Biotechnology
Biotechnology
Biotechnology
2014-2015
DEPARTMENT
BIOTECHNOLOGY ENGINEERING
VISION
MISSION
PROGRAMME
PROGRAMME
EDUCATIONAL
are:
OBJECTIVES
multi-disciplinary
approach
to
effectively
PROGRAMME
OUTCOMES
Sub
Code
Subject
Scheme of Teaching
Scheme of Examination
Theory/ Practical
Hrs
Credits
Sess.
Univ.
Exam.
Total
BIO 311
Process
Calculations
50
50
100
BIO 312
Microbiology
50
50
100
BIO 362
Microbiology
(Prac.)
50*
00
50
BIO 313
Biochemistry
50
50
100
BIO 363
Biochemistry
(Prac.)
50*
00
50
BIO 314
50
50
100
BIO 364
50*
00
50
AS
306/301
Engineering
Mathematics III
50
50
100
Total
19
28
25
400
250
650
BIO 411
BIO 461
BIO 412
BIO 413
BIO 463
BIO 414
BIO 464
BIO 415
BIO 465
Subject
Molecular
Biology
Molecular
Biology
(Prac.)
Thermodynamics
Chemical
Reaction
Engineering
Chemical
Reaction
Engineering
(Prac.)
Industrial
Biotechnology
Industrial
Biotechnology
(Prac.)
Immunology &
Immunotechnology
Immunology &
Immunotechnology
(Prac.)
Total
Scheme of Teaching
L
Hrs
Credits
Scheme of Examination
Theory/ Practical
Sess.
Univ.
Total
Exam.
50
50
100
50*
00
50
4
4
0
0
0
0
4
4
4
4
50
50
50
50
100
100
50*
00
50
50
50
100
50*
00
50
50
50
100
50*
00
50
20
28
24
450
250
700
Subject
Scheme of Teaching
Scheme of Examination
Theory/ Practical
Credits Sessional Univ. Total
Marks Exam.
4
50
50
100
Hrs
Enzyme
Engineering &
Technology
Enzyme
Engineering &
Technology (Prac.)
Bio-Process
Engineering
Bio-Process
Engineering
(Prac.)
Animal Cell
Culture &
Biotechnology
Animal Cell
Culture &
Biotechnology
(Prac.)
Transport
Phenomena
Transport
Phenomena (Prac.)
50*
00
50
50
50
100
50*
00
50
50
50
100
50*
00
50
50
50
100
50*
00
50
BIO505
Bio-Process
Technology
50
50
100
BIO506
Bioinstrumentation
50
50
100
50
29
550
BIO501
BIO551
BIO502
BIO552
BIO503
BIO553
BIO504
BIO554
BIO507
24
08
32
50
300
850
Subject
BIO601 Recombinant
DNA Technology
BIO651 Recombinant
DNA Technology
(Prac.)
BIO602 Operation
Research
BIO652 Operation
Research (Prac.)
BIO603 Introduction
to
Bio-Informatics
BIO653 Introduction
to
Bio-Informatics
(Prac.)
BIO604 Bioreactor Design
and Operation
BIO605 Down
Stream
Processing
BIO655 Down
Stream
Processing (Prac.)
BIO606 Biomaterials
Total
Scheme of Teaching
Scheme of Examination
Theory/ Practical
Credits Sess.
Univ. Total
Exam.
4
50
50
100
Hrs
50*
00
50
50
50
100
50*
00
50
50
50
100
50*
00
50
50
50
100
50
50
100
50*
00
50
50
50
100
24
32
28
500
300
800
Subject
BIO701
Scheme of Teaching
L
Hrs
50*
00
50
50
50
100
50*
00
50
50
50
100
50
50
100
50*
00
50
50*
00
50
50
00
50
22
450
200
650
BIO705
Environmental
Biotechnology
Environmental
Biotechnology
(Prac.)
Food
Biotechnology
Food
Biotechnology
(Prac.)
Plant Tissue
Culture
Bio-analytical
Techniques
Bio-analytical
Techniques
(Prac.)
Minor Project
(Prac.)
BIO706
BIO751
BIO702
BIO752
BIO703
BIO704
BIO754
Total
Scheme of Examination
Theory/ Practical
Credits Sessional Univ.
Total
Marks
Exam.
4
50
50
100
16
10
semester
26
Sub
Code
Subject
Scheme of Teaching
Scheme of Examination
Theory/ Practical
Hrs
Credits
Sessional
Marks
Univ.
Exam.
Total
BIO801
Major Project
(Prac.)
50*
00
50
BIO802
Enzyme catalyzed
Organic Synthesis
50
50
100
BIO852
Enzyme catalyzed
Organic Synthesis
(Prac.)
50*
00
50
BIO803
Project Management
and Entrepreneurship
50
50
100
BIO804
Modeling and
Simulation of
Bioprocesses
50
50
100
BIO854
Modeling and
Simulation of
Bioprocesses (Prac.)
50*
00
50
Elective-I*
50
50
100
Total
16
10
26
22
350
200
550
Industrial Training
---
---
---
---
22
350
200
550
Total
---
---
---
---
22
350
200
550
BIO805
Option 2
BIO806
Options in Elective - I*
1. Nanobiotechnology
2. Microbial Biodiversity
SYLLABUS
B.E. IN BIOTECHNOLOGY
THIRD SEMESTER
Course Code
Course Title
Type of Course
LT P
Credits
Course Assessment Methods
End Semester Assessment (University Exam.)
Continuous Assessment (Sessional)
Course Prerequisites
Course Objectives
Course Outcome
BIO 311
Process Calculations
Core
400
4
50
50
Fundamental course in physics, chemistry and
mathematics.
1. To develop a fundamental understanding
of the basic principles of process
calculations.
2. To introduce students to calculations
involving material and energy balance for
reaction and separation processes.
3. To learn about the PVT relations and
gaseous mixtures.
4. To introduce students to the use of data
sources for physical and chemical
properties and the estimation of such data.
1. Define and determine properties of
process streams
2. Perform PVT calculations for ideal and
non-ideal gases.
3. Analyze and solve elementary material
balances on single and multiple unit
processes with recycle and bypass for
reactive and non-reactive processes.
4. Perform energy balance calculations on
reactive and non- reactive processes.
5. Perform process calculations using
psychrometric charts and steam tables.
SYLLABUS
Note: The semester question paper of a subject be of 50 Marks having 7 questions of equal marks.
First question, covering the whole syllabus and having questions of conceptual nature, be
compulsory. Rest of the paper will be divided into two parts having three questions each and the
candidate is required to attempt at least two questions from each section.
SECTION-A
Introduction to Engineering Calculations: Units and dimensions, mole concept,
conventions in methods of analysis and measurement, basis, temperature, pressure, the
chemical equations and stoichiometry. Composition of mixtures and solutions: mass
fractions, mole fraction.
Material Balance: Material balance with and without chemical reactions; Material balance
involving multiple subsystems; recycle, bypass and purge. Concept of limiting and excess
reactant, conversion and yield. Metabolic stoichiometry of growth and product formation.
(10)
(15)
SECTION-B
P-V-T relations for gas and gas mixtures, calculations using ideal gas law, vander waals
equation of state. Liquid and liquid mixtures, vapor pressure, saturation, partial saturation
and humidity.
Enthalpy changes, energy balance for simple flow process, calculation of heat capacity,
mean heat capacity, application of the energy balance to systems with and without
reactions. Use of Hesss law for calculation of heats of formation, heats of combustion,
heats of reaction. Kirchoffs equation for calculating heats of reaction at different
temperatures.
(10)
(10)
RECOMMENDED BOOKS
S.
No.
1
NAME
AUTHOR(S)
PUBLISHER
2
3
D.M.Himmelblau,
D.M.Riggs
B.I.Bhatt, S.M.Vora
PHI Learning
Private Limited
Tata McGraw
Hill
Prentice Hall.
Course Code
Course Title
Type of Course
LT P
Credits
Course Assessment Methods
End Semester Assessment (University Exam.)
Continuous Assessment (Sessional)
Course Prerequisites
BIO-312
Microbiology (Theory)
Core
4 0 0
4
50
50
Knowledge of the prokaryotic and eukaryotic
cell structure and the nature and function of
different bio-molecules
1. To familiarize the students with the
Course Objectives
discipline of Microbiology, historical
developments and various extensions of
Microbiology
2. To understand different structural and
functional aspects of microorganisms
3. To understand different methods and
approaches of microbial classification and
techniques
of
working
with
microorganisms
4. To understand the role of microorganisms
in different scenarios
1. The course would enable the students to
Course Outcome
understand the importance and scope of
Microbiology as a discipline.
2. The students would attain knowledge
about the structure, function and diversity
of different microorganisms
3. The students would be empowered to
handle and maintain microbial cultures
and understand different microbial
interactions at various levels
4. The course would enable them to realize
the scope to engineer microorganisms for
greater use
SYLLABUS
Note: The Semester question paper of a subject be of 50 Marks having 7 questions of equal marks.
First question, covering the whole syllabus and having questions of conceptual nature, be
compulsory. Rest of the paper will be divided into two parts having three questions each and the
candidate is required to attempt at least two questions from each section.
SECTION-A
Brief history of microbiology. Scope and application of microbiology to (3)
biotechnology (in agriculture, food, health, environment, industry)
Microbial diversity- Fine structure of bacteria, fungi, algae, virus
(8)
(9)
(4)
(6)
(5)
(6)
(4)
PUBLISHER
Tata McGraw Hill
(2003) 5th ed.
2.
General
Microbiology
3.
Benjamin Cummings
(2009)10th ed.
4.
Brock Biology of
Microorganisms
Benjamin Cummings
(2010) 13th ed.
5.
Prescott, Harley
and Kleins
Microbiology
Course Code
Course Title
Type of Course
LT P
Credits
Course Assessment Methods
End Semester Assessment (University Exam.)
Continuous Assessment (Sessional)
Course Prerequisites
Course Objectives
BIO 362
Microbiology (Practical)
Core
003
2
00
50
Microbiology (Theory)
1. To impart to students skills to work with
microorganisms and familiarize them with
different techniques used in a microbiology
lab
1. The students learn to isolate, grow, identify
Course Outcome
and maintain different microbial cultures
2. Become proficient to use microbial
techniques in different scenarios pertaining
to biotechnology
List of Experiments
1. To prepare different culture media-broth and agar
2. To learn the culturing of microorganisms by simple streaking and pure culture technique
3. To learn the preservation of microorganisms on agar slants
4. To isolate microorganisms from soil/ milk/food sample by dilution plate method.
5. To study the working of a compound microscope
6. To perform Gram staining of a given microbial sample
7. To perform endospore staining of endospore forming microorganisms
8. To perform motility test on the given bacterial sample
Course Code
Course Title
Type of Course
LT P
Credits
Course Assessment Methods
End Semester Assessment (University Exam.)
Continuous Assessment (Sessional)
Course Prerequisites
Course Objectives
Course Outcome
BIO 313
Biochemistry (Theory)
Core
400
4
50
50
Knowledge of cell structure and functions of
different cell types, organ system. Knowledge of
mammalian, plant and microbial systems in
Biotechnology.
1. To study the structures and functions of
various biomolecules like carbohydrates,
lipids, nucleic acids, proteins and vitamins in
biosystem.
