Version 109.14.

Catalog #: VS-ML-01091-00
SQA-Vision Service Manual Version 109.14.3

Table of SECTION I: Introduction

Contents Automated Test Results 3
Technology 4

SECTION II: System Specifications and Requirements

Sperm Quality Analyzer – SQA-Vision 5

SECTION III: System Overview

Front Panel 7
Rear Panel 7
Side Panel 8
The Chassis Assembly 8
The Cover Assembly 8
Schematic of the SQA-Vision Motherboard 8

SECTION IV: System Components and Accessories

Testing Capillary 9
Cleaning and Accessories Kit 9

SECTION V: Self- Test and Auto-Calibration

Start Up 9
Prior to testing a sample 10

SECTION VI: Getting Started / Set Up

Getting Started / Set Up 10

SECTION VII: Troubleshooting

Overview 11

SECTION VIII: Technical Support and Part Replacement Instructions

Opening the SQA-Vision 11
Closing the SQA-Vision 11
Keypad 12
Power issues 13
Power Supply Unit (PSU) 13
Power Inlet 14
Fan Assembly 14
Operation Monitor (LCD Screen) 15
Focus Knob and Field Of View Knob 18
Surge Protector Replacement 18
Capillary Sensor Troubleshooting and Replacement 19
Camera Replacement 21
Optical Board to LED Cable Replacement 23

SECTION IX: Appendixes

Cleaning the SQA-Vision 24
Replacing the Processor 25
Power Supply Troubleshooting Flowchart 27
Parameters Out Of Range: Troubleshooting 28

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 SQA-Vision Service Manual Version 109.14.3

SECTION I: Introduction
The SQA-Vision is a high-performance PC-based analytical medical device that combines
technology in optoelectronics, computer algorithms and video microscopy. The SQA-Vision and
a fully integrated TOUCH-SCREEN work together to provide user friendly semen analysis testing.
Samples are run in the SQA-Vision testing device and all data entry and user interface is
computer based.
FRESH, POSTVASECTOMY, WASHED, SWIM-UP, DENSITY GRADIENT and FROZEN semen
samples as well as LONGEVITY testing can be performed on the SQA-Vision. Additionally MANUAL
testing can be performed utilizing the on-screen MANUAL COUNTER. The high resolution
visualization component of the SQA-Vision permits assessment of oligospermic specimens and
poor quality IVF samples.
When results fall below the automated dynamic range of the system, a LOW QUALITY COUNTER
opens automatically to permit manual sperm counting. Debris and round cell assessment is
performed using the DEBRIS/ROUND CELLS SCANNER. % Normal morphologically is assessed
automatically along with other semen parameters. Vitality and Morphology differentials can be
assessed manually using the enhanced visualization system and on-line counters.
The analysis time varies according to the type of sample being tested: Normal quality samples –
75 seconds; Low quality – 2 additional minutes; Post vasectomy – 5 minutes.

The system runs a self-test and auto-calibration upon start up. It also runs latex beads or
stabilized sperm external quality controls.

Automated Automated Test Results

Test Results
and
Reportable Concentration M/ml Motile Sperm Concentration (MSC) M/ml
Range
Total Motile Progressively Motile Sperm Concentration
% M/ml
PR+NP (PMSC)
Functional Sperm Concentration (FSC)
Progressive PR % (Progressively motile sperm with normal M/ml
morphology)

Non-progressive mic
% Velocity
NP /sec

Immotile IM % Sperm Motility Index (SMI) #

Normal forms
%
(WHO 5th)

TOTALS PER SEMEN VOLUME

Sperm # M/ejac Functional Sperm M/ejac

Motile Sperm M/ejac Morphologically Normal Sperm M/ejac

Progressively
M/ejac
Motile Sperm

POSTVASECTOMY

Motile, Immotile # Motile, # Immotile and # Total

M/ml M
and Total Sperm Sperm/semen volume

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SQA-Vision Reportable Range (Automated Results)

Motile /
Sperm
Sample Motility Morph MSC PMSC Immotile /
Conc.
Type % % M/ml M/ml Total Sperm
M/ml
M/ml
<0.2 -
Fresh <2 - 400 0 - 100 2 - 30 0 - 400 -
400
<2 - <0.2 - 0-
Washed 0 - 100 2 - 30 -
200+ 200+ 200+
Swim-up,
Density <0.2 - 0-
- - - -
Gradient, 200+ 200+
Frozen
Post-Vas - - - - - 0 - 400

Technology Automated System

Motility Channel

• Light disturbances caused by moving sperm cells are detected and translated into
analog signal peaks.
• The greater the # of motile sperm cells in the field of view, the higher the peak.
• The slower the sperm velocity the broader the signal peaks.
• The average analog signal is mathematically proportional to MSC.

Concentration Channel

• Sperm concentration is measured in the cuvette section of the SQA-testing capillary.

• An infrared light wavelength specific to sperm cells is maximally absorbed by sperm
cells and minimally absorbed by other seminal fluid components.
• In the final calculation of sperm concentration, the SQA-Vision algorithm makes an
adjustment to account for the infrared light absorption of the seminal fluid components.

Motility Self-Test
detector
and QC

Concentration
detector
#4
#3 Analog to Semen
Microcontroller

Digital parameters
#1
#2 Converter extraction
algorithms

Motility
LED

Concentration
LED KeyPad

Operational
Monitor

#1: The capillary is inserted into the measurement compartment.

#2: Sample concentration is evaluated in the "tall" 10 mm chamber of the capillary by
measuring the amount of optical absorption of light as a beam traverses the
seminal fluid.
#3: Motility is detected in the "thin" 0.3 mm section of the capillary by analyzing light
modulations caused by sperm motion.
#4: This information is then digitized and routed to the microprocessor that applies
algorithms to extract the required clinical semen parameters and performs internal
self-testing and calibration.

