Ekstrak Paprika
Ekstrak Paprika
Ekstrak Paprika
2. Introduction
Paprika oleoresin as a spice was evaluated by JECFA at its 14th meeting. No ADI was established
because it was recognized that the use of this material was self-limiting for technological and
organoleptic reasons. At its thirty-second Session, the Codex Committee on Food Additives and
Contaminants requested clarification of this evaluation from the Committee. At its 55th meeting,
JEFCA did not evaluate the available data on paprika oleoresin, but reviewed the previous
evaluation of this substance, which stated that oleoresins of paprika ... are derived from a widely
consumed natural foodstuff, and there were no data indicative of a toxic hazard. The use of
oleoresins as a spice was self-limiting and obviates the need for an ADI. The committee
interpreted this statement to mean that the use of paprika oleoresin as a spice is acceptable.
At the 69th meeting, paprika extract was evaluated as a colour, but the assessment was not finalized.
Data is needed on the composition and capsaicin content of batches of paprika extract for use as a
colour produced by a variety of manufacturers. Also, information is needed as to whether the
material used in the toxicological tests submitted was representative of all the products in
commerce. If not, additional toxicological data on representative material would be needed for the
evaluation of paprika extract for use as a colour.
3. Description
The genus Capsicum, which originates from Central and Southern America, belongs to the
Solanaceae family and includes major types of chilli peppers. It includes all the peppers, from the
mildest bell pepper to the hottest habanero. There are five domesticated species: the three most
wide-spread are C. annuum, C. frutescens, C. chinense, together with C. pubescens and C. baccatum
var. pendulum. The first to be introduced worldwide was C. annuum, originating from Mexico. It is
divided into two categories: sweet (or mild) peppers and hot (or chilli) peppers, though modern
plant breeding removed that distinction. At present, C. annuum is the most wide-spread in terms of
household consumption and industrial processing.
Paprika extracts are extensively used in food industry as natural flavouring but also as colouring
agent for many foods such as spicy culinary, meat products, cheese food coatings, popcorn oil and
cheeses. In addition, it is used dried or fresh in various pharmacological preparations. It has a long
history as a source of biologically active compounds, such as flavonoids, phenols, carotenoids,
capsaicinoids and vitamins. Capsicum fruits contain colouring pigments, pungent principles, resins,
protein, cellulose, pentosans, mineral elements and very little volatile oil, while seeds contain fixed
(non-volatile) oil. The fixed oil mainly consists of triglycerides (about 60%) of which linoleic acid
and other unsaturated fatty acids predominate.
Xanthophyll biosynthesis in the pepper fruit involves two biosynthetic pathways, one or the other
dominant depending on the ripeness stage. During ripening, carotenoid chromoplast pigments are
synthesized and esterified to fatty acids. The total or partial esterification of most carotenoids gives
them a high stability against possible thermo- and photo-oxidation reactions.
Paprika preparations are manufactured by solvent extraction from the pepper fruit of the genus
Capsicum annuum, exclusively. There are two types of products prepared:
Oleoresin capsicum: obtained from the longer, moderately pungent Capsicum used in the
production of red pepper. Commercial red pepper oleoresins are mainly used as a spice
supplied in pungency ratings between 80 000 and 500 000 Scoville units (approximately
0,6-3,9 % capsaicin w/w) and a wide range of colour. In the market place practice has
shown that 1 kg of 200 000 Scoville units oleoresin replaces 10 kg of good quality red
pepper.
Paprika extract: obtained from varieties of C. annuum from which paprika powder is
produced. It has a high colour value, but little or no pungency. Commercial paprika
oleoresin is available in different colour strengths up to 150 000 colour units (equivalent to
10.8% total carotenoids). In the market place practice has shown that 1 kg of paprika extract
replaces 12-15 kg of paprika powder with respect to colour intensity.
4. Method of manufacture
4.1. Manufacturing principle
Paprika extract is manufactured by solvent extraction of the dried Capsicum pods. On harvesting,
the pods have a moisture content of up to 90% which has to be reduced to at least 10%. The drying
operation is carried out by sun-drying, in hot air-dryers or in drying chambers.
