EFFICACY OF ALOE VERA (Aloe barbadensis Mill) EXTRACTS AS ROOT

ENHANCER IN DRAGON FRUIT (Hylocereus undatus)

CUTTINGS AS INFLUENCED BY DIFFERENT
TIME OF SOAKING DURATION

JANETH G. PASCUAL

Undergraduate Thesis Manuscript Submitted to the College of Agriculture &

Fisheries, South East Asian Institute of Technology, Inc. In Partial
Fulfillment of the Requirement for the Degree of

BACHELOR OF SCIENCE IN AGRICULTURE

(HORTICULTURE)

JULY 2023

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 REPUBLIC OF THE PHILIPPINES
SOUTH EAST ASIAN INSTITUTE OF TECHNOLOGY INC.
National Highway, Crossing Rubber, Tupi, South Cotabato
COLLEGE OF AGRICULTURE AND FISHERIES
Email address: [email protected]

APPROVAL SHEET
UNDERGRADUATE THESIS MANUSCRIPT

Name: JANETH G. PASCUAL Degree: BSA

Major: HORTICULTURE Crop: DRAGON FRUIT

Title: EFFICACY OF ALOE VERA (Aloe barbadensis Mill) EXTRACTS AS

ROOT ENHANCER IN DRAGON FRUIT (Hylocereus undatus)
CUTTINGS AS INFLUENCED BY DIFFERENT TIME OF SOAKING
DURATION.

APPROVED BY THE GUIDANCE COMMITTEE

JOHN BERNARD L. GALLEGO, L.Agr FLOREVIE A. DEODOR, L.Agr

Adviser Statistician

_______________ _______________
Date Date

BONIFACIO V. PALES, MSc. FLOREVIE A. DEODOR, L.Agr.

Department Coordinator- Horticulture College Research Coordinator

_______________ _______________
Date Date
APPROVED BY:

LYDIA C. CANO, Ph.D

Dean, College of Agriculture and Fisheries
________________

Date

ii
 TRANSMITTAL

The thesis attached hereto, entitled,”EFFICACY OF ALOE VERA (Aloe

barbadensis Mill) EXTRACTS AS ROOT ENHANCER IN DRAGON FRUIT

( Hylocereus undatus) CUTTINGS AS INFLUENCED BY DIFFERENT TIME

OF SOAKING DURATION” prepared and submitted by JANETH G.

PASCUAL in partial fulfillment of the requirements for the degree of Bachelor

of Science in Agriculture (Horticulture) is hereby accepted:

JOHN BERNARD L. GALLEGO, L.Agr

Adviser

______________
Date

Accepted as partial fulfillment of the requirements for the degree of

Bachelor of Science in Agriculture (Horticulture).

FLOREVIE A. DEODOR, L.Agr

College Research Coordinator

________________
Date

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 BIOGRAPHICAL DATA

The researcher was born on March 3, 2001, in Tupi, South Cotabato.

She is the youngest among the three children of Mr. Jenito C. Pascual Sr. and

Petra G. Pascual.

She completed her kindergarten education at Palian Day Care Center,

followed by her elementary years at Palian Elementary School, where she

graduated with academic excellence. Continuing her academic journey, she

attended Tupi National High School for both her Junior and Senior High

School, graduating with high honors.

She pursued her college education at South East Asian Institute of

Technology Inc. as an Agriculture student, with a major in Horticulture.

JANETH G. PASCUAL
Researcher

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 ACKNOWLEDGMENT

The researcher extends heartfelt gratitude to the almighty Father for His

boundless love and unwavering guidance throughout her academic journey,

providing her with wisdom and knowledge that she deeply appreciates.

Special thanks are extended to her adviser, Sir John Bernard L.

Gallego L, Agr, for generously dedicating his time, offering valuable advice,

constructive criticism, and insightful ideas that greatly contributed to this

study's success.

Appreciation goes to Ma’am Florevie A. Deodor L, Agri, who provided

assistance in statistical analysis and offered her guidance and advice.

