Cultural and Morphological Variability of Isolates Causing Blight of Linseed

International Journal of Agricultural
Science and Research (IJASR)

ISSN(P): 2250-0057; ISSN(E): 2321-0087
Vol. 7, Issue 6, Dec 2017, 361-372
© TJPRC Pvt. Ltd.
CULTURAL AND MORPHOLOGICAL VARIABILITY OF ALTERNARIA LINI
ISOLATES CAUSING BLIGHT OF LINSEED.
A.K.VERMA & R.B.SINGH

Department of Plant Pathology N.D. University of Agricultural &Technology Kumarganj, Faizabad- (U.P.) India
ABSTRACT
Alternaria lini causes blight of linseed in India. The leaves showing typical symptoms of Alternaria blight, in
linseed collected from different part of country and characterized for cultural, morphological, pathogenic and molecular
variations. Alternaria lini colonies varied in respect of their colony colour (whitish, light brown to dark brown), growth
pattern (slow, medium to fast), appearance (cottony, fluffy, feathery to compressed & thin), radial growth (25mm to
60mm), shape (circular), margin (wavy, rough to smooth), zonation (presence or absence), and sporulation (early,
medium to late). Morphological variation in average conidial length (23.26 to 45.72µm), width (6.76 to 17.01µm), number
of septa (1.86 to 5.60) and presence or absence of beak (unbeaked, rudimentary beaked and short beaked) were observed.
Average conidial size ranged between 24x7 to 46x17µm.
Original Article
KEYWORDS: Alternarialini, Linum, Cultural and Morphological Variability
Received: Nov 06, 2017; Accepted: Nov 26, 2017; Published: Dec 02, 2017; Paper Id.: IJASRDEC201749
INTRODUCTION
Linseed (Linumusitatissimum L.), belongs to the family Linaceae and the genus Linum is commonly
known as “Ulsee” or “Tisee”. It contains 20 to 30% protein. Feeding linseed and its oil may be medicated in
hyperlipidemia, to reduce and control arteriosclerosis, thrombosis and mycocardial infraction, by reducing
cholesterol and low density lipid (Vajpeyiet al., 2005). Linseed oil is a triglyceride, like other fats. Linseed oil is
distinctive for its unusually large amount of α-linolenic acid, which has a distinctive reaction with oxygen in air.
Specifically, the fatty acids in a typical linseed oil are the tri -polyunsaturated α-linolenic acid (51.9-55.2%), the
saturated acids palmitic acid (about 7%) and stearic acid (3.4-4.6%), the monounsaturated oleic acid (18.5-22.6%),
the doubly unsaturated linoleic acid (14.2-17%). Among oilseed crops grown in India during Rabi. Linseed is next
in importance to rapeseed- mustard in acreage as well as in production. India stands at fourth position in global
production (Anonymous, 2013). Linseed is known to suffer from many diseases such as blight, rust, canker,
damping off, leaf spot, root rot and wilt (Kolte and Fitt, 1997). Alternaria blight, earlier designated as a minor
disease has assumed greatest importance, in different parts of the country, especially mid eastern India. Alternaria
blight caused by Alternaria lini Dey and A. linicola Grooves and Skolko, is a major biotic stress, limiting crop
yield in hot and humid environment (Singh and Singh 2004, 2005).
Out of two species of Alternaria known to occur on this crop, Aternaria lini is a more severe one. Survey
of literature revealed that no information is available on variability of A. lini or A. linicola causing blight of linseed
in India or outside of country. Since still no true resistance is available against this disease, it indicates the presence
of variability in pathogen in different agro climatic zone of the country. To develop the resistant cultivar, it
www.tjprc.org [email protected]
362 A.K.Verma & R.B.Singh
requires stable resistant donor for this kind of variability in blight causing pathogen is necessary. Hence, present
investigation was undertaken with pathogenic and molecular variability.
MATERIALS AND METHOD
The experiment was carried out in glass house, laboratories of Department of Plant Pathology and Plant Molecular
Biology & Genetic Engineering (PMB&GE) of Narendra Deva University of Agriculture and Technology, Kumarganj,
Faizabad (U. P.) during year 2012-13 and 2013-14. Samples were collected from different agro climatic zones of India
(Patna, Bilaspur, Sultanpur, Mauranipur, Mashodha, Kanpur, Kangra, Ranchi, Ranchi, Mirzapur, Gorakhpur, Berhampur,
Kumarganj and Jammu). Potato-Dextrose-Agar medium was used for maintaining of pure culture of Alternariaspecies.
Different media viz., Radish agar medium, Maize meal medium, Barley meal medium, Linseed leaf extract medium, Carrot
agar medium as well as Potato Dextrose Agar medium were used for cultural studies of the A. lini. Effect of different
culture media on colony colour, appearance, pattern of growth, shape, margin, zonatoin, sporulation and radial growth of
12 isolates of Alternarialini on potato dextrose agar medium, radish agar medium, maize meal medium, barley meal
medium, linseed leaves extract medium and carrot agar medium were observed from 7 days old culture. Morphological
variability of 12 isolates were studied. The different characters viz. size of conidiophores and conidial characters such as
length, width, colour, shape, and number of septa were studied on Potato Dextrose Agar (PDA) medium. The size of
conidiophores and conidia were examined under microscope using ocular micrometry (Meenaet al. 2005). Visual
observation for septation was done by using light microscope (40X) taking 20 conidia from each slide. Length range and
average length, width range and average width, range of horizontal septa of conidia and conidiophores were taken.
RESULTS AND DISCUSSIONS

