G1888-90009 Headspace Sampler

siteprep.
book Page 1 Tuesday, February 17, 2004 9:18 AM
Agilent G1888 Network

Headspace Sampler
Site Prep and Installation

Guide
Agilent Technologies
siteprep.book Page 2 Tuesday, February 17, 2004 9:18 AM
Notices
Agilent Technologies, Inc. 2004
Warranty
No part of this manual may be reproduced in

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into a foreign language) without prior agreement and written consent from Agilent
Technologies, Inc. as governed by United
States and international copyright laws.
The material contained in this document is provided as is, and is subject to being changed, without notice,
in future editions. Further, to the maximum extent permitted by applicable
law, Agilent disclaims all warranties,
either express or implied, with regard
to this manual and any information
contained herein, including but not
limited to the implied warranties of
merchantability and fitness for a particular purpose. Agilent shall not be
liable for errors or for incidental or
consequential damages in connection with the furnishing, use, or performance of this document or of any
information contained herein. Should
Agilent and the user have a separate
written agreement with warranty
terms covering the material in this
document that conflict with these
terms, the warranty terms in the
separate agreement shall control.
Manual Part Number

G1888-90009
Edition
First edition, March 2004
Printed in US
Agilent Technologies, Inc.
2850 Centerville Road
Wilmington, DE 19808-1610 USA
Acknowledgements
Swagelok is a registered trademark of the
Swagelok Company.
Microsoft and Windows are registered
trademarks of the Microsoft Corporation.
Safety Notices
CAU TI O N
A CAUTION notice denotes a hazard. It calls attention to an operating procedure, practice, or the like
that, if not correctly performed or
adhered to, could result in damage
to the product or loss of important
data. Do not proceed beyond a
CAUTION notice until the indicated
conditions are fully understood and
met.
WA RN ING
A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
the like that, if not correctly performed or adhered to, could result
in personal injury or death. Do not
proceed beyond a WARNING
notice until the indicated conditions are fully understood and
met.
Site Preparation and Installation Guide
Manual Overview
This manual contains four chapters and one appendix.
Introduction
This chapter gives an overview of the manual and contains
safety information.
Site Preparation
This chapter provides the requirements for preparing the site
before installing the Headspace Sampler. It covers the
requirements for temperature and humidity, space, power, gas,
and venting.
Installation
Installation describes how to install the Headspace Sampler. It
includes instructions for unpacking the unit, checking the
contents, connecting the power and gas supplies, making
connections to an integrator or Agilent data system, and
connecting the unit to a GC.
Running Checkout Samples

This chapter provides information on preparing and running
the checkout sample to confirm that the sampler is operating
correctly.
Connecting a Swagelok Fitting

This appendix describes proper Swagelok connections.
Contents
1
Introduction
About this Manual 10
Video supplements 10
Important Safety Warnings 11
Many internal parts of the instrument carry dangerous
voltages 11
Electrostatic discharge is a threat to instrument
electronics 11
Many parts are dangerously hot 12
Gases 12
General warnings 13
Safety and Regulatory Certifications 14
Information 14
Symbols 15
Electromagnetic compatibility 15
Sound Emission Certification for Federal Republic of
Germany 16
Fuses
Cleaning
17
17
Recycling the Product

2
17
Site Preparation
Introduction to Site Preparation
20
Temperature and Humidity Requirements

Performance range 21
Optimum performance 21
Site Prep and Installation Guide
21
Space Requirements
22
Power Requirements 23
Line voltage 23
Power line cord 23
Gas Requirements
24
External Plumbing 25
Supply tubing 25
Regulators 25
Venting Requirements
Sampler Considerations
3
28
29
Installation
Installation
32
Unpack the Unit and Check the Contents

Place the Unit in Position
Connect the Power Cord
33
34
35
Connect the Plumbing and Transfer Line 37

Flow modes 37
Typical installation procedures 39
Set the flow mode on the Headspace Sampler
Connect on/off valves to the gas input fittings
MPC connections 42
EPC connections 46
Control Setup 50
Connect the control cables
Set communication options
4
50
52

40
41
56
Prepare the checkout sample 56

Run the sample 57
6850/6890 GC checkout method 58
A

Preparation 70
Objective 70
Materials needed
Procedure
70
71
Agilent G1888 Network Headspace Sampler

1
Introduction
About this Manual 10
Video supplements 10
Important Safety Warnings 11
Many internal parts of the instrument carry dangerous voltages 11
Electrostatic discharge is a threat to instrument electronics 11
Many parts are dangerously hot 12
Gases 12
General warnings 13
Safety and Regulatory Certifications 14
Information 14
Symbols 15
Electromagnetic compatibility 15
Sound Emission Certification for Federal Republic of Germany 16
Fuses 17
Cleaning 17
Recycling the Product 17
This chapter gives an overview of the manual and provides

safety information.
Introduction
About this Manual

This manual explains the requirements and procedures for
installing the Agilent Technologies G1888A Headspace Sampler.
The manual is intended for Agilent Service personnel
performing the installation. It includes basic information for
preparing a site and for installing the Headspace Sampler,
including attaching it to the GC and setting flow rates for
various inlet configurations.
For operating directions, see the Agilent Headspace Sampler
User Information CD ROM.
Video supplements
Click this icon when it appears throughout the manual to view
video supplements to certain procedures.
10
Introduction
Important Safety Warnings

Before moving on, there are several important safety notices
that you should always keep in mind when using the Headspace
Sampler.
Many internal parts of the instrument carry dangerous voltages

If the instrument is connected to a power source, even if the
power switch is off, potentially dangerous voltages exist on:
The wiring between the instrument power cord and the AC
power supply, the AC power supply itself, and the wiring
from the AC power supply to the power switch
With the power switch on, potentially dangerous voltages also
exist on:
All electronics boards in the instrument
The internal wires and cables connected to these boards
The wires for any heater (such as the oven)
WA RN ING
All these parts are shielded by covers. With the covers in place, it
should be difficult to accidentally make contact with dangerous
voltages. Unless specifically instructed to, never remove a cover
unless the heated zones are turned off.
WA RN ING
If the power cord insulation is frayed or worn, the cord must be

replaced. Contact your Agilent service representative.
Electrostatic discharge is a threat to instrument electronics

The printed circuit (PC) boards in the instrument can be
damaged by electrostatic discharge. Do not touch any of the
boards unless it is absolutely necessary. If you must handle
11
Introduction
them, wear a grounded wrist strap and take other antistatic

precautions. Wear a grounded wrist strap any time you must
remove the electronics cover.
Many parts are dangerously hot

Many parts of the instrument operate at temperatures high
enough to cause serious burns. These parts include but are not
limited to:
The carousel and its contents
The sample probe/loop assembly
You should always cool these areas of the instrument to room
temperature before working on them. They will cool faster if you
first set the temperature of the heated zone to room
temperature. Turn the zone off after it has reached the setpoint.
If you must perform maintenance on hot parts, use a wrench
and wear gloves. Whenever possible, cool the part of the
instrument that you will be maintaining before you begin
working on it.
WA RN ING
Be careful when working behind the instrument. During cool-down

cycles, the instrument emits hot exhaust which can cause burns.
Gases
Do not use flammable carrier gases.
Do not use hydrogen as a carrier gas in the Headspace
Sampler. Hydrogen creates a potential explosion hazard due
to the venting of gases during operation and standby.
Wear eye protection when using compressed gas to avoid eye
injury.
Fasten all compressed gas cylinders securely to an
immovable structure or permanent wall.
Store and handle compressed gases in accordance with
relevant safety codes.
Do not put gas cylinders in the path of a hot air vent
(including a GC oven exhaust).
12
Introduction
General warnings
Perform periodic leak checks on supply lines, fittings, and
pneumatic plumbing to prevent a potentially hazardous
condition.
To avoid a potential shock hazard when using liquid solution
to locate leaks, turn the main power switch off and
disconnect the main power cord. Be careful not to spill leak
solution on electrical leads.
13
Introduction
Safety and Regulatory Certifications

The Headspace Sampler conforms to the following safety
standards:
Canadian Standards Association (CSA): C22.2 No. 1010.1
CSA/Nationally Recognized Test Laboratory (NRTL):
UL 61010A1
International Electrotechnical Commission (IEC): 610101
EuroNorm (EN): 610101
The instrument conforms to the following regulations on
Electromagnetic Compatibility (EMC) and Radio Frequency
Interference (RFI):
CISPR 11/EN 55011: Group 1, Class A
IEC/EN 61326
AUS/NZ
This ISM device complies with Canadian ICES-001. Cet appareil
ISM est conforme a la norme NMB001 du Canada.
The instrument is designed and manufactured under a quality

system registered to ISO 9001.
Information
The Agilent Technologies Headspace Sampler meets the
following IEC (International Electro-technical Commission)
classifications: Safety Class I, Transient Overvoltage
Category II, Pollution Degree 2.
This unit has been designed and tested in accordance with
recognized safety standards and is designed for use indoors. If
the instrument is used in a manner not specified by the
manufacturer, the protection provided by the instrument may
be impaired. Whenever the safety protection of the Agilent
Headspace Sampler has been compromised, disconnect the unit
from all power sources and secure the unit against unintended
operation.
14
Introduction
Refer servicing to qualified service personnel. Substituting

parts or performing any unauthorized modification to the
instrument may result in a safety hazard.
Symbols
Warnings in the manual or on the instrument must be observed
during all phases of operation, service, and repair of this
instrument. Failure to comply with these precautions violates
safety standards of design and the intended use of the
instrument. Agilent Technologies assumes no liability for the
customers failure to comply with these requirements.
See accompanying instructions for more

information.
Indicates a hot surface.
Indicates hazardous voltages.
Indicates earth (ground) terminal.
Indicates explosion hazard.
Indicates electrostatic discharge hazard.
Electromagnetic compatibility
This device complies with the requirements of CISPR 11.
Operation is subject to the following two conditions:
1 This device may not cause harmful interference.
2 This device must accept any interference received, including
interference that may cause undesired operation.
15
Introduction
If this equipment does cause harmful interference to radio or

television reception, which can be determined by turning the
equipment off and on, the user is encouraged to try one or more
of the following measures:
1 Relocate the radio or antenna.
2 Move the device away from the radio or television.
3 Plug the device into a different electrical outlet, so that the
device and the radio or television are on separate electrical
circuits.
4 Make sure that all peripheral devices are also certified.
5 Make sure that appropriate cables are used to connect the
device to peripheral equipment.
6 Consult your equipment dealer, Agilent Technologies, or an
experienced technician for assistance.
7 Changes or modifications not expressly approved by Agilent
Technologies could void the users authority to operate the
equipment.
Sound Emission Certification for Federal Republic of Germany

Sound pressure
Sound pressure Lp < 70 dB(A) according to DIN-EN 27779
(Type test).
Schalldruckpegel
Schalldruckpegel LP < 70 dB(A) nach DIN-EN 27779
(Typprufung).
16
Introduction
Fuses
Table 1 lists the fuses required for proper operation. These
fuses should only be accessed by Agilent service personnel.
Table 1
Fuses
Fuse designation
Location
Fuse rating and type
F2, F3
Power line module
10A 250V, glass tube
F3
Terminal near
transformer
10A 250V, glass tube
F3, F4
Power board
6A 125V, glass tube
F1
Power board
1A 250V, glass tube
Cleaning
To clean the unit, disconnect the power and wipe down with a
damp, lint-free cloth.
Recycling the Product

For recycling, contact your local Agilent sales office.
17
Introduction
18
Agilent G1888A Headspace Sampler

Site Preparation and Installation
2
Site Preparation
Introduction to Site Preparation 20
Temperature and Humidity Requirements 21
Performance range 21
Optimum performance 21
Space Requirements 22
Power Requirements 23
Line voltage 23
Power line cord 23
Gas Requirements 24
External Plumbing 25
Supply tubing 25
Regulators 25
Venting Requirements 28
Sampler Considerations 29
This chapter describes requirements and tasks to complete

before installing the Headspace Sampler in the laboratory.
19
Site Preparation
Introduction to Site Preparation

This chapter provides site preparation information for the
Aglient G1888A Headspace Sampler, covering the requirements
for:
Temperature and humidity
Space
Power
Gas
External plumbing
Venting
Contact an Agilent sales representative for the current Site
Preparation Checklist. Use the checklist to ensure that the
proper equipment and parts are present, and that installation is
completed properly. The checklist can also be found on the web
at www.agilent.com/chem. Click Library and search for the
Headspace Sampler model number.
20
Site Preparation
Temperature and Humidity Requirements

Performance range
The Headspace Sampler operates in environment temperatures
from 5 C to 45 C (41 F to 113 F) and 5% to 90% relative
humidity .
The minimum operating temperature for the oven while it is
turned on is 40 C. However, the actual minimum temperature
depends on the environment. Generally, the minimum oven
temperature will equal 20 C above ambient. The oven can be
turned off to use the Headspace Sampler closer to ambient
temperature.
Optimum performance
The optimum temperature and humidity are 20 C to 27 C
(58 F to 80 F) and 50% to 60% relative humidity (without
condensation).
21
Site Preparation
Space Requirements
The Headspace Sampler requires a laboratory bench with an
unobstructed surface approximately 64 cm (25 inches) deep by
46 cm (18.1 inches) wide and capable of supporting at least
30 kg (102 lb.).
The unit is approximately 56 cm (22 inches) high. The area
above it must be clear. Allow enough room to open the top lid of
the Headspace Sampler. Shelves or any overhanging
obstructions limit access to the top of the instrument and
interfere with cooling.
Other devices used with the Headspace Sampler (gas
chromatographs, detectors, integrators, or workstations) will
require additional bench space. See the manual for each
instrument for the applicable space requirements. Figure 1
shows instrument footprints for a sample installation.
PC Monitor
64 cm
Headspace
Sampler
6890 GC
PC Keyboard
46 cm
190 cm
Figure 1
22
Example of space needed on lab bench
Site Preparation
Power Requirements
Line voltage
The Headspace Sampler operates from one of these line voltage
supplies:
115 VAC single phase (90 VAC to 130 VAC), 50 to 60 Hz,
750 VA max
230 VAC single phase (198 VAC to 264 VAC), 50 to 60 Hz,
750 VA max
The Headspace Sampler is shipped with the voltage set to
230 VAC. The maximum power the Headspace Sampler can
accept is 750 VA. The recommended neutral to ground level is a
maximum of 3 VAC. The Headspace Sampler should have a
dedicated ground.
Power line cord

To protect users, the instrument panels and cabinet are
grounded through the three-conductor power line cord in
accordance with International Electrotechnical Commission
(IEC) requirements.
When plugged into a properly grounded receptacle, the
three-conductor power line cord provides power and grounds
the instrument. A properly grounded receptacle has its ground
contact connected to a suitable earth ground. Verify that the
receptacle is properly grounded.
23
Site Preparation
Gas Requirements
The gas entering the Headspace Sampler becomes part of the
carrier gas for the GC column. It may come from a
pressure-regulated purified source (see Figure 3 on page 27) or
from the GC itself. A gas supply connection for vial
pressurization is also required. Gas at 550 kPa (80 psi) is
recommended.
We recommend instrument or chromatographic purity
grades specifically intended for chromatographic use. The gas
purities in Table 2 are recommended for optimal
chromatographic performance.
Table 2
WA RN ING
24
Gas purity requirements
Gas
Purity
Helium
99.9995% pure
Nitrogen
99.9995% pure
Do not use hydrogen in the Headspace Sampler. Hydrogen creates a

potential explosion hazard due to venting during operation and
standby.
Site Preparation
External Plumbing
Supply tubing
The Headspace Sampler ships with plumbing kits for both
electronic and manual pressure control (EPC and MPC,
respectively). The kits contain enough tubing to reach a gas
source 3 meters from the Headspace Sampler. Extra plumbing is
needed if the gas supply is not within 3 meters.
If extra plumbing is needed, use only preconditioned copper
tubing (part number 5180-4196) to supply gases to the
instrument. Ordinary copper tubing contains oils and
contaminants. Plastic tubing is permeable to oxygen and other
contaminants that can damage columns and detectors.
The required tube diameter depends on the distance from the
supply or source to the Headspace Sampler and the total flow
rate for the particular gas. Tubing of 1/8-inch od is adequate
when the supply is less than 3 meters (10 feet) away from the
instrument. Use 1/4-inch od tubing for longer distances.
Gas connection to the Headspace Sampler is made with 1/8-inch
Swagelok bulkhead fittings. Never use Teflon tape on
Swagelok-type fittings.
Do not use pipe dope or liquid thread sealant on any pipe
threads. Use only PTFE plumbing tape for these fittings.
Regulators
Two-stage regulators are recommended. Consult a local gas
supplier for the type and size of cylinder valve connections. If
possible, obtain Compressed Gas Association (CGA) numbers.
Suitable two-stage regulators are also available from Agilent.
If cylinders and regulators are stored outdoors where
temperature fluctuates, use an additional single-stage regulator
at the back of the instrument.
25
Site Preparation
If a single gas source supplies several instruments (via a

manifold or T-connections), the pressure in the lines will
fluctuate. Add single-stage regulators at the back of each
instrument to stabilize the pressure. See Figure 2 below.
Single-stage pressure
regulator
Gas supply
source
Figure 2
550-830 kPa
80-120 psig
410-690 kPa
60-100 psig
Headspace
unit
Single stage regulator used to stabilize pressure
Set regulators to deliver 410 to 690 kPa (60 to 100 psi) to the
instrument. Flow and pressure regulators require at least
140 kPa (20 psi) pressure drop across them to maintain
regulation. See Table 3 for the correct regulator to use with each
outlet fitting.
Table 3
26
CGA Numbers and Pressure Regulator Kit Part Numbers
Cylinder outlet fitting

CGA number
Part number for pressure regulator kit

with 1/8-inch Swagelok outlet
346
5183-4641
540
5183-4643
580
5183-4644
590
5183-4645
Site Preparation
The gas to the Headspace Sampler becomes the carrier gas.

Traps are recommended to protect the columns. See Figure 3.
Two-stage regulator
Gas supply on/off valve
On/Off valve
Moisture trap
Hydrocarbon trap
To Headspace
Sampler
Oxygen trap
Gas supply
Figure 3
Plumbing a pressure-regulated gas purified source
27
Site Preparation
Venting Requirements
During normal operation, some of the vial headspace gas is
vented to the outside of the Headspace Sampler. If the
components of this gas are expected to be toxic or noxious,
place the unit within a fume hood or attach a vent system, at
atmospheric pressure, to the port on the sampler unit. Use a
1/8-inch Swagelok fitting for the connection. See Figure 4 for
the location of the vent line.
Vent line
Figure 4
28
Location of vent line on back of instrument
Site Preparation
Sampler Considerations
One or more optional parts and kits may be required for the
installation, depending on the intended use.
The Headspace Sampler ships with a deactivated flowpath
and a 1-mL sample loop. A 3-mL sample loop (part number
2321700004) can be ordered separately.
To enable software method control of the Headspace Sampler
flows and pressures, an Agilent 6890 or Agilent 6850 GC
must have an auxiliary electronic pressure control (EPC)
module. Order kit G1888-60705. The kit contains the EPC
module, the bleeder weldment, and the interface kit.
To splice the transfer line into the inlet, order the Manual
Pressure Control Interface Kit G1290-60515.
There is an optional wire support for the transfer line for use
on an Agilent 6890 GC. Order Transfer Line Support Kit
G2319-60600.
29
Site Preparation
30

3
Installation
Installation 32
Unpack the Unit and Check the Contents 33
Place the Unit in Position 34
Connect the Power Cord 35
Connect the Plumbing and Transfer Line 37
Flow modes 37
Typical installation procedures 39
Set the flow mode on the Headspace Sampler 40
Connect on/off valves to the gas input fittings 41
MPC connections 42
EPC connections 46
Connect the control cables 50
Set communication options 52
Control Setup 50
This chapter describes steps to install the Headspace Sampler

in the laboratory. It is assumed that the GC has already been
installed, is working properly, and is available for the
Headspace Sampler Installation.
31
Installation
Installation
CAU TI O N
The Headspace Sampler contains metric fittings which are not

compatible with Swagelok or other fittings except for the external gas
connections on the rear of the unit.
To install the Headspace Sampler:

1 Unpack the unit and check the contents. See page 33.
2 Place the unit in a suitable location. See page 34.
3 Connect the power cord. See page 35.
4 Connect the headspace sampler to the gas chromatograph
(GC). See page 37.
5 Connect the control cables to the GC and set communication
options. See page 50.
6 Perform the checkout procedure. See page 56.
Contact an Agilent sales representative for the current
Installation Checklist. Use the checklist to ensure that the
proper equipment and parts are present, and that installation is
completed properly. The checklist can also be found on the web
at www.agilent.com/chem. Click Library and search for the
Headspace Sampler model number.
32
Installation
Unpack the Unit and Check the Contents

Inspect the shipping containers for damage. If a container is
damaged or shows signs of stress, notify both the carrier and
your local Agilent office. Keep all shipping materials for
inspection by the carrier.
Check the items received against the packing lists. If there are
any discrepancies, notify your local Agilent office immediately.
Retain the shipping containers until all contents are checked for
completeness and instrument performance is verified.
33
Installation
Place the Unit in Position

1 Place the Headspace Sampler on a bench top to the left of the
GC.
CAU TI O N
The space above the Headspace Sampler must be free of overhanging

obstructions that could interfere with cooling and limit access to the
top of the instrument or prevent the top from opening.
2 Free the transfer line from the side of the Headspace

Sampler. Open the plastic collar holding the line in place.
Use a small flat blade screwdriver to press the release tab if
necessary. See Figure 5.
Release tab
Figure 5
The transfer line release tab
3 Position the Headspace Sampler so that the transfer line can

easily reach the GC inlet without kinks or sharp bends.
34
Installation
Connect the Power Cord

1 Verify the proper operating voltage setting. The Headspace
Sampler is shipped with the voltage set to 230 VAC. If
needed, set the voltage selector to 115 VAC. Also note the
location of the fuses. See Figure 6.
Voltage selector
Fuses
Figure 6
Power connection
2 Plug the Headspace Sampler power cord into a properly

grounded outlet. See page 23 for power requirements.
35
Installation
3 Turn on the Headspace Sampler to verify that it is working

properly. The Headspace Sampler performs a self-test when
it starts up. Verify that each test is labeled OK. The self-test
information can also be viewed at any time from the
diagnostic screen.
a Press the Menu key.
b Scroll down to Advanced Functions with the cursor keys.
Press Enter.
c Scroll down to Diagnostic and press Enter.
Use the cursor arrows to scroll through the multiple test
screens. A typical diagnostic test screen is shown in Figure 7.
Shutter:
OK
Tray
OK
Carousel
OK
Oven Sensor
OK
Loop Sensor
OK
Figure 7
DIAGNOSTIC
SELF TEST
NOT READY
Self-test screen
See the G1888A Headspace Sampler operating manual for a

description of the system diagnostic messages.
36
Installation
Connect the Plumbing and Transfer Line

Flow modes
The Agilent G1888A Headspace Sampler can operate in two
flow modes:
Electronic pressure control (EPC)
EPC mode requires an EPC inlet and an Auxiliary EPC
control module in the GC. The modules provide carrier and
vial pressurization gas control via GC or Agilent data system
setpoints.
Manual pressure control (MPC)
MPC mode uses the manual flow controller and pressure
regulator on the G1888A Headspace sampler to control the
carrier and vial pressurization gases.
See Figure 8 for the MPC flow controller and pressure regulator
locations.
Flow controller
Pressure regulator
Figure 8
Flow controller and pressure regulator location
37
Installation
There are several ways to connect the Agilent Headspace

Sampler to the GC. The type of connection depends on the GC
model, the selected flow mode, and how the instruments are to
be used. The possibilities include:
EPC connections
Direct connection to a split/splitless inlet The sampler is
inserted between the inlet flow module and the inlet, allowing
real method control, via the GC, of flows and pressures for
consistent results between setups. The system is easy to
duplicate and monitor but requires time to convert back to
normal inlet injection for nonheadspace samples.
Direct connection to a volatiles interface The transfer line from
the sampler is connected to the volatiles interface. See the
6890 GCs user information for instructions.
MPC connections
Needle connection to a split/splitless inlet The transfer line
from the sampler ends in a needle (part no. 2322590005) that is
inserted through the inlet septum. Conversion to normal
injection is rapid and simple.
Needle connection to a cool on-column inlet The transfer line
from the sampler ends in a small od needle (part no.
2322590004) that is inserted through the inlet septum.
38
Installation
Typical installation procedures

For MPC installations with a split/splitless inlet:
1 Verify that the flow mode toggle switches on the Headspace
Sampler are set to MPC (page 40).
2 Connect the on/off valves to the gas input fittings on the
Headspace Sampler (page 41).
3 Connect the gas input fittings to a pressure-regulated gas
source (page 42).
4 Connect the transfer line using a needle connection to a
split/splitless inlet (page 43).
For EPC installations with a split/splitless inlet:

1 Set the flow mode toggle switches on the Headspace Sampler
to EPC (page 40).
2 Connect the on/off valves to the gas input fittings on the
Headspace Sampler (page 41).
3 Connect the vial pressurization gas to the GC auxiliary EPC
module (page 46).
4 Connect the carrier gas and transfer line directly to the
split/splitless inlet by splicing into the carrier gas line
(page 48).
Alternative installations
To install the Headspace Sampler on a 4890D or 5890 Series II
GC, contact Agilent Service.
39
Installation
Set the flow mode on the Headspace Sampler

Choose the flow mode for the carrier gas and vial pressurization
gas and set the flow mode toggle switches on the headspace
sampler.
1 Open the lid of the Headspace Sampler. Locate the two tabs
near the top of the Headspace Sampler on the left and right
sides. Push the tabs towards the back of the instrument and
lift the lid. Use caution when opening the lid due to its
weight. The lid locks in place when fully opened.
2 Set the toggle switches for carrier gas and vial pressurization
gas on the underside of the Headspace Sampler lid to the
necessary positions. See Figure 9. The factory default setting
is MPC.
MPC
EPC
Carrier gas
Vial pressurization gas
Figure 9
40
The pressure control toggle switches
Installation
Connect on/off valves to the gas input fittings

Install two on/off valves and tubing on the gas input fittings as
shown in Figure 10. The figure shows a connection to the MPC
input fittings.
For EPC, use the fittings on the right
For MPC, use the fittings on the left
For EPC
For MPC
Carrier gas in
Vial pressurization gas in
On/Off valves
for leak testing
Figure 10
On/Off valves connected
41
Installation
MPC connections
Carrier gas and vial pressurization gas connections
If both the carrier gas and vial pressurization gas are MPC,
Install a T-fitting to the on/off valves. Run a line from a
pressure-regulated gas source to the T-fitting as shown in
Figure 11.
To pressure
regulated
gas source
Carrier gas in
Vial pressure
T-fitting
On/Off valves
Figure 11
Carrier and vial pressure connections

If only one gas input fitting uses MPC, run a line from a
pressure-regulated gas source directly to the on/off valve
connected to the MPC gas input fitting.
42
Installation
Needle connection from transfer line to a split/splitless inlet

If the Headspace Sampler uses MPC for the carrier gas, the
transfer line connects to the GC inlet by a needle passing
through the inlet septum.
This system easily converts to normal injection. The installation
does not disturb the normal plumbing between the inlet flow
module and the inlet. Normal inlet flow reduces to minimize
dilution of the headspace sample. Most of the flow through the
inlet comes from the sampler.
The Headspace Sampler shipping kit includes a 0.7 mm
outer-diameter (od) transfer line needle (part no. 2322590005).
This needle is also called a transfer line terminal. The 0.7 mm
od needle is most appropriate for use with the purged/packed
inlet and the split/splitless inlet.
A smaller diameter needle (part no. 2322590004) must be used
for on-column injection and may be used with other inlets.
Order this needle separately.
Follow these steps to connect the transfer line to the GC. This
procedure is described for the 6850 GC. Installation is similar
for a 6890 GC.
1 Install a deactivated direct liner (part no. 5181-8818) in the
split/splitless inlet.
2 Remove the standard septum nut from the inlet.
3 Attach the transfer line to the needle. Use two 7-mm
wrenches to tighten it.
4 Insert the needle through the strain relief coupling.
43
Installation
5 Push an 11-mm through-hole septum onto the needle.

Carefully remove any pieces of septum cored by the needle.
Be sure the needle bore is clear. Figure 12 shows the
assembly.
End of transfer line
Top of inlet
Figure 12
44
The needle assembly
Installation
6 Insert the tip of the needle into the inlet. Force the septum (it
is oversized) into the recess in the top of the inlet. See
Figure 13 for the assembled view.
Septum is forced into recess
Figure 13
The transfer line attached to the inlet
7 Screw on and tighten the strain relief coupling.

When operating, set a small flow of carrier gas through the inlet
using the GC inlet control. Set most of the flow using the flow
controller in the Headspace Sampler. Use an electronic flow
meter connected to the inlet split vent to verify the actual flow
rate.
45
Installation
EPC connections
Connect the vial pressurization gas for an EPC setup
An Auxiliary gas channel in the GC supplies vial pressurization
gas. The following steps describe installation for a 6890 GC with
EPC control of headspace. The installation procedure is similar
for a 6850 GC.
1 If the GC does not have an Auxiliary EPC module, install the
G1940A Headspace Interface Kit. Follow the instructions
provided with that kit.
2 Install the external sampler interface kit (part no.
G1888-60705) into the GC. Follow the procedure for
installing onto a back-pressure regulated system.
3 Locate the block on the Auxiliary EPC module that connects
the three gas outlet tubes for the auxiliary channels to the
pneumatics module.
4 Remove the screw that holds the block to the pneumatics
module. Pull the block free of the module and rotate it so that
the frits are on top. See Figure 14.
Frits
Aux 4
Aux 3
Aux 5
Aux 3
Outputs
Aux 4
Aux 5
Figure 14
46
The Auxiliary EPC module gas outlet block
Installation
5 Choose a channel for the vial pressurization control. Remove

that channels frit from the block. Remove the O-ring that
seals the channel.
6 Place an O-ring on a zero-resistance brass tube frit (part no.
G1570-20540). Place the O-ring/frit combination in the block.
7 Reconnect the block to the pneumatics module. Tighten the
screw.
8 Use tubing and Swagelok fittings to connect the appropriate
Auxiliary module output, the bleed weldment, and the VIAL
PRESSURE fitting to the on/off valve on the back of the
Headspace Sampler as shown in Figure 15. Do not disturb the
brass end on the bleed weldment.
Headspace Sampler
Vial pressure fitting
Bleed weldment
Brass end
To Auxiliary module output

GC
Figure 15
Connecting the Headspace Sampler to the auxiliary module
47
Installation
Direct connection from the transfer line to a split/splitless inlet

The GC inlet control channel supplies carrier gas. The following
steps describe installation for a 6890 GC with EPC control of
headspace. The installation procedure is similar on a 6850 GC.
1 Install a split inlet liner (part no. 5183-4647) in the inlet.
2 Locate the carrier line to the split/splitless inlet shown in
Figure 16.
Carrier gas line
Figure 16
The split/splitless inlet carrier gas line
3 Cut the carrier line to the split/splitless inlet about 1 to

2 inches from the inlet body.
a Carefully score the circumference of the tubing.
b Hold the tubing with pliers on each side of the score and
flex it until it breaks free.
c File the edges until they are smooth.
4 Slide the nut onto the inlet side of the carrier line.
48
Installation
5 Attach a zero-dead-volume (ZDV) union to the short piece of

carrier line attached to the inlet as shown in Figure 17.
ZDV union
Carrier line
Ferrule
Nut
Figure 17
Attaching the ZDV union to the carrier line
6 Attach the headspace sampler transfer line to the ZDV union.

See Figure 18.
Transfer line
ZDV union
Figure 18
Connecting the transfer line to the carrier line
7 Use the other ZDV union to connect the free end of the
carrier gas line to the length of tubing provided in the
shipping kit. Route the tubing out the back of the GC to the
rear panel of the sampler.
8 Connect the tubing to the CARRIER fitting on the sampler.
49
Installation
Control Setup
Connect the control cables
Connect the remote start/stop cable to the back of the sampler.
Be sure to connect the spade lug to the ground screw. Connect
the free end of the cable to the GC.
Depending on the setup, other cable connections may be
needed. Refer to Table 4 for an overview of cable connections
used with the Headspace Samplers.
Table 4
Cables for Headspace Samplers
Sampler port
Connect to
Part number
LAN*
Hub*
8121-0940
Remote
6820, 6850 and 6890 GCs
G1530-60930
APG Y-cable
6890N and 5973N
G1530-61200
Serial (RS-232)
PC
G1530-60600
Remote
4890 and 5890 Series II GCs
35900-60700
*Through a network hub or similar connection.
Figure 19 shows some common cabling configurations. These

cabling configurations are valid for the G2070AA and G2922AA
software set.
.
50
Installation
Cables
1.
8121-0940, Cable, 7.5-m

100-BaseT LAN
2.
G1530-60930, 2-m APG

remote cable, 9-pin male
to 9-pin male
3.
G1530-60600, 2-m RS-232

cable, 9-pin female to
9-pin female
1
1
Workstation
Hub or switch
Printer
6890N
1
1
G1530-61200, 2-m
APG Y-cable, two
9-pin male, one
9-pin female
Workstation
Hub or switch
BNC
BNC
4.
G1888A
Local Talk
8121-1013,
USB-DB9 RS-232
adapter cable
Local Talk
5.
Printer
G1888A
5
6850
Workstation
Printer
G1888A
6820
1
1
1
1
Workstation
Hub or switch
Printer
G1888A
Figure 19
5973N
6890N
Common cabling configurations
51
Installation
Set communication options

If using RS-232 or LAN communications, configure the
communications as described below.
LAN settings
If the Headspace Sampler is connected to a LAN, set the IP
Address, subnet mask, and gateway using the Advanced
Functions menu.
1 Press the Menu key.
2 Scroll down to Advanced Functions. Press Enter.
3 Scroll down to LAN Config. and press Enter or
press . followed by 4 on the numeric keypad.
4 The LAN Config. screen is shown in Figure 20. Use the cursor
and keypad to enter new values. Press Enter to set the value.
SET
IP Address
10 .1 .1 .105
Sub Mask
255 .255 .255 .0
Gateway
10 .1 .1 .1
Figure 20
LAN Config.
Running
The LAN Config. screen
After configuring the LAN settings, disable the RS-232 port

using the Advanced Functions menu.
3 Scroll down to Enable RS232 and press Enter or
52
Installation
4 The Enable RS232 screen is shown in Figure 21. Use the

cursor to choose No from the ADV. FUNCT list. Press Enter.
13. Zones Calibration
ADV. FUNCTION
14. LAN Configuration

Yes
15. MAC Address
No
16. Enable RS232
Figure 21
The Enable RS232 screen
Headspace Sampler automatically resets after after disabling

the RS-232 port.
Additional RS-232 settings

If using RS-232 communications with the Headspace Sampler,
enable RS-232 capabilities using the Advanced Functions.
3 Scroll down to Enable RS232 and press Enter or
53
Installation
4 The Enable RS232 screen is shown in Figure 22. Use the

cursor to choose Yes from the ADV. FUNCT list. Press Enter.
13. Zones Calibration
ADV. FUNCTION
14. LAN Configuration

Yes
15. MAC Address
No
16. Enable RS232
Figure 22
54
The Enable RS232 screen

4
Running Checkout Samples 56
Prepare the checkout sample 56
Run the sample 57
6850/6890 GC checkout method 58
This chapter describes the checkout steps for the Headspace

