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    Lab2 Cheek Cell A

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    Blue Sky
    This document describes a laboratory activity where students observe human cheek cells under a microscope. The objectives are to observe and identify major animal cell structures, prepare a slide of human cheek cells, and illustrate eukaryotic and prokaryotic cells. The procedure involves staining cheek cells scraped from the inside of one's cheek with methylene blue and observing them under different magnifications of a light microscope. Students are asked to sketch their observations, describe what they see, and answer questions about cell structure, staining, visible organelles, and the function of cells in the mouth.

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    Lab2 Cheek Cell A

    Uploaded by

    Blue Sky
    36 views3 pages
    This document describes a laboratory activity where students observe human cheek cells under a microscope. The objectives are to observe and identify major animal cell structures, prepare a slide of human cheek cells, and illustrate eukaryotic and prokaryotic cells. The procedure involves staining cheek cells scraped from the inside of one's cheek with methylene blue and observing them under different magnifications of a light microscope. Students are asked to sketch their observations, describe what they see, and answer questions about cell structure, staining, visible organelles, and the function of cells in the mouth.

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    LAB2-CHEEK-CELL-A

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    This document describes a laboratory activity where students observe human cheek cells under a microscope. The objectives are to observe and identify major animal cell structures, prepare a slide of human cheek cells, and illustrate eukaryotic and prokaryotic cells. The procedure involves staining cheek cells scraped from the inside of one's cheek with methylene blue and observing them under different magnifications of a light microscope. Students are asked to sketch their observations, describe what they see, and answer questions about cell structure, staining, visible organelles, and the function of cells in the mouth.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    36 views3 pages

    Lab2 Cheek Cell A

    Uploaded by

    Blue Sky
    This document describes a laboratory activity where students observe human cheek cells under a microscope. The objectives are to observe and identify major animal cell structures, prepare a slide of human cheek cells, and illustrate eukaryotic and prokaryotic cells. The procedure involves staining cheek cells scraped from the inside of one's cheek with methylene blue and observing them under different magnifications of a light microscope. Students are asked to sketch their observations, describe what they see, and answer questions about cell structure, staining, visible organelles, and the function of cells in the mouth.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 3

    Name: _______________________ Date: ___________

    Instructor: Mrs. Cathelyn C. Mariano

    Laboratory Activity 2
    Human Cell

    INTRODUCTION

    There are three domains of living organisms: eukarya, bacteria, and archaea. These domains are
    actually based on differences in the structures of cells! All organisms in the Domain Eukarya are
    made up of cells with a nucleus that contains genetic information. Some eukaryotes are tiny
    organisms that are made up of only one cell, but most are made up of trillions of cells. Examples of
    eukarya include fungi, plants, animals, protozoans. Bacteria and archaea are Prokaryotes, these are
    tiny organisms that are made up of only one cell with no nucleus. Instead of being contained in a
    nucleus, their genetic material just floats around inside the cell. Bacteria and archaea are in different
    domains because they have different kinds of molecules inside their cells.

    Objectives:
    1. Observe and identify major animal cell structures and to relate the structure of the cell to
    its function.
    2. Prepare a fresh-mount slide of animal cell using human cheek cells.
    3. Illustrate/draw eukaryotic and prokaryotic cells.

    Materials: toothpick methylene blue


    slide & cover slip human cheek cells

    Procedure:
    1. Put a drop of methylene blue on a slide. Caution: methylene blue will stain clothes and skin.
    2. Gently scrape the inside of your cheek with the flat side of a toothpick. Scrape lightly.
    3. Stir the end of the toothpick in the stain to spread the cheek cells.
    4. Place a coverslip onto the slide.
    5. Use the SCANNING objective first and use the coarse adjustment knob to bring the slide into
    focus. Cells will appear very tiny at this magnification.
    6. Switch to LOW POWER and use the coarse adjustment knob. Cells should be visible, but they
    will be small and look like nearly clear purplish blobs. If you are looking at something very dark
    purple, it is probably not a cell.
    7. Once you think you have located a cell, switch to HIGH POWER and focus using the fine
    adjustment knob. (Remember, do NOT use the coarse adjustment knob at this point.)
    Observations:
    Sketch the cell at low and high power. Label the nucleus, cytoplasm, and cell membrane of
    a single cell. Write a brief description of your observations & magnification next to each
    drawing.

    1. 4x magnification
    Total magnification:___________
    Description:
    __________________________________________________

    2. 10x magnification
    Total magnification:___________
    Description:
    __________________________________________________

    3. 40x magnification
    Total magnification:___________
    Description: __________________________________________________

    Guide Questions:

    1.Describe the shape(s) of the cell membrane. Were all the cells the same shape? Explain why.

    2.Explain the reason(s) why you used iodine stain. What would it have looked like without the
    stain?

    3. Why is methylene blue necessary?

    4. The light microscope used in the lab is not powerful enough to view other organelles in the
    cheek cell. What parts of the cell were visible.
    5. List 2 organelles that were NOT visible but could be found in cells if you had a microscope with
    a better magnification.

    6. Keeping in mind that the mouth is the first site of chemical digestion in a human. Your saliva
    starts the process of breaking down the food you eat. Keeping this in mind, what organelle do you
    think would be numerous inside the cells of your mouth?

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