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SQA-Vision SECTION II: System Specifications and Requirements

Dimensions: 32 X 30 X 24 cm
Weight: 7 Kg
AC power supply: 100-240 VAC, 50-60 Hz, 20 VA

Front Panel
• Displays: LCD display
• Testing: Measurement and Visualization compartments
• Other: Multi-button keypad, Focus knob, FOV (Field Of View) knob
Keypad
• Operational keys: I-Button, Service, Enter, Esc, Delete, four cursor buttons and ten
numeric buttons (0-9).
• Video control key: Zoom In/Out

Measurement Compartment
• Sources of radiant energy - two LEDs for motility and concentration channels
• Detector system - two photo detectors - Motility and Optical Density
Operating System
• Analysis Time: Normal Test – 75 seconds; Low Quality – 2 additional minutes;
Postvasectomy (automated) – 5 minutes
• Software: Resides on flash memory. System can be upgraded from a PC CD-ROM
• Motility channel input signal: Analog, up to 5V.
• Concentration channel input signal: Modulated (kHz) analog, up to 5V.
Rear Panel
• Power inlet w/fuse-holder (fuses: 250V,2A), RS232 connector, 2 USB 2.0 B-Type Male,
On/Off switch of the blue surrounding LEDs (located on bottom side of the panel).
Left Side Panel
• Power On/Off switch
• I-Button port
Visualization Compartment
• White LED illumination system with max luminous intensity – 35,000 mcd.
• Digital Camera, max resolution: 1280 x 1024 pixels, high resolution of "live" and "frozen"
images provided by capturing a high # of FPS (frames per sec).
• Objective: Standard, x40, chromatic aberration correction.
• Zoom system for smooth magnification transition between x1188 and x1725
• Focus adjustment knob
• Field of View Stage knob

SQA-Vision Maintenance Schedule

• Daily: Clean measurement compartment daily when running samples and after every 10-
15 tests and/or for ANY spillage. Follow manufacturer’s cleaning instructions using
manufacturer cleaning kit (Refer to the appendix section “Cleaning the Capillary
Compartment” in this User Guide).
ONLY use the Manufacturer’s cleaning kit and cleaning brush or damage will
occur to the SQA-Vision film and the system will not operate!

Manufacturer Recommendations

• Operate the SQA-Vision away from devices that may cause electronic noise or other
devices causing vibrations such as centrifuges.

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• Turn the system OFF when not in use for an extended period of time.
• When running Postvasectomy tests do not interrupt test cycle nor interfere with system
or testing capillary in any way – this test is highly sensitive to any motion and requires
complete stability of the system during the 5-minute testing cycle.
• Variations in ambient temperature can affect semen sample parameters. It is essential
that semen samples are not heated for testing. The SQA-Vision is calibrated to conduct
tests at room temperature: 20-25ºC (68-77ºF).
• Semen is considered a biologically hazardous material and is subject to
individual laboratory protocols for handling such materials and at a minimum:
• Laboratory coat, mask and gloves for operating personnel protection
• Samples handling and waste disposal in specially marked hazardous waste containers
• Only personnel trained to work with biologically hazardous materials such as semen
should be testing and handling semen
Operating Temperature and Humidity
• Maximum operational humidity is up to 80% for temperatures of up to 31 ºC with
decreasing linearly to 50% at 38ºC.
• Operates in a wide range of ambient temperatures (15-38ºC) however the system is
calibrated to measure semen samples at room temperature:
20-25ºC (68-77ºF). Note: Extreme ambient temperature may impact the accuracy of
motility test results because of the known effect of temperature on human semen.
Operational Environmental conditions:
• System is intended for indoor use at a maximum altitude of 2000m, mains supply
fluctuations ±10%, Overvoltage Category II, Pollution Degree II.

PC / Hardware

• PC and device: "All in One" - computer with SQA-Vision software and device.

Quality Control

• Internal: Electronic Self-Test and Auto-Calibration. Runs automatically upon start-up.

Besides that, reference values are verified prior to each test.
• External: Runs QC samples daily prior to testing or per laboratory protocol. Runs assayed
latex bead control: "QwikCheck™-beads" (product of Medical Electronic Systems) for
concentration and negative control for motility/concentration OR non-assayed: Latex
beads or stabilized sperm CAP or NEQAS for concentration.

Sample Testing

• Sample Testing Temperature: Calibrated for room temperature only. Motility results
will be impacted by heating the specimen (not recommended).
• System calibrated to test Human semen and specified Control samples only. Not
for use with animal semen.
• SQA-Vision measurement capillary: Disposable, plastic, testing capillary. Requires
500 µl of sample for normal volume testing, 20 µl for low volume testing, 300 µl for diluted
mode. Use only manufacturers’ certified testing capillaries in the automated system.
• Field of View Stage: This stage is an integral part of the SQA-Vision visualization
compartment. Vision™ fixed cover slip slides (obligatory for accurate test results when
manual sperm counting is required), standard slides (for debris scans and image
capturing) are accepted in the field of view stage using the supplied slide holder.

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SQA-Vision SECTION III: System Overview

Overview
Front Panel

Visualization
compartment accepts
either fixed coverslip
Operation Display
slides or standard
slides (Zoom
magnification
1188X÷1725X)
Keypad

Field of View Knob

Power indication LED
Focus Knob

I-Button Automated
Measurement
Compartment

Keypad Navigation

• Use NUMERIC keys to enter data; ARROW keys to move to the next field.

• Press ENTER to select menu options, confirm data entries and to move to the next screen
or field.

• Use the ESC button to return to the previous screen or field.