Prime extract
Paprika oleoresin (which includes paprika and chilli) is manufactured by solvent extraction of the
dried Capsicum pods, followed by solvent removal. Typically, one kg of pods yields 90 to 120 g of
extract.
The pigment concentration in the extract depends mainly on two parameters, the composition of the
fruit and the extraction technique employed. With respect to the fruit, the organic solvent will
extract all of the lipophilic compounds, which are the pigments and the oil from the pepper pericarp.
The oil is present in much higher quantity than the pigments. In addition, the pigments are located
in cellular structures that are more difficult to access for the solvent. The oil is easily extracted at
the early stage of the process and subsequently becomes richer in pigments. At the end of this
process the solvent(s) is/are evaporated.
New extraction methods have been investigated, e.g. fractionation of paprika extract by extraction
with supercritical carbon dioxide. Higher extraction volumes, increasing extraction pressures, and
similarly, the use of co-solvents such as 1% ethanol or acetone resulted in higher pigment yields.
Pigments isolated at lower pressures consisted almost exclusively of -carotene, while pigments
obtained at higher pressures contained a greater proportion of red carotenoids (capsorubin,
capsanthin, zeaxanthin, -cryptoxanthin) and small amounts of -carotene. It is unknown to which
extent these new extraction methods are commercially utilized.
Further processing
The prime extract can undergo a second extraction process with the objective to remove some or all
of the capsaicinoids, which will yield:
(i)
purified capsaicin for commerce and to standardise other Capsicum extracts and
(ii)
a resin which contains less capsaicin than the prime extract and which may be sold as
colour or as a spice oleoresin depending on the analytical results and its flavour
characteristics.
The prime extract, if low in capsaicin, can be subject to further processing to reduce the flavour and
aroma and thus yield a colour extract.
The final extracts are standardized by either adding food-grade oils or turned into water-dispersible
formulations by adding food emulsifiers.
5. Characterization
5.1. Composition of paprika extract
Carotenoids
There has been extensive research into isolation and characterization of the Capsicum colour. in one
study, 34 of 56 the detected carotenoids were identified, including beta-carotene, cryptoxanthin,
violoxanthin, capsanthin and capsorubin. Xanthophylls occur primarily as fatty acid esters in the
pods and oleoresin preparations. Small amounts of free carotene and xanthophyll pigments can also
be found.
The total carotenoid content of the ripe fruits of Capsicum annuum var. lycopersiciforme rubrum
was about 1.3 g/100 g of dry weight, of which capsanthin constituted 37%, zeaxanthin was 8%,
cucurbitaxanthin A was 7%, capsorubin constituted 3.2%, and -carotene accounted for 9%. The
remainder was composed of capsanthin 5,6-epoxide, capsanthin 3,6-epoxide, 5,6-diepikarpoxanthin,
violaxanthin, antheraxanthin, -cryptoxanthin, and several cis isomers and furanoid oxides.
Capsanthin and capsorubin are the major carotenoids in the ripe fruits. Capsanthin can contribute up
to 60% of the total carotenoids. Capsanthin and capsorubin increase proportionally with advanced
stages of ripeness with capsanthin being the more stable of the two. The amount of carotenoids in
fruit tissue depends on factors such as cultivar, maturity stage, and growing conditions.
From the first stages of ripening, the fraction of totally esterified pigments makes up almost 50% of
the total carotenoid content. The proportion of the partially esterified pigment fraction (zeaxanthin
monoester, capsanthin monoester, and capsorubin monoester) in the total carotenoid content
increases, with a gradual decrease in the fraction of free pigments (beta-cryptoxanthin, betacarotene, zeaxanthin, capsanthin, and capsorubin). In the fully ripe stage, a balance is reached
between the three esterification fractions (free, partially esterified, and totally esterified), which
seems to be largely independent of variety.
The highest concentrations of carotenoid esters are found in red chilli (Capsicum frutescens; 17.1
mg/100 g) and orange pepper (Capsicum annuum; 9.2 mg/100 g). Yellow chilli (Capsicum
frutescens), and yellow pepper (Capsicum annuum) contained lesser carotenoid ester amounts (6.8,
3.2, and 2.1 mg/100 g, respectively). Both mono-esters and di-esters have been described with
varying fatty acid components. Capsanthin occurs in the un-esterified form, as mono-ester and as diester whereas capsorubin only occurred as di-ester. cryptoxanthin was only found in un-esterified
form.
