Chaperone Plasmid Set

Cat.# 3340
v.0401

Table of Content

I.

Description ......................................................................................... 2

II.

Component ........................................................................................ 2

III.

Storage .............................................................................................. 4

IV.

Usage method ................................................................................... 4

V.

Reference .......................................................................................... 8

NOTICE: This product is manufactured and sold by TAKARA BIO INC. under license from HSP Research Institute, Inc. This product is intended for research purposes only. Use of this product for commercial purposes such as screening or production requires a separate commercial contract with HSP Research Institute, Inc. The sequence information on the plasmids supplied in this set are not disclosed, and it is prohibited to modify this product or to use this product for plasmid preparation without prior permission of HSP Research Institute, Inc.

URL:http://www.takara-bio.co.jp

TAKARA BIO INC.

Chaperone Plasmid Set

Cat.# 3340
v.0401

Proteins have been gaining attention as one of the challenges in the area of post-genomics, with emphasis being placed on analyses of their structures and functions. Large-quantity expression of recombinant proteins is essential for structural and functional analyses of interest and, to support this need, a variety of protein expression systems have been developed. Escherichia coli is commonly used as a host for protein expression, since it represents a simple system that offers a wide choice in expression systems. However, expression of foreign proteins in E. coli often results in various problems, such as the formation of inclusion bodies and protease degradation of the protein. These issues often are a result of improper folding of the expressed proteins, and are problems frequently encountered in protein function research. Molecular chaperones have been demonstrated to be involved in the protein folding process, and numerous studies have been conducted to elucidate the mechanisms of in vivo protein folding. HSP Research Institute, Inc. has made significant contributions in the area of chaperone research. Takara's Chaperone Plasmid Set consists of five different types of "chaperone team" plasmids developed by HSP Research Institute, Inc., which are designed to enable efficient expression of multiple molecular chaperones known to work in cooperation in the folding process. It has been reported that coexpression of a target protein with one of these chaperone teams increases recovery of expressed proteins in the soluble fraction. Such proteins are often unrecoverable using conventional methods due to the formation of inclusion bodies (see Figure 1).

II. Component

1. 2. 3. 4. 5.

Plasmid Plasmid Plasmid Plasmid Plasmid

pG-KJE8: pGro7: pKJE7: pGTf2: pTf16:

10 ng/l 10 ng/l 10 ng/l 10 ng/l 10 ng/l

100 100 100 100 100

l l l l l

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Chaperone Plasmid Set

Cat.# 3340
v.0401

Resistant Marker Cm

References 2,3

araB Pzt1 araB araB Pzt1 araB

Cm Cm Cm Cm

2 2 3 3

<Available E. coli Expression Systems> These chaperone plasmids carry an origin of replication derived from pACYC and a chloramphenicol resistance gene (Cmr). This arrangement allows their use with E. coli expression systems that utilize ColE1type plasmids containing the ampicillin resistence gene as a marker, which are the most commonly used. The chaperone genes are situated downstream of either the araB or Pzt-1 (tet) promoter. Thus, expression of target proteins and chaperones can be induced individually if the target gene is placed under the control of another promoter (e.g. lac). These plasmids also contain either araC or tetR for each promoter. Note that this system cannot be used in combination with chloramphenicol-resistant E. coli host strains or expression plasmids that carry the chloramphenicol-resistance gene. For example, E. coli BL21(DE3), which is often used with pET systems etc., is acceptable as a host strain, but E. coli BL21(DE3) pLysS or BL21(DE3) pLysE which contain either the pLysS or pLysE plasmids containing the pACYC replication origin and the Cmr gene, cannot be used with this system. Other applicable hosts include E. coli JM109.

URL:http://www.takara-bio.co.jp

TAKARA BIO INC.

Chaperone Plasmid Set

Cat.# 3340
v.0401

Tf Synthesized Protein

mRNA

DnaK DnaJ GrpE ATP

Proteolysis
GrpE ATP

ADP

Native Form Naive Form

Aggregation
ADP ATP

Proteolysis
GroEL GroES

Fig. 1

Possible model for chaperone - assisted protein folding in E. coli (Reference 1).

III. Storage IV. Usage method

-20C <Chaperone Co-expression> The optimum chaperones and culture conditions (e.g. medium, culture temperature, aeration conditions, timing of induction, inducer concentration, and induction period) vary depending upon the target protein. A usage example is shown below. Optimum conditions should be determined independently for each target protein.

