Data Budidaya Tambak Jember Indah

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DATA BUDIDAYA TAMBAK JEMBER INDAH

Produk CV. Duta Wira Karya yang di gunakan :

- Indolacto : Merupakan formulasi probiotik yang digunakan untuk meningkatkan


produktivitas dan ekosistem perairan budidaya
 Lactobacillus casei 1×10^6 CFU/ml
 Lactobacillus delbruckil 1×10^6 CFU/ml
 Lactobacillus paracaseii 1×10^6 CFU/ml
 Lactobacillus rhamnosus 1×10^6 CFU/ml
- Indodine : Disenfektan berbahan dasar iodine 20%

Tebar : 25 January 2023


Total Benur : 1.100.000 Ekor
Benur : BBD
Kolam : Bulat Diameter 32m
Kolam : HDPE
Luasan : 803,84 m2
Padat tebar : 274 ekor/Meter

Case : AHPND 18 hari


Survive : 65 hari

Sistem pengunaan Bakteri


Dosis : 0.5 ppm/luas kolam
Media : 25 liter air
Aplikasi : 15 ppm/ 2hari
Kecerahan : 100-50 pagi + mineral
40-20 sore + mineral
Campuran pakan
Dosis : 3ml/kg
Total pakan : 11 ton
Tebar Kolam : 15ppm/2hari

Bakteri yang di Peraman


Dosis : 2 Liter/media 25L
ABW ABW ESTIMASI PAKAN
PETAK LUAS TEBAR TANGGAL DOC HARI ADG SIZE P/H INDEX POPULASI SR FCR KG/M
SEBELUM SEKARANG BIOMASS KMLTF
A1 706 220,000 30 2.96 30 0.10 337.8 19.3 0.56 658 222,200 101% 660 1.00 0.80
A2 706 220,000 30 3.01 30 0.10 332.2 19.3 0.57 656 217,800 99% 660 1.01 0.79
A3 706 220,000 24-Feb 30 3.03 30 0.10 330.0 19.3 0.57 660 217,800 99% 660 1.00 0.80
A4 706 220,000 30 2.77 30 0.09 361.0 19.3 0.52 658 237,600 108% 660 1.00 0.78
A6 706 220,000 30 3.47 30 0.12 288.2 19.3 0.66 657 189.200 86% 660 1.01 0.79
2,790 2,755 0,99

ABW ESTIMASI PAKAN


PETAK LUAS TEBAR TANGGAL DOC ABW SKRG HARI ADG SIZE P/H INDEX POPULASI SR FCR KG/M
LALU BIOMASS KMLTF
A1 706 220.000 37 2.96 5.02 7 0.29 199.2 45.0 0.60 1,115 222,200 101% 975.0 0,87 1.15
A2 706 220.000 37 3.01 5.21 7 0.31 191.9 48.0 0.65 1,135 217,800 99% 996.0 0.88 1.11
A3 706 220.000 03-Mar 37 3.03 5.18 7 0.31 193.1 48.0 0.65 1,128 217,800 99% 996.0 0.88 1.16
A4 706 220.000 37 2.77 4.98 7 0.32 200.8 50.0 0.62 1,183 237,600 108% 1,010.0 0.85 1.16
A6 706 220.000 37 3.47 5.50 7 0.29 181.8 45.0 0,67 1,089 198,000 90% 989.0 0.91 1.14
4,035 4,121.0 1.02

ABW ESTIMASI PAKAN


PETAK LUAS TEBAR TANGGAL DOC ABW SKRG HARI ADG SIZE P/H INDEX POPULASI SR FCR KG/M
LALU BIOMASS KMLTF
A1 706 220.000 44 5.02 6.12 7 0.16 163.4 54.0 0.59 1,360 222,200 101% 1,353.0 0.99 1.24
A2 706 220.000 44 5.21 6.30 7 0.16 158.7 54.0 0.60 1,372 217,800 99% 1,374.0 1.00 1.33
A3 706 220.000 10-Mar 44 5.18 6.26 7 0,15 159.7 53.0 0.59 1,363 217,800 99% 1,367.0 1.00 1.47
A4 706 220.000 44 4.98 6.54 7 0.22 152.9 62.0 0.64 1,554 237,600 108% 1,444.0 0.93 1.46
A6 706 220.000 44 5.50 7.37 7 0.27 135.7 58.0 0.71 1,459 198,000 90% 1,395.0 0.96 1.50
4,939 5,957.0 1.21
Lactobacillus
The ability to metabolize citrate to CO2, acetate, acetoin, diacetyl and sometimes 2,3-butanediol is a
common trait of many lactic acid bacteria (LAB), especially Lactococcus and Leuconostoc spp.