2. To
understand
the
structure-function
relationship in proteins, purification and
sequencing of proteins/enzymes.
3. To study the anabolic and catabolic pathways
of carbohydrates and fats and its significance.
4. To understand the biochemical pathways of
nucleic acid biosynthesis and degradation.
5. To study the biochemical pathways of
photosynthesis and nitrogen fixation in plants
and microbes respectively.
1. Students learn the basic concepts of
biochemistry such as structural and functional
aspects of biomolecules, necessary for
biotechnology studies and applications.
2. Students understand how biomolecules
function as a unit to build a complex
multicellular organism, undergoing diverse
functions and cellular metabolic pathways in
mammals, plants and microorganisms.
3. Students learn the role of enzymes and other
molecules in metabolic pathways and how
regulation of biochemical pathways is attained
in a cell.
SYLLABUS
Note: The Semester question paper will be of 50 Marks having 7 questions of equal marks. First
question, covering the whole syllabus and having questions of conceptual nature, be compulsory.
Rest of the paper will be divided into two parts having three questions each and the candidate is
required to attempt at least two questions from each section.
SECTION-A
NAME
AUTHOR(S)
PUBLISHER
Lehningers Principles of
Biochemistry
Harpers Biochemistry
Biochemistry
Biochemistry
Stryer, Lubert
Biochemistry: clinical
correlations
Outlines of biochemistry
Zubay, GL
4
5
5
4
5
3
4
Course Code
Course Title
Type of Course
LT P
Credits
Course Assessment Methods
End Semester Assessment (University Exam.)
Continuous Assessment (Sessional)
Course Prerequisites
Course Objectives
Course Outcome
BIO 363
Biochemistry (Practical)
Core
003
2
00
50
Understanding
of
biomolecules
and
biochemistry.
1. To give practical training of the analysis
of various biochemical parameters having
clinical and industrial relevance.
2. To have an understanding of the principle
of each determination by biochemical
reactions using UV visible spectroscopy.
3. To
resolve
biomolecules
by
chromatographic technique.
1. Students have hands on training of
quantitations related to biomolecules in
biochemistry.
2. Students learn quality control in clinical
biochemistry by performing blood
analysis.
SYLLABUS
List of Experiments:
1. To study Beer-Lambert Law and to determine max of a colored dye.
2. To estimate carbohydrate content in a given sample by Anthrone method.
3. To determine total amount of proteins in serum by Biuret method.
4. To estimate proteins by Lowry method.
5. To identify the amino acids in a mixture by ascending paper chromatography.
6. To determine the content of nucleic acids by UV method.
7. To quantitate RNA/DNA in the given sample by colorimetric method.
8. To determine cholesterol/ urea/uric acid in blood.
Course Code
Course Title
BIO 314
Cell Biology and Genetics (Theory)
Type of Course
LT P
Credits
Course Assessment Methods
End Semester Assessment (University Exam.)
Continuous Assessment (Sessional)
Course Prerequisites
Core
400
4
Course Objectives
Course Outcome
50
50
Knowledge of eukaryotic cell structure along
with some genetics concepts like about DNA
and chromosomes
1. The topics in this subject would help
students in understanding various
characteristics of cell that are important in
cell behavior.
2. The emphasis has been laid to provide
students with cells architectural as well as
structure assortment that are important to
its normal functions.
3. The information regarding how cells
transfers its characters to next generation
have been comprehensively discussed and
elaborated in topics covering cell genetics.
4. It is hoped that students understanding
about various intricacies of cell, structure
and function would be sorted out.
1. Student would be able to understand about
various characteristics of cell that are
important in cell behavior
2. Student would be able to understand
various intricacies of cell, its structure,
function
3. The course also enable them to understand
that how cell transfers its characters to
next generation
SYLLABUS
Note: The Semester question paper of a subject be of 50 Marks having 7 questions of equal marks.
First question, covering the whole syllabus and having questions of conceptual nature, be
compulsory. Rest of the paper will be divided into two parts having three questions each and the
candidate is required to attempt at least two questions from each section.
SECTION-A
SECTION-B
Molecular genetics: C value paradox, cot curve, transposons (bacterial, eukaryotic,
retrotransposons, viral), gene families, homologus gene, pseudogene
Extensions of Mendelian principles : Codominance, incomplete dominance, gene
interactions, pleiotropy, genomic imprinting, penetrance and expressivity, phenocopy
Chromosome Theory of Inheritance The chromosome theory of heredity, Sex
Chromosomes and sex determination, Genetic linkage and Genetic mapping, sex linkage,
sex limited and sex influenced characters
Extra chromosomal inheritance: Inheritance of Mitochondrial and chloroplast genes,
maternal inheritance
Cytogenetics: Human Karyotype, chromosome banding, ploidy, chromosome aberrations
(6)
(3)
(3)
(4)
(1)
(2)
(3)
(4)
(5)
(7)
(2)
(5)
RECOMMENDED BOOKS
S. No.
1
NAME
Molecular Cell Biology
4
5
6
AUTHOR(S)
Lodish HF, Baltimore D, Berk
A, Zipursky L, P.
Matsudaira P, and Darnell JE
Albert B, Johnson A, Lewis J,
Raff M, Roberts K, Walter P
Cooper GM, Hausman RE,
Karp G
Gardner EJ, Simmons MJ,
Snustad DP
Hartl DL& Jones EW
PUBLISHER
Wiley & Sons (2003)
4th edition
Garland Science,
Taylor and Francis
group (2008) 5th edition
ASM Press (2007) 4th
edition
John Wiley & Sons, 6th
edition
John Wiley & Sons
(1991) 8th edition
Jones and
Bartlett Publication,
(2008) 6th edition
Genetics
Strickberger
Genetics
Course Code
BIO 364
Course Title
Type of Course
LT P
Credits
Core
002
1
00
50
Cell Biology and Genetics (Theory)
Course Objectives
Course Outcome
List of Experiments:
1. Observation of cell cycle and cell division related permanent slides
2. Determination of bacterial cell density by counting of bacterial cells using hemocytometer
3. Determination of cell no. (viable/nonviable) in bacterial cell population
4. To study structure of cell from onion leaf peels
5. Extraction of collagen ECM using glacial acetic acid
6. Chromosomal preparation of mitotic cell division using onion root tip and observation
under simple microscope
7. Staining of DNA and RNA using methyl green and pyronin stains
8. Identification of Barr Body in human Buccal smear
Course Code
Course Title
AS -301
Engineering Mathematics III (Theory)
Type of Course
LT P
Credits
Course Assessment Methods
End Semester Assessment (University Exam.)
Continuous Assessment (Sessional)
Core
310
4
Course Prerequisites
Course Objectives
50
50
Differential and integral calculus of one and
more than one variable (as studied in
Engineering Mathematics I), basic
knowledge of matrices, operations on
matrices, number system and fundamentals of
complex numbers (as studied in senior
secondary classes).
This course is designed to provide an
introduction to Sequences and Series, Linear
algebra and Complex functions. Particularly,
this course will:
1. Present basic concepts of sequences and
series, namely limit of sequence,
convergence and divergence of an infinite
series, error estimates.
2. Present an introduction to the fundamental
concepts of Linear algebra, namely linear
dependence and independence of vectors,
rank of matrix, solution of a system of
linear equations by Gauss elimination
method and inverse of a matrix by GaussJordan elimination method, eigen value
problem, Cayley-Hamilton
theorem,
similarity of matrices etc.
3. Present an introduction to the basic
concepts of complex functions, namely
continuity, differentiability of complex
functions, analytic function, CauchyRiemann equations, Taylor and Laurent
series, concept of residue, conformal
mappings
and
linear
fractional
transformations etc.
Course Outcome
SYLLABUS
Note: The Semester question paper of a subject be of 50 Marks having 7 questions of equal marks.
First question, covering the whole syllabus and having questions of conceptual nature, be
compulsory. Rest of the paper will be divided into two parts having three questions each and the
candidate is required to attempt at least two questions from each section.
SECTION-A
Sequences and Series: Sequences, Limits of sequences, Infinite series, series of (8)
positive terms, Integral test, Comparison test, Ratio test, Root test. Alternating series,
Absolute and Conditional Convergence, Leibnitz test. Power series: radius of
convergence of power series, Taylors and Maclaurins Series, Formulae for remainder
term in Taylor and Maclaurin series, Error estimates. (Scope as in Chapter 8, Sections
8.1 8.10 of Reference 2).
Linear Algebra: Concept of linear independence and dependence, Rank of a matrix: (7)
Row Echelon form, System of linear equations: Condition for consistency of system
of linear equations, Solution by Gauss elimination method. Inverse of a matrix: Gauss
Jordan elimination method (Scope as in Chapter 6, Sections 6.3 6.5, 6.7 of Reference
1).
(7)
Eigen values, eigen vectors, Cayley Hamilton theorem (statement only). Similarity of
matrices, Basis of eigenvectors, diagonalization (Scope as in Chapter 7, Sections 7.1,
7.5 of Reference 1).
SECTION-B
Complex Functions: Definition of a Complex Function, Concept of continuity and (8)
differentiability of a complex function, Cauchy Riemann equations, necessary and
sufficient conditions for differentiability (Statement only). Study of complex functions:
Exponential function, Trigonometric functions, Hyperbolic functions, real and
imaginary part of trigonometric and hyperbolic functions, Logarithmic functions of a
complex variable, complex exponents (Scope as in Chapter 12, Sections 12.3 12.4,
12.6 12.8 of Reference 1).
Laurent Series of function of complex variable, Singularities and Zeros, Residues at (7)
simple poles and Residue at a pole of any order, Residue Theorem (Statement only) and
its simple applications (Scope as in Chapter 15, Sections 15.1 15.3 of Reference 1)
Conformal Mappings, Linear Fractional Transformations (Scope as in Chapter 12, (8)
Sections 12.5, 12.9 of Reference 1).
RECOMMENDED BOOKS
S. No.
NAME
AUTHOR(S)
E. Kreyszig
Calculus
G. B. Thomas, R. L.
Finney
Michael D. Greenberg
R. V. Churchill, J. W.
Brown
Linear Algebra
PUBLISHER
SYLLABUS
B.E. IN BIOTECHNOLOGY
FOURTH SEMESTER
Course Code
Course Title
Type of Course
LT P
Credits
Course Assessment Methods
End Semester Assessment (University Exam.)
Continuous Assessment (Sessional)
Course Prerequisites
Course Objectives
Course Outcome
BIO 411
Molecular Biology (Theory)
Core
400
4
50
50
Student should have prior knowledge of
structure, functions and importance of basic
biomolecules such as DNA, RNA and proteins
1. To understand the chromosomal organization
of DNA, its replication, transcription and
translation process
2. To understand the process of cell cycle
regulation
3. To understand about signal transduction
process
4. To understand application of cell and
molecular biology in medicine
1. Student learn the basics of DNA, its
packaging, its replication, transcription and
translation process
2. Student learn the process of cell cycle
regulation
3. Student learn about signal transduction
process
4. Student learn about application of cell and
molecular biology in medicine
Note: The Semester question paper of a subject be of 50 Marks having 7 questions of equal marks.
