the
total free energy change becomes so small and the use of the reaction for titration
will not be feasible.
Expressions of Concentration of Solutions:
The concentration or strength of solution means the amount of solute present in a

given amount of the solution. The concentration may be expressed in physical or
chemical units.
1. Normality (N): It is defined as number of gram equivalents of the solute

present in 1 litre (1000mL.) of the solution. If W g of solute of equivalent
weight E is present in V mL of the solution, the normality of the solution
is given by:
2. Molarity (M): It is defined as the number of moles of the solute present in

1 litre (or 1000 mL) of the solution. A one molar solution contains 1 mole
of the solute dissolved in 1 litre of the solution.
3. Molality (m): It is defined as the number of moles of solute dissolved in

1000 g of the solvent. One molal solution contains one mole of the solute
dissolved in 1000 g of the solvent.
Normal solution:
A solution containing one gram equivalent weight of the solute dissolved per litre
is called a normal solution; e.g. when 40 g of NaOH are present in one litre of
NaOH solution, the solution is known as normal (N) solution of NaOH. Similarly,
a solution containing a fraction of gram equivalent weight of the solute dissolved
per litre is known as subnormal solution. For example, a solution of NaOH
containing 20 g (1/2 of g eq. wt.) of NaOH dissolved per litre is a sub-normal
solution. It is written as N/2 or 0.5 N solution.
Formulae used in solving numerical problems on volumetric analysis;
1. Strength of solution = Amount of substance in g litre-1.
2. Strength of solution = Amount of substance in g moles litre-1.
3. Strength of solution = Normality × Eq. wt. of the solute = molarity × Mol.

wt. of solute.
4. Molarity = Moles of solute/Volume in litre.
5. Number of moles = Wt.in g/Mol. wt = M × V (initial) = Volume in
litres/22.4 at NTP (only for gases).
6. Number of milli moles = Wt. in g × 1000/mol. wt. = Molarity × Volume in

mL.
7. Number of equivalents= Wt. in g/Eq. wt = x × No. of moles × Normality ×
Volume in litre (Where x = Mol. wt/Eq. wt).
8. Number of mill equivalents (meq.) = Wt. in g × 1000 / Eq. wt = normality

× volume in mL.
9. Normality = x × No. of mill moles (Where x = valency or change in oxi.

number).
10.Normality formula, N1V1 = N2V2, (Where N1, N2 → Normality of titrant

and titrate respectively, V1, V2 → Volume of titrant and titrate
respectively).
11.% by weight = Wt. of solvent/Wt. of solution × 100 .
A solution is a homogeneous mixture of two or more components, the

composition of which may be changed. The substance which is present in smaller
proportion is called the solute, while the substance present in large proportion is
called the solvent.
Acid base titration:
The chemical reaction involved in acid-base titration is known as neutralisation

reaction. It involves the combination of H3O+ ions with OH- ions to form
water. In acid-base titrations, solutions of alkali are titrated against standard acid
solutions. The estimation of an alkali solution using a standard acid solution is
called acidimetry. Similarly, the estimation of an acid solution using a standard
alkali solution is called alkalimetry.
The Theory of Acid–Base Indicators:
Ostwald, developed a theory of acid base indicators which gives an explanation

for the colour change with change in pH. According to this theory, a hydrogen
ion indicator is a weak organic acid or base. The undissociated molecule will have
one colour and the ion formed by its dissociation will have a different colour.
Let the indicator be a weak organic acid of formulae HIn. It has dissociated into
H+ and In- . The unionized molecule has one colour, say colour (1), while the ion,
In- has a different colour, say colour (2). Since HIn and In- have different colours,
the actual colour of the indicator will dependent upon the hydrogen ion
concentration [H+]. When the solution is acidic, that is the H+ ions present in
excess, the indicator will show predominantly colour (1). On other hand, when
the solution is alkaline, that is, when OH- ions present in excess, the H+ ions
furnished by the indicator will be taken out to form undissociated water.
Therefore there will be larger concentration of the ions, In-. thus the indicator will
show predominantly colour (2).
Some indicators can be used to determine pH because of their colour changes
somewhere along the change in pH range. Some common indicators and their
respective colour changes are given below.
Indicator Colour on Acidic Range of Colour Colour on Basic