Rear Panel Rear Panel

Rear Panel
Assembly Screws
(x4)

Ventilation Slots
RS232 COM Port

USB Data Port Power Inlet

USB Video Port Product Label

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Side Panel Side Panel

• The side panel has an I-Button port with
spring clip
(Refer to the Appendix instructions on how to
load I-button tests)
• The side panel also includes the On/Off switch On/Off switch I-Button

Assemblies The Chassis Assembly The Cover Assembly

The chassis assembly includes both the The cover assembly includes the following:
base and rear panel and contains the
following sub-assemblies:
• Operational Display

• Main Board
• Keypad

• Switch Power Supply

• Power Indicator LED

• USB outlets
• Visualization Compartment

• Surge Protector AC Power Inlet

• Measurement Compartment
Assembly • Focus Control
• Ventilation Fan • Field of View control
• RS232 Communication port

Motherboard Schematic of the SQA-Vision Motherboard

Schematic Fuse Surge
Main switch 2A x 2 protector Notes:

AC
100-230 V,
Keypad SQA-Vision video
50/60 Hz camera receives the
GND 1 2 3 power from PC s USB
4 5 6 Vision video port and doesn t have
camera USB a connection to
CN1 7 8 9
2.0 device s Motherboard
Chassis Main F 0 .

Power Supply
CN2
+12 VDC
FAN +5 VDC Decorative LEDs
-12 VDC
+12 VDC

+12 VDC
JP1 J11 J8
Switch RS232 Port
I-Button Port J18
J5
+2 VDC
On/Off LED J14 Mother Board
USB Data Port
+5 VDC
+3 VDC
Visualization LED J1
J10 +5 VDC
J1 J2
J17 J13 J7 -13 VDC
Operational
-16 VDC
Backlight PCB Monitor

J3 Backlight LEDs
+5 VDC +3.5 VDC
+12 VDC

DC Motor
JP1
Capillary Optical Board
sensor LED s Board
J2 JP5
J1 JP6
+1.7 VDC
+1.5 to +1.8 VDC
+1.5 to +1.8 VDC

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SECTION IV: System Components and Accessories

Testing Testing Capillary
Capillary

• Can be used in both measurement chambers of the SQA-Vision.

• Disposable; designed to collect and test samples in a biologically safe manner.
• Motility is measured in the 0.3 mm (thin) "Capillary Section."
• Concentration is measured in the 10 mm (tall) "Cuvette Section."
Cleaning Kit Cleaning Kit
• Daily cleaning is recommended or after testing 10-
15 specimens.
• See the detailed cleaning instructions in the
Appendix Section.
• Note: Only use the manufacturer’s cleaning kit or
the SQA-Vision can be damaged.

Accessories Kit Accessories Kit

• Each kit contains:
• Power Cables
• Communication Cable
• USB cables
• RS232 cable
• RS232 to USB converter
• Instructional Guides
• Slide holder
• Additional set of device feet
• Barcode reader
• Capillary piston – Opening and Removal Jig

Self-Test and Section V: Self-Test and Auto Calibration

Auto-
Calibration The SQA-Vision automatically runs a series of tests to check calibration settings and the
internal operating system. Tests are run when the system is turned on and prior to
testing a sample.

Start-up Start-up:

• Stabilization and autocalibration: Checks system stability and reference parameters.

The system sensors are analyzed for several minutes to ensure that the reference
parameters are within acceptable ranges. Once the system is stable for 30 seconds it will
pass stabilization and autocalibration. A warning message of the system stabilization

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failure will be displayed if it is not stable for at least 30 seconds and the reference
parameters are not within acceptable ranges.
• System noise: Measures the electronic noise level of the system to insure effective
measurement of electronic signals.
• Self-test: The system produces electronic signals that simulate motility and concentration
measurements in order to check the performance of the system and verify that the
calibration settings are consistent with the factory specifications. The SQA-Vision will
report the Self-Test failure (see section on error and warning messages) if the system is
not within the established Self-Test ranges.
Prior to testing a sample:

• Autocalibration verification: Reference parameters of the concentration and motility

channels are measured again (without a testing capillary).
• System noise: Measures the electronic noise level of the system to insure effective
measurement of electronic signals. Prior to running a test, the SQA-Vision will
automatically adjust the noise level threshold to insure accurate readings.
• Electronic spikes: Checks for measurement points that are out of range electronically
and displays a warning message if out of range.

Service Instructions for printing the SQA-Vision SERVICE parameters to prepare for technical
Parameters support:

• If a Self-Test failure occurs, the related status icon in the HOME screen will turn red:

• Click: SELF-TEST STATUS icon to open a warning message with instruction on how to
resolve the problem:

• Click: REPORT from the HOME or SERVICE screen to generate a SERVICE REPORT.
This may be required for troubleshooting and technical support.

SECTION VI: Getting Started / Set-Up

(Refer to the User Guide)

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Troubleshooting SECTION VII: Troubleshooting

Overview: The following sections describe how to troubleshoot and repair potential problems
that may occur with the SQA-Vision. Theses section will discuss how to provide support for
three types of issues: Technical - Self test - Clinical

PLEASE NOTE:

• Only a qualified, technical support trained MES distributor is authorized to open

the SQA-Vision.
• If the SQA-Vision is opened without authorization it may cause damage to the
calibration AND will VOID THE WARRANTY.
• The electro-optical board should NEVER be touched when the SQA-Vision is
opened – it will cause damage to the system’s calibration.

Technical SECTION VIII: Technical Support/Part Replacement

Support and Opening the SQA-Vision
Part
• Turn off the main switch located on the side panel and
Replacement disconnect the SQA-Vision from the electrical supply.
• Using a Philips screwdriver, remove
all screws on the rear panel.

Closing the SQA-Vision

• Grip each panel from the side and gently push them
back together making sure that all cables and
connectors are free and not “pinched” between the
panels.
• Use a standard Phillips screwdriver to reconnect the
panels.

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Keypad Keypad (Part # VS-E-00851-00)

ISSUE: The Keypad is not working
• Open the SQA-Vision
• Verify that keypad cable is connected firmly to
connector J11 on the Main board (connector is not
loose)
• Confirm that the cable is connected in the correct
orientation: The golden wide stripe on the cable
connector should be facing the rear panel
• If the cable is connected properly and the keypad is still
not working- replace the keypad:
• Disconnect the keypad cable connector from location
Verify connection of
Keypad
J11 on the Main board
• Using a flat screwdriver, carefully peel off the damaged
keypad from the front panel. Clean the surface with
alcohol and let it dry.
• Pull the keypad cable through slit in the front panel

Please note: DO NOT connect it currently.

