Product Manual

Absorbance 96 plate reader

Catalog #: ENZ-INS-A96
Manufactured by Byonoy GmbH
Version 1.4 (2020-07-16)

For the latest product information, including support documentation, visit us

online:
www.enzolifesciences.com

Technical Support (US): 800-942-0430

Technical Support (EU): +41 61 926 8989

Customer Service (US): [email protected]

Customer Service (EU): [email protected]

FOR RESEARCH USE ONLY.

NOT FOR USE IN DIAGNOSTIC PROCEDURES.

Rev 102320
Instructions for Use
Absorbance 96
Software Version 1.1.0

Instructions for Use

Absorbance 96, Absorbance 96 App
Version 1.4 (2020-07-16)
©Copyright 2020 by Byonoy GmbH. All rights reserved.

Manufacturer: Byonoy GmbH 00 49 40 5379 866 00

Bernstorffstr. 118 [email protected]
22767 Hamburg, Germany www.byonoy.com
Dear customer,
We are delighted that you have chosen the Absorbance 96. To take full ad-
vantage of the instrument's performance and to enjoy your analysis instrument
for many years, please read these instructions for use carefully before installation
and commissioning. Operate the instrument in accordance with these instruc-
tions. The operating safety and function of the instrument can only be guaran-
teed if both general safety regulations and accident prevention regulations of
the legislator as well as the safety instructions in this manual are regarded. We
accept no liability for any damage resulting from improper use or incorrect oper-
ation.

I Ensure that the manual is accessible at all times and is read and un-
derstood by all persons operating the instrument. Keep this manual in
a safe place so that you can access it when necessary.

I The latest version of this manual (instructions for use) is available via
the Absorbance 96 App. Upon request, a paper version can be
shipped within 7 business days. Please use the contact form on the
website.

This user manual may only be used according to its intended purpose. It may not
be reproduced, changed, or translated in another language without prior written
consent of Byonoy GmbH.

This document is subject to technical changes and updates.

2
TABLE OF CONTENTS

1 General information ..................................................................................................................... 4

2 Instructions for using the Absorbance 96 ..................................................................... 6

2.1 Scope of application .................................................................................................... 6

2.2 Measurement method ................................................................................................. 6
2.3 About the Absorbance 96 .......................................................................................... 6
2.4 Information on consumables ..................................................................................... 8
2.5 Power consumption .................................................................................................... 8

3 Preparing the product for use .............................................................................................. 9

3.1 Unpacking, storage and transport ........................................................................... 9

3.2 Checking the supplied parts ..................................................................................... 9
3.3 Working environment ............................................................................................... 10
3.4 Software Installation Procedure & Updates ........................................................ 10

4 Operation ...........................................................................................................................................11

4.1 Connecting the Absorbance 96 with the computer .......................................... 11

4.2 Setup an assay and create a protocol .................................................................. 11
4.3 Running the assay ...................................................................................................... 17

5 Information required by the user ...................................................................................... 21

5.1 Explanation of the signal lights ............................................................................... 21

5.2 Notes on troubleshooting ........................................................................................ 21

6 Maintenance and cleaning ................................................................................................... 23

6.1 Maintenance................................................................................................................ 23
6.2 Cleaning ........................................................................................................................ 24
6.3 Technical support ...................................................................................................... 26
6.4 Repairs .......................................................................................................................... 26

7 Performance data and technical data ........................................................................... 27

8 Guarantee ....................................................................................................................................... 28

EC-Conformity Declaration ........................................................................................................... 29

3
 General information

1 General information

Important: Follow the instructions for use

Any commissioning or handling of the instrument requires precise knowledge and notice of
this manual. The device is intended for the described use only.

In this manual, particularly important remarks are highlighted as followed:

Warning:
!! This is a warning that indicates an immediate or potentially dangerous situation,
which, if not avoided, will result in death or serious injury.

Caution:
! Caution indicates a potentially hazardous situation, which, if not avoided, could
result in minor or moderate injury.

Information:
I This is a piece of information indicating certain characteristics that must be ob-
served.