Cultural variability
Variability of 12 isolates collected from different places studied by culturing them on different media (Potato
dextrose agar medium, Barley meal medium, Radish agar medium, Carrot agar medium, Maize meal medium, and Linseed
leaf extract medium) to find out their growth pattern, colonies colour, appearance, shape, margin, zonation and sporulation.
The results obtained from the studies are discussed as under:
On potato dextrose agar medium the colonies colours of different isolates were noted as whitish, dirty white, light
brown and dark brown. Variation on colonies colour of different isolates of Alternaria solani were studied by Kumar et al.
(2007) and Singh et al.(2014) in case of tomato and they also reported variable colonies colour, such as brownish black,
greenish black, reddish black, whitish black, and yellowish. Pramila et al. (2014) worked on variability of Alternaria
brassicae isolates of Uttarakhand and reported whitish to grayish colonies colours. The variation of colonies colours in
present study, supports the finding of above workers.
Besides PDA, colonies of isolates grown on radish agar medium showed dirty white (Al10), light brown (Al4, Al6,
Al7, Al8, Al9, Al11 and Al12) and brown (Al1, Al2, Al3 and Al5) colours. Isolates also showed variation on maize meal
medium as white (Al10), dirty white (Al1, Al2, and Al5) and light brown (Al3, Al4, Al6, Al8, Al9, Al11 and Al12) colonies.
On barley meal medium colonies colours of isolates were found dirty white (Al9), light brown (Al1, Al2, Al4, Al6, Al7, Al8,
Al10, and Al11) and brown (Al3, Al5, Al7 and Al12). Linseed leaf extract medium the colonies colours of 2 isolates (Al1 and
Al2) were recorded brown, 8 (Al3, Al4, Al5, Al6, Al7, Al9, Al10 and Al12) light brown, remaining 2 whitish (Al11) and dirty
white (Al8), respectively. On carrot agar medium variable colonies colours were also seen, in case of different isolates.
Impact Factor (JCC): 5.9857 NAAS Rating: 4.13

