LEARNING ACTIVITY SHEET

SCIENCE 7
Quarter 2 Week 2
Preparing a Wet Mount, Focusing Specimen and Magnification

L I. LEARNING SKILLS
Focus specimens using the compound microscope (S7LT-IIb-2)

At the end of the learning activity sheet, you should be able to:
1. Prepare a wet mount;
2. Make a DIY (Do It Yourself) microscope;
3. Describe the image produced under different objectives;
4. Compare the images produced; and
5. Solve for the total magnification of the specimen under different
objectives.

II. INTRODUCTORY CONCEPT

The world is full of wonderful things. But many of these are not seen with our
naked eyes. Isn’t it an exciting prospect to see how a single strand of your hair would
look if magnified, or see what microorganisms look like? Ever wondered what
microbiologists see every time they are using the microscope? What corona virus
looks like? These are all possible with the use of a microscope if you know how to
manipulate it and prepare microscope slides, specifically wet mounts properly.
How do we prepare wet mounts? Do you know which parts of the microscope
are responsible for magnifying the specimen? How do we focus the specimen under
the microscope? What is magnification? What are the things that we should follow to
do all of these properly?
Perform the activities and let us see those wonderful things up close!

III. ACTIVITIES

PRACTICE TASK

Activity1A: ColorDEFINEd!

Directions: Read the word inside the first box and color its definition by
choosing on the second set of boxes and the sample image on the last set of boxes.
Do the task on the worksheet provided.

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LEARNER’S COPY
NOTE: Inverted – Red Enlarged – Blue Upright – Yellow Reversed – Green

Please refer to the image presented below as a guide.

a Original object
Set A Set B Set C

Inverted
The image is larger a
than the object.

The image is formed

Enlarged switched from left to
a
right.

The image is upside

Upright down.

The image is right side

Reversed up.

Activity 1B: LET’S DO THE MATH!

Direction: Given the formula, solve the following word problems. Write your
solution inside the box provided under each item on the worksheet provided.

FORMULA:
𝑚𝑎𝑔𝑛𝑖𝑓𝑖𝑐𝑎𝑡𝑖𝑜𝑛 𝑜𝑓 𝑡ℎ𝑒 𝑚𝑎𝑔𝑛𝑖𝑓𝑖𝑐𝑎𝑡𝑖𝑜𝑛 𝑜𝑓 𝑡ℎ𝑒
𝑡𝑜𝑡𝑎𝑙 𝑚𝑎𝑔𝑛𝑖𝑓𝑖𝑐𝑎𝑡𝑖𝑜𝑛 𝑜𝑓 𝑡ℎ𝑒 𝑠𝑝𝑒𝑐𝑖𝑚𝑒𝑛 = ×
𝑒𝑦𝑒𝑝𝑖𝑒𝑐𝑒 𝑜𝑏𝑗𝑒𝑐𝑡𝑖𝑣𝑒
Note: Microscope has typically three objectives. These are the scanner, LPO and
HPO.

PROBLEM NO. 1:
A student examined an object under the microscope the eyepiece is 5x while
the LPO is 10x. How many times was the object magnified?

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PROBLEM NO. 2:
You were given a slide which contained a microorganism. You were asked to
examine the microorganism under the HPO. Your eyepiece is 10x and the HPO is
45x. How many times was the microorganism magnified?

PROBLEM NO. 3:
A student studied a prepared slide of an animal cell. The eyepiece is 5x and
the HPO is 60x. How many times was the object magnified?

Note: The following are hands-on activities to understand the concept of

Focusing Specimens under the Microscope. Kindly prepare the
materials needed for each activity. Note: Seek help from a
parent/guardian when handling the materials especially sharp objects.

Activity 2A: DIY: Microscope in the House!

Directions: Prepare the materials needed and follow the instructions stated.
Materials Needed:
✓Clear plastic drinks bottle ✓Scissors / Cutter ✓Water
✓ Small Box (example: Shoe Box) ✓ Tape ✓Dropper

Procedure:
1. Cut a narrow strip of the thin plastic
bottle, about 10 by 3 centimeters.

