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    Problem Set 3

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    Nigel Kow
    This document contains four problems related to biochemical engineering and chemostat systems. Problem 1 involves determining concentrations in a two-stage chemostat. Problem 2 involves predicting outcomes of competition between two bacteria grown at different dilution rates. Problem 3 involves calculating maximum cell production rate and feed flow rates in a chemostat. Problem 4 involves explaining results from a chemostat study at different feed concentrations using a Monod model.

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    Problem Set 3

    Uploaded by

    Nigel Kow
    223 views2 pages
    This document contains four problems related to biochemical engineering and chemostat systems. Problem 1 involves determining concentrations in a two-stage chemostat. Problem 2 involves predicting outcomes of competition between two bacteria grown at different dilution rates. Problem 3 involves calculating maximum cell production rate and feed flow rates in a chemostat. Problem 4 involves explaining results from a chemostat study at different feed concentrations using a Monod model.

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    Problem set 3 for biochem

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    This document contains four problems related to biochemical engineering and chemostat systems. Problem 1 involves determining concentrations in a two-stage chemostat. Problem 2 involves predicting outcomes of competition between two bacteria grown at different dilution rates. Problem 3 involves calculating maximum cell production rate and feed flow rates in a chemostat. Problem 4 involves explaining results from a chemostat study at different feed concentrations using a Monod model.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    223 views2 pages

    Problem Set 3

    Uploaded by

    Nigel Kow
    This document contains four problems related to biochemical engineering and chemostat systems. Problem 1 involves determining concentrations in a two-stage chemostat. Problem 2 involves predicting outcomes of competition between two bacteria grown at different dilution rates. Problem 3 involves calculating maximum cell production rate and feed flow rates in a chemostat. Problem 4 involves explaining results from a chemostat study at different feed concentrations using a Monod model.

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    of 2

    CN5172 Biochemical Engineering

    Problem Set 3
    3.1

    In a two-stage chemostat system, the volumes of the first and second reactors
    are V1=500 L and V2=300 L, respectively. The first reactor is used for biomass
    production and the second reactor is for a secondary metabolite formation. The
    feed flow rate to the first reactor is F=100 L/h, and the glucose concentration
    in the feed is S=5.0 g/L. Use the following constants for the cells:
    m=0.3 h-1, Ks=0.1 g/L, Yx/S=0.4 g dcw/g glucose
    (a) Determine the cell and glucose concentrations in the effluent of the first
    stage.
    (b) Assume that growth is negligible in the second stage and the specific rate
    of product formation is qp=0.02 g P/g cell-h, and YP/S=0.6 gP/g S.
    Determine the product and substrate concentration in the effluent of the
    second reactor.

    3.2

    Predict the outcomes of the competition between bacterium A and bacterium


    B when grown in a chemostat operated at dilution rates between 0.2h-1 to 1.0h1
    .
    1 .2
    Specific Growth Rate (/h)

    1
    0 .8

    0 .6
    0 .4
    0 .2
    0
    0

    20

    40

    60

    80

    S u b s tra te C o n c . (g /l)

    3.3

    The chemostat growth of a microorganism on glucose gave a cell production


    rate of:
    4 Sx g cells
    and cell yield coefficient of YX/S = 0.1 gg-1.
    qx
    m3h
    3 S 4
    For a chemostat volume of 1m3 and feed glucose concentration of 60 gm-3,
    (a) calculate the feed flow rate for which maximum cell production rate is
    obtained. What is this maximum cell production rate?
    (b) calculate the largest feed flow which can be handled in the given reactor.
    (c) A centrifuge is installed, and a fraction, of the cells is returned to the
    reactor. Determine the recirculation rate, R as a function of if the
    specific growth rate of the cells is the same as that found in (a) is to be
    maintained, and the feed flow rate is 2.5 m3h-1. Hence determine the
    minimum .

    3.4

    A chemostat study was conducted to investigate the effect of the feed limiting
    substrate concentration on the steady state cell concentration. The results are
    graphically represented in Figure 3.4. Explain the results obtained assuming a

    Monod model for cell growth. Comment on the relative magnitude of the
    affinity constant in the cell growth model.
    16
    Feed concentration = 14g/l

    Cell concentration (g/l)

    14

    12

    10

    8
    Feed concentration = 6g/l

    4
    Feed concentration = 2g/l

    0
    0.0

    0.2

    0.4

    0.6

    0.8

    Dilution rate (1/h)

    Figure 3.4

    1.0

    1.2

    Substrate concentration (g/l)

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