Academic Council Date –

Item No. –

Janardan Bhagat Shikshan Prasarak Sanstha’s

CHANGU KANA THAKUR

Arts, Commerce and Science College, New Panvel (Autonomous)
Re-accredited A+ Grade by NAAC (Third Cycle-CGPA-3.61)
‘College with Potential for Excellence’ Status Awarded by UGC
‘Best College Award’ by University of Mumbai

As per National Education Policy - 2020

Title of the Programme
M.Sc.in Microbiology
(Faculty of Science)

Syllabus for M. Sc. (Microbiology)

Semester III

(With effect from the academic year 2024-25)

Changu Kana Thakur Arts, Commerce and Science College, New Panvel (Autonomous)
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 Janardan Bhagat Shikshan Prasarak Sanstha’s

CHANGU KANA THAKUR

Arts, Commerce and Science College, New Panvel (Autonomous)

As per National Education Policy - 2020

Sr. No. Heading Particulars
1 Title of program M. Sc. in Microbiology
Students must have earned mandatory credits of
2 Eligibility
Microbiology.
3 Duration of program 02 Years for Post Graduate Degree
4 Intake Capacity 12
Theory 100 Marks; Internal: External 40:60;
5 Scheme of Examination
Practical 50 Marks
6 Standards of Passing 40%
7 Semesters III
Program Academic 5.0
8
Level
9 Pattern Revised as per NEP 2020
10 Status Approved by BOS and Academic Council
To be implemented from Academic Year 2024-25
11
Academic Year

Signature of Signature of

Name Prof. (Dr.) S.K. Patil

Head, Department of __________ Principal
Changu Kana Thakur Changu Kana Thakur
A.C.S. College, New Panvel A.C.S. College, New Panvel
(Autonomous) (Autonomous)

Changu Kana Thakur Arts, Commerce and Science College, New Panvel (Autonomous)
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 Janardan Bhagat Shikshan Prasarak Sanstha’s

CHANGU KANA THAKUR

Arts, Commerce and Science College, New Panvel (Autonomous)

Preamble
1) Introduction
With the introduction of the National Education Policy-2020, Changu Kana Thakur
Arts, Commerce, and Science College, New Panvel, have restructured and revised the
existing syllabus of M.Sc.-II Microbiology for the academic year 2023-2024. The
revised syllabus, aligned with the CBCS pattern, will be implemented from 2024-2025,
aiming to foster a more knowledge-oriented approach in Microbiology.
The new syllabus embraces an interdisciplinary perspective, integrating concepts from
Biochemistry, Bacterial Taxonomy, and Molecular Biology, while ensuring it remains
accessible and manageable for M.Sc. students. It is meticulously designed to provide
both foundational and advanced knowledge progressively from the first year to the third
year.
This revamped curriculum not only caters to the evolving needs of students but also
aims to inspire them to pursue further studies in Microbiology or explore
entrepreneurial opportunities. Moreover, it equips students with the skills necessary for
employment in Microbiology-based industries

2) Aims and Objectives

 To align the M.Sc.-II Microbiology syllabus with the National Education Policy-2020
and the CBCS pattern.
 To foster a more knowledge-oriented approach in Microbiology education.
 To integrate interdisciplinary concepts from Biochemistry, Bacterial Taxonomy, and
Molecular Biology into the syllabus.
 To ensure accessibility and manageability for M.Sc. students while maintaining
academic rigor.
 To provide a curriculum that progresses from foundational to advanced knowledge
over the course of the program.

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3) Learning Outcomes
 Understand the structural organization and function of intracellular organelles.
 Analyse the principles of membrane transport and intracellular compartmentalization
 Interpret the mechanism of cell division, cell cycle regulation, programmed cell death
(apoptosis), and cell-cell adhesion.
 Apply the acquired knowledge and skills in conducting experiments, analyzing data,
and drawing conclusions in the field of Cell Biology.
 Discuss the principles of cell communication, including signaling pathways through
G-protein linked receptors, enzyme-linked receptors, and signaling in plants.

