Scribd Logo
Upload

Welcome to Scribd!

  • 13 views

    Wayback Machine

    Uploaded by

    nataliapiuco

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    Wayback Machine

    Uploaded by

    nataliapiuco
    13 views1 page

    Copyright

    © © All Rights Reserved

    Available Formats

    PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    13 views1 page

    Wayback Machine

    Uploaded by

    nataliapiuco

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 1

    Go FEB MAR DEC

    http://www.ccsenet.org/journal/index.php/jas/article/download/0/0/38724/39
    ! ⍰❎
    3 captures 19 f%
    19 Mar 2020 - 16 Jan 2022 2019 2020 2021 ▾ About this capture

    Journal of Agricultura
    ISSN 1916
    Published by Canadian Ce

    Temporary Immersion Bioreactors: Establishmen


    Diego Pascoal Golle1, Jana Koefender1, Roberta Cattaneo Horn2, Natá
    Cristiane Kaiper1 & Juliane Nicolodi Camera1
    1
    Alto Jacui Technological Innovation Center, University of Cruz Alta, Cruz Alta, Bra
    2
    Oxidative Stress Laboratory, University of Cruz Alta, Cruz Alta, Brazil
    Correspondence: Diego Pascoal Golle, Laboratory of Plant Tissue Culture, Alto Jacu
    Center, University of Cruz Alta, Rodovia Municipal Jacob Della Méa, km 5.6, Parad
    Alta, RS, Brazil. Tel: 55-553-321-1606. E-mail: [email protected]

    Received: December 10, 2018 Accepted: January 15, 2019 Online Publi
    doi:10.5539/jas.v11n4p176 URL: https://doi.org/10.5539/jas.v11n4p176

    This research is financed by the World Bank and by the Program of Technological
    Economic Development, Science and Technology of Rio Grande do Sul State, Brazil.

    Abstract
    Cassava (Manihot esculenta Crantz-Euphorbiaceae) is one of the most important crop
    the main source of many family’s calorie intake and contributes toward food
    necessitated sterile vegetative propagules with essential physiological and genetic qua
    by plant tissue culture techniques. However, the maintenance of in vitro propa
    automation, making large-scale production feasible. This work aimed at deve
    establishment of cassava cultivation in Temporary Immersion Bioreactors (TIBs).
    medium reduced to half-strength of the salt concentration and the same form
    6-Benzylaminopurine [BAP]) and different culture cycles were evaluated in B
    immersion and 4 hours of stationary phase [15M4H]; 15 minutes of immersion and
    [15M8H]). The establishment of culture in bioreactors was promising. Based on o
    medium without growth regulators was more effective for shoot formation; there were
    cycle when growth regulators were used. However, the 15M8H cycle had better perfo
    with or without growth regulators. The use of culture medium without BAP led to be
    increased when BAP was used. The present research investigation indicated the use
    half- strength the salt concentration without the presence of growth regulator and th
    constituted by 15 minutes immersion and 8-hour intervals in stationary phase w
    cultivation in TIBs.
    Keywords: bioreactors, tissue culture, automation, Manihot esculenta
    1. Introduction
    Cassava (Manihot esculenta Crantz-Euphorbiaceae) is a very important crop in seve
    regions of the world, especially in countries of Africa, Latin America and the Cari
    Cassava is considered as the main source of calories for many populations in develop
    favor the comprehensive geographic distribution of cassava, with emphasis on its gro
    areas, which indicated the potential of this plant to be grown in regions affected
    benefits of cassava are its use for animal feed, starch production, food processing,

    You might also like