Cultural and Morphological Variability of Alternaria Lini 363
Isolates Causing Blight of Linseed
Seven isolates (Al4, Al6, Al7, Al8, Al9, Al10 and Al11) gave light brown, four (Al1, Al2, Al3 and Al5) brown and one (Al12)
dirty white colour (Table.1& Fig.1, 2,3,4,5 and 6). Sharma et al. (2013) studied the on cultural variability of different
isolates of A. brassicae, collected from different part of country, by using potato dextrose medium, cauliflower (host) agar
medium and carrot potato agar medium and reported variable colonies colour. Besides these, several worker working with
different species of Alternaria, causing blight disease in different crop have also studied the variability of colony colour on
different medium and reported variability in colour of colonies produced by different isolate time to time (Vishwanath,
1999, Kumar et al. 2007, Singh et al.2009, Khulbeet al. 2009, Meena et al. 2012, Pramila et al. 2014 and Singh et al.
2014). Variability in colonies colour, recorded in present studies supports the finding of earlier workers. The medium used
for the studies in case of Alternaria lini is of new kind because; it has not been used earlier by other workers in their
studies, in case of other Alternaria spp. causing disease in different crops.
Radial growth of colonies formed by different isolates of A. lini were recorded, after 7 days of inoculation and
found variable on different media. On potato dextrose agar medium radial growth of colonies, ranged between 25 mm
(Al12) to 52 mm (Al11), on radish agar medium from 25 mm (Al4) to 46 mm (Al10), on maize meal medium from 25 mm
(Al12) to 50 mm (Al5), on barley meal medium from 30 mm (Al12) to 50 mm (Al11), on linseed leaf extract medium from 38
mm (Al6) to 60 mm (Al11) and on carrot agar medium from 34 mm (Al12) to 48 mm (Al3). The isolates Al11 collected from
Berhampur (W. B.), showed maximum radial growth and isolate Al6 obtained from Kanpur (U.P.) showed minimum
growth on all the test media (Table 2 & Figure.1, 2,3,4,5 and 6). The shape of colonies formed by all the isolates on each
media was noted circular. Variations in colonies growth (slow medium and fast) were also noted in different isolates on all
the test media (Table.3& Figure.1, 2,3,4,5 and 6). All the isolates except Al11 showed smooth margin of the colonies during
their growth on potato dextrose agar medium, maize meal medium, linseed leaf extract medium, radish agar medium and
carrot agar medium. Isolate Al11 also showed smooth margin on these media except radish agar medium and maize meal
medium on which it showed rough margin. All the isolates showed wavy margin on barley meal medium. Colonies formed
by different isolates also showed variable appearance (cottony, feathery, fluffy and compressed & thin) during their
growth. On maize meal medium all the isolates showed compressed & thin appearance. It may be due less availability of
nutrients or organic matter in this crop (Table.4& Figure.1, 2,3,4,5 and 6). Most of the isolates under studies showed
zonation on different media, only few have not shown zonation on some media during their colonies growth. Sporulation in
different isolates on different media was also found variable. Some isolates showed early sporulation, some medium, some
showed late sporulation on different media. On PDA most of the isolates, showed early sporulation except Al4 and Al6
which showed medium sporulation. On maize meal, medium and barley meal medium all the isolates showed medium
sporulation. It is not as early as, in case of PDA. On radish agar medium all the isolates showed early sporulation except
Al6 and Al10. Isolates on rest of the media showed either early or medium or late sporulation.
Concurrent with present findings, studies were also carried out by several workers in case of Alternariabrassicae
isolates collected from different part of the country on variability of radial growth, colonies shape, colonies growth pattern,
margin, appearance, zonation and sporulation and they reported 28.20mm to 81.10 mm radial growth, circular to wavy
shape, feathery, fluffy to cottony appearance of colonies, presence or absence of zonation, and early, medium to late
sporulation on potato dextrose agar medium (Khulbeet al. 2009., Goyelet al.2011., Sharma et al.2013 and Pramila et
al.2014). Kumar et al. (2009) and Singh et al. (2014) have also obtained the variability in case of Alternariasolani isolates
and reported variation in radial growth (14.90 to 35.50), colour of colonies (brownish black, greenish black, redish black
and yellowish), shape (circular to irregular), growth pattern (slow, medium and fast), margin (smooth, rough and wavy),
appearance (fluffy, feathery and cottony), zonation (presence and absence) and sporulation (early, medium and late).
Table.1: Effect of Different Culture Media on Colony Colour of Different Isolates of Alternaria Lini
Linseed
Radish Barley Carrot
Maize Meal Leaf
Media PDA Agar Meal Agar
Medium Extract
Isolates Medium Medium Medium
Medium
Light
Al1 Brown Brown Dirty white Brown Brown
brown
Light
Al2 Brown Brown Dirty white Brown Brown
brown
Light Light
Al3 Brown Light brown Brown Brown
brown brown
Light Light Light
Al4 Dirty white Light brown Light brown
brown brown brown
Light Light
Al5 Brown Dirty white Brown Brown
brown brown
Light Light Light
brown brown brown
Light Light Light
Al7 Light brown Brown Light brown
brown brown brown
Light Light Dirty
Al8 Whitish Light brown Light brown
brown brown white
Light Dirty Light
brown white brown
Dirty Light Light
Al10 Dirty white Whitish Light brown
white brown brown
Light Light Light
Al11 Light brown Whitish Light brown
brown brown brown
Light Light Light
Al12 Light brown Brown Dirty white
brown brown brown
Table 2: Effect of Different Culture Media on the Mycelial Growth of