Liability for function and damage

Liability for the function of the device shall in any case pass to the owner or operator if the
device is improperly maintained, repaired, or modified by persons who do not belong to the
authorized service personnel, or if it is handled in a way that does not comply with its in-
tended use. The service and operation of the product must be in accordance with this man-
ual. Byonoy GmbH shall not be liable for damages resulting from non-observance of the in-
formation above. Warranty and liability conditions of the terms of sale and delivery of Byonoy
GmbH are not extended by the information above.

Warning

!! An incorrect working environment can lead to a reduction in service life, damage

to the device, or measurement errors. Please note the explanations and warn-
ings in chapter 3.3.

!! To avoid measurement errors, it is essential to ensure that the instrument is con-

nected properly and that the experiments are carried out correctly. Please note
the explanations and warnings in chapter 3.

!! Incorrect cleaning of the instrument can reduce its service life and can cause
damage to the instrument. Please note the explanations and warnings in chapter
6.2.

!! To avoid the risk of electric shock, do not expose the instrument to rain or ex-
cessive moisture and do not touch the instrument or USB cable with wet hands.

4
 General information

Information

I Follow all safety instructions on the device and in the attached documents.

I Follow all general precautions that apply to electrical instruments.

Disposal of used electrical and electronic equipment

The symbol on the product or its packaging indicates that this product is not to be treated
as normal household waste. It must be disposed of at a collection point for the recycling of
electrical and electronic equipment. By contributing to the correct disposal of this product,
you protect the environment and the health of your fellow human beings. The environment
and health are endangered by incorrect disposal. Material recycling helps to reduce the con-
sumption of raw materials. For further information on how to recycle this product, please
contact your local authority or municipal waste disposal companies.

Working with biological and harmful material

The Absorbance 96 is not to be used for the measurement of biohazardous substances.

Always observe the manufacturer's hazard information pertaining to the substances to be

measured.

The instrument does not produce any toxic or harmful gases or substances. During the
measurement, make sure that there are no toxic or harmful substances in the microtiter
plate.

5
 Instructions for using the Absorbance 96

2 Instructions for using the Absorbance 96

2.1 Scope of application

The Absorbance 96 is an absorbance-based measurement device, i.e. a measuring instru-

ment with which the absorbance values of the contents of a 96-well microtiter plate can be
read, recorded and further processed. With the Absorbance 96, ANSI/SBS Standard 96-well
microtiter plates can be read. The Absorbance 96 is intended for research and other non-
in-vitro-diagnostic analyses only. It is to be operated by trained laboratory personnel and is
intended for professional use.

The Absorbance 96 App is a microplate-reader-control software. It is intended to be used

for the readout and analysis of data produced by the Byonoy Absorbance 96 microplate
reader.

2.2 Measurement method

The Absorbance 96 is designed to carry out fast and sensitive absorbance measurements.
It measures the optical density (OD) of samples at defined wavelengths.

Absorption

Absorption refers to the amount of light absorbed by a medium. Absorption reduces trans-
mission. Transmission is the ratio of incident to transmitted light. Accordingly, the degree of
transmission is calculated as follows

T = (I/I0)

where I is transmitted light and I0 is incident light.

Optical Density

Optical density is a measure of the attenuation of light radiation after it has passed through
a medium. Optical density is the logarithmic quantity that describes the reciprocal of the
transmittance T:

OD = log(I0/I)

where I is the transmitted light and I0 is incident light.

Optical density is the absorbance of the sample plus other attenuating effects such as scat-
tered light due to turbidity. It is therefore necessary to avoid scattered light in order to meas-
ure absorbance correctly.

If no other attenuating effects are present, absorbance = optical density.

2.3 About the Absorbance 96

The Absorbance 96 is an absorbance-based measuring device, i.e. a measuring instrument,

with which the absorbance values of a 96-well microtiter plate can be read, recorded and

6
 Instructions for using the Absorbance 96

provided for further processing. The Absorbance 96 has 96 detection units, which allow
measurement without a scanning mechanism.

In the upper part of the measuring instrument there are 4 light sources with different wave-
lengths and 4 interference filters. The four signal lights on the top of the reader show which
measuring channel is being used for the current measurement.