Table 1: Content of carotenoids in Capsicum annuum sorted by degree of esterification.
Form
Xanthophyll
Free
-Cryptoxanthin
Cryptoxanthin
Lutein
Violaxanthin
Capsanthin
Neoxanthin
Total
-Cryptoxanthin
Zeaxanthin
Capsanthin
Total
Antheraxanthin
Capsanthin-5,6-epoxid
Capsanthin
Capsorubin
Total
Monoester
Diester
Content
(% of total carotenoids)
6.1
8.5
2.9
2.4
19.9
14.6
3.7
7.6
25.9
11.0
2.1
28.8
12.2
54.1
The composition of paprika extracts manufactured by different methods has been studied. Paprika
extract produced with organic solvents may contain high levels of unsaturated fatty acids. In addition
to fatty acids they also contain high quantities of tocopherols (0.9-1%). The main pigments of the
extracts were identified as esters of lauric, mystiric and palmitic acid. At the end of the extraction
process the solvents are evaporated which causes heat degradation of carotenoids. The extraction of
6. Functional uses
6.1 Technological function
Paprika extract has an extensive use in food industry as a natural colouring agent for foods such as
spicy culinary, meat products, cheese food coatings, popcorn, oil & cheeses. Depending on the
nature of the food (moisture, fat content, texture, background colour) colour saturation is achieved
at the use level of approximately 100 mg/kg (ppm) colouring matter. Increasing the quantity of
paprika extract has no further benefit, neither for the appearance nor for the taste of the food. As a
result, the use of paprika extract as a colour is self-limiting.
6.2 Food categories and use levels
The results of the NATCOL survey of use levels of paprika extract are summarised in Annex I of
this document. Use levels of paprika extract were provided for each food category. Because
different use levels correspond to different colour hues, respondents were asked to provide range of
use levels and a typical value, representing the most common use level. The data provided by
individual paprika extract producers have been combined. Paprika extract producers were asked to
provide use levels standardised to a 100,000 colour units, which corresponds to a total carotenoid
concentration of 7.2%. This factor was used to derive the total carotenoid concentrations in each
foodstuff.
7. Reactions and fate in food
At room temperature and exclusion of light, cis-trans isomerization of the carotenoids easily occurs.
Under light exposure and room temperature, carotenoid structures are broken down, and the
molecules lose all their nutritional, medical and colourant properties. It has been established in model
systems that carotenoids are degraded in a sequence of reactions.
Although the paprika extract is a complex mixture of different carotenoids in oil, the decolouration
reaction can be studied using a simple approach. The whole carotenoid content disappears following
a first order reaction depending on time and temperature. Loss of red and yellow pigments it
temperature-dependent.
Processing of Capsicum annuum into paprika extract involves some operation units where heating is
the driving force to transform the raw material Several studies have been performed to determine
degradation kinetics and the effect of thermal processing in the carotenoid profile present in that
processed product. Some possible degradation products were detected, although their contribution to
the total carotenoid profile was not significant. The high-temperature treatment modified the
carotenoid profile, yielding several degradation products. Taking into account structures of the
identified degradation products, the cyclization of polyolefins could be considered as the general
reaction pathway in thermally induced reactions, yielding also xylene as byproduct and the
corresponding nor-carotenoids.
8. References
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Annex I
Results of NATCOL survey of Paprika Oleoresin usage (Table 3, NATCOL submission, 2007).
Use Levels mg/kg
Oleoresin
Food
Category No.
1.1.2
1.4.4
1.5.2
1.6.2
1.6.2.1
1.6.4.1
1.6.4.2
1.6.5
1.7
2.2.1
2.2.2
2.3
2.4
3.0
4.1.2.3
4.1.2.6
4.1.2.8
4.1.2.8
4.1.2.9
4.1.2.10
4.1.2.11
4.2.2
5.2
5.3
Food Category
Range
Dairy-based
drinks,
flavoured
and/or
fermented (e.g., chocolate milk, cocoa,
eggnog, drinking yoghurt, whey-based drinks)
Cream analogues
Milk and cream powder analogues
Ripened cheese
Ripened cheese, incl. rind
Plain processed cheese
Flavoured processed cheese
Cheese analogues
Dairy-based desserts (e.g., pudding, fruit or
flavoured yoghurt and ice cream)
Fat emulsions containing at least 80% fat
Fat emulsions containing less than 80% fat
Fat emulsions mainly on type oil-in-water,
including mixed and/or flavoured products
based on fat emulsions (excluding desserts
2.4)
Fat-based desserts excluding dairy-based
dessert products of food category 1.7.