• Attach the keypad to its designated location on the
front panel by gradually removing the paper while
pressing it firmly into place from one side to the other. Peel off the damaged
Please note: DO NOT bend the keypad during this Keypad
process or electrical wires/connectors will be
damaged.
• Attach the keypad connector to the J11 location on the
main board.
Please note: The golden stripe on the cable connector
should face the rear panel (the fan side).
Close the SQA-Vision using 4 screws on the rear panel of the Attach the NEW Keyboard
device. Connector

Apply the New Keypad

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Power Power issues - fuses, PSU, power inlet.

issues: fuses, ISSUE: The main switch is ON, but the power indicator
PSU, power does not light up and the fan is not working
inlet
• Check the fuses in the fuses box located on the rear
panel of the SQA-Vision.
Notes:
1. Turn off the • Replace the fuse if it is burned out.
power supply
and disconnect • Reconnect the power cord and turn the unit back on.
the power
supply cable
• If the unit still does not work, check the input/output Fuses box
from the back of
voltage of PSU as instructed below.
the SQA-Vision
before opening.
2. Refer to the How to check the input voltage of PSU:
Appendix section
for instructions
• Check the voltage on the input connector of the main
on how to PSU using a multimeter
address power • The voltage should be ≈220V AC between the
supply problems. black and the red wires

Note:
• If no voltage is evident, replace the power inlet (see
Before checking the relevant section below)
PSU Input connector
the input\ output
connectors of the
• If voltage is being supplied to the input connector,
check the output connector.
main PSU, turn on
the SQA-Vision.
How to check the output voltage of PSU:
• Disconnect the output cable from the motherboard.
• Check the voltage on the output connector of the main
PSU using a multimeter:
• 0V: black wire
• +5V: red wire
• +12V: purple wire
• -12V: white wire
Power Supply Output Cable
• If there is no voltage on the output connector or the
voltage is not correct, please replace the main PSU as
instructed below
• If the problem persists, please contact Customers
Support.
Power Supply Replacing the Power Supply Unit –
Unit (PSU) part #Fpe-E-00146-00
ISSUE: The power supply and/or cables are not working
• Open the SQA-Vision.
• Disconnect the input connector of the power supply
unit- location CN1. Unscrew 4 screws of PSU

• Disconnect the output connector of the power supply

unit- location CN2.
• Using a #2 Philips screwdriver, unscrew the four
screws that connect the PSU to the rear panel.
• Remove the old PSU.
• Secure a new PSU to the rear panel using the same
four screws.
• Re-connect the input and output cable connectors to
Power Supply Unit
the PSU.

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 SQA-Vision Service Manual Version 109.14.3

• Close the SQA-Vision. Reconnect the power cord and

turn the unit back on. If the problem persists -
Contact Customers Support

Power Inlet Power Inlet (Part# VS-E-00850-00)

ISSUE: There is no voltage supplied to the PSU from the inlet.

Notes: • Open the SQA-Vision.

1. Turn off the
On/Off switch and
• Unscrew the power-inlet screws at the rear panel of the device.
disconnect the • Disconnect the power supply connector from the power supply board.
power cord from
the SQA-Vision • Gently pull the rubber cover of the power inlet until the connectors are exposed.
before opening.
• Please note how all connector are connected to the power inlet.
2. Refer to the • Disconnect all connectors from the power inlet.
Appendix section
for a flow chart of • Gently pull the power inlet out of the SQA-Vision through the rear panel.
power supply
problems and • Insert a new power inlet and re-connect the cables.
solutions.
• Re-connect the internal cables, re-place the internal cover and re-connect the power
supply cable to the power supply board.

Unscrew the power inlet Gently pull the power inlet

screws Gently pull the rubber cover from the rear panel

Fan Assembly Fan Assembly (part # V-H-00575-00)

ISSUE: The master switch is ON, the power indicator is lit but the fan is not rotating
• Open the SQA-Vision.
• Confirm that all the fan connecting cables are in place.
• Check that the voltage in the main board connector of
the fan (location JP1) is:
• 0V: black wire
• +12V: purple wire
• If no power is evident, replace the main PSU (see
previous section)
• If the voltage is as described above, replace the fan:
• Unscrew the four fan screws. Fan Assembly
• Replace the fan assembly (fan + cable).
• Re-connect the cables and screw the new fan to the rear panel of the SQA-Vision.
• Do not change the direction of the outlet air flow! (the arrow on the fan should point
outwards)

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Operation Operation Monitor - Lower LCD Screen (Part# LCD-0009)

Monitor ISSUE #1: The SQA-Vision is ON, both power indicators are functioning and the fan is
working. But the LCD screen (Operation Monitor) is not illuminated although data is displayed
on the screen.

PLEASE NOTE: The instructions depend on the SERIAL NUMBER (SN#) of the SQA-V.
• SN# 5228 and below – follow the instructions in SECTION 1
• SN# 5229 and above – follow the instructions in SECTION 2
SECTION 1: • Open the SQA-Vision.
SN #5228 • Turn on the SQA-Vision and check that the
and below LCD Backlight is lit. If not, check the input
and output cables of the inverter board:
Note: Turn off verify that the cables are well connected and
the power
not loose.
supply to the
SQA-Vision • If the power supply is OK and the screen
and disconnect doesn’t light up, replace the inverter board
the power (Item#AS-9084111):
supply cable
from the back
• Turn the SQA-Vision off and disconnect Inverter Board Backlight Lamp
of the device
the cables connecting the inverter board
before opening to the main board and LCD screen.
the SQA-V. • Using a Phillips screwdriver, remove the
two screws that secure the old inverter
WARNING: board.
Do not touch the
backlight lamp and • Replace the inverter board with a new
inverter board one and secure it with the two screws.
while SQA-Vision is
on! There is a risk • Re-connect the cables of the inverter
of HIGH VOLTAGE board.
shock!
• If the problem persists, contact MES
Cables connecting the inverter board
Customer Support.
to the main board and LCD screen

SECTION 2: • Open the SQA-Vision.