Side Change Side
Methyl Violet Yellow 0.0 - 1.6 Violet
Bromophenol Blue Yellow 3.0 - 4.6 Blue
Methyl Orange Red 3.1 - 4.4 Yellow
Methyl Red Red 4.4 - 6.2 Yellow
Litmus Red 5.0 - 8.0 Blue
Bromothymol Blue Yellow 6.0 - 7.6 Blue
Phenolphthalein Colourless 8.3 - 10.0 Pink
Alizarin Yellow Yellow 10.1 - 12.0 Red
i.e., at pH value below 5, litmus is red; above 8 it is blue. Between these values, it is a mixture
of two colours.
Indicators Used for Various Titrations:

1. Strong Acid against a Strong Base:
Let us consider the titration of HCl and NaOH. The pH values of different stages
of titration shows that, at first the pH changes very slowly and rise to only about
4. Further addition of such a small amount as 0.01 mL of the alkali raises the pH
value by about 3 units to pH 7. Now the acid is completely neutralized. Further
of about 0.01 mL of 0.1 M NaOH will amount to adding hydrogen ions and the
pH value will jump to about 9. Thus, near the end point, there is a rapid increase
of pH from about 4 to 9.
An indicator is suitable only if it undergoes a change of colour at the pH near the
end point. Thus the indicators like methyl orange, methyl red and
phenolphthalein can show the colour change in the pH range of 4 to 10. Thus, in
strong acid- strong base titrations, any one of the above indicators can be used.
2. Weak Acid against Strong Base:

Let us consider the titration of acetic acid against NaOH. The titration shows the
end point lies between pH 8 and 10. This is due to the hydrolysis of sodium
acetate formed. Hence phenolphthalein is a suitable indicator as its pH range is
8-9.8. However, methyl orange is not suitable as its pH range is 3.1 to 4.5.
3. Strong Acid against Weak Base:
Let us consider the titration ammonium hydroxide against HCl. Due to the
hydrolysis of the salt, NH4Cl, formed during the reaction, the pH lies in the acid
range. Thus, the pH at end point lies in the range of 6 to 4. Thus methyl orange is
a suitable indicator while phenolphthalein is not suitable.
StrongAcids StrongBases WeakAcids WeakBases

HCl NaOH Acetic acid Ammonia
HNO3 KOH Hydrocyanic acid Magnesium hydroxide
HBr etc HF Pyridine
H2SO4 Oxalic acid Sodium carbonate
HI Ethanoic acid Potassium carbonate
HClO4 etc etc
For acid base titration (procedure), Please go through the

following link:
https://www.youtube.com/watch?v=XD5GZYjtGek
Object: To determine the strength of oxalic acid solution (gm/lit)

by titrating it against approx. N/10 NaOH solution.

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B.Sc.

II Year (Inorganic Practical)

Titration is a quantitative chemical analysis. It is used to determine an unknown

End point and Equivalent point:

Large values of the equilibrium constant K implies that the equilibrium

R = Universal gas Constant = 8.314 JK-1mol-1,

Expressions of Concentration of Solutions:

The concentration or strength of solution means the amount of solute present in a

1. Normality (N): It is defined as number of gram equivalents of the solute

2. Molarity (M): It is defined as the number of moles of the solute present in

3. Molality (m): It is defined as the number of moles of solute dissolved in

Formulae used in solving numerical problems on volumetric analysis;

1. Strength of solution = Amount of substance in g litre-1.

2. Strength of solution = Amount of substance in g moles litre-1.

3. Strength of solution = Normality × Eq. wt. of the solute = molarity × Mol.

6. Number of milli moles = Wt. in g × 1000/mol. wt. = Molarity × Volume in

8. Number of mill equivalents (meq.) = Wt. in g × 1000 / Eq. wt = normality

9. Normality = x × No. of mill moles (Where x = valency or change in oxi.

10.Normality formula, N1V1 = N2V2, (Where N1, N2 → Normality of titrant

11.% by weight = Wt. of solvent/Wt. of solution × 100 .

A solution is a homogeneous mixture of two or more components, the

The chemical reaction involved in acid-base titration is known as neutralisation

The Theory of Acid–Base Indicators:

Ostwald, developed a theory of acid base indicators which gives an explanation

Indicator Colour on Acidic Range of Colour Colour on Basic

Indicators Used for Various Titrations:

2. Weak Acid against Strong Base:

3. Strong Acid against Weak Base:

StrongAcids StrongBases WeakAcids WeakBases

For acid base titration (procedure), Please go through the

Object: To determine the strength of oxalic acid solution (gm/lit)

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