Heartfelt appreciation is also extended to her beloved parents, Mr.

Jenito C. Pascual Sr. and Petra G. Pascual, for their unwavering support,

assistance, and prayers. Gratitude is also extended to her cousin, Juicy G.

Ameller, for inspiring her with the idea for a possible research title.

Special recognition and thanks are extended to her fiancé, JOI

Charlbert John Z. Mayang, for his continuous support, understanding, and

encouragement from the very beginning, aiding her throughout her study.

The researcher is grateful to Mrs. Charito Z. Mayang and Mr. Albert

Mayang for generously donating aloe vera plants and dragon fruit cuttings

from their organic farm site.

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 Appreciation is also extended to her best friend, Chloe Faye, for

unwavering support and the lending of her laptop. Gratitude is also extended

to her cousins, college friends, high school friends, and classmates, whose

support, concern, and prayers were invaluable.

The researcher acknowledges the contribution of her cousins who

aided her throughout her studies. She would also like to express her gratitude

to her college friends, high school friends, and classmates for their support,

concern, and prayers.

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 TABLE OF CONTENTS

Page

PRELIMINARY PAGES

Title Page i
Approval Sheet ii
Transmittal iii
Biographical Data iv
Acknowledgement v
Table of Contents vi
List of Tables viii
List of Figures ix
List of Appendices xi
Abstract xii

CHAPTER I INTRODUCTION 1

CHAPTER II REVIEW OF RELATED LITERATURE 3

Dragon Fruit 3
Phytohormones 5
Aloe vera as root enhancer 6
CHAPTER III MATERIALS AND METHODS

Materials 9
Methods 9
Experimental Design 9
Field Lay Out of the Study 10
Sample Plot Lay-out of the Study 11
Treatments 12
Experimental Area 12
Nursery Site 13
Nursery Pattern 13
Procurement of Dragon Fruit Cutting 13
Preparation of Aloe vera Extract 14

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 Soil Media Prepapartion 14
Planting of Dragon Fruit 14
Weed Management 15
Watering 15
Treatment Application 15
Termination of the Study 16
Data Gathered 17
Number of Roots 17
Length of Roots 17
Number of Shoots 17
Number of Infected Cutting 17
Number of Non-Infected Cutting 18
Statistical Data Analysis 19

CHAPTER IV RESULTS AND DISCUSSIONS 20

Number of Roots 20
Length of Roots 22
Number of Shoots 23
Number of Infected cuttings 25
Number of Non-infected cutting 27

CHAPTER V SUMMARY, CONCLUSION AND 28

RECOMMENDATION

Summary 29
Conclusion 30
Recommendation 30

LITERATURE CITED 32

APPENDICES 35

DOCUMENTATION 45

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 LIST OF TABLES

TABLE NO. TITLE PAGE

1 The treatments used in the study. Prk 3, 12

Palian, Tupi South Cotabat. November-
December 2022.

2 Criteria for non-infected and infected cuttings 18

3 Number of roots as influence by different 20

time of soaking in Aloe vera extract

4 Length of roots as influence by different time 22

of soaking in Aloe vera extract

5 Number shoots as influence by different time 24

of soaking in Aloe vera extract

6 Number of infected cuttings as influence by 26

different time of soaking in Aloe vera extract

7 Number of non-infected cuttings as influence 27

by different time of soaking in Aloe vera
extract

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 LIST OF FIGURES

FIGURE NO. TITLE PAGE

1 Field Layout of the Study in Purok 3, 10

Brgy Palian Tupi South Cotabato
November- December 2022.

2 Plot Layout of the Study in Purok 3,Brgy 11

Palian, Tupi South Cotabato November-
December 2022.

3 Nursery Pattern in Brgy Palian Tupi 13

South Cotabato November- December
2022.