Different Isolates of Alternaria Lini (Mm.)
Linseed
Radish Maize Barley Carrot
Media Leaf
PDA Agar Meal Meal Agar
Isolates Extract
Medium Medium Medium Medium
Medium
Al1 45 44 45 40 53 45
Al2 45 44 45 42 52 44
Al3 45 45 50 47 55 48
Al4 35 25 28 30 40 34
Al5 45 45 50 48 54 47
Al6 34 26 28 30 38 34
Al7 45 46 45 40 42 44
Al8 44 45 44 42 40 42
Al9 45 44 46 42 42 44
Al10 46 46 45 40 40 40
Al11 52 40 45 50 60 40

Table 2: Contd.,
Al12 25 28 25 30 45 34
S.Em± 0.82 0.82 0.88 0.82 0.86 0.82
C.D. at
2.28 2.28 2.47 2.28 2.44 2.28
1%
Table .3 Variations in Colonies Growth of Different Isolates of

Alternaria Lini on Different Culture Media
Linseed
Radish Maize Barley Carrot
Media Leaf
PDA Agar Meal Meal Agar
Isolates Extract
Medium
Al1 Medium Medium Medium Medium Fast Medium
Al2 Medium Medium Medium Medium Fast Medium
Al3 Medium Medium Fast Medium Fast Medium
Al4 Slow Slow Slow Slow Medium Slow
Al5 Medium Medium Fast Medium Fast Medium
Al7 Medium Slow Medium Medium Medium Medium
Al8 Medium Medium Medium Medium Medium Medium
Al11 Fast Medium Medium Fast fast Medium
Table.4: Effect of Different Culture Media on the Appearance of Colonies of Alternaria Lini Isolates
Radish Barley Linseed Leaf Carrot
Media Maize Meal
PDA Agar Meal Extract Agar
Isolates Medium
Thick Compressed
AI1 Cottony Cottony Cottony Cottony
cottony and thin
Thick Compressed
Al2 Cottony Cottony Cottony Cottony
cottony and thin
Compressed
Al3 Cottony Cottony Cottony Cottony Feathery
and thin
Compressed
Al4 Feathery Cottony Cottony Cottony Cottony
and thin
Compressed
Al5 Cottony Cottony Feathery Cottony Feathery
and thin
Compressed
Al6 Cottony Cottony Cottony Cottony Cottony
and thin
Compressed
Al7 Cottony Cottony Fluffy Cottony Feathery
and thin
Compressed
Al8 Cottony Feathery Cottony Cottony Feathery
and thin
Compressed
Al9 Cottony Cottony Cottony Cottony Feathery
and thin
Table 4: Contd.,
Compressed
Al10 Feathery Cottony Cottony Cottony Feathery
and thin
Compressed
Al11 Cottony Cottony Fluffy Cottony Cottony
and thin
Compressed
Al12 Cottony Cottony Cottony Cottony Cottony
and thin
Al1 AI2 AI3 AI4 AI5 AI6
AI7 AI8 AI9 AI10 AI11 AI12
Figure 1: Colony Morphology of A. Lini Isolates on Carrot Agar Medium (Al1.Patna, Al2.Bilaspur,
Al3.Sultanpur, Al4.Mauranipur, Al5.Mashodha, Al6.Kanpur, Al7.Kangra, Al8.Ranchi,
Al9.Mirzapur, Al10.Gorkhpur, Al11.Berhampur, Al12.Kumarganj)
Figure.2: Colony Morphology of A. Lini Isolates on Potato Dextrose Agar Medium (Al1.Patna, Al2.Bilaspur,
Al9.Mirzapur, Al10.Gorkhpur, Al11.Berhampur, Al12. Kumarganj)