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URL:http://www.takara-bio.co.jp

Chaperone Plasmid Set

Cat.# 3340
v.0401

1. Construction of a System for Co-expression An effective method for constructing a system for coexpression of target proteins and chaperones involves two steps: transformation of E. coli with a chaperone plasmid followed by transformation with an expression plasmid for the target protein. One-step methods, i.e. simultaneous transformation with a chaperone plasmid and an expression plasmid for a target protein, and two-step methods involving transformation with an expression plasmid followed by transformation with a chaperone plasmid are not recommended, as they are known to result in very low transformation efficiency. (1) Construct an expression plasmid for a target protein to be expressed in E. coli. (2) Prepare competent cells of an E. coli expression host using a standard method. (Commercially available competent cells may be used instead.) (3) Transform the competent cells prepared in (2) with one of the chaperone plasmids contained in this set, and select the transformants from selection medium plates containing 20 g/ml chloramphenicol (use approximately 1 l of plasmid for each transformation.) (4) Culture the transformants with the chaperone plasmid in liquid medium containing 20 g/ml chloramphenicol and prepare the competent cells using a standard method. (5) Retransform the competent cells prepared in (4) with the expression plasmid for the target protein, and select the transformants from selection medium plates containing 20 g/ml chloramphenicol and a selection reagent for the expression plasmid.

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TAKARA BIO INC.

Chaperone Plasmid Set

Cat.# 3340
v.0401

2. Co-expression Experiment The experiment shown below is an example of a co-expression experiment which uses a pUC plasmid carrying the ampicillin resistance marker as well as a target gene downstream of the lac promoter, with a chaperone plasmid from this set. Refer to additional relevant literature for further details as to the appropriate culture conditions to use in your application. (1) To perform coexpression, inoculate the transformants into L medium containing 20 g/ml chloramphenicol and 50 g/ml ampicillin for plasmid selection and 0.5 - 4 mg/ml L-arabinose and/or 1 - 10 ng/ml tetracycline* for induction of chaperone expression. Incubate at 30 - 37C. Use both L-arabinose and tetracycline with pG-KJE8, L-arabinose only with pGro7, pKJE7, or pTf16, and tetracycline only with pG-Tf2. * Tetracycline at low concentrations does not significantly affect the growth of E. coli. (2) When the OD600 of the culture reaches 0.4 - 0.6, add IPTG to be a final concentration of 0.1 - 1 mM, and culture at 30 - 37C for 1 - 4 h. (3) After culturing, determine the amount of the target protein expressed and the amount of target protein in soluble form by SDS-PAGE and activity assay, Additionally, test various culture conditions (e.g. medium type, culture temperature, aeration conditions, timing of induction, inducer concentration, and induction period) to determine optimal conditions.

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URL:http://www.takara-bio.co.jp

Chaperone Plasmid Set

Cat.# 3340
v.0401

Cm r araC

Cm r

araB dnaK

groEL
pACYC ori araC

pG-KJE8 groES (11.1 kbp) Pzt-1

pGro7 (5.4 kbp)

dnaJ

araB groES

grpE
rrnBT1T2

Pzt-1
Cm r pACYC ori araC Cm r

pACYC ori

groES

araB

pKJE7 (7.2 kbp)

grpE

pG-Tf2 (8.3 kbp)

groEL

dnaK

dnaJ

tig

araB
araC

Fig. 2

Chaperone plasmids

tig

pTf16 (5 kbp)
Cm r

pACYC ori

URL:http://www.takara-bio.co.jp

TAKARA BIO INC.

Chaperone Plasmid Set

Cat.# 3340
v.0401

Reviews 1. Thomas, J. G., et al.(1997) Molecular Chaperones, Folding Catalysts, and the Recovery of Active Recombinant Proteins from E.coli. Appl. Biochem. Biotech, 66:197-238 Expression of soluble recombinant proteins through coexpression with chaperones 2. Nishihara, K., et al.(1998) Chaperone Coexpression Plasmids: Differential and Synergistic Roles of DnaK-DnaJ-GroE and GroEL-GroES in Assisting Folding of an Allergen of Japanese Cedar Pollen, Cryj2, in Eschericia coli. Appl. Environ. Microbiol . 64:1694-1699 3. Nishihara, K., et al.(2000) Overexpression of Trigger Factor Prevents Aggregation of Recombinant Proteins in Escherichia coli. Appl. Environ. Microbiol.66:884-889

NOTE: For research use only. Not for use in diagnostic or therapeutic procedures.

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