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    Genetics-2 Course Outline

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    shazeen shoaib
    This course provides information on molecular markers, linkage mapping, genome structure, gene function, regulation, and crop evolution. Topics include recombinant DNA techniques, applications, genetic change mechanisms, human and plant genome projects, and bioinformatics. Practicals involve DNA and protein gel electrophoresis and PCR. Recommended books cover biotech, applied microbiology, plant biology, gene technology, genetics, and plant genetic diversity.

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    Genetics-2 Course Outline

    Uploaded by

    shazeen shoaib
    45 views2 pages
    This course provides information on molecular markers, linkage mapping, genome structure, gene function, regulation, and crop evolution. Topics include recombinant DNA techniques, applications, genetic change mechanisms, human and plant genome projects, and bioinformatics. Practicals involve DNA and protein gel electrophoresis and PCR. Recommended books cover biotech, applied microbiology, plant biology, gene technology, genetics, and plant genetic diversity.

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    This course provides information on molecular markers, linkage mapping, genome structure, gene function, regulation, and crop evolution. Topics include recombinant DNA techniques, applications, genetic change mechanisms, human and plant genome projects, and bioinformatics. Practicals involve DNA and protein gel electrophoresis and PCR. Recommended books cover biotech, applied microbiology, plant biology, gene technology, genetics, and plant genetic diversity.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    45 views2 pages

    Genetics-2 Course Outline

    Uploaded by

    shazeen shoaib
    This course provides information on molecular markers, linkage mapping, genome structure, gene function, regulation, and crop evolution. Topics include recombinant DNA techniques, applications, genetic change mechanisms, human and plant genome projects, and bioinformatics. Practicals involve DNA and protein gel electrophoresis and PCR. Recommended books cover biotech, applied microbiology, plant biology, gene technology, genetics, and plant genetic diversity.

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    © All Rights Reserved
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    Semester: VIII

    Course Code: BOT-407/407L


    Course Title: Genetics-II
    Credit Hours: 3 (2+1)
    Course Outcome:
    This course will provide basic information about molecular markers and linkage mapping,
    prokaryotic and eukaryotic genome-structure, gene function and regulation, epigenetics,
    cytogenetics and crop evolution.
    Course Outline:
    Recombinant DNA: Recombinant DNA Technology Introduction, basic techniques, PCR and Rt
    PCR, Restriction enzymes, Plasmids, Bacteriophages as tools, the formation of recombinant
    DNA, recombinant DNA technology, site directed mutagenesis, DNA sequencing.
    Application of Recombinant DNA: Applications of recombinant DNA technology using
    prokaryotes, recombinant DNA technology in eukaryotes: An overview, transgenic yeast,
    transgenic plants, transgenic animals, screening for genetic diseases, identifying disease genes,
    DNA typing, gene therapy, genetically modified organisms and apprehensions.
    Mechanisms of Genetic Change I: Gene Mutation: The molecular basis of gene mutations,
    spontaneous mutations, induced mutations, reversion analysis mutagens and carcinogens,
    biological repair mechanisms.
    Mechanisms of Genetic Change II: Recombination: General homologous recombination, the
    Holiday model, enzymatic mechanism of recombination, site-specific recombination,
    recombination and chromosomal rearrangements.
    Mechanisms of Genetic Change III: Transposable Genetic Elements: Insertion sequences,
    transposons, rearrangements mediated by transposable elements, review of transposable elements
    in prokaryotes, controlling elements in maize.
    Human Genome Project: Strategies and application, achievement and future prospects.
    Plant Genome Projects: Arabidopsis, achievement and future prospects. Other plant genome
    projects
    Bioinformatics: Application of computational tests to the analysis of genome and their gene
    products
    Bioethics: Moral, religious and ethical concerns
    Practical Outline:
    1. Problems relating to the theory.
    2. Isolation and separation of DNA and protein on gel electrophoresis: Bacterial chromosome,
    Plasmid DNA (mini-preps), Plant DNA, Protein.
    3. DNA Amplification by PCR.

    Recommended Books:
    1. Stevens, T. & Newman, S., (2019). Biotech juggernaut: hope, hype, and hidden agendas of
    entrepreneurial bioscience (1st ed.). Philadelphia: Routledge.
    2. Shukla, P., (2018). Applied microbiology and engineering: an interdisciplinary approach (1st
    ed.). London: Academic Press.
    3. Venkat, B., Sahijramand, R. & Murthy, K., (2015). Plant biology and biotechnology (2nd
    ed.). New York: Springer Publications.
    4. Winnacker, E. L.2003, From Gene to Clones Introduction to Gene Technology, Panima
    Publishing Corporation, New Delhi.
    5. Miglani, 2003, Advanced Genetics, Narosa Publishing House, India,.
    6. Hartt, D. L, and Jones, E. W. 2005. Genetics, Analysis of Gene and Genomes. Jones and
    Bartlett Publishers, Sudbury, USA
    7. Primrose, S. B., Twyman, R. M. and Old R. W. 2004. Principles of Gene Manipulation, an
    Introduction to Genetic Engineering (6th Edition), Blackwell Scientific Publications.
    8. Mahmut Caliskan. 2012. The Molecular basis of plant genetic diversity. In Tech Publishers.
    9. David Hyde. 2008. Introduction to Genetic principles. McGraw-Hill.

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