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    Exercise 5

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    triciallorin_19
    This document summarizes cultural characteristics of bacteria that can help identify different bacterial species. It discusses how Robert Koch first grew bacteria on solid culture media. It then examines the cultural characteristics of three bacterial species - Pseudomonas aeruginosa, Enterobacter sakazakii, and Micrococcus luteus - by analyzing their growth on different media like KMBA, TZCA, and YDCA. Characteristics observed include colony morphology, turbidity, sedimentation, and pellicle or ring formation.

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    Exercise 5

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    triciallorin_19
    307 views31 pages
    This document summarizes cultural characteristics of bacteria that can help identify different bacterial species. It discusses how Robert Koch first grew bacteria on solid culture media. It then examines the cultural characteristics of three bacterial species - Pseudomonas aeruginosa, Enterobacter sakazakii, and Micrococcus luteus - by analyzing their growth on different media like KMBA, TZCA, and YDCA. Characteristics observed include colony morphology, turbidity, sedimentation, and pellicle or ring formation.

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    This document summarizes cultural characteristics of bacteria that can help identify different bacterial species. It discusses how Robert Koch first grew bacteria on solid culture media. It then examines the cultural characteristics of three bacterial species - Pseudomonas aeruginosa, Enterobacter sakazakii, and Micrococcus luteus - by analyzing their growth on different media like KMBA, TZCA, and YDCA. Characteristics observed include colony morphology, turbidity, sedimentation, and pellicle or ring formation.

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    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    307 views31 pages

    Exercise 5

    Uploaded by

    triciallorin_19
    This document summarizes cultural characteristics of bacteria that can help identify different bacterial species. It discusses how Robert Koch first grew bacteria on solid culture media. It then examines the cultural characteristics of three bacterial species - Pseudomonas aeruginosa, Enterobacter sakazakii, and Micrococcus luteus - by analyzing their growth on different media like KMBA, TZCA, and YDCA. Characteristics observed include colony morphology, turbidity, sedimentation, and pellicle or ring formation.

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    EXERCISE 5: CULTURAL CHARACTERISTICS OF BACTERIA

    BACTERIA
    (bacterium) = relatively simple (Prok.) single-celled; unicellular organisms defined as mcgs considerably smaller than molds (fungi) Antoni van Leeuwenhoek (1676) -first person to see bacteria while studying (pepper) water infusion -reffered to them as wee animalcules

    Wee animalcules: bacteria

    Bacteria
    Robert Koch - first to grow bacteria on solid culture media (gelatin)

    Cultural Characteristics

    help a lot in bacterial identification

    Pseudomonas aeruginosa

    Enterobacter sakazakii

    Micrococcus luteus

    KMBA YDCA TZCA Incubate @28-30; 24-48 hrs then OBSERVE

    NA slant

    TZCA
    unknown

    Incubate @ 28-30 24-48 hrs then


    OBSERVE

    YDCA

    KMBA

    NA

    1: piliform 2: echinulate 3: beaded

    4: effuse 5: aboriscent 6: rhizoid

    punctiform

    granular / spindle

    circular rhizoid

    filamentous

    irregular

    Pellicle, turbidity, sedimentation

    Pellicle, turbidity, sedimentation

    pellicle
    sediments uniform control turbidity

    Pellicle, turbidity, sedimentation


    Pellicle biofilm @ interface bet. air&liquid - polysacch. matrix attached to tube walls containing bacterial cells - community of bacteria - common form of bacterial growth in nature obligate aerobes

    Figure 4. The relative growth requirements for oxygen. The medium without bacteria is yellow, while bacterial growth is indicated by the green color. For example, obligate aerobes and anaerobes, grow respectively only at the top (A) and bottom (D) of a tube of liquid medium. Obligate aerobes often form a film (scum) or pellicle, that floats on the top of the liquid. Facultative aerobes have the best of both worlds, as they are able to grow under both aerobic and anaerobic conditions (C). Microaerophilic bacteria require a little bit of oxygen, but too much is toxic (B).

    Pellicle, turbidity, sedimentation


    Environmental factors that might alter formation of a pellicle: amount of oxygen enzymes

    Pellicle, turbidity, sedimentation


    Turbidity -presence of mcgs. = cloudy
    - cells scatter light passing through
    the suspension

    - cells present, light is scattered - cell mass is proportional to cell


    number

    *motile baceria - also cause turbidity (movement)

    Pellicle, turbidity, sedimentation


    sedimentation -collection of orgs. (settle @ the bottom) obligate anaerobes - grow away from the surface where O2 cannot penetrate

    Pink oxic zone ;Yellow anoxic zone

    A B C D

    aerobic anaerobic facultative microaerophilic E aerotolerant

    KMBA

    Pure culture of P.fluorescens on Kings B medium under normal light B: Pure culture of P.fluorescens on Kings B medium under U.V light

    KMBA

    KMBA

    Pseudomonas aeruginosa
    FORM MARGIN ELEVATION
    Turbidity Sedimentation Pellicle/Ring

    irregular entire umbonate


    ++ +

    TZCA

    Enterobacter sakazakii
    FORM punctiform MARGIN entire ELEVATION convex
    Turbidity Sedimentation Pellicle/Ring + + +

    YDCA

    Micrococcus luteus
    FORM MARGIN ELEVATION
    Turbidity Sedimentation Pellicle/Ring

    circular entire convex


    +; uniform +

    References
    http://www.ispub.com/journal/the-internet-journal-of-veterinary-medicine/volume6-number-1/bio-morphological-characteristics-of-bacterial-species-identifiedfrom-mastitic-milk-samples-of-camel.html http://www.jstor.org/pss/30143814 http://www.aseanfood.info/Articles/11015335.pdf http://bioaliment.ugal.ro/revista/5/Paper%2053.pdf http://www.aseanfood.info/Articles/11015335.pdf http://www.slic2.wsu.edu:82/hurlbert/micro101/pages/Chap4.html Madigan, M.T., J.M. Martinko, D.A. Stahl, D.P.Clark. 2006. Brock Biology of Microorganisms. 13th ed. USA: Benjamin Cummings. pp. 133 and 145.

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