Low Speed Centrifuge

BKC-TL6III
User Manual

BIOBASE GROUP

Version 2020.09
 Content
IMPORTANT !!......................................................................................................................................3
1. Safety Caution.................................................................................................................................... 4
1.1 Attention in using...................................................................................................................... 4
1.1.1 Before connecting power.................................................................................................4
1.1.2 In the processing..............................................................................................................4
1.1.3 After using....................................................................................................................... 4
1.2 Attention in operating environment...........................................................................................4
1.3 Transport and storage conditions...............................................................................................4
2. Main performance indices.................................................................................................................. 5
3. Working principle...............................................................................................................................6
4. Structure diagram and system chart................................................................................................... 7
4.1 Structure Diagram..................................................................................................................... 7
4.2 System Chart.................................................................................................................................... 8
5. Installation and Using.........................................................................................................................9
5.1 Environment requirements........................................................................................................ 9
5.2 Rotors Assemble System........................................................................................................... 9
5.3 Control system instructions....................................................................................................... 9
5.3.1 The control panel.............................................................................................................9
5.3.2 Operating instructions..................................................................................................... 9
6. Common fault analysis & removal...................................................................................................12
7. Repair and Maintenance................................................................................................................13
8. Special tips and disclaimer............................................................................................................... 14

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 IMPORTANT !!
Checking before running
● You should confirm that the gland nut is tight before start.
● When the instrument in using, Single phase outlet to make sure it grounded firmly.
● When it in working,it should ensure that its working voltage within the prescribed scope. If the
instrument can not work normally, please check the power supply firstly.
● To ensure the safty of the instruments and the operator, the setting value speed should less than the
max speed when it in running.
● After power off, please wait more than 3 mins and then open the power.
● Please loading the four cups in the same time when the instruments in running,The quality
devistion of centrifugal tube with the samples should be less than 5g, and must be symmetricalin the
rotors. Two ear line of cups should towards the center of motor shaft.
● In order to avoide accident, you should setting the cups like the picture below：
● The environment have good ventilation, should be 30cm between the wall and other equipments
● Checking the threaded parts such as in the screw, motor shaft and rotational parts,it should use
grease if it is dry
● The chamber should be dry, checking rotors and adapter have admixture or not, do cleaning in
time.
● Continuous use the rotors,rotor is fixed on the motor shaft,rotor nut unscrewing two laps, loose
rotor, then tighten.
● Rotor vertical on the motor shaft, tighten the bolt and rotor.
● Pay attention to the max speed of glass tube, it will have breakage risk when the speeds reach to
more than 4000 RPM.
● When the sample density is more than 1.2 g/m, the max speed must be reduced accordingly.

rotor

cup hanging scroll

cup

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 1. Safety Caution
1.1 Attention in using
1.1.1 Before connecting power
1.1.1.1 The voltage specification of power supply must be same as the input voltage, it should be
single-phase 220V 50Hz to ensure safety ground.
1.1.1.2 In transportation, the rotor head must be removed from the inner barrel and packed alone.
1.1.1.3 Before start, you should checking cracks and corrosion in all rotors and tubes, if find, must be
replaced.
1.1.1.4 You should use the rotors which shipped along with the instruments, when setting the rotor
system, the instruments must be in power off.
1.1.1.5 Keep the chamber cleaning, prevent impurities drop in, if find ,must be ruled out.
1.1.2 In the processing
1.1.2.1 Equipped with magnetic rotor identification system preventsthe rotor from overspeeding
1.1.2.2 After parameter setting,Key””enter for confirmation
1.1.2.3 In “ADD” and “DEC” process, it is normal when you find a short vibration, there is no
necessary to off the power or key “stop”.
1.1.2.4 If loses power in process or forced off the power switch, please not open the door, you should
wait the motor stop(it need about 5~10 mins)
1.1.2.5 When the processing stopped, remember open the door and take the samples out, if you not
open the door, the next centrifugal is not allowed.
1.1.3 After using
1.1.3.1 Please check if there is water in the chamber after running, if you find water, you can remove
the rotor and wipe with soft cloth, keep the chamber cleaning.
1.1.3.2 Please protect the instruments, especially the rotors and tube in order to prevent the polluted
by acid and alkali liquid,you can use soft cloth with neutral cleaning fluid wipe the rotors and
chamber to keep they are dry.
1.1.4 The instruments should stop when it continuous works 60 mins in one time.

1.2 Attention in operating environment

To keep the instruments works stabilit, you should meet the following conditions：
1.2.1 Environment temperature：5℃～40℃
1.2.2 Relative humidity：≤80%
1.2.3 Power voltage：195V～245V，50Hz/60Hz±1Hz
1.2.4 The environment should have good ventilation to keep the chamber no dust, floc, metal scras.
1.2.5 Prevent Corrosive gases and strong electromagnetic interference.
1.2.6 You should keep the instruments works on a solid workbench.