SN #5229 • Turn on the SQA-Vision and check that the Data Cables LCD Screen
and above LCD Screen is lit. If not, check the input and
output cables of the Backlight PCB: verify
Note: Turn off that the cables are well connected and not
the power loose.
supply to the
SQA-Vision • If the power supply is OK and the screen
and disconnect doesn’t light up, replace the long flat cable
the power connects the main board and the Backlight
supply cable PCB. (Item #KHD-908-000858)
from the back
of the device
• If the screen doesn’t light up after changing
before opening the long flat cable, replace the Backlight PCB
the SQA- (Item#V-B-01410-00):
Vision. • Turn the SQA-Vision off and disconnect
the cables connecting the Backlight PCB
WARNING: to the main board and LCD screen.
Do not touch the
backlight lamp and Power Cable Backlight PCB
inverter board
while SQA-Vision is
on! There is a risk • Using a Phillips screwdriver, remove the two screws that secure the old Backlight
of HIGH VOLTAGE PCB.
shock!
• Replace the Backlight PCB with a new one and secure it with the two screws.
• Re-connect the cables of the Backlight PCB.
• If the problem persists, contact MES Customer Support.

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SECTION 1: ISSUE #2- Blank Screen:

SN #5228
and below There is no data displayed on the screen although the SQA-Vision is ON, both power indicators
are functioning and the fan is working.
PLEASE NOTE: The instructions depend on the SERIAL NUMBER (SN#) of the SQA-V.
WARNING: • SN# 5228 and below – follow the instructions in SECTION 1
The two ends of
the Data flat cable • SN# 5229 and above – follow the instructions in SECTION 2
must be connected
in the same way at
• Re-install SQA-Vision software.
each of the nodes • If the software was not installed successfully- please refer to the technical bulletin in the
(Operation appendix section for further instructions
monitor,
Motherboard) or
• If the software was installed successfully and the problem remains- check the Data flat
the LCD may be cable:
burned! • Open the SQA-Vision and verify that the Data flat cable, with the red lined side up is
connected to the section designated with a 12 on the main board
• Replace the flat cable if it appears damaged in any way.
• If replacing the cable does not work- replace the processor on the main board (see
Appendix section for instructions).

LCD Operation Note the alignment of the red line on the Data
Data Cable cables
Monitor/Board

• If replacing the processor does not work:

• Re-start the SQA-Vision and see if the LCD Operation Monitor is still blank. If yes, replace
the screen:
• Disconnect the Operation Monitor Data and Backlight lamp cable
• Unscrew the four screws.
• Replace the screen & reconnect the Data and Backlight lamp cables.
• In case the problem persists after replacing the LCD screen- contact MES Customer
Support.

Data Cable

Backlight lamp cable Unscrew the four screws

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SECTION 2: • Re-install SQA-Vision software.

SN #5229
and above • If the software was installed successfully but the problem remains - check the LCD flat
cable:
• MB side: Open the SQA-Vision and verify that the LCD flat cable is oriented with
WARNING: the red lined side toward J1 connector (as shown in the picture below).
The two ends of
the Data flat cable • Replace the long flat cable which connects the main board and the Backlight PCB.
must be connected (Item #KHD-908-000858)
in the same way at • If replacing the long flat cable does not work – Replace the short flat cable which
each of the nodes connects the LCD screen and the Backlight PCB. (Item#V-H-01411-00)
(Operation
monitor, Backlight • If replacing the short flat cable does not work - Replace backlight PCB.
PCB and (Item#V-B-01410-00)
Motherboard) or
the LCD may be
burned! Note the alignment of the
red line on the Data cable

Note: For more

detailed
explanations
regarding blank
screen issues,
please refer to the
“blank screen
technical bulletin”
in the appendix
section

• If replacing the backlight PCB does not work - replace the processor on the main board
(see Appendix section for instructions)
• If replacing the processor does not work, re-start the SQA-Vision and see if the LCD
operational screen is still blank. If yes, replace the screen:
• Disconnect the operational display data and power cable - note the four screws.
• Replace the screen & reconnect the data and power cables

• If the problem persists with the new screen – perform an MBOB replacement OR
send the SQA-Vision back to the manufacturer (MES) for a repair RMA

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 SQA-Vision Service Manual Version 109.14.3

Focus Knob Focus knob (Part# PE-MA-00538-00)

Please be
advised:
ISSUE: When using the focus knob there is no change in the visualization system (the focus
Only an shown on the PC screen is not changed).
experienced
and licensed
• Open the SQA-VISION.
electrical • Unscrew the focus knob cover
technician or • Pull out the focus knob base.
electrician
should perform • Disconnect the optical assembly from the front panel (five screws).
this repair. • Disconnect the focus knob from the optical assembly by unscrewing three screws
• Remove the old focus knob
• Insert the new focus knob making sure it is turned all the way counterclockwise before
replacing
• Re-connect the optical assembly to the front panel and close the SQA-VISION

Disconnect the Replace the

focus knob New focus knob
focus knob

Field of Field Of View Knob (Part# VS-M-01105-00)

View Knob ISSUE: When using the FOV knob there is no change in the field of view.
SOLUTIONS: This is described in a separate technical bulletin- please contact MES support
services for further instructions.

Surge Surge Protector Replacement (PN: V-A-00475-01)

Protector Electrical surges or high voltage spikes in mains may cause damage to the SQA-Vision surge
protector. Follow below instructions to check if the surge protector is intact or to replace the
damaged surge protector to prevent further damage of SQA-Vision internal components.

