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    Diffusion, Microscope & Cell Lab

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    jydee033
    This document provides instructions for several microscopy activities and experiments. It describes how to set up diffusion experiments with methylene blue and potassium permagnate, how to carry and store a microscope, how to clean microscope parts, identifies microscope parts, and how to properly use a microscope. It also outlines how to prepare slides of cheek cells, Elodea cells, and onion cells for examination under the microscope as well as how to prepare a sample of pond water for examination under a dissecting microscope. The document concludes with a quiz on microscope parts.

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    Attribution Non-Commercial (BY-NC)
    Download as PPT, PDF, TXT or read online from Scribd

    Diffusion, Microscope & Cell Lab

    Uploaded by

    jydee033
    39 views23 pages
    This document provides instructions for several microscopy activities and experiments. It describes how to set up diffusion experiments with methylene blue and potassium permagnate, how to carry and store a microscope, how to clean microscope parts, identifies microscope parts, and how to properly use a microscope. It also outlines how to prepare slides of cheek cells, Elodea cells, and onion cells for examination under the microscope as well as how to prepare a sample of pond water for examination under a dissecting microscope. The document concludes with a quiz on microscope parts.

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    lab 02 microscope

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    This document provides instructions for several microscopy activities and experiments. It describes how to set up diffusion experiments with methylene blue and potassium permagnate, how to carry and store a microscope, how to clean microscope parts, identifies microscope parts, and how to properly use a microscope. It also outlines how to prepare slides of cheek cells, Elodea cells, and onion cells for examination under the microscope as well as how to prepare a sample of pond water for examination under a dissecting microscope. The document concludes with a quiz on microscope parts.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PPT, PDF, TXT or read online from Scribd
    Download as ppt, pdf, or txt
    39 views23 pages

    Diffusion, Microscope & Cell Lab

    Uploaded by

    jydee033
    This document provides instructions for several microscopy activities and experiments. It describes how to set up diffusion experiments with methylene blue and potassium permagnate, how to carry and store a microscope, how to clean microscope parts, identifies microscope parts, and how to properly use a microscope. It also outlines how to prepare slides of cheek cells, Elodea cells, and onion cells for examination under the microscope as well as how to prepare a sample of pond water for examination under a dissecting microscope. The document concludes with a quiz on microscope parts.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PPT, PDF, TXT or read online from Scribd
    Download as ppt, pdf, or txt
    of 23

    Diffusion, Microscope &

    Cell Lab

    02
    Activity 1
    Diffusion experiment
    Methylene blue MW 320g/mole
    Potassium permagnate MW 150 g/mole
    Form Hypothesis
    T0 initial diameter T60 final diameter

    Room temp Fridge


    Carrying a Microscope

    A B
    Storing The Microscope

    • Return the lowest power


    objective in place
    • Wrap the cord around the
    base
    • Return dustcover
    Cleaning the Microscope
     

    • Use lens paper on all glass parts of the


    microscope.
    • Clean oil immersion lens with chemicals
    provided by your instructor
    Parts of the Microscope
    • Ocular • Fine adjustment knob
    • Body tube • Stage
    • Nosepiece • Stage clips
    • Objective lenses • Aperture
    (10, 40, 100x)
    • Diaphragm
    • Arm
    • Light
    • Course
    • Base
    adjustment knob
    Microscope Parts

    A. Ocular
    B. Body tube
    C. Stage clip
    D. Revolving nose piece
    E. Objective
    F. Arm
    G. Stage
    H. Diaphragm
    I. Lever to move stage clip
    J. Course adjustment
    K. Fine adjustment
    L. Light source
    M. Base
    Using the microscope
    • Always observe using the LOWEST POWER objective
    first.
    • Focus using the COARSE ADJUSTMENT KNOB to
    bring the object into focus. Bring the object into sharp
    focus by using the fine adjustment knob.
    • Focus, and then move to a higher power objective, if
    needed.
    • Use only the FINE ADJUSTMENT KNOB when using
    the HIGHEST (longest) POWER OBJECTIVE.
    • Keep both eyes open to reduce eyestrain.
    • Determine total magnification of the object by
    multiplying the power of the ocular (10x) the power by
    the power of the objective.
    Preparing a slide
    • Using a pipet or dropper, add a drop of water or
    another solvent to a clean microscope slide.
    Then, place the specimen in the water.
    • Place the edge of a coverslip on the slide so that
    it touches the edge of the water.
    • Slowly lower the coverslip to prevent the
    formation of air bubbles.
    Oil Immersion Lens

    40x 100x
    Animal Cell
    Plant Cell
    Activity 2
    Prepare wet mount
    • Cheek cell
    • Elodea cell
    • Onion cell

    Look at specimen under high power


    and draw what you see.

    Use proper clean-up technique


    Cheek Cell
    Elodea
    Epidermal Onion Cell
    Activity 3
    Prepare wet mount for dissecting scope
    • Pond water
    Look at specimen under high power and
    draw what you see.
    Use proper clean-up technique
    Dispose Biological material
    Dispose Sharps
    Clean Up
    Microscope Parts Quiz
    Microscope Quiz
    1. Which objective uses oil?
    2. If your ocular is 10x and your
    1.    
    objective is 40x what is the total
    magnification of your image?
    3. Why do you need a cover slip and
    how do you avoid an air bubble?
    4. What is the difference between a
    light microscope and a dissecting
    microscope?
    Instructor’s Notes:
    Materials & Equipment • 5 Rulers
    • 5 dissecting scopes
    • 5 light microscopes • 10 agar plates plus a
    • Cover slips few extra
    • Slides (box)
    • Methylene blue
    • Droppers
    • Water in dropper • Potassium permagnate
    • Oil and cleaner for oil immersion
    • Razor blade to cut
    lens
    plants
    • Lens paper
    • Onion • Pondwater
    • Elodea
    • Use of fridge
    • Toothpicks (box)
    • Sharps and biohazard bag
    • disinfectant

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