2. Next, tape one end of strip firmly to the

side of the box, with the other end
hanging over.

3. Put at least three drops of water to the

hanging end of the plastic strip.

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 4. To use the water lens microscope, put
your eyes close to the water lens.
5. Hold an object under it. Move the object
up and down until you can see the
object clearly.

Photos taken by Clarie Vie C. Sapalaran

Activity 2B: DIY: Wet Mount Preparation

Directions: Prepare the needed materials stated below and carefully follow
the instructions.

Materials Needed:
✓ Clear empty plastic bottle ✓ Water ✓Newspaper
✓ Scissors / Cutter ✓Dropper ✓Tissue/Clean Cloth
✓Tweezers ✓Pencil

Procedure:
1. Cut a strip of plastic from the bottle
preferably 3” by 1” for the glass slide and
another strip with 1” by 1” measurement
for the coverslip. Look for the part which
is clear and flat.

2. Using a tissue or clean cloth wipe the

strip to make it free from finger stains.

3. Cut a small letter “a” in the newspaper

and place it in the center of the slide
using a tweezer.

4. Using a dropper, add a drop of clean

water over the specimen.

5. Slowly lower the other edge of the cover

slip until it rests on the water and on the
piece of newspaper cut out.

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 6. Make the bubble move towards the edge
of the cover slip by tapping it with the
eraser-end of a pencil
7. Now, observe the specimen under your
DIY microscope then answer the activity
that follows. Write your answer on the
worksheet provided
Photos taken by Clarie Vie C. Sapalaran

Specimen observed: __________________________

Illustrate:

Image seen by your naked eye Image seen under DIY Microscope
Guide Questions:
1. What differences did you observe from the object as seen by your naked eye
and under the DIY microscope?

2. Have you encountered any problem while doing the activity? If yes, state the
problem and your solution.

Activity 3: “Focus on Me!”

Directions: Observe the specimen and write your answers on the worksheet
provided.

Images

Seen by Seen under Seen under Seen under

Eyepiece naked eye Scanner LPO HPO
(5x) (5x) (10x) (40x)

Observation
(Describe the
image)

Photos taken by Clarie Vie C. Sapalaran

Guide Questions:
1. Compare the image of the letter that you see using your unaided eye with what
you see through the microscope.

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 2. How does the view of specimen change as you increase the power of
magnification?
3. In which objective can you see the whole letter “e”?
4. What are the advantages and disadvantages of viewing specimen under HPO?
5. Compute for the total magnification of the specimen under LPO and HPO.

In a wet mount, the specimen is suspended in a drop of liquid which is usually

water and is located between the slide and cover glass. The water improves the
quality of the image and supports the specimen. Wet mounts cannot be stored over
long period of time because the water evaporates. For this reason, water is often
called the temporary mount to differentiate it from the permanent mounts, which can
be stored over long period of time.
There are different types of wet mount. Water, immersion oil and glycerin can be
used but water is the most used.

The DO’s and the Don’ts in preparing a wet mount.

a. Avoid touching the cover glass area, slides should be held by the
edges.
b. Add only one drop of clean water over the specimen.
c. Excess water should be wiped with tissue paper.
d. Use a sharp blade or scalpel in extracting a specimen to avoid
damage in it.
e. Do not apply pressure on the cover glass. It can cause breakage.
f. Wash the soiled slides with soapy water or isopropyl alcohol.

Here are the steps in Observing Specimens under a Microscope:

1. Put the slide on the stage. Make sure that the specimen is in the center of the
hole on the stage and hold firmly with the stage clips.
2. Watching from the side, carefully lower the body tube until the end of the
objective almost touches the cover slip.
3. Look through the eyepiece. Slowly turn the coarse adjustment knob upwards
to raise the objective until the image appears. Continue until you see the
image clearly. Turn the fine adjustment knob until you see the clearest image.
4. Observe closely the image.

Magnification is the amount or degree of visual enlargement of a specimen.