Figure 3: Colony morphology of A. lini isolates on Barley meal medium (Al1.Patna, Al2.Bilaspur, Al3.Sultanpur,
Al4.Mauranipur, Al5.Mashodha, Al6.Kanpur, Al7.Kangra, Al8.Ranchi,
Figure 4: Colony Morphology of A. Lini Isolates on Maize Meal Medium (Al1.Patna, Al2.Bilaspur,
Figure 5: Colony morphology of A. lini isolates on Radish agar medium (Al1.Patna, Al2.Bilaspur,
Figure 6: Colony Morphology of A. Lini Isolates on Linseed Leaf Extract Medium (Al1.Patna, Al2.Bilaspur,
Morphological variability
Besides cultural variability of isolates on different culture media, morphological variability of conidia and
conidiophores produced by them were also studied and discussed. Average length of conidia of Alternarialini, obtained
from different isolates ranged between 23.26 µm (Al7 of Kangra) to 45.72 µm (Al11of Berhampur) and width between 6.76
µm (Al4 of Mauranipur) to 17.01 µm (Al11 of Berhampur). Horizontal septa in conidia, obtained from different isolates
were also found variable, which ranged between, on basis of mean from 1.86 (Al8 of Ranchi) to 5.60 (Al11 of Berhampur).
Likewise, colonies colour and shape of conidia, also found variable in different isolates. Most of the conidia were found
light brown colour (Al1, Al2, Al3, Al4, Al6, Al9, Al11 and Al12) and few were dark brown in colour (Al5, Al7, Al8, and Al10).
Oval shape of conidia were observed in isolates Al1, Al2, Al4, Al5, Al6, Al7 and Al10 obclavate type in isolates Al3, Al8 and
Al9, while Al11 and Al12 were noted clavate type. In general, the conidia obtained from most of the isolates on PDA were
unbeaked, some were with rudimentary beaked and few were short beaked. Unbeaked conidia were obtained from isolates
Al1, Al2, Al4, Al5, Al6, Al7, Al8, and Al11, short beaked from Al3 and Al9, while rudimentary beaked from Al11 and
Al12,respectively(Table.5 and Fig.7). Concurrent with present finding Kumar et al. (2003) have also reported distinct
appearance among the isolates of Alternaria brassicae of rapeseed- mustard, in terms of conidial length, width and number
of septation at Haryana. They reported that average conidial length varied appreciably from 118.20µm to 194.52 µm and
identified 8 pathotypes. Mehta et al. (2003) worked on the morphological and pathological variation in rapeseed- mustard
isolates of A. brassicae collected from different agroclimatic zone of India. They also reported variations in spore length
and width, and indicated the existence of variability in the pathogen. Khan et al. (2007) isolated A. brassicae from the
samples collected from different places of Aligarh district and reported variations in conidial length (112 to 185.60 µm),
width (14.40 µm to 17.60 µm) and septation (5 to 16). Singh et al. (2009) also studied on morphological variation among
105 isolates A. brassicae collected from 18 district of Haryana. They found that, average conidial length and width ranged
from 117.00 to 192 µm and 14.0 to 24 µm, respectively. Length of beak, horizontal septa and vertical septa varied from
42.0 to 116.0 µm, 6 to 9 and 1 to 3, respectively. Goyalet al. (2011) and Sharma et al. (2013), also found variable
morphology (length, width and number of septa) in different isolates of A. brassicae collected from different parts of
country. Khulbe et al. (2011) and Pramila et al. (2014), studied the variability in different isolates of A. brassicae, collected
from different part of Uttarakhand. They reported that conidial size ranged from 55.23 to 152.17 µm × 12.00 to 88.40 µm
and 105 to 135 × 10 to 20 µm, respectively. The variation in conidial length, width, septa and beak, in case of A. lini in
present studies supports the views of earlier workers. The studies therefore, indicate the existence of variability among the
A. lini isolates collected from different part of country.