Sampler.
55

Prepare the checkout sample
The checkout sample is three detector-specific compounds in
ethanol. The box contains 1 mL of sample and a bottle of 5-L
capillary pipettes.
The checkout method calls for a 5-L sample in a 20-mL
headspace vial. The default Headspace Sampler vial size is
20 mL. See the Headspace Sampler Operating Manual for
instructions to set the vial size if needed.
1 Snap the top off the checkout sample ampoule. Transfer the
contents to a screw-cap bottle.
2 Dispense one capillary pipette
3 Hold the pipette with clean tweezers. With the checkout
sample bottle and the pipette as close to horizontal as
practical, dip the end of the pipette into the sample. See
Figure 23
1. Grasp
Figure 23
2. Fill
3. Wipe
Filling a pipette
4 The pipette will fill by capillary action. When it has filled

completely, pull the pipette out of the sample. Wipe the
bottom edge of the pipette against the edge of the vial to
remove any droplets on the outside.
5 Drop the filled pipette into a 20-mL headspace vial. Crimp a
cap on the vial.
56
Run the sample

1 Set the method parameters on the GC and Headspace
sampler. See Table 5 on page 58 for checkout settings or load
the stored checkout method as described in 6850/6890 GC
checkout method on page 58. If needed, install the checkout
column and liner.
2 If using MPC, set the carrier gas flow as follows:
a Set the GC inlet split ratio and column head pressure to
the values given in Table 7 on page 61.
b Set the split flow to 4 mL/min. The total flow will read
11 mL/min.
c With an electronic flow meter connected to the GC split
vent, turn up the headspace carrier gas flow until the flow
rate out the split vent is approximately 80 mL/min.
Remove the flow meter. See Figure 24.
NO TE
The GC and Headspace pressure readings should be similar. Record the

headspace pressure readingit can be used to set up future checkout
runs.
57
Electronic flow
meter
Split vent
68
GC50 S
S y e ri e
s te s
m
Figure 24
6850 GC shown
The electronic flow meter connected
3 Run the checkout sample.

The results should be similar to the chromatograms shown on
pages 65 to 68.
6850/6890 GC checkout method

Load the existing checkout method
The Headspace Sampler ships with a stored method containing
the checkout settings. Do the following steps to load the
checkout method.
2 Highlight Advanced Functions with the cursor keys. Press
Enter.
3 Use the cursor keys to select Stored Meth. (Advanced
Function number 7) and press Enter.
58
4 The screen shown in Figure 25 appears. Use the cursor keys

to select Checkout. Press Enter.
DIAGNOSTIC
STORED METH.
Default
Checkout
OQ/PV
READY
MeOH in H2O
Figure 25
The Stored Method screen
Create a new checkout method

If the checkout settings in the stored methods screen are not
acceptable, use Tables 5 through 12 and Figures 26 through 29
to create a new Headspace Sampler checkout method. This
section covers several inlets and detector types. Use the settings
that apply.
Table 5
Headspace Sampler settings
Headspace method
Oven temperature
100 C
Valve temperature
110 C
Transfer line temperature
115 C
Loop volume
1 mL
Vial pressure
15 psi (See Table 11 if using EPC control)
GC cycle time
5.5 min
Vial equib time
7.0 min
Pressurization time
0.08 min
59
Table 5
Headspace Sampler settings (continued)
Headspace method
60
Loop fill time
0.5 min
Loop equib time
0.05 min
Injection time
1.0 min
Table 6
GC oven settings
Oven
Initial temperature
100 C
Initial time
4.00 min
Rate
0 (Off)
Maximum temperature
300 C
Equilibration time
1.00 min
Run time
4.00 min
Table 7
Inlet settings
Inlet (volatiles or split/splitless)
Mode
Split
Initial temperature
250 C (On)
Pressure
19.5 psi (On)
Split ratio
20:1 (G1289)
Split flow
80 mL/min (G1289)
Total flow
87 mL/min (G1289)
Gas saver
Off
Gas type
Helium
Liner
5181-8818
61
Table 8
Column settings
Column
62
Model number
689019091J-413, HP-5 5% Phenyl methyl silicone

685019091Z-413E, HP-1 Methyl silicone
Maximum temperature
325 C
Length
30 m
Diameter
320 m
Film thickness
0.25 m
Mode
Constant flow
Initial flow
4.0 mL/min
Initial pressure
19.5 psi
Average velocity
60 cm/s
Outlet pressure
Ambient
Table 9
Detector settings (FID, NPD, ECD)
Detector
parameters
FID
NPD
Temperature
300 C
300 C
Hydrogen flow
30 mL/min
2.0 mL/min
Air flow
400 mL/min
60 mL/min
Constant makeup
Mode
Constant makeup
Constant
makeup
30 mL/min
Makeup flow
25 mL/min
8 mL/min
Nitrogen or
argon/methane
Makeup gas
type
Nitrogen or helium
Nitrogen or
helium
Flame
On
Electrometer
On
Lit offset
2.0
ECD
On
On
Adjust offset
30
Bead
On
Equilibration
time
63
Table 10
5973 MSD settings
5973 MSD unique parameters

Column
6890/5973A MSD 19091S-433, HP-5MS 5% Phenyl

methyl siloxane
Solvent delay
3 min
Run time
6.5 min
Headspace event time for

GC cycle time
7 min
Scan range
45 to 300
Method used
Atune file
Inlet pressure
12 psi
Column flow
1.1 mL/min with foreline reading of 60
Split ratio
80:1
Injector
External device
Use the settings given in Table 11 when using a GC with EPC

control.
Table 11
GC Aux pressure settings for EPC control
Aux Pressure 3 (for vial pressurization on G1888A)
64
Gas type
Helium
Initial pressure
15 psi (On)
Initial time
0 min
Rate
0 (Off)
Table 12
Checkout sample
Checkout sample
Headspace OQ/PV Standard
Part number
5182-9733
Size
5 L (by micro-pipette) in 20-mL headspace vial
2.037
Type
pA
160
2.476
0.880
FID1 A, (HS_ECD\HSVI0004.D)
Ethanol
Nitrobenzene
(40,000 counts typical)
2.825
140
120
Dichlorobenzene
t-Butyl disulfide
100
80
60
0.858
40
20
0.0
Figure 26
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
min
FID checkout chromatogram
65
2.384
NPD1 B, (HS_NPD\HSNPD002.D)
pA
Nitrobenzene
250
200
150
100
Ethanol
50
0.0
Figure 27
66
0.5
1.0
1.5
2.0
2.5
3.0
3.5
min
NPD checkout chromatogram
ECD1 A, (HS_ECD\HSEC0000.D)
Hz
2.021
Dichlorobenzene
120000
2.461
100000
80000
60000
0.850
2.810
Ethanol
40000
20000
0
0
Figure 28
min
ECD checkout chromatorgram
67
Dichlorobenzene
1000000
TIC: 0201002.D
Abundance
800000
600000
Nitrobenzene
t-Butyl disulfide
400000
200000
0
3.20
3.60
4.00
4.40
4.80
5.20
5.60
6.00
6.40
Time
Figure 29
68
MSD checkout chromatorgram

