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    Date: Experiment No 9 Determination of K A by Sulphite Oxidation Method

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    This document describes an experiment to determine the oxygen mass transfer coefficient (KLa) using a sodium sulphite oxidation method. Sodium sulphite reacts with oxygen in the presence of copper sulfate as a catalyst. Samples are taken from the reactor over time and titrated to determine the remaining sodium sulphite. The rate of oxygen transfer is calculated from the slope of a graph of titration volumes over time. This rate is used to calculate KLa, which characterizes a bioreactor's capacity for aeration and oxygen mass transfer.

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    Date: Experiment No 9 Determination of K A by Sulphite Oxidation Method

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    anon_64239065
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    This document describes an experiment to determine the oxygen mass transfer coefficient (KLa) using a sodium sulphite oxidation method. Sodium sulphite reacts with oxygen in the presence of copper sulfate as a catalyst. Samples are taken from the reactor over time and titrated to determine the remaining sodium sulphite. The rate of oxygen transfer is calculated from the slope of a graph of titration volumes over time. This rate is used to calculate KLa, which characterizes a bioreactor's capacity for aeration and oxygen mass transfer.

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    This document describes an experiment to determine the oxygen mass transfer coefficient (KLa) using a sodium sulphite oxidation method. Sodium sulphite reacts with oxygen in the presence of copper sulfate as a catalyst. Samples are taken from the reactor over time and titrated to determine the remaining sodium sulphite. The rate of oxygen transfer is calculated from the slope of a graph of titration volumes over time. This rate is used to calculate KLa, which characterizes a bioreactor's capacity for aeration and oxygen mass transfer.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    199 views4 pages

    Date: Experiment No 9 Determination of K A by Sulphite Oxidation Method

    Uploaded by

    anon_64239065
    This document describes an experiment to determine the oxygen mass transfer coefficient (KLa) using a sodium sulphite oxidation method. Sodium sulphite reacts with oxygen in the presence of copper sulfate as a catalyst. Samples are taken from the reactor over time and titrated to determine the remaining sodium sulphite. The rate of oxygen transfer is calculated from the slope of a graph of titration volumes over time. This rate is used to calculate KLa, which characterizes a bioreactor's capacity for aeration and oxygen mass transfer.

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    of 4

    Date:

    Experiment No 9

    DETERMINATION OF KLa BY SULPHITE OXIDATION METHOD

    AIM:
    To determine the oxygen mass transfer coefficient, KLa using sodium sulphite oxidation
    method.

    THEORY:
    The volumetric liquid mass transfer coefficient (KLa) is a useful parameter to characterize
    bioreactors capacity for aeration. This helps the reactor design, optimization of technologies and
    scaling up or scaling down processes. The sodium sulphite combines with oxygen to give sodium
    sulphate, with CuSO4 as catalyst in the reactor. The concentration of sodium sulphite at various
    time points is inversely proportional to the oxygen transfer rate.
    Cu2+
    Na2SO3 + ½ O2 Na2SO4
    The above reaction is
    A. Independent of sodium sulphite concentration within the range of 0.04N to 1.0N
    B. The rate of the reaction is much faster than O2 transfer rate.
    Thus, the rate of oxidation is controlled by rate of mass transfer alone.
    The reaction consumes oxygen at a rate that is sufficiently fast so that transport of O2 from gas to
    liquid through liquid film is the rate-limiting step. The rate of the reaction is zeroth order in
    Na2SO3. If the reaction is not fast enough the reaction occurs in the liquid film around the gas
    bubbles. This would decrease apparent film thickness and give incorrectly high values of K La.
    Concentrations of unreacted sulphite are determined by reacting the sulphite with excess iodine
    and then back titration of the iodine with thiosulphate. It is important to note that the dissolved
    oxygen is zero through the reaction.
    By titrating sodium sulphite present in the reactor (by taking sample at fixed intervals of time)
    against sodium thiosulphate, the quantity of sodium sulphite that would have reacted according
    to equation can be measured as the difference between successive rate instants of time. Then
    based on stoichiometry the corresponding number of moles of O2 that would have been
    consumed can be determined.
    The Oxygen transfer rate
    OTR = KLa (C*-CL)
    Where C* = 8.43X10-3g/l at 25oC
    CL = Dissolved Oxygen content.
    In this case, CL = 0, since the DO is maintained such that it is not saturated.
    OTR = KLa (C*) -------- (1)
    KLa = OTR/ C*
    Thus KLa can be determined.

    MATERIALS REQUIRED:
     0.5M Na2SO3
     1% starch solution
     0.5M Na2S2O7
     0.002M CuSO4.5H2O
     Standard Na2SO3 (0.1M to 0.5M)
     0.1M Iodine solution (20 g/l KI + 12.7 g/l I2)
     Measuring cylinder
     Distilled water
     Test tubes, eppendorfs, Burette, Conical flask, Magnetic stirrer etc.

    PROCEDURE:
     100 ml of 0.5M Sodium sulphite & 0.002M Copper sulphate solutions were taken in a
    250ml conical flask.
     The conical flask with Sodium sulphite solution was kept in magnetic stirrer for mixing
    and ‘0’ sample was taken for titration.
     15 mL of Iodine solution and few drops of starch indicator was added to the 1 ml of
    sample was taken from the flask.
     Sample solution was turned into dark blue colour and it was titrated against 0.5 N Sodium
    thiosulphate. (It becomes straw yellow color)
     The volume consumed are been noted.
     The same analysis has been repeated for the time of 5, 10, 15, 20 minutes samples.
     Graph was plotted between titer volumes versus time.
     OTR was calculated by using the slope of the graph.
     KLa value was calculated by using OTR & C* values.

    TABLE 1:TITRATION

    Sample time (min) Titer volume of


    Na2SO3.5H2O (ml)

    MODEL GRAPH:
    CALCULATION:

    RESULT:

    The value of KLa was estimated as _________________.

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