Authors: Gao, Cuifang | Li, Junjie | Shen, Wanqiang | Yin, Ping
Article Type: Research Article
Abstract: Dynamic Time Warping (DTW algorithm) provides an effective method to obtain the similarity between unequal-sized signals. However, it cannot directly deal with high-dimensional samples such as matrices. Expanding a matrix to one dimensional vector as the input data of DTW will decrease the measure accuracy because of the losing of position information in the matrix. Aiming at this problem, a two-dimensional dynamic time warping algorithm (2D-DTW) is proposed in this paper to directly measure the similarity between matrices. In 2D-DTW algorithm, a three dimensional distance-cuboid is constructed, and its mapped distance matrix is defined by cutting and compressing the distance-cuboid. …By introducing the dynamic programming theory to search the shortest warping path in the mapped matrix, the corresponding shortest distance can be obtained as the expected similarity measure. The experimental results suggest that the performance of 2D-DTW distance is superior to the traditional Euclidean distance and can improve the similarity accuracy between matrices by introducing the warping alignment mechanisms. 2D-DTW algorithm extends the application ranges of traditional DTW and is especially suitable for high-dimensional data. Show more
Keywords: Pattern recognition, similarity distance, dynamic programming, DTW
DOI: 10.3233/IDA-215908
Citation: Intelligent Data Analysis, vol. 26, no. 4, pp. 859-871, 2022
Authors: Yu, Zhui | Ouyang, Jing-Ping | Li, Yin-Ping
Article Type: Research Article
Abstract: Application of glucocorticoids in sepsis or severe infection is disputable in clinic. In this experiment, we studied the effect of dexamethasone on nitric oxide synthases and whether dexamethasone could attenuate endotoxin-induced acute lung injury (ALI). SD rats received 5 mg/kg lipopolisaccharide (LPS) injection. Then arterial oxygen partial pressure (PaO2 ), lung histology, lung tissue nitric oxide (NO) production and expression of nitric oxide synthases (NOS) were detected at 0.5, 1, 2, 3 or 4 h after LPS injection. PaO2 and lung injury deteriorated upon time. Production of NO in lung tissue increased significantly particularly in the first two hours, and …this change was mainly due to the over-expression of inducible NOS (iNOS), but not endothelial NOS (eNOS). Furthermore, a tight positive correlation was observed between lung injury score (LIS) and NO production level in lung tissue. Dexamethasone could ameliorate PaO2 and lung damage evidently, which were paralleled by significant decreases in the production of NO and in the expression of iNOS mRNA. In conclusion, dexamethasone could effectively attenuate endotoxin-induced lung injury through inhibiting iNOS expression and activation in the very early stage of ALI. Show more
Keywords: Acute lung injury, inducible nitric oxide synthases, nitric oxide, dexamethasone
DOI: 10.3233/CH-2009-1162
Citation: Clinical Hemorheology and Microcirculation, vol. 41, no. 2, pp. 117-125, 2009
Authors: Li, Yue-Ying | Liu, Han-Han | Chen, Hong-Lei | Li, Yin-Ping
Article Type: Research Article
Abstract: Objective: To investigate the possibility of adipose-derived mesenchymal stem cells (ADSC) in the treatment of type 1 diabetes (T1D). Methods: ADSC were isolated from the adipotic tissue of abdomen in Sprague–Dawley rats (4–6 week-old,female) and expanded in vitro. Cells were then identified by testing their phenotypes through flow cytometry. Balb/c mice (8 week-old, male) were divided into 3 groups: T1D group, ADSC group and control group. Streptozocin (50 mg/kg·d) were injected intraperitoneally into mice of T1D group and ADSC group for 5 consecutive days to establish the T1D model. In ADSC group, ADSC were injected intravenously on day 3 of …STZ injection. In control group, only PBS was injected. Fasting blood glucose (FGB) level was examined once a week. At the end of the 4th week, animals were killed. The pathological changes of islet were showed by histochemistry through hematoxylin–eosin staining (HE staining). β cell insulin expression was detected by quantum dots immunofluorescence histochemistry. Results: After ADSC administration, FGB levels decreased significantly from the second week. Whereas FGB levels in T1D group increased significantly and continuously during the experimental period. Moreover, ADSC effectively suppressed pancreatic islet damage induced by STZ and increased the expression of insulin protein in pancreatic β cells. Conclusions: Intravenuously injected ADSC can prevent STZ induced β-cell destruction and decrease blood glucose level. Show more
Keywords: Adipose, mesenchymal stem cells, type 1 diabetes, pancreatic β cells
