Programme/Class: Diploma Year: Second Semester: Fourth

Subject: Z00LOGY

Course Code: BO50401T Course Title: Gene Technology and Human Welfare
Course outcomes:
The student at the completion of the course will be able to:
.Understand the principles of genetic engineering, how genes can be cloned in bacteria and the
various technologies involved in it.
Know the applications of biotechnology in various fields like agriculture, industry and human health.
Know the basics of industrial biotechnology.
Get introduced to DNA testing and utility of genetic engineering in forensic sciences.
. Get introduced to
computers and use of bioinformatics tools.
Enable students to get employment in pathology/Hospital.
Take up research in biological sciences.
Credits: 4 Core: Compulsory

Max. Marks: 25+75 Min. Passing Marks: as per rules

Total No. of Lectures-Tutorials-Practical (in hours per week): L-T-P: 4-0-0

Unit Topic Total No. of

Lectures (60)
Principles of Gene Manipulation 10
Recombinant DNA Technology
Restriction Enzymes, DNA modifying enzymes, Cloning
Vectors, Ligation
Gene transfer techniques, Gene therapy
Selection and identification ofrecombinant cells
Applications of Genetic Engineering
Single cell proteins
Biosensors, Biochips
Crop and live stock improvement, development of transge
Development of DNA drugs and
vaccines
Enzyme Technology
Microbial culture
Methods of enzyme production
Immobilization of enzymes
Applications, antibiotics
IV DNA Diagnostics 6
Genetic analysis of human diseases, detection of knmown
and unknown mutations
DNA fingerprinting
Concept of pharmacogenomics and pharmacogenetics
Personalized medicine-optimizing drug therapy
V Biostatistics
3
. Calculations of mean, median, mode, variance, standard
deviation
. Concepts of coefficient of variation, Skewness, Kurtosis

22
 V
Elementaryidea of probability and application
Biostatistics |1
Data summarizing:
frequency distribution,
presentation-bar, pie diagram, histogram graphical
Tests of
significance: one and two sample tests, t-test
and Chi-square test
VIl Basics of Computers
Basics (CPU, 1/O units) and
operating systems
Concept of homepages and websites, World Wide Web,
URLS, using search engines

VIll Bioinformatics
Databases: nucleic acids, genomes,
and structures, Bibliography
protein sequences
Sequence analysis (homology): pairwise and multiple
sequence alignments-BLAST, CLUSTALW
Phylogenetic analysis
Suggested Readings:

1. Primrose & Twyman. Principles of Genome Analysis and Genomics. Blackwell (2003).
2. Hartl & Jones. Genetics: principles & Analsysis of Genes & Genomes. Jones & Bartlett (1998).
3. Sambrook et al .Molecular Cloning Vols 1, 1, I1.CSHL (2001).
4. Primrose. Molecular Biotechnology. Panima (2001).
5. Clark&Switzer. Experimental Biochemistry. Freeman (2000)
Sudbery. Human Molecular Genetics. Prentice-Hal (2002).
7 Wilson. Clinical Genetics-A Short Course, Wiley (2000).
8. Pasternak. An Introduction to Molecular Human Genetics. Fritzgerald (2000).
9 Biostatistical Analysis (Fourth Edition) by Jerrold H. Zarr, Pearson Education Inc., Delhi.
10. Statistical Methods (Eighth Edition) by G. W. Snecdecor and W. G. Cochran, Willey Blackwell
11. Biostatistics (Tenth Edition) by W.w. Daniel and C. L Cross, Wiley
12. Introductory Biological Statistics (Fourth Edition) by John E. Havel, Raymond E. Hampton and Scott .
Meiners
13. Westhead et al Bioinformatics: Instant Notes. Viva Books (2003).