How to check the Surge Protector:

• Turn off the power and unplug the power cord from
the inlet on rear panel of SQA-Vision
• Open the SQA-Vision
• Look through 3 small windows located on the front
face of the surge protector and verify that the
surface inside the windows is green
If at least one of the surfaces inside the windows is not
green, replace the surge protector

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SQA-Vision Service Manual Version 109.14.3

How to replace the Surge Protector:

• Follow the instructions from the section above to open the SQA-Vision
• In order to remove the defective Surge Protector all its 3 wires should be released
• Insert a small flat screwdriver into square opening in PDP (tri-colored contact block)
right below the wire
• Simultaneously gently push down the screwdriver in order to release the wire and pull
out the released wire
• Release and pull out from the PDP all 3 wires of Surge Protector (2 black and 1 yellow-
green)
• Cut all tie wraps attaching the wires to the rear panel
• Using 5.5mm socket screwdriver unscrew the nut connecting the Surge Protector to the
rear panel
• Replace the defective Surge Protector with the new one and reconnect all its wires back
to the PDP

Capillary Capillary Sensor Troubleshooting and Replacement (KHD-908-000846 REV 02)

Sensor Issue: When the SQA-Vision is turned on, it fails SELF-TEST
• Ensure there is no testing capillary in the measurement compartment
• Remove the SQA-Vision from sources of electronic noise (cell phones, etc.)
• Clean the measurement compartment per User Guide instructions
• Reboot the SQA-Vision without a testing capillary in the chamber:
• Turn the system off then back on at the main switch on the side panel.
• If the self- test continues to fail after the reboot do the following:
• Print a copy of the SELF- TEST parameters
• Check parameter #17. This parameter represents the noise level. If the value is
higher than 3- the noise level is too high. In this case replace the cables which
connect the Optical Board to the LED Board
• Check the values of Conc 1, Conc 2 and Conc. 3. If these values are ZERO, the
capillary sensor may be damaged and will need to be replaced
• Turn off the power and open the SQA-Vision by unscrewing the screws from the rear panel
using a #2 Philips screwdriver
• Pull out the focus knob
• Using a #2.5 Allen key, remove the five screws connecting the optical column to the front
panel of the SQA-Vision

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SQA-Vision Service Manual Version 109.14.3

• Carefully pull the optical assembly to gently release it from the front panel
• Place the optical assembly on its side
• Release the 4 screws holding the Dust Buster using a #2 Philips screwdriver
• Release the screw holding the capillary sensor using a #2 Philips screwdriver

Release the Capillary

Release the 4 screws of Sensor Screw
Dust Buster
Pull the Optical
Assembly

• Unwind the white plastic cable wrap that holds the capillary sensor and other cables
• Remove the damaged capillary sensor from the optical block and unplug the cable
connector from its location on the main board - J13
• Attach a new capillary sensor to the optical block using a screw.
• Gently re-attach the Dust Buster to the Optical Block using the 4 Phillips screws.
• Re-attach the cable to the cable bundle, fasten the cables using the white plastic cable
wrap.
• Connect the cable connector to its designated location on the main Board- J13.
• Attach the optical column to the front panel of the SQA-Vision using the original 4 screws.
• Close the back the front and rear panels of the SQA-Vision using a #2 Philips screwdriver.

Unwind the Plastic Connector J13

Cable Wrap New Capillary
Sensor

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SQA-Vision Service Manual Version 109.14.3

CCD Camera Replacement (Part # VS-MA-01174-00)

Sensor • Issue: When the camera is dirty or malfunctioning, then the CCD sensor including the
assembly of the mount and the dust sleeve should be replaced.
• Turn off the power and open the SQA-Vision by unscrewing the screws from the rear
section of the rear panel using a #2 Philips screwdriver.
• Pull out the focus knob.
• Disconnect the optical assembly from the front panel by unscrewing 5 screws using a #2.5
Allen Key.

• Release the screws at the rear section of the optical column

using a #2.5 Allen key
• Release the zoom DC motor by unscrewing 2 screws using a
#2.5 Allen Key

Remove the screws

Release motor Remove the DC motor at the rear section
assembly screws assembly

• Release the USB protector and the bottom cover by unscrewing 4 screws using a #2.5
Allen Key

Remove the USB protector and the bottom cover

• Release the camera USB stopper by unscrewing 2 screws using #0 Phillips screwdriver

Remove the USB stopper and disconnect

the camera USB cable

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 SQA-Vision Service Manual Version 109.14.3

• Release the stopper from the bench at the bottom of the optical column by unscrewing 2
screws using a #2 Allen Key
• Line up the false track with the steel rail

Lining up the false

Remove the Camera stopper track
Note:
The linear slide • Make sure that the false track is pressed securely against the
contains many steel rail in order to avoid dislodging the small bearing balls
small bearing balls
that can be
• Slide the old camera tray over false track and seal it with the
dislodged unless a two plastic stoppers to prevent the tray from slipping out
false track is
used to secure
them. Slide the old Camera tray
over the false track

• Remove the front plastic stopper

from the new camera assembly
• Slide the entire new camera
assembly back into the optical
assembly over the false track

Remove the front plastic

stopper Slide the new camera
assembly back

• Re-attach the Camera Stopper

• Reassemble the optical assembly by screwing the four screws on the rear section of the
optical column
• Reconnect the USB Camera cable connector and put it back into place
• Reattach the base of the optical column on the assembly
• Reattach the zoom DC motor, and make sure the cogwheel is matched with the teeth of
the white plastic rack
• Reattach the optical column to the front panel
• Reinstall the focus knob on the designated axis
• Ship to MES both false tracks, the old camera assembly and the empty one in coordination
with the customer support department

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 SQA-Vision Service Manual Version 109.14.3