The microtiter plate is inserted manually into the slot of the device. There are two raised
locking-edges at the bottom of the slot, as well as an arrow for visual inspection that be-
comes wholly visible only after the microtiter plate has been completely inserted.

Figure 1. Visual representation of the product from the front, top. 1. Signal lights. 2. Slot for
microtiter plate. 3. Raised locking-edges. 4. Arrow.

On the back of the Absorbance 96 is a USB plug for connecting the instrument to a computer
via the included Micro-USB cable. Furthermore, there is an eject button for ejecting the mi-
crotiter plate out of the slot.

Figure 2. Visual representation of the product from behind, top. 5. Eject button. 6. Micro-
USB plug.

7
 Instructions for using the Absorbance 96

2.4 Information on consumables

With the Absorbance 96, ANSI/SBS Standard 96-well microtiter plates can be read.

When using microtiter plates, always regard the specifications of the manufacturer of the
microtiter plates. Not all microtiter plates of a manufacturer are the same in design, materials,
or configuration. The temperature stability within the microtiter plate can depend on the
type of microtiter plate used.

2.5 Power consumption

The Absorbance 96 is an analytical instrument with very low power consumption. It is oper-
ated by a Micro-USB cable and the total power consumption is generally less than 2.5 watts.

8
 Preparing the product for use

3 Preparing the product for use

3.1 Unpacking, storage and transport

Unpacking

Remove the packaging material and carefully place the unit on a firm and level surface.

Check the parts supplied (see chapter 3.2).

! Check the product for external damages.

! Make sure, that the rubber feet are completely attached to the underside of the de-
vice.

The device is packed in a specially designed cardboard box. Keep the packaging material. If
you return the device for repair, the original packaging material and box must be used.

Storage and protection during the intervals of normal use

!! Protect the device from moisture and dust during prolonged storage.

! Do not store the device next to heat sources and protect it from direct sunlight.

The storage temperature should be between 5°C and 45 °C.

Transportation

Before transportation, make sure that the device is disconnected from the computer and
that it is turned off. Remove the cable from the instrument and make sure that there is no
microtiter plate in the instrument. Depending on the transport distance, use the original
packaging material. Make sure that the new location meets the requirements described in
chapter 3.3.

! Always transport the product well packed to avoid possible transport damage.

!! Before transport, make sure that there is no microtiter plate in the

instrument.

3.2 Checking the supplied parts

When unpacking the device, please check that the following components are present:

• Absorbance 96
• Micro-USB cable
• Hex key
• Microfiber cloth
• USB Stick

9
 Preparing the product for use

3.3 Working environment

Due to its small size, the Absorbance 96 can be operated very well in various working envi-
ronments.

However, the following warnings must be regarded. Ignoring them may lead to a reduction
in the expected lifetime, damage to the device, or measurement errors:

!! The device should stand on a level surface, free from dust and vibrations.

!! Do not operate the device near heat sources or under direct sunlight.

!! The ambient temperature should be between 5°C and 45°C.

!! The maximum humidity should not exceed 70%.

3.4 Software Installation Procedure & Updates

To install the Absorbance 96 App, insert the USB stick provided with the Absorbance 96
Reader into the computer and follow the steps below:

• Locate and select the USB drive in the document browser.

• Open the installation file either for Windows or macOS.
• Follow the steps in the Setup Wizard to complete installation.

I To install the proper drivers and software on the computer, the user must have ad-
ministrator rights, or obtain such permissions.

I For Windows PCs: The computer must have at least windows 7 or above. Windows
10 and later versions function through plug and play. If an older version is being
used, drivers may need to be installed. These are available on the USB stick pro-
vided with the device.

Uninstallation of software

The Absorbance 96 App can be uninstalled using the computer’s standard uninstall proce-
dure.

Updates

With Internet Access:

To check for new updates, click the update button in the menu bar. If an update is available,
this will be shown in a bar at the top of the screen.

Without Internet Access:

1. Uninstall the current version of the software.
2. Load the update file using the link provided by Byonoy.
3. Open the file on the computer. The new software should now be installed.

10
 Operation

4 Operation

Before commissioning the Absorbance 96, you should carefully read and understand the en-
tire manual, so that you are familiar with the system.

4.1 Connecting the Absorbance 96 with the computer

! Place the instrument in a horizontal position secured against falling.

The Absorbance 96 requires a connection to the computer for power supply, to start the
experiment, and for data transfer. Insert the Micro-USB cable into the Absorbance 96 and
the USB port of the computer. The Absorbance 96 starts automatically when connected to
a computer via the Micro-USB cable and the software automatically establishes a connec-
tion.

!! Always use the included Micro-USB cable for the connection to your computer.

I Connect the instrument directly to your computer and do not use an external USB
hub.

I The device may only be operated at a USB port USB 2/USB 3 with 5 VDC and a
maximum of 3 A of a certificated computer (with certification mark of an approved
testing laboratory). Use an adapter cable on USB 3.1 with type C plug connection,
which ensures the profile 1: 5 [email protected] A.

When the Absorbance 96 is switched on, the signal lights on the top of the instrument light
up. Each time the Instrument is switched on, an internal self-test is performed to ensure there
are no malfunctions.

If no connection between the instrument and the computer is established, install the driver
for the USB connection. You can find it in the supplied USB Stick. See chapter 3.4.

4.2 Setup an assay and create a protocol

Select “New” from the menu bar. Your experiment conditions can now be defined.

Name and Describe a Protocol

In the first section of the Setup Page you can name your assay/protocol. For the protocol
you can additional add a description, assign a supplier for the assay kit, or choose a logo.

Workflow Parameters

Wavelengths

Under “Wavelength”, a signal and reference wavelength may be chosen.

The signal wavelength is the wavelength (in nanometers) at which the principal measure-
ment will be run.

11
 Operation

The reference wavelength is the wavelength (in nanometers) at which the reference is
measured. The values measured at the reference wavelength will be subtracted from the
values measured at the signal wavelength.

If no reference wavelength is desired, click the “None” button beside the reference wave-
length.

Figure 3. Experiment Parameters

Readout Method

Under “Method”, the readout method can be selected.

In an “Endpoint” measurement, one measurement cycle is performed. Here you can choose
between the different evaluation methods described below.

In a “Kinetic” measurement, you can set the parameters for multiple measurement cycles
taken over a given time span.

I To perform a signal-stable kinetic measurement, please ensure constant environ-

mental conditions. I.e. constant ambient temperature and humidity.

I For best results, acclimatize the connected reader and plate to the ambient temper-
ature for at least 1 h.

12
 Operation

I With kinetic measurements, a signal drift of about 1 mOD per hour might be observed.

Evaluation

The evaluation section allows you to choose which evaluative method will be used for the
readout.

No Evaluation

No evaluation will be used. The raw OD values will later be shown in plate format.

Quantitative

• Upon selection of the Quantitative evaluation method, a second section titled “Fitting
Method” will appear in the evaluation field.
• Click the drop-down menu and select one of the options: point-to-point, linear re-
gression, 4-parameter or 5-parameter regression.
• The selected evaluation method will be used for the standard curve and the data
analysis.

Qualitative

Upon selection of qualitative evaluation, the plate layout window will change and a formula
for the cut-off definition will appear.

Terms for Cut-Off Definition

POS = the mean of the OD values of the positive control wells.

NEG = the mean of the OD value of the negative control wells.
CUT = the mean of the OD value of the cut-off control wells.
0 OD = zero. User defined value may be entered. See example 3.
Offset = the offset value for cut-off calculation.
Range = a percentage value defining a range beyond which the results are interpreted as
either positive or negative.

The formula setup for the cut-off definition covers multiple formula types. These are listed
below.

1. Cut-Off Value = Mean Neg. Controls +(-) k

2. Cut-Off Value = Mean Pos. Controls + k
3. Cut-Off Value = Mean CO. Controls + k
4. Cut-Off Range = [Mean (x)control + k] +/- x%

Where k is an experimentally determined factor normally defined by the laboratory profes-

sional or by the kit manufacturer.

Example 1: Subtraction

To replace the minus function, the user must simply enter a negative value. For example, if
the cut-off is:

13
 Operation

Cut-off = Mean Neg. Controls - 0.2

this must be entered as follows:

Example 2: Range Definition

To define a range, you may use the “Range” field in the cut-off editor. This allows you to enter
a percentage that will correspond to values above and below a given cut-off value, within
which the experimental values will be deemed intermediate, above which positive, and below
which, negative.

For example:
Sample is considered positive if: SampleOD > (Mean Pos. + 10%)
Sample is considered negative if: SampleOD < (Mean Pos. -10%)
Sample is considered intermediate if: (Mean Pos. – 10%) < Sample OD < (SampleOD + 10%)

This would be entered as follows:

Example 3: Known Cut-Off Value

It may be the case that the cut-off value is known for a given experiment and need not be
calculated. In this case, the “0 OD” option may be selected for the calculation and the desired
value entered into the offset.

For Example: Cut-Off = 0.3

Then:

I The examples shown in this manual do not represent actual data, rather function to
clarify software features, and may not be referenced for experimental methods or
procedure.

Plate Layout

In the plate layout section, the microplate can be filled out as desired.

Each well may be defined individually using the buttons below the plate.

I The format of the plate layout and the corresponding buttons are defined by the
evaluation method. Quantitative and qualitative evaluations have different fields
and buttons.

I Blank values will be subtracted in the readout.

14
 Operation

I The mean value of replicates will be calculated

I Once the conditions/parameters of a given experiment have been set, these set-
tings may be saved as a protocol. All parameters, such as wavelength and plate
layout, will be saved.

I Wells without values (concentration or sample ID) are shown with a ring instead of
solid colour.

Quantitative Evaluation

When the quantitative evaluation is selected, the window will be as shown in figure 5.

Figure 4. Quantitative plate definition window.

To define a well, click on the desired button below the plate. You may then click and drag, or
individually define single wells. The blue ring indicates which field is currently selected; all
fields will be defined starting with the selected number.

To enter a concentration or sample ID, type the value in beside the number and press ENTER
to move to the next field.

To enter a unit, enter the desired unit in the first field and press ENTER, or choose a prede-
fined unit form the drop-down menu. All fields will be automatically filled out according to the
first selection.

To define, for example, two duplicate standard rows, click and drag the desired number of
wells, release, then click and drag again in the next row.

15
 Operation

To set a blank or to empty a given well, click on the “Blank” or “Empty” button respectively,
then click the desired well or drag and drop across the plate.

I When blank wells are selected, the values of these wells will be subtracted in the
readout.

Qualitative Evaluation

Upon selection of qualitative evaluation, the plate layout window will look like that shown in
figure 6. Define the plate according to the description above.

Figure 5. Qualitative plate definition window.

Use Predefined Standard Curve

In a quantitative assay, a predefined or previously measured standard curve can be used to

determine the concentrations of a new set of samples with identical experiment parameters.
After selecting the checkbox “Use Predefined Standard Curve”, you may enter the curve pa-
rameters manually or load a standard curve from a previous assay.

After selecting the checkbox “Use predefined Standard curve”, you cannot assign any new
standards.

! In a quantitative assay, the standard curve is a function of various assay parame-

ters, such as the temperature, timing, or age of the assay kit. Before using this
feature, it is advisable to validate your assay for compatibility.

I As the above-mentioned experiment conditions are subject to change, it is rec-

ommended to use a new standard curve when performing a new assay.

16
 Operation

File Types

The following file types are used by the Absorbance 96 App:

Table 1. File Types in the Absorbance 96 App

Quality Extension
Protocol .byop
Assay .byoa
Export .csv/.pdf

4.3 Running the assay

Procedure walkthrough

1. Empty Slot

Ensure the slot is empty for the next step.

2. Initialization

Click “OK” to start the initialization. The initialization is carried out, which is indicated by a
short illumination of the respective LEDs.

I The slot must be empty for initialization.

Figure 6. Prompt, guidance through the measurement steps.

17
 Operation

3. Insert Plate

After successful initialization, you will be prompted to insert the microtiter plate. Pay atten-
tion to the alignment of the microtiter plate. For orientation, a pictogram on the top of the
Absorbance 96 indicates the correct alignment of the microtiter plate. (The pictogram is a
schematic representation. The actual shape of the microtiter plate, especially the position of
the rounded corner, may vary and differ from the illustration. Base your alignment on the A1
Well).