DOI: 10.3233/BME-2012-0694
Citation: Bio-Medical Materials and Engineering, vol. 22, no. 1-3, pp. 97-103, 2012
Authors: Yin, Ping | Wang, Shuang | Wei, Yafen | Wang, Xu | Zhang, Jingdian | Yin, Xiang | Feng, Jiachun | Zhu, Mingqin
Article Type: Research Article
Abstract: Inflammation resolution is regulated by specialized pro-resolving lipid mediators (SPMs) and the levels of SPMs are found decreased in Alzheimer’s disease (AD) brain. We have previously found that one of the SPMs, Maresin1 (MaR1), improved neuronal survival and increase microglial phagocytosis of amyloid-β 1-42 (Aβ42 ); however, the mechanisms underlying the protective mechanism remain further investigation. We aim to investigate the effects of MaR1 on microglial chemotaxis and activation in this study. Both indirect and direct primary neuron and microglia co-culture system was used in this study. Our results showed MaR1 downregulated the increased microglial chemotaxis induced by Aβ42 . …The microglial inactivation marker CD200R was downregulated by Aβ42 and upregulated by MaR1. Pro-inflammatory cytokines secretion such as tumor necrosis factor (TNF)-α were increased by Aβ42 and these changes were revised by MaR1 treatment. In addition, the levels of chemokine monocyte chemoattractant protein (MCP)-1 were increased while the levels of anti-inflammatory factor IL-10 secretion were decreased by Aβ42 , and these changes were abolished by MaR1 treatment. Moreover, by proteomics analysis, we identified cell signaling pathways affected by MaR1 were not only limited to inflammation-related pathways such as P38, but also in pathways involved in cell survival, autophagy, axon formation, and apoptosis, including PI3K/AKT, mTOR, ERK, caspase3, Cdc42, and p75NTR. In conclusion, MaR1 promoted inflammation resolution by inhibiting chemotaxis and regulating activation of microglia. MaR1 played a neuroprotective role by affecting cell signaling pathways involving inflammation, cell survival, autophagy, axon formation, and apoptosis inhibition. Show more
Keywords: Alzheimer’s disease, chemotaxis, Maresin1, microglia, proteomics, resolution of inflammation
DOI: 10.3233/JAD-190682
Citation: Journal of Alzheimer's Disease, vol. 73, no. 2, pp. 503-515, 2020
Authors: Xu, Bi-Qing | Si, Qin | Feng, Yin-Ping | Guo, Jing | Jiang, Li-Ping
Article Type: Research Article
Abstract: BACKGROUND: Patients who have been treated with mechanical ventilation for more than 72 hours are susceptible to symptoms such as hypoxia and respiratory muscle fatigue after weaning, which may result in weaning difficulty and delay, as well as an increased incidence of negative emotions such as anxiety and depression. Correct pulmonary rehabilitation exercise technique and timing can improve the weaning success rate, reduce the disability rate, and reduce the incidence of pulmonary infection, as well as reduce medical expenses. OBJECTIVE: This article provides a review of pulmonary rehabilitation interventions for mechanically ventilated patients, searching relevant literature through databases such as …CNKI and PubMed, aiming to provide guidance for the successful weaning of mechanically ventilated patients. METHODS: We selected articles related to pulmonary rehabilitation interventions for mechanically ventilated patients from CNKI (China National Knowledge Infrastructure) and PubMed over the years. RESULTS: This article provides a comprehensive review of the research on lung rehabilitation for patients who are mechanically ventilated during the weaning process in an effort to serve as a guide for a successful transition from mechanical ventilation. CONCLUSION: Early pulmonary rehabilitation training can effectively increase the pulmonary function level and ventilation function of patients and reduce the duration of mechanical ventilation and hospitalization, and is an effective, safe, and feasible treatment method. Show more
Keywords: Mechanical ventilation, pulmonary rehabilitation, research progress, weaning
DOI: 10.3233/THC-231562
Citation: Technology and Health Care, vol. 32, no. 5, pp. 2859-2864, 2024
Authors: Liu, Lei | Guo, Jing | Ding, Kun | Zhou, Guang-Nao | Feng, Yin-Ping | Zhang, Na-Na
Article Type: Research Article
Abstract: BACKGROUND: Globally, pulmonary tuberculosis is a significant public health and social problem. OBJECTIVE: We investigated the factors influencing the hospitalization cost of patients with pulmonary tuberculosis and grouped cases based on a decision tree model to provide a reference for enhancing the management of diagnosis-related groups (DRGs) of this disease. METHODS: The data on the first page of the medical records of patients with the primary diagnosis of pulmonary tuberculosis were extracted from the designated tuberculosis hospital. The influencing factors of hospitalization cost were determined using the Wilcoxon rank sum test and multiple linear stepwise regression analysis, and the included …cases were grouped using the chi-squared automated interaction test decision tree model, with these influential factors used as classification nodes. In addition, the included cases were grouped according to the ZJ-DRG grouping scheme piloted in Zhejiang Province, and the differences between the two grouping methods were compared. RESULTS: The length of hospital stay, respiratory failure, sex, and age were the determining factors of the hospitalization cost of patients with pulmonary tuberculosis, and these factors were incorporated into the decision tree model to form eight case combinations. The reduction in variance (RIV) using this grouping method was 60.60%, the heterogeneity between groups was high, the coefficients of variance ranged from 0.29 to 0.47, and the intra-group difference was small. The patients were also divided into four groups based on the ZJ-DRG grouping scheme piloted in Zhejiang Province. The RIV using this grouping method was 55.24, the differences between groups were acceptable, the coefficients of variance were 1.00, 0.61, 0.77, and 0.87, respectively, and the intra-group difference was significant. CONCLUSION: When the pulmonary tuberculosis cases were grouped according to the duration of hospital stay, respiratory failure, and age, the results were rather reasonable, providing a reference for DRG management and cost control of this disease. Show more
Keywords: Decision tree model, disease-related diagnostic grouping, hospitalization costs, influencing factors, pulmonary, tuberculosis
DOI: 10.3233/THC-231827
Citation: Technology and Health Care, vol. 32, no. 5, pp. 3139-3152, 2024
Authors: Li, Yin-Ping | Latger-Canard, Véronique | Marchal, Luc | Li, Na | Ou-Yang, Jing-Ping | Stoltz, Jean-François
Article Type: Research Article
Abstract: Immune homeostasis is important for the protection of a host from pathogen aggression, as well as for preventing autoimmunity. Dendritic cells (DCs), the most potent antigen presenting cells, are critical in innate, adaptive immunity and in central tolerance. Recently, their involvement in peripheral tolerance has been shown. Whether DCs induce immunity or tolerance depends on their state of maturation. Different subsets of tolerogenic DCs have been identified in vivo, either in physiological, or pathological conditions, such as tumors, or GVHD. Moreover, tolerogenic DCs can be generated in vitro, by using different culture conditions, such as IL-10 or TGF-β. In our …study, we obtained tolerogenic DCs, by culturing them in the presence of human mesenchymal stem cells (MSCs). Show more
Keywords: Dendritic cells (DCs), tolerogenic DCs, immunity, tolerance, mesenchymal stem cells (MSCs)
Citation: Bio-Medical Materials and Engineering, vol. 16, no. 4, pp. S163-S170, 2006
Authors: Yang, Jing-Wei | Isla, Natalia de | Huselstein, Céline | Sarda-Kolopp, Marie-Nathalie | Li, Na | Li, Yin-Ping | Jing-Ping, Ou-Yang | Stoltz, Jean-François | Eljaafari, Assia
Article Type: Research Article
Abstract: Mesenchymal stem cells (MSCs) have the potential to differentiate into distinct mesenchymal tissue cells. They are easy to expand while maintaining their undifferentiated state, which suggests that these cells could be an attractive cell source for tissue engineering of cartilage. In vitro high density micromass culture has been widely used for chondrogenesis induction. Our objective was to investigate human MSCs cell cycle, viability and differentiation in these conditions. Therefore, to induce human MSCs chondrogenesis, micromasses were cultured in the presence of transforming growth factor-β1 in serum free medium for 21 days. Cell cycle, cell viability and cell phenotype were analyzed …by flow cytometry. From day 0 to 7, the G0/G1 phase increased, whereas the S phase decreased gradually, but cell cycle phases (S, G0/G1 and G2/M) did not significantly change after day 7. Less than 10% of cells were apoptotic, but no necrosis was observed, even at day 21. We observed a decrease in CD90 and CD105 expression, from day 0 to 21. In conclusion, our results demonstrate a good viability of human MSCs in micromass culture during the whole period of culture. Moreover, micromass culture allowed human MSCs to be synchronized at the G0/G1 phase, while their phenotype suggested some degree of differentiation. Show more
Keywords: Mesenchymal stem cell, TGF-β1, viability, cell cycle, differentiation
Citation: Biorheology, vol. 43, no. 3-4, pp. 489-496, 2006