Course Books published in Hindi must be prescribed by the Universities and Colleges

This course can be opted as an elective by the students of following subjects:

The eligibility for this paper is 10+2 with Biology as one of the subject

Suggested Continuous Evaluation Methods:

House Examination/Test: 10 Marks

Written Assignment/Presentation/Project/Research Orientation /Term Papers/Seminar: 10 Marks

Class performance/Participation: 5 Marks

Further Suggestions: None

At the End of the whole syllabus any remarks/ suggestions

23
Programme/Class: Degree Year: Second Semester: Fourth
Subject: Z0OLOGY

Course Code: BO50402P/R Course Titte: Genetic

Engineering Lab, Genetic Counselling
&Telemedicine
Course outcomes:
The student at the completion of the course will be able to:
Understand the principles of genetic engineering with hands-on experiments in mutation
testing of infectious diseases like Covid 19. detection,
Get introduced to DNA testing and utility of genetic engineering in forensic sciences.
Apply knowiedge and awareness of the basic principles and concepts of biology. computer science
and mathematics existing software effectively to extract information from large databases and to use
this information in computer modeling.
Use bioinformatics toots to find out evolutionary/phylogenetic relationship of oganisms uslng gene
sequences.
Get employment in Hospitals/Diagnostic and forensic labs/Counsel families with genetic disorders.
Enable students to take up research in biological sciences.
Credits: 2 Core: Compulsory

Max. Marks: 25+755 Min. Passing Marks: as per rules

Total No. of Lectures-Tutorials-Practical (in hours per week): L-T-P: 0-0-4

Unit Topic Total No. of

Lectures (60)
1. Measure the pre and post clitellar lengths of earthworms 10
and calculate mean, median, mode, standard deviation etc.
2. Measure the height and weight ofall students in the class
and apply statistical measures.

1. To perform bacterial culture and calculate generation 20

time of bacteria.
2. To study Restriction enzyme digestion using teaching
kits
3. To study Polymerase Chain Reaction (PCR) using
teaching kits.
4. Demonstration of agarose gel electrophoresis for
detection of DNA.
Demonstration of Polyacrylamide Gel Electrophoresis
(PAGE) for detection of proteins. DNA and
of unknown
6. To calculate molecular weight
protein fragments from gel pictures.

applications 15
1. To learn the basics of computer
BLAST
2. To learn sequence analysis using
alignment using
3. To learn Multiple sequence
CLUSTALw
analysis using the
4. To learn about Phylogenetic
programme PHYLIP.
Primer designing for PCR
5. Tolearn how to perform
24
 using avalable softwares etc.
IV Virtual Labs
15
1. Gel Documentation System-
2. https://youtu.be/WPpt3-FanNE
Colorimeter- htps://youtu.be/v4ak6G0bGuU
3. PCR Part 1-
4. PCR Part 2- https://youtu.be/CpGX1UFSI4A
5. https://voutu.be/6lcHAYPTAEW
DNA isolation Part 1

6.
https://youtu.be/QE7UIOJnY9A
DNA solation part https://youtu.be/-
2-
efr HFeHxM
7. DNA curve-
8. https://youtu.be/ubL8QXTeuG4
Spectrophotometer

9.
https://youtu.be/ubL8QXTeuG4
Agarose Part 1- https://youtu.be/7gvHPFww--g
10. Agarose part 2- https://youtu.be/j bozCHNSSg
Suggested Readings:
1. Primrose & Twyman. Principles of Genome
2. Hartl & Jones. Genetics: principles &
Analysis and Genomics. Blackwell (2003).
3.
Analsysis of Genes & Genomes. Jones & Bartlett (1998).
Sambrook et al .Molecular Cloning Vols l, I), 11. CSHL
4.
(2001).
Primrose. Molecular Biotechnology. Panima
(2001).
Course Books published in Hindi must be prescribed by the Universities and Colleges
This course can be opted as an elective by the students of following subjects:
The eligibility for this paper is 10+2 from Arts/Commerce/Science
Suggested Continuous Evaluation Methods:
House Examinatlon/Test: 10 Marks
Written Assignment/Presentation/Project /Term Papers/Seminar: 10 Marks
Class performance/Participation: 5 Marks

Further Suggestions: None

At the End of the whole syllabus any remarks/ suggestions: