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    Microsco PE: Maria Belinda P. Castaneda, MAN, RN Faculty, College of Nursing

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    Maria Jenina Cervantes
    The document defines a microscope and its main parts and functions. It discusses light microscopes, electron microscopes, and atomic force microscopes. Light microscopes are the most common and can magnify up to 1000x, while electron microscopes can magnify over 100,000x allowing visualization of viruses. The document also outlines different types of light microscopes including bright field, dark field, phase contrast, differential interference contrast, fluorescent, and confocal microscopes and their specific uses and capabilities.

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    Microsco PE: Maria Belinda P. Castaneda, MAN, RN Faculty, College of Nursing

    Uploaded by

    Maria Jenina Cervantes
    21 views22 pages
    The document defines a microscope and its main parts and functions. It discusses light microscopes, electron microscopes, and atomic force microscopes. Light microscopes are the most common and can magnify up to 1000x, while electron microscopes can magnify over 100,000x allowing visualization of viruses. The document also outlines different types of light microscopes including bright field, dark field, phase contrast, differential interference contrast, fluorescent, and confocal microscopes and their specific uses and capabilities.

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    3.-microscope-microparv.01

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    The document defines a microscope and its main parts and functions. It discusses light microscopes, electron microscopes, and atomic force microscopes. Light microscopes are the most common and can magnify up to 1000x, while electron microscopes can magnify over 100,000x allowing visualization of viruses. The document also outlines different types of light microscopes including bright field, dark field, phase contrast, differential interference contrast, fluorescent, and confocal microscopes and their specific uses and capabilities.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    21 views22 pages

    Microsco PE: Maria Belinda P. Castaneda, MAN, RN Faculty, College of Nursing

    Uploaded by

    Maria Jenina Cervantes
    The document defines a microscope and its main parts and functions. It discusses light microscopes, electron microscopes, and atomic force microscopes. Light microscopes are the most common and can magnify up to 1000x, while electron microscopes can magnify over 100,000x allowing visualization of viruses. The document also outlines different types of light microscopes including bright field, dark field, phase contrast, differential interference contrast, fluorescent, and confocal microscopes and their specific uses and capabilities.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    of 22

    MICROSCO

    Maria Belinda P. Castaneda, MAN, RN


    Faculty, College of Nursing
    Topic Outline and
    Learning Expectation/s:

    After the topic discussion, learners will be able to:


     Define a Microscope

     Identify its parts and functions

     Familiarize with the different types of

    microscope and its uses


     Recognize some of the micro-organisms seen in

    a microscope
    Definition of
    Terms:
    MICROSCOPE: an optical instrument used to
    magnify micro-organisms a hundredfold or even
    a thousand fold
    Hundredfold: defined as 100 times as much
    Thousand fold: 1,000x
    MICROSCOPY: science of investigating small
    objects and structures using a microscope
    3
    TYPES
     LIGHT microscope – 1,000 x
     ELECTRON microscope –

    100,000x
     ATOMIC FORCE microscope
    LIGHT
    MICROSCOPE
    - often referred to as the OPTICAL microscope, is 
    a type of microscope that commonly uses
    visible light and a system of lenses to magnify
    images of
    small objects
    There are two basic types of optical
    microscopes:
    (1) Simple microscopes: uses the optical power
    of single lens or group of lenses for
    magnification.
    (2) Compound microscope: uses a system of lenses
    A digital microscope is a
    microscope equipped with a
    digital camera allowing
    observation of a sample via
    a computer.
    Digital microscopy allows
    greater analysis of a
    microscope image, for
    example measurements of
    distances and areas and
    quantitaton of a fluorescent
    or histological stain.
    ELECTRON
    MICROSCOPE
    An electron microscope is
    a microscope that uses a
    beam of accelerated
    electrons as a source of
    illumination, and magnets
    to focus the beam

    - higher resolving power


    than light microscopes
    and can reveal the
    structure of smaller
    -Magnifies an object 100,000x or
    more.
    -Used to visualize viruses and the
    sub cellular structures of a cell

    (2) TYPES:
    1. TRANSMISSION ELECTRON
    MICROSCOPE: used to study
    the ultra-structures of cells and
    viruses.
    2. SCANNING ELECTRON
    MICROSCOPE: gives a three-
    dimensional image of the
    object;
    ATOMIC FORCE
    MICROSCOPE
    -a type of high resolution
    scanning probe microscope
    that has a resolution that you
    can measure in fractions of a
    nanometer.
    - allows topographical imaging
    of DNA molecules samples,
    protein adsorption or crystal
    growth, and living cells
    adsorbed on biomaterials
    BRIGHT-FIELD
    MICROSCOPE

     Illuminates FIELD evenly; magnifies 1000 to


    1500x
     For stained & naturally pigmented m.o.
     Use for bacteria & fungi
    DARKFIELD
    MICROSCOPE

     1,000x
     Only the OBJECT is illuminated
     Uses REFLECTED LIGHT instead of
    transmitted light thru OPAQUE DISC
    CONDENSER that blocks light.
    DARKFIELD
    MICROSCOPE
    For examining living micro-organisms that are
    either:
     (1)Invisible under light microscope

     (2)Unable to stain using standard method

    E.g. spirochetes
    PHASE-CONTRAST
    MICROSCOPE
    1,000 x
     Differences in refractive indices & light waves
    passing through transparent objects assume
    different phases.

    Bright Field Phase contrast


    PHASE-CONTRAST
    MICROSCOPE
    PURPOSE
     (1) detailed exam of internal structures

     E.g. Endospores
     (2) study of binary fission & motility
    DIFFERENTIAL INTERFERENCE
    CONTRAST MICROSCOPE
    Nomarski interference contrast
    (NIC)

     HIGHER RESOLUTION
     2 BEAMS OF LIGHT instead of
    one
     Appears 3-dimensional
     COLORS due to prisms
    FLUORESCENT
    MICROSCOPE
    Uses (1) ULTRAVIOLET LIGHT & (2)

    FLUORESCENT DYES called as


    fluorochromes
     Micro-organisms fluoresces against dark field
    FLUORESCENT
    MICROSCOPE
     To detect antigen &
    antibodies
     Ex. Immunofluorescence
    or the Fluorescent –
    antibody technique
    CONFOCAL
    MICROSCOPE
     Micro-organisms is
    stained w/
    FLUORESCENT
    dye to emit light.

     Scanned by LASER
    in PLANES &
    REGION
    CONFOCAL
    MICROSCOPE
     Use COMPUTER
     Produce 3-
    dimensional image
     Use: study physiology
    of cell

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