Optical Optical Board to LED Cables - Replacement

Board (Part # KHD-908-000689 - Rev. 01)
Cables Issue: When the noise level of the SQA-Vision (Parameter
Note: #17) is higher than 3, then the cables connecting the Optical
The following Board to the LED Board need to be replaced.
instructions for
replacing the
• Turn off the power and open the SQA-Vision by unscrewing
Optical Board the screws from the rear panel using a #2 Philips
cables are screwdriver.
applicable only for • Pull out the focus knob.
devices where
aforementioned Open the SQA-Vision
cables are joined
with the boards by • Using a #2.5 Allen Key, release the 5 screws which hold the Optical column to the front
means of panel (shown in the pictures below)
connectors.
For those devices
where the Optical
Board cables are
joined with the
boards using
soldering method
the following
instructions are
not applicable!
Please contact the
MES customer
support team for
appropriate
• Remove the damaged cables from the Optical board by
solution. disconnecting the J5 and J6 connectors
• Carefully lay down the Optical Base
• Remove the damaged cables: disconnect the J1 and J2
connectors from their locations on the LED board
• Connect a new pair of cables as follows:

• Connect the cables back to the J1 and J2

connectors on the LED Board
• Connect the cables back to the J5 and J6 Disconnect the J5 and
J6 connectors
connectors on the Optical Board
Important Note: Pay close attention to connect the cables
in the correct direction (from J1 to JP6 and from J2 to JP5)

• Reattach the optical column to the front panel using the

same 5 screws
• Close the SQA-Vision.

Disconnect the J1 and

J2 connectors

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 SQA-Vision Service Manual Version 109.14.3

SECTION IX: Appendixes

Appendix 1:
SQA Cleaning
Instructions

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 SQA-Vision Service Manual Version 109.14.3

Appendix 2: Instructions for RE-SEATING or REPLACING the SQA-Vision PROCESSOR

Replacing the Applies to all SQA-Vision system versions
Processor
Stage 1: Re-seating the processor
to the correct position
1. Turn off the SQA-Vision and
disconnect the power supply
cable
2. Loose the 4 screws on the rear
panel using a Philips
screwdriver #2 and open the
SQA-Vision

Open the SQA-Vision

3. Slightly press the center of the

processor with an index finger
to re-seat it into the correct
position
4. Close the SQA-Vision and
tighten all screws of the rear
panel
5. Connect the power cable of the
SQA-Vision
6. Turn the SQA-Vision on and run
the SELF-TEST
7. If the SQA-Vision passes, the
repair process is complete Slightly press the center of
8. If the SQA-Vision does not the processor
turn-on or fails the self- test,
go to Stage 2
Stage 2: Replace the damaged
processor
1. Turn off the SQA-Vision and
disconnect the power supply
cable
2. Release the 4 screws on the
rear panel using a Philips
screwdriver #2 and open the
SQA-Vision
3. Remove the damaged
processor using extractor
tweezers as shown on the
figure to the right
Remove the damaged
processor

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SQA-Vision Service Manual Version 109.14.3

4. Replace the damaged processor

with a new processor according
to the following directions:
Align the “dot” which is marked
on the processor with the
“Arrow” mark on the processor
socket (see the figure to the
right)

Dot mark Arrow mark

5. Slightly press the center of the

processor with an index finger
to re-seat it into the correct
position (see the figure to the
right)

Slightly press the center of

the processor

6. Close the SQA-Vision and tighten all screws of the rear panel
7. Connect the power cable of the SQA-Vision
8. Turn on the SQA-Vision and verify that it successfully passes the SELF-TEST

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SQA-Vision Service Manual Version 109.14.3

Appendix 3: Power Supply – Troubleshooting Flow Chart

The main switch is ON, but the

power indicator does not light up
and the fan is not working

Burnt fuses Check the fuses Fuses are OK

(see p.13)

Replace the fuses

(see p.13)

Yes Not
Problem was
solved?

Check the input

voltage of PSU
(see p.13)
End of
troubleshooting

Check the output OK Not OK Replace the

voltage of PSU Voltage is OK? power inlet
(see p.13) (see p.14)

OK Not OK Yes Problem was Not

Voltage is OK?
solved?

Contact MES Replace the Contact MES

support PSU support
services (see p.13-14) services

End of
troubleshooting

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 SQA-Vision Service Manual Version 109.14.3

Appendix 4: Parameters: Description and Troubleshooting Guide

SELF-TEST Parameters

Acceptable Describing and Troubleshooting

Parameter
Range Parameters Out of Range (High or Low)

REFERENCE
1 (REF 1) REFERENCE 1 PASSED THE SELF-TEST
150 – 350 mV
ACCEPTABLE RANGE: 150mV – 350mV

REFERENCE REFERENCE 1 FAILED THE SELF-TEST

1 (REF 1) If the REFERENCE 1 (REF 1) is not within the acceptable range of 150-350mV
150 – 350 mV it indicates an:
• Optical board problem. New motherboard/optical block, “MBOB”
required (RMA)

LED
CURRENT 1
LED CURRENT 1 PASSED THE SELF-TEST
(LED 1) 5 – 20 mA
ACCEPTABLE RANGE: 5mA – 20mA

LED CURRENT 1 FAILED THE SELF-TEST

LED If the LED CURRENT 1 (LED 1) is not within the acceptable range of 5-20mA M
CURRENT 1 and is not identical to the initial manufacturer’s setting (you must compare) O
(LED 1) it indicates the following: T
5 – 20 mA • High vs. Initial Setting: Dirt on motility channel (CLEAN)
I
L
• Low vs. Initial Setting: Scratched film requiring a new I
motherboard/optical block, “MBOB” (RMA required) T
• Optical board problem if not resolved Y

Amplitude
AMPLITUDE PASSED THE SELF-TEST
50 - 100 mV
ACCEPTABLE RANGE: 50mV – 100mV

AMPLITUDE FAILED THE SELF-TEST

Amplitude If the AMPLITUDE is not within the acceptable range of 50-100mV it indicates
the following:
50 – 100 mV • High: Scratched film requiring a new motherboard/optical block,
“MBOB” (RMA required)
• Low: Dirt on the motility channel (CLEAN)
• Optical board problem if not resolved

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 SQA-Vision Service Manual Version 109.14.3