1.3 Transport and storage conditions

1.3.1 When the instruments in package, you should do transport according to the terms in contract,
prevent violent collisions, rain and sun.
1.3.2 After it in package, you should store it in the environment which the RH ≤80%, no corrosion
gas and ventilation is good.

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 2. Main performance indices
2.1 The corresponding parameter of available rotors, please kindly reference the

following table.
BKC-TL6III Main technical parameters and configuration
Model BKC-TL6III
Max speed 6000rpm
Max RCF 5037xg
Speed accuracy ±10rpm
Noise ≦62dB
Timer range 1 ~9999min/ continuous / short spin
N01：Level rotor 100mlx4 5000rpm 4500xg
Adapter 100mlx4 50mlx4 15mlx12(▲) 10ml/15ml/5mlx16
N02：Level rotor 100mlx4 4500rpm 3645xg
Adapter 100mlx4 50mlx8 10ml/15ml/5mlx24
N03：Level rotor 100mlx8 4200rpm 3175xg
Adapter 100mlx8 50mlx8 10ml/15ml/5mlx32（●）
NO4：Cellular crane cup 5mlx40 4200rpm 3175xg
NO5;Angle Rotor 10ml/15ml x12 6000rpm 5037xg
NO6：Mold reaction
(MTP)96 x2 4000rpm 2260xg
plate
Power AC195V～245V 50Hz/60Hz 5A
Weight 55Kg
Dimensions【LxWxH】 425mmx510mmx415mm
Note：The tube with ● is Round-bottom ；The tube with ▲ is sharp-bottom

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 3. Working principle
In operating,place the containers(bottles, tubes) which fill with the test solution into the holes in
rotors, rely on the motor drive high speed rotating to generate RCF to separate the test solution, the
RCF value depends on the rotation radius (r) and speed (n)：

RCF=1.118×10 -5 ×n 2 ×r（×g）……………………（1）

n——Speed（r/min）
r——Turning radius（cm）

27.4×(1nR max -1nR min )μ

T= (min) ……………………（2）
n r (σ-ρ)
2 2

T: The time of the particle separation precipitation in the mixture：

ρ——Mixture density（g/cm³）
μ——Mixture viscosity（P）
n——Speed（r/ min）
r——Particle radius（cm）
σ——particle density（g/cm³）
R max ——horizontal distance between the floor of sample and the axis
R min ——horizontal distance between the liquid level and the axis

R min

R max

Pic. 1 Tube state diagram in running

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 4. Structure diagram and system chart
4.1 Structure Diagram
Mainly composed of the body, rotor transmission parts, damping system, control system, etc. Please
reference the structure diagram as following:

1
2

Pic.2 Centrifuge structure diagram

1.Cover component 2.Hinges assy 3.Rotor system 4.Damping system

5.Motor 6.Chassis 7.Foot-pad

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4.2 System Chart

10A

Keyboard Control Panel

Motor
220V drive
50Hz MCU system
control UNB
system
M

HOL

Pic.3 System Chart

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 5. Installation and Using
5.1 Environment requirements
Please reference the chapter 1

5.2 Rotors Assemble System

5.2.1 When the instrument power off, press the button in the right door by right hand, at the
same time, open the cover lip by left hand, and the door is opened.
5.2.2 Moving motor shaft by hand light and flexibl, you can load the rotors if no abnormal
happend.
5.2.3 After loaded the rotors, place the flat mat on, and then tighten nut with the wrench.

5.3 Control system instructions

5.3.1 The control panel
Please kindly reference the following picture:

Prog Time Rotor rpm Add speed RCF Dec

speed

（1）Display panel

1 2 3 PROG TIME rpm

RCF ENTE
4 5 6 0
Rotor R

STAR
7 8 9 JOG sTOP
T
（2）Operate Panel

5.3.2 Operating instructions

When the instrument power on, it display the setting value before it leave factory, if you want to
modify “time”, “speed”, “RCF”, you can operate with the following steps:
5.3.2.1 Time modify
Timing setting : continuous running and running according to the setting time