4) Credit Structure of the M.Sc. (Microbiology) Semester III and IV

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 Abbreviations Used

 POs : Program Outcomes

 PS : Program Structure
 PSOs : Program Specific Outcomes
 COs : Course Outcomes
 TLP : Teaching-Learning Process
 AM : Assessment Method
 DSC : Discipline Specific Core
 DSE : Discipline Specific Elective
 GE : Generic Elective
 OE : Open Elective
 VSC : Vocational Skill Course
 SEC : Skill Enhancement Course
 IKS : Indian Knowledge System
 AEC : Ability Enhancement Course
 VEC : Value Education Course
 OJT : On Job Training (Internship)
 FP : Field project
 CEP : Community engagement and service
 CC : Co-curricular Courses
 RM : Research Methodology
 RP : Research Project
 MJ : Major Course
 MN : Minor Course

Changu Kana Thakur Arts, Commerce and Science College, New Panvel (Autonomous)
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 Janardan Bhagat Shikshan Prasarak Sanstha’s

CHANGU KANA THAKUR

Arts, Commerce and Science College, New Panvel (Autonomous)

Program Outcomes (POs)

POs Statement
PO Knowledge and
No. After completing the Bachelor of Science Program, Skill
students will be able to-
PO-1
PO-2
PO-3
PO-4
PO-5

Changu Kana Thakur Arts, Commerce and Science College, New Panvel (Autonomous)
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 Janardan Bhagat Shikshan Prasarak Sanstha’s

CHANGU KANA THAKUR

Arts, Commerce and Science College, New Panvel (Autonomous)

Program Specific Outcomes (PSOs)

PSOs Statement
PSO Knowledge and
No. After completing the Bachelor of Science Program, Skill
students will be able to-
PSO-1
PSO-2
PSO-3
PSO-4
PSO-5

Changu Kana Thakur Arts, Commerce and Science College, New Panvel (Autonomous)
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 Janardan Bhagat Shikshan Prasarak Sanstha’s

CHANGU KANA THAKUR

Arts, Commerce and Science College, New Panvel (Autonomous)

Syllabus for M.Sc. (Microbiology) Semester III

Choice Based Credit System
Under New Education Policy (NEP) 2020
(To be implemented from the academic year 2024-2025)

Course Code : PSC3MI-3

Course Title : Cell Biology

Course Type: Major No. of Credits: 03

Course Outcomes (Cos)

CO COs Statement
No. After completing the Bachelor of Science Program, students will be able to-
CO-1 Understand the Cell Structure and Function.
CO-2 Explore the Cellular Processes and Regulation in eukaryotic cell.
CO-3 Analyse the Cell Communication, Signalling pathways and its role in
multicellular development of organism.
CO-4 Understand the fundamentals process of cell cycle and its regulation.
CO-5 Understand the mode of cell communication in multicellular organism.
CO-6 Understand the fundamental concepts of cell biology.

Changu Kana Thakur Arts, Commerce and Science College, New Panvel (Autonomous)
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 Syllabus for M.Sc. (Microbiology) Semester III
Choice Based Credit System
Under New Education Policy (NEP) 2020

Course Code : PSC3MI-3

Course Title : Cell Biology

Unit I : Introduction to Cell Biology and Cell Membrane

Unit II : Organization and Function of Mitochondria, Chloroplast and Cytoskeleton

Unit III : Cell Division, Cell Cycle and Cell Junctions

Unit IV : Cell Communication and Multicellular Cell Development

Unit/Subunit
Topics Lectures

Unit-1 Introduction to Cell Biology and Cell Membrane 15

1.1 Introduction to cell biology: Structural organization and function of 02
intracellular organelles (Cell wall, nucleus, mitochondria, Golgi
bodies, lysosomes, endoplasmic reticulum, peroxisomes, plastids,
vacuoles, chloroplast
1.2 Membrane structure: Cell membrane structure: Lipid bilayer, 03
membrane proteins, Spectrins, Glycophorin, Multipass membrane
proteins Bacteriorhodopsin
1.3 Membrane Transport: Principles of membrane transport, ion 03
channels and electrical properties of membranes. protein transport,
post translation transport and co translation transport, protein
transport and protein secretion pathways in bacteria. endocytosis
1.4 Intracellular Compartments and protein sorting: 03
Compartmentalization of cells, transport of molecules between the
nucleus and cytosol, peroxisomes, Endoplasmic reticulum transport
of proteins into mitochondria and chloroplasts