Acceptable Describing and Troubleshooting

Parameter
Range Parameters Out of Range (High or Low)

Zero Level
ZERO LEVEL PASSED THE SELF-TEST
500 – 525
ACCEPTABLE RANGE: 500 - 525

ZERO LEVEL FAILED THE SELF-TEST

If the ZERO LEVEL is not within the acceptable range of 500-525 it indicates
the following:
High:
Zero Level
• The light source is obstructed with dirt or debris (CLEAN)
500 – 525 • Ambient temperature is below 20 oC / 68oF (maintain room
temperature between 20-25oC / 68-77oF per user guide)
Low:
• The light source is obstructed with dirt or debris (CLEAN)
• Ambient temperature is above 25 oC / 77oF (maintain room
temperature between 20-25oC per user guide)
Optical board problem if not resolved

REFERENCE
2 (REF 2) REFERENCE 2 PASSED THE SELF-TEST
2500 – 3500
ACCEPTABLE RANGE: 2,500mV – 3,500mV

REFERENCE 2 FAILED THE SELF-TEST

If the REFERENCE 2 (REF 2) is not within the acceptable range of 2500-
3500mV it indicates the following:
REFERENCE
2 (REF 2) Low: The light source is obstructed with dirt or debris (CLEAN)
High: Optical board problem. New motherboard/optical block, “MBOB”
2500 – 3500 required (RMA)
C
O
REF2=0: Damaged Optical board to LED board cables/ Optical board problem
N
• Replace cables in the field C
• Optical board problem. New motherboard/optical block, “MBOB” E
required (RMA) N
T
REFERENCE REFERENCE 2 PASSED THE SELF-TEST
2 (REF 2) R
BUT IS NOT OPTIMAL
2800 - 3200 A
OPTIMAL RANGE: 2,800mV – 3,200mV
(CLEAN) T
I
LED O
CURRENT 2 N
(LED 2) LED CURRENT 2 PASSED THE SELF-TEST
10 – 32 mA
ACCEPTABLE RANGE: 10mA – 32mA

LED
LED CURRENT 2 FAILED THE SELF-TEST
CURRENT 2
If the LED CURRENT 2 (LED 2) is not within the acceptable range of 10-32mA
(LED 2)
10 – 32 mA and is not identical to the initial manufacturer’s setting (you must compare)
it indicates an optical board or LED problem requiring a new motherboard
/optical block, “MBOB” (RMA)

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 SQA-Vision Service Manual Version 109.14.3

Describing and Troubleshooting

Parameter
Parameters Out of Range

Auto-calibration and
stabilization AUTO-CALIBRATION AND STABILIZATION PASSED

ZERO LEVEL parameter is stable

AUTO-CALIBRATION AND STABILIZATION FAILED

If the auto calibration and stabilization process fails, the following steps should be
taken:
Auto-calibration and
stabilization • Remove testing capillary from the measurement compartment.

• Remove the SQA-Vision from sources of vibrations (centrifuge)

• Clean the measurement compartment

• Reboot the SQA-Vision without a testing capillary in the chamber: Turn

the SQA-Vision OFF then back ON

• Call technical support if failure recurs

Self-Test
SELF-TEST PASSED
The key system parameters are in range

CONTROL LEVEL TEST PASSED

Control Level 1\2\ 3\ negative
The control level 1 OR control level 2 OR control level 3 OR negative control is
within the acceptable range (as specified on the controls box)

Control Level 1\2\ 3\ negative

CONTROL LEVEL TEST STATUS IS NOT DEFINED
If there is a probability that the control test result is out of range. For example,
the control test result is CONC. < 2.0 (M/ml) and the acceptable range is 0.0

CONTROL LEVEL TEST FAILED

If the control level 1 OR control level 2 OR control level 3 OR negative control is
Control Level 1\2\ 3\ negative not within the acceptable range (as specified on the controls box), the
the following steps should be taken:
• Make sure to mix the controls before testing
• Check the expiration date of the controls
• If expired, run a new box of controls
• Call for technical assistance if controls are still out of range

I-Button tests remaining

I-BUTTON TESTS REMAINING: > 10
There is more than 10 I-Button tests remaining in the system

I-Button tests remaining

I-BUTTON TESTS REMAINING: < 10
I-Button tests should be added to the system

I-Button tests remaining I-BUTTON TESTS REMAINING: 0

In order to perform automated tests, I-Button tests should be added to the
system

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SQA-Vision Service Manual Version 109.14.3

Next Backup
A SYSTEM BACKUP NEEDS TO BE PERFORMED
The system backup was not performed yet. Open the Vision Service screen (PC)

MESSQAV.mdf FILE WAS NOT FOUND IN SQA-VISION’S LOCAL PATH

Last Backup
Perform the following actions:
• Make sure that MESSQAV.mdf file is closed
• Restart the PC
Call for technical assistance if the problem remains

ERROR DURING BACKUP PROCESS

Last Backup
The following actions should be taken:
• Make sure there is enough space on HDD for the backup file to be saved
• MESSQAV.mdf file is closed properly
Call for technical assistance if the problem remains

Last Backup BACKUP DESCRIPTION FILE WAS NOT SAVED

THIS ERROR DOES NOT CAUSE THE BACKUP PROCESS TO FAIL, SO YOU
MAY IGNORE IT

Last Backup
BACKUP PROCESS HAS BEEN FINISHED SUCCESSFULLY
The relevant MESSQAV.mdf file is saved in the defined backup location

HDD Space Used THERE IS STILL HDD SPACE REMAINING

(USAGE < 80 %)
The HDD free space is enough for the system optimal performance

HDD Space Used

IT IS RECOMMENDED THAT YOU FREE SOME HDD SPACE
DELETE UNUSED FILES

HDD Space Used THE HDD IS OUT OF SPACE

PLEASE DELETE UNUSED FILES TO FREE-UP SPACE
The HDD free space is not enough for the system optimal performance. The free
space can be enlarged by unused files deletion

31