Like the conidial characters of A. lini morphological variability in conidiophores, were also recorded in respect of
length, width, colour and number of septa. Average length and width of conidiophores, ranged between 33.00 µm (Al7) to
81.50 µm (Al12) and 4.45 µm (Al7) to 9.27 µm (Al3), respectively. Colour of conidiophores was observed light brown to
dark brown. Average septation in conidiophores, ranged between 3.33 (Al7 of Kangra) to 7.53 (Al12 of Kumarganj)
(Table.6).
As per literature available, it was noted that most of the earlier pathologist working with different Alternariaspp.,
have studied the variations in conidial characters only. None have taken into consideration, about the variation in
characters of conidiophores. During present studies, variation in characters of conidiophores have also been taken into
consideration and found variable.
Table 5: Morphological Characters of Conidia
Length (µm ) Width (µm ) Horizontal septa

Different
Colour Shape Beak
isolates Ave Ave Rang
Range Range Average
rage rage e
22.50- 27.3 14.00- 15.0 Light
Al1 oval 2-3 2.60 Unbeaked
30.00 3 15.50 0 brown
22.50- 23.7 11.30- 14.0 Light
26.50 3 15.00 1 brown
22.50- 28.0 7.50- Dark obclav Short
Al3 9.02 2-3 2.40
30.00 0 11.33 brown ate beaked
30.00- 30.7 Light
Al4 3.80-7.50 6.76 oval 2-3 2.33 Unbeaked
33.80 6 brown
22.50- 26.5 7.50- Dark
Al5 8.70 oval 3-4 3.40 Unbeaked
30.00 0 11.30 brown
22.50- 28.0 7.50- Light
30.00 0 11.30 brown
22.50- 23.2 15.00- 15.7 Dark
26.30 6 18.80 6 brown
30.00- 31.0 Dark obclav
Al8 7.0-8.0 7.50 1-2 1.86 Unbeaked
33.80 1 brown ate
22.50- 24.0 15.00- 16.0 Light obclav Short
Al9 3-4 3.53
26.30 4 18.80 6 brown ate beaked
22.50- 27.0 7.50- Dark
30.00 0 11.30 brown
45.00- 45.7 15.00- 17.0 Light Rudimentar
Al11 clavate 5-7 5.60
48.00 2 18.80 1 brown y beaked
22.50- 25.5 7.50- 10.0 Dark Rudimento
Al12 clavate 2-3 2.52
30.00 0 11.30 0 brown ry beaked
S.Em± 0.62 0.30 0.17
C.D.at
1.74 0.83 0.48
1%
Table 6: Morphological Characters of Conidiophores
Length (µm ) Width (µm ) Horizontal septa

Different
Colour
isolates Ave
Range Range Average Range Average
rage
63.5
Al1 60.00-67.50 3.80-7.50 5.52 Dark brown 4-5 4.26
0
62.5
Al2 60.00-67.50 3.80-7.50 5.77 Dark brown 4-5 4.26
0
Table 6: Contd.,
79.5 7.50-
Al3 75.00-90.00 9.27 Dark brown 6-8 6.83
0 11.30
56.5
Al4 52.50-60.00 6.00-7.50 7.10 Light brown 5-7 6.09
0
78.0 7.50-
Al5 75.00-82.90 9.02 Dark brown 6-8 6.86
0 11.30
46.2
Al6 45.00-48.80 6.00-7.50 7.10 Light Brown 3-4 3.53
6
33.0
Al7 30.00-37.50 3.80-7.50 4.54 Light brown 3-4 3.33
0
36.0
Al8 33.80-37.50 6.00-7.50 7.00 Dark brown 3-4 3.40
0
45.2 7.50-
Al9 41.30-48.80 9.02 Light brown 4-6 3.86
6 11.30
44.5 7.50-
Al10 41.30-48.80 8.76 Light brown 3-5 3.40
0 11.30
77.8 7.50-
Al11 75.00-90.00 8.26 Dark brown 6-8 6.80
3 11.30
81.5 7.50-
Al12 75.00-90.00 8.51 Dark brown 7-9 7.53
0 11.30
S.Em± 0.30 0.44 0.45
C.D.at
0.87 1.23 0.61
1%
Patna isolate (Al1) Bilaspur isolate (Al2) Sultanpur isolate (Al3 )
Mauranipur isolate (Al4 ) Mashodha isolate (Al5) Kanpur isolate (Al6)
Kangra isolate (Al7) Ranchi isolate (Al8) Mirzapur isolate (Al9)

