Enzymes STEM Case

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Sucharita Majumder

07.03.23

Enzymes STEM Case: Claire


1. Describe Claire’s symptoms. Explain how they were related to
metabolism and homeostasis. [2]
Claire’s symptoms are that she is eating normally but is losing an abnormal
amount of weight. This then leads to her next symptom which is her loss in
energy, which leads to her increased appetite and diet. All of her symptoms
are related to metabolism and homeostasis because metabolism are various
chemical reactions to either break down molecules or combine small
molecules to create bigger molecules and homeostasis is when your body can
maintain stable conditions. Therefore if Claire is having issues with losing
excessive amounts of weight that means that there is a problem with her
metabolism, which is directly related to homeostasis because her body isn’t in
stable condition.

2. Before completing the experiments in the Handbook section, what


was your initial hypothesis? [1]
My initial hypothesis was that something was wrong with her metabolism and
her body wasn’t being able to digest her food, causing her to lose so much
weight.

3. What was your final revised hypothesis? [1]


If Claire has low enzyme production, then that would explain why she isn't
being able to metabolize her food. This is because the low amounts of enzymes
will cause her to have a slower chemical reaction, meaning less of her food
will be broken down and digested.

4. How did Claire’s digestion reaction rates tell you that the problem
was in her small intestine, and not in her stomach? [2]
Her digestion reaction rates told us that when the food was in her stomach, it
was being broken down and was at the right pH level. Compared to when it
traveled to her small intestine, the nutrients were not being absorbed causing
a disturbance in homeostasis. Therefore there is no growth in the cell.
Sucharita Majumder
07.03.23

5. Explain how you determined that Claire had low enzyme


production. Use your experimental data to explain how you ruled
out active site mutations and abnormal pH. [2]
I determined that Claire had low enzyme production because I used a process
of elimination when determining what was the problem. When looking at the
experimental data, all the substrates fit into their following amino acid and
the pH aligned with the control group (the healthy dog) which meant that
Claire’s pH levels were normal. That leaves the low production of enzymes,
which was proven correct by the experimental data. The production of
enzymes was extremely low compared to the control group which was shown
by the graph.

6. How does Claire’s diagnosis explain why 1 mL of liquid from her


small intestine digested protein slowly but 1 mg of her pure
protease digested protein normally? [2]
Claire’s diagnosis explains why 1 mL of liquid from her small intestine digested
protein slowly but 1 mg of her pure protease digested protein normally
because protease is an enzyme that aids in protein digestion. Together with
protease, one milliliter of liquid from the small intestine contains other
elements. As a result, the digestion process by these proteases was slower
than that of pure proteases.

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