Edible ices, including sherbet and sorbet water-based only (excluding dairy based 1.7,
vegetable based 2.7)
Fruit in vinegar, oil, or brine (e.g. pickled
fruits)
Fruit-based spreads (e.g., chutney) excluding
products of food category 4.1.2.5
Fruit preparations, including pulp, purees,
fruit toppings and coconut milk
Jams, jellies, marmalades
Fruit-based desserts, including fruit-flavoured
water-based desserts but excluding edible ice
3.0 and frozen dairy desserts 1.7
Fermented fruit products
Fruit fillings for pastries excluding purees
4.1.2.8
Processed vegetables (including mushrooms
and fungi, roots and tubers, pulses and
legumes, and aloe vera), seaweeds, and nuts
and seeds
Confectionery including hard and soft candy,
nougat, etc. other than food categories 05.1,
05.3 and 05.4
Chewing gum
Typical
Range
Typical
Min
Max
Min
Max
14
183
131.5
1.0
13.2
9.5
61
70
30
61
19
50
50
4
183
490
122
183
144
200
141
490
166
195
111
166
50
75
95
192.5
4.4
5.0
2.2
4.4
1.4
3.6
3.6
0.3
13.2
35.3
8.8
13.2
10.4
14.4
10.2
35.3
12.0
14.0
8.0
12.0
3.6
5.4
6.8
13.9
40
100
50
200
400
400
150
300
250
2.9
7.2
3.6
14.4
28.8
28.8
10.8
21.6
18.0
10
250
150
0.7
18.0
10.8
14
210
57.5
1.0
15.1
4.1
28
630
97.3
2.0
45.4
7.0
28
630
97.3
2.0
45.4
7.0
28
630
100
2.0
45.4
7.2
28
10
630
150
100
100
2.0
0.7
45.4
10.8
7.2
7.2
28
28
630
630
47.5
97.3
2.0
2.0
45.4
45.4
3.4
7.0
45
96
97.3
3.2
6.9
7.0
10
400
150
0.7
28.8
10.8
10
150
100
0.7
10.8
7.2
5.4
6.3
6.4.3
6.5
6.6
7.1.2
7.1.4
7.2
8.3
8.4
9.2
10.4
11.4
12.2.1
12.2.2
12.4
12.5
12.6
12.7
12.9
12.9.1.1
14.1.3.2
14.1.4
15.1
15.2
16.0
Food Category
Range
Typical
Range
Typical
Min
Max
Min
Max
305
811
500
22.0
58.4
36.0
61
1.2
500
1000
400
800
4.4
0.1
36.0
72.0
28.8
57.6
20
490
200
1.4
35.3
14.4
25
1250
200
1.8
90.0
14.4
20
100
200
3780
50
275
1.4
7.2
3.6
19.8
61
520
343
4.4
14.4
272.
2
37.4
20
800
125
1.4
57.6
9.0
0
20
108
2000
50
760
0.0
1.4
3.6
54.7
10
30
200
300
150
150
0.7
2.2
7.8
144.
0
14.4
21.6
100
5
1000
5000
350
205
7.2
0.4
20
10
10
5
300
1000
400
300
115
215
77.5
102.5
60
14
5
14
60
350
20
420
20
100
0.4
1040
200
1000
24.7
10.8
10.8
1.4
0.7
0.7
0.4
72.0
360.
0
21.6
72.0
28.8
21.6
25.2
14.8
8.3
15.5
5.6
7.4
60
97.3
20
97.3
4.3
1.0
0.4
1.0
4.3
25.2
1.4
30.2
4.3
7.0
1.4
7.0
500
150
255
1.4
7.2
0.0
74.9
14.4
72.0
36.0
10.8
18.4