For certain purposes we need to know how much the specimen has been magnified.
One way to express this is through total magnification of the specimen.
The formula in computing for the total magnification:
𝑚𝑎𝑔𝑛𝑖𝑓𝑖𝑐𝑎𝑡𝑖𝑜𝑛 𝑜𝑓 𝑡ℎ𝑒 𝑚𝑎𝑔𝑛𝑖𝑓𝑖𝑐𝑎𝑡𝑖𝑜𝑛 𝑜𝑓 𝑡ℎ𝑒
𝑡𝑜𝑡𝑎𝑙 𝑚𝑎𝑔𝑛𝑖𝑓𝑖𝑐𝑎𝑡𝑖𝑜𝑛 𝑜𝑓 𝑡ℎ𝑒 𝑠𝑝𝑒𝑐𝑖𝑚𝑒𝑛 = ×
𝑒𝑦𝑒𝑝𝑖𝑒𝑐𝑒 𝑜𝑏𝑗𝑒𝑐𝑡𝑖𝑣𝑒

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 Wherein, Magnification of the Eyepiece is the number indicated on the
eyepiece while the Magnification of the Objective is the number indicated on each
objective.
Learn how to compute for the Magnification of the Specimen taken from
Techan’s Triangle in mathematics, a technique which can be used in getting the total
magnification of the specimen, magnification of eyepiece and magnification of
objective.
Procedure:

1. Carefully read and study first the problem in order to determine the required
component.
2. Cover the required component that you will look for.
Follow the two (2) Conditions. . .
a. If the remaining components are side by side, multiply it.
b. If one component is on top of the other, divide.

Total
Magnification of
the Specimen

Magnification Magnification
of the of the
Eyepiece Objective

3. Formulate the equation/formula to be used in solving the problem.

Note: In using Techan’s triangle you must consider the following:

1. In order to get the total magnification of the specimen, the magnification

of eyepiece should be multiplied to magnification of objective.
EXAMPLE. Given:
Magnification of Eyepiece = 4x
Magnification of Objective = 10x
Solution:
magnification of the magnification of the
total magnification of the specimen = ×
eyepiece objective

= (4x) x (10x)
= 40x or 40 times
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 2. To determine the magnification of eyepiece, the magnification of
objective is divided to the total magnification of the specimen.
EXAMPLE. Given:
Total Magnification of the Specimen = 40x
Magnification of Objective = 10x

Solution:
magnification of the
𝑚agnification of eyepiece = total magnification of specimen ×
objective

= (40x) / (10x)
= 4x or 4 times

3. To determine the magnification of the objective, the magnification of the

eyepiece is divided to the total magnification of the specimen.

EXAMPLE. Given:
Total Magnification of the Specimen = 40x
Magnification of Eyepiece = 4x

Solution:
total magnification of the magnification of
𝑚agnification of objective = ×
specimen eyepiece

= (40x) / (4x)
= 10x or 10 times

ASSESSMENT
1. Which of the following refers to the type of mount that uses liquid during its
preparation?
a. Dry mount c. Wet mount
b. Prepared mount d. None of the above
2. Which part of the microscope is responsible in enlarging the specimen?
a. Illuminating parts c. Mechanical parts
b. Magnifying parts d. Supporting parts
3. Which of the following statement best describes magnification?
a. Separating image details.
b. Enlarging image of a specimen.
c. The ability of a lens to enlarge specimen.
d. Displaying details of enlarge specimen.