First question, covering the whole syllabus and having questions of conceptual nature, be
compulsory. Rest of the paper will be divided into two parts having three questions each and the
candidate is required to attempt at least two questions from each section.
SECTION-A
Introduction-Chromosome, chromatin, gene for understanding molecular processes
(1)
DNA replication-Unit of replication, enzymes involved, replication process (initiation, (6)
elongation and termination) in Prokaryotes and Eukaryotes, fidelity of replication,
extrachromosomal replicons
(1)
RECOMMENDED BOOKS
S No.
1
2
Title
Basic Molecular and Cell
Biology
Gene VIII
Authors
Publisher
Latchman,D., 2006 Black Well pub
Schaums Outline of
Theory and Problems of
Molecular and Cell
Biology,
Molecular
Biotechnology,
Principles and
applications of
recombinant DNA
Stansfield, W. D.,
Colome, J.S. and
Cano, R. J., 2004
Edition
3rd
edition
Oxford
University Press,
New York.
Tata McGraw
Hill
American Society
for
Microbiology
Press,
Washington D.C
Second
edition,
Course Code
Course Title
Type of Course
LT P
Credits
Course Assessment Methods
End Semester Assessment (University Exam.)
Continuous Assessment (Sessional)
Course Prerequisites
Course Objectives
Course Outcome
BIO 461
Molecular Biology (Practical)
Core
002
1
00
50
Student should have knowledge about
structure, function and regulation of DNA,
RNA and proteins biomolecules in eukaryotic
and prokaryotic systems
1. To understand basic techniques used for
isolation of RNA, DNA and protein
2. To
understand
the
process
of
transformation and selection of clones in
recombinant DNA technology
1. Student learn the basic techniques used for
isolation of RNA, DNA and protein
2. Student learn the technique of clones
selection used during recombinant DNA
technology
SYLLABUS
List of Experiments:
1. Genomic DNA extraction from yeast cells using mechanical disruption and resolution of
DNA on agarose gel.
2. Total RNA extraction from yeast cells.
3. Resolution of isolated RNA on formaldehyde agarose gel.
4. To study cell cycle arrest in onion root tip cells by treatment with colchicines.
5. Study of bacterial lac operon regulation in bacterial cells using IPTG as an inducer
6. Generation of auxotrophic markers in yeast by UV induced mutagenesis
7. Extraction and separation of total proteins from bacterial cells/animal cell using onedimensional SDS gel electrophoresis method
Course Code
Course Title
Type of Course
LT P
Credits
Course Assessment Methods
End Semester Assessment (University Exam.)
Continuous Assessment (Sessional)
Course Prerequisites
BIO 412
Thermodynamics
Core
400
4
50
50
Process Calculations, Physical Chemistry,
Mathematics and Biochemistry
1. To learn the fundamentals of
Course Objectives
thermodynamics, understand various
forms of energy including heat and work.
2. Application of laws of thermodynamics.
3. Study different refrigeration cycles.
4. Understand the criteria of phase
equilibrium for a pure substance and
mixtures.
5. Understanding of the quantitative aspects
of chemical reaction equilibrium
1. Able to calculate changes in enthalpy,
Course Outcome
internal energy and entropy for ideal
gases.
2. Calculating heat and work requirements
for different processes.
3. Estimation of thermal efficiency and work
for refrigeration cycles.
4. Able to make phase equilibrium
calculations.
5. Able to calculate the composition at
equilibrium for single reactions in a single
phase as a function of temperature and
pressure.
SYLLABUS
Note: The semester question paper of a subject be of 50 Marks having 7 questions of equal marks.
First question, covering the whole syllabus and having questions of conceptual nature, be
compulsory. Rest of the paper will be divided into two parts having three questions each and the
candidate is required to attempt at least two questions from each section.
SECTION-A
Review: state functions, types of systems, internal energy, heat and work, reversible and
(8)
irreversible processes, first laws of thermodynamics and its application, Heat capacities,
Heat effects during phase change, reaction, formation, combustion and mixing.
(4)
(7)
(12)
Chemical Equilibrium: chemical reaction equilibrium; standard free energy change and
equilibrium constant, effects of temperature on free energy change; equilibrium constant;
equilibrium conversion.
(10)
(4)
S.
No.
1
NAME
AUTHOR(S)
PUBLISHER
J.M.Smith, H.C.VanNess
and M. Abbott
Tata McGrawHill.
Y.V.C.Rao
University Press
Prentice Hall
Course Code
Course Title
BIO 413
Chemical Reaction Engineering (Theory)
Type of Course
LT P
Credits
Course Assessment Methods
End Semester Assessment (University Exam.)
Continuous Assessment (Sessional)
Course Prerequisites
Core
400
4
Course Objectives
50
50
Knowledge of material balance equation and
mathematical equations.
1. To understand the characteristics of
kinetics of chemical reactions.
2. To study different types of ideal reactors.
3. To gain understanding of single and
multiple reactions.
4. To follow various aspects of biochemical
kinetics.
SECTION-B
Thermal Characteristic of Reactors: Optimum temperature progression, Adiabatic (6)
Operations.
Design for Multiple Reactions: Qualitative and Quantitative Product distribution for (10)
NAME
AUTHOR(S)
PUBLISHER
Levenspiel,O
Foggler, H. S.
(PHI), 4th
edition, 2006.
Smith, J.M.
(Mc-Graw Hill)
than 3rd Ed.
digitized in
2007.
4.
McGraw Hill
Chemical
Engineering
Series.
Course Code
Course Title
BIO463
Chemical Reaction Engineering (Practical)
Type of Course
LT P
Credits
Course Assessment Methods
End Semester Assessment (University Exam.)
Continuous Assessment (Sessional)
Course Prerequisites
Core
002
1
Course Objectives
Course Outcome
00
50
Knowledge of concepts of chemical reaction
engineering theory.
1. To provide students with hands on training
of different types of chemical reactors.
2. To compare performances of different
reactors under different conditions.
1. Students learn to operate different types of
reactors.
2. Students learn to analyze data generated
as a result of various reactor operations.
SYLLABUS
List of Experiments:
1. Kinetics studies for a non-catalytic reaction in a shake-flask using Integral method.
2. Kinetic studies in a batch reactor at a) constant temperature b) different temperatures.
3. Kinetic studies in a plug flow reactor.
4. Kinetic studies in a CSTR at a) constant temperature b) different temperatures.
5. RTD studies in CSTR.
6. Dispersion number for packed bed reactor.
7. Kinetic studies in a semi-batch reactor.
Course Code
Course Title
Type of Course
LT P
Credits
Course Assessment Methods
End Semester Assessment (University Exam.)
Continuous Assessment (Sessional)
Course Prerequisites
BIO 414
Industrial Biotechnology
Core
400
4
50
50
Students should have done basic courses in
Microbiology and Biochemistry
1. To make the students understand the role
Course Objectives
of diverse organisms in industrial
productions, their growth requirements
and culture preservation.
2. To learn the methods applied for strain
development.
3. To understand the microbial production
process for of a wide range of products
having varied applications.
4. To learn the stabilization methods of
industrial enzymes, immobilization of
enzymes
and
their
role
in
biotransformations.
1. The students will get knowledge of the
Course Outcome
role of large number of organisms in
processes for microbial productions.
2. Students
will
learn
the
strain
development and culture preservation
techniques applied in industry.
3. Students will understand the intricacies
of the production process for each class
of compounds and the applications of a
range of products.
4. Knowledge of enzyme applications,
stability and immobilization methods
required in industry.
SYLLABUS
Note: The Semester question paper should be of 50 Marks having 7 questions of equal marks.
First question, covering the whole syllabus and having questions of conceptual nature, be
compulsory. Rest of the paper will be divided into two parts having three questions each and the
candidate is required to attempt at least two questions from each section.
SECTION-A
Industrially important microbes (E. coli, Bacillus, Actinomyces, Saccharomyces ).
4
Preparation of an ideal growth medium for production of biomass and a microbial 4
product, synthetic and crude media, prerequisites for preparation of a media.
Strain improvement by genetic means
5
Culture preservation
Classifications of microbia1 products.
Microbial fermentations, Introduction to design of fermenters.
SECTION-B
Microbial production processes and applications of the following industrially important
classes of products
a) Organic acids and solvents: citric acid, lactic acid, acetone and butanol
b) Antibiotics: Classification and production of penicillin, streptomycin
c) Enzymes: Amylases, proteases
d) Flavoring agents : nucleosides and nucleotides.
e) Other upcoming productions such as dextran and carotene.
Microbial enzymes, their stability and enzyme immobilization methods.
2
2
5
4
4
3
2
4
4
RECOMMENDED BOOKS
S.
No.
1.
NAME
AUTHOR(S)
PUBLISHER
Industrial Microbiology
L.E. Casida
2.
3.
M.J. Waites et al
Blackwell
Science Ltd.
London 2002
Course Code
Course Title
Type of Course
LT P
Credits
Course Assessment Methods
End Semester Assessment (University Exam.)
Continuous Assessment (Sessional)
Course Prerequisites
Course Objectives
BIO 464
Industrial Biotechnology (Practical)
Core
002
1
00
50
Experience of handling microbial cultures and
biochemical analysis.
1.To have practical training of the concepts
and topics done in theory.
2.To get practical training in studying the
growth curve and utilization of carbon
source in media by bacterial/ yeast
cultures.
3.To get practical training in media
preparation, cell counting and product
formation in bacteria/yeast.
Course Outcome
1.Practical Exposure in handling/ culturing
of
microorganisms,
cell
counting,
measurement of product formation with
respect to time of growth and by
biochemical assay of product formation.
2.Practical Experience of identification
and preservation of microbial cultures.
SYLLABUS
List of Experiments:
1
To identify bacteria on the basis of Grams Staining.
2
To study the growth of E.coli / bacillus in broth media and plot the growth curve.
3
To determine the cell count of S. cerevisiae using Neubauer chamber.
4
To determine the concentration of glucose by DNSA reagent and plot the standard
curve for glucose.
5
To study the utilization of glucose by S. cerevisiae/ Bacillus subtilis.
6
To plot a standard curve of ethanol by using dichromate oxidation method.
7
To study the production of ethanol during the growth of S. cerevisiae.
8
To identify industrially important fungi from slides.
9
To perform cryopreservation of bacteria/ yeast.
Course Code
Course Title
Type of Course
LT P
Credits
Course Assessment Methods
End Semester Assessment (University Exam.)
Continuous Assessment (Sessional)
Course Prerequisites
BIO 415
Immunology and Immunotechnology (Theory)
Core
400
4
50
50
Student should have prior knowledge of
structure and functions of vital organs of human
body
1. To understand the basis of Immunology, cells
Course Objectives
and tissues of immune system
2. To understand the basic of antigen and
antibodies and their generation
3. To understand about importance of antigen
processing,
presentation
and
Major
Histocompatiblity Complex
To understand the details of vaccines
1. Students learn the basis of Immunology,
Course Outcome
cells and organs of immune system
2. Student learn about antigen, antibodies and
their generation
3. Student learn about importance of antigen
processing,
presentation
and
Major
Histocompatiblity Complex
4. Students learn about the Antigen-antibody
reactions so to understand the details of
vaccines.
SYLLABUS
Note: The Semester question paper of a subject be of 50 Marks having 7 questions of equal marks.
First question, covering the whole syllabus and having questions of conceptual nature, be
compulsory. Rest of the paper will be divided into two parts having three questions each and the
candidate is required to attempt at least two questions from each section.
SECTION-A
Introduction- Introduction and historical perspectives of immune system.
(2)
Cell and Tissues of immune system: Lymphoid cell, mononuclear cell, granulocytes, (4)
mast cells, dendritic cells, primary lymphoid organs, lymphatic system, secondary
lymphoid organs
Antigens and Haptens: Immunogenecity, chemical composition, susceptibility to (4)
antigen processing, immunogen dosage and route of administration, haptens, adjuvants.
Antibody Structure, Function and Diversity: Basic structure, Immunoglobulin (6)
domains, classes.
(3)
(4)
(4)
(3)
(3)
(4)
(1)
(3)
(4)
RECOMMENDED BOOKS
S.
No.
1.
Title
Authors
Publisher
Edition
Kuby Immunology
W.H.
Freeman
& Co., NY
6th
edition
2.
Goldsby, Kindt,
T.J., and
Osborne, B. A.
2000
George Pinchuk,
2004
Tata McGraw
Hill Edition
1st
edition,
Abbas, A. K.,
Lichtman, A. H.
& Pober, J. S.,
2000
I.M. Riott, 2000
Saunders Elsevier
6th
edition
ELBS/ Blackwell
Scientific
Publications, U.K
11th
edition
Essential Immunology
BIO 465
Immunology
(Practical)
Core
002
1
Course Code
Course Title
Type of Course
LT P
Credits
Course Assessment Methods
End Semester Assessment (University Exam.)
Continuous Assessment (Sessional)
Course Prerequisites
Course Objectives
Course Outcome
and
Immunotechnology
00
50
Theory of
Immunology and Immunotechnology
1. To understand basic techniques used for
isolation and characterization of different
cells of the immune system
2. To understand various antigen and
antibodies reactions
1. Student learn about isolation, separation
and quantification of various cells and
biomolecules of immune system present
in blood
2. Student learn about the various antigen
and antibodies reactions
SYLLABUS
List of Experiments:
1. TLC and DLC for blood samples.
2. Determination of cell number (viable/non-viable).
3. Separation of lymphocytes cells from blood
4. Determination of blood group antigens by hemeagglutination assay
5. Radial immunodiffusion Assay.
6. Ouchterlony Double Diffusion Assay.
7. Characterization of immunobiologicals by ELISA.
SYLLABUS
B.E. IN BIOTECHNOLOGY
FIFTH SEMESTER
Course Code
BIO 501
Course Title
Type of Course
LT P
Core
400
Credits
Course Outcome
50
50
Introductory biochemistry and general reaction
kinetics.
1. To introduce the basic concepts and
different types of enzyme.
2. To understand the mechanisms of
enzymatic reaction.
3. To understand about enzyme inhibitors and
activators; effect of pH and temperature.
4. To
understand
about
enzyme
immobilization and its applications.
5. To understand about enzymatic reactors for
batch/continuous and biochemical reaction
in enzymatic reactors.
1. Learn about basic concepts and kinetic
reaction of enzyme.
about various methods of
Immobilization and micro-environmental
effect.
3. Understand
about
reactors
for
batch/continuous enzymatic processing,
choice of reactor type; idealized enzyme
reactor systems.
4. Understand about steady state analysis of
mass transfer and biochemical reaction in
enzyme reactors bio-process design.
2. Understand
SYLLABUS
Note: The Semester question paper of a subject be of 50 Marks having 7 questions of equal marks.
First question, covering the whole syllabus and having questions of conceptual nature, be
compulsory. Rest of the paper will be divided into two parts having three questions each and the
S. No.
NAME
AUTHOR(S)
PUBLISHER
Biochemical Engineering
Fundamentals
Bioprocess Engineering Basic
Concepts
Enzymes: Biochemistry,
Biotechnology, Clinical Chemistry
Enzyme Kinetics:
A Modern Approach
McGraw Hill
Publications.
Prentice Hall digitized.
Alejandro g.
Marangoni
2
3
4
Course Code
BIO 551
Course Title
Type of Course
LT P
002
Credits
00
50
Enzyme Engineering & Technology
(Theory)
Course Objectives
Course Outcome
1.
2.
SYLLABUS
List of Experiments:
1. To find out enzyme activity.
2. To study the effect of substrate concentration on enzyme.
3. To find out Vmax and Km of enzyme.
4. To study the effect of temperature on enzyme.
5. To study the effect of pH on enzyme.
6. To find out half life of enzyme.
7. To study the Enzyme immobilization by sodium alginate method and find out
immobilized enzyme activity.
Course Code
Course Title
Type of Course
LT P
Credits
Course Assessment Methods
End Semester Assessment (University Exam.)
Continuous Assessment (Sessional)
Course Prerequisites
Course Objectives
BIO 502
Bioprocess Engineering
Core
400
4
50
50
Chemical reaction engineering concepts and
basics of microbiology.
1.
To understand the characteristics of
2.
3.
4.
Course Outcome
1.
2.
3.
4.
SYLLABUS
Note: The Semester question paper of a subject be of 50 Marks having 7 questions of equal marks.
First question, covering the whole syllabus and having questions of conceptual nature, be
compulsory. Rest of the paper will be divided into two parts having three questions each and the
candidate is required to attempt at least two questions from each section.
SECTION-A
(10)
Kinetics of microbial growth, substrate utilization and product formation in Batch,
Fed-batch and continuous processes.
Rheology of fermentation fluids and Scale-up concepts.
(5)
(8)
SECTION-B
Sterilization of media: design of heat sterilization processes; kinetics; Sterilization in
place and Cleaning in place concepts.
(6)
(4)
(6)
(6)
RECOMMENDED BOOKS
S.
No.
1
NAME
AUTHOR(S)
PUBLISHER
Orgamon
Press., 2nd
edition,1995.
3rd Edition,
2003, Springer
US.
1999 ASM
Press,
Washington DC
Reprinted 2010.
Pauline M. D.
Academic Press
An imprint of
Elsevier.
Prentice
Hall.2002
Course Code
Course Title
BIO 552
Bio-Process Engineering (Practical)
Type of Course
Core
LT P
Credits
Course Assessment Methods
End Semester Assessment (University Exam.)
Continuous Assessment (Sessional)
Course Prerequisites
Course Objectives
002
1
Course Outcome
00
50
Bio-Process Engineering (Theory)
1. Impart the knowledge to students, how to
study microorganisms in different
environmental conditions.
2. Physical and chemical transitions on
microbial cultures and impact of these
factors on final product formation.
1. Students will learn about the concepts of
growth kinetics.
2. Importance of nutritional requirements for
getting desired properties of microbes.
3. Understanding of metabolic changes in
bacteria during oxic and anoxic
environmental conditions.
4. Bioreactor functioning in different
physical conditions and final product
formation.
5. To estimate oxygen requirements by
microbes by applying numerical methods.
SYLLABUS
List of Experiments:
1. Study of different phases of microbial growth; Estimation of cell mass; Growth rate;
mass and energy balance in a typical bioconversion process.
2. Concept of limiting nutrient and effect of its concentration on cell growth.
3. Study of growth inhibition kinetics.
4. Comparison between aerobic and anaerobic bioconversion processes.
5. Power consumption in a fermentation process and its correlation with rheology of the fermentation
fluid ; effect of speed on the mixing time in a bioreactor.
6. Estimation of kLa in a fermentation process.
Course Code
Course Title
Type of Course
LT P
Credits
Course Assessment Methods
End Semester Assessment (University Exam.)
Continuous Assessment (Sessional)
Course Prerequisites
Course Objectives
BIO503
Animal Cell Culture & Bio-Technology
Core
400
4
50
50
Student should have knowledge of cell, cell
structure, function and behavior
1. To understand the biology of animal cell culture
2.
3.
Course Outcome
4.
1.
2.
3.
4.
SYLLABUS
Note: The Semester question paper of a subject be of 50 Marks having 7 questions of equal marks.
First question, covering the whole syllabus and having questions of conceptual nature, be
compulsory. Rest of the paper will be divided into two parts having three questions each and the
candidate is required to attempt at least two questions from each section.
SECTION-A
Animal Cell metabolism, regulation and nutritional requirement;
Animal cell growth characteristics and kinetics; nutrients, substrate and product
transport through mammalian cell;
Primary & secondary culture; cell culture in continuous
Perfusion and hollow-fiber reactor; mass transfer in mammalian cell culture;
Scale-up of cell culture processes; case studies.
SECTION-B
Gene transfer in animal cells and its applications;
Contamination & cyno presentation; Transgenese and transgenic animals including live
stock;
Transgenics as bioreactors ; Biotechnology or aquaculture, silkmoth, past control;
Biodiversity, characterization, conservation; In vitro fertilization, embryotransfer
technology;
(5)
(3)
(6)
(4)
(4)
(8)
(6)
(4)
(4)
RECOMMENDED BOOKS
S.
Title
No.
1.
Animal Biotechnology
2.
(1)
Authors
Publisher
Acadamic Pr.
Edition
6th
edition
Oxford University 3rd
Press, New York. edition
Course Code
Course Title
Type of Course
LT P
Credits
Course Assessment Methods
End Semester Assessment (University Exam.)
Continuous Assessment (Sessional)
Course Prerequisites
Course Objectives
Course Outcome
BIO 553
Animal Cell Culture & Bio-Technology
(Practical)
Core
002
1
00
50
Animal Cell Culture & Bio-Technology
(Theory)
1. To understand basic techniques used
isolating animal cell culture
2. To understand basic techniques used
culturing and subculturing cells
establishing animal cell culture
1. Learn the basic techniques used
isolating animal cell culture
2. Learn the basic techniques used
culturing and subculturing of cells
establishing animal cell culture
SYLLABUS
List of Experiments:
1.
Preparation of cell culture medium.
2.
3.
4.
5.
6.
7.
for
for
for
for
for
for
Course Code
Course Title
Type of Course
LT P
Credits
Course Assessment Methods
End Semester Assessment (University Exam.)
Continuous Assessment (Sessional)
Course Prerequisites
BIO 504
Transport Phenomena
Core
400
4
50
50
Mathematical ability to solve analytically
simple first and second order differential
equations, concepts of heat and mass transfer,
fluid flow and Process Calculations
1. To provide engineering students the
Course Objectives
fundamentals to solve problems involving
transport of momentum, energy and mass
in biological and other systems using a
unified approach.
1. Set up shell balance for conservation of
Course Outcome
momentum, energy and mass, employ
these equations to obtain desired profiles
for
velocity,
temperature
and
concentration.
2. Build the fundamentals for developing
mathematical models for a given process.
3. Appreciate relevance of transport
principles in diverse applications of
chemical, biological and material science
and engineering.
SYLLABUS
Note: The semester question paper of a subject be of 50 Marks having 7 questions of equal marks.
First question, covering the whole syllabus and having questions of conceptual nature, be
compulsory. Rest of the paper will be divided into two parts having three questions each and the
candidate is required to attempt at least two questions from each section.
SECTION-A
Transport of momentum, heat and mass by molecular motion-Newtons law of viscosity,
Fouriers Law of heat conduction, Ficks Law of Diffusion.
Transport properties-Viscosity, Thermal Conductivity and mass diffusivity.
Development of mathematical models of transfer processes through shell momentum
balance for solving specific problems of transport of momentum in laminar flow or in
(3)
(10)
(5)
Emphasis on the analogy between momentum heat and mass transfer with respect to
transport mechanism and governing equations.
(3)
SECTION-B
Development of mathematical models of transfer processes through shell energy balance
and shell mass balance for solving specific problems of transport of heat and mass in one
dimension.
(10)
Development of general differential equations for heat transfer and mass transfer and their
applications in solving one-dimensional steady state and unsteady state problems of heat
and mass transfer.
(10)
Dimensional Analysis.
(4)
RECOMMENDED BOOKS
S No. NAME
1
Transport Phenomena
AUTHOR(S)
R.B.Bird, W.E.Stewart
PUBLISHER
John Wiley and
Sons
Course Code
Course Title
Type of Course
LT P
Credits
Course Assessment Methods
End Semester Assessment (University Exam.)
Continuous Assessment (Sessional)
Course Prerequisites
Course Objectives
Course Outcome
BIO 554
Transport Phenomena Practical
Core
002
1
00
50
Transport Phenomena (Theory)
1. To demonstrate and validate the theoretical
concepts.
1. Application of the theoretical concepts.
SYLLABUS
List of Experiments:
1. Determination of density of liquids using pycnometer.
2. Measurement of viscosity of liquids
3. Thermal Conductivity of solids
4. Heat transfer in natural convection.
5. Heat transfer in forced convection.
6. Determination of mass transfer coefficient for vaporization of naphthalene in air.
7. Determination of mass transfer coefficient as a function of gas mass velocity in a wetted
wall column.
Course Code
Course Title
Type of Course
LT P
Credits
Course Assessment Methods
End Semester Assessment (University Exam.)
Continuous Assessment (Sessional)
Course Prerequisites
Course Objectives
Course Outcome
BIO505
Bio-Process Technology
Core
400
4
50
50
Knowledge of the various eukaryotic and
prokaryotic living systems and the diversity
of their metabolic end products
1. Comparison of bioprocess technology over
chemical technology
2. Knowledge of various substrates to be used
in different bio-productions
3. To study the production, importance and
application aspects of different industrially
important bio-products
4. To understand the nature, treatment and
disposal of waste generated from a
bioprocess industry
1. The course would impart to students
knowledge about various components of
bioprocess technology and its benefit over
chemical technology
2. Knowledge and identification of substrates
and their judicious use for designing media
for carrying out different bioprocesses and
improving their overall efficiency
3. Knowledge of the production of different
bio-products of scientific and commercial
interest and ability to design different
bioprocesses and improve their overall
efficiency.
4. Students learn the importance and methods
of efficient disposal of biowaste
SYLLABUS
Note: The Semester question paper of a subject be of 50 Marks having 7 questions of equal marks.
First question, covering the whole syllabus and having questions of conceptual nature, be
compulsory. Rest of the paper will be divided into two parts having three questions each and the
candidate is required to attempt at least two questions from each section.
SECTION-A
Characteristics and comparison of bioprocess technology with chemical technology
4,2,2
4,2,2
2,2,2,2,3
2,3
6,2
RECOMMENDED BOOKS
S. No.
NAME
AUTHOR(S)
PUBLISHER
Brock, T.D
Comprehensive Biotechnology
Young, M.Y.
Bio-Technology, A Handbook of
Industrial Microbiology
Crueger, W. and
Crueger, A
Cambridge
University Press.
(1992)
Smaeur Associate
(1990) IInd ed.
Wiley eastern
Ltd. (1989)
IInd ed.
Pergamon Press
(1985) Vol. 1-4
Ponima Pub.
(2000) IInd ed
Michael C.
F and Stephen W. D.
Casida, L.E.
Wiley
Biotechnology
Encyclopedias,
(1999) 5 Volume
Set.
Course Code
Course Title
Type of Course
LT P
Credits
Course Assessment Methods
End Semester Assessment (University Exam.)
Continuous Assessment (Sessional)
Course Prerequisites
Course Objectives
BIO506
Bioinstrumentation
Core
400
4
50
50
Knowledge of human physiology, Basics of
signal and systems, Basics of electrical and
electronic instrumentation and measurement
1. The course aims at introducing the students
to the basic principle, design and
application of biomedical instruments.
2. To make them aware of the concept of
biomedical instruments.
Course Outcome
SYLLABUS
Note: The Semester question paper of a subject be of 50 Marks having 7 questions of equal marks.
First question, covering the whole syllabus and having questions of conceptual nature, be
compulsory. Rest of the paper will be divided into two parts having three questions each and the
candidate is required to attempt at least two questions from each section.
SECTION-A
Introduction: Problems unique to bioinstrumentation; Lab View: A graphical programming
language for virtual instrumentation
Basic Principles: Review of system concepts input/output characteristics, the black box signals 2
linear, time-invariant systems static characteristics dynamic characteristics time versus
frequency domain analysis
Fourier Analysis; Fourier transforms of common and important signals windowing Discrete 5
Fourier Transforms The Fast Fourier Transform Spectrum Analyzers windows Frequency
Analysis transfer functions, frequency response magnitude and phase functions signals through
systems 'ideal' and 'best' systems time vs frequency domain filters how to measure frequency
response in the laboratory
Sample systems in the time and frequency domains; 0th, 1st, and 2nd order systems; Non-ideal 2
systems noise and signal-to-noise ratio; non linearities and distortion products
Wave-analyzer synchronous [lock-in] detection modulator, demodulator digital techniques 7
analog to digital conversion signal averaging V. Transducers and associated electronics
displacement transducers resistive strain gages bridge circuits capacitive displacement
transducers piezoelectric transducers optical transducers temperature transducers
Blood pressure, pressure transducers (manometers) extra-vascular techniques blood sounds 2
stethoscopes intra-vascular techniques catheter-manometers
Blood Volume and Flow indicator-dilution methods , electromagnetic flowmeters, ultrasonic 2
methods
SECTION-B
Bioelectric Potentials-Electrophysiology
8
Origins of biopotentials dipoles remote versus local potentials muscle and nerve cells,
intracellular potentials resting potential action potential stimulators discriminators histograms
remote potentials evoked potentials; example: evoked potential audiometry
Electrodes electrode interfaces fluid-fluid salt-fluid metal-fluid surface (skin) electrodes metal 3
micro-electrodes glass micro-electrodes microelectrode preamplifiers
Recording and Stimulating Systems, Putting it all together interference minimization and 4
rejection stimulus isolation shielding grounding ground-loops; Sample recording system:
electrocardiogram; ECG instrumentation
Other cardiac devices ; Pacemakers power and pulse sources electrodes; Defibrillators
Introduction to medical imaging
5
3
RECOMMENDED BOOKS
S. No.
1
NAME
AUTHOR(S)
PUBLISHER
Khandpur, R.S.
Webster, G. J.
Sawhney A K
4
5
Murthy D V S
Communication Systems
S. Haykin
Tenth
Edition
Wiley India
Limited, 4th
Edition
BIO507 Training of 4-6 weeks after 4th semester exams: 50 Marks Credits: 1
SYLLABUS
B.E. IN BIOTECHNOLOGY
SIXTH SEMESTER
Course Code
Course Title
Type of Course
LT P
Credits
Course Assessment Methods
End Semester Assessment (University Exam.)
Continuous Assessment (Sessional)
Course Prerequisites
BIO 601
Recombinant DNA Technology
Core
400
4
50
50
Student should have prior knowledge of
structure, functions and application of DNA,
RNA and proteins
1. To understand the basic concept of gene
Course Objectives
cloning with
basis of isolation and
purification of different types of DNA
molecules
2. To understand about different vector
molecules and enzymes used in gene cloning
experiments
3. To understand about gene expression,
regulation and protein-protein interaction
4. To study the applications of recombinant
DNA technology in the fields of Medicine,
Agriculture, Forensic and Environment
1. Learn the basis of isolation and purification
Course Outcome
of different types of DNA molecules
2. Learn about different vector molecules and
enzymes used in gene cloning experiments
3. Learn about gene expression, regulation and
protein-protein interaction
4. Learn the applications of recombinant DNA
technology in the fields of Medicine,
Agriculture, Forensic and Environment
SYLLABUS
Note: The Semester question paper of a subject be of 50 Marks having 7 questions of equal marks.
First question, covering the whole syllabus and having questions of conceptual nature, be
compulsory. Rest of the paper will be divided into two parts having three questions each and the
candidate is required to attempt at least two questions from each section.
SECTION-A
Gene cloning and need to clone a gene; Isolation and purification of plasmid, (10)
chromosomal and genomic DNA from bacterial, plant and animal cells.
Different cloning vectors like plasmids, cosmids, phagemids, shuttle vectors, and other (10)
vectors for plant and animals; enzymes used in recombinant DNA technology like
restriction endonucleases, ligases, polymerases, kinases and phosphatases.
SECTION-B
Cloning of a specific gene; studying gene location and structure; studying gene (15)
expression; expression of foreign genes in research and biotechnology; maximization of
recombinant proteins; brief introduction to sequencing and site directed mutagenesis,
different types of PCR and applications; safety measures and regulations for
recombinant DNA work
A brief introduction to the followings: phage display system, Yeast two hybrid system, (5)
and RNAi technology.
Applications of recombinant DNA technology in the fields of Medicine, Agriculture, (5)
Forensic and Environment
RECOMMENDED BOOKS
S.
Title
No.
1.
Recombinant DNA: Genes and
Genomes, , H.
.W Freeman pub.
2.
Gene Cloning and DNA
analysis
Authors
Publisher
Edition
Watson, J.D.,
et al. (2007)
McGraw Hill
Publications,
3rd edition
Brown, T.A:,
An
Introduction,
Blackwell
Science.
Benjamin,
Cummings,
Pearson
education,
Schweiz AG,
Germany,
Garland
Publishers
(4th ed.)
3.
Watson, J.:
2004.
4.
Alberts, B. et
al.
1994.
(5th ed.)
Course Code
Course Title
Type of Course
LT P
Credits
Course Assessment Methods
End Semester Assessment (University Exam.)
Continuous Assessment (Sessional)
Course Prerequisites
Course Objectives
BIO 651
Recombinant DNA Technology (Practical)
Core
002
1
00
50
Student should have knowledge about various
biomolecules and basic techniques used in
recombinant DNA technology
1. To understand practical aspect of DNA
isolation techniques used in recombinant
DNA technology
2. To understand digestion and ligation of
various DNA molecules for generating
recombinant molecules
1. Learn the practical aspect of DNA
isolation and quantification techniques
used in recombinant DNA technology
2. Learn the use of various enzymes used for
DNA manipulation for generating
recombinant molecules
Course Outcome
SYLLABUS
List of Experiments:
1. In-situ Oligonucleotide synthesis.
2. Extraction of genomic DNA from yeast cells
3. Amplification of DNA using PCR
4. Isolation of plasmid DNA from E.coli
5. Digestion of plasmid DNA by restriction endonuclease
6. Ligation and Transformation assay
7. Induction and expression of a gene cloned in an expression vector in E.coli
Course Code
Course Title
Type of Course
LT P
Credits
Course Assessment Methods
End Semester Assessment (University Exam.)
