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    Using The Microscope For The Cheek & Onion Cell Lab

    Uploaded by

    Trixie Rose Ebona Cortez
    The document provides instructions for using a microscope, including how to carry, store, clean and identify the basic parts of the microscope. It describes how to properly prepare wet mount slides and observe samples, noting the use of different objectives and focusing techniques. Sample activities are included where students will prepare and observe cheek cells, onion cells, and pond water under the microscope.

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    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd

    Using The Microscope For The Cheek & Onion Cell Lab

    Uploaded by

    Trixie Rose Ebona Cortez
    23 views17 pages
    The document provides instructions for using a microscope, including how to carry, store, clean and identify the basic parts of the microscope. It describes how to properly prepare wet mount slides and observe samples, noting the use of different objectives and focusing techniques. Sample activities are included where students will prepare and observe cheek cells, onion cells, and pond water under the microscope.

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    1.1-wet_mount_preparation-cheek_and_onion_lab(1).pptx

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    The document provides instructions for using a microscope, including how to carry, store, clean and identify the basic parts of the microscope. It describes how to properly prepare wet mount slides and observe samples, noting the use of different objectives and focusing techniques. Sample activities are included where students will prepare and observe cheek cells, onion cells, and pond water under the microscope.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    23 views17 pages

    Using The Microscope For The Cheek & Onion Cell Lab

    Uploaded by

    Trixie Rose Ebona Cortez
    The document provides instructions for using a microscope, including how to carry, store, clean and identify the basic parts of the microscope. It describes how to properly prepare wet mount slides and observe samples, noting the use of different objectives and focusing techniques. Sample activities are included where students will prepare and observe cheek cells, onion cells, and pond water under the microscope.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    of 17

    Using the Microscope for

    the Cheek &


    Onion Cell Lab
    Carrying a Microscope

    A B
    Storing The Microscope

    • Return the lowest power


    objective in place
    • Wrap the cord around th
    e base
    • Return dustcover
    Cleaning the Microscope

    • Use lens paper on all glass parts of the


    microscope.
    • Clean oil immersion lens with chemicals
    provided by your instructor
    Parts of the Microscope
    • Ocular • Fine adjustment knob
    • Body tube • Stage
    • Nosepiece • Stage clips
    • Objective lenses ( • Aperture
    10, 40, 100x)
    • Diaphragm
    • Arm
    • Light
    • Course adjustme
    • Base
    nt knob
    Microscope Parts

    A. Ocular
    B. Body tube
    C. Stage clip
    D. Revolving nose piece
    E. Objective
    F. Arm
    G. Stage
    H. Diaphragm
    I. Lever to move stage clip
    J. Course adjustment
    K. Fine adjustment
    L. Light source
    M. Base
    Using the microscope

    • Always observe using the LOWEST POWER objective f


    irst.
    • Focus using the COARSE ADJUSTMENT KNOB to brin
    g the object into focus. Bring the object into sharp focus
    by using the fine adjustment knob.
    • Focus, and then move to a higher power objective, if ne
    eded.
    • Use only the FINE ADJUSTMENT KNOB when using th
    e HIGHEST (longest) POWER OBJECTIVE.
    • Keep both eyes open to reduce eyestrain.
    • Determine total magnification of the object by multiplyin
    g the power of the ocular (10x) the power by the power
    of the objective.
    Preparing a wet mount
    Start with a clean slide and coverslip.
    Hold the slide and coverslip by their edges to avoid getti
    ng your fingerprints on their surfaces.
    Using tweezers, position the specimen in the centre of t
    he slide.
    With the medicine dropper, place one drop of water on t
    he specimen.
    Hold a coverslip over the sample at a 45° angle. One ed
    ge of the coverslip should touch the surface of the sli
    de near the specimen.
    Lower the opposite edge of the coverslip over the sampl
    e. Make sure that no bubbles form beneath the cover
    slip.
    Animal Cell
    Plant Cell
    Activity
    Prepare wet mount
    • Cheek cell
    • Onion cell

    Look at specimen under high power


    and draw what you see.

    Use proper clean-up technique


    Cheek Cell
    Epidermal Onion Cell
    Activity 3
    Prepare wet mount for dissecting scope
    • Pond water
    Look at specimen under high power and
    draw what you see.
    Use proper clean-up technique
    Dispose Biological material
    Dispose Sharps
    Clean Up

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