NIHON KOHDEN reagents

MEK 7222K MEK 6410K

REF REF
8-522 DILUENT NK (20 L) 8-522 DILUENT NK (20 L)
8-523 LYSING REAGENT NK 3 (1 L) 8-523 LYSING REAGENT NK 3 (1 L)
8-524 LYSING REAGENT NK 5 (1 L) 8-525 CLEANING REAGENT NK (5 L)
8-525 CLEANING REAGENT NK (5 L) 8-527 ENZYMATIC CLEANER NK (1 L)
8-527 ENZYMATIC CLEANER NK (1 L)
IVD

SUMMARY COMPONENTS
The blood counters use the impedance technology to measure the DILUENT NK LYSING REAGENT NK 3
number of cells in a diluted blood sample which pass through an sodium chloride < 5 g/l oxalates < 25 g/l
aperture located between two electrodes where a constant electrical organic buffer < 2 g/l dodecyltrimethyl-
current is applied. sodium sulphate < 10 g/l ammoniumchloride 3.1-4.0 g/l
The dilution is done with an isotonic solution which is a conductor and Na2 EDTA < 0.5 g/l bretol < 2 g/l
does not lyse the blood cells. Preservative < 0.05 g/l
The conductivity of the isotonic diluent allows the passage of the LYSING REAGENT NK 5 CLEANING REAGENT NK
electrical current between the two electrodes. When a particle is sodium dodecyl sodium hypochlorite 14 g/l
aspirated through the micro-orifice, it moves its own volume of sulphate < 1.5 g/l
electrolyte. This applies a modification of the resistance between both inorganic buffer < 2.5 g/l
electrodes and generates an electrical pulse. The amplitude is directly sodium chloride < 9 g/l
proportional to the volume of the particle. preservative < 1 g/l
Two separate dilutions are prepared for WBC/HGB and for RBC/PLT. sodium hydroxide 1M < 6 g/l
ENZYMATIC CLEANER NK
COLLECTION AND STORAGE
boric acid < 5 g/l
NIHON KOHDEN MEK 7222K, MEK 6410K are fully automated sodium tetraborate decahydrate < 0.8 g/l
analysers performing haematological analysis on whole blood collected non-ionic surfactant < 2 g/l
on EDTA tubes. The ratio between EDTA and whole blood must be sodium chloride < 5 g/l
between 1 to 2 mg per ml of blood. preservative < 0.05 g/l
The samples should be used at room temperature no longer that 4 enzymes < 13 g/l
hours after collection. If the analysis can’t be done in the time, the WASTE TREATMENT
samples should be stored at 4°C. Chemical residues, in general, are included into special waste.
Disposing of the latter is regulated by appropriate laws and
UTILISATION ordinances. We recommend contacting the appropriate authorities, or
waste disposal enterprises that will advise you on how to dispose
Before running the analysis, the sample should be gently mixed. special waste of.
Open the tube, place it in the sampling port and press the start key. PRECAUTIONS
For In vitro diagnostic use.
CONSERVATION AND SHELF LIFE For professional use only.
CLEANING REAGENT NK meeting the criteria for classification in
Diluent NK, Lysing Reagent NK 3, Lysing Reagent NK 5, Enzymatic accordance with Regulation (EC) No 1272/2008.
Cleaner NK must be stored between 18°C and 30°C and used before the Ingredients:
expiry date indicated on the label. CLEANING REAGENT NK contains sodium hypochlorite
Warning.
Cleaning Reagent NK must be stored between 2°C and 25°C and used H315 Causes skin irritation.
before the expiry date indicated on the label. H319 Causes serious eye irritation.
P280 Wear protective gloves/protective clothing/eye
REFERENCE protection/face protection.
P302+P352 IF ON SKIN: Wash with plenty of soap
Refer to the Operator manual for the analysers. and water.
P305+P351+P338 IF IN EYES: Rinse cautiously with water for
UTILISATION (For In Vitro Diagnostic use) several minutes. Remove contact lenses, if present and easy to do.
Continue rinsing.
DILUENT NK is designed for diluting the whole blood prior to counting
LYSING REAGENT NK 3 meeting the criteria for classification
and sizing of RBC/WBC/PLT. It maintains stability RBC/PLT during in accordance with Regulation (EC) No 1272/2008.
counting. Ingredients:
LYSING REAGENT NK 3 / NK 5: Lysing agent to obtain the LYSING REAGENT NK 3 contains dodecyltrimethylammonium
chloride.
measurement of the haemoglobin, counting and differentiation of the white Danger.
blood cells. H400 Very toxic to aquatic life.
Use in combination with the diluent, this reagent lyses the red blood cells P273 Avoid release to the environment.
and protects the state of the leukocytes to permit the differentiation in P391 Collect spillage.
three populations (lymphocytes, monocytes, granulocytes). P501 Dispose of contents/container in accordance with
Diluent, lysing reagent and cleaning reagent are the functional set to local regulation.
perform blood sample analysis on haematology analyser. For further information please refer to Material Safety Data Sheet.
CLEANING REAGENT NK is designed to remove protein contaminants
from the measurement system of the analyser after each blood sample
analysis.
ENZYMATIC CLEANER NK NAME AND ADDRESS OF THE MANUFACTURER
is designed to remove protein contaminants from the measurement
system of the analyser after each blood sample analysis. The presence PZ CORMAY S.A.
of an enzyme reduces the formation of proteins deposit. 22 Wiosenna Street
05-092 Łomianki, Poland
tel.: +48 (0) 22 751 79 10
fax: +48 (0) 22 751 79 14
http://www.cormay.pl

06/17/06/17 Page 1 of 1