After insertion, confirm in the software that the microtiter plate has been inserted by clicking
OK. The measurement will subsequently be carried out, accompanied by a short illumination
of the respective signal lights.

Figure 7. Insertion of the microtiter plate.

In order to guarantee a correct measurement result, please pay attention to the following
warnings:

!! When inserting the microtiter plate, pay attention to its alignment.

!! Make sure that the microtiter plate was inserted in the slot completely. In that case
the arrow at the bottom of the slot is visible completely.

!! The bottom of the microtiter plate must be dry before it is inserted into the instru-
ment. If the microtiter plate is wet at the bottom, dry it with a paper towel or similar.

!! Shocks of the Absorbance 96 and of the microtiter plate can cause an unwanted
signal. Therefore, neither the Absorbance 96 nor the microtiter plate should be
touched during measurement.

!! Note that the system depends on a clean and dust-free environment. Therefore, do
not wear powdered safety gloves.

!! Ensure that no direct sunlight or other strong light sources shine on the Absorb-
ance 96 and especially into its slot.

!! Insert the plate cautiously to avoid spills

3. Read Plate

18
 Operation

When the measurement is complete, the results will be shown.

You may now remove the microtiter plate from the Absorbance 96. To do this, push the eject
button on the rear side of the device.

Figure 8. Remove the microtiter plate.

Results and Interpretation

Plate Overview: Provides an overview of the different OD values in their respective wells.
Undefined wells are not shown. Click on a given well to see the OD, label and, if applicable,
concentration.

Graph Overview: A representation of the data will be shown on the graph. The curve will be
dependent on the data analysis method chosen and will be fitted accordingly. By choosing
the right regression method, the distance from the curve to each point is minimized. Using
the standard curve, the concentration of each sample is calculated. The equation below the
graph reflects the regression method applied and offers further data.

Tabular View: Provides an overview of the various samples, wells, ODs and calculations.

Table 2. Overview of terms, results section

Parameter Description

ID The assigned ID of a given standard

Well The assigned well

Absorbance The absorbance reading in OD units

The coefficient of variation between repli-
Concentration CV
cates
The entered concentration values for the
Standard conc.
standards
Concentration The calculated concentration values

Mean Absorbance The mean absorbance of replicate wells

19
 Operation

Pos/Neg/Intermediate result of a qualita-

Result*
tive experiment
(*Only appears by qualitative evaluation)

20
 Information required by the user

5 Information required by the user

5.1 Explanation of the signal lights

Table 3. Explanation of the signal lights

Activity of the signal light Meaning

All signal lights flash simultaneously Error, software shows error message
Device is off
No signal lights light up Device is waiting for input
Device is defective
All signal lights light up briefly in sequence Self-test after connection to power
Signal light of the respective measuring
Initialization/measurement in progress
channel flashes/illuminates continuously

5.2 Notes on troubleshooting

If there is an error, all four signal lights flash simultaneously. In this case, check the software
to see which error is present.

In the event of the following errors, a new measurement can be carried out immediately after
the error has been corrected.

Table 4. Error messages and solutions

Restart
Type of error Cause Solution
necessary?
There was a microtiter
Remove the plate/all
plate in the slot during No
objects
initialization.
Initialization failed Clean the inside of the
The inside of the slot slot according to the
No
is dirty cleaning instructions
in chapter 6.2.
Make sure the instru-
ment is not exposed to
Interference due to Too much ambient
too much ambient
ambient light that is light is entering the No
light, e.g. direct sun-
too bright slot
light, during the meas-
urement process.
The ambient temper- Make sure the ambient
Temperature out of
ature was too high or temperature is be- No
specification
too low tween 5-45°C
Use a different USB
USB Power Supply Yes. Remove
port on the computer
Defective (<450mA or Defective USB port cable and re-
or a different com-
< 4V) connect it.
puter

21
 Information required by the user

connect the instru-

ment directly to the
Using a USB hub
computer or use an-
other USB hub.
Defective micro-USB contact service per-
cable sonnel
Irrecoverable hard- Irrecoverable hard- Contact service per-
N/A
ware error* ware error sonnel.
*If the following error occurs, the user cannot correct the error

22
 Maintenance and cleaning

6 Maintenance and cleaning

6.1 Maintenance

The Absorbance 96 is maintenance-free. Each time the instrument is switched on, an internal
self-test is carried out to ensure there are no malfunctions.