Continuous Assessment (Sessional)
Course Prerequisites
Course Objectives
Course Outcome
BIO602
Operations Research
Core
400
4
50
50
Student should have the knowledge of real world
problems so that he can formulate it and solve it
1. To give the student experience in
modeling, solving and analyzing
problems using linear programming.
Emphasis is stressed on theory,
applications, and computer usage.
2. Optimization, i.e., "to do things best
under the given circumstances."
3. To improve a quantitative decision
making procedure.
4. To help the decision-maker to select the
key
decision variables that will
influence the overall quality of decisions.
1. Identify and develop operational research
models from the verbal description of the
real system.
2. Understand the mathematical tools that
are needed to solve optimisation
problems.
3. Use mathematical software to solve the
proposed models.
4. Learn about the strategy that a salesman
should follow so that he can travel in all
the destinations in minimum amount of
timeLearn about the Alternate method to
look at linear programming problem
SYLLABUS
Note: The Semester question paper of a subject be of 50 Marks having 7 questions of equal marks.
First question, covering the whole syllabus and having questions of conceptual nature, be
compulsory. Rest of the paper will be divided into two parts having three questions each and the
candidate is required to attempt at least two questions from each section.
SECTION-A
Optimization Problems. Linear Programming:
(2)
Graphical Method Solution of simultaneous linear equations: An overview
Basic solutions, lines and hyperplanes, convex sets, extreme points, convex sets and (1)
hyperplanes
Reduction of any feasible solution to a system of equations to a basic feasible solu
(3)
tion. Simplex Method: The simplex algorithm
Tableau format for simplex computations, Charnes M-method, Two phase method
The revised simplex method
(6)
Duality theory:
(6)
Formulation of the dual problem, Theorems on duality: Weak Duality Theorem,
Strong Duality Theorem, Complementary Slackness Theorem, Dual Simplex Algorithm
(4)
Integer Linear Programming:
Branch and Bound Algorithm, Cutting Plane Algorithm
SECTION-B
Transportation Problem:Initial solution by North-West corner rule, Row minima (4)
method, Column minima method, Matrix minima method, Vogels method. Tableau of
transportation problem, u-v algorithm for solving transportation problem. Degeneracy
in transportation problem
The Assignment Problem:Hungarian Method Traveling Salesman Problem
(4)
Dynamic Programming:Shortest route problem, Knapsack Model, Workforce size
(6)
model, Equipment replacement model, Investment model, Game of chance
CPM and PERT:Network representation, Critical path computations, Construction of
time schedule
Linear programming formulation of CPM, PERT networks
(2)
Basic Queuing Systems:Elements of a queuing model, Pure birth and pure death model, (5)
Generalized Poisson queuing model
RECOMMENDED BOOKS
S.
Title
No.
1.
Linear Programming
2.
OperationsResearch, An
Introduction
Authors
Publisher
Edition
G. Hadley
NarosaPublishing
House, NewDelhi
. Pearson
Education, Delhi
2002
Hamdy A. Taha
Seventh
Edition
Course Code
Course Title
Type of Course
LT P
Credits
Course Assessment Methods
End Semester Assessment (University Exam.)
Continuous Assessment (Sessional)
Course Prerequisites
Course Objectives
Course Outcome
BIO652
Operation Research (Practical)
Core
002
1
00
50
Operation Research (Theory)
1. To give the student experience in
modeling, solving and analyzing
problems using linear programming.
Emphasis is stressed on theory,
applications, and computer usage.
2. Optimization, i.e., "to do things best
under the given circumstances."
3. To improve a quantitative decision
making procedure.
4. To help the decision-maker to select
the key
decision variables that will
influence the overall quality of decisions
1. Identify and develop operational
research models from the verbal
description of the real system.
2. Understand the mathematical tools that
are needed to solve optimisation
problems.
3. Use mathematical software to solve the
proposed models.
4. Learn about the strategy that a salesman
should follow so that he can travel in all
the destinations in minimum amount of
time Learn about the Alternate method
to look at linear programming problem
SYLLABUS
List of Experiments:
Based on the theory
Course Code
Course Title
Type of Course
LT P
Credits
Course Assessment Methods
End Semester Assessment (University
Exam.)
Continuous Assessment (Sessional)
Course Prerequisites
Course Objectives
Course Outcome
BIO 603
Introduction to bioinformatics (Theory)
Core
400
4
50
50
Knowledge of biomolecules like DNA,
RNA, proteins and some fundamentals of
computer.
1. To introduce the students about
objectives and areas of bioinformatics;
genome sequencing projects, basic
concepts of database, types of
databases
2. To develop understanding for using
databases in protein sequence analysis
and nucleic acid sequence analysis
3. To introduce about gene prediction in
prokaryotic and eukaryotes genomes,
prediction of protein secondary
structure, three-dimensional structure
4. To understand concepts of molecular
modeling and use of molecular
graphics packages, computer aided
drug design
1. Students would be able to understand
objectives and areas of bioinformatics
2. The course would also enable them to
understand about genome sequencing
projects, some concepts and types of
databases
3. Students would be able to use
databases for protein and nucleic acid
sequence
analysis,
sequence
homology, gene prediction, protein
structure prediction by various tools
4. The course would also enable them to
understand concepts of drug design
Note: The Semester question paper of a subject be of 50 Marks having 7 questions of equal
marks. First question, covering the whole syllabus and having questions of conceptual nature,
be compulsory. Rest of the paper will be divided into two parts having three questions each and
the candidate is required to attempt at least two questions from each section.
SECTION-A
Introduction to Bioinformatics: History of Bioinformatics; Objectives and areas of (3)
Bioinformatics; Genome sequencing projects; Human Genome Project - history,
techniques and insights.
Introduction to databases: Basic concept of database, Type of databases; Literature (8)
Databases-PUBMED, MEDLINE;Nucleic acid and protein databases- GenBank,
EMBL, DDBJ, SWISS PROT, UNIPROT;Human, animal and plant databasesEnsembl, Genome project TIGR database, Maize GDB etc.Structural databases- PDB,
PDBsum, NDB etc;Motifs and Pattern Databases- PROSITE, Pfam, BLOCKS,
PRINTS etc;Database Retrieval and deposition systems- SRS, Entrez, Bankit, Seqin,
Webin, AutoDep.
(2)
Basic Sequence Analysis: Protein Sequence Analysis- composition, hydropathy,
flexibility, pattern, motif etc; Nucleic acid Sequence Analysis- Composition, motif,
restriction site, primer design etc.
Sequence Homology: Scoring matrices, Local and global alignment concepts, Dot (7)
matrix sequence comparison, Dynamic programming; Statistics of alignment
score;Database searches for homologous sequences- FASTA, BLAST, PSI-BLAST and
PHI-BLAST; Multiple sequence alignment: CLUSTALW, PILEUP; Evolutionary
analysis- Concept of phylogeny and trees, Relationship of phylogenetic analysis to
sequence alignment.
Gene prediction: Gene prediction in prokaryotic and eukaryotes genomes; evaluation (2)
of gene prediction methods.
SECTION-B
Protein structure prediction: Prediction of protein secondary structure from the (7)
amino acid sequence- Chou-Fasman/GOR method, JPRED, PSIPRED, PHD;
Prediction of three-dimensional protein structure-Homology-based structure prediction,
Fold recognition and ab initio methods for structure prediction; Evaluating the success
of structure predictions-CASP and CAFASP.
Introduction to the concepts of molecular modeling: Molecular structure and internal
(8)
energy; Molecular Mechanics; Energy Minimization and related methods for exploring
the energy surface; Molecular Dynamics, Conformational analysis; Use of molecular
graphics packages- Rasmol, MOLMOL, Chimera, Pymol, spdbviewer.
Computer Aided drug design: Drug discovery process; Role of molecular recognition (7)
in drug design; Concepts in Quantitative structure activity relationships (QSAR);
Gene (1)
RECOMMENDED BOOKS
S. No.
1
NAME
AUTHOR(S)
PUBLISHER
Mount D. W.
Cold Spring
Harbor
Laboratory
Press(2004)
Introduction to Bioinformatics
Garland
Publishing Inc.,
New
York(1991)
Willy
Publication
Course Code
Course Title
Type of Course
LT P
Credits
Course Assessment Methods
End Semester Assessment (University Exam.)
Continuous Assessment (Sessional)
Course Prerequisites
Course Objectives
Course Outcome
BIO653
Introduction to Bioinformatics (Practical)
Core
002
1
00
50
Introduction to Bioinformatics (Theory)
1. To develop skills in using various
computational tools available online for
retrieving DNA and Protein sequences,
similarity searches among them,
phylogenetic analysis, gene prediction, 2D
and 3D protein structure analysis
1. Students would be able to study and
analyse biological databases at NCBI,
EBI, Expasy, NBRF-PIR
2. Students would learn to retrieve DNA and
Protein sequences from databases
3. Students would be able to develop skills in
using various computational tools
available online for similarity searches,
phylogenetic analysis, gene prediction, 2D
and 3D protein structure analysis for DNA
and protein sequences
SYLLABUS
List of Experiments:
a) To Study & analyse various biological databases at NCBI, EBI, Expasy, NBRF-PIR
Nucleic acid sequence databases like Gene Bank, EMBL etc.
Protein sequence databases SWISSPROT, UNIPROT etc.
Structural databases- PDB, NBD
b) To retrieve sequences from NCBI/EBI/ExPasy using ENTRZ, SRS
c) Similarity searches using various tools like
BLAST/ FASTA, BLAST N, BLAST P, BLAST X
d) CLUSTALW / Phylogenetic analysis tools
e) To predict gene/ORF for genomic DNA sequences of prokaryotic and eukaryotic origin.
Course Code
Course Title
Type of Course
LT P
Credits
Course Assessment Methods
End Semester Assessment (University Exam.)
Continuous Assessment (Sessional)
Course Prerequisites
Course Objectives
BIO 604
Bioreactor Design & Operation
Core
400
4
50
50
Basics of bioprocess engineering concepts.
1. To understand the characteristics of
biological systems.
2. To study about different types of reactors
and their non-ideal behavior.
3. To gain understanding of control and
instrumentation of various process
parameters.
4. To understand modeling and stability of a
reactor process.
1. Knowledge of different types of
Course Outcome
microorganisms and their effect on the
reactor design.
2. Understanding of performances of
different types of reactors and their nonideality calculation.
3. Knowledge of principles of measurement
of various process parameters and their
control.
4. Learning about the fermentation dynamics
and their stability in terms of operation.
SYLLABUS
Note: The Semester question paper of a subject be of 50 Marks having 7 questions of equal marks.
First question, covering the whole syllabus and having questions of conceptual nature, be
compulsory. Rest of the paper will be divided into two parts having three questions each and the
candidate is required to attempt at least two questions from each section.
SECTION-A
Introduction to reactor design and Kinetics of Bioreactions (review).
(1)
Biological systems: Organism selection; bacterial, yeast and fungal cultures; Effect of (6)
microorganism type and culture characteristics on bioreactor design and operation.
Ideal Reactors: Batch reactor (Closed and fed-batch), continuous reactors; PFTR, (10)
CSTR design equations. Reactors for biomass growth; reactors in series; recycle
reactors; overview of pneumatically agitated bioreactors; Membrane bioreactors and
Photo bioreactors.