Growing cells utilized citrate faster when co-metabolizing galactose than when co-metabolizing
glucose or lactose but grew more slowly on galactose

Greater metabolism of citrate occurred if the cells were grown on galactose than on glucose or
lactose. This may be due to repression of citrate metabolism by glucose. This effect is on enzyme
synthesis rather than on enzyme activity. Is the one normally found in other LAB viz. citrate :
oxalacetate : pyruvate : acetolactate : acetoin/diacetyl (Hugenholtz 1993).

Palles, P., Beresford, B., Condon, C., & Cogan, C. (1998). Citrate metabolism in Lactobacillus casei and Lactobacillus
plantarum. Journal of Applied Microbiology, 85(1), 147-154.

The immunomodulatory role of lactic acid bacteria

There are several immune responses induced in shrimp like physical barriers, encapsulation, bacteria
clearance, antimicrobial activity, pro-phenol-oxidase system, clotting reactions, and reactive oxygen
intermediates. The innate immune response was induced under a diversity of PRR (pattern
recognition receptors) on the hemocyte membranes to discover the PAMP (pathogen-associated
molecule pattern) and reduce signals during the attack of pathogens. Then, hemocytes are activated,
and the defense molecules are degranulated against the defined pathogens (Song and Li 2014).

This may be attributed to the role of L. plantarum in activation of the pro-phenol-oxidase (Pro. PO),
phenol-oxidase (PO), and superoxide dismutase (SOD) activities, respiratory bursts (RB), and
peroxinectin mRNA transcription and increased the rate of survival after V. alginolyticus challenge
(Chiu et al. 2007). Also, Lactobacillus bulgaricus improved both the humoral and cellular immune
defense activities in the white shrimp, Litopenaeus vannamei, in terms of increasing hemocyte
counts, PO, and RB activity and reduced shrimp mortality after challenged with V. parahaemolyticus
(Roomiani et al. 2018). Huynh et al. (2018) suggested that Lactobacillus plantarum could modulate
the immune system of Litopenaeus vannamei by increasing the levels of several metabolites such as
inosine monophosphate (IMP), valine, and betaine.

the antiviral affects may be attributed to the interaction between bacterial cell and virus, to the
ability of bacterial cell to produce the antiviral bacterial derivatives, or to the immunomodulatory
role of probiotic towards activation of the immune system The interaction between bacterial cell
and virus could be possible through absorbed and/or trapped mechanisms which inhibited virus’s
proliferation.
Nguyen et al. (2018) presented the evidence on the positive effects of the probiotic Lactobacillus
plantarum on growth and resistance of Litopenaeus vannamei against Vibrio parahaemolyticus
which causes acute hepatopancreatic necrosis disease (AHPND).

With regard to the harmful effects of ammonia, Liu and Chen (2004) stated that ambient ammonia
reduced the resistance of Litopenaeus vannamei to Vibrio infection by decreasing the phagocytic
and SOD activities and increasing the superoxide anions which may be toxic to the host (Ma et al.
2009).

The higher concentration of heterotrophic bacteria led to rapidly convert dissolved free nitrogen
into natural microbial biomass. Actually, heterotrophic bacteria can be developed fastly up to 10
times more than nitrification bacterium and thus markedly minimized the time required to remove
the toxic from of ammonia-N (reduced nitrite-N levels) (Hargreaves 2006).

Naiel, M. A., Farag, M. R., Gewida, A. G., Elnakeeb, M. A., Amer, M. S., & Alagawany, M. (2021). Using lactic
acid bacteria as an immunostimulants in cultured shrimp with special reference to Lactobacillus spp. Aquaculture
International, 29, 219-231.

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