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1) continuous running ： when the time window display data, press”time setting ”key twice,
the window display ,you can press”Enter”
2) running according to the setting time ： When the “Time”window display the data,
press”time setting ”key one time, it will blink the current time, press number keys setting
the required time and press ”Enter” for confirmation. When the “Time” window
display ,
(means it in continuous running ) ， Press “Time” key, the “Time” window will blink a
number, such as 60, press the number keys again for modifying the required time, press
“Enter” for confirmation.
5.3.2.2 Setting Speed
Press”rpm” key, and then number keys for setting the speed, after press”Enter” for confirmation. If
the speed changed, the relevant RCF also will changed accordingly and displayed in the window.
5.3.2.3 Setting RCF ： press”RCF” key, and then number keys for setting the RCF value, after
press”Enter” for confirmation. If the RCF changed, the relevant speed also will changed accordingly.
NOTICE: The speed and RCF are set interaction,when both are modified, the later one shall be
the right one.
5.3.2.4 Program mode
There are 20 program memory and 15 acceleration and deceleration rates for standard runs, in each
program, you can storage the time, speed, RCF, add speed and decrease speed, it will help the user
setting according to their requirements
If you want to modify the program mode, press “Prog” key, the window will blink, it means that the
mode in current program mode, press number keys for modifying directly untill you get your
required mode,press “Enter” for confirmation is fine.
If you want to do modify on the content in the program mode, press “Prog” twice, the “Time”
window will blink, please reference 5.3.2.1～ 5.3.2.3, you can do setting or modifying on “Time”,
“rpm”, “RCF”. After setting press”Enter” for confirmation. “Add speed” window will blink and
display the current content in this mode, now you can modify by the number keys, after press
“Enter”，the “RCF” begin to blink, press number keys for modify, “Enter” for confirmation,and then
the “DEC speed” blink and display the current content, press number keys for modifying and
“Enter”for confirmation, a new programing is completed and saved.

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The function of each parts please reference the table 2
Table 2 comparable of function key

Name Description
Display relevant data, when a certain state effectively, the
Status correspond
indicator is on
Setting Timing Set time of centrifugation
Setting Speed Set speed of centrifugation
Setting RCF Set RCF of centrifugation and display the limited speed of rotor
Setting Rotor Used for setting the current rotor number
Setting Program Set program of centrifugation
Enter key Used for confirmation after setting parameters
Number Used for changing parameter
Short Span Used for short span in running
START & STOP Press this button, it start or stop running

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 6. Common fault analysis & removal
Table 4 common fault analysis & removal

FAULT ELIMINATION
REASON ANALYSIS REMARKS
PHENOMENON METHODS
Main circuit fuse(10A) was
Change a same fuse
blown
cable connection to the open box ,then reweld
no display or display board being loosening loosend cable
display disorder misoperation of the
Turn off the machine, Operation under
microcontroller
the reopen after normal
Causes working routine in
minutes working procedure
disorder
press the key or
no pressing the key “enter”,
reoperate
after setup Of parameter
the cover closed badly reclosed
Display
weak voltage insufficient
available, but change voltage
leads to no enough velocity
abnormal
no change of sample after open the door then
centrifugalization
previous centrifugalization reopen
Open box, examine key
bad contact on key switch switch,or change in
need
Running speed Control circuit or frequency change circuit noard or
uneadily converter in trouble frequency converter
instal parts in looseness fasten them
change another same
high noise drive motor not available
one
test tube being imbalance make them symmetry
accelerate and de improper setting on add and setting again according
celerate slowly dec speed to the rotor
reposition the tube
sample in cup is imbalance
sample
sewage in cup clean the sewage
unbalance
protection Replace the same
motor shock absorber aging or specification of shock
loose flange absorber or tighten the
flange screws

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 7. Repair and Maintenance
7.1 The cup should be used in its working life 5 years, the cup needs to be changed if over
the due.
7.2 Not hit the rotor with sharp and hard materials,in transportation or installment , damage
must be avoided, prevent the scratching or other damage which will lead the rotor make
cracks in it.
7.3 You should check the rotor regularly to find if it have corrosion pit/groove/tiny cracks in
it, if you find, please not use it again, and you should contact the manufacturer.
7.4 In the case of sample dipping or dropping on the rotor, the rotor must be stopped and
remove them then clean it partly.
7.5 For protecting oxide layer on the rotor in cleaning the rotors, please wet sponge or
cotton cloth with detergent, and then se distilled water to remove detergent or use 70% ethyl
alcohol to do it, then invert it for drying
7.6 Cut off power when the instruments being not in work

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 8. Special tips and disclaimer
◆Procedure operation must be followed strictly ,especially balancing 、 rotor、 adapter
fixed in the exact position 、 screw tightening 、 is there any crack and corrosion on
accessories.

◆Ensure the chamber, rotor, adapter dry and clean, or some accessories will be corroded in
this kind of environment.

◆Not following the above instructions is belong to human error, which will not be in the
scope of machine quality and warranty. once serious accidents like the phenomenon of
high voice、 even bears breaking、rotor flying out of the chamber etc appears , the user will
have to undertake the corresponding grave consequences.

◆The rotor should be used in its working life, the rotor out of date needs to be changed or
delivered to the manufacturer for balancing. If don’t do it like this, any result will be in the
charge of the owner.

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BIOBASE GROUP
2# building, No.9 Gangxing Road, High-tech Zone, Jinan City, Shandong Province,
China
Tel: +86-531-81219803/01
Fax: +86-531-81219804
Inquiry: [email protected]
Complaints: [email protected]
After-sales service: [email protected]; [email protected]
Web: www.biobase.cc/www.meihuatrade.com / www.biobase.com