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    Biochemical Tests

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    lexieacosta4
    Biochemical test (enterics)

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    Descargue como PDF, TXT o lea en línea desde Scribd

    Biochemical Tests

    Cargado por

    lexieacosta4
    2 vistas3 páginas
    Biochemical test (enterics)

    Título original

    BIOCHEMICAL TESTS

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    Biochemical test (enterics)

    Copyright:

    © All Rights Reserved
    Descargue como PDF, TXT o lea en línea desde Scribd
    Descargar como pdf o txt
    2 vistas3 páginas

    Biochemical Tests

    Cargado por

    lexieacosta4
    Biochemical test (enterics)

    Copyright:

    © All Rights Reserved
    Descargue como PDF, TXT o lea en línea desde Scribd
    Descargar como pdf o txt
    de 3

    ENTERICS I

    -True/Primary Intestinal Pathogens- SALMONELLA SEROTYPES DIFFERENTATION


    - Salmonella – Shigella – Yersinia –
    TSI
    BIOCHEMICAL TESTS Salmonella typhi: K/A ; (-)gas ; (+)H2S
    - All are MOTILE except serotype Phillorum and serotype Gallinarum
    - All are (+)GAS except serotype Gallinarum and serotype typhi
    IMVC - all are (+)H2S except S. serotype paratyphi A
    -
    Salmonella: - + - + CITRATE UTILIZATION
    Salmonella typhi: - + - - Postive (+): others
    Shigella: v + - - Negative (-): S. serotype typhi
    Yersinia pestis: - + - - S. serotype paratyphi
    Yersinia enterolitica: v + - -
    (note: only salmonella is CITRATE (+))
    LYSINE DECARBOXYLASE
    TSI • Positive (+)
    Salmonella: K/A ; (+)gas ; (+)H2S S. serotype typhi
    Salmonella typhi: K/A ; (-)gas ; (+)H2S S. serotype choleraesuls
    Shigella • Negative (-)
    Yersinia pestis: K/A ; (-)gas ; (-)H2S S. serotype paratyphi
    Yersinia enterolitica:
    (note: Sh-Y are TSI same) (Shigella flexneri is (+)gas) SUGAR FERMENTATION
    Arabinose (+): S. serotype paratyphi
    UREASE
    Rhamnose (+): S. serotypes paratyphi, choleraesuls
    • Positive (+)
    Trehalose (+): S. serotypes typhi, paratyphi
    Yersinia enteroltica
    Yersinia Pseudotuberculosis
    Escherichia coli
    • Negative (-)
    Shigella sonnei IMVC
    Salmonella (+ + - - )
    Yersinia pestis
    TSI
    SUGAR FERMENTATION A/A ; (+)gas ; (-)H2S
    Mannitol (+): Shigella dysenterae
    Sucrose (+): Yersinia enterolitica Urease
    Lactose: all Yersinia spp are negative Negative (-)
    ORNITIHINE DECARBOXYLASE
    LIA
    • Positive (+)
    Yersinia enterolitica
    K/K ; (-)H2S
    • Negative (-) (-) Lysine deamination
    Salmonella serotype typhi (+) Lysine decarboxylation
    Yersinia pestis
    Yersinia pseudotuberculosis
    ENTERICS II
    -Opportunistic Intestinal Pathogens- PAD(+)
    - KES – PPM – Hafnia – Edwardsiella – Erwinia – Citrobacter - P-P-M group

    BIOCHEMICAL TESTS UREASE


    (v)
    IMVC Enterobacter cloacae
    Klebsiella oxytoca: (+ - + +) Providencia stuartii
    Serratia marcescens (- v + +) Citrobacter freundii, Citrobacter koserii
    Proteus vulgaris: (+ + - v) (++)
    Proteus mirabilis: ( - + v v) Klebsiella pneumoniae, Klebsiella oxytoca
    Hafnia alvei: (- v + -) Proteus spp, Providencia retgerri, Morganella morganii
    Citrobacter freundii: (- + - +) (--)
    (- - + +) Enterobacter aerogens
    Klebsiella pneumoniae: Hafnia alvei
    Enterobacter cloacae Serratia marcenscens
    Enterobacter aerogenes:
    (+ + - +)
    Providencia rettgeri:
    Providencia stuartii:
    Citrobacter koseri:
    (+ + - -)
    Morganella Morganii:
    Edwardsiella tarda

    TSI
    A/A ; (+)gas ; (-)H2S
    E. coli
    Enterobacter
    Klebsiella
    K/A ; (+)gas ; (-)H2S
    Morganella morganii
    Citrobacter koseri
    Serratia mercenscens
    Hafnia alvei
    K/A ; (+)gas ; (+)H2S
    Proteus mirabilis
    Citrobacter freundii
    Edwardsiella tarda
    K/A ; (-)gas ; (-)H2S
    Providencia
    K/A ; (+/-)gas ; (+)H2S
    Proteus vulgaris
    NON-ENTERICS Helicobacter pylori (v)
    - Vibrio – Aeromonas –Plesiomonas - Campylobacter – Helicobacter -
    GROWTH TEMP
    BIOCHEMICAL TESTS 42C= (2-42C) Aeromonas, Campylobacter jejuni, Helicobacter pylori (v)

    TSI
    : A/A ; (-)gas ; (-)H2S
    CITRATE (+)
    Vibrio cholerae
    Aeromonas caviae Vibrio cholerae
    A/A ; (+)gas ; (+)H2S INDOLE (+)
    Aeromonas hyrdophila Vibrio cholerae
    Aeromonas veronii: Vibrio mimicus
    K/A ; (-)gas ; (-)H2S Vibrio vulnificus
    Plesiomonas
    Aeromonas caviae
    OXIDASE Aeromonas hydrophila
    Positive(+) Aeromonas veronii
    Aeromonas spp & Plesiomonas spp CELLOBIOSE (+)
    Campylobacter spp Vibrio vulnificus
    Helicobacter pylori
    -/+
    OTHER TESTS
    Vibrio spp.
    Gelatin liquefaction: Vibrio spp ; Aeromonas spp
    UREASE (+) Hippurate hydrolysis: Campylobacter jejuni
    Helicobacter pylori Indoxyl acetate hydrolysis: Campylobacter jejuni
    Urea Breath test: Helicobacter
    Growth to NaCl PCR: Helicobacter pylori
    0% NaCl: Vibrio spp(+/-) & Plesiomonas(+/-) & Aeromonas
    Metronidazole (susceptible): Helicobacter pylori
    6% NaCl: Vibrio spp
    Cephalothin (30mcg): Campylobacter jejuni (res) ; Helicobacter pylori (sus)
    SUGAR FERMENTATION Nalidixic acid (30mcg): Campylobacter jejuni (sus) ; Helicobacter pylori (res)
    Glucose: Vibrio, Aeromonas, Plesiomonas Vibriostatic 01/09 (150mcg):
    Inositol: Plesiomonas Aeromonas (res) ; Plesiomonas (res) ; Vibrio (sus)
    Mannitol: Vibrio, Aeromonas (+/-)
    Sucrose: Vibrio (+/-), Aeromonas (+/-)

    CATALASE (+)
    Campylobacter jejuni
    Helicobacter pylori

    NITRATE REDUCTION
    Campylobacter jeuni

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