Gorakhpur Isolate (Al10) Berhampur Isolate (Al11 ) Kumarganj isolate (Al12)
Figure 7: Morphological Characters of Isolates (Al1,Al2,Al3,Al4,Al5, Al6, Al7,Al8,Al9,Al10,Al11 And Al12)
CONCLUSIONS
The diseased samples were collected from thirteen places of India. Out of thirteen samples, pathogen causing
disease could be isolated successfully from 12 samples and one obtained from Jammu (J&K) could not be isolated. These
isolates were purified by single spore technique method and identified as Alternaria lini. All the isolates were cultured
separately on different media and showed variable reaction in respect of colonies colour, appearance, growth pattern, shape
of colonies, margin, zonation, sporulation and radial growth. On all the test media the colonies colours of different isolates
were observed as whitish, dirty white, light brown and dark brown. Radial growths of colonies formed by different isolates
of A.lini were recorded after 7 days of inoculation found variable. Isolates Al11 collected from Berhampur (W.B.) showed
maximum radial growth and isolates Al6 obtained from Kanpur (U.P.) showed minimum radial growth on all the test
media. Shape of colonies formed by all the isolates on each media was circular. Colonies growths were varied from slow to
fast on different culture media in case of all the isolates. Sporulation in different isolates on different culture media was
also found variable. Some isolates showed early sporulation, some medium and some late on different media. Appearances
of colonies formed on different media by different isolates were cottony, feathery, fluffy or compressed & thin. Average
length of conidia of A. lini obtained from different isolates, ranged between 23.26µm (Al7 of Kangra) to 45.72 µm (Al11 of
Berhampur) and width between 6.67 µm (Al4 of Mauranipur) to 17.01 µm (Al11 of Berhampur). Average number of
horizontal septa, in conidia of different isolates ranged between 1.86 (Al8 of Ranchi) to 5.60 (Al11 of Berhampur). Most of
conidia obtained from different isolates were found light brown and few were dark brown in colour. Shape of conidia were
also found variable and noted as oval, obclavate and clavate type. The conidia obtained from most of the isolates on PDA
were unbeaked (Al1, Al2, Al4, Al5, Al6, Al7, Al8, Al10), some were with rudimentary beaked (Al11 and Al12) and few were
short beaked (Al3 and Al9). Morphological characters of conidiophores were also found variable in respect of length (33.00
to 81.50µm), width (4.54 to 9.27µm), colour (light brown to dark brown) and number of septa (3.33 to 7.53).
REFERENCES
1. Anonymous (2013).Annual Progress Report 2013-14. All India Coordinated Research Project on oilseeds (linseed).
Directorate of Oilseed Research, Rajendranagar, Hyderabad, pp. 177.
2. Goyal, P., Chahar, M., Mathur, A.P., Kumar, A. and Chattopadhyay, C. (2011). Morphological andcultural variation in
different oilseed Brassica isolates of Alternariabrassicaefrom different geographical regions of India. Indian Journal of
Agricultural Sciences. 81 (11): 1052–1058.
3. Khulbe, A., Awasthi, R.P. and Tewari, A.K. (2011).Morphological and cultural diversity in isolates of Alternariabrassicae
infecting rapeseed and mustard.Pantnagar Journal of Research.9 (2): 206-209.
4. Kolte, S.J. and Fitt, B.D.L. (1997).“Disease of Linseed and Fibre Flax.”Shipra Publications, Delhi, pp. 247.
5. Kumar,V., Haldar,S., Pandey, K.K.,Singh, R.P., Singh, A.K. and Singh, P.C. (2007). Cultural, morphological,pathogenic and
molecular variability amongst tomato isolates of Alternariasolani in India. World Journal of Microbiology and
Biotechnology.24: 1003-1009.
6. Akash Verma, Sobita Simon, Kamaluddeen & Yojna Lal, Efficacy of Indigenous Products and Carbendazim on Alternaria
Blight [(Alternaria Lini) Dey] of Linseed (Linum Usitatissimum L.) Under Allahabad Conditions, International Journal of
Agricultural Science and Research (IJASR), Volume 3, Issue 5, November - December 2013, pp. 33-38
7. Meena, P.D., Chattopadhyay, C., Kumar, V.R., Meena, R.L. and Rana, U.S. (2005). Spore behavior in atmosphere and trends
in variability of AlternariabrassicaePopulation in India.Journal of Mycology and Plant Pathology.35 (3): 511.
8. Pramila.,Giri,K., Tasleem, M., Taj,G., Mal,R. and Kumar,A. (2014). Morphological, cultural, pathogenic and molecular
variability amongst Indian mustard isolates of Alternariabrassicae in Uttarakhand. African Journal of Biotechnology.13(3):
441-448.
9. Sharma, M., Deep,S., Bhati, D. S.,Chowdappa, P., Selvamani, R. and Sharma, P. (2013). Morphological, cultural, pathogenic
and molecular studies of Alternaria brassicae infecting cauliflower and mustard in India. African Journal of Microbiology
Research.7 (26):3351-3363.
10. Singh, R. B. and Singh, R. N. (2004).Management of alternaria blight of linseed. Annals of Plant Protection Sciences.12(2):
305-309.
11. Singh,A., Singh,V. and Yadav,S.M. (2014). Cultural, morphological and pathogenic variability of Alternariasolani causing
early blight in tomato.Science Alert.13: 167-172.
12. Vajpeyi, M., Dubey, S.D. and Srivastava, R.L. (2005).Linseed : Industrial and Medicinal uses. AICRP on linseed, P.C. Unit
(linseed) Kanpur.pp.16