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4. What is the position of the image produced by the microscope with respect to
the original position of the specimen?
a. Inverted b. Landscape c. Portrait d. Upright
5. What is the formula in computing the total magnification of the image in a
compound microscope?
a. total magnification of the specimen =
(magnification of the eyepiece) x (magnification of the objective)
b. total magnification of the specimen =
(magnification of the eyepiece) / (magnification of the objective)
c. total magnification of the objective =
(magnification of the eyepiece) x (magnification of Specimen)
d. total magnification of objective =
(magnification of the eyepiece) / (magnification of the specimen)
6. How do you place the cover slip into the slide?
a. drop the coverslip on top of the slide to cover the specimen
b. tilt the slide at 45° and place the coverslip on top of the specimen
c. place the coverslip on top of the specimen by adding water above it
d. slowly lower the edge of the coverslip until it all covers the specimen
7. Which of the following are the characteristics of an image formed through the
microscope?
a. Upright, enlarged c. Upright, inverted and enlarged
b. Reversed, enlarged d. Inverted, reversed and enlarged
8. Why do we need to store the microscope away from chemicals?
a. The chemicals clog the lenses.
b. The smell may stick to the microscope.
c. The fumes may corrode the microscope.
d. None of the above.
9. The following are characteristics of an image produced by a microscope
under LPO and HPO except _______.
a. Enlarged b. Inverted c. Reversed d. Upright
10. What is the magnification of the objective if you are magnifying the specimen
300x while using a 5x eyepiece?
a. 30x b. 40x c. 60x d. 80x

IV. RUBRIC FOR SCORING

V. REFERENCES

• Abramowitz, Mortimer and Davidson, Michael W. Anatomy of the Microscope:

Introduction. Accessed June 20, 2020.
https://www.olympus_lifescience.com/en/microscope-
resources/primer/anatomy/introduction

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 • The Physics Classroom. Image Formation Revisited. Accessed July 1, 2020.
https://www.physicsclassroom.com/class/refrn/Lesson-5/Image-Formation-Revisited
• Microbehunter Microscopy. Making a Wet Mount Microscope Slide. Accessed June
21, 2020 www.microbehunter/making-a-wet-mount-microscope-slide/
• New Biology Science II. Dynamic World of Life. Marren Publishing House, Inc. 9-11
• NISMED.Science and Technology II, Biology Textbook.2012 pages 13-15
• Paula Borton. Science Encyclopedia.Parragon.1999 page 233
• Asuncion, Alvie J. et al. Science 7 Learner’s Material. 2017. Pages 113-115
• Talaro. Foundations in Microbiology 6th Edition. McGraw Hill. 2008 page 73
• Abramowitz, Mortimer and Davidson, Michael W. The Concept of Magnification.
Accessed July 3, 2020. https://www.olympus-lifescience.com/en/microscope-
resource/primer/anatomy/magnification/s

Supplemental Learning Resource:

Michael Leonard Lubiano. Science 7 Quarter 2 Episode 2: Focusing specimen using a

compound microscope. Retrieved last August 23, 2021. https://youtu.be/dC0I3bmeBIY

SirBas TV. Using the Compound Microscope Science 7 Quarter 2 Module 1B. Retrieved
last August 23, 2021. https://youtu.be/oQN30kduZWY

Let me know how much you have learned from this lesson by completing the
sentence and writing your feedbacks below.

I have learned that… I wish to ask my teacher about…

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 LEARNING ACTIVITY SHEET
SCIENCE 7
Quarter 2 Week 2
WORKSHEET NUMBER 2

Name:_______________________________Grade&Section:_________________
Date:________________________________________Score:_________________

Activity1A: ColorDEFINEd!

a
The image is larger than the
Inverted object.

The image is formed switched

a
Enlarged from left to right.

The image is upside down.

Upright
The image is right side up.
Reversed
Activity 1B: LET’S DO THE MATH!
PROBLEM 1 SOLUTION:

PROBLEM 2 SOLUTION:

PROBLEM 3 SOLUTION:

Activity 2: DIY Specimen observed: __________________________

Illustrate:

1 Please answer and submit this to your teacher/ adviser.

Guide Questions:
1.

2.

Activity 3: “Focus on Me!”

Eyepiece Seen by Seen under Seen under Seen under

(5x) naked eye Scanner (5x) LPO (10x) HPO(40x)

Observation
(Describe the
image)

Guide Questions:
1.

2.

3.

4.

5.
Solution:

Assessment
1. 6.
2. 7.
3. 8.
4. 9.
5. 10.

REFLECTION

I have learned that… I wish to ask my teacher about…

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