Scribd Logo
Upload

Welcome to Scribd!

  • 7 views

    4 Incubator

    Uploaded by

    Paban Bhattarai

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd

    4 Incubator

    Uploaded by

    Paban Bhattarai
    7 views10 pages

    Original Title

    4.Incubator

    Copyright

    © © All Rights Reserved

    Available Formats

    PPTX, PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    7 views10 pages

    4 Incubator

    Uploaded by

    Paban Bhattarai

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    of 10

    INCUBATOR

    Table of contents
    1. Introduction
    2. Components of Bacteriological Incubator
    3. Working principle
    4. Significance
    5. Quality Control
    6. Cleaning and maintenance
    7. Reference
    Introduction
    An incubator is an electric equipment used for
    providing favorable environment like temperature,
    humidity , time, and gas required for the growth of
    micro-organism. Incubators are used in various
    applications, including research, medical
    diagnostics, and pharmaceutical development,
    where maintaining precise and consistent conditions
    is crucial for studying bacterial behavior and
    conducting experiments.
    Component of
    Bacteriological
    Incubator
    The instrument works on thermal temperature
    and dry heat mechanism. The set temperature
    is maintained by sensor and cooling system.
    The instrument maintains the physical
    Working environment like temperature, humidity,
    oxygen, and carbon dioxide level as per the
    Principle requirement. They work under the ideal
    temperature range of 35 degree Celsius to 37
    degree Celsius.
    Procedure
    First take out the culture plates from the incubator that are of use.
    Label the petri plates after inoculating bacteria into the growth media.
    Then you can keep the culture plates upside down over the performed racks.
    Afterwards close the door of the incubator. This aids in the growth of bacteria
    within an isolated environment.
    Switch on the main power switch to supply electricity. Set the desired temperature
    depending on the growth conditions of cultured bacteria.
    After sometimes the temperature within the incubator stabilizes .The blower at the
    top circulates the hot air throughout the cabinet.
    Take out the Petri plates and tubes containing the bacteria culture after 24 to 48
    hours.
    Significance
    Bacteriological incubators help in growing bacterial cell cultures.
    Determination of enzymes in the specimen by end point reaction
    method.
    It also helps in maintaining the culture of bacteria for future use.
    Besides, you can perform biochemical tests of the isolated bacteria. It
    helps in determining the type and species of the isolated bacteria.
    Within the incubators, you can grow bacteria at different
    temperatures. It helps in studying the growth pattern of bacteria.
    It helps in drying petri dish.
    The temperature in the incubator
    should be recorded daily. Like all
    Quality laboratory instruments, incubator must
    be cleaned at regular intervals (at least
    control every fort-night) and also after spillage
    of any material, weather infectious or
    non-infectious
    Cleaning and Maintenance

    Clean the incubator regularly Before incubating culture and


    making sure it is test and following overnight
    disconnected from its power culturing of microorganisms,
    supply. check the temperature.

    If the incubator does not have


    temperature display, insert a
    Every three to six months
    thermometer in the vent hole
    (depending on the age and
    through the roof of the
    condition of incubator ),check
    incubator . Adjust the
    the incubator and flex for sigh
    thermostat dial until the
    of weir.
    thermometer shoes the correct
    reading.
    Reference
    • PRAFUL B. GODKAR 3rd
    Edition
    • DARSHAN P. GODKAR 3rd
    Edition
    • MONICA CHEESBROUGH
    • WORLD HEALTH
    ORGANIZATION GENEVA 2nd
    Edition
    • BIOLOGY READER

    You might also like