Rajiv Gandhi University of Health Sciences, Karnataka

4th T Block Jayanagar, Bengaluru

Curriculum delivery design of B. Pharm. course of Semester VII System
w.e.f Academic year 2020-21
SEMESTER-VII
BP701T: INSTRUMENTAL METHODS OF ANALYSIS (Theory)

Scope: This subject deals with the application of instrumental methods in qualitative and
quantitative analysis of drugs. This subject is designed to impart a fundamental knowledge on
the principles and instrumentation of spectroscopic and chromatographic technique. This also
emphasizes on theoretical and practical knowledge on modern analytical instruments that are
used for drug testing..
2. Departmental objectives (what the learners will be able to perform after completing the
subject):
A. Learning Objectives:
Upon completion of this course the student should be able to

i. Understand the interaction of matter with electromagnetic radiations and its applications
in drug analysis
ii. Understand the chromatographic separation and analysis of drugs.
iii. Perform quantitative & qualitative analysis of drugs using various analytical instruments.

3. Annual objectives (for each year, if the subject is spread over different years):NA
4. Content distribution as per the list of topics, time allotted for each topic, distribution for
‘Must know’, ‘Desirable to know’ and ‘Nice to know’ and the probable weightage.
The following table can also be a reference frame for continuous and formative assessment of
learning. If the curriculum management is scheduled as per the tabulation, there can be
clarity for both learners and teachers to take stock of the mastery achieved in each objective.
This will also help for professional excellence that goes beyond the examination process.

UNIT-I Hours: 10 Weightage: 24 Marks

Topics
Learning content
distribution UV Visible spectroscopy and Fluorimetry
UV Visible spectroscopy: Introduction, Nature of EMR, Energies
Must to know associated with the organic molecules, Electronic transitions,
Chromophores, Auxochromes, Beer and Lambert’s law, Derivation and
 Deviations. Instrumentation - Sources of radiation, wavelength
selectors, sample cells, detectors- Barrier layer cell, Photomultiplier
tube, Photo voltaic cell, Spectrophotometric titrations.
Fluorimetry: Introduction, Theory, Jablonski process, concepts of
singlet, doublet and triplet electronic states, internal and inter system
crossing, inner filter effect, factors affecting fluorescence, quenching,
instrumentation and applications.
Energy of EMR, wavelength, frequency, wave number, absorbance,
Transmittance, absorptivity, molar extinction co-efficient, Color wheel,
Desirable to know Solvent effect on absorption spectra, spectral shifts, Photo tube, Silicon
Photodiode, Single component and multi component analysis.
Equilibrium constant and rate constant.
K bands and R bands, E band and B band, forbidden and allowed
Nice to know
transitions, Woodward Fieser rule.

UNIT-II Hours: 10 Weightage: 21 Marks

Topics
Learning content
distribution IR spectroscopy, Flame Photometry, Atomic absorption spectroscopy,
Nephloturbidometry
IR Spectroscopy: Introduction, criteria of a molecule to absorb IR,
modes of vibrations in molecules, sample handling, Instrumentation-
Sources of radiation, wavelength selectors, detectors –Bolometer, Golay
cell, Thermocouple Thermistor, Pyroelectric detector and applications of
IR.
Atomic Spectroscopy:
Must to know
Flame Photometry: Introduction, Principle, Events occurring in the
flame, structure of flame, instrumentation and applications of flame
photometry.
Atomic Absorption spectroscopy: Principle, thermal atomizers and
applications.Interferences in Atomic spectroscopy.
Nepheloturbidometry Principle, instrumentation and applications
Vibrational frequency of alcholol, aldehyde, ketone, carboxyl, amine,
Desirable to know
amide.
Hooke’s law in IR spectroscopy, FTIR, NIR, fuel and oxidants used in
Nice to know flame emission spectroscopy, differentiates fluorimeter and
Nephelometer, Colorimeter and turbidimeter.

UNIT-III Hours: 10 Weightage: 19 Marks

Learning content Topics
distribution
Introduction to chromatography, Column chromatography, TLC, PC
 and Electrophoresis
Chromatographic principle and its classifications.
Column chromatography: Introduction, Methodology, advantages,
disadvantages and applications.
Thin layer chromatography: Introduction, preparation, activation and
Must to know visualization, advantages, disadvantages and applications,
Paper chromatography: Introduction, development techniques,
visualization, advantages, disadvantages and applications.
Electrophoresis– Introduction, factors affecting electrophoresis mobility,
Techniques of paper, gel and applications.
Isocratic and gradient, Normal Phase and Reverse Phase Chromatography,
analytical and preparative, Frontal, displacement and elution analysis, Rf,
Desirable to know Rx and Rm values, Classification and ideal properties of adsorbents,
detecting reagents, Silica Gel GF254, edge effect, two-dimensional
chromatography, capillary electrophoresis.
Eluotropic series of solvents, Difference between TLC and HPTLC,
Nice to know Stahl’s triangle in TLC, Moving boundary electrophoresis, isoelectric
focusing electrophoresis.

UNIT-IV Hours: 08 Weightage: 17 Marks

Topics
Learning content
distribution Gas Chromatography and High-Performance Liquid
Chromatography
Theories of Chromatography: Plate theory and Rate theory
Gas Chromatography: Introduction, types, instrumentation, advantages,
disadvantages and applications.
Must to know
High-Performance Liquid Chromatography: Introduction, types,
instrumentation, advantages and applications.
Stationary phases of GC & HPLC.
Temperature programming in GC, derivatization in GC, Guard column,
Desirable to know
system suitability factors.
Nice to know LC-MS and GC-MS.
UNIT-V Hours: 07 Weightage: 14 Marks
Topics
Learning content
distribution Ion exchange chromatography, Gel chromatography and Affinity
chromatography
Must to know Ion exchange chromatography: Introduction, mechanism, classification
of ion exchange resins, factors affecting ion exchange and applications.
Gel chromatography: Introduction, principle, various gels used,
instrumentation and applications.
Affinity chromatography: Introduction, principle, various ligands used
 and applications.
Properties of ion exchange resins, regeneration of cation and anion
Desirable to know exchange resin, theory of gel chromatography, theory of affinity
chromatography.
Ion exchange capacity, Size exclusion chromatography, Chiral
Nice to know
chromatography, Ion Chromatography.

5. Blueprint of question paper, for each QP. This shows the weightage given to each chapter
in the summative assessment. This improves the content validity by distributing the
assessment of learners in the competencies that are represented by learning objectives
under each chapter.
State the number of QPs for the subject.
The following template demonstrates how each QP Blueprint would look like:

BLUE PRINT OF MODEL QUESTION PAPER

BP701T: Instrumental Methods of Analysis (Theory)
TIME: 3 HOURS MAX. MARKS: 75
Must know Desirable to know
Unit LE Weightage
Hours SE SA LE SE SA
No (10X3 of marks
(5X8) (2X5) (10X0) (5X1) (2X5)
)
Unit-I 10 1 1 1 - 1 1 24
Unit-II 10 1 1 2 - - 1 21
Unit-III 10 - 3 - - - 2 19
Unit-IV 08 1 1 - - - 1 17
Unit-V 07 - 2 2 - - - 14
Total 45 30 40 10 - 5 10 95
80 15 95
* 80 % of the questions shall be from the Must Know area and 20 % shall be from the
Desirable to Know area of the Curriculum.