Site Preparation and Installation
A
Preparation 70
Procedure 71
This chapter describes proper Swagelok connections.
69
Preparation
Objective
To make a leak-free tubing connection that can be taken apart
without damaging the fitting
Materials needed
1/8-inch (or 1/4-inch, if used) preconditioned copper tubing
1/8-inch (or 1/4-inch, if used) Swagelok nuts
Front and back ferrules
Two 7/16-inch (for 1/8-inch nuts) or 9/16-inch (for 1/4-inch
nuts) wrenches
70
Procedure
1 Place a Swagelok nut, back ferrule, and front ferrule to the
tubing as shown in Figure 30.
Front ferrule
Back ferrule
IMPORTANT!
The narrow end of the back
ferrule fits into the rear of
the front ferrule.
Nut
Tubing
Figure 30
Swagelok nuts and ferrules
2 Clamp a stainless steel plug or similar fitting in a bench vise.
CAU TI O N
Do not
Use a separate stainless steel fitting in a vise for initial tightening of

the nut. Do not use an inlet or detector fitting. Strong forces are
required to properly set the ferrules, and damage to an inlet or detector
fitting is very costly to repair.
3 Push the tubing into the stainless steel plug (Figure 31).
4 Make sure that the front ferrule is touching the plug. Slide
the Swagelok nut over the ferrule and thread it onto the plug.
Nut and ferrules
Plug or fitting
held in a vise
Figure 31
Tubing
Assembling the fitting
71
5 Push the tube fully into the plug, then withdraw it

approximately 1 to 2 mm. Finger-tighten the nut (Figure 32).
Nut
Front
ferrule
Insert tubing fully

Back
ferrule
Withdraw tubing 1-2 mm
Tighten nut
Figure 32
Insert the tubing
6 Mark the nut with a pencil line (Figure 33).
Pencil line
Figure 33
Marking the fitting
7 For 1/8-inch Swagelok fittings, use a pair of 7/16-inch

wrenches to tighten the fitting 3/4 of a turn (Figure 34).
For 1/4-inch fittings, use a pair of 9/16-inch wrenches to
tighten them 1-1/4 turn (Figure 34).
72
Figure 34
Final tightening
8 Remove the plug from the fitting. To connect the tubing, with
nut and ferrules, to another fitting, finger-tighten the nut,
then use a wrench to tighten it 3/4 of a turn (1/8-inch
fittings) or 1-1/4 of a turn (1/4-inch fittings).
9 A correctly-swaged connection is shown in Figure 35. Note
that the end of the tubing in a correctly-swaged fitting is not
crushed and does not interfere with the action of the
ferrules.
Figure 35
Completed fitting
73
Agilent Technologies, Inc.

Printed in USA, March 2004

G1888-90009 Headspace Sampler

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What are the requirements for preparing the site before installing the Headspace Sampler?

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What are the steps to properly connect tubing using Swagelok fittings?

What are the steps to properly connect tubing using Swagelok fittings?

siteprep.

book Page 1 Tuesday, February 17, 2004 9:18 AM

Agilent G1888 Network

Site Prep and Installation

siteprep.book Page 2 Tuesday, February 17, 2004 9:18 AM

No part of this manual may be reproduced in

Manual Part Number

Site Preparation and Installation Guide

siteprep.book Page 3 Tuesday, February 17, 2004 9:18 AM

Running Checkout Samples

Connecting a Swagelok Fitting

Site Preparation and Installation Guide

siteprep.book Page 4 Tuesday, February 17, 2004 9:18 AM

Site Preparation and Installation Guide

siteprep.book Page 5 Tuesday, February 17, 2004 9:18 AM

Recycling the Product

Temperature and Humidity Requirements

siteprep.book Page 6 Tuesday, February 17, 2004 9:18 AM

Unpack the Unit and Check the Contents

Connect the Plumbing and Transfer Line 37

Running Checkout Samples

Site Prep and Installation Guide

siteprep.book Page 7 Tuesday, February 17, 2004 9:18 AM

Prepare the checkout sample 56

Connecting a Swagelok Fitting

Site Prep and Installation Guide

siteprep.book Page 8 Tuesday, February 17, 2004 9:18 AM

Site Prep and Installation Guide

siteprep.book Page 9 Tuesday, February 17, 2004 9:18 AM

Agilent G1888 Network Headspace Sampler

This chapter gives an overview of the manual and provides

siteprep.book Page 10 Tuesday, February 17, 2004 9:18 AM

About this Manual

Site Prep and Installation Guide

siteprep.book Page 11 Tuesday, February 17, 2004 9:18 AM

Important Safety Warnings

Many internal parts of the instrument carry dangerous voltages

If the power cord insulation is frayed or worn, the cord must be

Electrostatic discharge is a threat to instrument electronics

Site Prep and Installation Guide

siteprep.book Page 12 Tuesday, February 17, 2004 9:18 AM

them, wear a grounded wrist strap and take other antistatic

Many parts are dangerously hot

Be careful when working behind the instrument. During cool-down

Site Prep and Installation Guide

siteprep.book Page 13 Tuesday, February 17, 2004 9:18 AM

Site Prep and Installation Guide

siteprep.book Page 14 Tuesday, February 17, 2004 9:18 AM

Safety and Regulatory Certifications

The instrument is designed and manufactured under a quality

Site Prep and Installation Guide

siteprep.book Page 15 Tuesday, February 17, 2004 9:18 AM

Refer servicing to qualified service personnel. Substituting

See accompanying instructions for more

Indicates hazardous voltages.

Indicates earth (ground) terminal.