1.5 Cell wall and extracellular matrix: matrix proteins, matrix 02

polysaccharides
1.6 Cell-cell interaction: 02

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Unit/Subunit Topics 15
Unit 2 Organization and Function of Mitochondria, Chloroplast and
Cytoskeleton
2.1 Mitochondria: Structure, electron-transport chains and proton 05
pump, Chloroplasts: Structure, energy capture from sunlight, genetic
system of Mitochondrion and chloroplast
2.2 Intracellular vesicular traffic: The molecular mechanism of 05
membrane transport and the maintenance of compartmental
diversity, transport from the ER through the Golgi apparatus,
transport from trans Golgi network to lysosomes
2.3 Cytoskeleton: The self-assembly and dynamic structure of 05
cytoskeletal filaments, How cells regulate their cytoskeletal filament,
(Cytoskeletal filaments, Microtubules, Actin and intermediate
filaments), molecular motors, cell behaviour

Unit/Subunit Topics 15
Unit 3: Title of Module: Cell Division, Cell Cycle and Cell Junctions
3.1 Mechanism of cell division: M-phase, Mitosis, Cytokinesis, Germ 04
cells and fertilization, Meiosis, eggs, sperm, fertilization
3.2 Cell cycle and Programmed cell death: Control system, 05
intracellular control of cell cycle events, Apoptosis, extracellular
control of cell growth and apoptosis. Autophagy
3.3 Cell Junctions and cell-cell adhesion: Anchoring, adherence 06
junctions, Desmosomes, Gap junctions, cell-cell adhesion,
Cadherins, Selectins, N-CAM, The extracellular matrix of animals.,
Integrins

Unit/Subunit Topics 15
Unit 4: Title of Module: Cell Communication and Multicellular Cell
Development
4.1 Cell communication: General principles of cell communication 04
(Nitric oxide gas signal and nuclear receptors, Three classes surface
receptors), Signalling through G-protein linked cell surface receptors
4.2 Signalling through enzyme linked cell surface receptors: 06
Tyrosine kinase, Docking sites, Ras, MAP kinase, Pl-3 kinase, TGF-

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 β, Signaling pathways by regulated proteolysis. Signalling in plants:
Serine / Threonine kinases, role of ethylene, Phytochromes
4.3 Development of multicellular organisms: Caenorhabditis elegans, 05
Drosophila melanogaster, Mouse Neural development

Course Code: PSC3MI-3

Course Title: Practical’s based on the theory syllabus of Cell Biology

Course Type: -Major Credit: -01

CO No. COs Statement

After completing the Bachelor of Science Program, students will be able to-
CO-1 Conduct qualitative and quantitative analysis of viable cells.
CO-2 Employ the isolation techniques to separate the lymphocytes.
CO-3 Demonstrate the separation of cellular proteins by electrophoresis.
CO-4 Understand the separation techniques for biomolecules.
CO-5 Analyse the effect of photons onto the photophosphorylation activity of chloroplast.
CO-6 Accurately perform the MTT and neutral red assay.

Experiments: -

SR. Experiments based on PSC3MI-3: -

NO.
1. Disruption of bacterial cells followed by characterization of cellular proteins by SDS-
PAGE
2. Isolation of lymphocytes and its Viability staining using trypan blue

3. Determination of cell viability of lymphocytes by MTT Assay& neutral red uptake assay

4. Comparison of various cell viability techniques.

5. Isolation of mitochondria and check activity of respiratory enzyme succinate

dehydrogenase
6. Isolation of chloroplast and checking photophosphorylation

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 References:-

SR. References
NO.
1. Molecular Biology of the Cell – Alberts, Johnson, Lewis, Raff, Roberts &Walter, 6th
edition.
2. Cooper, G.M., Hausman R.E. (2009) The Cell: A Molecular Approach- 6th edition.

3. Molecular Cell Biology. Harvey Lodish; Arnold Berk; Chris A. Kaiser; Monty
Krieger; Anthony Bretscher; HiddePloegh; Angelika Amon; Kelsey C. Martin , 8th
edition
4. Karp G. (2008). Cell and Molecular Biology: Concepts and Experiments. John Wiley
& Sons.
5. Gilbert , Barresi (2016) Developmental Biology : 11th Edition

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