Cell Culture Laboratory
Cell Culture Laboratory
Cell Culture Laboratory
(Lec I)
Dr. Anum Gul
General principles for Cell culture Lab Design
Cover the floor with a vinyl, acrylic coating, or other dustproof finish,
and allow a slight fall in the level towards a floor drain located outside
the door of the room (i.e., well away from the sterile cabinets).
General principles for Cell culture Lab Design
If you have a large tissue culture lab with a separate washup and
floor as, and adjacent to, the laboratory, with no steps to negotiate,
traffic.
Layout depends on the type and scale of the operations and the number
of users.
Requirements
Number of users: How many people will work in the facility, how long will
they work each week, and what kinds of culture will they perform?
Space: What space is required for each facility? The largest area should
area.
Requirements
The second largest is for washup, preparation and sterilization, the third is for
Facilities for washing up and for sterilization should be located (a) close to the
aseptic area that they service and (b) on an outside wall to allow for the
possibility of heat extraction from ovens and steam vents from autoclaves.
Requirements
culture area within a larger laboratory can still be used for sterile handling,
Water bath
Centrifuge
Hemocytometer
Inverted microscope
Three kinds of cell culture hoods, designated as Class I, II, and III, have
They may or may not be ducted outside. Class I cabinets are safe
for use with agents requiring Biosafety Level 1, 2 or 3 containment.
Biosafety Cabinet Class I
Biosafety Cabinet Class II
Class II BSCs are designed with an open front with inward airflow
protection).
Biosafety Cabinet Class II
Type B1 Type B2
Biosafety Cabinet Class II
Biosafety Cabinet Class III
The cabinet has a transfer chamber that allows for sterilizing materials
before they leave the glove box.
Biosafety Cabinet Class III
The exhaust air is treated with either double HEPA filtration or HEPA
Class III cabinets are safe for work requiring Biosafety Level 1, 2, 3 or
4 containment.
Biosafety Cabinet Class III
Air-flow characteristics of cell culture hoods
Cell culture hoods protect the working environment from dust and
can be vertical, blowing from the top of the cabinet onto the work
surface.
Clean benches
filtered air from the back of the cabinet across the work surface
toward the user, and they may expose the user to potentially
hazardous materials.
Clean benches
Clean benches
time, be easily cleanable inside and outside, have adequate lighting, and
Keep the work space in the cell culture hood clean and uncluttered, and
Disinfect each item placed in the cell culture hood by spraying them with
There are two basic types of incubators, dry incubators and humid CO2
incubators.
Dry incubators are more economical, but require the cell cultures to be
Placing a water dish in a dry incubator can provide some humidity, but
incubator.
Incubator
Humid CO2 incubators are more expensive but allow superior control
of culture conditions.
culture area within a larger laboratory can still be used for sterile
non-laboratory areas.
As a general rule, air should flow from low hazard to high hazard
areas.
Ventilation Considerations for Biosafety Level 2
Laboratories
Tissue culture rooms that involve the use of biohazardous agents shall
be negative.
Thank You