Diffusion, Microscope & Cell Lab
Diffusion, Microscope & Cell Lab
Diffusion, Microscope & Cell Lab
Cell Lab
02
Activity 1
Diffusion experiment
Methylene blue MW 320g/mole
Potassium permagnate MW 150 g/mole
Form Hypothesis
T0 initial diameter T60 final diameter
A B
Storing The Microscope
A. Ocular
B. Body tube
C. Stage clip
D. Revolving nose piece
E. Objective
F. Arm
G. Stage
H. Diaphragm
I. Lever to move stage clip
J. Course adjustment
K. Fine adjustment
L. Light source
M. Base
Using the microscope
• Always observe using the LOWEST POWER objective
first.
• Focus using the COARSE ADJUSTMENT KNOB to
bring the object into focus. Bring the object into sharp
focus by using the fine adjustment knob.
• Focus, and then move to a higher power objective, if
needed.
• Use only the FINE ADJUSTMENT KNOB when using
the HIGHEST (longest) POWER OBJECTIVE.
• Keep both eyes open to reduce eyestrain.
• Determine total magnification of the object by
multiplying the power of the ocular (10x) the power by
the power of the objective.
Preparing a slide
• Using a pipet or dropper, add a drop of water or
another solvent to a clean microscope slide.
Then, place the specimen in the water.
• Place the edge of a coverslip on the slide so that
it touches the edge of the water.
• Slowly lower the coverslip to prevent the
formation of air bubbles.
Oil Immersion Lens
40x 100x
Animal Cell
Plant Cell
Activity 2
Prepare wet mount
• Cheek cell
• Elodea cell
• Onion cell