4 Nursery Establishmen. Purok 3,Brgy 46

Palian, Tupi South Cotabato November-
December 2022
5 Soil Media Preaparation in Purok 3, Brgy 46
Palian Tupi South Cotabato
6 Procurement of dragon fruit cuttings. 47
Purok 3, Brgy Palian, Tupi South
Cotabato November-December 2022
7 Preparation of aloe vera extract.Purok 3, 47
Brgy Palian, Tupi South Cotabato.
November-December 2022
8 Application of treatments in different time 48
of soaking.Purok 3, Brgy Palian Tupi
South Cotabato.November-December
2022
9 Planting of dragon fruit cuttings. Purok 3, 48
Brgy, Palian Tupi South Cotabato.
November-December 2022
10 Watering and removing of weeds. Purok 49
3, Brgy Palian Tupi South
Cotabato.November-December 2022
11 Termination of the study. Purok 3, Brgy 49
Palian, Tupi South Cotabato. November-
December 2022.

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12 Area visitation Purok 3, Brgy Palian Tupi 50
South Cotabato. December 2022.
13 Number of roots as affected by different 50
time of soaking in aloe vera extract.
Purok 3, Brgy Palian, Tupi South
Cotabato
14 Length of roots as affected by different 51
time of soaking in aloe vera extract.
Purok 3, Brgy. Palian Tupi South
Cotabato. December 2022
15 Number of shoots as affected by different 51
time of soaking in aloe vera extract.
Purok 3, Brgy Palian, Tupi South
Cotabato. December 2022
16 Number of infected cutting as affected by 52
different time of soaking in aloe vera
extract. Purok 3, Brgy Palian,Tupi South
Cotabato

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 LIST OF APPENDICES

APPENDIX NO. TITLE PAGE

1 Number of root of dragon fruit cuttings as 36

affected by different time of soaking in aloe
vera extract. Brgy Palian Tupi, South
Cotabato. December 2022

2 Number of root of dragon fruit cuttings as 37

affected by different time of soaking in aloe
vera extract. Brgy Palian Tupi, South
Cotabato. December 2022

3 Number of root of dragon fruit cuttings as 38

affected by different time of soaking in aloe
vera extract. Brgy Palian Tupi, South
Cotabato. December 2022

4 Number of root of dragon fruit cuttings as 39

affected by different time of soaking in aloe
vera extract. Brgy Palian Tupi, South
Cotabato. December 2022

5 Number of root of dragon fruit cuttings as 40

affected by different time of soaking in aloe
vera extract. Brgy Palian Tupi, South
Cotabato. December 2022

6 Application for Undergraduate Thesis 41

Manuscript Defense

7 Undergraduate Thesis Manuscript Defense 42

8 Certification of Statistician 43

9 Certification of Grammarian 44

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 ABSTRACT

PASCUAL,JANETH G. (2022). Efficacy of aloe vera extract (Aleo barbadensis)

as Root Enhancer in Dragon Fruit (Hylocereus undatus) Cuttings as Influence
by Different time of soaking. BSA Thesis. College of Agriculture and Fisheries.
South East Asian Institute of Technology, Inc. Tupi, South Cotabato. 41 pp.

Adviser: JOHN BERNARD L. GALLEGO, L.Agr

This study was conducted in Purok 3, Palian Tupi, South Cotabato,

from November 1, 2022, to December 30, 2022. The experiment was carried

out using a Completely Randomized Design with five treatments replicated 4

times each. The treatments were as follows: T1 – No application; T2 – Dipping;

T3 – 6 hours of soaking/400ml of aloe vera extract per treatment; T4 – 8 hours

of soaking/400 ml of aloe vera extract per treatment; T5 – 10 hours of

soaking/400 ml of aloe vera extract per treatment.

The results of the study, based on gathered data regarding the number

of roots, length of roots, number of shoots, number of infected cuttings, and

non-infected cuttings, indicate that there is no significant difference or effect

observed between and across the treatments when using Aloe vera extracts

as a potential rooting hormone in dragon fruit cuttings.

xiii