There are no parts within the ABSORBANCE 96 that can be serviced by the customer. It is
only necessary to ensure that the device is kept clean.

The accuracy of the device can be checked with a reference plate. If you check more than
one point for accuracy, you can also confirm the linearity of the reader. Byonoy recommends
the reference plate from Hellma GmbH (item code 666-R013), for which the following steps
are described:

1) Measure the plate at the desired wavelength. Ensure that there is no dust or other
contamination on the reference plate.

2) Compare results taking the measurement uncertainty and specifications of the

reader into account and use the following formula: ODRP - MU - SP ≤ ODRES ≤ ODRP +
MU + SP

ODRP: Optical density of the reference plate at the desired wavelength. This value is
found on the calibration certificate from the manufacturer.

MU: Measurement uncertainty of the reference plate. This value is found on the cali-
bration certificate from the manufacturer.

SP: Accuracy specification of the reader at measured optical density. This value is
found in chapter 7.

ODRES: The optical density measured by the reader at the desired wavelength.

Example:

ODRP: 1.554

MU: ± 0.0079

SP: 0.000–2.000 OD ≤ (1 % + 0.010 OD)

ODRES: 1.573

(1)1.554-0.0079-(1.554*0.001+0.010) ≤ ODRES ≤ 1.554+0.0079+(1.554*0.001+0.010)

(2) 1.52056 ≤ ODRES ≤ 1.58744

The measured Optical Density of the reader lies within the calculated range and
hence the accuracy of the reader is verified.

3) Rotate Reference plate by 180 degree and repeat steps 1 and 2.

4) If all Results (ODRES) are within the range, the accuracy and linearity of the device
have been verified.

23
 Maintenance and cleaning

In case that the results are outside the range, please contact the technical service or the
local responsible person.

I Please follow the plate manufacturer’s recalibration instructions for the reference
plate.

6.2 Cleaning

Remove the Micro-USB cable from the instrument before cleaning.

!! Make sure before cleaning, that there is no microtiter plate in the instrument.

Cleaning the housing

The surfaces of the housing should be cleaned regularly. For this purpose, you can use e.g.
a cloth, or a sponge lightly wetted in water. For heavier soiling, clean the surface of the hous-
ing with a mild soap solution diluted with water or glass cleaner and then wipe with a lightly
moistened cloth or sponge to remove any residue. Do not use scouring agents.

Cleaning the slot

To clean the slot of the RIDA ABSORBANCE 96, first open the four screws on the bottom of
the instrument (see Figure 11) using the Hex key provided.

Figure 9. Bottom side of the instrument

I Do not loosen any screws that are not explicitly marked in the manual, as this can
lead to a malfunction and may void the instrument's warranty.

24
 Maintenance and cleaning

Figure 10. Pull the instrument apart into its two components

Carefully pull apart the top and the bottom of the instrument to unplug the plug connection
on the back of the instrument.

I To avoid damaging the rigid plug connection between the top and bottom of the in-
strument, grasp the instrument at the back during the pull movement. Avoid any
tilting movement.

Use a dry microfiber cloth or a bellows to carefully remove dust or dirt from the surface. If
this is not enough, the microfiber cloth can be slightly soaked with 80% ethanol to carefully
remove dust or contamination from the surface.

Do not allow water or other liquids to get inside the instrument. If this happens, return the
device to the manufacturer for inspection.

Warnings for cleaning

When cleaning, pay attention to the following warnings. The non-observance of these warn-
ings may result in damage to the user or to the instrument. This can lead to a reduction of
the service life or in measurement errors:

!! Always avoid spraying liquid directly onto the surfaces of the ABSORBANCE 96.
This is especially important for the inside of the slot, where there are optical ele-
ments that are very sensitive and essential for the functioning of the instrument.

!! Never clean the inside of the slot with sharp, abrasive, solvent-containing agents.
!! Biological hazard: Always wear gloves during cleaning operations that may be asso-
ciated with contact with biological or generally hazardous materials or liquids.