RTD in reactors: Models for non-ideal reactors; Tanks in series and dispersion models.
(6)
SECTION-B
Instrumentation and control of various parameters in bioreactors for Dissolved (8)
oxygen, foam,pH, temperature, flow, pressure, microbial biomass, CO2
etc; Methods
of measuring process variables and control systems: Proportional, Proportional integral
and Proportional integral derivative.
(4)
Bioreactor modeling and stability: Fermentation dynamics, Biomass production and (6)
dilution factor, Thermal stability concepts.
Mechanical design Concepts: Application to continuous sterilizers, RTD concepts, (4)
Application of design principles.
RECOMMENDED BOOKS
S.
No.
1
NAME
AUTHOR(S)
PUBLISHER
P.H,
2nd edition, Prentice
Hall.
Dekker. C R C Pr. 1st
edition, 1994. (Eds.)
Bioprocess Engineering
Principles
Pauline M. D.
Academic Press
An imprint of
Elsevier.
Course Code
Course Title
Type of Course
LT P
Credits
Course Assessment Methods
End Semester Assessment (University Exam.)
Continuous Assessment (Sessional)
Course Prerequisites
BIO605
Downstream Processing
Core
400
4
50
50
Knowledge of various bioprocesses of
industrial importance and their components
1. To make students understand the
Course Objectives
difference between upstream and
downstream processing and different
phases of downstream processing of
different bio products
2. To impart knowledge about the various
unit operations involved in the isolation
and extraction of bio-products from the
fermentation broth
3. To learn different
methods
of
concentration, purification and final
polishing of the bio-product before
commercial use
1. Understanding of different stages of
Course Outcome
downstream processing
2. Knowledge of principles and working of
different unit operations for the isolation
and extraction of bio-products
3. Knowledge of different methods and
industrial equipments used for the
concentration, purification and final
polishing of bio-products at the industrial
level
SYLLABUS
Note: The Semester question paper of a subject be of 50 Marks having 7 questions of equal marks.
First question, covering the whole syllabus and having questions of conceptual nature, be
compulsory. Rest of the paper will be divided into two parts having three questions each and the
candidate is required to attempt at least two questions from each section.
SECTION-A
Introduction; An overview of Bioseparation, Separation of cells and other insolubles from 2
fermented broth;
Filtration and microfilteration; Centrifugation (batch, continuous). Designing of centrifuges for
12
Section-B
Electrophoresis and chromatography principles for product purification. Different
18
electophoresis techniques viz. isoelectric focusing, chromatographic techniques viz. paper, gel
filtration, column, ion exchange, affinity, GLC, HPLC. Dialysis, ultrafilteration;
Product polishing: crystallization and drying.
RECOMMENDED BOOKS
S. No.
NAME
AUTHOR(S)
PUBLISHER
Biochemical
Engineering
Fundamentals
McGraw-Hill
Science/Engineering/Math
(1986) 2nd Sub edition
Handbook of Analytical
Techniques
Biotechnology Vol. 3,
4, 5
Gunzler, H. and
Williams, A.
Rehm, H.J. and
Reed, G.
A Biologist's guide
to principles and
techniques of
practical biochemistry
Principles of
Fermentation
Technology
Wilson, K. and
Goulding, K.H.
Stanbury, P.F,
Whitaker, A. and
Hall, S.J.
Butterworth-Heinemann (1999)
2nd ed.
3
4
Course Code
Course Title
Type of Course
LT P
Credits
Course Assessment Methods
End Semester Assessment (University Exam.)
Continuous Assessment (Sessional)
Course Prerequisites
Course Objectives
BIO655
Down Stream Processing (Practical)
Core
002
1
00
50
Down Stream Processing (Theory)
1. To impart to students working knowledge
of different methods and techniques used
during different stages of downstream
processing of different bio-molecules
1. The students learn to isolate, concentrate
Course Outcome
and purify bio-molecules using different
techniques pertaining to downstream
processing
List of Experiments
1. Cell lysis and release of cell contents
2. Use of centrifugation, ultra centrifugation, ultra filtration
3. Lypophilization
4. Crystallization
5. HPLC for bio-separation
Course Code
Course Title
Type of Course
LT P
Credits
Course Assessment Methods
End Semester Assessment (University Exam.)
Continuous Assessment (Sessional)
Course Prerequisites
BIO606
Biomaterials
Core
400
4
50
50
Knowledge of human anatomy and
physiology and basics of immunology
1. The course aims at introducing the
Course Objectives
students to properties of biomaterials
2. To make them aware of different types
of tissue grafts and immunological
response to the grafts
3. To make them understand the utilization
of different classes of synthetic polymers
and biopolymersas implant material in
various organ systems
1. To students learn about the mechanical,
Course Outcome
thermal and surface properties of
biomaterials
2. Learn about the application of synthetic
and biopolymer as implant material for
soft and hard tissues
3. The students learn about the interaction
of implant material with the body tissues
and approaches to prevent rejection of
the implant
SYLLABUS
Note:The Semester question paper of a subject be of 50 Marks having 7 questions of equal marks.
First question, covering the whole syllabus and having questions of conceptual nature, be
compulsory. Rest of the paper will be divided into two parts having three questions each and the
candidate is required to attempt at least two questions from each section.
SECTION-A
Introduction to biomaterials: Characterization of materials, mechanical properties, 4
thermal properties,surface properties and adhesion, Various classes and forms of
biomaterials
Metals:Stainless steel, Cobalt- Chromium Alloys, Titanium based alloys, other metals, 4
metallic corrosion and biological tolerance.
Ceramics :Carbons ,Alumina, Resorbable ceramics, Composites, Ceramic surface 3
analysis.
Synthetic polymers: Polymers in biomedical use, Polyethylene, Polypropylene, 4
NAME
AUTHOR(S)
Biomaterials
Sujata V. Bhat
Biomaterials Science: An
Introduction to Materials in
Medicine
Biomaterials: An
Introduction
PUBLISHER
Alpha Science
International
(2005)2ndedition
Academic Press
(2013) 3rd edition
Springer Science+
Business Media
(2007) 3rd edition.
SYLLABUS
SEVENTH SEMESTER
Course Code
BIO 701
Course Title
Type of Course
Core
LT P
400
Credits
50
50
Course Prerequisites
Course Objectives
Course Outcome
SYLLABUS
Note: The Semester question paper of a subject be of 50 Marks having 7 questions of equal marks.
First question, covering the whole syllabus and having questions of conceptual nature, be
compulsory. Rest of the paper will be divided into two parts having three questions each and the
candidate is required to attempt at least two questions from each section.
SECTION-A
Introduction:
Environmental Pollution: Sources and effects. Biodegradation and (23)
Bioremediation-definitions and examples.
Waste Water Treatment: Aerobic and Anaerobic waste water treatment. Kinetics for waste
water treatment processes. Different type and design of Aerobic and Anaerobic reactors,
Methanogenesis. Emerging biotechnological processes in waste water treatment.
SECTION-B
Solid Waste management: Biodegradation of pollutants, Treatment processes for solid (22)
waste, Use and Management, Bioremediation of contaminant lands. Hazardous wastes: source
management and safety.
Microbial Leaching and Mining: Recovery of metals from solutions, microbes in petroleum
extraction, microbial desulphurization of coal.
Environmental Genetics: Degradative plasmids, release of genetically engineered microbes
in environment.
RECOMMENDED BOOKS
S. No.
NAME
AUTHOR(S)
PUBLISHER
Environmental biotechnology:
Principles and Applications.
Wastewater Engineering:
Treatment and Reuse
McGraw Hill
Publications.
Environmental Biotechnology
T. Srinivas
New Age
International (P) Ltd.
Principles of Environmental
Science &Technology
K. Saravana, S.
Ramachandran
New Age
International (P) Ltd.
Course Code
BIO 751
Course Title
Type of Course
Core
LT P
002
Credits
00
50
Course Prerequisites
Course Objectives
Course Outcome
SYLLABUS
List of Experiments:
1.
2.
3.
4.
5.
6.
7.
Course Code
Course Title
Type of Course
LT P
Credits
Course Assessment Methods
End Semester Assessment (University Exam.)
Continuous Assessment (Sessional)
BIO 702
Food Biotechnology (Theory)
Core
400
4
Course Prerequisites
Course Objectives
Course outcomes
50
50
SYLLABUS
Note: The Semester question paper of a subject be of 50 Marks having 7 questions of equal
marks. First question, covering the whole syllabus and having questions of conceptual nature, be
compulsory. Rest of the paper will be divided into two parts having three questions each and the
candidate is required to attempt at least two questions from each section.
SECTION A
History of Microorganisms in food, Primary sources of Microorganisms in foods, (11)
NAME
AUTHOR(S)
PUBLISHER
1.
Food Microbiology
D.C
Jay. J.M.
2.
Modern Food
Microbiology
3.
4.
Food Microbiology
Food Biotechnology:
M.O.
Gauri Mittal
Cliver, D.D.
Techniques and
Applications
5.
Foodborne Diseases
Course Code
Course Title
Type of Course
LT P
Credits
Course Assessment Methods
End Semester Assessment (University Exam.)
Continuous Assessment (Sessional)
BIO 702
Food Biotechnology (Practical)
Core
002
1
Course Prerequisites
Course Objectives
Course outcomes
00
50
SYLLABUS
List of Experiments:
Microbiological Examination of food/s. Enumeration and detection of food borne organisms.
Estimation of quality of milk-by dye reduction, direct microscopic count, Determination of
diacetyl, titrable acidity in the milk sample.
Course Code
Course Title
Type of Course
LT P
Credits
Course Assessment Methods
End Semester Assessment (University Exam.)
Continuous Assessment (Sessional)
BIO 703
Plant Tissue Culture (Theory)
Core
400
4
Course Prerequisites
Course Objectives
Course outcomes
50
50
SYLLABUS
Note: The Semester question paper of a subject be of 50 Marks having 7 questions of equal
marks. First question, covering the whole syllabus and having questions of conceptual nature, be
compulsory. Rest of the paper will be divided into two parts having three questions each and the
candidate is required to attempt at least two questions from each section.
SECTION A
Introduction, requirements and techniques,
(4)
(5)
(6)
Haploid Production: Zygotic Embryo Culture: Morphogenesis in the culture of seeds with (5)
partially differentiated embryos.
SECTION B
Microsurgical experiments, Morphogenic potential of the embryo callus, practical (5)
applications.
In Vitro Pollination and fertilization: Introduction, Terminology, In-Vitro Pollination, (5)
Applications.
Genetic engineering and production of pathogen free plants: Gene expression, genetic (5)
stability, and field performance
Introduction, Strategies used to optimize product yield, commercial aspects, Germplasm (10)
storage: Introduction, long term Storage, Short or Medium term storage.
RECOMMENDED BOOKS
S.No
NAME
AUTHOR(S)
PUBLISHER
1.
Plant Biotechnology:
The Genetic
2003.
Techniques and
Manipulation of Plants
2.
Experiments
3.
Experiments in Plant
J. Heslop-Harrison, John
Tissue Culture
Course Code
Course Title
Type of Course
LT P
Credits
Course Assessment Methods
End Semester Assessment (University Exam.)
