TMOL Ch13 Qas Evens
TMOL Ch13 Qas Evens
TMOL Ch13 Qas Evens
of LIFE
Physical and Chemical Principles
Chapter 13
Specificity of Macromolecular
Recognition
Problems
True/False and Multiple Choice
2. Which of the following is an attribute of the FGFFGFR
family of interactions?
a. Each FGF ligand has high specificity for an FGF
receptor.
b. Because there are 18 FGF and 4 FGFR genes, there
are 72 potential interactions.
c. Signaling specificity is enhanced through
selective expression of only a few receptors per
tissue type.
d. FGFRs are soluble serine/threonine kinases.
e. FGF proteins have highly diverse folds.
4. Most of the binding energy for SH2 domainpeptide
interactions is contributed by:
a.
b.
c.
d.
Aminoaromatic interactions.
The phosphorylation of the peptide tyrosine.
The amino acid in the peptide + 1 position.
The amino acid in the peptide + 3 position.
Quantitative/Essay
(Assume T = 300 K and RT = 2.5 kJmol1 for all questions.)
14. A scientist wants to engineer an antibody to distinguish
between two proteins (Cyclophilin A and Cyclophilin
B) with a specificity of 500 at 1 nM concentration for
each protein. Her starting material is an antibody that
binds with 10 nM KD to both proteins. She finds that
she can easily make mutations that decrease the
affinity for Cyclophilin B without affecting the affinity for
Cyclophilin A. When she achieves the desired specificity,
what is the KD for Cyclophilin B?
Answer:
The Molecules of Life by John Kuriyan, Boyana Konforti, and David Wemmer Garland Science
The Molecules of Life by John Kuriyan, Boyana Konforti, and David Wemmer Garland Science