Cultural and Morphological Variability of Isolates Causing Blight of Linseed

Uploaded by

Copyright:

Available Formats

Cultural and Morphological Variability of Isolates Causing Blight of Linseed

Uploaded by

Document Information

Original Title

Copyright

Available Formats

Share this document

Share or Embed Document

Sharing Options

Did you find this document useful?

Is this content inappropriate?

Copyright:

Available Formats

Cultural and Morphological Variability of Isolates Causing Blight of Linseed

Uploaded by

Copyright:

Available Formats

International Journal of Agricultural

Science and Research (IJASR)

CULTURAL AND MORPHOLOGICAL VARIABILITY OF ALTERNARIA LINI

ISOLATES CAUSING BLIGHT OF LINSEED.

A.K.VERMA & R.B.SINGH

MATERIALS AND METHOD

RESULTS AND DISCUSSIONS

Impact Factor (JCC): 5.9857 NAAS Rating: 4.13

Table 2: Effect of Different Culture Media on the Mycelial Growth of

Impact Factor (JCC): 5.9857 NAAS Rating: 4.13

Table .3 Variations in Colonies Growth of Different Isolates of

Al1 AI2 AI3 AI4 AI5 AI6

AI7 AI8 AI9 AI10 AI11 AI12

AI1 AI2 AI3 AI4 AI5 AI6

AI7 AI8 AI9 AI10 AI11 AI12

AI1 AI2 AI3 AI4 AI5 AI6

Impact Factor (JCC): 5.9857 NAAS Rating: 4.13

AI7 AI8 AI9 AI10 AI11 AI12

AI1 AI2 AI3 AI4 AI5 AI6

AI7 AI8 AI9 AI10 AI11 AI12

AI1 AI2 AI3 AI4 AI5 AI6

AI7 AI8 AI9 AI10 AI11 AI12

AI1 AI2 AI3 AI4 AI5 AI6

AI7 AI8 AI9 AI10 AI11 AI12

Impact Factor (JCC): 5.9857 NAAS Rating: 4.13

Table 5: Morphological Characters of Conidia

Length (µm ) Width (µm ) Horizontal septa

Table 6: Morphological Characters of Conidiophores

Length (µm ) Width (µm ) Horizontal septa

Patna isolate (Al1) Bilaspur isolate (Al2) Sultanpur isolate (Al3 )

Mauranipur isolate (Al4 ) Mashodha isolate (Al5) Kanpur isolate (Al6)

Kangra isolate (Al7) Ranchi isolate (Al8) Mirzapur isolate (Al9)

Impact Factor (JCC): 5.9857 NAAS Rating: 4.13

Gorakhpur Isolate (Al10) Berhampur Isolate (Al11 ) Kumarganj isolate (Al12)

Figure 7: Morphological Characters of Isolates (Al1,Al2,Al3,Al4,Al5, Al6, Al7,Al8,Al9,Al10,Al11 And Al12)

Impact Factor (JCC): 5.9857 NAAS Rating: 4.13

You might also like