Scribd Logo
Upload

Welcome to Scribd!

  • 17 views

    Tests For Carbohydrates, Fats and Proteins 2019-20

    Uploaded by

    vanshumsh

    Copyright:

    © All Rights Reserved
    Download as XLSX, PDF, TXT or read online from Scribd

    Tests For Carbohydrates, Fats and Proteins 2019-20

    Uploaded by

    vanshumsh
    17 views4 pages

    Original Title

    Tests for carbohydrates,fats and proteins 2019-20 (1)

    Copyright

    © © All Rights Reserved

    Available Formats

    XLSX, PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    Copyright:

    © All Rights Reserved
    Download as XLSX, PDF, TXT or read online from Scribd
    Download as xlsx, pdf, or txt
    17 views4 pages

    Tests For Carbohydrates, Fats and Proteins 2019-20

    Uploaded by

    vanshumsh

    Copyright:

    © All Rights Reserved
    Download as XLSX, PDF, TXT or read online from Scribd
    Download as xlsx, pdf, or txt
    of 4

    Tests for carbohydrates, fats and proteins

    Aim : To test the presence of carbohydrates, fats and proteins in given food stuffs.

    I. Tests for carbohydrates

    Molisch test
    To the sample add 2-3 drops of
    naphthol solution and then add 2-3
    drops of conc. H2SO4 along the sides of Purple ring is formed at the
    1 the test tube interface of two layers

    Fehling solution test


    To the given sample add
    1 mL of Fehling solution A and 1 mL of
    Fehling solution B.Heat the contents for
    2 about 2 minutes on a water bath. A brick red ppt is formed

    Benedict’s test
    To the sample add benedicts
    3 reagent and heat A brick red ppt is formed
    Tollen’s reagent test
    To the sample add 2 mL of A shiny silver mirror is
    Tollen's reagent and heat on a water formed on the inner side of
    4 bath. the test tube
    II. Test for starch

    To the sample add few drops of iodine


    1 solution. The indicates the Blue colour is formed
    III. Tests for fats
    Translucent spot test

    Place a few drops of the sampleon a A translucent spot is


    1 filter paper obtained

    Acrolein test
    To the sample adda
    few crystals of dry potassium hydrogen
    2 sulphate A pungent smell is obtained
    IV. Tests for proteins

    Biuret test
    To the sample add about 2 mL of
    10% sodium hydroxide solution. Then
    add a few drops of copper sulphate
    solution and warm the mixture for
    about 5 minutes.
    1 Violet colour is obtained
    Ninhydrin test Blue colur is formed
    To the sample add 3-drops of
    2 ninhydrin solution and heat.

    Xanthoproteic test
    To the sample Yellow colour is obtained
    add a few drops of concentrated nitric
    3 acid and heat for a few minutes
    d proteins
    en food stuffs.

    Presence of carbohydrates is
    confirmed

    Presence of carbohydrates
    (reducing sugar) is confirmed

    Presence of carbohydrates
    (reducing sugar) is confirmed

    Presence of carbohydrates
    (reducing sugar) is confirmed

    Presence of starch is confirmed

    Presence of fat is confirmed

    Presence of fat is confirmed

    Presence of protein is confirmed


    Presence of protein is confirmed

    Presence of protein is confirmed

    You might also like