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 Maintenance and cleaning

6.3 Technical support

In case of problems with the instrument, please contact the technical service or the local
responsible person.

!! Biological hazard: It is your responsibility to decontaminate the instrument and all

accessories before servicing and before returning the instrument or accessories to
the manufacturer.

For decontamination of the Absorbance 96, follow the government guidelines for inactiva-
tion of organisms used in biological laboratories.

6.4 Repairs

Repairs on the device may only be carried out by the manufacturer. Please contact the ser-
vice department. The product liability expires if the device is modified by unauthorized per-
sons and by the installation of parts of a different type.

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 Performance data and technical data

7 Performance data and technical data

Table 5. Performance & technical data

Parameter Value
Type of product Absorbance reader
Product name Absorbance 96
Service life 10 years at an average use of 4h/day
Housing material Aluminium
Place for use Laboratory
Degree of contamination 2
Temperature for storage/measurement 5-45°C
Temperature (Transport) -10-50°C
Relative tolerated humidity (storage/measure-
0-70%
ment)
Air pressure (measurement) 900-1070 hPa
Air pressure (storage/transport) 600-1070 hPa
Elevation Max 5100m
Measurement method Absorbance
Measuring technique Endpoint and Kinetic
Microplate types 96-well, fat shaped bottom
Light source 4 LEDs
Filters 4
Detection 96 Photodiodes
Measurement range 0-4.0 OD
Resolution 0.001 OD
0,000–2,000 OD ≤ (1 % + 0,010 OD)
Accuracy*
2,000–3,000 OD ≤ (1,5 % + 0,010 OD)
0,000–2,000 OD ≤ (0,5 % + 0,005 OD)
Reproducibility**
2,000–3,000 OD ≤ (1 % + 0,010 OD)
0,000–2,000 OD ≤ 1 %
Linearity***
2,000–3,000 OD ≤ 1,5 %
Connection to computer USB 2/USB 3 with 5 VDC and max. 3A
Dimensions 55 x 96 x 154 mm
Power supply 5 VDC
Nominal value/Characteristic of the fuse 1A/very fast-acting
Power input 2.5W
Weight 900g

*Accuracy is the maximum deviation between the determined value and the true value;
**Reproducibility is the maximum deviation between the determined values when the
measurement is repeated directly;
***Linearity is the maximum deviation between the true and the determined increase of
the value.

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 Guarantee

8 Guarantee

The warranty period of the Absorbance 96 is 24 months. If a defect occurs in your device
during the warranty period, please contact our service staff directly. The instrument may
only be operated in technically perfect condition.

In the event of defects that could endanger employees or third parties, the device may only
be used again after it has been repaired. This warranty does not cover damage caused by
improper use or external mechanical influences, transport damage or unauthorized inter-
vention in the device by unauthorized persons.

28
 EC-Conformity Declaration

EG-Konformitätserklärung
EC-Conformity Declaration

Byonoy GmbH – Bernstorffstraße 118 - 22767 Hamburg – Germany

Das bezeichnete Produkt entspricht den einschlägigen grundlegenden Anforderungen der

aufgeführten EG-Richtlinien und Normen. Bei einer nicht mit uns abgestimmten Änderung
des Produktes oder einer nicht bestimmungsgemäßen Anwendung verliert diese Erklärung
ihre Gültigkeit.

The Product named below fulfills the relevant fundamental requirements of the EC direc-
tives and standards listed. In the case of unauthorized modifications to the product or an
unintended use this declaration becomes invalid.

Produkttyp
Product type

Absorptionsmessgerät
Absorbance reader

Typenbezeichnung
Type designation

Absorbance 96

Einschlägige EG-Richtlinien/Normen
Relevant EC directives/standards

2011/65/EU; 2014/30/EU;
DIN EN ISO 14971:2013-04; DIN EN 62366-1:2017-07; DIN EN 62304:2016-10; DIN EN
61010-1:2011-07; DIN EN 61010-2-101:2017-10; DIN EN 61326-1:2013-07; DIN EN 61326-2-
6:2013-07

Hamburg, den 08.08.2019

29