Continuous Assessment (Sessional)
BIO 704
Bioanalytical Techniques (Theory)
Core
400
4
Course Prerequisites
Course Objectives
Course outcomes
50
50
SYLLABUS
Note: The Semester question paper of a subject be of 50 Marks having 7 questions of equal
marks. First question, covering the whole syllabus and having questions of conceptual nature, be
compulsory. Rest of the paper will be divided into two parts having three questions each and the
candidate is required to attempt at least two questions from each section.
SECTION A
Spectrophotometry (UV and Visible): Principle, single beam and double beam (5)
spectrophotometer, factors influencing the absorption spectra, overview of empirical
rules, solvent perturbation method and difference spectroscopy; various applications of
absorption spectroscopy with respect to biotechnology.
Spectrofluorimetry: Principle, significance and various details related to instrumentation
Cellular Totipotency, Somatic Embryogenesis, synthetic seeds
(3)
RECOMMENDED BOOKS
S.No
1.
NAME
AUTHOR(S)
PUBLISHER
Principles and
Techniques of Practical
2000
Biochemistry
2.
Organic Spectroscopy
William Kemp
3.
Physical Biochemistry
Friefelder D
Course Code
BIO 754
Course Title
Type of Course
Core
LT P
002
Credits
00
50
Course Objectives
Course Outcome
SYLLABUS
List of Experiments:
1. To determine maximum wavelength (max) for a given colored solution.
2. To confirm the Beers Lambert law for the given colored solution.
3. To elucidate a structure of a given chemical compound using NMR.
4. To investigate different samples for the elemental composition using X-ray fluorescence.
5. To confirm the presence of different functional groups using FT-IR spectroscopy.
6. Rapid protocol for enzyme detection.
Course Code
BIO 705
Course Title
Type of Course
Core
LT P
004
Credits
00
50
Course Code
BIO 706
Course Title
Credits
00
50
SYLLABUS
EIGHTHSEMESTER
Course Code
BIO 801
Course Title
Type of Course
Core
LT P
004
Credits
00
50
Core courses in Biotechnology
Course Code
BIO 802
Course Title
Type of Course
Core
LT P
400
Credits
Course Assessment Methods
End Semester Assessment (University Exam.)
Continuous Assessment (Sessional)
Course Prerequisites
Course Objectives
Course Outcome
50
50
SYLLABUS
Note: The Semester question paper of a subject be of 50 Marks having 7 questions of equal marks.
First question, covering the whole syllabus and having questions of conceptual nature, be
compulsory. Rest of the paper will be divided into two parts having three questions each and the
23
RECOMMENDED BOOKS
S. No.
NAME
AUTHOR(S)
PUBLISHER
J Andreas S.
WILEY-VCH.
Bommarius, Bettina R.
Riebel.
Biocatalysis and Biodegradation: Lawrence Philip
ASM Press.
Microbial Transformation of
Wackett , C. Douglas
Organic Compounds
Hershberger
Enzymes: Biochemistry,
Trevor Palme
Horwood Publishing
Biotechnology, Clinical
Limited.
Chemistry
Biotransformations
Kurt Faber
Springer
in Organic Chemistry
22
Course Code
Course Title
Type of Course
LT P
Credits
Course Assessment Methods
End Semester Assessment (University Exam.)
Continuous Assessment (Sessional)
Course Prerequisites
BIO 852
Enzyme Catalyzed Organic Synthesis
(Practical)
Core
003
2
00
50
Enzyme Catalyzed Organic Synthesis
(Theory)
1. The course aims to provide the practical
knowledge of Optimization of enzymatic
reactions.
Course Objectives
Course Outcome
SYLLABUS
List of Experiments:
1. To find out specific enzyme activity.
2. To study the Optimization of enzymatic catalysis reaction conditions.
3. To study the effect of organic solvent on enzyme activity
4. To study the enzymatic catalysis reaction with activators.
5. To study the enzymatic catalysis reaction with inhibitors.
6. To study the Product identification using different analytical technique.
7. To study the cell immobilization and enzyme immobilization of various method.
Course Code
Course Title
BIO803
Project Management and Entrepreneurship
Type of Course
LT P
Credits
Course Assessment Methods
End Semester Assessment (University Exam.)
Continuous Assessment (Sessional)
Core
400
4
Course Prerequisites
Course Objectives
Course Outcome
50
50
SYLLABUS
Note: The Semester question paper of a subject be of 50 Marks having 7 questions of equal marks.
First question, covering the whole syllabus and having questions of conceptual nature, be
compulsory. Rest of the paper will be divided into two parts having three questions each and the
candidate is required to attempt at least two questions from each section.
SECTION-A
Project Formulations and Planning ,Private commercial criteria for project choice,
project cycle, feasibility, marketing feasibility
Financing for Projects and financial feasibility
(6)
(6)
(4)
UNIDO and little Mirrlees approaches, UNIDO-IDCAS manual, shadow prices and
conversion factors, applications in India. Planning and scheduling networks
(6)
Critical path, PERT model, CPM model, PERT/cost, resource leveling and allocation.
(3)
SECTION-B
Entrepreneur- Concept on percent - Functions and clarifications of entrepreneurs Characteristics of entrepreneur - Nature and importance of ,entrepreneur
(6)
(6)
(2)
-Entrepreneurship
development
in
other
countries.
(2)
2.
3.
4.
5.
NAME
Fundamentals of
Entrepreneurship and Small
business Management.
Entrepreneurship
AUTHOR(S)
Vasant Desai
Hisrich,Peters and
Shepherd
UNIDO: Guidelines for Project UNIDO
Evaluation, United Nations
Manual for the preparation of
Industrial Feasibility Studies,
United Nations 1978.
Project Apraisal and Planning
in Developing Countries
PUBLISHER
Himalya Publishing
House
Tata McGrawHill
UNIDO
United Nations
(4)
Course Code
BIO 804
Course Title
Type of Course
LT P
Credits
Course Assessment Methods
End Semester Assessment (University Exam.)
Continuous Assessment (Sessional)
Course Prerequisites
Course Objectives
Course Outcome
50
50
Introductory Bioprocess Engineering and
Mathematical Calculation.
1. To introduce the Basic concepts about
kinetic models.
2. To
understand
the
Mathematical
representation of bioprocess.
3. To understand the Development of
compartment and metabolic pathway
models.
4. To introduce the Dynamic simulation of
batch, fed-batch, CSTR and transient
culture metabolism.
5. To introduce the Numerical optimization.
1. Learn about basic concepts of Kinetic
Models.
2. Learn about bioprocess design in a various
systems solve model equation.
3. To learn the dynamic simulation of
metabolic pathway models.
4. To study the mathematical modeling of
batch, Continuous & FedBatch Reactors.
5. To learn about the Numerical integration
techniques for model validation.
SYLLABUS
Note: The Semester question paper of a subject be of 50 Marks having 7 questions of equal marks.
First question, covering the whole syllabus and having questions of conceptual nature, be
compulsory. Rest of the paper will be divided into two parts having three questions each and the
candidate is required to attempt at least two questions from each section.
SECTION-A
Types of kinetic models. Data smoothing and analysis.
23
NAME
AUTHOR(S)
PUBLISHER
Chemical Engineering
Dynamics: Modeling with PC
Simulation
Biological Reaction
Engineering: Dynamic Modeling
Fundamentals with Simulation
Examples.
Bioprocess Engineering Basic
Concepts
Bioprocess Engineering
Principles
Wiley-VCH
P.H. M.L.Shuler ,
F.Kargi
Pauline Doran
3
4
Wiley-VCH
Academic Press
Course Code
Course Title
Type of Course
LT P
Credits
Course Assessment Methods
End Semester Assessment (University Exam.)
Continuous Assessment (Sessional)
Course Prerequisites
Course Objectives
Course Outcome
BIO 854
Modeling and Simulation of Bioprocesses
Practical
Core
003
2
00
50
Modeling and Simulation of Bioprocesses
(Theory)
To demonstrate and validate the theoretical
concepts.
The students shall be able to use computers
for solving the differential models developed
for the defined systems.
SYLLABUS
List of Experiments:
Exercises are conducted in the computational lab using C/C++ language illustrating the
simulation of lumped parameter system models.
Course Code
BIO 805
Course Title
Nanobiotechnology
Type of Course
LT P
Credits
Course Assessment Methods
End Semester Assessment (University Exam.)
Continuous Assessment (Sessional)
Elective
400
4
Course Prerequisites
Course Objectives
Course Outcome
50
50
SYLLABUS
Note: The Semester question paper of a subject be of 50 Marks having 7 questions of equal marks.
First question, covering the whole syllabus and having questions of conceptual nature, be
compulsory. Rest of the paper will be divided into two parts having three questions each and the
candidate is required to attempt at least two questions from each section.
SECTION-A
Cell nanostructure interactions: Surface patterning to control the adhesion of cells using
nanoscale topography and chemical modifications.
Magnetosomes: Magnetotactic bacteria, Magnetite crystals, Magneto-aerotaxis,
Applications Nanotherapeutics: Nanoshells, Dendrimers , Nanoparticles.
Tissue engineering: Significance, Methodology,
Tissue engineering scaffoldscomposition, properties, fabrication, cell seeding and proliferation.
Nanomembranes: Freely suspended nanomembranes.
23
SECTION-B
Bionanodevices: Nanosensors and Actuators. Nanopumps: Fabrication using SOI
wafers. Nonorobots: Developing nanorobots for biomedical applications.
Molecular motors: Surface patterning and the control of motility of the actin/myosin
motor system.
Engineered nanopores:
Classes of nanopores, engineering techniques, potential
applications of nanopores.
RECOMMENDED BOOKS
S. No.
NAME
AUTHOR(S)
PUBLISHER
Nanobiotechnology:Concepts,
Wiley-VCH
A. Mirkin(Eds)
Bionanotechnology: Lessons
David S. Goodsell
Wiley Liss
from Nature
3
Niemeyer(Eds)
Handbook of Nanotechnology
Bharat Bhushan(Ed)
Wiley-VCH
Springer Verlag
22
Course Code
BIO 805
Course Title
Microbial Biodiversity
Type of Course
LT P
Credits
Course Assessment Methods
End Semester Assessment (University Exam.)
Continuous Assessment (Sessional)
Elective
400
4
Course Prerequisites
Course Objectives
Course Outcome
50
50
SYLLABUS
Note: The Semester question paper of a subject be of 50 Marks having 7 questions of equal marks.
First question, covering the whole syllabus and having questions of conceptual nature, be
compulsory. Rest of the paper will be divided into two parts having three questions each and the
candidate is required to attempt at least two questions from each section.
SECTION-A
Microbial biodiversity: definition and introduction, evolution and diversity of
microorganisms.
Physiological and metabolic diversity of microorganisms.
Microbial diversity of major ecosystems.
(7)
(7)
(8)
SECTION-B
Biodiversity & role of microorganisms in plants and animal symbiosis.
Microbial diversity in extreme environments.
(8)
(9)
(6)
RECOMMENDED BOOKS
S. No.
NAME
McGraw-Hill,
Version)
2002
Microbial
AUTHOR(S)
Diversity:
Form
function in Prokaryotes.
3
Microbial
Bioprospecting
Diversity
PUBLISHER
Blackwell
Publishers, 2004
American Society
Microbiology,
2003.