BIOL 2050 - Lecture 7
BIOL 2050 - Lecture 7
BIOL 2050 - Lecture 7
BIOL 2050
Tarushika Vasanthan, Phd
Chapter: 9
Nov 12th 2021
Lecture 7
RECAP
Blue DNA strands are "heavy" (H) ; Red DNA strands are "light" (L)
All All
1x H : 1x L intermediate intermediate
duplexes duplexes duplexes
Modes of Replication
Chapter 9.2
Modes of Replication
Chapter 9.2
Requirements of Replication
3. Enzymes and other proteins that “read” the template and assemble the substrates into a DNA molecule
Chapter 9.2
Requirements of Replication
Chapter 9.2
Direction of Replication
Chapter 9.2
1. Initiation
2. Unwinding
3. Elongation
4. Termination
Chapter 9.3
Replication: Unwinding
Chapter 9.3
Replication: Elongation
Chapter 9.3
Replication: Elongation
Chapter 9.3
Replication Fork
Each active replication fork requires five basic components:
2. Single-strand-binding proteins to protect the single nucleotide strands and prevent secondary structures
4. Primase to synthesize primers with a 3’-OH group at the beginning of each DNA fragment
5' end
3' end
Chapter 10.1
Classes of RNA
Chapter 10.2
DNA 3’TACACACCT…5’
UGU
RNA 5’
AUGUGUGGA… 3’
Protein
Met- Cys- Gly
are co-linear
GGA
Chapter 10.2
Transcribed Strand
Chapter 10.2
Transcribed Strand
Chapter 10.2
Translation
+ Met
H3N COO- Protein X
Genes can be transcribed (expressed) with different efficiencies
gene C
amount
of RNA
amount
of protein
Chapter 10.2